SitesBLAST

Q96XT4 2-oxoacid:ferredoxin oxidoreductase 2, subunit beta; OFOR2; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
37% identity, 87% coverage: 18:258/276 of query aligns to 11:245/304 of Q96XT4

query
sites
Q96XT4

W

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C

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L

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C

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E

C12 (= C19) binding [4Fe-4S] cluster
C15 (= C22) binding [4Fe-4S] cluster
IGCS 44:47 (= IGCS 51:54) binding thiamine diphosphate
C46 (= C53) binding [4Fe-4S] cluster
D90 (= D95) binding Mg(2+)
GD 91:92 (= GD 96:97) binding thiamine diphosphate
N118 (= N123) binding Mg(2+)
V120 (≠ I125) binding Mg(2+)
GL 122:123 (= GL 127:128) binding thiamine diphosphate
C197 (= C202) binding [4Fe-4S] cluster

5b47B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - pyruvate complex (see paper)
42% identity, 67% coverage: 18:202/276 of query aligns to 4:179/275 of 5b47B

query
sites
5b47B

W

W

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C

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C

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N

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Y

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C

binding magnesium ion: D83 (= D95), N111 (= N123)
binding iron/sulfur cluster: C5 (= C19), C8 (= C22), C39 (= C53), C179 (= C202)
binding thiamine diphosphate: I37 (= I51), G38 (= G52), C39 (= C53), S40 (= S54), H58 (= H70), G84 (= G96), D85 (= D97), N111 (= N123), V113 (≠ I125), Y114 (= Y126), L116 (= L128)

P72579 2-oxoacid:ferredoxin oxidoreductase subunit beta; OFOR; EC 1.2.7.11 from Sulfolobus sp. (see paper)
36% identity, 91% coverage: 18:268/276 of query aligns to 11:269/305 of P72579

query
sites
P72579

W

W

C

C

W

P

G

G

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C

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N

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K

K49 (≠ R56) mutation to I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to R: Increase the oxidoreductase activity with pyruvate.; mutation to V: Slight decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.
L123 (= L128) mutation L->A,I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to N: Strong decrease of the oxidoreductase activity with pyruvate and 2-oxobutyrate. However, this mutant shows almost the same activity with 2-oxoglutarate as the wild-type.

Q96Y68 2-oxoacid:ferredoxin oxidoreductase 1, subunit beta; OFOR1; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 2 papers)
36% identity, 91% coverage: 18:268/276 of query aligns to 11:269/305 of Q96Y68

query
sites
Q96Y68

W

W

C
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C

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C

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K

C12 (= C19) binding [4Fe-4S] cluster
C15 (= C22) binding [4Fe-4S] cluster
C46 (= C53) binding [4Fe-4S] cluster
K49 (≠ R56) mutation to I: Loss of oxidoreductase activity toward 2-oxoglutarate but retains its activity toward pyruvate.
D90 (= D95) binding Mg(2+)
GD 91:92 (= GD 96:97) binding thiamine diphosphate
N118 (= N123) binding Mg(2+)
V120 (≠ I125) binding Mg(2+)
GL 122:123 (= GL 127:128) binding thiamine diphosphate
K125 (≠ N130) Plays an important role in the binding of CoA; mutation to A: Shows a strong decrease of affinity for CoA and a poor inactivation by 4-fluoro-7-nitrobenzofurazan (NBD-F).
K173 (≠ L178) mutation to A: Same oxidoreductase activity as the wild-type.
C197 (= C202) binding [4Fe-4S] cluster

5b48B 2-oxoacid:ferredoxin oxidoreductase 1 from sulfolobus tokodai (see paper)
37% identity, 91% coverage: 18:268/276 of query aligns to 7:251/286 of 5b48B

query
sites
5b48B

W

W

C
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C

W

P

G

G

C
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C

G

G

D

N

G

F

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G

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A

A

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I

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G

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A

A

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A

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N

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G

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G

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A

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I

G

G

G

A

N

G

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A

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A

A

G

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R

N

N

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D

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L

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N

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N
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N

F

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I
x
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Y

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G

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L

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K

R

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G

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E

W

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-

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N

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L

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N

N

D

L

I

G

N

R

T

K

K

N

E

-

W

K

R

M

I

A

Y

K

K

A

L

K

D

Q

T

N

L

M

P

D

D

W

W

I

D

E

P

E

V

N

V

L

K

I

K

P

P

K

E

A

E

K

V

W

N

D

E

K

K

L

I

S

K

K

R

E

A

E

I

Q

D

Y

K

K

S

L

L

K

-

K

E

E

W

G

G

K

D

F

I

P

P

R

I

G

G

I

I

L

F

H

Y

H

Q

V

N

E

E

E

L

P

V

E

P

Y

S

C

Y

E

E

Y

R

Y

I

K

K

binding magnesium ion: D84 (= D95), N112 (= N123), V114 (≠ I125)
binding iron/sulfur cluster: C8 (= C19), C11 (= C22), C42 (= C53), C183 (= C202), T185 (≠ V204)
binding 2-[(2E)-3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-(1-oxidanylpropylidene)-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: I40 (= I51), G41 (= G52), C42 (= C53), S43 (= S54), H59 (= H70), G85 (= G96), D86 (= D97), N112 (= N123), V114 (≠ I125), Y115 (= Y126), G116 (= G127), L117 (= L128)

5exdF Crystal structure of oxalate oxidoreductase from moorella thermoacetica bound with carboxy-di-oxido-methyl-tpp (coom-tpp) intermediate (see paper)
25% identity, 67% coverage: 19:202/276 of query aligns to 24:225/310 of 5exdF

query
sites
5exdF

C
|
C

W

A

G

G

C
|
C

G

G

D
x
P

G

A

I

L

I

T

M

Y

K

R

A

L

M

V

I

A

R

K

A

A

F

A

A

G

K

P

-

N

-

T

-

I

-

F

L

I

G

G

W

P

D
x
T

K
x
G

N
x
C

D

M

I

Y

C

V

I

A

V

N

S

T

G

S

I

Y

G

G

C

C

S

G

G

P

R

W

F

R

S

V

S

P

Y

W

I

I

D

H

A

A

N

Q

T
x
I

V

T

H

N

-

G

-

A

T

V

T

A

H

S

G

G

R

I

T

E

V

A

A

A

Y

Y

A

K

T

A

G

M

I

I

K

R

L

K

A

K

N

K

P

T

D

D

-

A

-

E

-

F

K

P

H

N

V

I

I

I

V

V

V

M

A

A

G

G

D
|
D

G
|
G

D

G

G

A

L

V

A

D

I

I

G

G

G

L

N

Q

H

A

T

L

I

S

H

A

G

M

A

L

R

Y

R

R

N

G

I

H

D

D

L

V

N

L

F

F

V

I

L

C

I
x
Y

N

D

N
|
N

F

E

I
x
S

Y
|
Y

G
x
A

L
x
N

T
|
T

N

G

S

I

Q

Q

V

T

S

S

P

P

T

T

P

P

K

Y

G

G

M

A

W

N

T

T

V

T

T

F

T

T

Q

P

Y

P

G

G

N

E

I

V

D

V

P

P

T

E

-

G

-

K

-

L

F

F

D

P

A

K

C

D

D

N

L

P

A

K

I

V

G

I

A

A

G

H

A

G

-

H

-

P

-

E

-

L

T

K

F

Y

V

V

G

A

R

T

E

A

S

S

V

I

S

G

E

W

P

P

L

V

K

D

L

L

E

M

R

N

M

K

F

V

I

R

K

K

G

G

F

L

E

N

H

Q

K

E

G

G

F

P

S

A

F

Y

F

I

D

H

V

I

H

H

S

A

N

P

C
|
C

active site: N143 (≠ L128)
binding magnesium ion: D110 (= D95), N138 (= N123), S140 (≠ I125)
binding [2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-[1,1,2-tris(oxidanyl)-2-oxidanylidene-ethyl]-1,3-thiazol-3-ium-5-yl]ethoxy-oxidanyl-phosphoryl] hydrogen phosphate: T50 (≠ D41), G51 (≠ K42), C52 (≠ N43), I74 (≠ T65), D110 (= D95), G111 (= G96), Y136 (≠ I121), N138 (= N123), S140 (≠ I125), Y141 (= Y126), A142 (≠ G127), N143 (≠ L128), T144 (= T129)
binding iron/sulfur cluster: C24 (= C19), C27 (= C22), P29 (≠ D24), C52 (≠ N43), A142 (≠ G127), C225 (= C202)

Sites not aligning to the query:

binding iron/sulfur cluster: 227

5c4iC Structure of an oxalate oxidoreductase (see paper)
25% identity, 67% coverage: 19:202/276 of query aligns to 24:225/312 of 5c4iC

query
sites
5c4iC

C
|
C

W

A

G

G

C
|
C

G

G

D
x
P

G

A

I

L

I

T

M

Y

K

R

A

L

M

V

I

A

R

K

A

A

F

A

A

G

K

P

-

N

-

T

-

I

-

F

L

I

G

G

W

P

D
x
T

K
x
G

N
x
C

D

M

I

Y

C

V

I

A

V

N

S

T

G

S

I

Y

G

G

C

C

S

G

G

P

R

W

F

R

S

V

S

P

Y

W

I

I

D

H

A

A

N

Q

T
x
I

V

T

H

N

-

G

-

A

T

V

T

A

H

S

G

G

R

I

T

E

V

A

A

A

Y

Y

A

K

T

A

G

M

I

I

K

R

L

K

A

K

N

K

P

T

D

D

-

A

-

E

-

F

K

P

H

N

V

I

I

I

V

V

V

M

A

A

G
|
G

D
|
D

G
|
G

D

G

G

A

L

V

A

D

I

I

G

G

G

L

N

Q

H

A

T

L

I

S

H

A

G

M

A

L

R

Y

R

R

N

G

I

H

D

D

L

V

N

L

F

F

V

I

L

C

I
x
Y

N

D

N
|
N

F

E

I
x
S

Y
|
Y

G
x
A

L
x
N

T
|
T

N

G

S

I

Q

Q

V

T

S

S

P

P

T

T

P

P

K

Y

G

G

M

A

W

N

T

T

V

T

T

F

T

T

Q

P

Y

P

G

G

N

E

I

V

D

V

P

P

-

E

-

G

-

K

T

L

F

F

D

P

A

K

C

D

D

N

L

P

A

K

I

V

G

I

A

A

G

H

A

G

-

H

-

P

-

E

-

L

T

K

F

Y

V

V

G

A

R

T

E

A

S

S

V

I

S

G

E

W

P

P

L

V

K

D

L

L

E

M

R

N

M

K

F

V

I

R

K

K

G

G

F

L

E

N

H

Q

K

E

G

G

F

P

S

A

F

Y

F

I

D

H

V

I

H

H

S

A

N

P

C
|
C

active site: N143 (≠ L128)
binding magnesium ion: D110 (= D95), N138 (= N123), S140 (≠ I125)
binding iron/sulfur cluster: C24 (= C19), C27 (= C22), P29 (≠ D24), C52 (≠ N43), A142 (≠ G127), C225 (= C202)
binding thiamine diphosphate: T50 (≠ D41), G51 (≠ K42), C52 (≠ N43), I74 (≠ T65), G109 (= G94), D110 (= D95), G111 (= G96), Y136 (≠ I121), N138 (= N123), S140 (≠ I125), Y141 (= Y126), A142 (≠ G127), N143 (≠ L128), T144 (= T129)

Sites not aligning to the query:

binding iron/sulfur cluster: 226, 227

5exeC Crystal structure of oxalate oxidoreductase from moorella thermoacetica bound with carboxy-tpp adduct (see paper)
25% identity, 67% coverage: 19:202/276 of query aligns to 24:225/314 of 5exeC

query
sites
5exeC

C
|
C

W

A

G

G

C
|
C

G

G

D
x
P

G

A

I

L

I

T

M

Y

K

R

A

L

M

V

I

A

R

K

A

A

F

A

A

G

K

P

-

N

-

T

-

I

-

F

L

I

G

G

W

P

D
x
T

K
x
G

N
x
C

D

M

I

Y

C

V

I

A

V

N

S

T

G

S

I

Y

G

G

C

C

S

G

G

P

R

W

F

R

S

V

S

P

Y

W

I

I

D

H

A

A

N

Q

T
x
I

V

T

H

N

-

G

-

A

T

V

T

A

H

S

G

G

R

I

T

E

V

A

A

A

Y

Y

A

K

T

A

G

M

I

I

K

R

L

K

A

K

N

K

P

T

D

D

-

A

-

E

-

F

K

P

H

N

V

I

I

I

V

V

V

M

A

A

G
|
G

D
|
D

G
|
G

D

G

G

A

L

V

A

D

I

I

G

G

G

L

N

Q

H

A

T

L

I

S

H

A

G

M

A

L

R

Y

R

R

N

G

I

H

D
|
D

L

V

N

L

F

F

V

I

L

C

I
x
Y

N

D

N
|
N

F

E

I
x
S

Y
|
Y

G
x
A

L
x
N

T
|
T

N

G

S

I

Q

Q

V

T

S

S

P

P

T

T

P

P

K

Y

G

G

M

A

W

N

T

T

V

T

T

F

T

T

Q

P

Y

P

G

G

N

E

I

V

D

V

P

P

-

E

-

G

-

K

T

L

F

F

D

P

A

K

C

D

D

N

L

P

A

K

I

V

G

I

A

A

G

H

A

G

-

H

-

P

-

E

-

L

T

K

F

Y

V

V

G

A

R

T

E

A

S

S

V

I

S

G

E

W

P

P

L

V

K

D

L

L

E

M

R

N

M

K

F

V

I

R

K

K

G

G

F
x
L

E

N

H
x
Q

K

E

G

G

F

P

S

A

F

Y

F

I

D

H

V

I

H

H

S

A

N

P

C
|
C

active site: N143 (≠ L128)
binding [2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-carboxy-4-methyl-1,3-thiazol-3-ium-5-yl]ethoxy-oxidanyl-phosphoryl] hydrogen phosphate: T50 (≠ D41), G51 (≠ K42), C52 (≠ N43), I74 (≠ T65), G109 (= G94), D110 (= D95), G111 (= G96), Y136 (≠ I121), N138 (= N123), S140 (≠ I125), Y141 (= Y126), A142 (≠ G127), N143 (≠ L128), T144 (= T129)
binding magnesium ion: D110 (= D95), D130 (= D115), N138 (= N123), S140 (≠ I125), L211 (≠ F188), Q213 (≠ H190)
binding iron/sulfur cluster: C24 (= C19), C27 (= C22), P29 (≠ D24), C52 (≠ N43), C225 (= C202)

Sites not aligning to the query:

binding iron/sulfur cluster: 226, 227

Query Sequence

>WP_007474176.1 NCBI__GCF_000170735.1:WP_007474176.1
MAFNYDYYLRTDKMPTLWCWGCGDGIIMKAMIRAFAKLGWDKNDICIVSGIGCSGRFSSY
IDANTVHTTHGRTVAYATGIKLANPDKHVIVVAGDGDGLAIGGNHTIHGARRNIDLNFVL
INNFIYGLTNSQVSPTTPKGMWTVTTQYGNIDPTFDACDLAIGAGATFVGRESVSEPLKL
ERMFIKGFEHKGFSFFDVHSNCHVNLGRKNKMAKAKQNMDWIEENLIPKAKWDKLSKEEQ
YKLKKEGKFPRGILHHVEEPEYCEEYYKMVEKVRGN

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory