SitesBLAST

5exdH Crystal structure of oxalate oxidoreductase from moorella thermoacetica bound with carboxy-di-oxido-methyl-tpp (coom-tpp) intermediate (see paper)
36% identity, 58% coverage: 42:117/132 of query aligns to 223:286/291 of 5exdH

query
sites
5exdH

A

G

D

N

W

W

R

R

V

I

E

Q

K

R

P
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P

V

I

F

I

N

D

R

R

E

E

L

A

C
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C

I
x
T

D
x
E

C
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C

D
x
Y

F

T

C
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C

W

W

V

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S

Y

C
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C

P

P

D

D

S

S

C

C

F

I

L

T

V

R

E

T

E

E

V

-

N

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K

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-

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E

A

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x
P

I

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V

F

G

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I

M

N

K

Y

Y

N

-

L

-

C
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C

K
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K

G
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G

C
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C

A
x
G

V

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C

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E

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C

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x
S

P

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I

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K

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S

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L
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L

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E

binding iron/sulfur cluster: P230 (= P49), C237 (= C56), T238 (≠ I57), E239 (≠ D58), C240 (= C59), Y241 (≠ D60), C243 (= C62), C247 (= C66), P259 (≠ K86), C266 (= C95), K267 (= K96), G268 (= G97), C269 (= C98), G270 (≠ A99), C272 (= C101), C276 (= C105), S278 (≠ T107), L281 (= L112)

8e9iI Mycobacterial respiratory complex i, semi-inserted quinone (see paper)
35% identity, 58% coverage: 54:130/132 of query aligns to 50:132/166 of 8e9iI

query
sites
8e9iI

E

E

L

K

C
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C

I
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I

D
x
G

C
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C

D
x
E

F
x
L

C
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C

W

A

V

W

S

A

C
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C

P

P

D

A

S

D

C

A

F
x
I

L

Y

V

V

E

E

E

G

V

A

V

D

N

N

K

T

R

A

-

D

-

E

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R

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Y

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S

-

P

G

G

K

E

K

R

Q

Y

A

G

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V
x
Y

G

Q

I

I

N

N

Y

Y

N

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C

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x
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G

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x
G

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C

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E

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C

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P

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P

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I

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K

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L

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M

M

F

T

P

T

E

E

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Y

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E

-

M

M

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D

N

D

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N

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R

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A

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K

binding iron/sulfur cluster: C52 (= C56), I53 (= I57), G54 (≠ D58), C55 (= C59), E56 (≠ D60), L57 (≠ F61), C58 (= C62), C62 (= C66), I67 (≠ F71), Y90 (≠ V88), C97 (= C95), I98 (≠ K96), G99 (= G97), C100 (= C98), G101 (≠ A99), C103 (= C101), C107 (= C105), P108 (= P106)

Sites not aligning to the query:

binding iron/sulfur cluster: 40

7p63I Complex i from e. Coli, ddm/lmng-purified, under turnover at ph 6, closed state (see paper)
34% identity, 42% coverage: 52:107/132 of query aligns to 56:111/180 of 7p63I

query
sites
7p63I

N

G

R

E

E

E

L

R

C
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C

I
x
V

D
x
A

C
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C

D
x
N

F

L

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C

W

A

V

V

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A

C
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C

P

P

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S

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C

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x
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N

T

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K

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D

G

G

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R

K

W

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Y

A

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x
F

G

R

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I

N

N

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x
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C

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x
G

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x
L

C
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C

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E

E

E

V

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C
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C

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P

T
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T

binding iron/sulfur cluster: C60 (= C56), V61 (≠ I57), A62 (≠ D58), C63 (= C59), N64 (≠ D60), C66 (= C62), C70 (= C66), C74 (= C70), I75 (≠ F71), F92 (≠ V88), C99 (= C95), I100 (≠ K96), F101 (≠ G97), C102 (= C98), G103 (≠ A99), L104 (≠ V100), C105 (= C101), C109 (= C105), P110 (= P106), T111 (= T107)

Sites not aligning to the query:

binding iron/sulfur cluster: 48, 114

6ciqA Pyruvate:ferredoxin oxidoreductase from moorella thermoacetica with coenzyme a bound (see paper)
27% identity, 60% coverage: 46:124/132 of query aligns to 675:772/1169 of 6ciqA

query
sites
6ciqA

V

V

E

N

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P

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F
x
W

N

Q

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P

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E

L

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C

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I

D

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C

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x
N

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C

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C

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A

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N

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L

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C

K
x
T

G
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G

C
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C

A
x
G

V

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C
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C

V

A

E

D

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V

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C

P

P

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K

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x
A

L

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M

M

F

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P

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L

-

E

E

E

Y

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E

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A

binding iron/sulfur cluster: P678 (= P49), W680 (≠ F51), C685 (= C56), I686 (= I57), C688 (= C59), N689 (≠ D60), C691 (= C62), C695 (= C66), P696 (= P67), I700 (vs. gap), I734 (≠ V88), C741 (= C95), T742 (≠ K96), G743 (= G97), C744 (= C98), G745 (≠ A99), C747 (= C101), C751 (= C105), A757 (≠ S111)

Sites not aligning to the query:

active site: 28, 61, 111, 999
binding coenzyme a: 28, 423, 424, 427, 428, 553, 586, 999
binding magnesium ion: 966, 994, 996
binding iron/sulfur cluster: 807, 808, 811, 813, 836, 998, 1074, 1075
binding thiamine diphosphate: 26, 27, 61, 85, 813, 835, 836, 865, 965, 966, 967, 996, 997, 998, 999, 1000

6cipA Pyruvate:ferredoxin oxidoreductase from moorella thermoacetica with acetyl-tpp bound (see paper)
27% identity, 60% coverage: 46:124/132 of query aligns to 675:772/1165 of 6cipA

query
sites
6cipA

V

V

E

N

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P

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Q

F
x
W

N

Q

R

P

E

E

L

N

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C

I
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I

D

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C

D
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N

F

Q

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C

W

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S

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C

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P

D

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A

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x
I

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R

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P

-

Y

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L

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A

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K

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A

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D

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L

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A

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C

K
x
T

G

G

C
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C

A
x
G

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N

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C

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A

E

D

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V

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C

P

P

T

A

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x
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L

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T

M

M

F

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P

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L

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E

E

E

Y

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M

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D

A

N

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E

E

A

A

binding iron/sulfur cluster: P678 (= P49), W680 (≠ F51), C685 (= C56), I686 (= I57), C688 (= C59), N689 (≠ D60), C691 (= C62), C695 (= C66), A699 (≠ C70), I700 (vs. gap), I734 (≠ V88), C741 (= C95), T742 (≠ K96), C744 (= C98), G745 (≠ A99), C747 (= C101), C751 (= C105), A757 (≠ S111), L758 (= L112)

Sites not aligning to the query:

active site: 28, 61, 111, 999
binding 2-acetyl-thiamine diphosphate: 27, 61, 85, 111, 813, 834, 835, 836, 865, 965, 966, 967, 996, 998, 999
binding magnesium ion: 966, 994, 996
binding iron/sulfur cluster: 455, 807, 808, 811, 813, 836, 998, 1074

6cioA Pyruvate:ferredoxin oxidoreductase from moorella thermoacetica with lactyl-tpp bound (see paper)
27% identity, 60% coverage: 46:124/132 of query aligns to 675:772/1164 of 6cioA

query
sites
6cioA

V

V

E

N

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P

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x
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I

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C

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C

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C

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C
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A

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x
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A

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N

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x
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C

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D

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C

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L

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M

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A

binding iron/sulfur cluster: P678 (= P49), W680 (≠ F51), C685 (= C56), I686 (= I57), C688 (= C59), N689 (≠ D60), C691 (= C62), C695 (= C66), A699 (≠ C70), I700 (vs. gap), I734 (≠ V88), C741 (= C95), T742 (≠ K96), C744 (= C98), G745 (≠ A99), C747 (= C101), C751 (= C105), A757 (≠ S111), L758 (= L112)

Sites not aligning to the query:

active site: 28, 61, 111, 999
binding magnesium ion: 966, 994, 996
binding iron/sulfur cluster: 455, 807, 808, 811, 813, 836, 1074, 1075
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-carboxy-1-hydroxyethyl)-5-(2-{[hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: 26, 27, 28, 61, 85, 111, 813, 834, 835, 836, 865, 965, 966, 967, 996, 997, 998, 999, 1000

6cinB Crystal structure of pyruvate:ferredoxin oxidoreductase from moorella thermoacetica (see paper)
27% identity, 60% coverage: 46:124/132 of query aligns to 675:772/1169 of 6cinB

query
sites
6cinB

V

V

E

N

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P

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F

W

N

Q

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E

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N

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C

I
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I

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x
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D
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N

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C

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C

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I

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C

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x
G

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x
N

C
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C

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C

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L

L

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M

M

F

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L

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E

E

E

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V

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V

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E

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A

binding iron/sulfur cluster: P678 (= P49), C685 (= C56), I686 (= I57), Q687 (≠ D58), C688 (= C59), N689 (≠ D60), C691 (= C62), C695 (= C66), I700 (vs. gap), C741 (= C95), T742 (≠ K96), G743 (= G97), C744 (= C98), G745 (≠ A99), N746 (≠ V100), C747 (= C101), C751 (= C105), P752 (= P106), A757 (≠ S111), L758 (= L112)

Sites not aligning to the query:

active site: 28, 61, 111, 999
binding magnesium ion: 966, 994, 996
binding iron/sulfur cluster: 455, 807, 808, 811, 813, 836, 998, 1074, 1075
binding thiamine diphosphate: 26, 27, 61, 834, 835, 836, 865, 965, 966, 967, 996, 997, 998, 999, 1000

Q2RMD6 Pyruvate:ferredoxin oxidoreductase; PFOR; Pyruvate synthase; EC 1.2.7.1 from Moorella thermoacetica (strain ATCC 39073 / JCM 9320) (see paper)
27% identity, 60% coverage: 46:124/132 of query aligns to 676:773/1171 of Q2RMD6

query
sites
Q2RMD6

V

V

E

N

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P

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F

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Q

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E

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N

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C

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C
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C

D

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C

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C

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G

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C

A

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N

C
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C

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D

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C

P

P

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M

M

F

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E

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Y

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A

N

V

E

E

A

A

C686 (= C56) binding [4Fe-4S] cluster
C689 (= C59) binding [4Fe-4S] cluster
C692 (= C62) binding [4Fe-4S] cluster
C696 (= C66) binding [4Fe-4S] cluster
C742 (= C95) binding [4Fe-4S] cluster
C745 (= C98) binding [4Fe-4S] cluster
C748 (= C101) binding [4Fe-4S] cluster
C752 (= C105) binding [4Fe-4S] cluster

Sites not aligning to the query:

424:428 binding CoA
456 binding CoA
556 binding CoA
598 binding CoA
809 binding [4Fe-4S] cluster
812 binding [4Fe-4S] cluster
814 binding thiamine diphosphate
837 binding [4Fe-4S] cluster; binding thiamine diphosphate
967 binding Mg(2+)
967:969 binding thiamine diphosphate
995 binding Mg(2+)
995:1000 binding thiamine diphosphate
997 binding Mg(2+)
1075 binding [4Fe-4S] cluster

4hea9 Crystal structure of the entire respiratory complex i from thermus thermophilus (see paper)
33% identity, 60% coverage: 54:132/132 of query aligns to 50:134/180 of 4hea9

query
sites
4hea9

E

E

L

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C
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C

I

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D
x
G

C
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C

D
x
S

F
x
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C

W

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A

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C

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P

D

A

S

Y

C

A

F
x
I

L

Y

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E

E

E

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V

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V

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N

N

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P

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P

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A

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G

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K

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Y

A

A

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K

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I

I

N

N

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M

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C

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x
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x
F

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C

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x
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C

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E

E

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A

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C

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T

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x
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x
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L

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F

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M

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D

N

Y

E

E

E

Y

A

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K

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D

binding iron/sulfur cluster: C52 (= C56), G54 (≠ D58), C55 (= C59), S56 (≠ D60), L57 (≠ F61), C58 (= C62), C62 (= C66), P63 (= P67), I67 (≠ F71), Y90 (≠ V88), C97 (= C95), I98 (≠ K96), F99 (≠ G97), C100 (= C98), G101 (≠ A99), L102 (≠ V100), C103 (= C101), C107 (= C105), P108 (= P106), T109 (= T107), A111 (≠ I109), I112 (≠ K110)

Sites not aligning to the query:

binding iron/sulfur cluster: 40

Q56224 NADH-quinone oxidoreductase subunit 9; NADH dehydrogenase I subunit 9; NDH-1 subunit 9; EC 7.1.1.- from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
33% identity, 60% coverage: 54:132/132 of query aligns to 51:135/182 of Q56224

query
sites
Q56224

E

E

L

K

C
|
C

I

I

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C
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C

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x
S

F

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C
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C

W

A

V

A

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A

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C

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P

D

A

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Y

C

A

F

I

L

Y

V

V

E

E

E

P

V

A

V

E

N

N

-

D

-

P

-

E

-

N

-

P

-

V

K

S

R

A

G

G

K

E

K

R

Q

Y

A

A

K

K

I

V

V

Y

G

E

I

I

N

N

Y

M

N

L

L

R

C
|
C

K
x
I

G

F

C
|
C

A

G

V

L

C
|
C

V

E

E

E

V

A

C
|
C

P

P

T

T

P

G

I

A

K

I

S

V

L

L

L

G

M

Y

F

D

P

F

E

E

Y

M

M

A

D

D

N

Y

E

E

E

Y

A

S

L

D

K

L

Q

V

W

Y

P

G

K

K

K

E

D

D

C53 (= C56) binding [4Fe-4S] cluster
C56 (= C59) binding [4Fe-4S] cluster
S57 (≠ D60) binding [4Fe-4S] cluster
C59 (= C62) binding [4Fe-4S] cluster
C63 (= C66) binding [4Fe-4S] cluster
C98 (= C95) binding [4Fe-4S] cluster
I99 (≠ K96) binding [4Fe-4S] cluster
C101 (= C98) binding [4Fe-4S] cluster
C104 (= C101) binding [4Fe-4S] cluster
C108 (= C105) binding [4Fe-4S] cluster

8iufS8 ndufv2 (see paper)
32% identity, 40% coverage: 54:106/132 of query aligns to 73:125/182 of 8iufS8

query
sites
8iufS8

E

E

L

R

C
|
C

I
|
I

D
x
T

C
|
C

D
x
Q

F

L

C
|
C

W

E

V

V

S

T

C
|
C

P
|
P

D

A

S

Q

C

A

F
x
I

L

A

V

I

E

D

E

G

V

V

V

E

N

D

K

D

R

D

G

G

K

S

K

R

Q

M

A

A

K

S

I

R

V

F

G

D

I

L

N

D

Y

M

N

H

L

K

C
|
C

K
x
I

G
x
Y

C
|
C

A
x
G

V

L

C
|
C

V

Q

E

E

V

A

C
|
C

P

P

binding iron/sulfur cluster: C75 (= C56), I76 (= I57), T77 (≠ D58), C78 (= C59), Q79 (≠ D60), C81 (= C62), C85 (= C66), P86 (= P67), I90 (≠ F71), C114 (= C95), I115 (≠ K96), Y116 (≠ G97), C117 (= C98), G118 (≠ A99), C120 (= C101), C124 (= C105)

Sites not aligning to the query:

binding iron/sulfur cluster: 63, 129, 131

Query Sequence

>WP_007475071.1 NCBI__GCF_000170735.1:WP_007475071.1
MATPKEMLTTWDQVQFGAVLPAFEDPEHKKRSKFHSYNYTVADWRVEKPVFNRELCIDCD
FCWVSCPDSCFLVEEVVNKRGKKQAKIVGINYNLCKGCAVCVEVCPTPIKSLLMFPEYMD
NEEALKQWPKKD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory