SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_007508609.1 NCBI__GCF_000230695.2:WP_007508609.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0AAH0 Phosphate import ATP-binding protein PstB; ABC phosphate transporter; Phosphate-transporting ATPase; EC 7.3.2.1 from Escherichia coli (strain K12) (see paper)
67% identity, 94% coverage: 17:271/271 of query aligns to 2:257/257 of P0AAH0

query
sites
P0AAH0

A

S

A

M

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V

A

E

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T

A

A

P

P

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S

K

K

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A

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G

N

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I

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E

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K

N

Q

Q

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T

A

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G

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P

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S

G

G

C

C

G
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G

K
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K

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T

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F

N

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A

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A

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E

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D

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A

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K

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G

M

M

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F

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Q

K

K

P

P

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P

F

F

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M

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Y

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A

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D

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G

G48 (= G62) mutation to I: Loss of phosphate transport.
K49 (= K63) mutation to Q: Loss of phosphate transport.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
36% identity, 90% coverage: 23:266/271 of query aligns to 1:236/241 of 4u00A

query
sites
4u00A

P

P

K

I

L

I

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R

V

I

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R

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N

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L

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H

F

K

Y

W

Y
x
F

D

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A
x
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G

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S

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G

C
x
S

G
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G

K
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K

S
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S

T
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T

L

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N

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E

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M

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F

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L

binding adenosine-5'-diphosphate: F12 (≠ Y34), V17 (≠ A39), S37 (= S59), G38 (= G60), S39 (≠ C61), G40 (= G62), K41 (= K63), S42 (= S64), T43 (= T65)

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
38% identity, 90% coverage: 25:267/271 of query aligns to 2:237/240 of 4ymuJ

query
sites
4ymuJ

L

I

V

F

V

V

R

N

D

D

L

V

H

Y

F

K

Y

N

Y
x
F

D

G

G

S

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L

Q

E

A
x
V

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L

K

K

G

G

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T

M

L

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K

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N

E

K

K

G

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E

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V

T

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A

V

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I

G

G

P

P

S
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S

G
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G

C
x
S

G
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G

K
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K

S
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S

T
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T

L

L

R

R

I

C

F

I

N

N

R

L

I

L

Y

E

A

E

I

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Y

-

P

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L

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P

A

T

K

K

G

G

S

E

I

V

E

F

L

I

D

D

G

G

D

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N

K

I

I

L

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D

N

P

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G

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K

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G

M

M

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F

F

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Q

K

H

P

F

V

N

P

L

F

F

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P

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M

L

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T

I

A

F

I

E

E

N

N

V

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L

G

A

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L

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G

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M

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K

K

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T

S

binding adenosine-5'-triphosphate: F11 (≠ Y34), V16 (≠ A39), S36 (= S59), G37 (= G60), S38 (≠ C61), G39 (= G62), K40 (= K63), S41 (= S64), T42 (= T65), E162 (= E193), H194 (= H224)
binding magnesium ion: S41 (= S64), E162 (= E193)

3c4jA Abc protein artp in complex with atp-gamma-s
37% identity, 90% coverage: 27:270/271 of query aligns to 6:242/242 of 3c4jA

query
sites
3c4jA

V

V

R

H

D

Q

L

L

H

K

F

K

Y

S

Y
x
F

D

G

G

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K

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K

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S

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T

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F

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K

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D

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G

D

I

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N

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G

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M

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F

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Q

K

R

P

F

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N

P

L

F

F

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H

M

M

T

T

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F

L

E

N

N

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A

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D

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K

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D

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K

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A

N

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A

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G

Q

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A

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C

A

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I

A

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A

A

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A

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S

A

A

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E

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E

L

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K

E

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A

S

N

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E

-

G

F

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M

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T

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H

N

E

M

M

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Q

F

A

A

A

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G

D

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A

L

F

F

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M

Y

D

L

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G

G

E

Y

L

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I

I

E

E

F

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G

Q

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E

E

R

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I

L

F

F

T

D

K

R

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P

G

Q

K

H

K

E

Q

R

T

T

E

K

D

A

Y

F

I

L

T

S

G

K

R

V

F

F

binding phosphothiophosphoric acid-adenylate ester: F13 (≠ Y34), V18 (≠ A39), S38 (= S59), G39 (= G60), S40 (≠ C61), G41 (= G62), K42 (= K63), S43 (= S64), T44 (= T65)

3c41J Abc protein artp in complex with amp-pnp/mg2+
37% identity, 90% coverage: 27:270/271 of query aligns to 6:242/242 of 3c41J

query
sites
3c41J

V

V

R

H

D

Q

L

L

H

K

F

K

Y

S

Y
x
F

D

G

G

S

F

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Q

E

A
x
V

L

L

K

K

G

G

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H

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E

K

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G

P

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S
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S

G
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G

C

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G
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G

K
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K

S
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S

T
|
T

L

F

L

L

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R

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C

F

L

N

N

R

L

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Y

E

A

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Y

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P

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K

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L

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-

A

D

K

E

G

G

S

E

I

I

E

I

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I

D

D

G

G

D

I

N

N

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K

D

A

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K

G

D

Y

T

S

N

L

L

N

N

R

K

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E

K

E

V

V

G

G

M

M

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V

F

F

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Q

K

R

P

F

V

N

P

L

F

F

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P

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M

M

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T

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F

L

E

N

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K

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D
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D

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A

A

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E

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H

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A

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K

R

V

F

F

binding phosphoaminophosphonic acid-adenylate ester: F13 (≠ Y34), V18 (≠ A39), S38 (= S59), G39 (= G60), G41 (= G62), K42 (= K63), S43 (= S64), T44 (= T65)
binding magnesium ion: S43 (= S64), D163 (= D192)

2olkA Abc protein artp in complex with adp-beta-s
37% identity, 90% coverage: 27:270/271 of query aligns to 6:242/242 of 2olkA

query
sites
2olkA

V

V

R

H

D

Q

L

L

H

K

F

K

Y

S

Y
x
F

D

G

G

S

F

L

Q

E

A
x
V

L

L

K

K

G

G

I

I

S

N

M

V

D

H

I

I

P

R

E

E

K

G

Q

E

V

V

T

V

A

V

I

V

I

I

G

G

P

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

R

R

I

C

F

L

N

N

R

L

I

L

Y

E

A

D

I

F

Y

-

P

-

K

-

L

-

E

-

A

D

K

E

G

G

S

E

I

I

E

I

L

I

D

D

G

G

D

I

N

N

I

L

L

K

D

A

P

K

G

D

Y

T

S

N

L

L

N

N

R

K

L

V

R

R

S

E

K

E

V

V

G

G

M

M

V

V

F

F

Q

Q

K

R

P

F

V

N

P

L

F

F

P

P

-

H

M

M

T

T

I

V

F

L

E

N

N

N

V

I

A

T

Y

L

G

A

I

P

R

M

H

K

H

V

E

R

K

K

L

W

S

P

K

R

A

E

D

K

M

A

E

E

G

A

R

K

V

A

E

M

Q

E

A

L

L

L

R

D

S

K

A

V

A

G

L

L

W

-

D

-

E

-

V

-

K

K

D

D

K

K

L

A

R

H

Q

A

N

Y

A

P

L

D

G

S

L

L

S

S

G

G

Q

Q

Q

A

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

I

L

A

A

L

M

R

E

P

P

E

K

V

I

L

M

L

L

L

F

D

D

E

E

P

P

T

T

S

S

A

A

L

L

D

D

P

P

I

E

A

M

T

V

S

G

R

E

I

V

E

L

Q

S

L

V

V

M

E

K

E

Q

L

L

K

A

S

N

Q

E

-

G

F

M

T

T

I

M

V

V

I

V

V

V

T

T

H

H

N

E

M

M

Q

G

Q

F

A

A

A

R

R

E

V

V

S

G

D

D

L

R

T

V

A

L

F

F

M

M

Y

D

L

G

G

G

E

Y

L

I

I

I

E

E

F

E

D

G

Q

K

T

P

E

E

R

D

I

L

F

F

T

D

K

R

P

P

G

Q

K

H

K

E

Q

R

T

T

E

K

D

A

Y

F

I

L

T

S

G

K

R

V

F

F

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (≠ Y34), V18 (≠ A39), S38 (= S59), G39 (= G60), S40 (≠ C61), G41 (= G62), K42 (= K63), S43 (= S64), T44 (= T65)

2oljA Abc protein artp in complex with adp/mg2+
37% identity, 90% coverage: 27:270/271 of query aligns to 6:242/242 of 2oljA

query
sites
2oljA

V

V

R

H

D

Q

L

L

H

K

F

K

Y

S

Y
x
F

D

G

G

S

F

L

Q

E

A
x
V

L

L

K

K

G

G

I

I

S

N

M

V

D

H

I

I

P

R

E

E

K

G

Q

E

V

V

T

V

A

V

I

V

I

I

G

G

P

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

R

R

I

C

F

L

N

N

R

L

I

L

Y

E

A

D

I

F

Y

-

P

-

K

-

L

-

E

-

A

D

K

E

G

G

S

E

I

I

E

I

L

I

D

D

G

G

D

I

N

N

I

L

L

K

D

A

P

K

G

D

Y

T

S

N

L

L

N

N

R

K

L

V

R

R

S

E

K

E

V

V

G

G

M

M

V

V

F

F

Q

Q

K

R

P

F

V

N

P

L

F

F

P

P

-

H

M

M

T

T

I

V

F

L

E

N

N

N

V

I

A

T

Y

L

G

A

I

P

R

M

H

K

H

V

E

R

K

K

L

W

S

P

K

R

A

E

D

K

M

A

E

E

G

A

R

K

V

A

E

M

Q

E

A

L

L

L

R

D

S

K

A

V

A

G

L

L

W

-

D

-

E

-

V

-

K

K

D

D

K

K

L

A

R

H

Q

A

N

Y

A

P

L

D

G

S

L

L

S

S

G

G

Q

Q

Q

A

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

I

L

A

A

L

M

R

E

P

P

E

K

V

I

L

M

L

L

L

F

D

D

E

E

P

P

T

T

S

S

A

A

L

L

D

D

P

P

I

E

A

M

T

V

S

G

R

E

I

V

E

L

Q

S

L

V

V

M

E

K

E

Q

L

L

K

A

S

N

Q

E

-

G

F

M

T

T

I

M

V

V

I

V

V

V

T

T

H

H

N

E

M

M

Q

G

Q

F

A

A

A

R

R

E

V

V

S

G

D

D

L

R

T

V

A

L

F

F

M

M

Y

D

L

G

G

G

E

Y

L

I

I

I

E

E

F

E

D

G

Q

K

T

P

E

E

R

D

I

L

F

F

T

D

K

R

P

P

G

Q

K

H

K

E

Q

R

T

T

E

K

D

A

Y

F

I

L

T

S

G

K

R

V

F

F

binding adenosine-5'-diphosphate: F13 (≠ Y34), V18 (≠ A39), S38 (= S59), G39 (= G60), S40 (≠ C61), G41 (= G62), K42 (= K63), S43 (= S64), T44 (= T65)

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
36% identity, 85% coverage: 37:266/271 of query aligns to 18:241/343 of P30750

query
sites
P30750

F

I

Q

Q

A

A

L

L

K

N

G

N

I

V

S

S

M

L

D

H

I

V

P

P

E

A

K

G

Q

Q

V

I

T

Y

A

G

I

V

I

I

G

G

P

A

S
|
S

G
|
G

C
x
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

R

R

I

C

F

V

N

N

R

L

I

L

Y

-

A

-

I

-

Y

-

P

-

K

E

L

R

E

P

A

T

K

E

G

G

S

S

I

V

E

L

L

V

D

D

G

G

D

Q

N

E

I

L

L

T

D

T

P

L

G

S

Y

E

S

S

-

E

L

L

N

T

R

K

L

A

R

R

S

R

K

Q

V

I

G

G

M

M

V

I

F

F

Q

Q

K

H

-

F

P

N

V

L

P

L

F

S

P

S

M

R

T

T

I

V

F

F

E

G

N

N

V

V

A

A

Y

L

G

P

I

L

R

E

H

L

H

-

E

D

K

N

L

T

S

P

K

K

A

D

D

E

M

V

E

K

G

R

R

R

V

V

E

T

Q

E

A

L

L

L

R

S

S

L

A

V

A

G

L

L

W

G

D

D

E

K

V

-

K

H

D

D

K

S

L

Y

R

P

Q

S

N

N

A

-

L

-

G

-

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

I

L

A

A

L

S

R

N

P

P

E

K

V

V

L

L

L

C

D

D

E
|
E

P

A

T

T

S

S

A

A

L

L

D

D

P

P

I

A

A

T

T

T

S

R

R

S

I

I

E

L

Q

E

L

L

V

L

E

K

E

D

L

I

K

N

S

R

Q

R

-

L

-

G

F

L

T

T

I

I

V

L

I

L

V

I

T

T

H

H

N

E

M

M

Q

D

Q

V

A

V

A

K

R

R

V

I

S

C

D

D

L

C

T

V

A

A

F

V

M

I

Y

S

L

N

G

G

E

E

L

L

I

I

E

E

F

Q

D

D

Q

T

T

V

E

S

R

E

I

V

F

F

T

S

K

H

P

P

G

K

K

T

K

P

Q

L

T

A

E

Q

D

K

Y

F

I

I

40:46 (vs. 59:65, 86% identical) binding ATP
E166 (= E193) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding L-methionine
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding L-methionine

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
36% identity, 85% coverage: 37:266/271 of query aligns to 19:242/344 of 3tuzC

query
sites
3tuzC

F

I

Q

Q

A

A

L

L

K

N

G

N

I

V

S

S

M

L

D

H

I

V

P

P

E

A

K

G

Q

Q

V

I

T

Y

A

G

I

V

I

I

G

G

P

A

S

S

G
|
G

C

A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

R

R

I

C

F

V

N

N

R

L

I

L

Y

-

A

-

I

-

Y

-

P

-

K

E

L

R

E

P

A

T

K

E

G

G

S

S

I

V

E

L

L

V

D

D

G

G

D

Q

N

E

I

L

L

T

D

T

P

L

G

S

Y

E

S

S

-

E

L

L

N

T

R

K

L

A

R

R

S

R

K

Q

V

I

G

G

M

M

V

I

F

F

Q

Q

K

H

-

F

P

N

V

L

P

L

F

S

P

S

M

R

T

T

I

V

F

F

E

G

N

N

V

V

A

A

Y

L

G

P

I

L

R

E

H

L

H

-

E

D

K

N

L

T

S

P

K

K

A

D

D

E

M

V

E

K

G

R

R

R

V

V

E

T

Q

E

A

L

L

L

R

S

S

L

A

V

A

G

L

L

W

G

D

D

E

K

V

-

K

H

D

D

K

S

L

Y

R

P

Q

S

N

N

A

-

L

-

G

-

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

I

L

A

A

L

S

R

N

P

P

E

K

V

V

L

L

L

C

D

D

E

Q

P

A

T

T

S

S

A

A

L

L

D

D

P

P

I

A

A

T

T

T

S

R

R

S

I

I

E

L

Q

E

L

L

V

L

E

K

E

D

L

I

K

N

S

R

Q

R

-

L

-

G

F

L

T

T

I

I

V

L

I

L

V

I

T

T

H

H

N

E

M

M

Q

D

Q

V

A

V

A

K

R

R

V

I

S

C

D

D

L

C

T

V

A

A

F

V

M

I

Y

S

L

N

G

G

E

E

L

L

I

I

E

E

F

Q

D

D

Q

T

T

V

E

S

R

E

I

V

F

F

T

S

K

H

P

P

G

K

K

T

K

P

Q

L

T

A

E

Q

D

K

Y

F

I

I

binding adenosine-5'-diphosphate: G42 (= G60), G44 (= G62), K45 (= K63), S46 (= S64), T47 (= T65)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12, 14
binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
36% identity, 85% coverage: 37:266/271 of query aligns to 19:242/344 of 3tuiC

query
sites
3tuiC

F

I

Q

Q

A

A

L

L

K

N

G

N

I

V

S

S

M

L

D

H

I

V

P

P

E

A

K

G

Q

Q

V

I

T

Y

A

G

I

V

I

I

G

G

P

A

S

S

G
|
G

C
x
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

R

R

I

C

F

V

N

N

R

L

I

L

Y

-

A

-

I

-

Y

-

P

-

K

E

L

R

E

P

A

T

K

E

G

G

S

S

I

V

E

L

L

V

D

D

G

G

D

Q

N

E

I

L

L

T

D

T

P

L

G

S

Y

E

S

S

-

E

L

L

N

T

R

K

L

A

R

R

S

R

K

Q

V

I

G

G

M

M

V

I

F

F

Q

Q

K

H

-

F

P

N

V

L

P

L

F

S

P

S

M

R

T

T

I

V

F

F

E

G

N

N

V

V

A

A

Y

L

G

P

I

L

R

E

H

L

H

-

E

D

K

N

L

T

S

P

K

K

A

D

D

E

M

V

E

K

G

R

R

R

V

V

E

T

Q

E

A

L

L

L

R

S

S

L

A

V

A

G

L

L

W

G

D

D

E

K

V

-

K

H

D

D

K

S

L

Y

R

P

Q

S

N

N

A

-

L

-

G

-

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

I

L

A

A

L

S

R

N

P

P

E

K

V

V

L

L

L

C

D

D

E

Q

P

A

T

T

S

S

A

A

L

L

D

D

P

P

I

A

A

T

T

T

S

R

R

S

I

I

E

L

Q

E

L

L

V

L

E

K

E

D

L

I

K

N

S

R

Q

R

-

L

-

G

F

L

T

T

I

I

V

L

I

L

V

I

T

T

H

H

N

E

M

M

Q

D

Q

V

A

V

A

K

R

R

V

I

S

C

D

D

L

C

T

V

A

A

F

V

M

I

Y

S

L

N

G

G

E

E

L

L

I

I

E

E

F

Q

D

D

Q

T

T

V

E

S

R

E

I

V

F

F

T

S

K

H

P

P

G

K

K

T

K

P

Q

L

T

A

E

Q

D

K

Y

F

I

I

binding adenosine-5'-diphosphate: G42 (= G60), A43 (≠ C61), G44 (= G62), K45 (= K63), S46 (= S64), T47 (= T65)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
36% identity, 85% coverage: 37:266/271 of query aligns to 19:242/344 of 6cvlD

query
sites
6cvlD

F
x
I

Q

Q

A

A

L

L

K

N

G

N

I

V

S

S

M

L

D

H

I

V

P

P

E

A

K

G

Q

Q

V

I

T

Y

A

G

I

V

I

I

G

G

P

A

S
|
S

G
|
G

C
x
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

R

R

I

C

F

V

N

N

R

L

I

L

Y

-

A

-

I

-

Y

-

P

-

K

E

L

R

E

P

A

T

K

E

G

G

S

S

I

V

E

L

L

V

D

D

G

G

D

Q

N

E

I

L

L

T

D

T

P

L

G

S

Y

E

S

S

-

E

L

L

N

T

R

K

L

A

R

R

S

R

K

Q

V

I

G

G

M

M

V

I

F

F

Q

Q

K

H

-

F

P

N

V

L

P

L

F

S

P

S

M

R

T

T

I

V

F

F

E

G

N

N

V

V

A

A

Y

L

G

P

I

L

R

E

H

L

H

-

E

D

K

N

L

T

S

P

K

K

A

D

D

E

M

V

E

K

G

R

R

R

V

V

E

T

Q

E

A

L

L

L

R

S

S

L

A

V

A

G

L

L

W

G

D

D

E

K

V

-

K

H

D

D

K

S

L

Y

R

P

Q

S

N
|
N

A

-

L

-

G

-

L

L

S
|
S

G

G

Q
|
Q

Q

K

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

I

L

A

A

L

S

R

N

P

P

E

K

V

V

L

L

L

C

D

D

E

Q

P

A

T

T

S

S

A

A

L

L

D

D

P

P

I

A

A

T

T

T

S

R

R

S

I

I

E

L

Q

E

L

L

V

L

E

K

E

D

L

I

K

N

S

R

Q

R

-

L

-

G

F

L

T

T

I

I

V

L

I

L

V

I

T

T

H
|
H

N

E

M

M

Q

D

Q

V

A

V

A

K

R

R

V

I

S

C

D

D

L

C

T

V

A

A

F

V

M

I

Y

S

L

N

G

G

E

E

L

L

I

I

E

E

F

Q

D

D

Q

T

T

V

E

S

R

E

I

V

F

F

T

S

K

H

P

P

G

K

K

T

K

P

Q

L

T

A

E

Q

D

K

Y

F

I

I

binding phosphothiophosphoric acid-adenylate ester: I19 (≠ F37), S41 (= S59), G42 (= G60), A43 (≠ C61), G44 (= G62), K45 (= K63), S46 (= S64), T47 (= T65), N141 (= N164), S143 (= S169), Q146 (= Q172), H200 (= H224)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 12, 14

7fc9A Crystal structure of cmabcb1 in lipidic mesophase revealed by lcp-sfx (see paper)
36% identity, 87% coverage: 10:246/271 of query aligns to 326:564/584 of 7fc9A

query
sites
7fc9A

P

P

T

D

P

P

G

G

A

A

A

E

A

V

A

T

V

Q

A

P

V

L

A

V

P

L

K

K

-

Q

-

G

L

I

V

V

V

F

R

E

D
x
N

L

V

H
|
H

F

F

Y

R

Y
|
Y

D

P

-

T

-
x
R

-

M

G

N

F

V

Q
x
E

A

V

L

L

K

R

G

G

I

I

S

S

M

L

D

T

I

I

P

P

E

N

K

G

Q

K

V

T

T

V

A

A

I

I

I

V

G

G

P

G

S
|
S

G

G

C

A

G
|
G

K
|
K

S
|
S

T
|
T

L

I

R

Q

I

L

F

L

N

M

R

R

I

F

Y

Y

A

D

I

I

Y

E

P

P

K

-

L

-

E

Q

A

G

K

G

G

G

S

L

I

L

E

L

L

F

D

D

G

G

D

T

N

P

I

A

L

W

D

N

P

-

G

-

Y

Y

S

D

L

F

N

H

R

A

L

L

R

R

S

S

K

Q

V

I

G

G

M

L

V

V

F

S

Q
|
Q

K

E

P

P

V

V

P

L

F

F

P

S

M

G

T

T

I

I

F

R

E

D

N

N

V

I

A

L

Y

Y

G

G

I

K

R

R

H

D

H

-

E

-

K

-

L

-

S

-

K

A

A

T

D
|
D

M
x
E

E

E

G

-

R

-

V

V

E

I

Q

Q

A

A

L

L

R

R

S

E

A

A

-

N

-

A

-

Y

-

S

-

F

-

V

-

M

A

A

L

L

W

P

D

D

E

G

V

L

K

D

D

T

K

E

L

V

R

G

Q

E

N

R

A

G

L

L

G

A

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

I

C

A

I

I

A

A

R

R

A

A

I

I

A

L

L

K

R

H

P

P

E

T

V

L

L

L

L

C

L

L

D

D

E
|
E

P

S

T

T

S

S

A

A

L

L

D

D

P

A

I

E

A

S

T

E

S

A

R

L

I

V

E

Q

Q

E

L

A

V

L

E

D

E

R

L

M

K

M

S

A

Q

S

-

D

-

G

F

V

T

T

I

S

V

V

I

V

V

I

T

A

H
|
H

N

R

M

L

Q

S

Q

T

A

V

A

A

R

R

V

-

S

A

D

D

L

L

T

I

A

L

F

V

M

M

Y

Q

L

D

G

G

E

V

L

V

I

V

E

E

binding phosphoaminophosphonic acid-adenylate ester: Y352 (= Y34), R355 (vs. gap), S380 (= S59), G383 (= G62), K384 (= K63), S385 (= S64), T386 (= T65), Q429 (= Q112), H543 (= H224)
binding magnesium ion: S385 (= S64), Q429 (= Q112)
binding zinc ion: N347 (≠ D29), H349 (= H31), H349 (= H31), E359 (≠ Q38), D451 (= D139), E452 (≠ M140), E510 (= E193), H543 (= H224)

Sites not aligning to the query:

binding magnesium ion: 127, 188, 192, 312
binding zinc ion: 31, 74, 119, 127, 192, 193

3wmgA Crystal structure of an inward-facing eukaryotic abc multidrug transporter g277v/a278v/a279v mutant in complex with an cyclic peptide inhibitor, acap (see paper)
36% identity, 87% coverage: 10:246/271 of query aligns to 325:563/589 of 3wmgA

query
sites
3wmgA

P

P

T

D

P

P

G

G

A

A

A

E

A

V

A

T

V

Q

A

P

V

L

A

V

P

L

K

K

-

Q

-

G

L

I

V

V

V

F

R

E

D

N

L

V

H

H

F

F

Y

R

Y

Y

D

P

-

T

-

R

-

M

G

N

F

V

Q

E

A

V

L

L

K

R

G

G

I

I

S

S

M

L

D

T

I

I

P

P

E

N

K

G

Q

K

V

T

T

V

A

A

I

I

I

V

G

G

P

G

S

S

G

G

C

A

G

G

K

K

S

S

T

T

L

I

R

Q

I

L

F

L

N

M

R

R

I

F

Y

Y

A

D

I

I

Y

E

P

P

K

-

L

-

E

Q

A

G

K

G

G

G

S

L

I

L

E

L

L

F

D

D

G

G

D

T

N

P

I

A

L

W

D

N

P

-

G

-

Y

Y

S

D

L

F

N

H

R

A

L

L

R

R

S

S

K

Q

V

I

G

G

M

L

V

V

F

S

Q

Q

K

E

P

P

V

V

P

L

F

F

P

S

M

G

T

T

I

I

F

R

E

D

N

N

V

I

A

L

Y

Y

G

G

I

K

R

R

H

D

H

-

E

-

K

-

L

-

S

-

K

A

A

T

D

D

M

E

E

E

G

-

R

-

V

V

E

I

Q

Q

A

A

L

L

R

R

S

E

A

A

-

N

-

A

-

Y

-

S

-

F

-

V

-

M

A

A

L

L

W

P

D

D

E

G

V

L

K

D

D

T

K

E

L

V

R

G

Q

E

N

R

A

G

L

L

G

A

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

I

C

A

I

I

A

A

R

R

A

A

I

I

A

L

L

K

R

H

P

P

E

T

V

L

L

L

L

C

L

L

D

D

E

E

P

S

T

T

S

S

A

A

L

L

D

D

P

A

I

E

A

S

T

E

S

A

R

L

I

V

E

Q

Q

E

L

A

V

L

E

D

E

R

L

M

K

M

S

A

Q

S

-

D

-

G

F

V

T

T

I

S

V

V

I

V

V

I

T

A

H

H

N

R

M

L

Q

S

Q

T

A

V

A

A

R

R

V

-

S

A

D

D

L

L

T

I

A

L

F

V

M

M

Y

Q

L

D

G

G

E

V

L

V

I

V

E

E

Sites not aligning to the query:

binding decyl-beta-d-maltopyranoside: 6, 13, 13, 18
binding : 43, 61, 62, 277

6a6mA Crystal structure of an outward-open nucleotide-bound state of the eukaryotic abc multidrug transporter cmabcb1 (see paper)
36% identity, 87% coverage: 10:246/271 of query aligns to 326:564/589 of 6a6mA

query
sites
6a6mA

P

P

T

D

P

P

G

G

A

A

A

E

A

V

A

T

V

Q

A

P

V

L

A

V

P

L

K

K

-

Q

-

G

L

I

V

V

V

F

R

E

D

N

L

V

H

H

F

F

Y

R

Y
|
Y

D

P

-

T

-
x
R

-

M

G

N

F

V

Q

E

A

V

L

L

K

R

G

G

I

I

S

S

M

L

D

T

I

I

P

P

E

N

K

G

Q

K

V

T

T

V

A

A

I

I

I

V

G
|
G

P
x
G

S
|
S

G
|
G

C

A

G
|
G

K
|
K

S
|
S

T
|
T

L

I

R

Q

I

L

F

L

N

M

R

R

I

F

Y

Y

A

D

I

I

Y

E

P

P

K

-

L

-

E

Q

A

G

K

G

G

G

S

L

I

L

E

L

L

F

D

D

G

G

D

T

N

P

I

A

L

W

D

N

P

-

G

-

Y

Y

S

D

L

F

N

H

R

A

L

L

R

R

S

S

K

Q

V

I

G

G

M

L

V

V

F

S

Q
|
Q

K

E

P

P

V

V

P

L

F

F

P

S

M

G

T

T

I

I

F

R

E

D

N

N

V

I

A

L

Y

Y

G

G

I

K

R

R

H

D

H

-

E

-

K

-

L

-

S

-

K

A

A

T

D

D

M

E

E

E

G

-

R

-

V

V

E

I

Q

Q

A

A

L

L

R

R

S

E

A

A

-

N

-

A

-

Y

-

S

-

F

-

V

-

M

A

A

L

L

W

P

D

D

E

G

V

L

K

D

D

T

K

E

L

V

R

G

Q

E

N

R

A

G

L

L

G

A

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

I

C

A

I

I

A

A

R

R

A

A

I

I

A

L

L

K

R

H

P

P

E

T

V

L

L

L

L

C

L

L

D

D

E

E

P

S

T

T

S

S

A

A

L

L

D

D

P

A

I

E

A

S

T

E

S

A

R

L

I

V

E

Q

Q

E

L

A

V

L

E

D

E

R

L

M

K

M

S

A

Q

S

-

D

-

G

F

V

T

T

I

S

V

V

I

V

V

I

T

A

H

H

N

R

M
x
L

Q

S

Q

T

A

V

A

A

R

R

V

-

S

A

D

D

L

L

T

I

A

L

F

V

M

M

Y
x
Q

L
x
D

G

G

E

V

L

V

I

V

E

E

binding phosphoaminophosphonic acid-adenylate ester: Y352 (= Y34), R355 (vs. gap), S380 (= S59), G383 (= G62), K384 (= K63), S385 (= S64), T386 (= T65), Q429 (= Q112)
binding decyl-beta-d-maltopyranoside: G378 (= G57), G379 (≠ P58), G381 (= G60), L545 (≠ M226), Q558 (≠ Y240), D559 (≠ L241)
binding magnesium ion: S385 (= S64), Q429 (= Q112)

Sites not aligning to the query:

binding decyl-beta-d-maltopyranoside: 579, 583

4ayxA Structure of the human mitochondrial abc transporter, abcb10 (rod form b) (see paper)
33% identity, 85% coverage: 28:257/271 of query aligns to 342:569/571 of 4ayxA

query
sites
4ayxA

R

K

D

N

L

V

H

H

F

F

Y

A

Y
|
Y

D

P

G

A

-

R

-

P

-

E

F

V

Q

P

A

I

L

F

K

Q

G

D

I

F

S

S

M

L

D

S

I

I

P

P

E

S

K

G

Q

S

V

V

T

T

A

A

I

L

I

V

G

G

P

P

S
|
S

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

L

V

L

L

R

S

I

L

F

L

N

L

R

R

I

L

Y

Y

A

D

I

-

Y

-

P

-

K

-

L

-

E

P

A

A

K

S

G

G

S

T

I

I

E

S

L

L

D

D

G

G

D

H

N

D

I

I

-

R

-

Q

L

L

D

N

P

P

G

V

Y

W

S

-

L

-

N

-

R

-

L

L

R

R

S

S

K

K

V

I

G

G

M

T

V

V

F

S

Q
|
Q

K

E

P

P

V

I

P

L

F

F

P

S

M

C

T

S

I

I

F

A

E

E

N

N

V

I

A

A

Y

Y

G

G

I

A

R

D

H

D

H

P

E

S

K

S

L

V

S

T

K

A

A

E

D

E

M

I

E

Q

G

-

R

R

V

V

E

A

Q

E

A

V

L

A

R

N

S

A

A

V

A

A

L

F

W

I

D

R

E

N

V

F

K

P

D

Q

K

G

L

F

R

N

-

T

-

V

-

G

Q

E

N

K

A

G

L

V

G

L

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

I

C

A

I

I

A

A

R

R

A

A

I

L

A

L

L

K

R

N

P

P

E

K

V

I

L

L

D

D

E

E

P

A

T

T

S

S

A

A

L

L

D

D

P

A

I

E

A

N

T

E

S

Y

R

L

I

V

E

Q

Q

E

L

A

V

L

E

D

E

R

L

L

K

M

S

D

Q

G

F

R

T

T

I

V

V

L

I

V

V

I

T

A

H

H

N

R

M

L

Q

-

Q

S

A

T

A

I

R

K

V

N

S

A

D

N

L

M

T

V

A

A

F

V

M

L

Y

D

L

Q

G

G

E

K

L

I

I

T

E

E

F

Y

D

G

Q

K

T

H

E

E

R

E

I

L

F

L

T

S

K

K

P

P

binding phosphomethylphosphonic acid adenylate ester: Y348 (= Y34), S376 (= S59), G377 (= G60), G379 (= G62), K380 (= K63), S381 (= S64), T382 (= T65)
binding magnesium ion: S381 (= S64), Q422 (= Q112)

Sites not aligning to the query:

binding glycine: 17, 81

Q9NRK6 ATP-binding cassette sub-family B member 10, mitochondrial; ABC-mitochondrial erythroid protein; ABC-me protein; ATP-binding cassette transporter 10; ABC transporter 10 protein; Mitochondrial ATP-binding cassette 2; M-ABC2 from Homo sapiens (Human) (see 5 papers)
33% identity, 86% coverage: 25:257/271 of query aligns to 492:722/738 of Q9NRK6

query
sites
Q9NRK6

L

L

V

E

V

F

R

K

D

N

L

V

H

H

F

F

Y

A

Y

Y

D

P

G

A

-

R

-

P

-

E

F

V

Q

P

A

I

L

F

K

Q

G

D

I

F

S

S

M

L

D

S

I

I

P

P

E

S

K

G

Q

S

V

V

T

T

A

A

I

L

I

V

G

G

P

P

S

S

G

G

C

S

G

G

K
|
K

S

S

T

T

L

V

L

L

R

S

I

L

F

L

N

L

R

R

I

L

Y

Y

A
x
D

I

-

Y

-

P

-

K

-

L

-

E

P

A

A

K

S

G

G

S

T

I

I

E

S

L

L

D

D

G

G

D

H

N

D

I

I

-

R

-

Q

L

L

D

N

P

P

G

V

Y

W

S

-

L

-

N

-

R

-

L

L

R

R

S

S

K

K

V

I

G

G

M

T

V

V

F

S

Q

Q

K

E

P

P

V

I

P

L

F

F

P

S

M
x
C

T

S

I

I

F

A

E

E

N

N

V

I

A

A

Y

Y

G

G

I

A

R

D

H

D

H

P

E

S

K

S

L

V

S

T

K

A

A

E

D

E

M

I

E

Q

G

-

R

R

V

V

E

A

Q

E

A

V

L

A

R

N

S

A

A

V

A

A

L

F

W

I

D

R

E

N

V

F

K

P

D

Q

K

G

L

F

R

N

-

T

-

V

-

G

Q

E

N

K

A

G

L

V

G

L

L

L

S
|
S

G

G

Q
|
Q

Q

K

Q

Q

R

R

L

I

C

A

I

I

A

A

R

R

A

A

I

L

A

L

L

K

R

N

P

P

E

K

V

I

L

L

D
|
D

E
|
E

P

A

T

T

S

S

A

A

L

L

D

D

P

A

I

E

A

N

T

E

S

Y

R

L

I

V

E

Q

Q

E

L

A

V

L

E

D

E

R

L

L

K

M

S

D

Q

G

F

R

T

T

I

V

V

L

I

V

V

I

T

A

H

H

N

R

M

L

Q

-

Q

S

A

T

A

I

R

K

V

N

S

A

D

N

L

M

T

V

A

A

F

V

M

L

Y

D

L

Q

G

G

E

K

L

I

I

T

E

E

F

Y

D

G

Q

K

T

H

E

E

R

E

I

L

F

L

T

S

K

K

P

P

K533 (= K63) mutation to E: Increases hemoglobin biosynthetic process.
D545 (≠ A75) to N: in dbSNP:rs35698797
C582 (≠ M119) mutation to G: Does not affect ATPase activity; when associated with S-215 and L-224. Activated by Zn (II) mesoporphyrin; when associated with S-215 and L-224.
S635 (= S169) mutation to R: Does not rescue hemoglobin and heme biosynthetic process.
Q638 (= Q172) mutation to H: Does not rescue hemoglobin and heme biosynthetic process.
D658 (= D192) mutation to A: Does not rescue hemoglobin and heme biosynthetic process.
E659 (= E193) mutation to A: Does not rescue hemoglobin and heme biosynthetic process.

Sites not aligning to the query:

150 A → S: in dbSNP:rs4148756
215 C→S: Does not affect ATPase activity; when associated with L-224 and G-582. Activated by Zn (II) mesoporphyrin; when associated with L-224 and G-582.
224 C→L: Does not affect ATPase activity; when associated withS-215 and G-582. Activated by Zn (II) mesoporphyrin; when associated with S-215 and G-582.
471 R → T: in a breast cancer sample; somatic mutation

7ahhC Opua inhibited inward-facing, sbd docked (see paper)
37% identity, 82% coverage: 44:266/271 of query aligns to 46:263/382 of 7ahhC

query
sites
7ahhC

S

N

M

F

D

E

I

I

P

N

E

E

K

G

Q

E

V

I

T

F

A

V

I

I

I

M

G

G

P

L

S

S

G
|
G

C

S

G
|
G

K
|
K

S

S

T

T

L

L

R

R

I

L

F

L

N

N

R

R

I

L

Y

-

A

-

I

I

Y

E

P

P

K

-

L

-

E

-

A

T

K

S

G

G

S

K

I

I

E

F

L

I

D

D

G

N

D

Q

N

D

I

V

-

A

-

T

L

L

D

N

P

K

G

E

Y

D

S

L

L

L

N

Q

R

V

L

R

R

R

S

K

K

T

V

M

G

S

M

M

V

V

F

F

Q

Q

K

N

P

F

V

G

P

L

F

F

P

P

M

H

-

R

T

T

I

I

F

L

E

E

N

N

V

T

A

E

Y

Y

G

G

I

L

R

-

H

E

H

V

E

Q

K

N

L

V

S

P

K

K

A

E

D

E

M

R

E

R

G

K

R

R

V

A

E

E

Q

K

A

A

L

L

R

D

S

N

A

A

A

N

L

L

W

L

D

D

E

-

V

F

K

K

D

D

K

Q

L

Y

R

P

Q

K

N

Q

A

-

L

-

G

-

L

L

S

S

G

G

Q

M

Q

Q

R

R

L

V

C

G

I

L

A

A

R

R

A

A

I

L

A

A

L

N

R

D

P

P

E

E

V

I

L

L

L

M

D
|
D

E
|
E

P

A

T

F

S

S

A

A

L

L

D

D

P

P

I

L

A

I

T

R

S

R

R

E

I

M

E

Q

Q

D

L

E

V

L

E

L

E

E

L

L

K

Q

S

A

Q

K

F

F

-

Q

-

K

T

T

I

I

V

I

I

F

V

V

T

S

H

H

N

D

M

L

Q

N

Q

E

A

A

A

L

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I

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D

L

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I

A

A

F

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M

Y

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L

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K

L

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I

M

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F

I

D

G

Q

T

T

G

E

E

R

E

I

I

F

L

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T

K

N

P

P

G

A

K

N

K

D

Q

Y

T

V

E

K

D

T

Y

F

I

V

binding phosphoaminophosphonic acid-adenylate ester: G62 (= G60), G64 (= G62), K65 (= K63), D187 (= D192), E188 (= E193)

Sites not aligning to the query:

7aheC Opua inhibited inward facing (see paper)
37% identity, 82% coverage: 44:266/271 of query aligns to 46:263/382 of 7aheC

query
sites
7aheC

S

N

M

F

D

E

I

I

P

N

E

E

K

G

Q

E

V

I

T

F

A

V

I

I

I

M

G

G

P

L

S

S

G

G

C

S

G

G

K

K

S

S

T

T

L

L

R

R

I

L

F

L

N

N

R

R

I

L

Y

-

A

-

I

I

Y

E

P

P

K

-

L

-

E

-

A

T

K

S

G

G

S

K

I

I

E

F

L

I

D

D

G

N

D

Q

N

D

I

V

-

A

-

T

L

L

D

N

P

K

G

E

Y

D

S

L

L

L

N

Q

R

V

L

R

R

R

S

K

K

T

V

M

G

S

M

M

V

V

F

F

Q

Q

K

N

P

F

V

G

P

L

F

F

P

P

M

H

-

R

T

T

I

I

F

L

E

E

N

N

V

T

A

E

Y

Y

G

G

I

L

R

-

H

E

H

V

E

Q

K

N

L

V

S

P

K

K

A

E

D

E

M

R

E

R

G

K

R

R

V

A

E

E

Q

K

A

A

L

L

R

D

S

N

A

A

A

N

L

L

W

L

D

D

E

-

V

F

K

K

D

D

K

Q

L

Y

R

P

Q

K

N

Q

A

-

L

-

G

-

L

L

S

S

G

G

Q

M

Q

Q

R

R

L

V

C

G

I

L

A

A

R

R

A

A

I

L

A

A

L

N

R

D

P

P

E

E

V

I

L

L

L

M

D

D

E

E

P

A

T

F

S

S

A

A

L

L

D

D

P

P

I

L

A

I

T

R

S

R

R

E

I

M

E

Q

Q

D

L

E

V

L

E

L

E

E

L

L

K

Q

S

A

Q

K

F

F

-

Q

-

K

T

T

I

I

V

I

I

F

V

V

T

S

H

H

N

D

M

L

Q

N

Q

E

A

A

A

L

R

R

V

I

S

G

D

D

L

R

T

I

A

A

F

I

M

M

Y

K

L

D

G

G

E

K

L

I

I

M

E

Q

F

I

D

G

Q

T

T

G

E

E

R

E

I

I

F

L

T

T

K

N

P

P

G

A

K

N

K

D

Q

Y

T

V

E

K

D

T

Y

F

I

V

Sites not aligning to the query:

binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 275, 297, 298

7y48B Cryo-em structure of biliverdin-bound mitochondrial abc transporter abcb10 from biortus (see paper)
33% identity, 85% coverage: 28:257/271 of query aligns to 341:565/567 of 7y48B

query
sites
7y48B

R

K

D

N

L

V

H

H

F

F

Y

A

Y

Y

D

P

G

A

-

R

-

P

-

E

F

V

Q

P

A

I

L

F

K

Q

G

D

I

F

S

S

M

L

D

S

I

I

P

P

E

S

K

G

Q

S

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V

T

T

A

A

I

L

I

V

G

G

P

P

S

S

G

G

C

S

G

G

K

K

S

S

T

T

L

V

L

L

R

S

I

L

F

L

N

L

R

R

I

L

Y

Y

A

D

I

-

Y

-

P

-

K

-

L

-

E

P

A

A

K

S

G

G

S

T

I

I

E

S

L

L

D

D

G

G

D

H

N

D

I

I

-

R

-

Q

L

L

D

N

P

P

G

V

Y

W

S

-

L

-

N

-

R

-

L

L

R

R

S

S

K

K

V

I

G

G

M

T

V

V

F

S

Q

Q

K

E

P

P

V

I

P

L

F

F

P

S

M

C

T

S

I

I

F

A

E

E

N

N

V

I

A

A

Y

Y

G

G

I

A

R

D

H

D

H

P

E

S

K

S

L

V

S

T

K

A

A

E

D

E

M

I

E

Q

G

-

R

R

V

V

E

A

Q

E

A

V

L

A

R

N

S

A

A

V

A

A

L

F

W

I

D

R

E

N

V

F

K

P

D

Q

K

G

L

F

R

-

Q

N

N

T