SitesBLAST
Comparing WP_007513608.1 NCBI__GCF_021560695.1:WP_007513608.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 7 hits to proteins with known functional sites (download)
Q8L7R2 Homoserine kinase; Protein DOWNY MILDEW RESISTANT 1; EC 2.7.1.39 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
39% identity, 97% coverage: 7:326/331 of query aligns to 48:368/370 of Q8L7R2
- G118 (= G83) mutation to R: In dmr1-6; loss of function.
- G180 (≠ A144) mutation to D: In dmr1-5; loss of function.
- G202 (= G167) mutation to R: In dmr1-4; loss of function.
- M241 (≠ A201) mutation to K: In dmr1-3; loss of function.
- A267 (≠ G227) mutation to V: In dmr1-1; loss of function.
Sites not aligning to the query:
- 46 E→K: In dmr1-2; loss of function.
P00547 Homoserine kinase; HK; HSK; EC 2.7.1.39 from Escherichia coli (strain K12) (see paper)
33% identity, 81% coverage: 25:291/331 of query aligns to 5:277/310 of P00547
- H139 (= H156) mutation to L: 35-fold decrease in kinase activity.
- H203 (≠ Q217) mutation to L: 2-fold decrease in kinase activity but nearly no change in substrates affinity.
- H206 (≠ N220) mutation to Q: 3500-fold decrease in kinase activity.
- R235 (= R249) mutation to H: 250-fold decrease in kinase activity but no change in substrates affinity.; mutation to L: 26200-fold decrease in catalytic efficiency.
1h74B Crystal structure of homoserine kinase complexed with ile (see paper)
31% identity, 78% coverage: 26:283/331 of query aligns to 7:264/296 of 1h74B
- active site: E126 (= E148), T179 (= T198)
- binding phosphothiophosphoric acid-adenylate ester: N58 (= N80), V59 (≠ T81), K83 (= K105), A87 (≠ L109), G88 (= G110), G90 (= G112), G92 (= G114), S93 (≠ G115), S94 (= S116), S97 (= S119), S129 (≠ A151)
- binding isoleucine: A12 (≠ G31), N13 (= N32), D19 (= D38), H134 (= H156), D136 (= D158), N137 (= N159), R183 (= R202), R231 (= R249), G256 (= G275)
1h74A Crystal structure of homoserine kinase complexed with ile (see paper)
31% identity, 78% coverage: 26:283/331 of query aligns to 7:264/296 of 1h74A
- active site: E126 (= E148), T179 (= T198)
- binding adenosine-5'-diphosphate: I51 (≠ L73), N58 (= N80), V59 (≠ T81), K83 (= K105), A87 (≠ L109), G88 (= G110), S93 (≠ G115), S94 (= S116), S97 (= S119), T179 (= T198)
- binding isoleucine: N13 (= N32), F18 (= F37), D19 (= D38), H134 (= H156), T179 (= T198), R183 (= R202), R231 (= R249)
1h73A Crystal structure of homoserine kinase complexed with threonine (see paper)
31% identity, 78% coverage: 26:283/331 of query aligns to 7:264/296 of 1h73A
- active site: E126 (= E148), T179 (= T198)
- binding phosphoaminophosphonic acid-adenylate ester: I51 (≠ L73), N58 (= N80), V59 (≠ T81), K83 (= K105), A87 (≠ L109), G88 (= G110), G90 (= G112), L91 (≠ M113), G92 (= G114), S93 (≠ G115), S94 (= S116), S97 (= S119)
- binding threonine: N13 (= N32), D19 (= D38), R183 (= R202), R231 (= R249)
1h72C Crystal structure of homoserine kinase complexed with hse (see paper)
31% identity, 78% coverage: 26:283/331 of query aligns to 7:264/296 of 1h72C
- active site: E126 (= E148), T179 (= T198)
- binding phosphoaminophosphonic acid-adenylate ester: I51 (≠ L73), N58 (= N80), V59 (≠ T81), K83 (= K105), A87 (≠ L109), G88 (= G110), G90 (= G112), L91 (≠ M113), G92 (= G114), S93 (≠ G115), S94 (= S116), S97 (= S119), S129 (≠ A151), T179 (= T198), S257 (≠ A276)
- binding l-homoserine: N13 (= N32), F18 (= F37), D19 (= D38), D136 (= D158), N137 (= N159), R183 (= R202), R231 (= R249)
1fwkA Crystal structure of homoserine kinase complexed with adp (see paper)
31% identity, 78% coverage: 26:283/331 of query aligns to 7:264/296 of 1fwkA
Query Sequence
>WP_007513608.1 NCBI__GCF_021560695.1:WP_007513608.1
MNSSLLEPVMAAPRTAKHPQVACAFAPASVGNVGVGFDLLGHSVAGAGDRAEVRRIDEPV
VRIAAIRGCVTDLPTDPRANTAGTALLSLRKALGLRHGFELTLHKGIALGSGMGGSAASC
VAALIAANALLPRPLPREALYGFALDGEAVASGSRHGDNLGSMLLGGLVLATHERLLRIE
VPEAWHCALVHPHVVLETRTARAALAGHYALGEFVAQSANLALVLAGCWRGDASLVREGL
KDVLVEPRRAPLIPNFARVKQAALDHRALGASISGAGPSVFGWYDHRADAEAASMAMRAA
FAEGGLDSDAWVSPIDGPAAALIASLDEALP
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory