SitesBLAST – Find functional sites

 

SitesBLAST

Comparing WP_007668613.1 NCBI__GCF_000319285.1:WP_007668613.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

P69797 PTS system mannose-specific EIIAB component; EIIAB-Man; EIII-Man; EC 2.7.1.191 from Escherichia coli (strain K12) (see 4 papers)
92% identity, 99% coverage: 1:318/320 of query aligns to 1:321/323 of P69797

query
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P69797
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1vrcB Complex of enzyme iiamannose and the histidine-containing phosphocarrier protein hpr from escherichia coli nmr, restrained regularized mean structure (see paper)
95% identity, 40% coverage: 2:130/320 of query aligns to 1:129/129 of 1vrcB

query
sites
1vrcB
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P26380 PTS system fructose-specific EIIB component; EIIB-Fru; Fructose-specific phosphotransferase enzyme IIB component; lev-PTS; p18; EC 2.7.1.202 from Bacillus subtilis (strain 168) (see paper)
46% identity, 50% coverage: 159:319/320 of query aligns to 2:162/163 of P26380

query
sites
P26380
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M
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P26379 PTS system fructose-specific EIIA component; EIIA-Fru; Fructose-specific phosphotransferase enzyme IIA component; lev-PTS; p16 from Bacillus subtilis (strain 168) (see paper)
36% identity, 33% coverage: 3:106/320 of query aligns to 2:105/146 of P26379

query
sites
P26379
I
 
I
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S
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V
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I
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A
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G
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E
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N
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E

Query Sequence

>WP_007668613.1 NCBI__GCF_000319285.1:WP_007668613.1
MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAETLIEKYNAQLEKLDTSK
GVVFLVDTWGGSPFNAASRIVVDKPNYEVVAGVNIPMLVETFMARDDDPSFDELVALAVE
TGREGVKALKAKPVEKAAPAAPAPKAAAAAKPMGPNDYMKIGLARIDDRLIHGQVATRWT
KETNVSRIIVVSDEVAADNVRKTLLTQVAPPGVTAHVVDVAKMIRVYNNPKYAGERVMLL
FTNPTDVERIVEGGVKITSVNIGGMAYRQGKTQVNNAVSVDEKDIEAFKKLNARGIELEV
RKVSNDPRLKMMDLISKVSH

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory