SitesBLAST – Find functional sites

 

SitesBLAST

Comparing WP_007679744.1 NCBI__GCF_000319285.1:WP_007679744.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 6 hits to proteins with known functional sites (download)

P69783 PTS system glucose-specific EIIA component; EIIA-Glc; EIII-Glc; Glucose-specific phosphotransferase enzyme IIA component from Escherichia coli (strain K12) (see 6 papers)
37% identity, 23% coverage: 473:617/619 of query aligns to 24:168/169 of P69783

query
sites
P69783
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K

Sites not aligning to the query:

1o2fA Complex of enzyme iiaglc and iibglc phosphocarrier protein hpr from escherichia coli nmr, restrained regularized mean structure (see paper)
37% identity, 23% coverage: 473:617/619 of query aligns to 5:149/150 of 1o2fA

query
sites
1o2fA
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P
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1glcF Cation promoted association (cpa) of a regulatory and target protein is controlled by phosphorylation (see paper)
37% identity, 23% coverage: 473:617/619 of query aligns to 16:160/161 of 1glcF

query
sites
1glcF
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P09323 PTS system N-acetylglucosamine-specific EIICBA component; EIICBA-Nag; EII-Nag; EC 2.7.1.193 from Escherichia coli (strain K12) (see paper)
36% identity, 25% coverage: 465:617/619 of query aligns to 490:647/648 of P09323

query
sites
P09323
Q
 
Q
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A
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P
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N
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A
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E
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A
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D
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C
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N
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Y
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K

Sites not aligning to the query:

P69786 PTS system glucose-specific EIICB component; EIICB-Glc; EII-Glc; EC 2.7.1.199 from Escherichia coli (strain K12) (see 5 papers)
42% identity, 10% coverage: 6:67/619 of query aligns to 401:461/477 of P69786

query
sites
P69786
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M
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A
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P
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A
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A
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A
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F
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G
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G
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K
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E
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N
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T
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N
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L
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A
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K
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K
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G
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Q
V
 
A
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x
I
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F
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G
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T

Sites not aligning to the query:

1o2fB Complex of enzyme iiaglc and iibglc phosphocarrier protein hpr from escherichia coli nmr, restrained regularized mean structure (see paper)
43% identity, 10% coverage: 6:66/619 of query aligns to 2:61/77 of 1o2fB

query
sites
1o2fB
L
 
M
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A
Q
 
A
D
 
A
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L
L
 
V
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A
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A
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F
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G
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G
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K
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E
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N
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I
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T
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N
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L
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D
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A
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R
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A
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D
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D
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A
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G
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L
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K
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K
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-
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A
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A
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G

Query Sequence

>WP_007679744.1 NCBI__GCF_000319285.1:WP_007679744.1
MDYQALAQDILARVGGKENISSVIHCATRLRFRLKDDRKADAPGLKAHPGVIMVVVSGGQ
FQVVIGNHVHDVWLAVRREAGITDDGAQAEDDGPRGNLLARLIDIVSGIFTPFIGVLAAS
GILKGLLALAVVCRWIDPASGTYKIWFAASDALFFFFPLVLGYTAGKKFGGNPFITMTIG
GALTHPVMISAFEASQQAGALADSFLGVPVIWFNYSSSVIPIILAAWVSCWLEKQGNRLL
PCAMKNFFTPLLCLGVTVPLTFLVIGPVATWLSQMLANGYQFIYTFAPWLAGAATGALWQ
VCVIFGLHWGLVPLMVNNIAVLGHDSLLPMLLPAVMGQVGAALAVFLRSRDARQKMLAGS
SVTAGIFGITEPAVYGVNLPLRRPFIFGCAAGAVGGAIVGLSSTQVYSFGFANIFTIAQM
IPPDGVNATLWGGLFGTLSALVLACGLTLIAGLPAGAKEANTGSQNVPQNAALAPLSGTV
LALDNVPDPTFASGLLGQGAAIIPSDNQVRAPFAGVVASLFETRHAIGLAGDNGMELLIH
VGIDTVKLEGQLFTAHVRVGDRVNPGDLLIEFDRQAIIDAGYDLTTPIIISNSDSYGAVT
TVAATAVSAGMPLLTVEPA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory