SitesBLAST

P26276 Phosphomannomutase/phosphoglucomutase; PMM / PGM; EC 5.4.2.2; EC 5.4.2.8 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 10 papers)
30% identity, 98% coverage: 3:448/454 of query aligns to 13:453/463 of P26276

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P26276

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R15 (≠ G5) mutation to A: KM halves, decreases processivity as dissociation of G1,6P intermediate increases 25-fold.
Y17 (≠ S7) binding alpha-D-glucose 1-phosphate; binding alpha-D-mannose 1-phosphate
R20 (= R10) mutation to A: No phosphoglucomutase activity.
S108 (= S94) binding via phosphate group; modified: Phosphoserine; mutation S->A,V: About 5% activity, still subject to substrate inhibition and requires G1,6P as an activator; phosphorylation occurs at a different site.; mutation to C: KM for G1P unchanged, kcat decreases 24-fold; G1,6P stimulates reaction by 2-3 orders of magnitude. No stable protein phosphorylation detected, altered ligation of metal residue.
N110 (= N96) mutation to A: KM halves, decreases processivity as dissociation of G1,6P intermediate increases 30-fold.
D242 (= D237) binding Mg(2+)
D244 (= D239) binding Mg(2+)
D246 (= D241) binding Mg(2+)
R247 (= R242) mutation to A: Small reduction in KM, small increase in dissociation of G1,6P intermediate.
R262 (≠ A257) mutation to A: Increases KM 2-fold, decreases kcat 9-fold for G1P. Alters flexibility of the hinge region.
K285 (≠ T278) binding alpha-D-glucose 1-phosphate
H308 (≠ S301) binding alpha-D-glucose 1-phosphate; binding alpha-D-mannose 1-phosphate
E325 (= E323) mutation to A: Reduces KM and Vmax approximately 2-fold.
EMSGH 325:329 (≠ EGNGG 323:327) binding alpha-D-glucose 1-phosphate; binding alpha-D-mannose 1-phosphate
H329 (≠ G327) mutation to A: No phosphoglucomutase activity using G1P as substrate, protein is less easily phosphorylated, no significant change in structure.
P368 (≠ I359) mutation to G: Increases KM 2-fold, decreases kcat 6-fold for G1P. Alters flexibility of the hinge region, structure is less compact.
R421 (= R416) mutation to C: Loss of phosphomannomutase activity, very low phosphoglucomutase activity.
RASNT 421:425 (≠ RESGT 416:420) binding alpha-D-glucose 1-phosphate; binding alpha-D-mannose 1-phosphate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

Q02E40 Phosphomannomutase/phosphoglucomutase; PMM / PGM; EC 5.4.2.2; EC 5.4.2.8 from Pseudomonas aeruginosa (strain UCBPP-PA14) (see paper)
30% identity, 98% coverage: 3:448/454 of query aligns to 13:453/463 of Q02E40

query
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Q02E40

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