SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_007691434.1 NCBI__GCF_000336675.1:WP_007691434.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 13 hits to proteins with known functional sites (download)

4xfjB Crystal structure of argininosuccinate synthase from mycobacterium thermoresistibile in complex with amppnp and arginine
40% identity, 92% coverage: 10:398/425 of query aligns to 1:393/397 of 4xfjB

query
sites
4xfjB

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active site: D13 (= D22), R94 (= R101), D123 (= D130), S174 (= S179)
binding phosphoaminophosphonic acid-adenylate ester: A7 (= A16), Y8 (≠ F17), S9 (= S18), T14 (= T23), I34 (≠ V44), G116 (= G123), C117 (= C124), F127 (= F134)
binding arginine: Y86 (= Y93), S90 (≠ T97), R126 (= R133), A183 (≠ S188), E185 (= E190), E259 (= E261), E269 (= E271), Y271 (= Y273)

7k5zA Crystal structure of argininosuccinate synthase from legionella pneumophila philadelphia 1 in complex with anppnp and a substrate analogue arginine
37% identity, 92% coverage: 12:401/425 of query aligns to 5:385/390 of 7k5zA

query
sites
7k5zA

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active site: D15 (= D22), R95 (= R101), D124 (= D130), S176 (= S179)
binding phosphoaminophosphonic acid-adenylate ester: A9 (= A16), Y10 (≠ F17), S11 (= S18), C37 (≠ V44), G117 (= G123), F128 (= F134)
binding arginine: Y88 (= Y93), T92 (= T97), D124 (= D130), R127 (= R133), S185 (= S188), E187 (= E190), E261 (= E261), Y273 (= Y273)

P59846 Argininosuccinate synthase; Citrulline--aspartate ligase; EC 6.3.4.5 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see 2 papers)
39% identity, 91% coverage: 12:399/425 of query aligns to 2:393/400 of P59846

query
sites
P59846

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6:14 (vs. 16:24, 78% identical) binding ATP
A33 (≠ V44) binding ATP
G114 (= G123) binding ATP

1j20A Crystal structure of thermus thermophilus hb8 argininosuccinate synthetase in complex with product (see paper)
38% identity, 91% coverage: 12:399/425 of query aligns to 2:384/386 of 1j20A

query
sites
1j20A

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K

E

E

E

F

V

A

E

E

E

A

A

E

R

E

E

T

K

A

A

E

L

A

R

L

T

D

G

L

A

E

S

H

K

Y

A

V

I

-

A

V

L

D

D

A

L

K

K

D

E

A

E

F

F

A

V

-

R

D

D

T

F

C

V

L

F

D

P

A

M

V

M

R

R

A

A

N

G

A

A

T

V

Y

Y

Q

E

G

G

Y

Y

P
x
Y

L

L

-

L

G

G

T
|
T

A

S

L

I

A

A

R
|
R

P

P

V

L

I

I

A

A

E

K

A

H

I

L

L

V

D

R

V

I

A

A

L

E

E

E

N

E

D

G

C

A

S

E

A

A

L

I

A

A

H
|
H

G
|
G

C
x
A

T
|
T

G

G

K

K

G
|
G

N
|
N

D
|
D

Q

Q

L

V

R
|
R

F
|
F

E

E

-

L

-

T

A

A

V

Y

W

A

R

L

D

K

S

P

E

D

L

I

D

K

V

V

I

I

A

A

P

P

V

W

R

R

E

E

L

W

G

S

L

F

T

Q

-

G

R

R

E

K

V

E

E

M

Q

I

E

A

Y

Y

A

A

D

E

E

A

R

H

D

G

L

I

P

P

V

V

E

P

G

-

N

-

A

-

G

-

A

P

W

Y

S
|
S

I

M

D

D

T

A

N

N

L

L

W

L

S

H

R

I

S
|
S

V

Y

E
|
E

G

G

S

G

E

V

L

L

E

E

E

D

P

P

N

W

Y

A

V

E

P

P

E

K

D

G

I

M

Y

F

D

R

W

M

T

T

Q

Q

S

D

P

P

-

E

-

A

S

P

G

D

E

A

T

P

E

E

L

Y

L

V

E

E

L

V

E

E

F

F

E

F

D

E

G

G

Y

D

P

P

V

V

A

A

L

V

D

N

G

G

E

E

A

R

M

L

D

S

P

P

I

A

E

A

L

L

I

L

E

Q

E

R

L

L

N

N

E

E

V

I

A

G

G

G

S

R

H

H

G

G

V

V

G

G

R

R

T

V

D

D

L

I

M

V

E
|
E

D

N

R

R

M

F

L

V

G

G

L

M

K

K

V

S

R

R

E

G

N

V

Y
|
Y

E

E

H

T

P

P

A

G

T

T

V

I

L

L

L

Y

N

H

A

A

H

R

E

R

A

A

L

V

E

E

S

S

L

L

V

T

L

L

T

D

K

R

E

E

E

V

R

L

S

H

F

Q

K

R

K

D

G

M

I

L

D

S

H

P

E

K

W

Y

A

A

E

E

K

L

A

V

Y

Y

E

Y

G

G

L

F

L

W

A

Y

A

A

P

P

L

E

V

R

D

E

A

A

L

L

D

Q

G

A

F

Y

I

F

D

D

S

H

T

V

Q

A

E

R

R

S

V

V

T

T

G

G

S

V

V

A

T

R

M

L

K

K

L

L

D

Y

G

K

G

G

Q

N

A

V

R

Y

A

V

V

V

G

G

R

R

E

K

S

A

E

P

Y

K

G

S

V

L

Y

Y

S

R

A

Q

A

D

A

L

A

V

S

S

F

F

N

-

T

-

A

-

D

-

V

-

G

-

G

G

I

Y

E

D

Q

Q

A

K

D

D

A

A

T

E

G

G

V

F

A

I

K

K

Y

I

H

Q

G

A

F

L

Q

R

S

L

R

R

L

V

A

R

S

A

R

L

V

V

active site: D12 (= D22), R92 (= R101), D121 (= D130), S168 (= S179)
binding adenosine monophosphate: A6 (= A16), T13 (= T23), A33 (≠ V44), R92 (= R101), H113 (= H122), G114 (= G123), F125 (= F134)
binding argininosuccinate: Y84 (≠ P94), T88 (= T97), A115 (≠ C124), T116 (= T125), G119 (= G128), N120 (= N129), D121 (= D130), R124 (= R133), S177 (= S188), E179 (= E190), E253 (= E261), Y265 (= Y273)

1j1zA Crystal structure of thermus thermophilus hb8 argininosuccinate synthetase in complex with substrate (see paper)
38% identity, 91% coverage: 12:399/425 of query aligns to 2:384/386 of 1j1zA

query
sites
1j1zA

R

K

V

I

A

V

L

L

A
|
A

F

Y

S

S

G

G

L

L

D
|
D

T
|
T

T

S

V

I

C

I

V

L

P

K

I

W

L

L

E

K

E

E

E

T

Y

Y

G

R

Y

-

D

A

E

E

V

V

V

I

G

A

V

F

T

T

V
x
A

D

D

V

I

G

G

Q

Q

P

-

T

G

K

E

E

E

F

V

A

E

E

E

A

A

E

R

E

E

T

K

A

A

E

L

A

R

L

T

D

G

L

A

E

S

H

K

Y

A

V

I

-

A

V

L

D

D

A

L

K

K

D

E

A

E

F

F

A

V

-

R

D

D

T

F

C

V

L

F

D

P

A

M

V

M

R

R

A

A

N

G

A

A

T

V

Y

Y

Q

E

G

G

Y

Y

P
x
Y

L

L

-

L

G

G

T
|
T

A

S

L

I

A

A

R
|
R

P

P

V

L

I
|
I

A

A

E

K

A

H

I

L

L

V

D

R

V

I

A

A

L

E

E

E

N

E

D

G

C

A

S

E

A

A

L

I

A

A

H
|
H

G
|
G

C
x
A

T
|
T

G

G

K

K

G
|
G

N
|
N

D
|
D

Q

Q

L

V

R
|
R

F
|
F

E

E

-

L

-

T

A

A

V

Y

W

A

R

L

D

K

S

P

E

D

L

I

D

K

V

V

I

I

A

A

P

P

V

W

R

R

E

E

L

W

G

S

L

F

T

Q

-

G

R

R

E

K

V

E

E

M

Q

I

E

A

Y

Y

A

A

D

E

E

A

R

H

D

G

L

I

P

P

V

V

E

P

G

-

N

-

A

-

G

-

A

P

W

Y

S
|
S

I
x
M

D

D

T

A

N

N

L

L

W

L

S

H

R

I

S
|
S

V

Y

E
|
E

G

G

S

G

E

V

L

L

E

E

E

D

P

P

N

W

Y

A

V

E

P

P

E

K

D

G

I

M

Y

F

D

R

W

M

T

T

Q

Q

S

D

P

P

-

E

-

A

S

P

G

D

E

A

T

P

E

E

L

Y

L

V

E

E

L

V

E

E

F

F

E

F

D

E

G

G

Y

D

P

P

V

V

A

A

L

V

D

N

G

G

E

E

A

R

M

L

D

S

P

P

I

A

E

A

L

L

I

L

E

Q

E

R

L

L

N

N

E

E

V

I

A

G

G

G

S

R

H

H

G

G

V

V

G

G

R

R

T

V

D

D

L

I

M

V

E
|
E

D

N

R

R

M

F

L

V

G

G

L

M

K

K

V

S

R

R

E

G

N

V

Y
|
Y

E

E

H

T

P

P

A

G

T

T

V

I

L

L

L

Y

N

H

A

A

H

R

E

R

A

A

L

V

E

E

S

S

L

L

V

T

L

L

T

D

K

R

E

E

E

V

R

L

S

H

F

Q

K

R

K

D

G

M

I

L

D

S

H

P

E

K

W

Y

A

A

E

E

K

L

A

V

Y

Y

E

Y

G

G

L

F

L

W

A

Y

A

A

P

P

L

E

V

R

D

E

A

A

L

L

D

Q

G

A

F

Y

I

F

D

D

S

H

T

V

Q

A

E

R

R

S

V

V

T

T

G

G

S

V

V

A

T

R

M

L

K

K

L

L

D

Y

G

K

G

G

Q

N

A

V

R

Y

A

V

V

V

G

G

R

R

E

K

S

A

E

P

Y

K

G

S

V

L

Y

Y

S

R

A

Q

A

D

A

L

A

V

S

S

F

F

N

-

T

-

A

-

D

-

V

-

G

-

G

G

I

Y

E

D

Q

Q

A

K

D

D

A

A

T

E

G

G

V

F

A

I

K

K

Y

I

H

Q

G

A

F

L

Q

R

S

L

R

R

L

V

A

R

S

A

R

L

V

V

active site: D12 (= D22), R92 (= R101), D121 (= D130), S168 (= S179)
binding aspartic acid: A115 (≠ C124), T116 (= T125), G119 (= G128), N120 (= N129), D121 (= D130)
binding adenosine-5'-triphosphate: A6 (= A16), T13 (= T23), A33 (≠ V44), R92 (= R101), I95 (= I104), H113 (= H122), G114 (= G123), F125 (= F134)
binding citrulline: Y84 (≠ P94), T88 (= T97), R124 (= R133), S168 (= S179), M169 (≠ I180), S177 (= S188), E179 (= E190), E253 (= E261), Y265 (= Y273)

1kh3A Crystal structure of thermus thermophilus hb8 argininosuccinate synthetase in complex with inhibitor (see paper)
38% identity, 91% coverage: 12:399/425 of query aligns to 2:378/380 of 1kh3A

query
sites
1kh3A

R

K

V

I

A

V

L

L

A
|
A

F

Y

S

S

G

G

L

L

D
|
D

T
|
T

T

S

V

I

C

I

V

L

P

K

I

W

L

L

E

K

E

E

E

T

Y

Y

G

R

Y

-

D

A

E

E

V

V

V

I

G

A

V

F

T
|
T

V
x
A

D

D

V

I

G

G

Q

Q

P

-

T

G

K

E

E

E

F

V

A

E

E

E

A

A

E

R

E

E

T

K

A

A

E

L

A

R

L

T

D

G

L

A

E

S

H

K

Y

A

V

I

-

A

V

L

D

D

A

L

K

K

D

E

A

E

F

F

A

V

-

R

D

D

T

F

C

V

L

F

D

P

A

M

V

M

R

R

A

A

N

G

A

A

T

V

Y

Y

Q

E

G

G

Y

Y

P
x
Y

L

L

-

L

G

G

T
|
T

A

S

L

I

A

A

R
|
R

P

P

V

L

I

I

A

A

E

K

A

H

I

L

L

V

D

R

V

I

A

A

L

E

E

E

N

E

D

G

C

A

S

E

A

A

L

I

A

A

H
|
H

G
|
G

C
x
A

T
|
T

G

G

K

K

G
|
G

N
|
N

D
|
D

Q

Q

L

V

R
|
R

F
|
F

E

E

-

L

-

T

A

A

V

Y

W

A

R

L

D

K

S

P

E

D

L

I

D

K

V

V

I

I

A

A

P

P

V

W

R

R

E

E

L

W

G

S

L

F

T

Q

-

G

R

R

E

K

V

E

E

M

Q

I

E

A

Y

Y

A

A

D

E

E

A

R

H

D

G

L

I

P

P

V

V

E

P

G

-

N

-

A

-

G

-

A

P

W

Y

S
|
S

I
x
M

D
|
D

T

A

N

N

L

L

W

L

S

H

R

I

S
|
S

V

Y

E
|
E

G

G

S

G

E

V

L

L

E

E

E

D

P

P

N

W

Y

A

V

E

P

P

E

K

D

G

I

M

Y

F

D

R

W

M

T

T

Q

Q

S

D

P

P

-

E

-

A

S

P

G

D

E

A

T

P

E

E

L

Y

L

V

E

E

L

V

E

E

F

F

E

F

D

E

G

G

Y

D

P

P

V

V

A

A

L

V

D

N

G

G

E

E

A

R

M

L

D

S

P

P

I

A

E

A

L

L

I

L

E

Q

E

R

L

L

N

N

E

E

V

I

A

G

G

G

S

R

H

H

G

G

V

V

G

G

R

R

T

V

D

D

L

I

M

V

E
|
E

D

N

R

R

M

F

L

V

G

G

L

M

K

K

V

S

R

R

E

G

N

V

Y
|
Y

E

E

H

T

P

P

A

G

T

T

V

I

L

L

L

Y

N

H

A

A

H

R

E

R

A

A

L

V

E

E

S

S

L

L

V

T

L

L

T

D

K

R

E

E

E

V

R

L

S

H

F

Q

K

R

K

D

G

M

I

L

D

S

H

P

E

K

W

Y

A

A

E

E

K

L

A

V

Y

Y

E

Y

G

G

L

F

L

W

A

Y

A

A

P

P

L

E

V

R

D

E

A

A

L

L

D

Q

G

A

F

Y

I

F

D

D

S

H

T

V

Q

A

E

R

R

S

V

V

T

T

G

G

S

V

V

A

T

R

M

L

K

K

L

L

D

Y

G

K

G

G

Q

N

A

V

R

Y

A

V

V

V

G

G

R

R

E

K

S

A

E

P

Y

K

G

S

V

L

Y

Y

S

R

A

-

S

-

F

-

N

-

T

-

A

-

D

-

V

-

G

-

G

G

I

Y

E

D

Q

Q

A

K

D

D

A

A

T

E

G

G

V

F

A

I

K

K

Y

I

H

Q

G

A

F

L

Q

R

S

L

R

R

L

V

A

R

S

A

R

L

V

V

active site: D12 (= D22), R92 (= R101), D121 (= D130), S168 (= S179)
binding phosphoaminophosphonic acid-adenylate ester: A6 (= A16), T13 (= T23), T32 (= T43), A33 (≠ V44), H113 (= H122), G114 (= G123), F125 (= F134), S168 (= S179), M169 (≠ I180)
binding arginine: Y84 (≠ P94), T88 (= T97), R124 (= R133), S168 (= S179), M169 (≠ I180), D170 (= D181), S177 (= S188), E179 (= E190), E253 (= E261), Y265 (= Y273)
binding aspartic acid: A115 (≠ C124), T116 (= T125), G119 (= G128), N120 (= N129), D121 (= D130)

P00966 Argininosuccinate synthase; Citrulline--aspartate ligase; EC 6.3.4.5 from Homo sapiens (Human) (see 16 papers)
36% identity, 92% coverage: 9:398/425 of query aligns to 3:412/412 of P00966

query
sites
P00966

S

S

T

K

D

G

R

S

V

V

A

V

L

L

A

A

F

Y

S

S

G

G

L

L

D

D

T

T

T

S

V

-

C

C

V

I

P

L

I

V

L

W

E

L

E

K

E

E

Y

Q

G

G

Y

Y

D

D

E

-

V

V

V

I

G

A

V

Y

T

L

V

A

D

N

V

I

G

G

Q

Q

P

-

T

K

K

E

E

D

F

F

A

E

E

E

A

A

E

R

E

K

T

K

A

A

E

L

A

K

L

L

D

G

L

A

E

K

H

K

-

V

Y

F

V

I

V

E

D

D

A
x
V

K

S

D

R

A

E

F

F

A

V

D

E

T

E

C

F

L

I

-

W

D

P

A

A

V

I

R

Q

A

S

N

S

A

A

T

L

Y

Y

Q

E

-

D

G

R

Y
|
Y

P

L

L

L

G

G

T
|
T

A
x
S

L

L

A

A

R
|
R

P
|
P

V

C

I

I

A

A

E

R

A

K

I

Q

L

V

D

E

V

I

A

A

L

Q

E

R

N

E

D

G

C

A

S

K

A

Y

L

V

A

S

H

H

G
|
G

C
x
A

T
|
T

G

G

K

K

G

G

N
|
N

D
|
D

Q

Q

L

V

R
|
R

F

F

E

E

A

L

V

S

W

C

R

Y

D

S

-

L

-

A

S

P

E

Q

L

I

D

K

V

V

I

I

A

A

P

P

V

W

R

R

E

M

L

P

G

E

L

F

T

Y

R

N

E

R

V

F

E

K

-

G

-
x
R

-

N

-

D

-

L

Q

M

E

E

Y

Y

A

A

D
x
K

E

Q

R

H

D

G

L

I

P

P

V

I

E

P

G

V

G

T

N

P

A
x
K

G

N

A

P

W
|
W

S
|
S

I

M

D

D

T

E

N

N

L

L

W

M

S

H

R

I

S
|
S

V

Y

E
|
E

G
x
A

S

G

E

I

L

L

E

E

E

N

P

P

N

K

Y

N

V

Q

P

A

P

P

E

P

D

G

I

L

Y

Y

D

T

W

K

T

T

Q

Q

S

D

P

P

S

A

-

K

-

A

-

P

G

N

E

T

T

P

E

D

L

I

L

L

E

E

L

I

E

E

F

F

E

K

D

K

G

G

Y

V

P

P

V

V

A

K

L

V

-

T

-

N

-

V

-

K

D

D

G

G

E

T

A

T

M

H

D

Q

-

T

P

S

I

L

E

E

L

L

I

F

E

M

E

Y

L

L

N

N

E

E

V

V

A

A

G

G

S

K

H

H

G

G

V
|
V

G

G

R
|
R

T

I

D

D

L

I

M

V

E
|
E

D

N

R
|
R

M

F

L

I

G

G

L

M

K

K

V

S

R

R

E
x
G

N

I

Y
|
Y

E

E

H
x
T

P

P

A

A

A

G

T

T

V

I

L

L

L

Y

N

H

A

A

H

H

E

L

A

D

L

I

E

E

S

A

L

F

V

T

L
x
M

T

D

K
x
R

E

E

E

V

R

R

S

K

F

I

K

K

K

Q

G

G

I

L

D

G

H

L

E

K

W

F

A

A

E

E

K

L

A

V

Y

Y

E

T

G
|
G

L

F

L

W

A

H

A

S

P

P

L

E

V

C

D

E

A

F

L

V

D

R

G

H

F

C

I

I

D

A

S

K

T

S

Q

Q

E

E

R

R

V

V

T

E

G
|
G

S

K

V

V

T

Q

M

V

K

S

L

V

D

L

G

K

G

G

Q

Q

A

V

R
x
Y

A

I

V

L

G
|
G

R

R

E

E

S

S

E

P

Y

L

G

S

V

L

Y

Y

S

N

A

E

A

L

A

V

S

S

F

M

N

N

T

-

A

-

D

-

V

V

G

Q

G

G

G

D

I

Y

E

E

Q

P

A

T

D

D

A

A

T

T

G
|
G

-

F

-

I

-

N

-

I

-

N

-

S

-

L

-

R

V

L

A

K

K

E

Y

Y

H

H

G

R

F

L

Q

Q

S

S

R

K

L

V

A

T

S

A

R

K

V64 (≠ A72) to I: in CTLN1; uncertain significance; dbSNP:rs556297791
Y87 (= Y93) binding L-citrulline
T91 (= T97) to P: in CTLN1; decreased affinity for aspartate; decreased affinity for citrulline; decreased argininosuccinate synthase activity; dbSNP:rs769018733
S92 (≠ A98) binding L-citrulline
R95 (= R101) to S: in CTLN1; increased thermal stability; loss of argininosuccinate synthase activity
P96 (= P102) to H: in CTLN1; decreased affinity for aspartate; decreased affinity for citrulline; decreased argininosuccinate synthase activity; to L: in CTLN1; decreased thermal stability; decreased affinity for aspartate; decreased affinity for citrulline; loss of argininosuccinate synthase activity; to S: in CTLN1; no effect on thermal stability; decreased argininosuccinate synthase activity
G117 (= G123) to S: in CTLN1; decreased thermal stability; loss of argininosuccinate synthase activity; dbSNP:rs770944877
A118 (≠ C124) to T: in CTLN1; decreased thermal stability; decreased affinity for aspartate; decreased affinity for citrulline; decreased argininosuccinate synthase activity; dbSNP:rs775305020
T119 (= T125) binding L-aspartate; to I: in CTLN1; decreased thermal stability; loss of argininosuccinate synthase activity
N123 (= N129) binding L-aspartate; binding L-citrulline
D124 (= D130) binding L-aspartate; to N: in CTLN1; loss of argininosuccinate synthase activity; dbSNP:rs936192871
R127 (= R133) binding L-citrulline; to L: increased thermal stability; loss of argininosuccinate synthase activity; dbSNP:rs201623252; to Q: in CTLN1; increased thermal stability; loss of argininosuccinate synthase activity; dbSNP:rs201623252; to W: in CTLN1; severe clinical course; loss of argininosuccinate synthase activity; dbSNP:rs771794639
R157 (vs. gap) to C: in CTLN1; decreased thermal stability; loss of argininosuccinate synthase activity; dbSNP:rs770585183; to H: in CTLN1; loss of argininosuccinate synthase activity; dbSNP:rs121908637
K165 (≠ D164) modified: N6-acetyllysine; by CLOCK; mutation K->Q,R: Significant loss of acetylation but no decrease in enzyme activity; when associated with Q-176 or R-176.
K176 (≠ A175) modified: N6-acetyllysine; by CLOCK; mutation K->Q,R: Significant loss of acetylation but no decrease in enzyme activity; when associated with Q-165 or R-165.
W179 (= W178) to R: in CTLN1; mild; decreased affinity for aspartate; decreased affinity for citrulline; decreased argininosuccinate synthase activity; dbSNP:rs121908646
S180 (= S179) binding L-citrulline; to I: in CTLN1; increased thermal stability; loss of argininosuccinate synthase activity; dbSNP:rs121908638; to N: in CTLN1; decreased thermal stability; decreased affinity for aspartate; decreased affinity for citrulline; decreased argininosuccinate synthase activity; dbSNP:rs121908638
S189 (= S188) binding L-citrulline
E191 (= E190) to Q: in CTLN1; loss of argininosuccinate synthase activity
A192 (≠ G191) to V: in CTLN1; decreased protein abundance
V263 (= V254) to M: in CTLN1; mild clinical course; no effect on affinity for aspartate; no effect on affinity for citrulline; decreased argininosuccinate synthase activity; dbSNP:rs192838388
R265 (= R256) to C: in CTLN1; severe clinical course; loss of argininosuccinate synthase activity; dbSNP:rs148918985
E270 (= E261) binding L-citrulline; to Q: in CTLN1; loss of argininosuccinate synthase activity; dbSNP:rs775163147
R272 (= R263) to C: in CTLN1; increased thermal stability; decreased affinity for aspartate; decreased affinity for citrulline; decreased argininosuccinate synthase activity; dbSNP:rs762387914; to H: in CTLN1; increased thermal stability; decreased affinity for aspartate; decreased affinity for citrulline; decreased argininosuccinate synthase activity; dbSNP:rs768215008; to L: in CTLN1; increased thermal stability; decreased affinity for aspartate; decreased affinity for citrulline; decreased argininosuccinate synthase activity; dbSNP:rs768215008
G280 (≠ E271) to R: in CTLN1; loss of argininosuccinate synthase activity
Y282 (= Y273) binding L-citrulline
T284 (≠ H275) to I: in CTLN1; mild clinical course; dbSNP:rs886039853
M302 (≠ L293) to V: in CTLN1; no effect on affinity for aspartate; no effect on affinity for citrulline; decreased argininosuccinate synthase activity
R304 (≠ K295) to W: in CTLN1; decreased protein abundance; dbSNP:rs121908642
G324 (= G315) to S: in CTLN1; loss of argininosuccinate synthase activity; dbSNP:rs121908639
G347 (= G338) to R: in CTLN1; severe clinical course
Y359 (≠ R350) to D: in CTLN1; mild clinical course
G362 (= G353) to V: in CTLN1; mild; no effect on affinity for aspartate; no effect on affinity for citrulline; decreased argininosuccinate synthase activity; dbSNP:rs121908647
G390 (= G384) to R: in CTLN1; loss of argininosuccinate synthase activity; dbSNP:rs121908641

2nz2A Crystal structure of human argininosuccinate synthase in complex with aspartate and citrulline (see paper)
36% identity, 90% coverage: 13:393/425 of query aligns to 4:402/402 of 2nz2A

query
sites
2nz2A

V

V

A

V

L

L

A

A

F

Y

S

S

G

G

L

L

D
|
D

T

T

T

S

V

-

C

C

V

I

P

L

I

V

L

W

E

L

E

K

E

E

Y

Q

G

G

Y

Y

D

D

E

-

V

V

V

I

G

A

V

Y

T

L

V

A

D

N

V

I

G

G

Q

Q

P

-

T

K

K

E

E

D

F

F

A

E

E

E

A

A

E

R

E

K

T

K

A

A

E

L

A

K

L

L

D

G

L

A

E

K

H

K

-

V

Y

F

V

I

V

E

D

D

A

V

K

S

D

R

A

E

F

F

A

V

D

E

T

E

C

F

L

I

-

W

D

P

A

A

V

I

R

Q

A

S

N

S

A

A

T

L

Y

Y

Q

E

-

D

G

R

Y
|
Y

P

L

L

L

G

G

T
|
T

A

S

L

L

A

A

R
|
R

P

P

V

C

I

I

A

A

E

R

A

K

I

Q

L

V

D

E

V

I

A

A

L

Q

E

R

N

E

D

G

C

A

S

K

A

Y

L

V

A

S

H

H

G

G

C
x
A

T
|
T

G

G

K

K

G
|
G

N
|
N

D
|
D

Q

Q

L

V

R
|
R

F

F

E

E

A

L

V

S

W

C

R

Y

D

S

-

L

-

A

S

P

E

Q

L

I

D

K

V

V

I

I

A

A

P

P

V

W

R

R

E

M

L

P

G

E

L

F

T

Y

R

N

E

R

V

F

E

K

Q

R

-

N

-

D

-

L

-

M

E

E

Y

Y

A

A

D

K

E

Q

R

H

D

G

L

I

P

P

V

I

E

P

G

V

G

T

N

P

A

K

G

N

A

P

W

W

S
|
S

I

M

D
|
D

T

E

N

N

L

L

W

M

S

H

R

I

S
|
S

V

Y

E
|
E

G

A

S

G

E

I

L

L

E

E

E

N

P

P

N

K

Y

N

V

Q

P

A

P

P

E

P

D

G

I

L

Y

Y

D

T

W

K

T

T

Q

Q

S

D

P

P

S

A

-

K

-

A

-

P

G

N

E

T

T

P

E

D

L

I

L

L

E

E

L

I

E

E

F

F

E

K

D

K

G

G

Y

V

P

P

V

V

A

K

L

V

-

T

-

N

-

V

-

K

D

D

G

G

E

T

A

T

M

H

D

Q

-

T

P

S

I

L

E

E

L

L

I

F

E

M

E

Y

L

L

N

N

E

E

V

V

A

A

G

G

S

K

H

H

G

G

V

V

G

G

R

R

T

I

D

D

L

I

M

V

E
|
E

D

N

R

R

M

F

L

I

G

G

L

M

K

K

V

S

R

R

E

G

N

I

Y
|
Y

E

E

H

T

P

P

A

A

A

G

T

T

V

I

L

L

L

Y

N

H

A

A

H

H

E

L

A

D

L

I

E

E

S

A

L

F

V

T

L

M

T

D

K

R

E

E

E

V

R

R

S

K

F

I

K

K

K

Q

G

G

I

L

D

G

H

L

E

K

W

F

A

A

E

E

K

L

A

V

Y

Y

E

T

G

G

L

F

L

W

A

H

A

S

P

P

L

E

V

C

D

E

A

F

L

V

D

R

G

H

F

C

I

I

D

A

S

K

T

S

Q

Q

E

E

R

R

V

V

T

E

G

G

S

K

V

V

T

Q

M

V

K

S

L

V

D

L

G

K

G

G

Q

Q

A

V

R

Y

A

I

V

L

G

G

R

R

E

E

S

S

E

P

Y

L

G

S

V

L

Y

Y

S

N

A

E

A

L

A

V

S

S

F

-

N

-

T

-

A

-

D

N

V

V

G

Q

G

G

G

D

I

Y

E

E

Q

P

A

T

D

D

A

A

T

T

G

G

-

F

-

I

-

N

-

I

-

N

-

S

-

L

-

R

V

L

A

K

K

E

Y

Y

H

H

G

R

F

L

Q

Q

S

S

active site: D13 (= D22), R92 (= R101), D121 (= D130), S176 (= S179)
binding aspartic acid: A115 (≠ C124), T116 (= T125), G119 (= G128), N120 (= N129), D121 (= D130)
binding citrulline: Y84 (= Y93), T88 (= T97), N120 (= N129), R124 (= R133), D178 (= D181), S185 (= S188), E187 (= E190), E266 (= E261), Y278 (= Y273)

1kp3A Crystal structure of e. Coli argininosuccinate synthetase in complex with atp and citrulline (see paper)
26% identity, 80% coverage: 12:352/425 of query aligns to 12:371/439 of 1kp3A

query
sites
1kp3A

R

R

V

I

A

G

L

I

A
|
A

F

F

S
|
S

G

G

G
|
G

L

L

D
|
D

T
|
T

T

S

V

A

C

A

V

L

P

L

I

W

L

M

E

R

E

Q

E

K

-

G

-

A

-

V

-

P

Y

Y

G

A

Y

Y

D

-

E

-

V

-

G

-

V

-

T
|
T

V
x
A

D

N

V

L

G

G

Q

Q

P

P

T

D

K

E

E

E

F

D

A

Y

E

D

A

A

-

I

-

P

-

R

-

A

-

M

E

E

E

Y

T

G

A

A

E

E

A

N

L

A

D

R

L

L

E

-

H

-

Y

-

V

-

V

I

D

D

A

C

K

R

D

K

A

Q

F

L

A

V

D

A

T

E

C

G

L

I

D

A

A

A

V

I

R

Q

A

C

N

G

A

A

T

F

Y

H

Q

N

G

T

-

T

-

G

-

L

-

T

Y
|
Y

P

F

L

N

G

T

T
|
T

A
x
P

L

L

A

G

R
|
R

P

A

V

V

I

T

A

G

E

T

A

M

I

L

L

V

D

A

V

A

A

M

L

K

E

E

N

D

D

G

C

V

S

N

A

I

L

W

A

G

H
x
D

G
|
G

C
x
S

T
|
T

G

Y

K

K

G
|
G

N
|
N

D
|
D

Q

I

L

E

R
|
R

F
|
F

E

Y

-

R

-

Y

A

G

V

L

W

L

R

T

D

N

S

A

E

E

L

L

D

Q

V

I

I

Y

A

K

P

P

-

W

-

L

-

D

-

T

-

D

-

F

V

I

R

D

E

E

L

L

G

G

L

G

T

R

R

H

E

E

V

M

E

S

Q

-

E

E

Y

F

A

M

D

I

E

A

R

C

D

G

L

F

P

D

V

Y

E

K

G

M

G

S

N

V

A

E

G

K

A

A

W

Y

S
|
S

I

T

D
|
D

T

S

N

N

L

M

W

L

S

G

R

A

S
x
T

V

H

E
|
E

G

A

S

K

E

D

L

L

E

E

E

Y

P

L

N

N

Y

S

V

S

-

V

-

K

-

I

-

V

-

N

P

P

P

I

E

M

D

G

I

V

Y

K

D

F

W

W

T

D

Q

E

S

S

P

V

S

K

G

I

E

P

T

A

E

E

L

E

L

V

E

T

L

V

E

R

F

F

E

E

D

Q

G

G

Y

H

P

P

V

V

A

A

L

L

D

N

G

G

E

K

A

T

M

F

-

S

D

D

P

D

I

V

E

E

L

M

I

M

E

L

E

E

L

A

N

N

E

R

V

I

A

G

G

G

S

R

H

H

G

G

V

L

G

G

R

M

T

S

D

D

L

Q

M

I

E
|
E

D

N

R

R

M

I

L

I

G

E

L

A

K

K

V
x
S

R

R

E

G

N

I

Y
|
Y

E

E

H

A

P

P

A

G

A

M

T

A

V

L

L

L

L

H

N

I

A

A

H

Y

E

E

A

R

L

L

E

L

S

T

L

G

V

I

L

H

T

N

K

E

E

D

E

T

R

I

S

E

F

Q

K

Y

K

H

G

A

I

H

D

G

H

R

E

Q

W

L

A

G

E

R

K

L

A

L

Y

Y

E

Q

G

G

L

R

L

W

-

F

-

D

-

S

-

Q

A

A

A

L

P

M

L

L

V

R

D

D

A

S

L

L

D

Q

G

R

F

W

I

V

D

A

S

S

T

-

Q

-

E

-

R

Q

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I

T

T

G

G

S

E

V

V

T

T

M

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E

L

L

D

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G

R

G

G

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N

A

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A

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active site: D22 (= D22), R106 (= R101), D135 (= D130), S191 (= S179)
binding adenosine-5'-triphosphate: A16 (= A16), S18 (= S18), G20 (= G20), D22 (= D22), T23 (= T23), T41 (= T43), A42 (≠ V44), D127 (≠ H122), G128 (= G123), S129 (≠ C124), F139 (= F134), D193 (= D181)
binding citrulline: Y98 (= Y93), T102 (= T97), P103 (≠ A98), T130 (= T125), G133 (= G128), N134 (= N129), D135 (= D130), R138 (= R133), D193 (= D181), T200 (≠ S188), E202 (= E190), E202 (= E190), E279 (= E261), S287 (≠ V269), Y291 (= Y273)

P0A6E4 Argininosuccinate synthase; Citrulline--aspartate ligase; EC 6.3.4.5 from Escherichia coli (strain K12) (see 4 papers)
26% identity, 80% coverage: 12:352/425 of query aligns to 13:372/447 of P0A6E4

query
sites
P0A6E4

R

R

V

I

A

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L

I

A
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A

F
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F

S
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S

G
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G

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L

D
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D

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T

T
x
S

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A

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A

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L

I

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E

R

E

Q

E

K

-

G

-

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-

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-

P

Y

Y

G

A

Y

Y

D

-

E

-

V

-

G

-

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-

T

T

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x
A

D

N

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L

G

G

Q

Q

P

P

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D

K

E

E

E

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D

A

Y

E

D

A

A

-

I

-

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-

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-

A

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M

E

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E

Y

T

G

A

A

E

E

A

N

L

A

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R

L

L

E

-

H

-

Y

-

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-

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I

D

D

A

C

K

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D

K

A

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F

L

A

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D

A

T

E

C

G

L

I

D

A

A

A

V

I

R

Q

A

C

N

G

A

A

T

F

Y

H

Q

N

G

T

-

T

-

G

-

L

-

T

Y
|
Y

P

F

L

N

G

T

T

T

A

P

L

L

A

G

R

R

P

A

V

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I

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A

G

E

T

A

M

I

L

L

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D

A

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A

A

M

L

K

E

E

N

D

D

G

C

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S

N

A

I

L

W

A

G

H

D

G
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G

C

S

T
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T

G

Y

K

K

G

G

N
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N

D
|
D

Q

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R
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R

F

F

E

Y

-

R

-

Y

A

G

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S

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D

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Y

A

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P

-

W

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L

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-

D

-

F

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D

E

E

L

L

G

G

L

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E

E

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M

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Q

-

E

E

Y

F

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I

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A

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C

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L

F

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D

V

Y

E

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N

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A

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G

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A

A

W

Y

S
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S

I

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D
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D

T

S

N

N

L

M

W

L

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G

R

A

S
x
T

V

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E
|
E

G

A

S

K

E

D

L

L

E

E

E

Y

P

L

N

N

Y

S

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S

-

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-

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-

I

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-

N

P

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A

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T

L

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F

F

E

E

D

Q

G

G

Y

H

P

P

V

V

A

A

L

L

D

N

G

G

E

K

A

T

M

F

-

S

D

D

P

D

I

V

E

E

L

M

I

M

E

L

E

E

L

A

N

N

E

R

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I

A

G

G

G

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R

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H

G

G

V

L

G

G

R

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D

L

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M

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E
|
E

D

N

R

R

M

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I

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L

A

K

K

V

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Y

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A

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P

A

G

A

M

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A

V

L

L

L

L

H

N

I

A

A

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Y

E

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A

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A

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Y

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G

G

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-

F

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D

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-

Q

A

A

A

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L

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D

A

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D

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A

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-

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R

Q

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G

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V

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T

M

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A

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I

17:25 (vs. 16:24, 89% identical) binding ATP
A43 (≠ V44) binding ATP
Y99 (= Y93) binding L-citrulline
G129 (= G123) binding ATP
T131 (= T125) binding ATP; binding L-aspartate
N135 (= N129) binding L-aspartate; binding L-citrulline
D136 (= D130) binding ATP; binding L-aspartate
R139 (= R133) binding L-citrulline
S192 (= S179) binding L-citrulline
D194 (= D181) binding ATP
T201 (≠ S188) binding L-citrulline
E203 (= E190) binding L-citrulline
E280 (= E261) binding L-citrulline

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1k97A Crystal structure of e. Coli argininosuccinate synthetase in complex with aspartate and citrulline (see paper)
26% identity, 80% coverage: 12:352/425 of query aligns to 12:371/432 of 1k97A

query
sites
1k97A

R

R

V

I

A

G

L

I

A

A

F

F

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S

G

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L

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D
|
D

T

T

T

S

V

A

C

A

V

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I

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L

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E

R

E

Q

E

K

-

G

-

A

-

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-

P

Y

Y

G

A

Y

Y

D

-

E

-

V

-

G

-

V

-

T

T

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A

D

N

V

L

G

G

Q

Q

P

P

T

D

K

E

E

E

F

D

A

Y

E

D

A

A

-

I

-

P

-

R

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A

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M

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E

E

Y

T

G

A

A

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E

A

N

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A

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L

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H

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D

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D

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A

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A

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L

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A

A

A

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N

G

A

A

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F

Y

H

Q

N

G

T

-

T

-

G

-

L

-

T

Y
|
Y

P

F

L

N

G

T

T
|
T

A
x
P

L

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A

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R
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R

P

A

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A

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A

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A

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A

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D

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A

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A

G

H

D

G

G

C
x
S

T
|
T

G

Y

K

K

G
|
G

N
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N

D
|
D

Q

I

L

E

R
|
R

F

F

E

Y

-

R

-

Y

A

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L

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A

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x
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D

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N

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L

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x
T

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E
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E

G

A

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K

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L

L

E

E

E

Y

P

L

N

N

Y

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S

-

V

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K

-

I

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N

P

P

P

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F

E

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D

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G

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P

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A

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N

G

G

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K

A

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F

-

S

D

D

P

D

I

V

E

E

L

M

I

M

E

L

E

E

L

A

N

N

E

R

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I

A

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G

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H

G

G

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L

G

G

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D

L

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M

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E
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E

D

N

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I

G

E

L

A

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K

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R

E

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N

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Y

E

E

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A

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P

A

G

A

M

T

A

V

L

L

L

L

H

N

I

A

A

H

Y

E

E

A

R

L

L

E

L

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T

L

G

V

I

L

H

T

N

K

E

E

D

E

T

R

I

S

E

F

Q

K

Y

K

H

G

A

I

H

D

G

H

R

E

Q

W

L

A

G

E

R

K

L

A

L

Y
|
Y

E

Q

G

G

L

R

L

W

-

F

-

D

-

S

-

Q

A

A

A

L

P

M

L

L

V

R

D

D

A

S

L

L

D

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R

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W

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A

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active site: D22 (= D22), R106 (= R101), D135 (= D130)