SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_007692480.1 NCBI__GCF_000336675.1:WP_007692480.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P07821 Iron(3+)-hydroxamate import ATP-binding protein FhuC; Ferric hydroxamate uptake protein C; Ferrichrome transport ATP-binding protein FhuC; Iron(III)-hydroxamate import ATP-binding protein FhuC; EC 7.2.2.16 from Escherichia coli (strain K12) (see 2 papers)
40% identity, 74% coverage: 55:294/326 of query aligns to 9:247/265 of P07821

query
sites
P07821

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K50 (= K97) mutation to Q: Lack of activity.
D172 (= D219) mutation to E: Lack of activity.
E173 (= E220) mutation to A: Lack of activity.

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
35% identity, 65% coverage: 73:283/326 of query aligns to 16:224/240 of 4ymuJ

query
sites
4ymuJ

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binding adenosine-5'-triphosphate: V16 (= V73), S36 (≠ N93), G37 (= G94), S38 (= S95), G39 (= G96), K40 (= K97), S41 (= S98), T42 (= T99), E162 (= E220), H194 (= H253)
binding magnesium ion: S41 (= S98), E162 (= E220)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 11

5x40A Structure of a cbio dimer bound with amppcp (see paper)
35% identity, 71% coverage: 56:285/326 of query aligns to 3:230/280 of 5x40A

query
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5x40A

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binding phosphomethylphosphonic acid adenylate ester: F14 (≠ Y67), V18 (≠ E71), A20 (≠ V73), N40 (= N93), G41 (= G94), G43 (= G96), K44 (= K97), S45 (= S98), T46 (= T99), Q88 (= Q139), H139 (≠ G193), M140 (≠ G194), L141 (= L195), S142 (= S196), G144 (= G198), Q145 (= Q199), Q166 (≠ E220), H198 (= H253)
binding magnesium ion: S45 (= S98), Q88 (= Q139)

5d3mA Folate ecf transporter: amppnp bound state (see paper)
33% identity, 70% coverage: 55:283/326 of query aligns to 3:231/280 of 5d3mA

query
sites
5d3mA

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binding phosphoaminophosphonic acid-adenylate ester: F13 (≠ L65), N42 (= N93), G43 (= G94), K46 (= K97), S47 (= S98)

6z5uK Cryo-em structure of the a. Baumannii mlabdef complex bound to appnhp (see paper)
32% identity, 67% coverage: 65:282/326 of query aligns to 8:226/253 of 6z5uK

query
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6z5uK

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binding phosphoaminophosphonic acid-adenylate ester: R12 (≠ E69), S37 (≠ N93), G38 (= G94), G40 (= G96), K41 (= K97), T42 (≠ S98), T43 (= T99), Q86 (= Q139), E164 (= E220), H197 (= H253)
binding magnesium ion: Q86 (= Q139), E164 (= E220)

7d0aB Acinetobacter mlafedb complex in adp-vanadate trapped vclose conformation (see paper)
32% identity, 67% coverage: 65:282/326 of query aligns to 10:228/263 of 7d0aB

query
sites
7d0aB

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G

V

E

V

V

R

L

L

L

D

D

G

G

R

K

A

D

I

I

Q

A

N

Q

L

M

D

S

T

R

K

Q

E

E

L

L

-

F

-

A

A

A

R

R

-

A

K

R

L

M

G

G

L

M

L

L

S

F

Q

Q

E

S

N

G

V

A

S

L

P

F

S

T

S

D

I

M

T

S

V

V

E

Y

D

E

L

N

V

V

L

A

H

F

G

P

R

I

Y

R

P

A

H

H

R

T

G

K

F

L

F

S

D

E

S

N

L

L

T

I

D

A

E

E

D

L

E

V

C

A

A

L

V

K

A

L

D

E

A

S

L

V

S

G

L

L

A

R

G

G

V

T

D

E

H

Q

L

L

R

M

D

P

R

T

E
|
E

V

-

G

-

L

L

S
|
S

G

G

Q
x
M

K

N

Q

R

L

R

A

V

W

A

I

L

A

A

M

R

V

A

L

I

A

A

Q

L

D

D

T

P

D

D

V

L

L

I

L

M

L

Y

D

D

E

E

P

P

T

F

T

A

F

G

L

Q

D

D

L

P

H

I

H

V

Q

K

L

G

E

V

V

L

M

T

E

R

I

L

I

I

E

R

T

S

L

L

K

R

A

E

D

A

S

L

E

D

V

L

T

T

I

T

V

I

L

I

V

V

L

S

H

H

D

D

I

V

D

P

Q

E

A

T

A

L

R

S

Y

I

A

A

D

D

H

Y

V

I

L

Y

A

V

L

V

D

A

D

E

G

G

S

K

I

I

Y

Q

A

G

R

E

G

G

P

T

P

P

E

E

V

L

binding adenosine-5'-diphosphate: S39 (≠ N93), T41 (≠ S95), G42 (= G96), K43 (= K97), E140 (= E191), S142 (= S196), M145 (≠ Q199)

7d08B Acinetobacter mlafedb complex in atp-bound vtrans1 conformation (see paper)
32% identity, 67% coverage: 65:282/326 of query aligns to 10:228/263 of 7d08B

query
sites
7d08B

L

L

G

S

Y

F

P

N

E
x
R

G

G

E

E

P
x
R

V

V

I

I

-

Y

D

D

G

N

E

I

S

S

V

L

S

N

I

I

P

R

Q

R

G

G

C

Q

V

I

T

T

A

A

L

I

V

M

G

G

P

P

N

S

G
|
G

S
x
T

G

G

K
|
K

S
x
T

T
|
T

L

L

R

R

G

L

L

I

S

G

N

G

Q

Q

L

L

T

V

P

P

D

D

D

Q

G

G

V

E

V

V

R

L

L

L

D

D

G

G

R

K

A

D

I

I

Q

A

N

Q

L

M

D

S

T

R

K

Q

E

E

L

L

-

F

-

A

A

A

R

R

-

A

K

R

L

M

G

G

L

M

L

L

S

F

Q

Q

E

S

N

G

V

A

S

L

P

F

S

T

S

D

I

M

T

S

V

V

E

Y

D

E

L

N

V

V

L

A

H

F

G

P

R

I

Y

R

P

A

H

H

R

T

G

K

F

L

F

S

D

E

S

N

L

L

T

I

D

A

E

E

D

L

E

V

C

A

A

L

V

K

A

L

D

E

A

S

L

V

S

G

L

L

A

R

G

G

V

T

D

E

H

Q

L

L

R

M

D

P

R

T

E

E

V

-

G

-

L

L

S

S

G

G

Q

M

K

N

Q

R

L

R

A

V

W

A

I

L

A

A

M

R

V

A

L

I

A

A

Q

L

D

D

T

P

D

D

V

L

L

I

L

M

L

Y

D

D

E
|
E

P

P

T

F

T

A

F

G

L

Q

D

D

L

P

H

I

H

V

Q

K

L

G

E

V

V

L

M

T

E

R

I

L

I

I

E

R

T

S

L

L

K

R

A

E

D

A

S

L

E

D

V

L

T

T

I

T

V

I

L

I

V

V

L

S

H

H

D

D

I

V

D

P

Q

E

A

T

A

L

R

S

Y

I

A

A

D

D

H

Y

V

I

L

Y

A

V

L

V

D

A

D

E

G

G

S

K

I

I

Y

Q

A

G

R

E

G

G

P

T

P

P

E

E

V

L

binding adenosine-5'-triphosphate: R14 (≠ E69), R17 (≠ P72), G40 (= G94), T41 (≠ S95), K43 (= K97), T44 (≠ S98), T45 (= T99), E166 (= E220)

Q5M244 Energy-coupling factor transporter ATP-binding protein EcfA2; ECF transporter A component EcfA2; EC 3.6.3.- from Streptococcus thermophilus (strain ATCC BAA-250 / LMG 18311) (see paper)
31% identity, 82% coverage: 58:325/326 of query aligns to 3:267/280 of Q5M244

query
sites
Q5M244

L

I

E

S

C

L

E

E

E

N

V

V

V

S

L

Y

G

T

Y

Y

P

Q

E

S

G

G

E

T

P

P

V

F

-

E

-

R

-

A

I

L

D

F

G

D

E

M

S

T

V

V

S

T

I

I

P

K

Q

D

G

G

C

S

V

Y

T

T

A

A

L

F

V

I

G

G

P

H

N

T

G

G

S

S

G

G

K

K

S

S

T

T

L

I

L

M

R

Q

G

L

L

L

S

N

N

G

Q

L

L

Y

T

L

P

P

D

T

D

S

G

G

V

Q

V

V

R

K

L

V

D

D

G

D

R

T

A

I

I

I

-

N

-

S

-

Q

-

S

Q

K

N

N

L

K

D

E

T

I

K

K

E

P

L

I

A

R

R

K

K

K

L

V

G

G

L

L

L

V

S

F

Q

Q

-

F

-

P

E

E

N

S

V
x
Q

S

L

P

F

S

A

S

E

I

T

T

V

V

L

E

E

D

D

L

I

V

A

L

F

H

G

G

-

R

-

Y

-

P

P

H

Q

R

N

G

-

F

-

F

F

D

G

S

V

L

S

T

K

D

E

E

E

D

A

E

E

C

Q

A

R

V

A

A

L

D

E

A

S

L

L

S

R

L

L

A

V

G

G

V

L

-

S

D

D

H

E

L

L

R

R

D

D

R

Q

E

N

V

P

G

F

G

D

L

L

S

S

G

G

Q

Q

K

M

Q

R

L

R

A

V

W

A

I

I

A

A

M

G

V

I

L

L

A

A

Q

M

D

Q

T

P

D

D

V

I

L

L

L

V

L

L

D

D

E
|
E

P

P

T

T

T

A

F

G

L

L

D

D

L

P

H

Q

H

G

Q

R

L

K

E

E

V

L

M

M

E

S

I

L

I

F

E

K

T

Q

L

L

K

H

A

L

D

-

S

S

E

G

V

I

T

T

I

I

V

V

L

L

V

V

L

T

H

H

D

L

I

M

D

D

Q

D

A

V

A

A

R

D

Y

Y

A

A

D

T

H

A

V

V

L

N

A

V

L

M

D

E

D

K

G

G

S

R

I

L

Y

V

A

L

R

S

G

G

P

T

P

P

E

K

E

-

V

-

I

-

T

-

E

-

D

-

L

-

H

-

D

D

V

V

F

F

G

Q

V

K

A

V

A

A

T

F

V

L

E

K

R

E

T

K

D

Q

R

L

G

G

-

V

P

P

R

K

V

I

T

T

A

E

H

F

N

A

A

L

V

Q

H

L

D

Q

A

E

D

K

G

G

N

Y

D

S

L

F

N

E

T

S

P

L

P

P

Q97 (≠ V142) mutation to A: No effect on ATPase, 10-fold decrease in riboflavin uptake; when associated with A-90 in EcfA1.
E171 (= E220) mutation to Q: 10-fold decrease in ATPase and riboflavin uptake; when associated with Q-163 in EcfA1.

8bmpA Cryo-em structure of the folate-specific ecf transporter complex in msp2n2 lipid nanodiscs bound to atp and adp (see paper)
33% identity, 70% coverage: 55:283/326 of query aligns to 3:228/278 of 8bmpA

query
sites
8bmpA

D

D

P

N

T

I

L

I

E

S

C

F

E

D

E

H

V

V

V

T

L
x
F

G

D

Y

S

P

P

E

R

G

-

E

-

P

P

V

A

I

L

D

S

G

D

E

L

S

S

V

F

S

A

I

I

P

E

Q

R

G

G

C

S

V

W

T

T

A

A

L

L

V

I

G

G

P

H

N
|
N

G

G

S

S

G
|
G

K
|
K

S
|
S

T
|
T

L

V

L

S

R

K

G

L

L

I

S

N

N

G

Q

L

L

L

T

A

P

P

D

D

-

L

-

D

-

K

G

S

V

S

V

I

R

T

L

V

D

D

G

G

R

V

A

K

I

L

Q

G

N

A

L

D

D

T

T

V

K

W

E

E

L

V

A

R

R

E

K

K

L

V

G

G

L

I

L

V

S

F

Q

Q

-

N

-

P

E

D

N

N

V

Q

S

F

P

V

S

G

S

A

I

T

T

V

V

S

E

D

D

D

L

V

V

A

-

F

-

G

L

L

H

E

G

N

R

R

Y

A

P

V

H

P

R

R

G

P

F

-

D

-

S

-

L

-

T

-

D

-

E

E

D

M

E

L

C

K

A

I

V

V

A

A

D

Q

A

A

L

V

S

A

L

D

A

V

G

G

V

M

D

A

H

D

L

Y

R

A

D

D

R

S

E

E

V

P

G

S

G

N

L

L

S

S

G

G

Q

Q

K

K

Q

Q

L

R

A

V

W

A

I

I

A

A

M

G

V

I

L

L

A

A

Q

V

D

K

T

P

D

Q

V

V

L

I

L

L

D

D

E

E

P

S

T

T

T

S

F

M

L

L

D

D

L

P

H

E

H

G

Q

K

L

E

E

Q

V

I

M

L

E

D

I

L

I

V

E

R

T

K

L

I

K

K

A

E

D

D

S

N

E

N

V

L

T

T

I

V

V

I

L

S

V

I

L

T

H

H

D

D

I

L

D

E

Q

E

A

A

R

G

Y

-

A

A

D

D

H

Q

V

V

L

L

A

V

L

L

D

D

G

G

S

Q

I

L

Y

L

A

D

R

Q

G

G

P

K

P

P

E

E

V

I

I

F

binding adenosine-5'-triphosphate: F13 (≠ L65), N39 (= N93), G42 (= G96), K43 (= K97), S44 (= S98), T45 (= T99)

4yerA Crystal structure of an abc transporter atp-binding protein (tm_1403) from thermotoga maritima msb8 at 2.35 a resolution
31% identity, 64% coverage: 74:282/326 of query aligns to 20:223/285 of 4yerA

query
sites
4yerA

I

V

D

K

G

G

E

V

S

S

V

F

S

S

I

V

P

K

Q

K

G

G

C

E

V

I

T

F

A

A

L

F

V

L

G

G

P

P

N
|
N

G
|
G

S

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

L

I

R

H

G

M

L

L

S

T

N

T

Q

L

L

L

T

K

P

P

D

T

D

S

G

G

V

K

V

A

R

W

L

V

D

A

G

G

R

H

A

D

I

V

Q

L

N

K

L

-

D

E

T

P

K

R

E

E

L

V

A

R

R

R

K

K

L

I

G

G

L

I

L

V

S

F

Q

Q

E

D

N

Q

V

S

S

L

P

D

S

R

S

E

I

L

T

T

-

A

V

Y

E

E

D

N

L

M

V

Y

L

I

H

H

G

G

R

K

-

I

Y

Y

P

G

H

Y

R

G

G

G

F

-

D

-

S

-

L

-

T

-

D

E

E

K

D

L

E

K

C

K

A

R

V

I

A

L

D

E

A

L

L

L

S

E

L

F

A

V

G

E

V

L

D

L

H

E

L

F

R

K

D

D

R

K

E

P

V

V

G

K

G
x
T

L
x
F

S
|
S

G

G

Q

M

K

A

Q

R

L

R

A

L

W

E

I

I

A

A

M

R

V

S

L

L

A

I

Q

H

D

E

T

P

D

E

V

V

L

L

L

F

L

L

D

D

E

E

P

P

T

T

T

I

F

G

L

L

D

D

L

P

H

H

H

T

Q

R

L

A

E

H

V

M

M

W

E

E

I

Y

I

I

E

S

T

K

L

M

K

K

A

K

D

E

S

H

E

N

V

M

T

T

I

I

V

F

L

L

V

T

L

T

H

H

D

Y

I

M

D

D

Q

E

A

A

A

E

R

Q

Y

L

A

A

D

D

H

R

V

V

L

A

A

I

L

I

D

D

D

H

G

G

S

K

I

I

Y

I

A

A

R

L

G

G

P

T

P

P

E

T

E

E

V

L

binding adenosine-5'-diphosphate: N39 (= N93), G40 (= G94), G42 (= G96), K43 (= K97), T44 (≠ S98), T45 (= T99), T135 (≠ G194), F136 (≠ L195), S137 (= S196)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 14, 17

8bmsA Cryo-em structure of the mutant solitary ecf module 2eq in msp2n2 lipid nanodiscs in the atpase closed and atp-bound conformation (see paper)
33% identity, 65% coverage: 72:283/326 of query aligns to 18:228/278 of 8bmsA

query
sites
8bmsA

P

P

V

A

I

L

D

S

G

D

E

L

S

S

V

F

S

A

I

I

P

E

Q

R

G

G

C

S

V

W

T

T

A

A

L

L

V

I

G

G

P

H

N
|
N

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

V

L

S

R

K

G

L

L

I

S

N

N

G

Q

L

L

L

T

A

P

P

D

D

-

L

-

D

-

K

G

S

V

S

V

I

R

T

L

V

D

D

G

G

R

V

A

K

I

L

Q

G

N

A

L

D

D

T

T

V

K

W

E

E

L

V

A

R

R

E

K

K

L

V

G

G

L

I

L

V

S

F

Q
|
Q

-

N

-

P

E

D

N

N

V

Q

S

F

P

V

S

G

S

A

I

T

T

V

V

S

E

D

D

D

L

V

V

A

-

F

-

G

L

L

H

E

G

N

R

R

Y

A

P

V

H

P

R

R

G

P

F

-

D

-

S

-

L

-

T

-

D

-

E

E

D

M

E

L

C

K

A

I

V

V

A

A

D

Q

A

A

L

V

S

A

L

D

A

V

G

G

V

M

D

A

H

D

L
x
Y

R

A

D

D

R

S

E

E

V

P

G

S

G
x
N

L

L

S
|
S

G
|
G

Q
|
Q

K

K

Q

Q

L

R

A

V

W

A

I

I

A

A

M

G

V

I

L

L

A

A

Q

V

D

K

T

P

D

Q

V

V

L

I

L

L

D

D

E
x
Q

P

S

T

T

T

S

F

M

L

L

D

D

L

P

H

E

H

G

Q

K

L

E

E

Q

V

I

M

L

E

D

I

L

I

V

E

R

T

K

L

I

K

K

A

E

D

D

S

N

E

N

V

L

T

T

I

V

V

I

L

S

V

I

L

T

H
|
H

D

D

I

L

D

E

Q

E

A

A

R

G

Y

-

A

A

D

D

H

Q

V

V

L

L

A

V

L

L

D

D

G

G

S

Q

I

L

Y

L

A

D

R

Q

G

G

P

K

P

P

E

E

V

I

I

F

binding adenosine-5'-triphosphate: N39 (= N93), G40 (= G94), S41 (= S95), G42 (= G96), K43 (= K97), S44 (= S98), T45 (= T99), Q88 (= Q139), Y133 (≠ L187), N140 (≠ G194), S142 (= S196), G143 (= G197), G144 (= G198), Q145 (= Q199), Q166 (≠ E220), H199 (= H253)
binding magnesium ion: S44 (= S98), Q88 (= Q139)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12

Q9NRK6 ATP-binding cassette sub-family B member 10, mitochondrial; ABC-mitochondrial erythroid protein; ABC-me protein; ATP-binding cassette transporter 10; ABC transporter 10 protein; Mitochondrial ATP-binding cassette 2; M-ABC2 from Homo sapiens (Human) (see 5 papers)
34% identity, 70% coverage: 57:285/326 of query aligns to 491:721/738 of Q9NRK6

query
sites
Q9NRK6

T

A

L

L

E

E

C

F

E

K

E

N

V

V

V

H

L

F

G

A

Y

Y

P

P

E

A

G

R

E

P

-

E

-

V

P

P

V

I

I

F

D

Q

G

D

E

F

S

S

V

L

S

S

I

I

P

P

Q

S

G

G

C

S

V

V

T

T

A

A

L

L

V

V

G

G

P

P

N

S

G

G

S

S

G

G

K
|
K

S

S

T

T

L

V

L

L

R

S

G

L

L

L

S

L

N

R

Q

L

L

Y

T
x
D

P

P

D

A

D

S

G

G

V

T

V

I

R

S

L

L

D

D

G

G

R

H

A

D

I

I

Q

R

N

Q

L

L

D

N

T

P

K

V

E

W

L

L

A

R

R

S

K

K

L

I

G

G

L

T

L

V

S

S

Q

Q

E

E

N

P

V

I

S

L

P

F

S

S

S
x
C

I

S

T

I

V

A

E

E

D

N

L

I

V

A

L

Y

H

G

G

A

R

D

Y

D

P

P

H

-

R

-

G

-

F

-

D

S

S

S

L

V

T

T

D

A

E

E

D

E

E

I

C

Q

A

R

V

V

A

A

D

E

A

V

L

A

S

N

L

-

A

-

G

A

V

V

D

A

H

F

L

I

R

R

D

N

R

F

E

P

V

Q

G

G

G

F

-

N

-

T

-

V

-

G

-

E

-

K

-

G

-

V

-

L

L

L

S
|
S

G

G

Q
|
Q

K

K

Q

Q

L

R

A

I

W

A

I

I

A

A

M

R

V

A

L

L

A

L

Q

K

D

N

T

P

D

K

V

I

L

L

D
|
D

E
|
E

P

A

T

T

T

S

F

A

L

L

D

D

L

A

H

E

H

N

Q

E

L

Y

E

L

V

V

M

Q

E

E

I

A

I

L

E

D

T

R

L

L

K

M

A

D

D

G

S

R

E

-

V

-

T

T

I

V

V

L

L

V

V

I

L

A

H

H

D

R

I

L

D

S

Q

-

A

T

A

I

R

K

Y

N

A

A

D

N

H

M

V

V

L

A

A

V

L

L

D

D

D

Q

G

G

S

K

I

I

Y

T

A

E

R

Y

G

G

P

K

P

H

E

E

V

L

I

L

T

S

E

K

K533 (= K97) mutation to E: Increases hemoglobin biosynthetic process.
D545 (≠ T109) to N: in dbSNP:rs35698797
C582 (≠ S146) mutation to G: Does not affect ATPase activity; when associated with S-215 and L-224. Activated by Zn (II) mesoporphyrin; when associated with S-215 and L-224.
S635 (= S196) mutation to R: Does not rescue hemoglobin and heme biosynthetic process.
Q638 (= Q199) mutation to H: Does not rescue hemoglobin and heme biosynthetic process.
D658 (= D219) mutation to A: Does not rescue hemoglobin and heme biosynthetic process.
E659 (= E220) mutation to A: Does not rescue hemoglobin and heme biosynthetic process.

Sites not aligning to the query:

150 A → S: in dbSNP:rs4148756
215 C→S: Does not affect ATPase activity; when associated with L-224 and G-582. Activated by Zn (II) mesoporphyrin; when associated with L-224 and G-582.
224 C→L: Does not affect ATPase activity; when associated withS-215 and G-582. Activated by Zn (II) mesoporphyrin; when associated with S-215 and G-582.
471 R → T: in a breast cancer sample; somatic mutation

4ayxA Structure of the human mitochondrial abc transporter, abcb10 (rod form b) (see paper)
33% identity, 74% coverage: 44:285/326 of query aligns to 316:568/571 of 4ayxA

query
sites
4ayxA

L

L

T

E

D

R

E

E

P

P

D

K

V

L

P

P

A

F

D

N

R

E

-

G

-

V

-

I

-

L

-

N

-

E

-

K

-

S

-

F

D

Q

P

G

T

A

L

L

E

E

C

F

E

K

E

N

V

V

V

H

L

F

G

A

Y
|
Y

P

P

E

A

G

R

E

P

-

E

-

V

P

P

V

I

I

F

D

Q

G

D

E

F

S

S

V

L

S

S

I

I

P

P

Q

S

G

G

C

S

V

V

T

T

A

A

L

L

V

V

G

G

P

P

N
x
S

G
|
G

S

S

G
|
G

K
|
K

S
|
S

T
|
T

L

V

L

L

R

S

G

L

L

L

S

L

N

R

Q

L

L

Y

T

D

P

P

D

A

D

S

G

G

V

T

V

I

R

S

L

L

D

D

G

G

R

H

A

D

I

I

Q

R

N

Q

L

L

D

N

T

P

K

V

E

W

L

L

A

R

R

S

K

K

L

I

G

G

L

T

L

V

S

S

Q
|
Q

E

E

N

P

V

I

S

L

P

F

S

S

S

C

I

S

T

I

V

A

E

E

D

N

L

I

V

A

L

Y

H

G

G

A

R

D

Y

D

P

P

H

-

R

-

G

-

F

-

D

S

S

S

L

V

T

T

D

A

E

E

D

E

E

I

C

Q

A

R

V

V

A

A

D

E

A

V

L

A

S

N

L

-

A

-

G

A

V

V

D

A

H

F

L

I

R

R

D

N

R

F

E

P

V

Q

G

G

G

F

-

N

-

T

-

V

-

G

-

E

-

K

-

G

-

V

-

L

L

L

S

S

G

G

Q

Q

K

K

Q

Q

L

R

A

I

W

A

I

I

A

A

M

R

V

A

L

L

A

L

Q

K

D

N

T

P

D

K

V

I

L

L

D

D

E

E

P

A

T

T

T

S

F

A

L

L

D

D

L

A

H

E

H

N

Q

E

L

Y

E

L

V

V

M

Q

E

E

I

A

I

L

E

D

T

R

L

L

K

M

A

D

D

G

S

R

E

-

V

-

T

T

I

V

V

L

L

V

V

I

L

A

H

H

D

R

I

L

D

S

Q

-

A

T

A

I

R

K

Y

N

A

A

D

N

H

M

V

V

L

A

A

V

L

L

D

D

D

Q

G

G

S

K

I

I

Y

T

A

E

R

Y

G

G

P

K

P

H

E

E

V

L

I

L

T

S

E

K

binding phosphomethylphosphonic acid adenylate ester: Y348 (= Y67), S376 (≠ N93), G377 (= G94), G379 (= G96), K380 (= K97), S381 (= S98), T382 (= T99)
binding magnesium ion: S381 (= S98), Q422 (= Q139)

Sites not aligning to the query:

binding glycine: 17, 81

7y48B Cryo-em structure of biliverdin-bound mitochondrial abc transporter abcb10 from biortus (see paper)
33% identity, 74% coverage: 44:285/326 of query aligns to 315:564/567 of 7y48B

query
sites
7y48B

L

L

T

E

D

R

E

E

P

P

D

K

V

L

P

P

A

F

D

N

R

E

-

G

-

V

-

I

-

L

-

N

-

E

-

K

-

S

-

F

D

Q

P

G

T

A

L

L

E

E

C

F

E

K

E

N

V

V

V

H

L

F

G

A

Y

Y

P

P

E

A

G

R

E

P

-

E

-

V

P

P

V

I

I

F

D

Q

G

D

E

F

S

S

V

L

S

S

I

I

P

P

Q

S

G

G

C

S

V

V

T

T

A

A

L

L

V

V

G

G

P

P

N

S

G

G

S

S

G

G

K

K

S

S

T

T

L

V

L

L

R

S

G

L

L

L

S

L

N

R

Q

L

L

Y

T

D

P

P

D

A

D

S

G

G

V

T

V

I

R

S

L

L

D

D

G

G

R

H

A

D

I

I

Q

R

N

Q

L

L

D

N

T

P

K

V

E

W

L

L

A

R

R

S

K

K

L

I

G

G

L

T

L

V

S

S

Q

Q

E

E

N

P

V

I

S

L

P

F

S

S

S

C

I

S

T

I

V

A

E

E

D

N

L

I

V

A

L

Y

H

G

G

A

R

D

Y

D

P

P

H

-

R

-

G

-

F

-

D

S

S

S

L

V

T

T

D

A

E

E

D

E

E

I

C

Q

A

R

V

V

A

A

D

E

A

V

L

A

S

N

L

-

A

-

G

A

V

V

D

A

H

F

L

I

R

R

D

N

R

F

E

P

V

Q

G

G

G

F

-

N

-

T

-

V

-

G

-

V

-

L

L

L

S

S

G

G

Q

Q

K

K

Q

Q

L

R

A

I

W

A

I

I

A

A

M

R

V

A

L

L

A

L

Q

K

D

N

T

P

D

K

V

I

L

L

D

D

E

Q

P

A

T

T

T

S

F

A

L

L

D

D

L

A

H

E

H

N

Q

E

L

Y

E

L

V

V

M

Q

E

E

I

A

I

L

E

D

T

R

L

L

K

M

A

D

D

G

S

R

E

-

V

-

T

T

I

V

V

L

L

V

V

I

L

A

H

H

D

R

I

L

D

S

Q

-

A

T

A

I

R

K

Y

N

A

A

D

N

H

M

V

V

L

A

A

V

L

L

D

D

D

Q

G

G

S

K

I

I

Y

T

A

E

R

Y

G

G

P

K

P

H

E

E

V

L

I

L

T

S

E

K

Sites not aligning to the query:

binding Biliverdine IX Alpha: 171, 248, 252, 255

F1MWM0 Retinal-specific phospholipid-transporting ATPase ABCA4; ATP-binding cassette sub-family A member 4; RIM ABC transporter; RIM protein; RmP; Retinal-specific ATP-binding cassette transporter; EC 7.6.2.1 from Bos taurus (Bovine) (see 2 papers)
29% identity, 65% coverage: 68:279/326 of query aligns to 940:1144/2281 of F1MWM0

query
sites
F1MWM0

P

P

E

Y

G

G

E

R

P

P

V

A

I

V

D

D

G

R

E

L

S

N

V

I

S

T

I

F

P

Y

Q

E

G

S

C

Q

V

I

T

T

A

A

L

F

V

L

G

G

P

H

N

N

G

G

S

A

G

G

K

K

S

T

T

T

L

T

L

L

R

S

G

I

L

M

S

T

N

G

Q

L

L

L

T

P

P

P

D

T

D

S

G

G

V

T

V

V

R

L

L

V

D

G

G

G

R

K

A

D

I

I

Q

E

-

T

N

N

L

L

D

D

T

A

K

-

E

-

L

I

A

R

R

Q

K

S

L

L

G

G

L

M

L

C

S

P

Q

Q

E

H

N

N

V

I

S

L

P

F

S

H

I

L

T

T

V

V

-

A

E

E

D

H

L

I

V

L

L

F

H

Y

G

A

R

Q

Y

L

P

K

H

G

R

R

G

S

F

W

F

-

D

-

S

-

L

-

T

-

D

D

E

K

D

A

E

Q

C

L

A

E

V

M

A

E

D

A

A

M

L

L

S

E

L

D

A

T

G

G

V

L

D

H

H

H

L

K

R

R

D

N

R

E

E

E

V

A

G

Q

G

D

L

L

S

S

G

G

Q

V

K

Q

Q

R

L

K

A

L

W

S

I

V

A

A

M

I

V

A

L

F

A

V

Q

G

D

D

T

A

D

K

V

V

L

V

L

L

D

D

E

E

P

P

T

T

T

S

F

G

L

V

D

D

L

P

H

Y

H

S

Q

R

L

R

E

S

V

I

M

W

E

D

I

L

E

-

T

-

L

L

K

K

A

Y

D

R

S

S

E

G

V

R

T

T

I

I

V

I

L

M

V

S

L

T

H

H

D

H

I

M

D

D

Q

E

A

A

A

D

R

I

Y

L

A

G

D

D

H

R

V

I

L

A

A

I

L

I

D

S

D

Q

G

G

S

R

I

L

Y

Y

A

C

R

S

G

G

P

T

P

P

Sites not aligning to the query:

415 modified: carbohydrate, N-linked (Hex...) asparagine
504 modified: carbohydrate, N-linked (Hex...) asparagine
901 modified: Phosphothreonine; T→A: Decreases expression level. Affects subcellular location.
1185 modified: Phosphoserine; S→A: Does not affect subcellular location. Does not affect expression level. Does not affect ATPase activity. Reduces the stimulating effect of all-trans-retinal on ATP hydrolysis.
1309 Cleavage; by trypsin
1313 modified: Phosphothreonine; T→A: Does not affect subcellular location. Does not affect expression level. Does not affect ATPase activity. Reduces the stimulating effect of all-trans-retinal on ATP hydrolysis.
1317 modified: Phosphoserine; S→A: Does not affect subcellular location. Does not affect expression level. Affects both the basal and stimulated ATPase activity.
1319 modified: Phosphoserine
1455 modified: carbohydrate, N-linked (Hex...) asparagine
1527 modified: carbohydrate, N-linked (Hex...) asparagine
1660 modified: carbohydrate, N-linked (Hex...) asparagine

8f5bA Human abca4 structure in complex with amp-pnp
29% identity, 65% coverage: 68:279/326 of query aligns to 732:936/1924 of 8f5bA

query
sites
8f5bA

P

P

E

C

G

G

E

R

P

P

V

A

I

V

D

D

G

R

E

L

S

N

V

I

S

T

I

F

P

Y

Q

E

G

N

C

Q

V

I

T

T

A

A

L

F

V

L

G

G

P

H

N
|
N

G
|
G

S

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

L

L

R

S

G

I

L

L

S

T

N

G

Q

L

L

L

T

P

P

P

D

T

D

S

G

G

V

T

V

V

R

L

L

V

D

G

G

G

R

R

A

D

I

I

Q

E

-

T

N

S

L

L

D

D

T

A

K

-

E

-

L

V

A

R

R

Q

K

S

L

L

G

G

L

M

L

C

S

P

Q
|
Q

E

H

N

N

V

I

S

L

P

F

S

H

I

L

T

T

V

V

E

A

D

E

L

-

V

-

L

-

H

-

G

-

R

-

Y

-

P

-

H

H

R

M

G

L

F

F

F

Y

D

A

S

Q

L

L

T

K

-

G

-

K

-

S

-

Q

D

E

E

E

D

A

E

Q

C

L

A

E

V

M

A

E

D

A

A

M

L

L

S

E

L

D

A

T

G

G

V

L

D

H

H

H

L

K

R

R

D

N

R

E

E

E

V

A

G

Q

G
x
D

L

L

S

S

G

G

Q

M

K

Q

Q

R

L

K

A

L

W

S

I

V

A

A

M

I

V

A

L

F

A

V

Q

G

D

D

T

A

D

K

V

V

L

V

L

I

L

L

D

D

E

E

P

P

T

T

T

S

F

G

L

V

D

D

L

P

H

Y

H

S

Q

R

L

R

E

S

V

I

M

W

E

D

I

L

E

-

T

-

L

L

K

K

A

Y

D

R

S

S

E

G

V

R

T

T

I

I

V

I

L

M

V

S

L

T

H

H

D

H

I

M

D

D

Q

E

A

A

A

D

R

L

Y

L

A

G

D

D

H

R

V

I

L

A

A

I

L

I

D

A

D

Q

G

G

S

R

I

L

Y

Y

A

C

R

S

G

G

P

T

P

P

binding phosphoaminophosphonic acid-adenylate ester: N757 (= N93), G758 (= G94), G760 (= G96), K761 (= K97), T762 (≠ S98), T763 (= T99), Q802 (= Q139), D853 (≠ G194)
binding magnesium ion: T762 (≠ S98), Q802 (= Q139)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 1652, 1653, 1654, 1655, 1656, 1657, 1697, 1748, 1750
binding magnesium ion: 1657, 1697

P78363 Retinal-specific phospholipid-transporting ATPase ABCA4; ATP-binding cassette sub-family A member 4; RIM ABC transporter; RIM proteinv; RmP; Retinal-specific ATP-binding cassette transporter; Stargardt disease protein; EC 7.6.2.1 from Homo sapiens (Human) (see 43 papers)
29% identity, 65% coverage: 68:279/326 of query aligns to 940:1144/2273 of P78363

query
sites
P78363

P
|
P

E
x
C

G
|
G

E
x
R

P
|
P

V
x
A

I
x
V

D
|
D

G
x
R

E
x
L

S
x
N

V
x
I

S
x
T

I
x
F

P
x
Y

Q
x
E

G
x
N

C
x
Q

V
x
I

T
|
T

A
|
A

L
x
F

V
x
L

G
|
G

P
x
H

N
|
N

G
|
G

S
x
A

G
|
G

K
|
K