SitesBLAST
Comparing WP_007693272.1 NCBI__GCF_000336675.1:WP_007693272.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
1ji0A Crystal structure analysis of the abc transporter from thermotoga maritima
46% identity, 86% coverage: 38:268/269 of query aligns to 6:238/240 of 1ji0A
6mjpA Lptb(e163q)fgc from vibrio cholerae (see paper)
33% identity, 86% coverage: 38:268/269 of query aligns to 2:235/240 of 6mjpA
1g6hA Crystal structure of the adp conformation of mj1267, an atp-binding cassette of an abc transporter (see paper)
29% identity, 83% coverage: 37:259/269 of query aligns to 3:240/254 of 1g6hA
1g9xB Characterization of the twinning structure of mj1267, an atp-binding cassette of an abc transporter (see paper)
29% identity, 83% coverage: 37:259/269 of query aligns to 3:240/253 of 1g9xB
6s8nB Cryo-em structure of lptb2fgc in complex with lipopolysaccharide (see paper)
34% identity, 86% coverage: 39:268/269 of query aligns to 3:235/238 of 6s8nB
6s8gA Cryo-em structure of lptb2fgc in complex with amp-pnp (see paper)
34% identity, 86% coverage: 39:268/269 of query aligns to 3:235/238 of 6s8gA
- binding phosphoaminophosphonic acid-adenylate ester: Y12 (= Y48), R15 (≠ L51), N37 (= N73), G40 (= G76), K41 (= K77), T42 (≠ S78), T43 (= T79), Q84 (= Q120), S136 (≠ T169), S138 (= S171), E141 (≠ Q174)
6mhzA Vanadate trapped cryo-em structure of e.Coli lptb2fg transporter (see paper)
34% identity, 86% coverage: 39:268/269 of query aligns to 3:235/235 of 6mhzA
- binding adp orthovanadate: Y12 (= Y48), N37 (= N73), G38 (= G74), G40 (= G76), K41 (= K77), T42 (≠ S78), T43 (= T79), Q84 (= Q120), S136 (≠ T169), S138 (= S171), G139 (= G172), G140 (= G173), E162 (= E195), G166 (= G199), H194 (≠ Q227)
6mbnA Lptb e163q in complex with atp (see paper)
34% identity, 86% coverage: 39:268/269 of query aligns to 4:236/241 of 6mbnA
6b89A E. Coli lptb in complex with adp and novobiocin (see paper)
34% identity, 85% coverage: 39:267/269 of query aligns to 3:234/234 of 6b89A
- binding adenosine-5'-diphosphate: Y12 (= Y48), R15 (≠ L51), V17 (≠ I53), N37 (= N73), G38 (= G74), G40 (= G76), K41 (= K77), T42 (≠ S78), T43 (= T79)
- binding magnesium ion: T42 (≠ S78), Q84 (= Q120)
- binding novobiocin: L71 (≠ P107), H72 (≠ E108), P83 (= P119), A86 (≠ E122), S87 (≠ N123), F89 (= F125), R90 (≠ A126), R91 (≠ G127), L92 (= L128), V101 (≠ G137), Q135 (≠ G168), R149 (= R182)
4p31A Crystal structure of a selenomethionine derivative of e. Coli lptb in complex with adp-magensium (see paper)
34% identity, 85% coverage: 39:267/269 of query aligns to 3:234/234 of 4p31A
6b8bA E. Coli lptb in complex with adp and a novobiocin derivative (see paper)
34% identity, 85% coverage: 39:266/269 of query aligns to 3:233/233 of 6b8bA
- binding adenosine-5'-diphosphate: Y12 (= Y48), R15 (≠ L51), V17 (≠ I53), G38 (= G74), G40 (= G76), K41 (= K77), T42 (≠ S78), T43 (= T79)
- binding (3s,5s,7s)-N-{7-[(3-O-carbamoyl-6-deoxy-5-methyl-4-O-methyl-beta-D-gulopyranosyl)oxy]-4-hydroxy-8-methyl-2-oxo-2H-1-benzopyran-3-yl}tricyclo[3.3.1.1~3,7~]decane-1-carboxamide: F89 (= F125), R90 (≠ A126), R91 (≠ G127)
4u00A Crystal structure of ttha1159 in complex with adp (see paper)
30% identity, 78% coverage: 38:246/269 of query aligns to 2:213/241 of 4u00A
1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
32% identity, 75% coverage: 44:246/269 of query aligns to 12:206/353 of 1vciA
P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
33% identity, 78% coverage: 47:256/269 of query aligns to 12:219/369 of P19566
- L86 (≠ V124) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- P160 (= P196) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- D165 (≠ A201) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
Sites not aligning to the query:
- 306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.
3d31A Modbc from methanosarcina acetivorans (see paper)
27% identity, 78% coverage: 38:246/269 of query aligns to 1:205/348 of 3d31A
Sites not aligning to the query:
P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
29% identity, 86% coverage: 38:269/269 of query aligns to 17:246/378 of P69874
- C26 (≠ G47) mutation to A: Lower ATPase activity and transport efficiency.
- F27 (≠ Y48) mutation to L: Lower ATPase activity and transport efficiency.
- F45 (≠ Y66) mutation to L: Lower ATPase activity and transport efficiency.
- C54 (≠ A75) mutation to T: Loss of ATPase activity and transport.
- L60 (≠ M81) mutation to F: Lower ATPase activity and transport efficiency.
- L76 (≠ F97) mutation to P: Lower ATPase activity and transport efficiency.
- V135 (≠ P157) mutation to M: Loss of ATPase activity and transport.
- D172 (= D194) mutation to N: Loss of ATPase activity and transport.
Sites not aligning to the query:
- 276 C→A: Lower ATPase activity and transport efficiency.
- 297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.
2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
31% identity, 78% coverage: 47:256/269 of query aligns to 11:218/374 of 2awnB
P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
31% identity, 78% coverage: 47:256/269 of query aligns to 12:219/371 of P68187
- A85 (≠ N123) mutation to M: Suppressor of EAA loop mutations in MalFG.
- K106 (vs. gap) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V114 (≠ L149) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V117 (= V152) mutation to M: Suppressor of EAA loop mutations in MalFG.
- E119 (= E154) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- A124 (≠ E160) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G137 (= G173) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
- D158 (= D194) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
Sites not aligning to the query:
- 228 R→C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 267 W→G: Normal maltose transport but constitutive mal gene expression.
- 278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
- 322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 340 G→A: Maltose transport is affected but retains ability to interact with MalT.
- 346 G→S: Normal maltose transport but constitutive mal gene expression.
- 355 F→Y: Maltose transport is affected but retains ability to interact with MalT.
3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
31% identity, 78% coverage: 47:256/269 of query aligns to 11:218/371 of 3puyA
- binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ Y48), S37 (≠ N73), G38 (= G74), C39 (≠ A75), G40 (= G76), K41 (= K77), S42 (= S78), T43 (= T79), Q81 (= Q120), R128 (≠ Q165), A132 (≠ T169), S134 (= S171), G136 (= G173), Q137 (= Q174), E158 (= E195), H191 (≠ Q227)
- binding magnesium ion: S42 (= S78), Q81 (= Q120)
3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
31% identity, 78% coverage: 47:256/269 of query aligns to 11:218/371 of 3puxA
- binding adenosine-5'-diphosphate: W12 (≠ Y48), G38 (= G74), C39 (≠ A75), G40 (= G76), K41 (= K77), S42 (= S78), T43 (= T79), R128 (≠ Q165), S134 (= S171), Q137 (= Q174)
- binding beryllium trifluoride ion: S37 (≠ N73), G38 (= G74), K41 (= K77), Q81 (= Q120), S134 (= S171), G136 (= G173), H191 (≠ Q227)
- binding magnesium ion: S42 (= S78), Q81 (= Q120)
Query Sequence
>WP_007693272.1 NCBI__GCF_000336675.1:WP_007693272.1
MSADSTDESGDGDGSPTTDESTAASTTDESSAQFPGDLLAVSALDAGYGDLQILSAVDLA
VHDGEYVTIVGPNGAGKSTVMKTVFGLTTYMGGEIRFDDDDISGLAPERIIHRGVSYVPQ
NENVFAGLSVRENLEMGAYILDSVPQYRLDWVFERFPILEERQTQRAGTLSGGQRQMLAM
GRALMLDPDLLMLDEPSAGLAPDLVDEMFDRIDAINESGTAILMVEQNAVEALSRCDRGY
VLVQGQNRFDDTGEALLGNDEVRQEFLGG
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory