SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_007693922.1 NCBI__GCF_000336675.1:WP_007693922.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

P94530 Arabinooligosaccharides transport system permease protein AraQ from Bacillus subtilis (strain 168) (see paper)
32% identity, 94% coverage: 18:281/282 of query aligns to 18:280/281 of P94530

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P94530

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D180 (= D182) mutation to A: Negative impact in the growth rate in the presence of arabinotriose.

P68183 Maltose/maltodextrin transport system permease protein MalG from Escherichia coli (strain K12) (see 2 papers)
26% identity, 71% coverage: 81:281/282 of query aligns to 87:295/296 of P68183

query
sites
P68183

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E190 (= E177) mutation E->A,C,K,L: Reduction of transport rate.
A192 (= A179) mutation A->D,S,L: Loss of transport and MalK dissociation from the membrane.
G196 (= G183) mutation to A: No effect.; mutation to P: Loss of transport and MalK dissociation from the membrane.
P209 (= P196) mutation to A: No effect.

P02916 Maltose/maltodextrin transport system permease protein MalF from Escherichia coli (strain K12) (see 4 papers)
30% identity, 37% coverage: 115:218/282 of query aligns to 335:442/514 of P02916

query
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P02916

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L372 (≠ A148) mutation to W: Growth on maltose but not on media containing either maltoheptaose or maltoheptaose plus maltose.
N376 (≠ L152) mutation N->K,H: No growth on maltose.
G380 (≠ A156) mutation G->C,S: No growth on maltose.
E401 (= E177) mutation E->A,C,K,L: Reduction of transport rate.
S403 (≠ A179) mutation S->C,D,K,L: Reduction of transport rate.
G407 (= G183) mutation G->A,P: No effect.
P420 (= P196) mutation to A: No effect.

Sites not aligning to the query:

334 L→W: Ability to transport lactose in a saturable manner.

4ki0F Crystal structure of the maltose-binding protein/maltose transporter complex in an outward-facing conformation bound to maltohexaose (see paper)
30% identity, 37% coverage: 115:218/282 of query aligns to 320:427/490 of 4ki0F

query
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4ki0F

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binding alpha-D-glucopyranose: N361 (≠ L152), G365 (≠ A156), Y368 (≠ V159), S418 (≠ L209), N422 (≠ Q213)

Sites not aligning to the query:

binding alpha-D-glucopyranose: 310

Query Sequence

>WP_007693922.1 NCBI__GCF_000336675.1:WP_007693922.1
MHEKARSEALWKFGGYGFVIAATMFVIVPIYWLLVASTLPQTEILGSAGSLPRLLPGTNF
LDNAQALAGRQNANYYQSVINSVLVATVYTVLSLILCSMSGFAFAKYEFRFKEPIFLGIL
GTLIIPINLLVIPLFLLVSNVGLSNTFAAIILPWAAYPVGIFFMRQTMQSIPDSLLEAAR
MDGASEFQLYYRVALPTVRSGMAALSVILFLFQWNLFLWPLVVLQQDKFTIPVALTTIVS
QQTPAFDQLMVAALIAIVPMLIVFVALQRHFVNGILAGAVKE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory