SitesBLAST
Comparing WP_007694028.1 NCBI__GCF_000336675.1:WP_007694028.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
6wjaA Udp-glcnac c4-epimerase mutant s121a/y146f from pseudomonas protegens in complex with udp-galnac (see paper)
43% identity, 95% coverage: 10:197/197 of query aligns to 3:197/307 of 6wjaA
- active site: A118 (≠ S120), A119 (= A121), A120 (= A122), F143 (≠ Y144), K147 (= K148)
- binding nicotinamide-adenine-dinucleotide: G7 (= G14), G10 (= G17), F11 (= F18), I12 (= I19), D31 (= D37), D32 (≠ T38), S34 (≠ G40), T35 (≠ G41), G36 (≠ Q42), A55 (≠ V58), L74 (≠ E77), A75 (= A78), A76 (= A79), S93 (≠ V96), F143 (≠ Y144), K147 (= K148), F170 (≠ Y171), F171 (= F172), I173 (≠ V174)
- binding uridine-diphosphate-n-acetylgalactosamine: V80 (= V83), A120 (= A122), N172 (= N173), G186 (= G186), V187 (= V187), F191 (= F191)
Sites not aligning to the query:
6wj9B Udp-glcnac c4-epimerase mutant s121a/y146f from pseudomonas protegens in complex with udp-glcnac (see paper)
43% identity, 95% coverage: 10:197/197 of query aligns to 4:198/308 of 6wj9B
- active site: A119 (≠ S120), A120 (= A121), A121 (= A122), F144 (≠ Y144), K148 (= K148)
- binding nicotinamide-adenine-dinucleotide: G8 (= G14), G11 (= G17), F12 (= F18), I13 (= I19), D32 (= D37), D33 (≠ T38), S35 (≠ G40), T36 (≠ G41), G37 (≠ Q42), D55 (= D57), A56 (≠ V58), L75 (≠ E77), A76 (= A78), A77 (= A79), S94 (≠ V96), A117 (= A118), A119 (≠ S120), F144 (≠ Y144), K148 (= K148), F171 (≠ Y171), F172 (= F172), I174 (≠ V174)
- binding uridine-diphosphate-n-acetylglucosamine: V81 (= V83), N173 (= N173), G187 (= G186), V188 (= V187), F192 (= F191)
Sites not aligning to the query:
6dntA Udp-n-acetylglucosamine 4-epimerase from methanobrevibacter ruminantium m1 in complex with udp-n-acetylmuramic acid (see paper)
38% identity, 96% coverage: 5:193/197 of query aligns to 1:194/310 of 6dntA
- active site: S120 (= S120), S121 (≠ A121), A122 (= A122), Y144 (= Y144), K148 (= K148), A187 (≠ G186)
- binding (2R)-2-{[(2R,3R,4R,5S,6R)-3-(acetylamino)-2-{[(S)-{[(R)-{[(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methoxy}(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]oxy}-5-hydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-4-yl]oxy}propanoic acid: A80 (= A81), S120 (= S120), S121 (≠ A121), Y144 (= Y144), F172 (= F172), N173 (= N173), A187 (≠ G186), V188 (= V187), K191 (≠ T190)
- binding nicotinamide-adenine-dinucleotide: G10 (= G14), G13 (= G17), F14 (= F18), I15 (= I19), D33 (= D37), N34 (vs. gap), L35 (vs. gap), S36 (≠ T38), S37 (≠ A39), G38 (= G40), D57 (= D57), L58 (≠ V58), L76 (≠ E77), A77 (= A78), A78 (= A79), A80 (= A81), S118 (≠ A118), S119 (= S119), Y144 (= Y144), K148 (= K148), Y171 (= Y171), V174 (= V174)
Sites not aligning to the query:
- binding (2R)-2-{[(2R,3R,4R,5S,6R)-3-(acetylamino)-2-{[(S)-{[(R)-{[(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methoxy}(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]oxy}-5-hydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-4-yl]oxy}propanoic acid: 203, 204, 205, 210, 212, 246, 269, 272
- binding zinc ion: 209, 275
1sb8A Crystal structure of pseudomonas aeruginosa udp-n-acetylglucosamine 4- epimerase complexed with udp-n-acetylgalactosamine (see paper)
34% identity, 94% coverage: 8:193/197 of query aligns to 17:216/341 of 1sb8A
- active site: S142 (= S120), S143 (≠ A121), S144 (≠ A122), Y166 (= Y144), K170 (= K148), N204 (≠ A181)
- binding nicotinamide-adenine-dinucleotide: G23 (= G14), G26 (= G17), F27 (= F18), I28 (= I19), D47 (vs. gap), N48 (≠ D37), F49 (≠ T38), T51 (≠ G40), G52 (= G41), D78 (= D57), I79 (≠ V58), Q98 (≠ E77), A100 (= A79), T117 (≠ V96), A140 (= A118), A141 (≠ S119), Y166 (= Y144), K170 (= K148), Y193 (= Y171), N195 (= N173), V196 (= V174)
- binding uridine-diphosphate-n-acetylgalactosamine: S103 (= S82), S142 (= S120), S143 (≠ A121), S144 (≠ A122), Y166 (= Y144), N195 (= N173), A209 (≠ G186), V210 (= V187), W214 (≠ F191)
Sites not aligning to the query:
1sb9A Crystal structure of pseudomonas aeruginosa udp-n-acetylglucosamine 4- epimerase complexed with udp-glucose (see paper)
34% identity, 94% coverage: 8:193/197 of query aligns to 16:215/340 of 1sb9A
- active site: S141 (= S120), S142 (≠ A121), S143 (≠ A122), Y165 (= Y144), K169 (= K148), N203 (≠ A181)
- binding nicotinamide-adenine-dinucleotide: G22 (= G14), G25 (= G17), F26 (= F18), I27 (= I19), D46 (vs. gap), N47 (≠ D37), F48 (≠ T38), T50 (≠ G40), G51 (= G41), D77 (= D57), I78 (≠ V58), Q97 (≠ E77), A99 (= A79), T116 (≠ V96), A139 (= A118), A140 (≠ S119), Y165 (= Y144), K169 (= K148), Y192 (= Y171), N194 (= N173), V195 (= V174)
- binding uridine-5'-diphosphate-glucose: S141 (= S120), Y165 (= Y144), N194 (= N173), A208 (≠ G186), V209 (= V187), W213 (≠ F191)
Sites not aligning to the query:
3aw9A Structure of udp-galactose 4-epimerase mutant
41% identity, 95% coverage: 10:196/197 of query aligns to 3:179/304 of 3aw9A
- active site: A105 (= A118), S107 (= S120), S108 (≠ A121), T109 (≠ A122), Y131 (= Y144), K135 (= K148), L166 (≠ Q179), G169 (= G186)
- binding galactose-uridine-5'-diphosphate: P69 (≠ A81), V71 (= V83), S107 (= S120), Y131 (= Y144), N160 (= N173), H168 (≠ S185), V170 (= V187), D173 (≠ T190)
- binding nicotinamide-adenine-dinucleotide: G7 (= G14), G10 (= G17), F11 (= F18), I12 (= I19), D31 (= D37), I32 (≠ T38), R46 (≠ G56), D47 (= D57), L48 (≠ V58), F65 (≠ E77), A66 (= A78), A67 (= A79), E82 (≠ V96), A105 (= A118), S106 (= S119), Y131 (= Y144), K135 (= K148), Y158 (= Y171), N160 (= N173), V161 (= V174), H168 (≠ S185)
Sites not aligning to the query:
3eheA Crystal structure of udp-glucose 4 epimerase (gale-1) from archaeoglobus fulgidus
36% identity, 95% coverage: 10:196/197 of query aligns to 2:183/290 of 3eheA
- active site: T111 (≠ S120), S112 (≠ A121), T113 (≠ A122), Y135 (= Y144), K139 (= K148)
- binding nicotinamide-adenine-dinucleotide: G6 (= G14), G9 (= G17), F10 (= F18), I11 (= I19), D29 (= D37), N30 (≠ T38), L31 (≠ A39), S32 (≠ G40), S33 (≠ G41), G34 (≠ Q42), D49 (= D57), L50 (≠ V58), I68 (≠ E77), A69 (= A78), A70 (= A79), T109 (≠ A118), Y135 (= Y144), K139 (= K148), F162 (≠ Y171), A163 (≠ F172), N164 (= N173), V165 (= V174)
2p5uA Crystal structure of thermus thermophilus hb8 udp-glucose 4-epimerase complex with NAD
39% identity, 94% coverage: 10:194/197 of query aligns to 3:193/311 of 2p5uA
- active site: T117 (≠ S120), G119 (vs. gap), A120 (= A122), Y143 (= Y144), K147 (= K148), H181 (≠ G182), G185 (= G186)
- binding nicotinamide-adenine-dinucleotide: G10 (= G17), F11 (= F18), I12 (= I19), D31 (= D37), N32 (≠ T38), L33 (≠ A39), A34 (≠ G40), T35 (≠ G41), G36 (≠ Q42), D51 (= D57), L52 (≠ V58), Q73 (≠ E77), A74 (= A78), A75 (= A79), A77 (= A81), S116 (= S119), Y143 (= Y144), K147 (= K148), V173 (= V174)
Q7BJX9 UDP-N-acetylglucosamine 4-epimerase; UDP-GalNAc 4-epimerase; EC 5.1.3.7 from Plesiomonas shigelloides (Aeromonas shigelloides) (see 2 papers)
32% identity, 94% coverage: 8:193/197 of query aligns to 20:219/345 of Q7BJX9
- GVAGFI 26:31 (≠ GGSGFI 14:19) binding NAD(+)
- DNFSTG 50:55 (vs. gap) binding NAD(+)
- DI 81:82 (≠ DV 57:58) binding NAD(+)
- QAA 101:103 (≠ EAA 77:79) binding NAD(+)
- T120 (≠ V96) binding NAD(+)
- SS 145:146 (≠ SA 120:121) binding substrate
- S147 (≠ A122) mutation to T: No effect on epimerase activity.
- Y169 (= Y144) binding NAD(+)
- K173 (= K148) binding NAD(+)
- YFN 196:198 (= YFN 171:173) binding substrate
- V199 (= V174) binding NAD(+)
- VIPK 213:216 (≠ VIST 187:190) binding substrate
Sites not aligning to the query:
- 228:230 binding substrate
- 236 S→G: No effect on epimerase activity.
- 237 binding substrate
- 271 R→G: No effect on epimerase activity.
- 302:305 binding substrate
- 307 R→A: No effect on epimerase activity.
- 308 H→A: No effect on epimerase activity.
- 309 S→Y: Abolishes epimerase activity.
3ruhA Alternative analogs as viable substrates of udp-hexose 4-epimerases
32% identity, 94% coverage: 8:193/197 of query aligns to 17:216/336 of 3ruhA
- active site: S142 (= S120), S143 (≠ A121), S144 (≠ A122), Y166 (= Y144), K170 (= K148), N204 (≠ A181)
- binding nicotinamide-adenine-dinucleotide: G23 (= G14), G26 (= G17), F27 (= F18), I28 (= I19), D47 (vs. gap), N48 (vs. gap), S50 (vs. gap), T51 (vs. gap), G52 (vs. gap), D78 (= D57), I79 (≠ V58), Q98 (≠ E77), A99 (= A78), A100 (= A79), T117 (≠ V96), A140 (= A118), A141 (≠ S119), S142 (= S120), Y166 (= Y144), K170 (= K148), Y193 (= Y171), V196 (= V174)
- binding [[(2R,3S,4R,5R)-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl][(2R,3R,4R,5R,6R)-6-(hydroxymethyl)-4,5-bis(oxidanyl)-3-(2-oxidanylidenepropyl)oxan-2-yl] hydrogen phosphate: G102 (≠ A81), S103 (= S82), S142 (= S120), S143 (≠ A121), S144 (≠ A122), Y166 (= Y144), Y193 (= Y171), N195 (= N173), A209 (≠ G186), V210 (= V187), K213 (≠ T190), W214 (≠ F191)
Sites not aligning to the query:
- binding [[(2R,3S,4R,5R)-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl][(2R,3R,4R,5R,6R)-6-(hydroxymethyl)-4,5-bis(oxidanyl)-3-(2-oxidanylidenepropyl)oxan-2-yl] hydrogen phosphate: 225, 226, 227, 234, 271, 294, 297, 298, 301
3rufA Alternative analogs as viable substrates of udp-hexose 4-epimerases
32% identity, 94% coverage: 8:193/197 of query aligns to 17:216/336 of 3rufA
- active site: S142 (= S120), S143 (≠ A121), S144 (≠ A122), Y166 (= Y144), K170 (= K148), N204 (≠ A181)
- binding nicotinamide-adenine-dinucleotide: G23 (= G14), G26 (= G17), F27 (= F18), I28 (= I19), D47 (vs. gap), N48 (vs. gap), S50 (vs. gap), T51 (vs. gap), G52 (vs. gap), D78 (= D57), I79 (≠ V58), Q98 (≠ E77), A99 (= A78), A100 (= A79), T117 (≠ V96), A140 (= A118), Y166 (= Y144), K170 (= K148), Y193 (= Y171), V196 (= V174)
- binding uridine-5'-diphosphate: N195 (= N173), A209 (≠ G186), V210 (= V187), K213 (≠ T190), W214 (≠ F191)
Sites not aligning to the query:
3lu1A Crystal structure analysis of wbgu: a udp-galnac 4-epimerase (see paper)
32% identity, 94% coverage: 8:193/197 of query aligns to 17:216/336 of 3lu1A
- active site: S142 (= S120), S143 (≠ A121), S144 (≠ A122), Y166 (= Y144), K170 (= K148), N204 (≠ A181)
- binding glycine: Q135 (≠ R114), K187 (≠ E165)
- binding nicotinamide-adenine-dinucleotide: G23 (= G14), G26 (= G17), F27 (= F18), I28 (= I19), D47 (vs. gap), N48 (vs. gap), S50 (vs. gap), T51 (vs. gap), G52 (vs. gap), D78 (= D57), I79 (≠ V58), Q98 (≠ E77), A99 (= A78), A100 (= A79), A140 (= A118), A141 (≠ S119), S142 (= S120), Y166 (= Y144), K170 (= K148), Y193 (= Y171), N195 (= N173)
- binding uridine-diphosphate-n-acetylgalactosamine: S103 (= S82), S142 (= S120), S143 (≠ A121), S144 (≠ A122), Y166 (= Y144), N195 (= N173), V210 (= V187), W214 (≠ F191)
Sites not aligning to the query:
6pnlA Structure of epimerase mth375 from the thermophilic pseudomurein- containing methanogen methanothermobacter thermautotrophicus
31% identity, 98% coverage: 4:197/197 of query aligns to 12:212/336 of 6pnlA
- active site: S133 (= S120), C135 (≠ A122), G136 (vs. gap), Y159 (= Y144), K163 (= K148)
- binding nicotinamide-adenine-dinucleotide: G22 (= G14), G25 (= G17), C26 (≠ F18), D46 (= D37), N47 (≠ T38), L48 (≠ A39), S49 (≠ G40), S50 (≠ G41), S51 (≠ Q42), D68 (= D57), I69 (≠ V58), L89 (≠ E77), A91 (= A79), F93 (≠ A81), V108 (= V96), S131 (≠ A118), S133 (= S120), Y159 (= Y144), K163 (= K148), F186 (≠ Y171), N188 (= N173), V189 (= V174), R200 (≠ S185)
- binding uridine-5'-diphosphate: N188 (= N173), N201 (≠ G186), V202 (= V187), F206 (= F191)
Sites not aligning to the query:
4zrnA Crystal structure of udp-glucose 4-epimerase (tm0509) with udp-glucose from hyperthermophilic eubacterium thermotoga maritima (see paper)
34% identity, 94% coverage: 9:194/197 of query aligns to 2:193/309 of 4zrnA
- active site: T117 (≠ S120), G119 (vs. gap), A120 (= A122), Y143 (= Y144), K147 (= K148), Y181 (≠ G182), G185 (= G186)
- binding nicotinamide-adenine-dinucleotide: G7 (= G14), G10 (= G17), F11 (= F18), I12 (= I19), D31 (= D37), N32 (≠ T38), S34 (≠ G40), S35 (≠ G41), G36 (≠ Q42), S51 (≠ D57), I52 (≠ V58), L73 (≠ E77), A74 (= A78), A75 (= A79), T92 (≠ V96), S115 (≠ A118), S116 (= S119), Y143 (= Y144), K147 (= K148), Y170 (= Y171), V173 (= V174)
- binding uridine-5'-diphosphate-glucose: T117 (≠ S120), G119 (vs. gap), A120 (= A122), Y143 (= Y144), N172 (= N173), G185 (= G186), V186 (= V187)
Sites not aligning to the query:
6pmhA Structure of epimerase mth375 from the thermophilic pseudomurein- containing methanogen methanothermobacter thermautotrophicus
31% identity, 98% coverage: 4:197/197 of query aligns to 6:206/330 of 6pmhA
- active site: S127 (= S120), C129 (≠ A122), G130 (vs. gap), Y153 (= Y144), K157 (= K148)
- binding adenosine monophosphate: G16 (= G14), G19 (= G17), D40 (= D37), N41 (≠ T38), S43 (≠ G40), S44 (≠ G41), S45 (≠ Q42), D62 (= D57), I63 (≠ V58), A84 (= A78), F87 (≠ A81), R194 (≠ S185)
- binding uridine-5'-diphosphate: C129 (≠ A122), N182 (= N173), N195 (≠ G186), V196 (= V187), F200 (= F191)
Sites not aligning to the query:
4twrA Structure of udp-glucose 4-epimerase from brucella abortus
39% identity, 85% coverage: 9:176/197 of query aligns to 3:173/325 of 4twrA
- active site: S117 (= S120), C118 (≠ A121), A119 (= A122), Y141 (= Y144), K145 (= K148)
- binding nicotinamide-adenine-dinucleotide: G8 (= G14), G11 (= G17), F12 (= F18), I13 (= I19), D32 (vs. gap), N33 (vs. gap), L34 (vs. gap), S35 (≠ T38), T36 (≠ A39), G37 (= G40), D51 (= D57), I52 (≠ V58), F73 (≠ E77), A74 (= A78), A75 (= A79), N92 (≠ V96), S115 (≠ A118), K145 (= K148), Y168 (= Y171), A171 (≠ V174)
Sites not aligning to the query:
7ysmA Crystal structure of udp-glucose 4-epimerase (rv3634c) co-crystallized with udp-n-acetylglucosamine from mycobacterium tuberculosis
36% identity, 92% coverage: 11:191/197 of query aligns to 4:193/311 of 7ysmA
- binding nicotinamide-adenine-dinucleotide: G7 (= G14), G10 (= G17), F11 (= F18), I12 (= I19), D31 (= D37), N32 (≠ T38), T35 (≠ G40), G36 (= G41), D56 (= D52), I57 (≠ V53), L77 (≠ E77), A78 (= A78), A79 (= A79), I81 (≠ A81), T119 (vs. gap), Y146 (= Y144), K150 (= K148), P173 (≠ Y171), N175 (= N173), V176 (= V174)
- binding uridine-diphosphate-n-acetylgalactosamine: I81 (≠ A81), R84 (≠ E84), S121 (= S119), G123 (≠ A121), S124 (≠ A122), Y146 (= Y144), A174 (≠ F172), N175 (= N173), G188 (= G186), V189 (= V187), F193 (= F191)
Sites not aligning to the query:
7ystA Crystal structure of udp-glucose 4-epimerase (rv3634c) in complex with both udp-glucose and udp-galactose in chain b from mycobacterium tuberculosis
36% identity, 92% coverage: 11:191/197 of query aligns to 4:193/312 of 7ystA