SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_007694040.1 NCBI__GCF_000336675.1:WP_007694040.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6rl5G The first crystal structure of the daba aminotransferase ectb in the ectoine biosynthesis pathway of the model organism chromohalobacter salexigens dsm 3034 (see paper)
35% identity, 97% coverage: 8:448/454 of query aligns to 1:421/422 of 6rl5G

query
sites
6rl5G

N

Q

S

T

E

Q

V

I

L

L

T

E

R

R

Q

M

S

-

E

-

R

-

E

E

S

S

N

E

A

V

R

R

T

T

Y

Y

P
x
S

R

R

H

S

L

F

P

P

F

T

A

V

I

F

R

T

E

E

A

A

T

K

G

G

V

A

T

R

V

L

T

H

D

A

M

E

D

D

G

G

N

N

E

Q

Y

Y

Y

I

D

D

C

F

L

L

A

A

G

G

A

A

G

G

T

T

L

L

A

N

L

Y

G

G

H

H

N

N

H

H

P

P

R

K

V

L

V

K

E

Q

A

A

M

L

E

A

R

D

V

Y

I

I

D

A

E

S

D

D

R

G

P

I

I

V

H

H

T

G

L

L

D

D

I

M

S

W

T

S

P

A

T

A

K

K

E

R

R

D

F

Y

V

L

D

E

S

T

L

L

F

E

E

E

S

V

L

I

P

L

D

K

E

P

F

R

T

G

D

L

R

D

A

Y

K

K

I

V

Q

H

F

L

C

P

S

G

P

P

A

T

G
|
G

T
|
T

D

N

A

A

V

V

E

E

A

A

A

A

L

I

K

R

L

L

V

A

K

R

T

N

A

A

T

K

G

G

N

R

R

H

S

N

V

I

L

V

G

T

F

F

Q

T

G

N

A

G

Y
x
F

H
|
H

G

G

M

V

T

T

S

M

G

G

A

A

L

L

G

A

L

T

M

T

G

G

D

N

V

R

D

K

V

F

K

R

-

E

-

A

-

T

G

G

T

G

L

I

P

P

A

T

S

Q

T

G

G

-

E

-

V

-

H

-

H

-

L

-

P

-

Y

-

P

-

S

A

N

S

Y

F

R

M

P

P

P

F

F

D

G

G

V

Y

G

M

G

G

E

E

E

G

G

V

H

D

R

T

I

L

A

S

S

-

R

-

Y

Y

V

F

R

E

N

K

L

L

L

L

A

G

D

D

S

N

K

S

S

G

G

G

I

L

T

D

D

V

P

P

A

A

G

A

M

V

I

I

L

I

E

E

P

T

I

V

Q

Q

G

G

E

E

G

G

G

G

S

I

V

N

P

P

A

A

P

G

D

I

E

P

W

W

L

L

R

Q

E

R

M

L

R

E

R

K

I

I

T

C

R

R

E

D

H

H

D

D

I

M

P

L

L

L

I

I

L

V

D
|
D

E

D

I
|
I

Q
|
Q

T

A

G

G

L

C

G

G

R

R

T

T

G

G

E

K

T

F

Y

F

A

S

F

F

E

E

H

H

A

A

D

G

I

I

V

T

P

P

D

D

V

I

V

V

T

T

L

N

S

S

K
|
K

A

S

I

L

G

S

G

G

-

F

G

G

L

L

P

P

L

F

A

A

V

H

V

V

V

L

Y

M

D

R

E

P

S

E

L

L

D

D

V

I

W

W

E

K

P

P

G

G

A

Q

H

Y

A

N

G
|
G

T
|
T

F

F

R

R

G

G

N

F

Q

N

L

L

A

A

M

F

A

V

A

T

G

A

E

A

A

A

T

A

I

M

D

R

H

H

V

F

V

W

E

S

N

D

D

D

L

T

A

F

E

E

H

R

A

D

A

V

D

Q

-

R

V

K

G

G

R

R

R

V

L

V

R

E

E

D

A

R

F

F

E

Q

A

K

T

L

A

A

E

S

R

F

F

M

E

T

A

E

V

K

G

G

-

H

-

P

-

A

D

S

V

E

R

R

G

G

R

R

G

G

L

L

M

M

L

R

G

G

V

L

E

D

F

-

V

-

D

-

H

-

G

-

A

-

E

-

W

-

Q

-

G

-

P

-

G

-

P

-

H

-

A

V

P

G

D

D

G

G

D

D

F

M

A

A

E

D

A

K

V

I

Q

T

A

A

A

Q

C

A

F

F

E

K

R

N

G

G

L

L

V

I

V

I

E

E

R

T

G

S

G

G

R

H

G

S

D

G

A

Q

T

V

A

I

R

K

F

C

L

L

P

C

P

P

L

L

I

T

V

I

T

T

R

D

A

E

Q

D

V

L

D

V

E

G

I

G

A

L

T

D

I

I

F

L

E

E

E

Q

A

S

V

V

A

K

A

E

V

V

A

F

A

G

E

Q

active site: S16 (≠ P26), F137 (≠ Y147), D237 (= D255), K266 (= K284)
binding pyridoxal-5'-phosphate: G110 (= G120), T111 (= T121), F137 (≠ Y147), H138 (= H148), D237 (= D255), I239 (= I257), Q240 (= Q258), K266 (= K284), G294 (= G311), T295 (= T312)

O50131 Ornithine aminotransferase; Orn-AT; Ornithine delta-aminotransferase; EC 2.6.1.13 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see paper)
36% identity, 93% coverage: 26:446/454 of query aligns to 33:446/454 of O50131

query
sites
O50131

P

P

R

N

H

E

L

Y

P

F

F

L

A

V

I

I

R

E

E

R

A

A

T

E

G

G

V

V

T

Y

V

W

T

I

D

D

M

V

D

D

G

G

N

N

E

V

Y

L

Y

L

D

D

C

F

L

S

A

S

G

G

A

I

G

G

T

V

L

M

A

N

L

V

G

G

H

L

N

R

H

N

P

P

R

K

V

V

V

I

E

E

A

A

M

I

E

K

R

K

V

Q

I

L

D

D

E

L

D

V

R

L

P

H

I

A

H

A

T

G

L
x
T

D
|
D

I

Y

S

Y

T

N

P

P

T

Y

K

Q

E

V

R

E

F

L

V

A

D

K

S

K

L

L

F

V

E

E

S

I

L

A

P

P

D

G

E

D

F

I

T

E

D

R

R

K

A

V

K

-

I

-

Q

-

F

F

C

L

S

S

P

N

A

S

G
|
G

T
|
T

D

E

A

A

V

N

E

E

A

A

A

A

L

L

K

K

L

I

V

A

K

K

T

W

A

S

T

T

G

N

N

R

R

K

S

M

V

F

L

I

G

A

F

F

Q

I

G

G

A

A

Y

F

H

H

G

G

M

R

T

T

S

H

G

G

A

T

L

M

G

S

L

L

M

T

G

A

D

S

V

K

D

P

V

V

K

Q

G

R

T

S

-

R

-

M

L

F

P

P

A

T

S

M

T

P

G

G

E

V

V

V

H

H

H

-

L

V

P

P

Y

Y

P

P

S

N

N

P

Y

Y

R

R

P

N

P

P

F

W

G

G

V

I

G

D

G

G

E

Y

E

E

G

N

-

P

-

D

-

E

-

L

-

I

H

N

R

R

I

V

A

I

S

D

R

-

Y

Y

V

I

R

E

N

E

L

Y

L

L

A

F

D

E

S

H

K

Y

S

V

G

P

I

A

T

E

D

E

P

V

A

A

G

G

M

I

I

F

L

F

E

E

P

P

I

I

Q

Q

G

G

E

E

G

G

G

G

S

Y

V

V

P

V

A

P

P

P

D

K

E

N

W

F

L

F

R

K

E

E

M

L

R

K

R

K

I

L

T

A

R

D

E

K

H

H

D

G

I

I

P

L

L

L

I

I

L

D

D

D

E

E

I

V

Q
|
Q

T

M

G

G

L

M

G

G

R

R

T

T

G

G

E

R

T

M

Y

W

A

A

F

I

E

E

H

H

A

F

D

D

I

I

V

V

P

P

D

D

V

I

V

V

T

T

L

V

S

A

K
|
K

A

A

I

L

G

G

G

G

G

G

L

I

P

P

L

I

A

G

V

A

V

T

V

I

Y

F

D

R

E

A

S

D

L

L

D

D

V

F

W

G

E

V

P

S

G

G

A

V

H

H

A

S

G

N

T
|
T

F

F

R

G

G

G

N

N

Q

T

L

V

A

A

M

A

A

A

A

A

G

A

E

L

A

A

T

V

I

I

D

E

H

E

V

-

V

L

E

Q

N

N

D

G

L

L

A

I

E

E

H

N

A

A

A

Q

D

K

V

L

G

E

R

P

R

L

L

F

R

R

E

E

A

R

F

L

E

E

A

E

T

M

A

K

E

E

R

K

F

Y

E

E

A

I

V

I

G

G

D

D

V

V

R

R

G

G

R

L

G

G

L

L

M

A

L

W

G

G

V

V

E

E

F

F

V

V

D

K

H

D

G

-

A

-

E

-

W

-

Q

-

G

-

P

-

G

-

P

-

H

-

A

-

P

-

D

-

G

-

D

-

F

-

A

R

E

K

A

T

V

K

Q

E

A

Y

A

A

C

T

F

K

E

E

R

R

G

G

L

E

V

I

V

V

-

V

-

E

-

A

-

L

E

K

R

R

G

G

-

L

-

A

-

L

-

L

G

G

R

C

G

G

D

K

A

S

T

A

A

I

R

R

F

L

L

I

P

P

P

P

L

L

I

I

V

I

T

S

R

E

A

E

Q

E

V

A

D

K

E

M

I

G

A

L

T

D

I

I

F

F

E

E

E

E

A

A

V

I

A

K

A

V

V

V

A

S

T92 (≠ L85) mutation to V: Slightly increases the specific activity. Increases KM for L-ornithine.
D93 (= D86) mutation to L: Reduces the specific activity. Increases KM for L-ornithine.
G124 (= G120) binding pyridoxal 5'-phosphate
T125 (= T121) binding pyridoxal 5'-phosphate
Q267 (= Q258) binding pyridoxal 5'-phosphate
K293 (= K284) modified: N6-(pyridoxal phosphate)lysine
T321 (= T312) binding pyridoxal 5'-phosphate

7vo1A Structure of aminotransferase-substrate complex (see paper)
36% identity, 93% coverage: 26:446/454 of query aligns to 31:444/452 of 7vo1A

query
sites
7vo1A

P

P

R

N

H

E

L

Y

P

F

F

L

A

V

I

I

R

E

E

R

A

A

T

E

G

G

V

V

T

Y

V

W

T

I

D

D

M

V

D

D

G

G

N

N

E

V

Y

L

Y

L

D

D

C

F

L

S

A

S

G

G

A
x
I

G

G

T

V

L

M

A

N

L

V

G

G

H

L

N

R

H

N

P

P

R

K

V

V

V

I

E

E

A

A

M

I

E

K

R

K

V

Q

I

L

D

D

E

L

D

V

R

L

P

H

I

A

H

A

T

G

L

T

D

D

I

Y

S

Y

T

N

P

P

T

Y

K

Q

E

V

R

E

F

L

V

A

D

K

S

K

L

L

F

V

E

E

S

I

L

A

P

P

D

G

E

D

F

I

T

E

D

R

R

K

A

V

K

-

I

-

Q

-

F

F

C

L

S

S

P

N

A
x
S

G
|
G

T
|
T

D

E

A

A

V

N

E

E

A

A

A

A

L

L

K

K

L

I

V

A

K

K

T

W

A

S

T

T

G

N

N

R

R

K

S

M

V

F

L

I

G

A

F

F

Q

I

G

G

A

A

Y
x
F

H
|
H

G

G

M
x
R

T

T

S

H

G

G

A

T

L

M

G

S

L

L

M

T

G

A

D

S

V

K

D

P

V

V

K

Q

G

R

T

S

-

R

-

M

L

F

P

P

A

T

S

M

T

P

G

G

E

V

V

V

H

H

H

-

L

V

P

P

Y

Y

P

P

S

N

N

P

Y

Y

R

R

P

N

P

P

F

W

G

G

V

I

G

D

G

G

E

Y

E

E

G

N

-

P

-

D

-

E

-

L

-

I

H

N

R

R

I

V

A

I

S

D

R

-

Y

Y

V

I

R

E

N

E

L

Y

L

L

A

F

D

E

S

H

K

Y

S

V

G

P

I

A

T

E

D

E

P

V

A

A

G

G

M

I

I

F

L

F

E

E

P

P

I

I

Q

Q

G

G

E
|
E

G

G

G

G

S

Y

V

V

P

V

A

P

P

P

D

K

E

N

W

F

L

F

R

K

E

E

M

L

R

K

R

K

I

L

T

A

R

D

E

K

H

H

D

G

I

I

P

L

L

L

I

I

L

D

D
|
D

E

E

I
x
V

Q
|
Q

T

M

G

G

L

M

G

G

R

R

T

T

G

G

E

R

T

M

Y

W

A

A

F

I

E

E

H

H

A

F

D

D

I

I

V

V

P

P

D

D

V

I

V

V

T

T

L

V

S

A

K
|
K

A

A

I

L

G

G

G

G

G

G

L

I

P

P

L

I

A

G

V

A

V

T

V

I

Y

F

D

R

E

A

S

D

L

L

D

D

V

F

W

G

E

V

P

S

G

G

A

V

H

H

A

S

G
x
N

T
|
T

F

F

R

G

G

G

N

N

Q

T

L

V

A

A

M

A

A

A

A

A

G

A

E

L

A

A

T

V

I

I

D

E

H

E

V

-

V

L

E

Q

N

N

D

G

L

L

A

I

E

E

H

N

A

A

A

Q

D

K

V

L

G

E

R

P

R

L

L

F

R

R

E

E

A

R

F

L

E

E

A

E

T

M

A

K

E

E

R

K

F

Y

E

E

A

I

V

I

G

G

D

D

V

V

R

R

G

G

R

L

G

G

L

L

M

A

L

W

G

G

V

V

E

E

F

F

V

V

D

K

H

D

G

-

A

-

E

-

W

-

Q

-

G

-

P

-

G

-

P

-

H

-

A

-

P

-

D

-

G

-

D

-

F

-

A

R

E

K

A

T

V

K

Q

E

A

Y

A

A

C

T

F

K

E

E

R

R

G

G

L

E

V

I

V

V

-

V

-

E

-

A

-

L

E

K

R

R

G

G

-

L

-

A

-

L

-

L

G

G

R

C

G

G

D

K

A

S

T

A

A

I

R
|
R

F

L

L

I

P

P

P

P

L

L

I

I

V

I

T

S

R

E

A

E

Q

E

V

A

D

K

E

M

I

G

A

L

T

D

I

I

F

F

E

E

E

E

A

A

V

I

A

K

A

V

V

V

A

S

binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: I61 (≠ A56), S121 (≠ A119), G122 (= G120), T123 (= T121), F149 (≠ Y147), H150 (= H148), R152 (≠ M150), E234 (= E227), D262 (= D255), V264 (≠ I257), Q265 (= Q258), K291 (= K284), N318 (≠ G311), T319 (= T312), R417 (= R419)

7vntA Structure of aminotransferase-substrate complex (see paper)
36% identity, 93% coverage: 26:446/454 of query aligns to 31:444/452 of 7vntA

query
sites
7vntA

P

P

R

N

H

E

L

Y

P

F

F

L

A

V

I

I

R

E

E

R

A

A

T

E

G

G

V

V

T

Y

V

W

T

I

D

D

M

V

D

D

G

G

N

N

E

V

Y

L

Y

L

D

D

C

F

L

S

A

S

G

G

A

I

G

G

T

V

L

M

A

N

L

V

G

G

H

L

N

R

H

N

P

P

R

K

V

V

V

I

E

E

A

A

M

I

E

K

R

K

V

Q

I

L

D

D

E

L

D

V

R

L

P

H

I

A

H

A

T

G

L

T

D

D

I

Y

S

Y

T

N

P

P

T

Y

K

Q

E

V

R

E

F

L

V

A

D

K

S

K

L

L

F

V

E

E

S

I

L

A

P

P

D

G

E

D

F

I

T

E

D

R

R

K

A

V

K

-

I

-

Q

-

F

F

C

L

S

S

P

N

A

S

G
|
G

T
|
T

D

E

A

A

V

N

E

E

A

A

A

A

L

L

K

K

L

I

V

A

K

K

T

W

A

S

T

T

G

N

N

R

R

K

S

M

V

F

L

I

G

A

F

F

Q

I

G

G

A

A

Y
x
F

H
|
H

G

G

M
x
R

T

T

S

H

G

G

A

T

L

M

G

S

L

L

M

T

G

A

D

S

V

K

D

P

V

V

K

Q

G

R

T

S

-

R

-

M

L

F

P

P

A

T

S

M

T

P

G

G

E

V

V

V

H

H

H

-

L

V

P

P

Y

Y

P

P

S

N

N

P

Y

Y

R

R

P

N

P

P

F

W

G

G

V

I

G

D

G

G

E

Y

E

E

G

N

-

P

-

D

-

E

-

L

-

I

H

N

R

R

I

V

A

I

S

D

R

-

Y

Y

V

I

R

E

N

E

L

Y

L

L

A

F

D

E

S

H

K

Y

S

V

G

P

I

A

T

E

D

E

P

V

A

A

G

G

M

I

I

F

L

F

E
|
E

P

P

I

I

Q

Q

G

G

E
|
E

G

G

G

G

S

Y

V

V

P

V

A

P

P

P

D

K

E

N

W

F

L

F

R

K

E

E

M

L

R

K

R

K

I

L

T

A

R

D

E

K

H

H

D

G

I

I

P

L

L

L

I

I

L

D

D
|
D

E

E

I
x
V

Q
|
Q

T

M

G

G

L

M

G

G

R

R

T

T

G

G

E

R

T

M

Y

W

A

A

F

I

E

E

H

H

A

F

D

D

I

I

V

V

P

P

D

D

V

I

V

V

T

T

L

V

S

A

K
|
K

A

A

I

L

G

G

G

G

G

G

L

I

P

P

L

I

A

G

V

A

V

T

V

I

Y

F

D

R

E

A

S

D

L

L

D

D

V

F

W

G

E

V

P

S

G

G

A

V

H

H

A

S

G

N

T

T

F

F

R

G

G

G

N

N

Q

T

L

V

A

A

M

A

A

A

A

A

G

A

E

L

A

A

T

V

I

I

D

E

H

E

V

-

V

L

E

Q

N

N

D

G

L

L

A

I

E

E

H

N

A

A

A

Q

D

K

V

L

G

E

R

P

R

L

L

F

R

R

E

E

A

R

F

L

E

E

A

E

T

M

A

K

E

E

R

K

F

Y

E

E

A

I

V

I

G

G

D

D

V

V

R

R

G

G

R

L

G

G

L

L

M

A

L

W

G

G

V

V

E

E

F

F

V

V

D

K

H

D

G

-

A

-

E

-

W

-

Q

-

G

-

P

-

G

-

P

-

H

-

A

-

P

-

D

-

G

-

D

-

F

-

A

R

E

K

A

T

V

K

Q

E

A

Y

A

A

C

T

F

K

E

E

R

R

G

G

L

E

V

I

V

V

-

V

-

E

-

A

-

L

E

K

R

R

G

G

-

L

-

A

-

L

-

L

G

G

R

C

G

G

D

K

A

S

T

A

A

I

R

R

F

L

L

I

P

P

P

P

L

L

I

I

V

I

T

S

R

E

A

E

Q

E

V

A

D

K

E

M

I

G

A

L

T

D

I

I

F

F

E

E

E

E

A

A

V

I

A

K

A

V

V

V

A

S

binding L-ornithine: F149 (≠ Y147), R152 (≠ M150), E234 (= E227), K291 (= K284)
binding pyridoxal-5'-phosphate: G122 (= G120), T123 (= T121), F149 (≠ Y147), H150 (= H148), E229 (= E222), D262 (= D255), V264 (≠ I257), Q265 (= Q258), K291 (= K284)

7vnoA Structure of aminotransferase (see paper)
36% identity, 93% coverage: 26:446/454 of query aligns to 31:444/452 of 7vnoA

query
sites
7vnoA

P

P

R

N

H

E

L

Y

P

F

F

L

A

V

I

I

R

E

E

R

A

A

T

E

G

G

V

V

T

Y

V

W

T

I

D

D

M

V

D

D

G

G

N

N

E

V

Y

L

Y

L

D

D

C

F

L

S

A

S

G

G

A

I

G

G

T

V

L

M

A

N

L

V

G

G

H

L

N

R

H

N

P

P

R

K

V

V

V

I

E

E

A

A

M

I

E

K

R

K

V

Q

I

L

D

D

E

L

D

V

R

L

P

H

I

A

H

A

T

G

L

T

D

D

I

Y

S

Y

T

N

P

P

T

Y

K

Q

E

V

R

E

F

L

V

A

D

K

S

K

L

L

F

V

E

E

S

I

L

A

P

P

D

G

E

D

F

I

T

E

D

R

R

K

A

V

K

-

I

-

Q

-

F

F

C

L

S

S

P

N

A

S

G
|
G

T
|
T

D

E

A

A

V
x
N

E

E

A

A

A

A

L

L

K

K

L

I

V

A

K

K

T

W

A

S

T

T

G

N

N

R

R

K

S

M

V

F

L

I

G

A

F

F

Q

I

G

G

A

A

Y
x
F

H
|
H

G

G

M

R

T

T

S

H

G

G

A

T

L

M

G

S

L

L

M

T

G

A

D

S

V

K

D

P

V

V

K

Q

G

R

T

S

-

R

-

M

L

F

P

P

A

T

S

M

T

P

G

G

E

V

V

V

H

H

H

-

L

V

P

P

Y

Y

P

P

S

N

N

P

Y

Y

R

R

P

N

P

P

F

W

G

G

V

I

G

D

G

G

E

Y

E

E

G

N

-

P

-

D

-

E

-

L

-

I

H

N

R

R

I

V

A

I

S

D

R

-

Y

Y

V

I

R

E

N

E

L

Y

L

L

A

F

D

E

S

H

K

Y

S

V

G

P

I

A

T

E

D

E

P

V

A

A

G

G

M

I

I

F

L

F

E
|
E

P

P

I

I

Q

Q

G

G

E

E

G

G

G

G

S

Y

V

V

P

V

A

P

P

P

D

K

E

N

W

F

L

F

R

K

E

E

M

L

R

K

R

K

I

L

T

A

R

D

E

K

H

H

D

G

I

I

P

L

L

L

I

I

L

D

D
|
D

E

E

I
x
V

Q
|
Q

T

M

G

G

L

M

G

G

R

R

T

T

G

G

E

R

T

M

Y

W

A

A

F

I

E

E

H

H

A

F

D

D

I

I

V

V

P

P

D

D

V

I

V

V

T

T

L

V

S

A

K
|
K

A

A

I

L

G

G

G

G

G

G

L

I

P

P

L

I

A

G

V

A

V

T

V

I

Y

F

D

R

E

A

S

D

L

L

D

D

V

F

W

G

E

V

P

S

G

G

A

V

H

H

A

S

G

N

T

T

F

F

R

G

G

G

N

N

Q

T

L

V

A

A

M

A

A

A

A

A

G

A

E

L

A

A

T

V

I

I

D

E

H

E

V

-

V

L

E

Q

N

N

D

G

L

L

A

I

E

E

H

N

A

A

A

Q

D

K

V

L

G

E

R

P

R

L

L

F

R

R

E

E

A

R

F

L

E

E

A

E

T

M

A

K

E

E

R

K

F

Y

E

E

A

I

V

I

G

G

D

D

V

V

R

R

G

G

R

L

G

G

L

L

M

A

L

W

G

G

V

V

E

E

F

F

V

V

D

K

H

D

G

-

A

-

E

-

W

-

Q

-

G

-

P

-

G

-

P

-

H

-

A

-

P

-

D

-

G

-

D

-

F

-

A

R

E

K

A

T

V

K

Q

E

A

Y

A

A

C

T

F

K

E

E

R

R

G

G

L

E

V

I

V

V

-

V

-

E

-

A

-

L

E

K

R

R

G

G

-

L

-

A

-

L

-

L

G

G

R

C

G

G

D

K

A

S

T

A

A

I

R

R

F

L

L

I

P

P

P

P

L

L

I

I

V

I

T

S

R

E

A

E

Q

E

V

A

D

K

E

M

I

G

A

L

T

D

I

I

F

F

E

E

E

E

A

A

V

I

A

K

A

V

V

V

A

S

binding pyridoxal-5'-phosphate: G122 (= G120), T123 (= T121), N126 (≠ V124), F149 (≠ Y147), H150 (= H148), E229 (= E222), D262 (= D255), V264 (≠ I257), Q265 (= Q258), K291 (= K284)

1wkhA Acetylornithine aminotransferase from thermus thermophilus hb8
34% identity, 95% coverage: 15:447/454 of query aligns to 6:387/387 of 1wkhA

query
sites
1wkhA

Q

E

S

A

E

E

R

K

E

T

S

L

N

D

A
x
S

R

G

T

V

Y

Y

P

N

R

K

H

H

L

D

P

L

F

L

A

I

I

V

R

R

E

-

A

G

T

Q

G

G

V

A

T

R

V

V

T

W

D

D

M

A

D

E

G

G

N

N

E

E

Y

Y

Y

I

D

D

C

C

L

V

A

G

G

G

A
x
Y

G

G

T

V

L

A

A

N

L

L

G

G

H

H

N

G

H

N

P

P

R

E

V

V

V

V

E

E

A

A

M

V

E

K

R

R

V

Q

I

A

D

E

E

T

D

L

R

M

P

A

I

M

H

P

T

Q

L

-

D

T

I

L

S

P

T

T

P

P

T

M

K

R

E

G

R

E

F

F

V

Y

D

R

S

T

L

L

F

T

E

A

S

I

L

L

P

P

D

P

E

E

F

L

T

N

D

R

R

-

A

-

K

-

I

-

Q

V

F

F

C

P

S

V

P

N

A
x
S

G
|
G

T
|
T

D

E

A

A

V

N

E

E

A

A

A

A

L

L

K

K

L

F

V

A

K

R

T

A

A

H

T

T

G

G

N

R

R

K

S

K

V

F

L

V

G

A

F

A

Q

M

G

R

A

G

Y
x
F

H
x
S

G

G

M

R

T

T

S

M

G

G

A

S

L

L

G

S

L

V

M

T

G

W

D

E

V

P

D

K

V

Y

K

R

G

E

T

P

L

F

P

L

A

P

S

L

T

V

G

E

E

P

V

V

H

E

H

F

L

I

P

P

Y

Y

P

-

S

-

N

-

Y

-

R

-

P

-

P

-

F

-

G

-

V

-

G

-

G

-

E

-

E

-

G

-

H

-

R

-

I

-

A

-

S

-

R

-

Y

-

V

-

R

N

N

D

L

V

L

E

A

A

D

L

S

K

K

R

S

A

G

V

I

D

T

E

D

E

P

T

A

A

G

A

M

V

I

I

L

L

E
|
E

P

P

I

V

Q

Q

G

G

E
|
E

G

G

G

G

S

V

V

R

P

P

A

A

P

T

D

P

E

E

W

F

L

L

R

R

E

A

M

A

R

R

R

E

I

I

T

T

R

Q

E

E

H

K

D

G

I

A

P

L

L

L

I

I

L

L

D
|
D

E

E

I
|
I

Q
|
Q

T

T

G

G

L

M

G

G

R

R

T

T

G

G

E

K

T

R

Y

F

A

A

F

F

E

E

H

H

A

F

D

G

I

I

V

V

P

P

D

D

V

I

V

L

T

T

L

L

S

A

K
|
K

A

A

I

L

G

G

G

G

G

G

L

V

P

P

L

L

A

G

V

V

V

A

V

V

Y

M

D

R

E

E

S

E

L

V

D

A

V

R

W

S

E

M

P

P

-

K

G

G

A

G

H

H

A

G

G

T

T
|
T

F

F

R

G

G

G

N

N

Q

P

L

L

A

A

M

M

A

A

A

A

G

G

E

V

A

A

T

A

I

I

D

R

H

Y

V

L

V

E

E

R

N

T

D

R

L

L

A

W

E

E

H

R

A

A

A

A

D

E

V

L

G

G

R

P

R

W

L

F

R

M

E

E

A

K

F

L

E

R

A

A

T

I

A

P

E

S

R

-

F

-

E

P

A

K

V

I

G

R

D

E

V

V

R

R

G

G

R

M

G

G

L

L

M

M

L

V

G

G

V

L

E

E

F

L

V

K

D

E

H

K

G

A

A

A

E

P

W

Y

Q

-

G

-

P

-

G

-

P

-

H

-

A

-

P

-

D

-

G

-

D

-

F

-

A

-

E

-

A

-

V

-

Q

-

A

I

A

A

C

R

F

L

E

E

R

K

G

E

L

H

V

R

V

V

E

L

R

A

G

L

G

Q

R

A

G

G

D

P

A

T

T

V

A

I

R
|
R

F

F

L

L

P

P

P

P

L

L

I

V

V

I

T

E

R

K

A

E

Q

D

V

L

D

E

E

R

I

V

A

V

T

-

I

-

F

-

E

-

E

E

A

A

V

V

A

R

A

A

V

V

A

L

A

A

active site: S13 (≠ A22), F132 (≠ Y147), E184 (= E222), D217 (= D255), Q220 (= Q258), K246 (= K284), T275 (= T312), R363 (= R419)
binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: Y46 (≠ A56), S104 (≠ A119), G105 (= G120), T106 (= T121), F132 (≠ Y147), S133 (≠ H148), E184 (= E222), E189 (= E227), D217 (= D255), I219 (= I257), K246 (= K284), R363 (= R419)

1wkgA Acetylornithine aminotransferase from thermus thermophilus hb8
34% identity, 95% coverage: 15:447/454 of query aligns to 6:387/387 of 1wkgA

query
sites
1wkgA

Q

E

S

A

E

E

R

K

E

T

S

L

N

D

A
x
S

R

G

T

V

Y
|
Y

P

N

R

K

H

H

L

D

P

L

F

L

A

I

I

V

R

R

E

-

A

G

T

Q

G

G

V

A

T

R

V

V

T

W

D

D

M

A

D

E

G

G

N

N

E

E

Y

Y

Y

I

D

D

C

C

L

V

A

G

G

G

A
x
Y

G

G

T

V

L

A

A

N

L

L

G

G

H

H

N

G

H

N

P

P

R

E

V

V

V

V

E

E

A

A

M

V

E

K

R

R

V

Q

I

A

D

E

E

T

D

L

R

M

P

A

I

M

H

P

T

Q

L

-

D

T

I

L

S

P

T

T

P

P

T

M

K

R

E

G

R

E

F

F

V

Y

D

R

S

T

L

L

F

T

E

A

S

I

L

L

P

P

D

P

E

E

F

L

T

N

D

R

R

-

A

-

K

-

I

-

Q

V

F

F

C

P

S

V

P

N

A

S

G
|
G

T
|
T

D

E

A

A

V

N

E

E

A

A

A

A

L

L

K

K

L

F

V

A

K

R

T

A

A

H

T

T

G

G

N

R

R

K

S

K

V

F

L

V

G

A

F

A

Q

M

G

R

A

G

Y
x
F

H
x
S

G

G

M
x
R

T

T

S

M

G

G

A

S

L

L

G

S

L

V

M

T

G

W

D

E

V

P

D

K

V

Y

K

R

G

E

T

P

L

F

P

L

A

P

S

L

T

V

G

E

E

P

V

V

H

E

H

F

L

I

P

P

Y

Y

P

-

S

-

N

-

Y

-

R

-

P

-

P

-

F

-

G

-

V

-

G

-

G

-

E

-

E

-

G

-

H

-

R

-

I

-

A

-

S

-

R

-

Y

-

V

-

R

N

N

D

L

V

L

E

A

A

D

L

S

K

K

R

S

A

G

V

I

D

T

E

D

E

P

T

A

A

G

A

M

V

I

I

L

L

E
|
E

P

P

I

V

Q

Q

G

G

E

E

G

G

G

G

S

V

V

R

P

P

A

A

P

T

D

P

E

E

W

F

L

L

R

R

E

A

M

A

R

R

R

E

I

I

T

T

R

Q

E

E

H

K

D

G

I

A

P

L

L

L

I

I

L

L

D
|
D

E

E

I
|
I

Q
|
Q

T

T

G

G

L

M

G

G

R

R

T

T

G

G

E

K

T

R

Y

F

A

A

F

F

E

E

H

H

A

F

D

G

I

I

V

V

P

P

D

D

V

I

V

L

T

T

L

L

S

A

K
|
K

A

A

I

L

G

G

G

G

G

G

L

V

P

P

L

L

A

G

V

V

V

A

V

V

Y

M

D

R

E

E

S

E

L

V

D

A

V

R

W

S

E

M

P

P

-

K

G

G

A

G

H

H

A
x
G

G
x
T

T
|
T

F

F

R

G

G

G

N

N

Q

P

L

L

A

A

M

M

A

A

A

A

G

G

E

V

A

A

T

A

I

I

D

R

H

Y

V

L

V

E

E

R

N

T

D

R

L

L

A

W

E

E

H

R

A

A

A

A

D

E

V

L

G

G

R

P

R

W

L

F

R

M

E

E

A

K

F

L

E

R

A

A

T

I

A

P

E

S

R

-

F

-

E

P

A

K

V

I

G

R

D

E

V

V

R

R

G

G

R

M

G

G

L

L

M

M

L

V

G

G

V

L

E

E

F

L

V

K

D

E

H

K

G

A

A

A

E

P

W

Y

Q

-

G

-

P

-

G

-

P

-

H

-

A

-

P

-

D

-

G

-

D

-

F

-

A

-

E

-

A

-

V

-

Q

-

A

I

A

A

C

R

F

L

E

E

R

K

G

E

L

H

V

R

V

V

E

L

R

A

G

L

G

Q

R

A

G

G

D

P

A

T

T

V

A

I

R
|
R

F

F

L

L

P

P

P

P

L

L

I

V

V

I

T

E

R

K

A

E

Q

D

V

L

D

E

E

R

I

V

A

V

T

-

I

-

F

-

E

-

E

E

A

A

V

V

A

R

A

A

V

V

A

L

A

A

active site: S13 (≠ A22), F132 (≠ Y147), E184 (= E222), D217 (= D255), Q220 (= Q258), K246 (= K284), T275 (= T312), R363 (= R419)
binding n~2~-acetyl-n~5~-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-l-ornithine: Y16 (= Y25), Y46 (≠ A56), G105 (= G120), T106 (= T121), F132 (≠ Y147), S133 (≠ H148), R135 (≠ M150), E184 (= E222), D217 (= D255), I219 (= I257), Q220 (= Q258), K246 (= K284), G273 (≠ A310), T274 (≠ G311), T275 (= T312)

1vefA Acetylornithine aminotransferase from thermus thermophilus hb8
34% identity, 95% coverage: 15:447/454 of query aligns to 6:387/387 of 1vefA

query
sites
1vefA

Q

E

S

A

E

E

R

K

E

T

S

L

N

D

A
x
S

R

G

T

V

Y

Y

P

N

R

K

H

H

L

D

P

L

F

L

A

I

I

V

R

R

E

-

A

G

T

Q

G

G

V

A

T

R

V

V

T

W

D

D

M

A

D

E

G

G

N

N

E

E

Y

Y

Y

I

D

D

C

C

L

V

A

G

G

G

A

Y

G

G

T

V

L

A

A

N

L

L

G

G

H

H

N

G

H

N

P

P

R

E

V

V

V

V

E

E

A

A

M

V

E

K

R

R

V

Q

I

A

D

E

E

T

D

L

R

M

P

A

I

M

H

P

T

Q

L

-

D

T

I

L

S

P

T

T

P

P

T

M

K

R

E

G

R

E

F

F

V

Y

D

R

S

T

L

L

F

T

E

A

S

I

L

L

P

P

D

P

E

E

F

L

T

N

D

R

R

-

A

-

K

-

I

-

Q

V

F

F

C

P

S

V

P

N

A

S

G
|
G

T
|
T

D

E

A

A

V

N

E

E

A

A

A

A

L

L

K

K

L

F

V

A

K

R

T

A

A

H

T

T

G

G

N

R

R

K

S

K

V

F

L

V

G

A

F

A

Q

M

G

R

A

G

Y
x
F

H
x
S

G

G

M

R

T

T

S

M

G

G

A

S

L

L

G

S

L

V

M

T

G

W

D

E

V

P

D

K

V

Y

K

R

G

E

T

P

L

F

P

L

A

P

S

L

T

V

G

E

E

P

V

V

H

E

H

F

L

I

P

P

Y

Y

P

-

S

-

N

-

Y

-

R

-

P

-

P

-

F

-

G

-

V

-

G

-

G

-

E

-

E

-

G

-

H

-

R

-

I

-

A

-

S

-

R

-

Y

-

V

-

R

N

N

D

L

V

L

E

A

A

D

L

S

K

K

R

S

A

G

V

I

D

T

E

D

E

P

T

A

A

G

A

M

V

I

I

L

L

E
|
E

P

P

I

V

Q

Q

G

G

E

E

G

G

G

G

S

V

V

R

P

P

A

A

P

T

D

P

E

E

W

F

L

L

R

R

E

A

M

A

R

R

R

E

I

I

T

T

R

Q

E

E

H

K

D

G

I

A

P

L

L

L

I

I

L

L

D
|
D

E

E

I
|
I

Q

Q

T

T

G

G

L

M

G

G

R

R

T

T

G

G

E

K

T

R

Y

F

A

A

F

F

E

E

H

H

A

F

D

G

I

I

V

V

P

P

D

D

V

I

V

L

T

T

L

L

S

A

K
|
K

A

A

I

L

G

G

G

G

G

G

L

V

P

P

L

L

A

G

V

V

V

A

V

V

Y

M

D

R

E

E

S

E

L

V

D

A

V

R

W

S

E

M

P

P

-

K

G

G

A

G

H

H

A

G

G

T

T
|
T

F

F

R

G

G

G

N

N

Q

P

L

L

A

A

M

M

A

A

A

A

G

G

E

V

A

A

T

A

I

I

D

R

H

Y

V

L

V

E

E

R

N

T

D

R

L

L

A

W

E

E

H

R

A

A

A

A

D

E

V

L

G

G

R

P

R

W

L

F

R

M

E

E

A

K

F

L

E

R

A

A

T

I

A

P

E

S

R

-

F

-

E

P

A

K

V

I

G

R

D

E

V

V

R

R

G

G

R

M

G

G

L

L

M

M

L

V

G

G

V

L

E

E

F

L

V

K

D

E

H

K

G

A

A

A

E

P

W

Y

Q

-

G

-

P

-

G

-

P

-

H

-

A

-

P

-

D

-

G

-

D

-

F

-

A

-

E

-

A

-

V

-

Q

-

A

I

A

A

C

R

F

L

E

E

R

K

G

E

L

H

V

R

V

V

E

L

R

A

G

L

G

Q

R

A

G

G

D

P

A

T

T

V

A

I

R
|
R

F

F

L

L

P

P

P

P

L

L

I

V

V

I

T

E

R

K

A

E

Q

D

V

L

D

E

E

R

I

V

A

V

T

-

I

-

F

-

E

-

E

E

A

A

V

V

A

R

A

A

V

V

A

L

A

A

active site: S13 (≠ A22), F132 (≠ Y147), D217 (= D255), K246 (= K284), T275 (= T312), R363 (= R419)
binding pyridoxal-5'-phosphate: G105 (= G120), T106 (= T121), F132 (≠ Y147), S133 (≠ H148), E184 (= E222), D217 (= D255), I219 (= I257), K246 (= K284)

Q5SHH5 [LysW]-aminoadipate semialdehyde transaminase; EC 2.6.1.118 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
34% identity, 95% coverage: 15:447/454 of query aligns to 14:395/395 of Q5SHH5

query
sites
Q5SHH5

Q

E

S

A

E

E

R

K

E

T

S

L

N

D

A

S

R

G

T

V

Y

Y

P

N

R

K

H

H

L

D

P

L

F

L

A

I

I

V

R

R

E

-

A

G

T

Q

G

G

V

A

T

R

V

V

T

W

D

D

M

A

D

E

G

G

N

N

E

E

Y

Y

Y

I

D

D

C

C

L

V

A

G

G

G

A

Y

G

G

T

V

L

A

A

N

L

L

G

G

H

H

N

G

H

N

P

P

R

E

V

V

V

V

E

E

A

A

M

V

E

K

R

R

V

Q

I

A

D

E

E

T

D

L

R

M

P

A

I

M

H

P

T

Q

L

-

D

T

I

L

S

P

T

T

P

P

T

M

K

R

E

G

R

E

F

F

V

Y

D

R

S

T

L

L

F

T

E

A

S

I

L

L

P

P

D

P

E

E

F

L

T

N

D

R

R

-

A

-

K

-

I

-

Q

V

F

F

C

P

S

V

P

N

A

S

G
|
G

T
|
T

D

E

A

A

V

N

E

E

A

A

A

A

L

L

K

K

L

F

V

A

K

R

T

A

A

H

T

T

G

G

N

R

R

K

S

K

V

F

L

V

G

A

F

A

Q

M

G

R

A

G

Y

F

H

S

G

G

M

R

T

T

S

M

G

G

A

S

L

L

G

S

L

V

M

T

G

W

D

E

V

P

D

K

V

Y

K

R

G

E

T

P

L

F

P

L

A

P

S

L

T

V

G

E

E

P

V

V

H

E

H

F

L

I

P

P

Y

Y

P

-

S

-

N

-

Y

-

R

-

P

-

P

-

F

-

G

-

V

-

G

-

G

-

E

-

E

-

G

-

H

-

R

-

I

-

A

-

S

-

R

-

Y

-

V

-

R

N

N

D

L

V

L

E

A

A

D

L

S

K

K

R

S

A

G

V

I

D

T

E

D

E

P

T

A

A

G

A

M

V

I

I

L

L

E

E

P

P

I

V

Q

Q

G

G

E

E

G

G

G

G

S

V

V

R

P

P

A

A

P

T

D

P

E

E

W

F

L

L

R

R

E

A

M

A

R

R

R

E

I

I

T

T

R

Q

E

E

H

K

D

G

I

A

P

L

L

L

I

I

L

L

D

D

E

E

I

I

Q

Q

T

T

G

G

L

M

G

G

R

R

T

T

G

G

E

K

T

R

Y

F

A

A

F

F

E

E

H

H

A

F

D

G

I

I

V

V

P

P

D

D

V

I

V

L

T

T

L

L

S

A

K
|
K

A

A

I

L

G

G

G

G

G

G

L

V

P

P

L

L

A

G

V

A

V

A

V

V

Y

M

D

R

E

E

S

E

L

V

D

A

V

R

W

S

E

M

P

P

-

K

G

G

A

G

H

H

A

G

G

T

T
|
T

F

F

R

G

G

G

N

N

Q

P

L

L

A

A

M

M

A

A

A

A

G

G

E

V

A

A

T

A

I

I

D

R

H

Y

V

L

V

E

E

R

N

T

D

R

L

L

A

W

E

E

H

R

A

A

A

A

D

E

V

L

G

G

R

P

R

W

L

F

R

M

E

E

A

K

F

L

E

R

A

A

T

I

A

P

E

S

R

-

F

-

E

P

A

K

V

I

G

R

D

E

V

V

R

R

G

G

R

M

G

G

L

L

M

M

L

V

G

G

V

L

E

E

F

L

V

K

D

E

H

K

G

A

A

A

E

P

W

Y

Q

-

G

-

P

-

G

-

P

-

H

-

A

-

P

-

D

-

G

-

D

-

F

-

A

-

E

-

A

-

V

-

Q

-

A

I

A

A

C

R

F

L

E

E

R

K

G

E

L

H

V

R

V

V

E

L

R

A

G

L

G

Q

R

A

G

G

D

P

A

T

T

V

A

I

R

R

F

F

L

L

P

P

P

P

L

L

I

V

V

I

T

E

R

K

A

E

Q

D

V

L

D

E

E

R

I

V

A

V

T

-

I

-

F

-

E

-

E

E

A

A

V

V

A

R

A

A

V

V

A

L

A

A

GT 113:114 (= GT 120:121) binding pyridoxal 5'-phosphate
K254 (= K284) modified: N6-(pyridoxal phosphate)lysine
T283 (= T312) binding pyridoxal 5'-phosphate

O58478 Alanine/serine racemase; ASR; Ala/Ser racemase; EC 5.1.1.-; EC 5.1.1.1 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see paper)
32% identity, 92% coverage: 27:442/454 of query aligns to 50:458/474 of O58478

query
sites
O58478

R

K

H

L

L

F

P

P

F

L

A

V

I

P

R

K

E

R

A

G

T

F

G

G

V

P

T

F

V

I

T

E

D

D

M

V

D

D

G

G

N

N

E

V

Y

F

Y

I

D

D

C

F

L

L

A

A

G

G

A

A

G

A

T

A

L

A

A

S

L

T

G

G

H

Y

N

A

H

H

P

P

R

K

V

L

V

V

E

K

A

A

M

V

E

K

R

E

V

Q

I

V

D

E

E

L

D

I

R

Q

P

-

I

-

H

H

T

S

L

M

D

I

I

G

S

Y

T

T

P

H

T

S

K

E

E

R

-

A

-

I

R

R

F

V

V

A

D

E

S

K

L

L

F

V

E

E

S

I

L

S

P

P

D

I

E

E

F

-

T

-

D

-

R

N

A

S

K

K

I

V

Q

I

F

F

C

G

S

L

P

-

A

S

G

G

T

S

D

D

A

A

V

V

E

D

A

M

A

A

L

I

K

K

L

V

V

S

K

K

T

F

A

S

T

T

G

R

N

R

R

P

S

W

V

I

L

L

G

A

F

F

Q

I

G

G

A

A

Y

Y

H

H

G

G

M

Q

T

T

S

L

G

G

A

A

L

T

G

S

L

V

M

A

G

S

-

F

D

Q

V

V

D

S

V

Q

K

K

G

R

T

G

L

Y

P

S

A

P

S

L

T

M

G

P

E

N

V

V

H

F

H

W

L

I

P

P

Y

Y

P

P

S

N

N

P

Y

F

R

R

P

N

P

I

F

W

G

G

V

I

G

N

G

G

-

Y

E

E

E

E

G

P

H

D

R

E

I

L

A

I

S

N

R

R

Y

V

V

L

R

D

N

Y

L

L

L

E

A

Y

D

Y

S

V

K

F

S

S

G

H

I

V

T

V

D

P

P

P

-

D

-

E

-

V

A

A

G

A

M

L

I

F

L

A

E

E

P

P

I

I

Q

Q

G

G

E
x
D

G

A

G

G

S

I

V

V

P

V

A

P

P

P

D

E

E

N

W

F

L

F

R

K

E

E

M

L

R

K

R

K

I

L

T

L

R

E

E

E

H

Y

D

G

I

I

P

L

L

L

I

V

L

M

D

D

E

E

I

V

Q

Q

T

T

G

G

L

I

G

G

R

R

T

T

G

G

E

K

T

W

Y

F

A

A

F

S

E

E

H

W

A

F

D

N

I

V

V

K

P

P

D

D

V

M

V

I

T

I

L

F

S

G

K
|
K

A

G

I

V

G

A

G

S

G

G

L

M

P

G

L

L

A

S

-

G

V

V

V

I

V

G

Y

R

D

K

E

E

S

I

L

M

D

D

V

I

W

-

E

T

P

S

G

G

A

S

H

A

A

L

G

L

T

T

F

P

R

A

G

A

N

N

Q

P

L

V

A

I

M

S

A

A

A

A

G

A

E

E

A

A

T

T

I

L

D

E

H

I

V

I

V

E

E

E

N

E

D

N

L

L

A

L

E

K

H

N

A

A

A

L

D

E

V

V

G

G

R

E

R

F

L

I

R

M

E

G

A

R

F

L

E

K

A

E

T

I

A

K

E

E

R

R

F

F

E

E

A

I

V

I

G

G

D

D

V

V

R

R

G

G

R

K

G

G

L

L

M

M

L

I

G

G

V

V

E

E

F

I

V

V

D

K

H

E

G

N

A

G

E

R

W

-

Q

-

G

-

P

-

G

-

P

-

H

-

A

-

P

P

D

D

G

P

D

E

F

M

A

T

E

G

A

K

V

I

Q

C

A

W

A

R

C

A

F

F

E

E

R

L

G

G

L

L

V

I

V

L

E

P

R

S

G

Y

G

G

R

M

G

F

D

G

A

N

T

V

A

I

R

R

F

I

L

T

P

P

P

P

L

L

I

V

V

L

T

T

R

K

A

E

Q

V

V

A

D

E

E

K

I

A

A

L

T

E

I

I

F

I

E

E

E

R

A

A

V

I

D251 (≠ E227) mutation to A: Loss of activity.
K308 (= K284) mutation to A: Loss of activity.

2eo5A Crystal structure of 4-aminobutyrate aminotransferase from sulfolobus tokodaii strain7
31% identity, 93% coverage: 26:445/454 of query aligns to 21:412/412 of 2eo5A

query
sites
2eo5A

P

P

R

E

H

L

L

F

P

P

F

L

A

V

I

I

R

D

E

H

A

G

T

E

G

G

V

V

T

W

V

I

T

Y

D

D

M

V

D

D

G

G

N

N

E

K

Y

Y

Y

L

D

D

C

F

L

T

A

S

G

G

A

I

G

G

T

V

L

N

A

N

L

L

G

G

H

W

-

P

N

S

H

H

P

P

R

E

V

V

V

I

E

K

A

I

M

G

E

I

R

E

V

Q

I

M

D

Q

E

K

D

L

R

A

P

H

I

A

H

A

T

A

L

N

D

D

I

F

S

Y

T

N

P

I

T

P

K

Q

E

L

R

E

F

L

V

A

D

K

S

K

L

L

F

V

E

T

S

Y

L

S

P

P

D

G

E

N

F

F

T

Q

D

K

R

K

A

V

K

-

I

-

Q

-

F

F

C

F

S

S

P

N

A

S

G
|
G

T
|
T

D

E

A

A

V

I

E

E

A

A

A

S

L

I

K

K

L

V

V

V

K

K

T

N

A

-

T

T

G

G

N

R

R

K

S

Y

V

I

L

I

G

A

F

F

Q

L

G

G

A

G

Y
x
F

H
|
H

G

G

M

R

T

T

S

F

G

G

A

S

L

I

G

S

L

L

M

T

G

A

D

S

V

K

D

A

V

V

K

Q

G

R

T

S

L

I

-

V

-

G

P

P

A

F

S

M

T

P

G

G

E

-

V

V

H

I

H

H

L

V

P

P

Y

Y

P

P

S

N

N

P

Y

Y

R

R

P

N

P

P

F

W

G

H

V

I

G

N

G

G

E

Y

E

E

G

N

-

P

H

S

R

E

I

L

A

V

S

N

R

R

Y

V

V

I

R

E

N

F

L

I

L

E

A

D

D

Y

S

I

K

F

S

V

G

N

I

L

T

V

D

P

P

P

-

E

-

E

-

V

A

A

G

G

M

I

I

F

L

F

E
|
E

P

P

I

I

Q

Q

G

G

E

E

G

G

G

G

S

Y

V

V

P

I

A

P

P

P

D

K

E

N

W

F

L

F

R

A

E

E

M

L

R

Q

R

K

I

L

T

A

R

K

E

K

H

Y

D

G

I

I

P

L

L

L

I

V

L

D

D
|
D

E

E

I
x
V

Q
|
Q

T

M

G

G

L

L

G

G

R

R

T

T

G

G

E

K

T

L

Y

F

A

A

F

I

E

E

H

N

A

F

D

N

I

T

V

V

P

P

D

D

V

V

V

I

T

T

L

L

S

A

K
|
K

A

A

I

L

G

G

G

G