SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_007694754.1 NCBI__GCF_000336675.1:WP_007694754.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4zrnA Crystal structure of udp-glucose 4-epimerase (tm0509) with udp-glucose from hyperthermophilic eubacterium thermotoga maritima (see paper)
33% identity, 97% coverage: 6:310/316 of query aligns to 3:309/309 of 4zrnA

query
sites
4zrnA

V

I

L

L

V

V

T

T

G
|
G

G

G

A

A

G
|
G

F
|
F

I
|
I

G

G

S

S

N

H

L

V

A

V

N

D

H

K

L

L

A

I

E

E

S

N

N

G

-

Y

D

G

V

V

I

I

A

V

L

V

D
|
D

N
|
N

G

L

Y
x
S

L
x
S

G
|
G

T

K

E

V

E

E

N

N

L

L

N

N

N

R

K

N

V

A

E

L

F

F

V

Y

E

E

A

Q

S
|
S

V
x
I

L

E

D

D

D

E

D

E

L

M

P

M

T

E

N

R

V

I

-

F

-

S

-

L

-

H

-

R

-

P

D

E

V

Y

V

V

F

F

H

H

L
|
L

A
|
A

A
|
A

L

Q

S

A

S

S

Y

V

A

A

M

I

H

S

E

V

A

R

N

E

P

P

Q

A

Q

R

G

D

A

A

R

K

V
x
T

N

N

V

I

K

I

G

G

F

S

V

L

N

V

V

L

V

L

E

E

Q

K

A

S

R

I

N

K

Q

Y

G

G

C

V

E

K

T

K

V

F

V

I

Y

F

A
x
S

S
|
S

T
|
T

S

G

-
x
G

S
x
A

I

I

Y

Y

G

G

N

E

R

N

T

V

E

K

-

V

-

F

P

P

S

T

P

P

E

E

D

T

M

E

D

I

V

P

Q

H

A

P

N

I

T

S

G

P

Y
|
Y

E

G

A

I

S

A

K
|
K

L

Y

A

S

R

T

E

E

R

M

Y

Y

A

L

E

E

Y

F

Y

F

S

A

N

R

F

E

H

Y

D

G

M

L

T

K

C

Y

A

T

G

V

M

L

R

R

F
x
Y

F

A

S
x
N

V
|
V

F

-

Q

-

G

-

Y

Y

G

G

G

P

S

R

E

Q

A

D

H

P

K
x
Y

G

G

E

E

Y

-

A

A

N
x
G

V
|
V

I

V

A

A

Q

I

F

F

A

T

D

E

S

R

L

M

A

L

T

R

G

G

E

E

T

E

P

V

V
x
H

L

I

Y
x
F

G

G

D

D

G

G

T

E

Q
x
Y

T

V

R
|
R

D

D

F

Y

T

V

H

Y

V

V

Q

D

D

D

I

V

V

V

R

R

G

A

L

N

E

L

I

L

A

A

A

M

D

E

E

K

R

G

L

D

D

N

G

E

I

V

Y

F

N

N

L

I

G

G

T

T

G

G

E

R

S

G

Y

T

S

T

F
x
V

N

N

T

Q

I

L

I

F

E

K

L

L

L

L

N

K

D

E

E

I

L

T

G

G

T

Y

D

D

I

K

E

E

P

P

D

-

H

-

V

V

E

Y

N

K

P

P

I

-

P

P

E
x
R

D

K

V

G

Y
x
D

V

V

H

R

D

K

T

S

M

I

A

L

D

D

I

Y

T

T

K

K

M

A

T

K

T

E

E

K

T

L

S

G

W

W

E

E

P

P

Q

K

I

V

T

S

F

L

E

E

E

E

G

G

I

L

R

K

L

L

V

-

T

T

N

V

E

E

Y

Y

R

F

N

R

E

K

S

T

L

L

E

E

active site: T117 (= T113), G119 (vs. gap), A120 (≠ S115), Y143 (= Y136), K147 (= K140), Y181 (≠ K177), G185 (≠ N182)
binding nicotinamide-adenine-dinucleotide: G7 (= G10), G10 (= G13), F11 (= F14), I12 (= I15), D31 (= D33), N32 (= N34), S34 (≠ Y36), S35 (≠ L37), G36 (= G38), S51 (= S53), I52 (≠ V54), L73 (= L69), A74 (= A70), A75 (= A71), T92 (≠ V88), S115 (≠ A111), S116 (= S112), Y143 (= Y136), K147 (= K140), Y170 (≠ F163), V173 (= V166)
binding uridine-5'-diphosphate-glucose: T117 (= T113), G119 (vs. gap), A120 (≠ S115), Y143 (= Y136), N172 (≠ S165), G185 (≠ N182), V186 (= V183), H201 (≠ V198), F203 (≠ Y200), Y208 (≠ Q205), R210 (= R207), V244 (≠ F241), R267 (≠ E267), D270 (≠ Y270)

6dntA Udp-n-acetylglucosamine 4-epimerase from methanobrevibacter ruminantium m1 in complex with udp-n-acetylmuramic acid (see paper)
32% identity, 94% coverage: 1:298/316 of query aligns to 1:300/310 of 6dntA

query
sites
6dntA

M

M

N

K

G

D

K

K

R

N

V

V

L

V

V

V

T

T

G
|
G

G

G

A

L

G
|
G

F
|
F

I
|
I

G

G

S

S

N

H

L

I

A

V

N

D

H

A

L

L

A

I

E

D

S

D

N

N

D

K

V

V

I

T

A

I

L

I

D
|
D

N
|
N

G
x
L

Y
x
S

L
x
S

G
|
G

T

K

E

M

E

E

N

N

L

L

N

N

N

N

K

P

-

N

-

H

-

E

-

N

V

L

E

T

F

I

V

I

E

K

A

E

S
x
D

V
x
L

L

M

D

D

D

A

D

D

L

L

P

E

T

K

-

I

-

L

-

K

N

D

V

K

D

D

V

Y

V

V

F

F

H

H

L
|
L

A
|
A

A
|
A

L

L

S
x
A

S

S

Y

V

A

P

M

G

H

S

E

V

A

A

N

E

P

P

Q

L

Q

R

G

Y

A

N

R

Q

V

N

N

N

V

I

K

D

G

A

F

S

V

L

N

K

V

L

V

F

E

I

Q

A

A

C

R

K

N

N

Q

N

G

N

C

I

E

K

T

K

V

V

V

I

Y

F

A
x
S

S
|
S

T
x
S

S
|
S

S
x
A

I

V

Y

Y

G

G

-

E

N

N

R

P

T

N

E

M

P

P

S

L

P

K

E

E

D

S

M

E

D

N

V

F

Q

L

A

P

N

C

T

S

G

P

Y
|
Y

E

A

A

A

S

Q

K
|
K

L

A

A

S

R

C

E

E

R

L

Y

Y

A

L

E

K

Y

S

Y

F

S

H

N

E

F

S

H

Y

D

G

M

L

T

D

C

Y

A

V

G

A

M

L

R

R

F
x
Y

F
|
F

S
x
N

V
|
V

F

F

Q

-

G

-

Y

-

G

G

G

P

S

R

E

Q

A

D

H

E

K

N

G

S

E

P

Y

Y

A

A

N
x
A

V
|
V

I

I

A

P

Q
x
K

F

F

A

I

D

S

S

A

L

I

A

L

T

N

G

G

E

E

T

S

P

P

V
|
V

L
x
I

Y
|
Y

G

G

D

D

G

G

T
x
E

Q
|
Q

T

S

R
|
R

D

D

F

F

T

I

H

Y

V

V

Q

K

D

E

I

I

V

A

R

K

G

A

L

N

E

I

I

L

A

S

A

A

D

E

E

S

R

D

L

Y

D

N

G

G

I

V

Y

I

N

N

L

V

G

A

T

L

G

G

E

K

S

S

Y

M

S

T

F
x
I

N

N

T

R

I

L

I

F

E

E

L

I

L

I

N

S

D

D

E

V

L

L

G

E

T

S

D

D

I

I

E

D

P

V

D

K

H

Y

V

L

E

D

N

E

P
x
R

I

-

P

P

E

G

D
|
D

V

-

Y

-

V

I

H

K

D
x
H

T

S

M

L

A

A

D

D

I

I

T

S

K

N

M

L

T

-

T

D

E

K

T

I

S

S

W

F

E

K

P

P

-

D

Q

E

I

D

T

K

F

F

E

E

E

E

G

Q

I

L

R

R

active site: S120 (≠ T113), S121 (= S114), A122 (≠ S115), Y144 (= Y136), K148 (= K140), A187 (≠ N182)
binding (2R)-2-{[(2R,3R,4R,5S,6R)-3-(acetylamino)-2-{[(S)-{[(R)-{[(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methoxy}(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]oxy}-5-hydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-4-yl]oxy}propanoic acid: A80 (≠ S73), S120 (≠ T113), S121 (= S114), Y144 (= Y136), F172 (= F164), N173 (≠ S165), A187 (≠ N182), V188 (= V183), K191 (≠ Q186), V203 (= V198), I204 (≠ L199), Y205 (= Y200), Q210 (= Q205), R212 (= R207), I246 (≠ F241), R269 (≠ P264), D272 (= D268)
binding nicotinamide-adenine-dinucleotide: G10 (= G10), G13 (= G13), F14 (= F14), I15 (= I15), D33 (= D33), N34 (= N34), L35 (≠ G35), S36 (≠ Y36), S37 (≠ L37), G38 (= G38), D57 (≠ S53), L58 (≠ V54), L76 (= L69), A77 (= A70), A78 (= A71), A80 (≠ S73), S118 (≠ A111), S119 (= S112), Y144 (= Y136), K148 (= K140), Y171 (≠ F163), V174 (= V166)
binding zinc ion: E209 (≠ T204), H275 (≠ D273)

6pmhA Structure of epimerase mth375 from the thermophilic pseudomurein- containing methanogen methanothermobacter thermautotrophicus
35% identity, 94% coverage: 2:297/316 of query aligns to 8:310/330 of 6pmhA

query
sites
6pmhA

N

D

G

G

K

K

R

C

V

V

L

L

V

V

T

T

G
|
G

G

G

A

A

G
|
G

F

C

I

V

G

G

S

S

N

N

L

L

A

T

N

G

H

N

L

L

A

A

E

K

S

A

N

G

-

A

D

N

V

V

I

I

A

I

L

L

D
|
D

N
|
N

G

L

Y
x
S

L
x
S

G
x
S

T

Y

E

E

E

W

N

N

L

I

N

P

N

E

-

Y

-

E

K

N

V

I

E

E

F

F

V

V

E

K

A

G

S
x
D

V
x
I

L

L

D

D

D

D

D

E

L

V

P

L

T

K

N

R

V

V

-

F

-

K

-

E

-

R

-

P

D

D

V

Y

V

V

F

F

H

H

L

L

A
|
A

A

A

L

H

S
x
F

S

A

Y

N

A

Q

M

N

H

S

E

V

A

D

N

N

P

P

Q

E

Q

K

G

D

A

L

R

L

V

V

N

N

V

G

K

L

G

G

F

I

V

L

N

K

V

V

V

L

E

E

Q

Y

A

A

R

Q

N

L

Q

V

G

G

C

V

E

E

T

R

V

F

V

V

Y

Y

A

S

S

S

T
x
S

S

G

S
x
C

-
x
G

I

V

Y

Y

G

G

-

L

-

D

N

S

R

K

T

I

E

P

P

F

S

E

P

E

E

H

D

D

M

I

D

S

V

I

Q

S

A

L

N

H

T

T

G

P

Y
|
Y

E

Q

A

V

S

T

K
|
K

L

L

A

L

R

G

E

E

R

L

Y

Y

A

T

E

N

Y

Y

Y

F

S

H

N

N

F

L

H

Y

D

E

M

M

T

P

C

I

A

V

G

N

M

A

R

R

F

F

F

F

S
x
N

V

V

F

F

Q

-

G

-

Y

-

G

-

G

G

S

P

E

G

A

E

H

V

K

P

G

G

E

K

Y

Y

A
x
R

N
|
N

V
|
V

I

I

A

P

Q

N

F
|
F

A

F

D

-

S

Y

L

W

A

A

T

M

G

N

E

Q

T

Q

P

P

V

L

-
x
P

L
x
I

Y
x
T

G

G

D

D

G

G

T

S

Q

E

T

T

R
|
R

D

D

F

W

T

T

H

F

V

V

Q

E

D

D

I

I

V

V

R

R

G

G

L

L

E

-

I

M

A

A

A

M

D

G

E

V

R

R

L

R

D

E

G

A

I

I

-

G

-

E

-

A

Y

I

N

N

L

L

G

G

T

S

G

G

E

T

S

E

Y

H

S

Q

F
x
V

N

I

T

E

I

M

I

A

E

G

L

I

L

I

N

N

D

E

E

L

L

-

G

-

T

T

D

E

I

N

E

P

P

A

D

G

H

V

V

V

E

Y

N

R

P

P

I

R

P
x
R

E

D

D

W

V

D

Y

A

V

K

H

T

D

R

T

L

M

L

A

S

D

S

I

I

T

D

K

K

M

A

T

R

T

R

E

L

T

L

S

D

W

Y

E

E

P

P

Q

Q

I

V

T

S

F

F

E

R

E

E

G

G

I

L

active site: S127 (≠ T113), C129 (≠ S115), G130 (vs. gap), Y153 (= Y136), K157 (= K140)
binding adenosine monophosphate: G16 (= G10), G19 (= G13), D40 (= D33), N41 (= N34), S43 (≠ Y36), S44 (≠ L37), S45 (≠ G38), D62 (≠ S53), I63 (≠ V54), A84 (= A70), F87 (≠ S73), R194 (≠ A181)
binding uridine-5'-diphosphate: C129 (≠ S115), N182 (≠ S165), N195 (= N182), V196 (= V183), F200 (= F187), P211 (vs. gap), I212 (≠ L199), T213 (≠ Y200), R220 (= R207), V256 (≠ F241), R279 (≠ P266)

6pnlA Structure of epimerase mth375 from the thermophilic pseudomurein- containing methanogen methanothermobacter thermautotrophicus
35% identity, 94% coverage: 2:297/316 of query aligns to 14:316/336 of 6pnlA

query
sites
6pnlA

N

D

G

G

K

K

R

C

V

V

L

L

V

V

T

T

G
|
G

G

G

A

A

G
|
G

F
x
C

I

V

G

G

S

S

N

N

L

L

A

T

N

G

H

N

L

L

A

A

E

K

S

A

N

G

-

A

D

N

V

V

I

I

A

I

L

L

D
|
D

N
|
N

G
x
L

Y
x
S

L
x
S

G
x
S

T

Y

E

E

E

W

N

N

L

I

N

P

N

E

-

Y

-

E

K

N

V

I

E

E

F

F

V

V

E

K

A

G

S
x
D

V
x
I

L

L

D

D

D

D

D

E

L

V

P

L

T

K

N

R

V

V

-

F

-

K

-

E

-

R

-

P

D

D

V

Y

V

V

F

F

H

H

L
|
L

A

A

A
|
A

L

H

S
x
F

S

A

Y

N

A

Q

M

N

H

S

E

V

A

D

N

N

P

P

Q

E

Q

K

G

D

A

L

R

L

V
|
V

N

N

V

G

K

L

G

G

F

I

V

L

N

K

V

V

V

L

E

E

Q

Y

A

A

R

Q

N

L

Q

V

G

G

C

V

E

E

T

R

V

F

V

V

Y

Y

A
x
S

S

S

T
x
S

S

G

S
x
C

-
x
G

I

V

Y

Y

G

G

-

L

-

D

N

S

R

K

T

I

E

P

P

F

S

E

P

E

E

H

D

D

M

I

D

S

V

I

Q

S

A

L

N

H

T

T

G

P

Y
|
Y

E

Q

A

V

S

T

K
|
K

L

L

A

L

R

G

E

E

R

L

Y

Y

A

T

E

N

Y

Y

Y

F

S

H

N

N

F

L

H

Y

D

E

M

M

T

P

C

I

A

V

G

N

M

A

R

R

F
|
F

F

F

S
x
N

V
|
V

F

F

Q

-

G

-

Y

-

G

-

G

G

S

P

E

G

A

E

H

V

K

P

G

G

E

K

Y

Y

A
x
R

N
|
N

V
|
V

I

I

A

P

Q

N

F
|
F

A

F

D

-

S

Y

L

W

A

A

T

M

G

N

E

Q

T

Q

P

P

V

L

-
x
P

L
x
I

Y
x
T

G

G

D

D

G

G

T

S

Q

E

T

T

R
|
R

D

D

F

W

T

T

H

F

V

V

Q

E

D

D

I

I

V

V

R

R

G

G

L

L

E

-

I

M

A

A

A

M

D

G

E

V

R

R

L

R

D

E

G

A

I

I

-

G

-

E

-

A

Y

I

N

N

L

L

G

G

T

S

G

G

E

T

S

E

Y

H

S

Q

F
x
V

N

I

T

E

I

M

I

A

E

G

L

I

L

I

N

N

D

E

E

L

L

-

G

-

T

T

D

E

I

N

E

P

P

A

D

G

H

V

V

V

E

Y

N

R

P

P

I

R

P
x
R

E

D

D

W

V

D

Y

A

V

K

H

T

D

R

T

L

M

L

A

S

D

S

I

I

T

D

K

K

M

A

T

R

T

R

E

L

T

L

S

D

W

Y

E

E

P

P

Q

Q

I

V

T

S

F

F

E

R

E

E

G

G

I

L

active site: S133 (≠ T113), C135 (≠ S115), G136 (vs. gap), Y159 (= Y136), K163 (= K140)
binding nicotinamide-adenine-dinucleotide: G22 (= G10), G25 (= G13), C26 (≠ F14), D46 (= D33), N47 (= N34), L48 (≠ G35), S49 (≠ Y36), S50 (≠ L37), S51 (≠ G38), D68 (≠ S53), I69 (≠ V54), L89 (= L69), A91 (= A71), F93 (≠ S73), V108 (= V88), S131 (≠ A111), S133 (≠ T113), Y159 (= Y136), K163 (= K140), F186 (= F163), N188 (≠ S165), V189 (= V166), R200 (≠ A181)
binding uridine-5'-diphosphate: N188 (≠ S165), N201 (= N182), V202 (= V183), F206 (= F187), P217 (vs. gap), I218 (≠ L199), T219 (≠ Y200), R226 (= R207), V262 (≠ F241), R285 (≠ P266)

1sb8A Crystal structure of pseudomonas aeruginosa udp-n-acetylglucosamine 4- epimerase complexed with udp-n-acetylgalactosamine (see paper)
31% identity, 95% coverage: 4:304/316 of query aligns to 17:336/341 of 1sb8A

query
sites
1sb8A

K

K

R

V

V

W

L

L

V

I

T

T

G
|
G

G

V

A

A

G
|
G

F
|
F

I
|
I

G

G

S

S

N

N

L

L

A

L

N

E

H

T

L

L

A

L

E

K

-

L

S

D

N

Q

D

K

V

V

I

V

A

G

L

L

D
|
D

N
|
N

G
x
F

Y

A

L
x
T

G
|
G

T

H

E

Q

E

R

N

N

L

L

N

D

-

E

-

V

-

R

-

S

-

L

-

V

-

S

-

E

-

K

-

Q

-

W

N

S

K

N

V

F

E

K

F

F

V

I

E

Q

A

G

S
x
D

V
x
I

-

R

-

N

L

L

D

D

D

D

-

C

-

N

D

N

L

A

P

C

T

A

N

G

V

V

D

D

V

Y

V

V

F

L

H

H

L
x
Q

A

A

A
|
A

L

L

S

G

S
|
S

Y

V

A

P

M

R

H

S

E

I

A

N

N

D

P

P

Q

I

Q

T

G

S

A

N

R

A

V
x
T

N

N

V

I

K

D

G

G

F

F

V

L

N

N

V

M

V

L

E

I

Q

A

A

A

R

R

N

D

Q

A

G

K

C

V

E

Q

T

S

V

F

V

T

Y

Y

A
|
A

S
x
A

T
x
S

S
|
S

S
|
S

I

T

Y

Y

G

G

N

D

R

H

T

P

E

G

-

L

P

P

S

K

P

V

E

E

D

D

M

T

D

I

V

G

Q

K

A

P

N

L

T

S

G

P

Y
|
Y

E

A

A

V

S

T

K
|
K

L

Y

A

V

R

N

E

E

R

L

Y

Y

A

A

E

D

Y

V

Y

F

S

S

N

R

F

C

H

Y

D

G

M

F

T

S

C

T

A

I

G

G

M

L

R

R

F
x
Y

F

F

S
x
N

V
|
V

F

F

Q

-

G

-

Y

-

G

G

G

R

S

R

E

Q

A

D

H

P

K
x
N

G

G

E

A

Y

Y

A

A

N
x
A

V
|
V

I

I

A

P

Q

K

F
x
W

A

T

D

S

S

S

L

M

A

I

T

Q

G

G

E

D

T

D

P

V

V
x
Y

L
x
I

Y
x
N

G

G

D

D

G

G

T

E

Q

T

T

S

R
|
R

D

D

F

F

T

C

H

Y

V

I

Q

E

D

N

I

T

V

V

R

Q

G

A

L

N

E

L

I

L

A

A

A

A

D

T

E

A

R

G

L

L

D

D

G

A

-

R

-

N

-

Q

I

V

Y

Y

N

N

L

I

G

A

T

V

G

G

E

G

S

R

Y

T

S

S

F
x
L

N

N

T

Q

I

L

I

F

E

F

L

A

L

L

N

R

D

D

E

G

L

L

G

A

T

E

D

N

I

G

E

V

P

S

D

Y

H

H

V

R

E

E

N

-

P

P

I

V

P

Y

E

R

D

D

V

F

Y
x
R

-

E

-

G

-
x
D

V

V

H

R

D

H

T
x
S

M

L

A

A

D

D

I

I

T

S

K

K

M

A

T

A

T

K

E

L

T

L

S

G

W

Y

E

A

P

P

Q

K

I

Y

T

D

F

V

E

S

E

A

G

G

I

V

R

A

L

L

V

A

T

M

N

P

E

W

Y

Y

active site: S142 (≠ T113), S143 (= S114), S144 (= S115), Y166 (= Y136), K170 (= K140), N204 (≠ K177)
binding nicotinamide-adenine-dinucleotide: G23 (= G10), G26 (= G13), F27 (= F14), I28 (= I15), D47 (= D33), N48 (= N34), F49 (≠ G35), T51 (≠ L37), G52 (= G38), D78 (≠ S53), I79 (≠ V54), Q98 (≠ L69), A100 (= A71), T117 (≠ V88), A140 (= A111), A141 (≠ S112), Y166 (= Y136), K170 (= K140), Y193 (≠ F163), N195 (≠ S165), V196 (= V166)
binding uridine-diphosphate-n-acetylgalactosamine: S103 (= S74), S142 (≠ T113), S143 (= S114), S144 (= S115), Y166 (= Y136), N195 (≠ S165), A209 (≠ N182), V210 (= V183), W214 (≠ F187), Y225 (≠ V198), I226 (≠ L199), N227 (≠ Y200), R234 (= R207), L271 (≠ F241), R299 (≠ Y270), D302 (vs. gap), S306 (≠ T274)

1sb9A Crystal structure of pseudomonas aeruginosa udp-n-acetylglucosamine 4- epimerase complexed with udp-glucose (see paper)
31% identity, 95% coverage: 4:304/316 of query aligns to 16:335/340 of 1sb9A

query
sites
1sb9A

K

K

R

V

V

W

L

L

V

I

T

T

G
|
G

G

V

A

A

G
|
G

F
|
F

I
|
I

G

G

S

S

N

N

L

L

A

L

N

E

H

T

L

L

A

L

E

K

-

L

S

D

N

Q

D

K

V

V

I

V

A

G

L

L

D
|
D

N
|
N

G
x
F

Y

A

L
x
T

G
|
G

T

H

E

Q

E

R

N

N

L

L

N

D

-

E

-

V

-

R

-

S

-

L

-

V

-

S

-

E

-

K

-

Q

-

W

N

S

K

N

V

F

E

K

F

F

V

I

E

Q

A

G

S
x
D

V
x
I

-

R

-

N

L

L

D

D

D

D

-

C

-

N

D

N

L

A

P

C

T

A

N

G

V

V

D

D

V

Y

V

V

F

L

H

H

L
x
Q

A

A

A
|
A

L

L

S

G

S

S

Y

V

A

P

M

R

H

S

E

I

A

N

N

D

P

P

Q

I

Q

T

G

S

A

N

R

A

V
x
T

N

N

V

I

K

D

G

G

F

F

V

L

N

N

V

M

V

L

E

I

Q

A

A

A

R

R

N

D

Q

A

G

K

C

V

E

Q

T

S

V

F

V

T

Y

Y

A
|
A

S
x
A

T
x
S

S
|
S

S
|
S

I

T

Y

Y

G

G

N

D

R

H

T

P

E

G

-

L

P

P

S

K

P

V

E

E

D

D

M

T

D

I

V

G

Q

K

A

P

N

L

T

S

G

P

Y
|
Y

E

A

A

V

S

T

K
|
K

L

Y

A

V

R

N

E

E

R

L

Y

Y

A

A

E

D

Y

V

Y

F

S

S

N

R

F

C

H

Y

D

G

M

F

T

S

C

T

A

I

G

G

M

L

R

R

F
x
Y

F

F

S
x
N

V
|
V

F

F

Q

-

G

-

Y

-

G

G

G

R

S

R

E

Q

A

D

H

P

K
x
N

G

G

E

A

Y

Y

A

A

N
x
A

V
|
V

I

I

A

P

Q

K

F
x
W

A

T

D

S

S

S

L

M

A

I

T

Q

G

G

E

D

T

D

P

V

V
x
Y

L
x
I

Y
x
N

G

G

D

D

G

G

T

E

Q

T

T

S

R

R

D

D

F

F

T

C

H

Y

V

I

Q

E

D

N

I

T

V

V

R

Q

G

A

L

N

E

L

I

L

A

A

A

A

D

T

E

A

R

G

L

L

D

D

G

A

-

R

-

N

-

Q

I

V

Y

Y

N

N

L

I

G

A

T

V

G

G

E

G

S

R

Y

T

S

S

F
x
L

N

N

T

Q

I

L

I

F

E

F

L

A

L

L

N

R

D

D

E

G

L

L

G

A

T

E

D

N

I

G

E

V

P

S

D

Y

H

H

V

R

E

E

N

-

P

P

I

V

P

Y

E

R

D

D

V

F

Y
x
R

-

E

-

G

-
x
D

V

V

H

R

D

H

T

S

M

L

A

A

D

D

I

I

T

S

K

K

M

A

T

A

T

K

E

L

T

L

S

G

W

Y

E

A

P

P

Q

K

I

Y

T

D

F

V

E

S

E

A

G

G

I

V

R

A

L

L

V

A

T

M

N

P

E

W

Y

Y

active site: S141 (≠ T113), S142 (= S114), S143 (= S115), Y165 (= Y136), K169 (= K140), N203 (≠ K177)
binding nicotinamide-adenine-dinucleotide: G22 (= G10), G25 (= G13), F26 (= F14), I27 (= I15), D46 (= D33), N47 (= N34), F48 (≠ G35), T50 (≠ L37), G51 (= G38), D77 (≠ S53), I78 (≠ V54), Q97 (≠ L69), A99 (= A71), T116 (≠ V88), A139 (= A111), A140 (≠ S112), Y165 (= Y136), K169 (= K140), Y192 (≠ F163), N194 (≠ S165), V195 (= V166)
binding uridine-5'-diphosphate-glucose: S141 (≠ T113), Y165 (= Y136), N194 (≠ S165), A208 (≠ N182), V209 (= V183), W213 (≠ F187), Y224 (≠ V198), I225 (≠ L199), N226 (≠ Y200), L270 (≠ F241), R298 (≠ Y270), D301 (vs. gap)

7ystA Crystal structure of udp-glucose 4-epimerase (rv3634c) in complex with both udp-glucose and udp-galactose in chain b from mycobacterium tuberculosis
34% identity, 96% coverage: 5:307/316 of query aligns to 2:311/312 of 7ystA

query
sites
7ystA

R

R

V

A

L

L

V

V

T

T

G
|
G

G

A

A

A

G
|
G

F
|
F

I
|
I

G

G

S

S

N

T

L

L

A

V

N

D

H

R

L

L

-

L

A

A

E

D

S

G

N

H

D

S

V

V

I

V

A

G

L

L

D
|
D

N
|
N

G

F

Y

A

L
x
T

G
|
G

T

R

E

A

E

T

N

N

L

L

-

E

-

H

-

L

-

A

-

D

N

N

N

S

K

A

V

H

E

V

F

F

V

V

E

E

A

A

S
x
D

V
x
I

L

V

D

T

D

A

D

D

L

L

P

H

T

A

-

I

-

L

-

E

-

Q

-

H

N

R

V

P

D

E

V

V

V

V

F

F

H

H

L
|
L

A
|
A

A
|
A

L

Q

S
x
I

S

D

Y

V

A
x
R

M

R

H

S

E

V

A

A

N

D

P

P

Q

Q

Q

F

G

D

A

A

R

A

V
|
V

N

N

V

V

K

I

G

G

F

T

V

V

N

R

V

L

V

A

E

E

Q

A

A

A

R

R

N

Q

Q

T

G

G

C

V

E

R

T

K

V

I

V

V

Y

H

A
x
T

S

S

T
x
S

-

G

S
x
G

S

S

I

I

Y

Y

G

G

N

T

R

P

T

P

E

E

-

Y

P

P

S

T

P

P

E

E

D

T

M

A

D

P

V

T

Q

D

A

P

N

A

T

S

G

P

Y
|
Y

E

A

A

A

S

G

K
|
K

L

V

A

A

R

G

E

E

R

I

Y

Y

A

L

E

N

Y

T

Y

F

S

R

N

H

F

L

H

Y

D

G

M

L

T

D

C

C

A

S

G

H

M

I

R

A

F
x
P

F
x
A

S
x
N

V
|
V

F

-

Q

-

G

-

Y

Y

G

G

G

P

S

R

E

Q

A

D

H

P

K

H

G

G

E

E

Y

-

A
|
A

N
x
G

V
|
V

I

V

A

A

Q

I

F
|
F

A

A

D

Q

S

A

L

L

A

L

T

S

G

G

E

K

T

P

P

T

V
x
R

L
x
V

Y
x
F

G

G

D

D

G

G

T

T

Q

N

T

T

R
|
R

D

D

F

Y

T

V

H

F

V

V

Q

D

D

D

I

V

V

V

R

D

G

A

L

F

E

V

I

R

A

V

A

S

D

A

E

D

R

V

L

G

D

G

G

G

I

L

-

R

Y

F

N

N

L

I

G

G

T

T

G

G

E

K

S

E

Y

T

S

S

F
x
D

N

R

T

Q

I

L

I

H

E

S

L

A

L

V

N

A

D

A

E

A

L

V

G

G

T

G

D

-

I

-

E

-

P

P

D

D

H

D

V

P

E

E

N

F

P

H

I

P

P

P

E
x
R

D

L

V

G

Y

D

V

L

H

K

D

R

T

S

M

C

A

L

D

D

I

I

T

G

K

L

M

A

T

E

T

R

E

V

T

L

S

G

W

W

E

R

P

P

Q

Q

I

I

T

E

F

L

E

A

E

D

G

G

I

V

R

R

L

R

V

T

T

V

N

E

E

Y

Y

F

R

R

N

H

E

K

binding nicotinamide-adenine-dinucleotide: G7 (= G10), G10 (= G13), F11 (= F14), I12 (= I15), D31 (= D33), N32 (= N34), T35 (≠ L37), G36 (= G38), D56 (≠ S53), I57 (≠ V54), L77 (= L69), A78 (= A70), A79 (= A71), I81 (≠ S73), V96 (= V88), T119 (≠ A111), Y146 (= Y136), K150 (= K140), P173 (≠ F163), A174 (≠ F164), N175 (≠ S165), V176 (= V166)
binding uridine-5'-diphosphate-glucose: I81 (≠ S73), R84 (≠ A76), S121 (≠ T113), G123 (≠ S114), Y146 (= Y136), A174 (≠ F164), N175 (≠ S165), A187 (= A181), G188 (≠ N182), V189 (= V183), F193 (= F187), R204 (≠ V198), V205 (≠ L199), F206 (≠ Y200), R213 (= R207), D248 (≠ F241), R271 (≠ E267)

7ysmA Crystal structure of udp-glucose 4-epimerase (rv3634c) co-crystallized with udp-n-acetylglucosamine from mycobacterium tuberculosis
34% identity, 96% coverage: 5:307/316 of query aligns to 2:311/311 of 7ysmA

query
sites
7ysmA

R

R

V

A

L

L

V

V

T

T

G
|
G

G

A

A

A

G
|
G

F
|
F

I
|
I

G

G

S

S

N

T

L

L

A

V

N

D

H

R

L

L

-

L

A

A

E

D

S

G

N

H

D

S

V

V

I

V

A

G

L

L

D
|
D

N
|
N

G

F

Y

A

L
x
T

G
|
G

T

R

E

A

E

T

N

N

L

L

-

E

-

H

-

L

-

A

-

D

N

N

N

S

K

A

V

H

E

V

F

F

V

V

E

E

A

A

S
x
D

V
x
I

L

V

D

T

D

A

D

D

L

L

P

H

T

A

-

I

-

L

-

E

-

Q

-

H

N

R

V

P

D

E

V

V

V

V

F

F

H

H

L
|
L

A
|
A

A
|
A

L

Q

S
x
I

S

D

Y

V

A
x
R

M

R

H

S

E

V

A

A

N

D

P

P

Q

Q

Q

F

G

D

A

A

R

A

V

V

N

N

V

V

K

I

G

G

F

T

V

V

N

R

V

L

V

A

E

E

Q

A

A

A

R

R

N

Q

Q

T

G

G

C

V

E

R

T

K

V

I

V

V

Y

H

A
x
T

S

S

T
x
S

-

G

S
x
G

S
|
S

I

I

Y

Y

G

G

N

T

R

P

T

P

E

E

-

Y

P

P

S

T

P

P

E

E

D

T

M

A

D

P

V

T

Q

D

A

P

N

A

T

S

G

P

Y
|
Y

E

A

A

A

S

G

K
|
K

L

V

A

A

R

G

E

E

R

I

Y

Y

A

L

E

N

Y

T

Y

F

S

R

N

H

F

L

H

Y

D

G

M

L

T

D

C

C

A

S

G

H

M

I

R

A

F
x
P

F
x
A

S
x
N

V
|
V

F

-

Q

-

G

-

Y

Y

G

G

G

P

S

R

E

Q

A

D

H

P

K

H

G

G

E

E

Y

-

A

A

N
x
G

V
|
V

I

V

A

A

Q

I

F
|
F

A

A

D

Q

S

A

L

L

A

L

T

S

G

G

E

K

T

P

P

T

V
x
R

L
x
V

Y
x
F

G

G

D

D

G

G

T

T

Q
x
N

T

T

R
|
R

D

D

F

Y

T

V

H

F

V

V

Q

D

D

D

I

V

V

V

R

D

G

A

L

F

E

V

I

R

A

V

A

S

D

A

E

D

R

V

L

G

D

G

G

G

I

L

-

R

Y

F

N

N

L

I

G

G

T

T

G

G

E

K

S

E

Y

T

S

S

F
x
D

N

R

T

Q

I

L

I

H

E

S

L

A

L

V

N

A

D

A

E

A

L

V

G

G

T

G

D

-

I

-

E

-

P

P

D

D

H

D

V

P

E

E

N

F

P

H

I

P

P

P

E
x
R

D

L

V

G

Y

D

V

L

H

K

D

R

T

S

M

C

A

L

D

D

I

I

T

G

K

L

M

A

T

E

T

R

E

V

T

L

S

G

W

W

E

R

P

P

Q

Q

I

I

T

E

F

L

E

A

E

D

G

G

I

V

R

R

L

R

V

T

T

V

N

E

E

Y

Y

F

R

R

N

H

E

K

binding nicotinamide-adenine-dinucleotide: G7 (= G10), G10 (= G13), F11 (= F14), I12 (= I15), D31 (= D33), N32 (= N34), T35 (≠ L37), G36 (= G38), D56 (≠ S53), I57 (≠ V54), L77 (= L69), A78 (= A70), A79 (= A71), I81 (≠ S73), T119 (≠ A111), Y146 (= Y136), K150 (= K140), P173 (≠ F163), N175 (≠ S165), V176 (= V166)
binding uridine-diphosphate-n-acetylgalactosamine: I81 (≠ S73), R84 (≠ A76), S121 (≠ T113), G123 (≠ S114), S124 (= S115), Y146 (= Y136), A174 (≠ F164), N175 (≠ S165), G188 (≠ N182), V189 (= V183), F193 (= F187), R204 (≠ V198), V205 (≠ L199), F206 (≠ Y200), N211 (≠ Q205), R213 (= R207), D248 (≠ F241), R271 (≠ E267)

3ruhA Alternative analogs as viable substrates of udp-hexose 4-epimerases
30% identity, 95% coverage: 4:304/316 of query aligns to 17:331/336 of 3ruhA

query
sites
3ruhA

K

K

R

T

V

W

L

L

V

I

T

T

G
|
G

G

V

A

A

G
|
G

F
|
F

I
|
I

G

G

S

S

N

N

L

L

A

L

N

E

H

K

L

L

A

L

E

K

S

L

N

N

D

Q

V

V

-

V

I

I

A

G

L

L

D
|
D

N
|
N

G

F

Y
x
S

L
x
T

G
|
G

T

H

E

Q

E

Y

N

N

L

L

N

D

-

E

-

V

-

K

-

T

-

L

-

V

-

S

-

T

-

E

-

Q

-

W

N

S

K

R

V

F

E

C

F

F

V

I

E

E

A

G

S
x
D

V
x
I

L

R

D

D

-

L

-

T

-

T

-

C

D

E

D

Q

L

V

P

M

T

K

N

G

V

V

D

D

V

H

V

V

F

L

H

H

L
x
Q

A
|
A

A
|
A

L

L

S
x
G

S
|
S

Y

V

A

P

M

R

H

S

E

I

A

V

N

D

P

P

Q

I

Q

T

G

T

A

N

R

A

V
x
T

N

N

V

I

K

T

G

G

F

F

V

L

N

N

V

I

V

L

E

H

Q

A

A

A

R

K

N

N

Q

A

G

Q

C

V

E

Q

T

S

V

F

V

T

Y

Y

A
|
A

S
x
A

T
x
S

S
|
S

S
|
S

I

T

Y

Y

G

G

N

D

R

H

T

-

E

-

P

-

S

-

P

P

E

A

D

L

M

P

D

K

V

V

Q

E

A

E

N

N

T

I

G

G

-

N

-

P

-

L

-

S

-

P

Y
|
Y

E

A

A

V

S

T

K
|
K

L

Y

A

V

R

N

E

E

R

I

Y

Y

A

A

E

Q

Y

V

Y

Y

S

A

N

R

F

T

H

Y

D

G

M

F

T

K

C

T

A

I

G

G

M

L

R

R

F
x
Y

F

F

S
x
N

V
|
V

F

F

Q

-

G

-

Y

-

G

G

G

R

S

R

E

Q

A

D

H

P

K
x
N

G

G

E

A

Y

Y

A

A

N
x
A

V
|
V

I

I

A

P

Q
x
K

F
x
W

A

T

D

A

S

A

L

M

A

L

T

K

G

G

E

D

T

D

P

V

V
x
Y

L
x
I

Y
x
N

G

G

D

D

G

G

T

E

Q

T

T

S

R
|
R

D

D

F

F

T

C

H

Y

V

I

Q

D

D

N

I

V

V

I

R

Q

G

M

-

N

-

I

-

L

L

S

E

A

I

L

A

A

A

K

D

D

E

S

R

A

L

K

D

D

G

N

I

I

Y

Y

N

N

L

V

G

A

T

V

G

G

E

D

S

R

Y

T

S

T

F
x
L

N

N

T

E

I

L

I

S

E

G

L

Y

L

I

N

Y

D

D

E

E

L

L

G

N

T

L

D

-

I

-

E

-

P

I

D

H

H

H

V

I

E

K

N

Y

P

R

I

E

P

F

E
x
R

D

S

V

G

Y
x
D

V
|
V

H

R

D

H

T
x
S

M

Q

A

A

D

D

I

V

T

T

K

K

M

A

T

I

T

D

E

L

T

L

S

K

W

Y

E

R

P

P

Q

N

I

I

T

K

F

I

E

R

E

E

G

G

I

L

R

R

L

L

V

S

T

M

N

P

E

W

Y

Y

active site: S142 (≠ T113), S143 (= S114), S144 (= S115), Y166 (= Y136), K170 (= K140), N204 (≠ K177)
binding nicotinamide-adenine-dinucleotide: G23 (= G10), G26 (= G13), F27 (= F14), I28 (= I15), D47 (= D33), N48 (= N34), S50 (≠ Y36), T51 (≠ L37), G52 (= G38), D78 (≠ S53), I79 (≠ V54), Q98 (≠ L69), A99 (= A70), A100 (= A71), T117 (≠ V88), A140 (= A111), A141 (≠ S112), S142 (≠ T113), Y166 (= Y136), K170 (= K140), Y193 (≠ F163), V196 (= V166)
binding [[(2R,3S,4R,5R)-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl][(2R,3R,4R,5R,6R)-6-(hydroxymethyl)-4,5-bis(oxidanyl)-3-(2-oxidanylidenepropyl)oxan-2-yl] hydrogen phosphate: G102 (≠ S73), S103 (= S74), S142 (≠ T113), S143 (= S114), S144 (= S115), Y166 (= Y136), Y193 (≠ F163), N195 (≠ S165), A209 (≠ N182), V210 (= V183), K213 (≠ Q186), W214 (≠ F187), Y225 (≠ V198), I226 (≠ L199), N227 (≠ Y200), R234 (= R207), L271 (≠ F241), R294 (≠ E267), D297 (≠ Y270), V298 (= V271), S301 (≠ T274)

3rufA Alternative analogs as viable substrates of udp-hexose 4-epimerases
30% identity, 95% coverage: 4:304/316 of query aligns to 17:331/336 of 3rufA

query
sites
3rufA

K

K

R

T

V

W

L

L

V

I

T

T

G
|
G

G

V

A

A

G
|
G

F
|
F

I
|
I

G

G

S

S

N

N

L

L

A

L

N

E

H

K

L

L

A

L

E

K

S

L

N

N

D

Q

V

V

-

V

I

I

A

G

L

L

D
|
D

N
|
N

G

F

Y
x
S

L
x
T

G
|
G

T

H

E

Q

E

Y

N

N

L

L

N

D

-

E

-

V

-

K

-

T

-

L

-

V

-

S

-

T

-

E

-

Q

-

W

N

S

K

R

V

F

E

C

F

F

V

I

E

E

A

G

S
x
D

V
x
I

L

R

D

D

-

L

-

T

-

T

-

C

D

E

D

Q

L

V

P

M

T

K

N

G

V

V

D

D

V

H

V

V

F

L

H

H

L
x
Q

A
|
A

A
|
A

L

L

S

G

S

S

Y

V

A

P

M

R

H

S

E

I

A

V

N

D

P

P

Q

I

Q

T

G

T

A

N

R

A

V
x
T

N

N

V

I

K

T

G

G

F

F

V

L

N

N

V

I

V

L

E

H

Q

A

A

A

R

K

N

N

Q

A

G

Q

C

V

E

Q

T

S

V

F

V

T

Y

Y

A
|
A

S

A

T
x
S

S
|
S

S
|
S

I

T

Y

Y

G

G

N

D

R

H

T

-

E

-

P

-

S

-

P

P

E

A

D

L

M

P

D

K

V

V

Q

E

A

E

N

N

T

I

G

G

-

N

-

P

-

L

-

S

-

P

Y
|
Y

E

A

A

V

S

T

K
|
K

L

Y

A

V

R

N

E

E

R

I

Y

Y

A

A

E

Q

Y

V

Y

Y

S

A

N

R

F

T

H

Y

D

G

M

F

T

K

C

T

A

I

G

G

M

L

R

R

F
x
Y

F

F

S
x
N

V
|
V

F

F

Q

-

G

-

Y

-

G

G

G

R

S

R

E

Q

A

D

H

P

K
x
N

G

G

E

A

Y

Y

A

A

N
x
A

V
|
V

I

I

A

P

Q
x
K

F
x
W

A

T

D

A

S

A

L

M

A

L

T

K

G

G

E

D

T

D

P

V

V
x
Y

L
x
I

Y
x
N

G

G

D

D

G

G

T

E

Q

T

T

S

R
|
R

D

D

F

F

T

C

H

Y

V

I

Q

D

D

N

I

V

V

I

R

Q

G

M

-

N

-

I

-

L

L

S

E

A

I

L

A

A

A

K

D

D

E

S

R

A

L

K

D

D

G

N

I

I

Y

Y

N

N

L

V

G

A

T

V

G

G

E

D

S

R

Y

T

S

T

F
x
L

N

N

T

E

I

L

I

S

E

G

L

Y

L

I

N

Y

D

D

E

E

L

L

G

N

T

L

D

-

I

-

E

-

P

I

D

H

H

H

V

I

E

K

N

Y

P

R

I

E

P

F

E
x
R

D

S

V

G

Y
x
D

V

V

H

R

D

H

T

S

M

Q

A

A

D

D

I

V

T

T

K

K

M

A

T

I

T

D

E

L

T

L

S

K

W

Y

E

R

P

P

Q

N

I

I

T

K

F

I

E

R

E

E

G

G

I

L

R

R

L

L

V

S

T

M

N

P

E

W

Y

Y

active site: S142 (≠ T113), S143 (= S114), S144 (= S115), Y166 (= Y136), K170 (= K140), N204 (≠ K177)
binding nicotinamide-adenine-dinucleotide: G23 (= G10), G26 (= G13), F27 (= F14), I28 (= I15), D47 (= D33), N48 (= N34), S50 (≠ Y36), T51 (≠ L37), G52 (= G38), D78 (≠ S53), I79 (≠ V54), Q98 (≠ L69), A99 (= A70), A100 (= A71), T117 (≠ V88), A140 (= A111), Y166 (= Y136), K170 (= K140), Y193 (≠ F163), V196 (= V166)
binding uridine-5'-diphosphate: N195 (≠ S165), A209 (≠ N182), V210 (= V183), K213 (≠ Q186), W214 (≠ F187), Y225 (≠ V198), I226 (≠ L199), N227 (≠ Y200), R234 (= R207), L271 (≠ F241), R294 (≠ E267), D297 (≠ Y270)

3lu1A Crystal structure analysis of wbgu: a udp-galnac 4-epimerase (see paper)
30% identity, 95% coverage: 4:304/316 of query aligns to 17:331/336 of 3lu1A

query
sites
3lu1A

K

K

R

T

V

W

L

L

V

I

T

T

G
|
G

G

V

A

A

G
|
G

F
|
F

I
|
I

G

G

S

S

N

N

L

L

A

L

N

E

H

K

L

L

A

L

E

K

S

L

N

N

D

Q

V

V

-

V

I

I

A

G

L

L

D
|
D

N
|
N

G

F

Y
x
S

L
x
T

G
|
G

T

H

E

Q

E

Y

N

N

L

L

N

D

-

E

-

V

-

K

-

T

-

L

-

V

-

S

-

T

-

E

-

Q

-

W

N

S

K

R

V

F

E

C

F

F

V

I

E

E

A

G

S
x
D

V
x
I

L

R

D

D

-

L

-

T

-

T

-

C

D

E

D

Q

L

V

P

M

T

K

N

G

V

V

D

D

V

H

V

V

F

L

H

H

L
x
Q

A
|
A

A
|
A

L

L

S

G

S
|
S

Y

V

A

P

M

R

H

S

E

I

A

V

N

D

P

P

Q

I

Q

T

G

T

A

N

R

A

V

T

N

N

V

I

K

T

G

G

F

F

V

L

N

N

V

I

V

L

E

H

Q

A

A

A

R

K

N

N

Q

A

G

Q

C

V

E
x
Q

T

S

V

F

V

T

Y

Y

A
|
A

S
x
A

T
x
S

S
|
S

S
|
S

I

T

Y

Y

G

G

N

D

R

H

T

-

E

-

P

-

S

-

P

P

E

A

D

L

M

P

D

K

V

V

Q

E

A

E

N

N

T

I

G

G

-

N

-

P

-

L

-

S

-

P

Y
|
Y

E

A

A

V

S

T

K
|
K

L

Y

A

V

R

N

E

E

R

I

Y

Y

A

A

E

Q

Y

V

Y

Y

S

A

N

R

F

T

H

Y

D

G

M

F

T
x
K

C

T

A

I

G

G

M

L

R

R

F
x
Y

F

F

S
x
N

V

V

F

F

Q

-

G

-

Y

-

G

G

G

R

S

R

E

Q

A

D

H

P

K
x
N

G

G

E

A

Y

Y

A

A

N

A

V
|
V

I

I

A

P

Q

K

F
x
W

A

T

D

A

S

A

L

M

A

L

T

K

G

G

E

D

T

D

P

V

V
x
Y

L
x
I

Y
x
N

G

G

D

D

G

G

T

E

Q

T

T

S

R
|
R

D

D

F

F

T

C

H

Y

V

I

Q

D

D

N

I

V

V

I

R

Q

G

M

-

N

-

I

-

L

L

S

E

A

I

L

A

A

A

K

D

D

E

S

R

A

L

K

D

D

G

N

I

I

Y

Y

N

N

L

V

G

A

T

V

G

G

E

D

S

R

Y

T

S

T

F
x
L

N

N

T

E

I

L

I

S

E

G

L

Y

L

I

N

Y

D

D

E

E

L

L

G

N

T

L

D

-

I

-

E

-

P

I

D

H

H

H

V

I

E

K

N

Y

P

R

I

E

P

F

E
x
R

D

S

V

G

Y
x
D

V

V

H

R

D

H

T

S

M

Q

A

A

D

D

I

V

T

T

K

K

M

A

T

I

T

D

E

L

T

L

S

K

W

Y

E

R

P

P

Q

N

I

I

T

K

F

I

E

R

E

E

G

G

I

L

R

R

L

L

V

S

T

M

N

P

E

W

Y

Y

active site: S142 (≠ T113), S143 (= S114), S144 (= S115), Y166 (= Y136), K170 (= K140), N204 (≠ K177)
binding glycine: Q135 (≠ E106), K187 (≠ T157)
binding nicotinamide-adenine-dinucleotide: G23 (= G10), G26 (= G13), F27 (= F14), I28 (= I15), D47 (= D33), N48 (= N34), S50 (≠ Y36), T51 (≠ L37), G52 (= G38), D78 (≠ S53), I79 (≠ V54), Q98 (≠ L69), A99 (= A70), A100 (= A71), A140 (= A111), A141 (≠ S112), S142 (≠ T113), Y166 (= Y136), K170 (= K140), Y193 (≠ F163), N195 (≠ S165)
binding uridine-diphosphate-n-acetylgalactosamine: S103 (= S74), S142 (≠ T113), S143 (= S114), S144 (= S115), Y166 (= Y136), N195 (≠ S165), V210 (= V183), W214 (≠ F187), Y225 (≠ V198), I226 (≠ L199), N227 (≠ Y200), R234 (= R207), L271 (≠ F241), R294 (≠ E267), D297 (≠ Y270)

7ys9A Crystal structure of udp-glucose 4-epimerase (rv3634c) in complex with both udp-glucose and udp-galactose in chaina from mycobacterium tuberculosis
34% identity, 96% coverage: 5:306/316 of query aligns to 2:310/310 of 7ys9A

query
sites
7ys9A

R

R

V

A

L

L

V

V

T

T

G
|
G

G

A

A

A

G
|
G

F
|
F

I
|
I

G

G

S

S

N

T

L

L

A

V

N

D

H

R

L

L

-

L

A

A

E

D

S

G

N

H

D

S

V

V

I

V

A

G

L

L

D
|
D

N
|
N

G

F

Y
x
A

L
x
T

G
|
G

T

R

E

A

E

T

N

N

L

L

-

E

-

H

-

L

-

A

-

D

N

N

N

S

K

A

V

H

E

V

F

F

V

V

E

E

A

A

S
x
D

V
x
I

L

V

D

T

D

A

D

D

L

L

P

H

T

A

-

I

-

L

-

E

-

Q

-

H

N

R

V

P

D

E

V

V

V

V

F

F

H

H

L
|
L

A
|
A

A
|
A

L

Q

S
x
I

S

D

Y

V

A
x
R

M

R

H

S

E

V

A

A

N

D

P

P

Q

Q

Q

F

G

D

A

A

R

A

V

V

N

N

V

V

K

I

G

G

F

T

V

V

N

R

V

L

V

A

E

E

Q

A

A

A

R

R

N

Q

Q

T

G

G

C

V

E

R

T

K

V

I

V

V

Y

H

A
x
T

S

S

T
x
S

-

G

S
x
G

S

S

I

I

Y

Y

G

G

N

T

R

P

T

P

E

E

-

Y

P

P

S

T

P

P

E

E

D

T

M

A

D

P

V

T

Q

D

A

P

N

A

T

S

G

P

Y
|
Y

E

A

A

A

S

G

K
|
K

L

V

A

A

R

G

E

E

R

I

Y

Y

A

L

E

N

Y

T

Y

F

S

R

N

H

F

L

H

Y

D

G

M

L

T

D

C

C

A

S

G

H

M

I

R

A

F
x
P

F
x
A

S
x
N

V
|
V

F

-

Q

-

G

-

Y

Y

G

G

G

P

S

R

E

Q

A

D

H

P

K

H

G

G

E

E

Y

-

A
|
A

N
x
G

V
|
V

I

V

A

A

Q

I

F
|
F

A

A

D

Q

S

A

L

L

A

L

T

S

G

G

E

K

T

P

P

T

V
x
R

L

V

Y
x
F

G

G

D

D

G

G

T

T

Q
x
N

T

T

R
|
R

D

D

F

Y

T

V

H

F

V

V

Q

D

D

D

I

V

V

V

R

D

G

A

L

F

E

V

I

R

A

V

A

S

D

A

E

D

R

V

L

G

D

G

G

G

I

L

-

R

Y

F

N

N

L

I

G

G

T

T

G

G

E

K

S

E

Y

T

S

S