SitesBLAST
Comparing WP_007695194.1 NCBI__GCF_000336675.1:WP_007695194.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
2c2bA Crystallographic structure of arabidopsis thaliana threonine synthase complexed with pyridoxal phosphate and s-adenosylmethionine (see paper)
29% identity, 78% coverage: 79:354/355 of query aligns to 118:402/444 of 2c2bA
- binding pyridoxal-5'-phosphate: F127 (= F93), K128 (= K94), D159 (≠ N120), G259 (≠ L220), G260 (= G221), N261 (≠ H222), L262 (≠ G223), G263 (≠ T224), N264 (≠ L225), A321 (≠ G273), H369 (≠ T321), T397 (= T349)
- binding s-adenosylmethionine: Q246 (= Q207), F247 (≠ R208)
Sites not aligning to the query:
- binding s-adenosylmethionine: 64, 65, 66, 67, 69, 90, 92, 97, 98, 100, 115, 115
2zsjA Crystal structure of threonine synthase from aquifex aeolicus vf5
30% identity, 85% coverage: 53:355/355 of query aligns to 9:323/350 of 2zsjA
- active site: K61 (= K94), T85 (≠ S118), Q218 (= Q252), A222 (≠ Y256), A240 (≠ G273), T317 (= T349)
- binding pyridoxal-5'-phosphate: F60 (= F93), K61 (= K94), N87 (= N120), V186 (≠ L220), G187 (= G221), N188 (≠ H222), A189 (≠ G223), G190 (≠ T224), N191 (≠ L225), A240 (≠ G273), T317 (= T349), G318 (= G350)
Q9S7B5 Threonine synthase 1, chloroplastic; Protein METHIONINE OVER-ACCUMULATOR 2; EC 4.2.3.1 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
29% identity, 78% coverage: 79:354/355 of query aligns to 193:477/526 of Q9S7B5
- L205 (≠ R96) mutation to R: In mto2-1; causes a strong decrease in the concentration of soluble threonine and over-accumulation of methionine.
Sites not aligning to the query:
- 172 binding S-adenosyl-L-methionine
- 173 binding S-adenosyl-L-methionine
- 181 binding in monomer B; binding in monomer A
- 187 binding in monomer B
P9WG59 Threonine synthase; TS; EC 4.2.3.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
31% identity, 87% coverage: 47:355/355 of query aligns to 20:332/360 of P9WG59
- K69 (= K94) modified: N6-(pyridoxal phosphate)lysine
- N95 (= N120) binding pyridoxal 5'-phosphate
- K151 (≠ D175) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
- GNAGN 196:200 (≠ GHGTL 221:225) binding pyridoxal 5'-phosphate
- T326 (= T349) binding pyridoxal 5'-phosphate
2d1fA Structure of mycobacterium tuberculosis threonine synthase (see paper)
31% identity, 87% coverage: 47:355/355 of query aligns to 11:323/349 of 2d1fA
- active site: K60 (= K94), T84 (≠ S118), D209 (≠ R243), R213 (= R247), L215 (= L249), A240 (≠ G273), T317 (= T349)
- binding pyridoxal-5'-phosphate: F59 (= F93), K60 (= K94), N86 (= N120), V186 (≠ L220), G187 (= G221), N188 (≠ H222), A189 (≠ G223), G190 (≠ T224), N191 (≠ L225), A240 (≠ G273), T317 (= T349)
A0R220 Threonine synthase; TS; EC 4.2.3.1 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
31% identity, 82% coverage: 64:355/355 of query aligns to 33:332/360 of A0R220
- K151 (≠ D175) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
6nmxA Threonine synthase from bacillus subtilis atcc 6633 with plp and appa (see paper)
30% identity, 86% coverage: 50:355/355 of query aligns to 7:321/350 of 6nmxA
- active site: K60 (= K94), T84 (≠ S118), E216 (≠ Q252), S220 (≠ Y256), A238 (≠ G273), T315 (= T349)
- binding (2E,3Z)-2-{[(Z)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4(1H)-ylidene}methyl]imino}-5-phosphonopent-3-enoic acid: K60 (= K94), S83 (= S117), T84 (≠ S118), N86 (= N120), T87 (≠ A121), F133 (≠ R166), N153 (≠ H187), S154 (≠ A188), R159 (≠ F193), V185 (≠ L220), G186 (= G221), N187 (≠ H222), A188 (≠ G223), G189 (≠ T224), N190 (≠ L225), A238 (≠ G273), I239 (= I274), E285 (= E319), T315 (= T349)
6cgqA Threonine synthase from bacillus subtilis atcc 6633 with plp and plp- ala (see paper)
30% identity, 86% coverage: 50:355/355 of query aligns to 3:311/339 of 6cgqA
- active site: K56 (= K94), T80 (≠ S118), E206 (≠ Q252), S210 (≠ Y256), A228 (≠ G273), T305 (= T349)
- binding pyridoxal-5'-phosphate: F55 (= F93), K56 (= K94), N82 (= N120), V175 (≠ L220), G176 (= G221), N177 (≠ H222), A178 (≠ G223), G179 (≠ T224), N180 (≠ L225), A228 (≠ G273), E275 (= E319), T305 (= T349), G306 (= G350)
2c2gA Crystal structure of threonine synthase from arabidopsis thaliana in complex with its cofactor pyridoxal phosphate (see paper)
29% identity, 78% coverage: 79:354/355 of query aligns to 136:404/448 of 2c2gA
6cgqB Threonine synthase from bacillus subtilis atcc 6633 with plp and plp- ala (see paper)
30% identity, 86% coverage: 50:355/355 of query aligns to 5:319/345 of 6cgqB
- active site: K58 (= K94), T82 (≠ S118), E214 (≠ Q252), S218 (≠ Y256), A236 (≠ G273), T313 (= T349)
- binding (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-alanine: K58 (= K94), S81 (= S117), T82 (≠ S118), N84 (= N120), T85 (≠ A121), V183 (≠ L220), G184 (= G221), N185 (≠ H222), A186 (≠ G223), N188 (≠ L225), A236 (≠ G273), I237 (= I274), E283 (= E319), T313 (= T349)
- binding phosphate ion: K58 (= K94), T85 (≠ A121), N151 (≠ H187), S152 (≠ A188), R157 (≠ F193), N185 (≠ H222)
1uimA Crystal structure of threonine synthase from thermus thermophilus hb8, orthorhombic crystal form (see paper)
33% identity, 85% coverage: 53:355/355 of query aligns to 9:323/350 of 1uimA
- active site: K61 (= K94), T85 (≠ S118), P212 (= P246), G216 (= G250), Q218 (= Q252), A240 (≠ G273), T317 (= T349)
- binding pyridoxal-5'-phosphate: F60 (= F93), K61 (= K94), N87 (= N120), G187 (= G221), N188 (≠ H222), A189 (≠ G223), G190 (≠ T224), N191 (≠ L225), A240 (≠ G273), E287 (= E319), T317 (= T349), G318 (= G350)
3aexA Catalytic intermediate analogue of threonine synthase from thermus thermophilus hb8 (see paper)
33% identity, 85% coverage: 53:355/355 of query aligns to 9:323/351 of 3aexA
- active site: K61 (= K94), T85 (≠ S118), P212 (= P246), G216 (= G250), Q218 (= Q252), A240 (≠ G273), T317 (= T349)
- binding (3E)-4-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}-2-oxobut-3-enoic acid: K61 (= K94), S84 (= S117), T85 (≠ S118), N87 (= N120), T88 (≠ A121), V186 (≠ L220), G187 (= G221), N188 (≠ H222), A189 (≠ G223), G190 (≠ T224), N191 (≠ L225), A240 (≠ G273), I241 (= I274), E287 (= E319), T317 (= T349)
- binding phosphate ion: K61 (= K94), T88 (≠ A121), N154 (≠ H187), S155 (≠ A188), R160 (≠ F193), N188 (≠ H222)
1v7cA Crystal structure of threonine synthase from thermus thermophilus hb8 in complex with a substrate analogue (see paper)
33% identity, 85% coverage: 53:355/355 of query aligns to 9:323/351 of 1v7cA
- active site: K61 (= K94), T85 (≠ S118), P212 (= P246), G216 (= G250), Q218 (= Q252), A240 (≠ G273), T317 (= T349)
- binding (2e)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]-5-phosphonopent-2-enoic acid: K61 (= K94), S84 (= S117), T85 (≠ S118), N87 (= N120), T88 (≠ A121), F134 (≠ R166), N154 (≠ H187), S155 (≠ A188), R160 (≠ F193), V186 (≠ L220), G187 (= G221), N188 (≠ H222), A189 (≠ G223), G190 (≠ T224), N191 (≠ L225), A240 (≠ G273), I241 (= I274), E287 (= E319), T317 (= T349)
3aeyA Apo form of threonine synthase from thermus thermophilus hb8 (see paper)
33% identity, 85% coverage: 53:355/355 of query aligns to 8:322/350 of 3aeyA