SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_007695195.1 NCBI__GCF_000336675.1:WP_007695195.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4whxA X-ray crystal structure of an amino acid aminotransferase from burkholderia pseudomallei bound to the co-factor pyridoxal phosphate
51% identity, 97% coverage: 11:309/309 of query aligns to 9:306/306 of 4whxA

query
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4whxA

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active site: F37 (= F39), K160 (= K161), E194 (= E195), L217 (= L219)
binding alanine: Y97 (= Y98), K160 (= K161), A258 (= A261)

1iyeA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
50% identity, 97% coverage: 9:309/309 of query aligns to 3:303/304 of 1iyeA

query
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1iyeA

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active site: F33 (= F39), G35 (= G41), K156 (= K161), A157 (≠ T162), E190 (= E195), L214 (= L219)
binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: R56 (= R62), Y92 (= Y98), Y126 (= Y131), K156 (= K161), Y161 (= Y166), E190 (= E195), G193 (= G198), E194 (= E199), N195 (= N200), L214 (= L219), G216 (= G221), I217 (= I222), T218 (= T223), G253 (= G259), T254 (= T260), A255 (= A261)

1iydA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
50% identity, 97% coverage: 9:309/309 of query aligns to 3:303/304 of 1iydA

query
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1iydA

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active site: F33 (= F39), G35 (= G41), K156 (= K161), A157 (≠ T162), E190 (= E195), L214 (= L219)
binding glutaric acid: Y92 (= Y98), Y126 (= Y131), A255 (= A261)
binding pyridoxal-5'-phosphate: R56 (= R62), K156 (= K161), Y161 (= Y166), E190 (= E195), G193 (= G198), E194 (= E199), L214 (= L219), G216 (= G221), I217 (= I222), T218 (= T223), T254 (= T260)

1i1mA Crystal structure of escherichia coli branched-chain amino acid aminotransferase. (see paper)
50% identity, 97% coverage: 9:309/309 of query aligns to 3:303/304 of 1i1mA

query
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1i1mA

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active site: K156 (= K161)
binding 4-methyl valeric acid: Y92 (= Y98), K156 (= K161), T254 (= T260), A255 (= A261)
binding pyridoxal-5'-phosphate: R56 (= R62), K156 (= K161), Y161 (= Y166), E190 (= E195), G193 (= G198), E194 (= E199), L214 (= L219), G216 (= G221), I217 (= I222), T218 (= T223), G253 (= G259), T254 (= T260)

1i1lA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase. (see paper)
50% identity, 97% coverage: 9:309/309 of query aligns to 3:303/304 of 1i1lA

query
sites
1i1lA

D

D

T

Y

V

I

W

W

M

F

D

N

G

G

E

E

F

M

V

V

D

R

W

W

D

E

E

D

A

A

Q

K

V

V

H

H

V

V

L

M

T

S

H

H

G

A

L

L

H

H

Y

Y

G

G

S

T

G

S

V

V

F

F

E

E

G

G

V

I

R

R

C

C

Y

Y

D

D

T

S

E

H

E

K

G

G

P

P

A

V

I

V

F

F

R

R

W

H

D

R

E

E

H

H

L

M

D

Q

R
|
R

F

L

Y

H

N

D

S

S

A

A

K

K

P

I

Y

Y

D

R

M

F

E

P

I

V

D

S

H

Q

S

S

R

I

E

D

E

E

L

L

T

M

E

E

A

A

T

C

L

R

E

D

L

V

I

I

E

R

R

K

Q

N

D

N

L

L

E

T

S

S

C

A

Y
|
Y

I

I

R

R

P

P

I

L

A

I

F

F

Y

V

G

G

Y

D

N

V

S

G

L

M

G

G

V

V

S

N

P

P

-

P

Q

A

D

G

C

Y

P

S

T

T

Q

D

T

V

A

I

I

I

A

A

C

A

W

F

P

P

W

W

G

G

T

A

Y

Y

L

L

G

G

D

A

E

E

A

A

L

L

E

E

N

Q

G

G

V

I

D

D

V

A

M

M

V

V

S

S

W

W

R

N

K

R

H

A

A

A

S

P

S

N

Q

T

I

I

P

P

T

T

N

A

A

A

K
|
K

T

A

T

G

G

G

L

N

Y
|
Y

V

L

N

S

S

S

M

L

L

L

A

V

G

G

E

S

E

E

A

A

R

R

N

H

G

G

Y

Y

V

Q

E

E

A

G

I

I

V

A

L

L

N

D

K

V

E

N

G

G

N

Y

V

I

A

S

E
|
E

G

G

P

A

G
|
G

E

E

N

N

L

L

F

F

L

E

V

V

R

K

D

D

G

G

T

V

I

L

H

F

T

T

P

P

G

P

F

F

A

T

E

S

S

S

I

A

L

L

D

P

G
|
G

I
|
I

T
|
T

R

R

Q

D

S

A

V

I

T

K

L

L

A

A

R

K

E

E

R

L

G

G

Y

I

D

E

V

V

D

R

D

E

S

Q

A

V

S

-

I

L

S

S

R

R

G

E

E

S

L

L

Y

Y

T

L

A

A

D

D

E

E

L

V

F

F

F

M

T

S

G

G

T
|
T

A
|
A

A

A

E

E

I

I

T

T

P

P

I

V

R

R

S

S

V

V

D

D

D

G

T

I

A

Q

I

V

G

G

T

E

G

G

E

R

R

C

G

G

P

P

V

V

T

T

E

K

E

R

L

I

Q

Q

S

Q

A

A

F

F

D

G

L

L

V

F

E

T

R

G

R

E

T

T

D

E

D

D

H

K

D

W

E

G

W

W

F

L

T

D

H

Q

L

V

active site: K156 (= K161)
binding 2-methylleucine: Y92 (= Y98), K156 (= K161), T254 (= T260), A255 (= A261)
binding pyridoxal-5'-phosphate: R56 (= R62), K156 (= K161), Y161 (= Y166), E190 (= E195), G193 (= G198), G216 (= G221), I217 (= I222), T218 (= T223), T254 (= T260)

2ej0B Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase with pyridoxamine 5'-phosphate
47% identity, 99% coverage: 5:309/309 of query aligns to 1:304/305 of 2ej0B

query
sites
2ej0B

E

Q

M

I

D

K

V

A

D

G

T

L

V

I

W

W

M

M

D

N

G

G

E

A

F

F

V

V

D

P

W

Q

D

E

E

E

A

A

Q

K

V

T

H

S

V

V

L

L

T

S

H

H

G

A

L

L

H

H

Y

Y

G

G

S

T

G

S

V

V

F
|
F

E

E

G
|
G

V

I

R

R

C

A

Y

Y

D

E

T

T

E

A

E

K

G

G

P

P

A

A

I

I

F

F

R

R

W

L

D

K

E

E

H

H

L

V

D

K

R
|
R

F

F

Y

Y

N

N

S

S

A

A

K

K

P

V

Y

L

D

R

M

M

E

E

I

I

D

P

H

F

S

A

R

P

E

E

L

L

T

E

E

E

A

A

T

I

L

K

E

E

L

V

I

V

E

R

R

R

Q

N

D

G

L

Y

E

R

S

S

C

C

Y

Y

I

I

R

R

P

P

I

L

A

A

F

W

Y

M

G

G

Y

A

N

K

S

A

L

L

G

G

V

V

S

N

P

P

-

L

Q

P

D

N

C

N

P

P

T

A

Q

E

T

V

A

M

I

V

A

A

C

A

W

W

P

E

W

W

G

G

T

A

Y

Y

L

L

G

G

D

E

E

E

A

A

L

V

E

R

N

K

G

G

V

A

D

R

V

L

M

I

V

T

S

S

W

W

R

A

K

R

H

F

A

P

S

A

S

N

Q

V

I

M

P

P

T

G

N

K

A

A

K
|
K

T

V

T

G

G

G

L

N

Y
|
Y

V

V

N

N

S

S

M

A

L

L

A

A

G

K

E

M

E

E

A

A

R

V

R

A

N

A

G

G

Y

A

V

D

E

E

A

A

I

L

V

L

L

L

N

D

K

E

E

E

G

G

N

Y

V

V

A

A

E
|
E

G

G

P

S

G
|
G

E
|
E

N

N

L

L

F

F

L

F

V

V

R

R

D

D

G

G

T

V

I

I

H

Y

T

A

P

-

G

-

F

L

A

E

E

H

S

S

I

V

-

N

L
|
L

D

E

G
|
G

I
|
I

T
|
T

R

R

Q

D

S

S

V

V

V

I

T

R

L

I

A

A

R

K

E

D

R

L

G

G

Y

Y

D

E

V

V

D

-

D

Q

S

V

A

V

S

R

I

A

S

T

R

R

G

D

E

Q

L

L

Y

Y

T

M

A

A

D

D

E

E

L

V

F

F

F

M

T

T

G
|
G

T
|
T

A

A

E

E

I

V

T

T

P

P

I

V

R

S

S

M

V

I

D

D

D

W

T

R

A

P

I

I

G

G

T

K

G

G

E

T

R

A

G

G

P

P

V

V

T

A

E

L

E

R

L

L

Q

R

S

E

A

V

F

Y

F

L

D

E

L

A

V

V

E

T

R

G

R

R

T

R

D

P

D

E

H

Y

D

E

E

G

W

W

F

L

T

T

H

Y

L

V

active site: F35 (= F39), G37 (= G41), K158 (= K161), E192 (= E195), L215 (= L219)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R58 (= R62), Y163 (= Y166), E192 (= E195), G195 (= G198), E196 (= E199), L215 (= L219), G217 (= G221), I218 (= I222), T219 (= T223), G254 (= G259), T255 (= T260)

2ej3A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with gabapentin
45% identity, 99% coverage: 5:309/309 of query aligns to 1:296/297 of 2ej3A

query
sites
2ej3A

E

Q

M

I

D

K

V

A

D

G

T

L

V

I

W

W

M

M

D

N

G

G

E

A

F

F

V

V

D

P

W

Q

D

E

E

E

A

A

Q

K

V

T

H

S

V

V

L

L

T

S

H

H

G

A

L

L

H

H

Y

Y

G

G

S

T

G

S

V

V

F
|
F

E

E

G
|
G

V

I

R

R

C

A

Y

Y

D

E

T

T

E

A

E

K

G

G

P

P

A

A

I

I

F

F

R

R

W

L

D

K

E

E

H

H

L

V

D

K

R
|
R

F

F

Y

Y

N

N

S

S

A

A

K

K

P

V

Y

L

D

R

M

M

E

E

I

I

D

P

H

F

S

A

R

P

E

E

L

L

T

E

E

E

A

A

T

I

L

K

E

E

L

V

I

V

E

R

R

R

Q

N

D

G

L

Y

E

R

S

S

C

C

Y

Y

I

I

R

R

P

P

I

L

A

A

F

W

Y

M

G

G

Y

A

N

K

S

A

L

L

G

G

V

V

S

N

P

P

-

L

Q

P

D

N

C

N

P

P

T

A

Q

E

T

V

A

M

I

V

A

A

C

A

W

W

P

E

W

W

G

-

T

-

Y

-

L

-

G

-

D

-

E

-

A

-

L

V

E

R

N

K

G

G

V

A

D

R

V

L

M

I

V

T

S

S

W

W

R

A

K

R

H

F

A

P

S

A

S

N

Q

V

I

M

P

P

T

G

N

K

A

A

K
|
K

T

V

T

G

G

G

L

N

Y
|
Y

V

V

N

N

S

S

M

A

L

L

A

A

G

K

E

M

E

E

A

A

R

V

R

A

N

A

G

G

Y

A

V

D

E

E

A

A

I

L

V

L

L

L

N

D

K

E

E

E

G

G

N

Y

V

V

A

A

E
|
E

G

G

P

S

G
|
G

E

E

N

N

L

L

F

F

L

F

V

V

R

R

D

D

G

G

T

V

I

I

H

Y

T

-

P

-

G

A

F

L

A

E

E

H

S

S

I

V

-

N

L
|
L

D

E

G
|
G

I
|
I

T
|
T

R

R

Q

D

S

S

V

V

V

I

T

R

L

I

A

A

R

K

E

D

R

L

G

G

Y

Y

D

E

V

V

D

-

D

Q

S

V

A

V

S

R

I

A

S

T

R

R

G

D

E

Q

L

L

Y

Y

T

M

A

A

D

D

E

E

L

V

F

F

F

M

T

T

G
|
G

T
|
T

A
|
A

A

A

E

E

I

V

T

T

P

P

I

V

R

S

S

M

V

I

D

D

D

W

T

R

A

P

I

I

G

G

T

K

G

G

E

T

R

A

G

G

P

P

V

V

T

A

E

L

E

R

L

L

Q

R

S

E

A

V

F

Y

F

L

D

E

L

A

V

V

E

T

R

G

R

R

T

R

D

P

D

E

H

Y

D

E

E

G

W

W

F

L

T

T

H

Y

L

V

active site: F35 (= F39), G37 (= G41), K150 (= K161), E184 (= E195), L207 (= L219)
binding [1-(aminomethyl)cyclohexyl]acetic acid: G187 (= G198), G246 (= G259), T247 (= T260), A248 (= A261)
binding pyridoxal-5'-phosphate: R58 (= R62), K150 (= K161), Y155 (= Y166), E184 (= E195), G187 (= G198), L207 (= L219), G209 (= G221), I210 (= I222), T211 (= T223), G246 (= G259), T247 (= T260)

2eiyA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with 4-methylvaleric acid
45% identity, 99% coverage: 5:309/309 of query aligns to 1:296/297 of 2eiyA

query
sites
2eiyA

E

Q

M

I

D

K

V

A

D

G

T

L

V

I

W

W

M

M

D

N

G

G

E

A

F

F

V

V

D

P

W

Q

D

E

E

E

A

A

Q

K

V

T

H

S

V

V

L

L

T

S

H

H

G

A

L

L

H

H

Y

Y

G

G

S

T

G

S

V

V

F
|
F

E

E

G
|
G

V

I

R

R

C

A

Y

Y

D

E

T

T

E

A

E

K

G

G

P

P

A

A

I

I

F

F

R

R

W

L

D

K

E

E

H

H

L

V

D

K

R
|
R

F

F

Y

Y

N

N

S

S

A

A

K

K

P

V

Y

L

D

R

M

M

E

E

I

I

D

P

H

F

S

A

R

P

E

E

L

L

T

E

E

E

A

A

T

I

L

K

E

E

L

V

I

V

E

R

R

R

Q

N

D

G

L

Y

E

R

S

S

C

C

Y
|
Y

I

I

R

R

P

P

I

L

A

A

F

W

Y

M

G

G

Y

A

N

K

S

A

L

L

G

G

V

V

S

N

P

P

-

L

Q

P

D

N

C

N

P

P

T

A

Q

E

T

V

A

M

I

V

A

A

C

A

W

W

P

E

W

W

G

-

T

-

Y

-

L

-

G

-

D

-

E

-

A

-

L

V

E

R

N

K

G

G

V

A

D

R

V

L

M

I

V

T

S

S

W

W

R

A

K

R

H

F

A

P

S

A

S

N

Q

V

I

M

P

P

T

G

N

K

A

A

K
|
K

T

V

T

G

G

G

L

N

Y
|
Y

V

V

N

N

S

S

M

A

L

L

A

A

G

K

E

M

E

E

A

A

R

V

R

A

N

A

G

G

Y

A

V

D

E

E

A

A

I

L

V

L

L

L

N

D

K

E

E

E

G

G

N

Y

V

V

A

A

E
|
E

G

G

P

S

G
|
G

E
|
E

N

N

L

L

F

F

L

F

V

V

R

R

D

D

G

G

T

V

I

I

H

Y

T

-

P

-

G

A

F

L

A

E

E

H

S

S

I

V

-

N

L
|
L

D

E

G
|
G

I
|
I

T
|
T

R

R

Q

D

S

S

V

V

V

I

T

R

L

I

A

A

R

K

E

D

R

L

G

G

Y

Y

D

E

V

V

D

-

D

Q

S

V

A

V

S

R

I

A

S

T

R

R

G

D

E

Q

L

L

Y

Y

T

M

A

A

D

D

E

E

L

V

F

F

F

M

T

T

G
|
G

T
|
T

A
|
A

A

A

E

E

I

V

T

T

P

P

I

V

R

S

S

M

V

I

D

D

D

W

T

R

A

P

I

I

G

G

T

K

G

G

E

T

R

A

G

G

P

P

V

V

T

A

E

L

E

R

L

L

Q

R

S

E

A

V

F

Y

F

L

D

E

L

A

V

V

E

T

R

G

R

R

T

R

D

P

D

E

H

Y

D

E

E

G

W

W

F

L

T

T

H

Y

L

V

active site: F35 (= F39), G37 (= G41), K150 (= K161), E184 (= E195), L207 (= L219)
binding 4-methyl valeric acid: F35 (= F39), Y94 (= Y98), T247 (= T260), A248 (= A261)
binding pyridoxal-5'-phosphate: R58 (= R62), K150 (= K161), Y155 (= Y166), E184 (= E195), G187 (= G198), E188 (= E199), L207 (= L219), G209 (= G221), I210 (= I222), T211 (= T223), G246 (= G259), T247 (= T260)

1wrvA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase
45% identity, 99% coverage: 5:309/309 of query aligns to 1:296/297 of 1wrvA

query
sites
1wrvA

E

Q

M

I

D

K

V

A

D

G

T

L

V

I

W

W

M

M

D

N

G

G

E

A

F

F

V

V

D

P

W

Q

D

E

E

E

A

A

Q

K

V

T

H

S

V

V

L

L

T

S

H

H

G

A

L

L

H

H

Y

Y

G

G

S

T

G

S

V

V

F
|
F

E

E

G
|
G

V

I

R

R

C

A

Y

Y

D

E

T

T

E

A

E

K

G

G

P

P

A

A

I

I

F

F

R

R

W

L

D

K

E

E

H

H

L

V

D

K

R
|
R

F

F

Y

Y

N

N

S

S

A

A

K

K

P

V

Y

L

D

R

M

M

E

E

I

I

D

P

H

F

S

A

R

P

E

E

L

L

T

E

E

E

A

A

T

I

L

K

E

E

L

V

I

V

E

R

R

R

Q

N

D

G

L

Y

E

R

S

S

C

C

Y

Y

I

I

R

R

P

P

I

L

A

A

F

W

Y

M

G

G

Y

A

N

K

S

A

L

L

G

G

V

V

S

N

P

P

-

L

Q

P

D

N

C

N

P

P

T

A

Q

E

T

V

A

M

I

V

A

A

C

A

W

W

P

E

W

W

G

-

T

-

Y

-

L

-

G

-

D

-

E

-

A

-

L

V

E

R

N

K

G

G

V

A

D

R

V

L

M

I

V

T

S

S

W

W

R

A

K

R

H

F

A

P

S

A

S

N

Q

V

I

M

P

P

T

G

N

K

A

A

K
|
K

T

V

T

G

G

G

L

N

Y
|
Y

V

V

N

N

S

S

M

A

L

L

A

A

G

K

E

M

E

E

A

A

R

V

R

A

N

A

G

G

Y

A

V

D

E

E

A

A

I

L

V

L

L

L

N

D

K

E

E

E

G

G

N

Y

V

V

A

A

E
|
E

G

G

P

S

G
|
G

E

E

N

N

L

L

F

F

L

F

V

V

R

R

D

D

G

G

T

V

I

I

H

Y

T

-

P

-

G

A

F

L

A

E

E

H

S

S

I

V

-

N

L
|
L

D

E

G
|
G

I
|
I

T
|
T

R

R

Q

D

S

S

V

V

V

I

T

R

L

I

A

A

R

K

E

D

R

L

G

G

Y

Y

D

E

V

V

D

-

D

Q

S

V

A

V

S

R

I

A

S

T

R

R

G

D

E

Q

L

L

Y

Y

T

M

A

A

D

D

E

E

L

V

F

F

F

M

T

T

G

G

T
|
T

A

A

E

E

I

V

T

T

P

P

I

V

R

S

S

M

V

I

D

D

D

W

T

R

A

P

I

I

G

G

T

K

G

G

E

T

R

A

G

G

P

P

V

V

T

A

E

L

E

R

L

L

Q

R

S

E

A

V

F

Y

F

L

D

E

L

A

V

V

E

T

R

G

R

R

T

R

D

P

D

E

H

Y

D

E

E

G

W

W

F

L

T

T

H

Y

L

V

active site: F35 (= F39), G37 (= G41), K150 (= K161), E184 (= E195), L207 (= L219)
binding pyridoxal-5'-phosphate: R58 (= R62), K150 (= K161), Y155 (= Y166), E184 (= E195), G187 (= G198), L207 (= L219), G209 (= G221), I210 (= I222), T211 (= T223), T247 (= T260)

2ej2A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with n-(5'-phosphopyridoxyl)-l-glutamate
45% identity, 99% coverage: 5:309/309 of query aligns to 1:293/294 of 2ej2A

query
sites
2ej2A

E

Q

M

I

D

K

V

A

D

G

T

L

V

I

W

W

M

M

D

N

G

G

E

A

F

F

V

V

D

P

W

Q

D

E

E

E

A

A

Q

K

V

T

H

S

V

V

L

L

T

S

H

H

G

A

L

L

H

H

Y

Y

G

G

S

T

G

S

V

V

F
|
F

E

E

G
|
G

V

I

R

R

C

A

Y

Y

D

E

T

T

E

A

E

K

G

G

P

P

A

A

I

I

F

F

R

R

W

L

D

K

E

E

H

H

L

V

D

K

R
|
R

F

F

Y

Y

N

N

S

S

A

A

K

K

P

V

Y

L

D

R

M

M

E

E

I

I

D

P

H

F

S

A

R

P

E

E

L

L

T

E

E

E

A

A

T

I

L

K

E

E

L

V

I

V

E

R

R

R

Q

N

D

G

L

Y

E

R

S

S

C

C

Y
|
Y

I

I

R

R

P

P

I

L

A

A

F

W

Y

M

G

G

Y

A

N

K

S

A

L

L

G

G

V

V

S

N

P

P

-

L

Q

P

D

N

C

N

P

P

T

A

Q

E

T

V

A

M

I

V

A

A

C

A

W

W

P

E

W

W

G

G

T

A

Y

R

L

L

G

-

D

-

E

-

A

-

L

-

E

-

N

-

G

-

V

-

D

-

V

-

M

I

V

T

S

S

W

W

R

A

K

R

H

F

A

P

S

A

S

N

Q

V

I

M

P

P

T

G

N

K

A

A

K
|
K

T

V

T

G

G

G

L

N

Y
|
Y

V

V

N

N

S

S

M

A

L

L

A

A

G

K

E

M

E

E

A

A

R

V

R

A

N

A

G

G

Y

A

V

D

E

E

A

A

I

L

V

L

L

L

N

D

K

E

E

E

G

G

N

Y

V

V

A

A

E
|
E

G

G

P

S

G
|
G

E
|
E

N

N

L

L

F

F

L

F

V

V

R

R

D

D

G

G

T

V

I

I

H

Y

T

A

P

-

G

-

F

L

A

E

E

H

S

S

I

V

-

N

L
|
L

D

E

G
|
G

I
|
I

T
|
T

R

R

Q

D

S

S

V

V

V

I

T

R

L

I

A

A

R

K

E

D

R

L

G

G

Y

Y

D

E

V

V

D

-

D

Q

S

V

A

V

S

R

I

A

S

T

R

R

G

D

E

Q

L

L

Y

Y

T

M

A

A

D

D

E

E

L

V

F

F

F

M

T

T

G

G

T
|
T

A
|
A

A

A

E

E

I

V

T

T

P

P

I

V

R

S

S

M

V

I

D

D

D

W

T

R

A

P

I

I

G

G

T

K

G

G

E

T

R

A

G

G

P

P

V

V

T

A

E

L

E

R

L

L

Q

R

S

E

A

V

F

Y

F

L

D

E

L

A

V

V

E

T

R

G

R

R

T

R

D

P

D

E

H

Y

D

E

E

G

W

W

F

L

T

T

H

Y

L

V

active site: F35 (= F39), G37 (= G41), K147 (= K161), E181 (= E195), L204 (= L219)
binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: R58 (= R62), Y94 (= Y98), Y152 (= Y166), E181 (= E195), G184 (= G198), E185 (= E199), L204 (= L219), G206 (= G221), I207 (= I222), T208 (= T223), T244 (= T260), A245 (= A261)

6thqB Crystal structure of branched-chain aminotransferase from thermophilic archaea thermoproteus uzoniensis with norvaline
42% identity, 96% coverage: 11:307/309 of query aligns to 9:298/301 of 6thqB

query
sites
6thqB

V

V

W

W

M

L

D

D

G

G

E

R

F

L

V

V

D

D

W

E

D

E

E

E

A

A

Q

K

V

V

H

T

V

V

L

L

T

S

H

P

G

S

L

L

H

N

Y

Y

G

G

S

F

G

G

V

V

F
|
F

E

E

G

G

V

I

R

R

C

A

Y

Y

D

W

T

N

E

G

E

E

G

N

P

L

A

Y

I

V

F

F

R

R

W

L

D

R

E

D

H

H

L

M

D

E

R
|
R

F

L

Y

L

N

R

S

S

A

A

K

K

P

I

Y

I

D

G

M

L

E

D

I

V

D

P

H

Y

S

T

R

A

E

E

L

L

T

S

E

K

A

A

T

V

L

V

E

E

L

T

I

V

E

R

R

A

Q

N

D

G

L

F

-

K

E

E

S

D

C

L

Y
|
Y

I

I

R

R

P

P

I

V

A

A

F

Y

Y

I

G

S

Y

K

N

P

S

Q

L

I

G

S

V

L

S

D

P

V

Q

R

D

G

C

L

P

Q

T

A

Q

S

T

V

A

A

I

I

A

A

C

A

W

I

P

P

W

F

G

G

T

K

Y

Y

L

L

G

K

D

V

E

E

A

-

L

-

E

-

N

-

G

G

V

V

D

R

V

A

M

A

V

V

S

V

S

S

W

W

R

R

K

R

H

V

A

H

S

T

S

S

Q

M

I

M

P

P

T

V

N

M

A

A

K
|
K

T

A

T

T

G

G

L

I

Y
|
Y

V

L

N

N

S

S

M

I

L

M

A

A

G

A

E

V

E

E

A

A

R

R

R

A

N

R

G

G

Y

Y

V

D

E

E

A

A

I

I

V

M

L

L

N

N

K

A

E

E

G

G

N

K

V

V

A

V

E
|
E

G

G

P

S

G
|
G

E
|
E

N
|
N

L

I

F

F

L

I

V

V

R

R

D

R

G

G

T

V

I

L

H

M

T

T

P

P

G

P

F

L

A

E

E

D

S

G

I

I

L
|
L

D

E

G
|
G

I
|
I

T
|
T

R

R

Q

E

S

T

V

V

V

I

T

S

L

I

A

A

R

G

E

D

R

L

G

G

Y

I

D

P

V

L

D

L

D

E

S

K

A

-

S

S

I

I

S

T

R

R

G

E

E

E

L

L

Y

Y

T

A

A

A

D

D

E

E

L

A

F

F

T

V

G
|
G

T
|
T

A
|
A

A

A

E

E

I

I

T

T

P

P

I

I

R

I

S

E

V

I

D

D

D

G

T

R

A

V

I

L

G

-

T

-

G

-

E

Q

R

R

G

G

P

P

V

I

T

T

E

Q

E

K

L

I

Q

A

S

E

A

T

F

Y

F

R

D

R

L

I

V

V

E

L

R

G

R

K

T

E

D

E

D

K

H

Y

D

L

E

P

W

W

F

L

T

T

active site: F37 (= F39), K156 (= K161), E190 (= E195), L214 (= L219)
binding pyridoxal-5'-phosphate: R60 (= R62), K156 (= K161), Y161 (= Y166), E190 (= E195), N195 (= N200), L214 (= L219), G216 (= G221), I217 (= I222), T218 (= T223), T254 (= T260)
binding 2-[o-phosphonopyridoxyl]-amino-pentanoic acid: R60 (= R62), Y97 (= Y98), K156 (= K161), Y161 (= Y166), E190 (= E195), G193 (= G198), E194 (= E199), N195 (= N200), G216 (= G221), I217 (= I222), T218 (= T223), G253 (= G259), T254 (= T260), A255 (= A261)

5mr0D Thermophilic archaeal branched-chain amino acid transaminases from geoglobus acetivorans and archaeoglobus fulgidus: biochemical and structural characterisation (see paper)
41% identity, 93% coverage: 11:296/309 of query aligns to 4:282/290 of 5mr0D

query
sites
5mr0D

V

V

W

Y

M

M

D

D

G

G

E

E

F

F

V

V

D

P

W

E

D

N

E

E

A

A

Q

K

V

V

H

S

V

I

L

F

T

D

H

H

G

G

L

F

H

L

Y

Y

G

G

S

D

G

G

V

V

F
|
F

E

E

G
|
G

V

I

R

R

C

A

Y

Y

D

N

T

G

E

R

E

-

G

-

P

-

A

-

I

V

F

F

R

R

W

L

D

K

E

E

H

H

L

I

D

D

R
|
R

F

L

Y

Y

N

D

S

S

A

A

K

K

P

A

Y

I

D

D

M

L

E

E

I

I

D

P

H

I

S

T

R

K

E

E

L

F

T

M

E

E

A

I

T

I

L

L

E

E

L

T

I

L

E

R

R

K

Q

N

D

N

L

L

E

R

S

D

C

A

Y

Y

I

I

R

R

P

P

I

I

A

V

F

T

Y

R

G

G

Y

I

N

G

S

D

L

L

G
|
G

V
x
L

S

D

P

P

Q

R

D

K

C

C

P

Q

T

N

Q

P

T

S

A

I

I

I

A

V

C

I

-

T

W

K

P

P

W

W

G

G

T

K

Y

L

L

Y

G

G

D

D

E

-

A

L

L

Y

E

E

N

K

G

G

V

L

D

T

V

A

M

I

V

T

S

V

S

A

W

V

R

R

K

R

H

N

A

S

S

F

S

D

Q

A

I

L

P

P

T

P

N

N

A

I

K
|
K

T

S

T

L

G

N

L

-

Y
|
Y

V

L

N

N

S

N

M

I

L

L

A

A

G

K

E

I

E

E

A

A

R

N

R

A

N

K

G

G

Y

G

V

D

E

E

A

A

I

I

V

F

L

L

N

D

K

R

E

N

G

G

N

Y

V

V

A

S

E
|
E

G

G

P

S

G
|
G

E
x
D

N

N

L

I

F

F

L

V

V

V

R

K

D

N

G

G

T

A

I

I

H

T

T

T

P

P

G

P

F

T

A

I

E

N

S

N

I

-

L
|
L

D

R

G

G

I
|
I

T
|
T

R

R

Q

E

S

A

V

V

V

I

T

E

L

I

A

I

R

N

E

R

R

L

G

G

Y

I

D

P

V

F

D

K

D

E

S

T

A

-

S

N

I

I

S

G

R

L

G

Y

E

D

L

L

Y

Y

T

T

A

A

D

D

E

E

L

V

F

F

F

V

T

T

G
|
G

T
|
T

A

A

E

E

I

I

T

A

P

P

I

I

R

V

S

V

V

I

D

D

D

G

T

R

A

K

I

I

G

G

T

D

G

G

E

K

R

P

G

G

P

E

V

I

T

T

E

R

E

K

L

L

Q

M

S

E

A

E

F

F

F

S

D

K

L

L

V

T

E

E

active site: F32 (= F39), G34 (= G41), K150 (= K161), E183 (= E195), L206 (= L219)
binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: R51 (= R62), G100 (= G111), L101 (≠ V112), K150 (= K161), Y154 (= Y166), E183 (= E195), G186 (= G198), D187 (≠ E199), L206 (= L219), I209 (= I222), T210 (= T223), G245 (= G259), T246 (= T260)

5e25A Crystal structure of branched-chain aminotransferase from thermophilic archaea geoglobus acetivorans complexed with alpha-ketoglutarate (see paper)
42% identity, 91% coverage: 11:291/309 of query aligns to 5:278/290 of 5e25A

query
sites
5e25A

V

V

W

Y

M

M

D

N

G

G

E

E

F

F

V

V

D

P

W

E

D

S

E

Q

A

A

Q

K

V

V

H

S

V

V

L

F

T

D

H

H

G

G

L

F

H

L

Y

Y

G

G

S

D

G

G

V

V

F
|
F

E

E

G
|
G

V

I

R

R

C

A

Y

Y

D

N

T

G

E

K

E

-

G

-

P

-

A

-

I

V

F

F

R

K

W

L

D

Y

E

E

H

H

L

I

D

D

R
|
R

F

L

Y

Y

N

D

S

C

A

A

K

R

P

V

Y

I

D

D

M

L

E

K

I

I

D

P

H

L

S

S

R

K

E

E

L

F

T

A

E

E

A

A

T

I

L

L

E

E

L

T

I

L

E

R

R

R

Q

N

D

N

L

L

E

R

S

D

C

A

Y
|
Y

I

I

R

R

P

P

I

I

A

V

F

T

Y

R

G

G

Y

A

N

G

S

D

L

L

G

G

V

L

S

D

P

P

Q

R

D

K

C

C

P

P

T

S

-

P

Q

N

T

V

A

I

I

I

A

I

C

T

W

K

P

P

W

W

G

G

T

K

Y

L

L

Y

G

G

D

D

E

-

A

L

L

Y

E

E

N

K

G

G

V

L

D

K

V

A

M

I

V

T

S

V

S

A

W

I

R

R

K

R

H

N

A

A

S

I

S

D

Q

S

I

L

P

P

T

P

N

N

A

I

K
|
K

T

S

T

L

G

N

L

-

Y
|
Y

V

L

N

N

S

N

M

I

L

L

A

A

G

K

E

I

E

E

A

A

R

N

R

A

N

K

G

G

Y

G

V

D

E

E

A

A

I

I

V

F

L

L

N

D

K

H

E

N

G

G

N

Y

V

I

A

S

E
|
E

G

G

P

S

G
|
G

E
x
D

N

N

L

I

F

F

L

I

V

V

R

K

D

N

G

G

T

T

I

I

H

T

T

T

P

P

G

P

F

T

A

L

E

N

S

N

I

-

L
|
L

D

K

G
|
G

I
|
I

T
|
T

R

R

Q

Q

S

V

V

V

V

I

T

E

L

L

A

I

R

N

E

E

R

L

G

E

Y

I

D

P

V

F

D

R

D

E

S

-

A

A

S

N

I

I

S

G

R

L

G

F

E

D

L

L

Y

Y

T

S

A

A

D

D

E

E

L

I

F

F

F

V

T

T

G
|
G

T
|
T

A
|
A

A

A

E

E

I

I

T

A

P

P

I

V

R

T

S

Y

V

I

D

D

D

G

T

R

A

T

I

V

G

G

T

N

G

G

E

K

R

P

G

G

P

K

V

V

T

T

E

K

E

M

L

L

Q

M

S

E

A

K

F

F

active site: F33 (= F39), G35 (= G41), K151 (= K161), E184 (= E195), L207 (= L219)
binding 2-oxoglutaric acid: Y88 (= Y98), K151 (= K161), T247 (= T260), A248 (= A261)
binding pyridoxal-5'-phosphate: R52 (= R62), K151 (= K161), Y155 (= Y166), E184 (= E195), G187 (= G198), D188 (≠ E199), L207 (= L219), G209 (= G221), I210 (= I222), T211 (= T223), G246 (= G259), T247 (= T260)

6h65C Crystal structure of the branched-chain-amino-acid aminotransferase from haliangium ochraceum
33% identity, 95% coverage: 12:306/309 of query aligns to 8:303/308 of 6h65C

query
sites
6h65C

W

W

M

M

D

N

G

G

E

Q

F

C

V

I

D

P

W

W

D

D

E

Q

A

C

Q

S

V

L

H

H

V

V

L

S

T

T

H

Q

G

A

L

A

H

F

Y

F

G

G

S

A

G

S

V

L

F
|
F

E

E

G

G

V

V

R

R

C

A

Y

Y

-

W

-

N

D

A

T

E

E

R

E

E

G

Q

P

L

A

Y

I

V

F

F

R

R

W

L

D

D

E

E

H

H

L

L

D

R

R
|
R

F

L

Y

E

N

Q

S

S

A

A

K

K

P

M

Y

L

D

R

M

M

E

K

I

L

D

S

H

M

S

P

R

I

E

A

E

D

L

I

T

R

E

Q

A

G

T

V

L

L

E

E

L

L

I

L

E

R

R

A

Q

N

D

E

L

F

E

R

S

S

-

D

C

V

Y

H

I

L

R

Y

P

V

I

A

A

S

F

Y

Y

F

G

G

Y

I

N

N

S

H

L

-

G

-

V

-

S

D

P

P

Q

D

D

P

C

L

P

F

T

P

Q

T

T

D

A

D

I

T

A

G

C

V

W

Y

P

V

W

T

G

G

T

T

Y

A

L

V

G

S

D

R

E

L

A

P

L

L

-

V

E

H

N

T

G

G

V

I

D

S

V

A

M

C

V

M

S

S

W

W

R

R

K

R

H

I

A

S

S

D

Q

S

I

V

P

P

T

P

N

R

A

I

K
|
K

T

I

T

G

G

A

L

N

Y
|
Y

V

Q

N

N

S

S

M

R

L

L

A

A

G

Q

E

T

E

E

A

A

R

R

R

V

N

N

G

G

Y

Y

V

H

E

T

A

S

I

V

V

L

L

L

N

N

K

S

E

R

G

G

N

K

V

V

A

S

E
|
E

G

T

P

P

G

G

E
x
A

N

C

L

L

F

L

L

M

V

V

R

R

D

D

G

G

T

R

I

V

H

I

T

S

P

P

G

P

F

V

A

T

E

A

S

D

I

I

L
|
L

D

E

G
x
S

I
x
V

T
|
T

R

R

Q

K

S

T

V

L

V

M

T

S

L

L

A

S

R

E

E

A

R

E

G

L

Y

D

D

S

V

P

D

V

D

I

S

E

A

R

S

D

I

M

S

D

R

R

G

T

E

E

L

L

Y

Y

T

I

A

A

D

E

E

E

L

V

F

F

F

L

T

C

G
|
G

T
|
T

A

I

A

A

E

E

I

I

T

L

P

P

I

V

R

T

S

T

V

I

D

D

D

R

T

I

A

Q

I

V

G

G

T

D

G

G

E

E

R

V

G

G

P

P

V

V

T

T

E

R

L

L

Q

Q

S

E

A

L

F

Y

F

F

D

G

L

V

V

T

E

S

R

G

R

Q

T

L

D

E

D

A

H

Y

D

K

E

S

W

W

F

L

active site: F35 (= F39), K158 (= K161), E192 (= E195), L216 (= L219)
binding pyridoxal-5'-phosphate: R60 (= R62), K158 (= K161), Y163 (= Y166), E192 (= E195), A196 (≠ E199), L216 (= L219), S218 (≠ G221), V219 (≠ I222), T220 (= T223), G256 (= G259), T257 (= T260)

6q8eA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum in pmp-form (see paper)
35% identity, 96% coverage: 11:307/309 of query aligns to 6:302/307 of 6q8eA

query
sites
6q8eA

V

L

W

W

M

W

D

N

G

H

E

R

F

I

V

I

D

P

W

W

D

E

E

E

A

A

Q

T

V

V

H

H

V

L

L

T

T

D

H

Y

G

W

L

W

H

A

Y

S

G

V

S

T

G

A

V

V

F
|
F

E

E

G

G

V

I

R

R

C

G

Y

Y

-

W

D

N

T

N

E

A

E

E

G

G

P

E

A

M

-

Y

I

I

F

F

R

R

W

L

D

E

E

D

H

H

L

A

D

R

R
|
R

F

L

Y

E

N

Q

S

S

A

M

K

Q

P

L

Y

I

D

R

M

M

E

P

I

K

D

E

H

F

S

T

R

V

E

D

E

E

L

I

T

C

E

Q

A

A

T

T

L

I

E

D

L

L

I

V

E

R

R

A

Q

N

D

D

L

Y

E

R

S

G

-

D

C

V

Y

Y

I

I

R

M

P

P

I

L

A

A

F

Y

Y

A

G

V

Y

G

N

N

S

K

L

A

G

F

V

S

S

V

P

V

Q

G

D

D

C

R

P

T

T

T

Q

E

T

M

A

F

I

I

A

Y

C

S

W

R

P

P

W

A

G

V

T

S

Y

R

L

L

G

E

D

E

E

D

A

F

L

-

E

-

N

-

G

S

V

L

D

H

V

A

M

C

V

Y

S

S

W

W

R

T

K

R

H

I

A

N

S

E

S

R

Q

V

I

L

P

P

T

P

N

R

A

I

K
|
K

T

A

T

L

G

A

L

N

Y
|
Y

V

R

N

N

S

S

M

Q

L

L

A

A

G

S

E

S

E

E

A

A

R

A

R

M

N

N

G

G

Y

Y

V

D

E

T

A

A

I

L

V

F

L

L

N

N

K

P

E

E

G

G

N

K

V

V

A

A

E
|
E

G

G

P

T

G
|
G

E
x
S

N
x
C

L

V

F

F

L

F

V

V

R

R

D

K

G

G

T

K

I

L

H

I

T

T

P

P

G

D

F

I

A

T

E

S

S

G

I

I

L
|
L

D

E

G
x
S

I
|
I

T
|
T

R

R

Q

D

S

T

V

V

V

I

T

H

L

L

A

A

R

R

E

E

-

V

R

L

G

G

Y

L

D

E

V

V

D

E

S

R

A

V

S

-

I

V

S

D

R

R

G

T

E

E

L

T

Y

Y

T

L

A

A

D

D

E

E

L

A

F

F

F

L

T

C

G
|
G

T
|
T

A

H

A

A

E

E

I

I

T

T

P

P

I

I

R

A

S

S

V

I

D

D

D

R

T

H

A

E

I

M

G

K

T

H

G

G

E

A

R

P

G

G

P

P

V

I

T

T

E

R

E

Q

L

L

Q

R

S

D

A

I

F

Y

F

R

D

E

L

V

V

V

E

Y

R

G

R

R

T

D

D

F

D

R

H

Y

D

R

E

N

W

W

F

L

T

T

active site: F34 (= F39), K156 (= K161), E190 (= E195), L214 (= L219)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R59 (= R62), K156 (= K161), Y161 (= Y166), E190 (= E195), G193 (= G198), S194 (≠ E199), C195 (≠ N200), L214 (= L219), S216 (≠ G221), I217 (= I222), T218 (= T223), G254 (= G259), T255 (= T260)

7neaA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum (m3 mutant). (see paper)
34% identity, 96% coverage: 11:307/309 of query aligns to 6:302/309 of 7neaA

query
sites
7neaA

V

L

W

W

M

W

D

N

G

H

E

R

F

I

V

I

D

P

W

W

D

E

E

E

A

A

Q

T

V

V

H

H

V

L

L

T

T

D

H

Y

G

W

L

W

H

A

Y

S

G

V

S

T

G

A

V

V

F
|
F

E

E

G

V

V

I

R

S

C

G

Y

Y

-

W

D

N

T

N

E

A

E

E

G

G

P

E

A

M

-

Y

I

I

F

F

R

R

W

L

D

E

E

D

H

H

L

A

D

R

R
|
R

F

L

Y

E

N

Q

S

S

A

M

K

Q

P

L

Y

I

D

R

M

M

E

P

I

K

D

E

H

F

S

T

R

V

E

D

E

E

L

I

T

C

E

Q

A

A

T

T

L

I

E

D

L

L

I

V

E

R

R

A

Q

N

D

D

L

Y

E

R

S

G

-

D

C

V

Y

F

I

I

R

M

P

P

I

L

A

A

F

Y

Y

A

G

V

Y

G

N

N

S

K

L

A

G

F

V

S

S

V

P

V

Q

G

D

D

C

R

P

T

T

T

Q

E

T

M

A

F

I

I

A

Y

C

S

W

R

P

P

W

A

G

V

T

S

Y

R

L

L

G

E

D

E

E

D

A

F

L

-

E

-

N

-

G

S

V

L

D

H

V

A

M

C

V

Y

S

S

W

W

R

T

K

R

H

I

A

N

S

E

S

R

Q

V

I

L

P

P

T

P

N

R

A

I

K
|
K

T

A

T

L

G

A

L

N

Y
|
Y

V

R

N

N

S

S

M

Q

L

L

A

A

G

S

E

S

E

E

A

A

R

A

R

M

N

N

G

G

Y

Y

V

D

E

T

A

A

I

L

V

F

L

L

N

N

K

P

E

E

G

G

N

K

V

V

A

A

E
|
E

G

G

P

T

G
|
G

E
x
S

N

C

L

V

F

F

L

F

V

V

R

R

D

K

G

G

T

K

I

L

H

I

T

T

P

P

G

D

F

I

A

T

E

S

S

G

I

I

L
|
L

D

E

G
x
S

I
|
I

T
|
T

R

R

Q

D

S

T

V

V

V

I

T

H

L

L

A

A

R

R

E

E

-

V

R

L

G

G

Y

L

D

E

V

V

D

E

S

R

A

V

S

-

I

V

S

D

R

R

G

T

E

E

L

T

Y

Y

T

L

A

A

D

D

E

E

L

A

F

F

F

L

T

C

G

G

T
|
T

A

H

A

A

E

E

I

I

T

T

P

P

I

I

R

A

S

S

V

I

D

D

D

R

T

H

A

E

I

M

G

K

T

H

G

G

E

A

R

P

G

G

P

P

V

I

T

T

E

R

E

Q

L

L

Q

R

S

D

A

I

F

Y

F

R

D

E

L

V

V

V

E

Y

R

G

R

R

T

D

D

F

D

R

H

Y

D

R

E

N

W

W

F

L

T

T

active site: F34 (= F39), K156 (= K161), E190 (= E195), L214 (= L219)
binding pyridoxal-5'-phosphate: R59 (= R62), K156 (= K161), Y161 (= Y166), E190 (= E195), G193 (= G198), S194 (≠ E199), L214 (= L219), S216 (≠ G221), I217 (= I222), T218 (= T223), T255 (= T260)

7p3tB Transaminase of gamma-proteobacterium (see paper)
30% identity, 94% coverage: 7:296/309 of query aligns to 2:284/299 of 7p3tB

query
sites
7p3tB

D

D

V

E

D

P

T

I

V

I

W

Y

M

I

D

N

G

G

E

D

F

Y

V

L

D

P

W

L

D

S

E

Q

A

A

Q

R

V

V

H

S

V

P

L

V

T

D

H

Q

G

G

L

F

H

L

Y

L

G

G

S

D

G

G

V

V

F

F

E

D

G

V

V

V

R

S

C

A

Y

W

D

K

T

G

E

N

E

-

G

-

P

-

A

-

I

I

F

F

R

K

W

L

D

D

E

A

H

H

L

L

D

D

R
|
R

F

F

Y

F

N

D

S

S

A

I

K

Q

P

A

Y

A

D

R

M

L

E

N

I

H

D

D

H

M

S

S

R

R

E

D

E

A

L

W

T

K

E

E

A

A

T

I

L

I

E

E

L

T

I

T

E

R

R

R

Q

N

D

G

L

L

E

D

S

D

C

A

Y

S

I

I

R

R

P

F

I

I

A

V

F

T

Y

R

G

G

Y

E

N

P

S

K

L

G

G

V

V

V

S

A

-

D

P

P

Q

R

D

D

C

F

P

K

T

P

Q

T

T

C

A

I

I

V

A

W

C

V

W

A

P

P

W

Y

G

I

T

F

Y

L

L

A

G

D

D

E

E

E

A

K

L

R

E

R

N

N

G

G

V

I

D

R

V

L

M

M

V

I

S

S

S

A

W

T

R

R

K

G

H

F

A

P

S

A

S

D

Q

T

I

L

P

D

T

P

N

R

A

Y

K
|
K

T

C

T

L

G

D

L

-

Y
x
R

V

L

N

H

S

S

M

Q

L

L

A

I

G

R

E

L

E

E

A

A

R

L

R

E

N

A

G

G

Y

Y

V

D

E

D

A

A

I

L

V

W

L

L

N

D

K

H

E

S

G

G

N

H

V

V

A

S

E
|
E

G
x
S

P
x
A

G
x
A

E
x
S

N

N

L

L

F

F

L

I

V

V

R

K

D

N

G

G

T

V

I

L

H

Y

T

T

P

P

G

-

F

-

A

S

E

A

S

G

I

I

L

L

D

R

G
|
G

I
|
I

T
|
T

R

R

Q

D

S

T

V

I

V

L

T

E

L

L

A

A

R

T

E

E

R

-

G

-

Y

L

D

D

V

I

D

P

-

W

D

K

S

E

A

R

S

Q

I

L

S

S

R

A

G

F

E

D

L

V

Y

Y

T

I

A

A

D

D

E

E

L

V

F

F

F

T

T

C

G

S

T
|
T

A

A

A

G

E

G

I

A

T

L

P

P

I

V

R

R

S

E

V

V

D

A

D

G

T

R

A

T

I

I

G

R

T

G

G

T

E

T

R

P

G

G

P

P

V

I

T

T

E

Q

E

A

L

I

Q

D

S

N

A

A

F

Y

F

W

D

A

L

M

V

R

E

E

binding pyridoxal-5'-phosphate: R53 (= R62), K153 (= K161), R157 (≠ Y166), E186 (= E195), S187 (≠ G196), A188 (≠ P197), A189 (≠ G198), S190 (≠ E199), G210 (= G221), I211 (= I222), T212 (= T223), T248 (= T260)

6xu3A (R)-selective amine transaminase from shinella sp. (see paper)
26% identity, 91% coverage: 12:293/309 of query aligns to 29:304/320 of 6xu3A

query
sites
6xu3A

W

Y

M

I

D

A

G

G

E

E

F

Y

V

V

D

P

W

L

D

H

E

E

A

A

Q

R

V

I

H

P

V

I

L

L

T

D

H

Q

G

G

L

F

H

L

Y

H

G

S

S

D

G

L

V

T

F
x
Y

E

D

G

V

V

P

R

A

C

V

Y

W

D

N

T

G

E

R

E

-

G

-

P

-

A

-

I

F

F

F

R

R

W

L

D

E

E

D

H

H

L

L

D

D

R
|
R

F

F

Y

E

N

K

S

S

A

C

K

A

P

Q

Y

L

D

R

M

L

E

K

I

S

D

P

H

L

S

S

R

R

E

E

L

I

T

R

E

D

A

R

T

L

L

V

E

E

L

M

I

T

E

V

R

K

Q

S

D

G

L

I

E

R

S

D

C

A

Y

Y

I

V

R

M

P

M

I

I

A

V

F

T

Y

R

G

G

Y

L

N

R

S

F

L

V

-

R

G

Q

V

Y

S

A

P

P

Q

E

D

E

C

C

P

D

T

N

Q

F

T

C

A

Y

I

L

A

M

C

V

W

M

P

P

W

Y

G

-

T

L

Y

W

L

V

G

M

D

D

E

E

A

A

L

T

E

Q

N

K

-

N

-

G

G

G

V

S

D

A

V

V

M

I

V

T

S

R

S

T

W

V

R

R

K

R

H

V

A

P

S

P

S

G

Q

A

I

I

P

D

T

P

N

T

A

V

K
|
K

T

N

T

L

-

Q

-

W

G

G

L

D

Y
x
F

V

T

N

R

S

G

M

L

L

M

A

-

G

-

E

-

E

E

A

A

R

R

R

D

N

R

G

G

Y

A

V

M

E

Y

A

P

I

I

V

L

L

T

N

D

K

G

E

D

G

A

N

N

V

L

A

T

E
|
E

G

G

P

S

G
|
G

E
x
F

N

N

L

V

F

I

L

L

V

I

R

K

D

D

G

G

T

K

I

L

H

Y

T

T

P

P

G

-

F

-

A

R

E

K

S

G

I

V

L
|
L

D

E

G
|
G

I
x
V

T
|
T

R

R

Q

K

S

S

V

V

V

L

T

A

L

V

A

A

R

E

E

K

R

L

G

G

Y

Y

D

P

-

Y

-

T

V

I

D

D

S

-

A

-

S

-

I

V

S

P

R

V

G

E

E

L

L

A

Y

Y

T

Q

A

C

D

D

E

E

L

I

F

L

F

F

T

V

G

T

T
|
T

A

A

A

G

E

G

I

V

T

M

P

P

I

I

R

T

S

T

V

L

D

D

D

G

T

Q

A

P

I

V

G

G

T

D

G

G

E

Q

R

V

G

G

P

P

V

I

T

S

E

K

E

A

L

L

Q

W

S

K

A

G

F

Y

F

W

D

D

active site: Y56 (≠ F39), K176 (= K161), E209 (= E195), L231 (= L219)
binding pyridoxal-5'-phosphate: R75 (= R62), K176 (= K161), F183 (≠ Y166), E209 (= E195), G212 (= G198), F213 (≠ E199), L231 (= L219), G233 (= G221), V234 (≠ I222), T235 (= T223), T271 (= T260)

6xu3C (R)-selective amine transaminase from shinella sp. (see paper)
26% identity, 91% coverage: 12:293/309 of query aligns to 31:306/322 of 6xu3C

query
sites
6xu3C

W

Y

M

I

D

A

G

G

E

E

F

Y

V

V

D

P

W

L

D

H

E

E

A

A

Q

R

V

I

H

P

V

I

L

L

T

D

H

Q

G

G

L

F

H

L

Y

H

G

S

S

D

G

L

V

T

F
x
Y

E

D

G
x
V

V

P

R

A

C

V

Y

W

D

N

T

G

E

R

E

-

G

-

P

-

A

-

I

F

F

F

R

R

W

L

D

E

E

D

H

H

L

L

D

D

R
|
R

F

F

Y

E

N

K

S

S

A

C

K

A

P

Q

Y

L

D

R

M

L

E

K

I

S

D

P

H

L

S

S

R

R

E

E

L

I

T

R

E

D

A

R

T

L

L

V

E

E

L

M

I

T

E

V

R

K

Q

S

D

G

L

I

E

R

S

D

C

A

Y

Y

I

V

R

M

P

M

I

I

A

V

F

T

Y

R

G

G

Y

L

N

R

S

F

L

V

-

R

G

Q

V

Y

S

A

P

P

Q

E

D

E

C

C

P

D

T

N

Q

F

T

C

A

Y

I

L

A

M

C

V

W

M

P

P

W

Y

G

-

T

L

Y

W

L

V

G

M

D

D

E

E

A

A

L

T

E

Q

N

K

-

N

-

G

G

G

V

S

D

A

V

V

M

I

V

T

S

R

S

T

W

V

R

R

K

R

H

V

A

P

S

P

S

G

Q

A

I

I

P

D

T

P

N

T

A

V

K
|
K

T

N

T

L

-

Q

-

W

G

G

L

D

Y
x
F

V

T

N

R

S

G

M

L

L

M

A

-

G

-

E

-

E

E

A

A

R

R

R

D

N

R

G

G

Y

A

V

M

E

Y

A

P

I

I

V

L

L

T

N

D

K

G

E

D

G

A

N

N

V

L

A

T

E
|
E

G

G

P

S

G
|
G

E

F

N
|
N

L

V

F

I

L

L

V

I

R

K

D

D

G

G

T

K

I

L

H

Y

T

T

P

P

G

-

F

-

A

R

E

K

S

G

I

V

L
|
L

D

E

G
|
G

I
x
V

T
|
T

R

R

Q

K

S

S

V

V

V

L

T

A

L

V

A

A

R

E

E

K

R

L

G

G

Y

Y

D

P

-

Y

-

T

V

I

D

D

S

-

A

-

S

-

I

V

S

P

R

V

G

E

E

L

L

A

Y

Y

T

Q

A

C

D

D

E

E

L

I

F

L

F

F

T

V

G
x
T

T
|
T

A
|
A

A

G

E

G

I

V

T

M

P

P

I

I

R

T

S

T

V

L

D

D

D

G

T

Q

A

P

I

V

G

G

T

D

G

G

E

Q

R

V

G

G

P

P

V

I

T

S

E

K

E

A

L

L

Q

W

S

K

A

G

F

Y

F

W

D

D

active site: Y58 (≠ F39), K178 (= K161), E211 (= E195), L233 (= L219)
binding n-glycine-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-yl-methane]: V60 (≠ G41), R77 (= R62), K178 (= K161), F185 (≠ Y166), E211 (= E195), G214 (= G198), N216 (= N200), L233 (= L219), G235 (= G221), V236 (≠ I222), T237 (= T223), T272 (≠ G259), T273 (= T260), A274 (= A261)

6xu3B (R)-selective amine transaminase from shinella sp. (see paper)
26% identity, 91% coverage: 12:293/309 of query aligns to 30:305/321 of 6xu3B

query
sites
6xu3B

W

Y

M

I

D

A

G

G

E

E

F

Y

V

V

D

P

W

L

D

H

E

E

A

A

Q

R

V

I

H

P

V

I

L

L

T

D

H

Q

G

G

L

F

H

L

Y

H

G

S

S

D

G

L

V

T

F
x
Y

E

D

G

V

V

P

R

A

C

V

Y

W

D

N

T

G

E

R

E

-

G

-

P

-

A

-

I

F

F

F

R

R

W

L

D

E

E

D

H

H

L

L

D

D

R
|
R

F

F

Y

E

N

K

S

S

A

C

K

A

P

Q

Y

L

D

R

M

L

E

K

I

S

D

P

H

L

S

S

R

R

E

E

L

I

T

R

E

D

A

R

T

L

L

V

E

E

L

M

I

T

E

V

R

K

Q

S

D

G

L

I

E

R

S

D

C

A

Y
|
Y

I

V

R

M

P

M

I

I

A

V

F

T

Y

R

G

G

Y

L

N

R

S

F

L

V

-

R

G

Q

V

Y

S

A

P

P

Q

E

D

E

C

C

P

D

T

N

Q

F

T

C

A

Y

I

L

A

M

C

V

W

M

P

P

W

Y

G

-

T

L

Y

W

L

V

G

M

D

D

E

E

A

A

L

T

E

Q

N

K

-

N

-

G

G

G

V

S

D

A

V

V

M

I

V

T

S

R

S

T

W

V

R

R

K

R

H

V

A

P

S
x
P

S

G

Q

A

I

I

P
x
D

T

P

N

T

A

V

K
|
K

T

N

T

L

-

Q

-

W

G

G

L

D

Y
x
F

V

T

N

R

S

G

M

L

L

M

A

-

G

-

E

-

E

E

A

A

R

R

R

D

N

R

G

G

Y

A

V

M

E

Y

A

P

I

I

V

L

L

T

N

D

K

G

E

D

G

A

N

N

V

L

A

T

E
|
E

G

G

P

S

G
|
G

E
x
F

N
|
N

L

V

F

I

L

L

V

I

R

K

D

D

G

G

T

K

I

L

H

Y

T

T

P

P

G

-

F

-

A

R

E
x
K

S

G

I

V

L
|
L

D

E

G
|
G

I
x
V

T
|
T

R

R

Q

K

S

S

V

V

V

L

T

A

L

V

A

A

R

E

E

K

R

L

G

G

Y

Y

D

P

-

Y

-

T

V

I

D

D

S

-

A

-

S

-

I

V

S

P

R

V

G

E

E

L

L

A

Y

Y

T

Q

A

C

D

D

E

E

L

I

F

L

F

F

T

V

G

T

T
|
T

A

A

A

G

E

G

I

V

T

M

P

P

I

I

R

T

S

T

V

L

D

D

D

G

T

Q

A

P

I

V

G

G

T

D

G

G

E

Q

R

V

G

G

P

P

V

I

T

S

E

K

E

A

L

L

Q

W

S

K

A

G

F

Y

F

W

D

D

active site: Y57 (≠ F39), K177 (= K161), E210 (= E195), L232 (= L219)
binding 3-aminobenzoic acid: P169 (≠ S153), D173 (≠ P157), K229 (≠ E216)
binding n-glycine-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-yl-methane]: R76 (= R62), Y112 (= Y98), K177 (= K161), F184 (≠ Y166), E210 (= E195), G213 (= G198), F214 (≠ E199), N215 (= N200), L232 (= L219), G234 (= G221), V235 (≠ I222), T236 (= T223), T272 (= T260)

Query Sequence

>WP_007695195.1 NCBI__GCF_000336675.1:WP_007695195.1
MGFDEMDVDTVWMDGEFVDWDEAQVHVLTHGLHYGSGVFEGVRCYDTEEGPAIFRWDEHL
DRFYNSAKPYDMEIDHSREELTEATLELIERQDLESCYIRPIAFYGYNSLGVSPQDCPTQ
TAIACWPWGTYLGDEALENGVDVMVSSWRKHASSQIPTNAKTTGLYVNSMLAGEEARRNG
YVEAIVLNKEGNVAEGPGENLFLVRDGTIHTPGFAESILDGITRQSVVTLARERGYDVDD
SASISRGELYTADELFFTGTAAEITPIRSVDDTAIGTGERGPVTEELQSAFFDLVERRTD
DHDEWFTHL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory