SitesBLAST

Q58487 Mevalonate kinase; MK; MVK; EC 2.7.1.36 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
31% identity, 89% coverage: 3:295/329 of query aligns to 2:285/312 of Q58487

query
sites
Q58487

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R196 (≠ G198) mutation to K: 13.5-fold decrease in affinity for mevalonate, whereas only little effect on affinity for ATP and on reaction rate.; mutation to Q: 1900-fold decrease in catalytic efficiency.; mutation to V: 63-fold decrease in catalytic efficiency.
K272 (= K282) mutation K->R,A: 13- to 26-fold decrease in catalytic efficiency. Still thermostable.

2hfuA Crystal structure of l. Major mevalonate kinase in complex with r- mevalonate (see paper)
29% identity, 93% coverage: 8:314/329 of query aligns to 17:313/326 of 2hfuA

query
sites
2hfuA

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binding (r)-mevalonate: E24 (= E15), H25 (= H16), V27 (= V18), Y167 (≠ V176)

2dejA Crystal structure of galaktokinase from pyrococcus horikoshii with amp-pn and galactose
28% identity, 92% coverage: 1:303/329 of query aligns to 1:316/346 of 2dejA

query
sites
2dejA

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binding {5'-o-[(r)-{[(s)-amino(hydroxy-kappao)phosphoryl]oxy}(hydroxy-kappao)phosphoryl]adenosinato(2-)}magnesium: S47 (≠ L54), F50 (= F57), W67 (≠ V77), Y70 (= Y84), V92 (= V107), L98 (= L113), G99 (= G114), G101 (= G116), S103 (≠ G118), S104 (= S119), S105 (= S120), F108 (≠ V123)
binding alpha-D-galactopyranose: E15 (= E15), H16 (= H16), D18 (vs. gap), G146 (≠ S161), D149 (= D164), Y194 (vs. gap)

2deiA Crystal structure of galaktokinase from pyrococcus horikoshii with amp-pnp and galactose
28% identity, 92% coverage: 1:303/329 of query aligns to 1:320/350 of 2deiA

query
sites
2deiA

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L

L

L

A

S

K

A

N

L

Y

G

E

V

V

S

S

A

C

R

K

S

E

L

L

D

D

A

F

M

F

V

V

W

E

A

R

A

A

R

L

E

K

A

L

G

G

A

A

R

Y

G

G

A

A

K

R

L

L

T

T

G

G

S

A

G

G

G

F

G

G

C

S

I

A

V

I

A

A

L

L

D

V

E

D

T

K

E

E

D

D

T

A

P

E

T

T

binding alpha-D-galactopyranose: E15 (= E15), H16 (= H16), D18 (vs. gap), G146 (≠ S161), D149 (= D164), Y198 (≠ R210)
binding magnesium-5'-adenyly-imido-triphosphate: S47 (≠ L54), F50 (= F57), W67 (≠ V77), Y70 (= Y84), L98 (= L113), G99 (= G114), G101 (= G116), S103 (≠ G118), S104 (= S119), S105 (= S120), F108 (≠ V123)

P0A6T3 Galactokinase; Galactose kinase; EC 2.7.1.6 from Escherichia coli (strain K12) (see 2 papers)
27% identity, 91% coverage: 5:302/329 of query aligns to 24:350/382 of P0A6T3

query
sites
P0A6T3

S

Q

A

A

P

P

G

G

K

R

V

V

Y

N

L

L

F

I

G

G

E

E

H

H

A

T

V

D

V

Y

Y

N

G

D

E

G

P

F

A

V

V

L

P

P

C

C

A

A

I

I

E

D

R

Y

R

Q

A

T

R

V

V

I

E

S

A

C

T

A

Q

P

R

R

S

D

D

D

S

R

R

K

V

V

R

R

V

V

H

M

A

A

Q

A

D

D

L

Y

-

E

-

N

Y

Q

L

L

N

D

G

E

F

F

S

S

V

L

E

-

Y

-

A

-

G

-

P

-

A

-

D

-

D

D

R

A

P

P

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I

V

V

A

A

T

H

D

E

Q

N

S

Y

V

Q

L

W

D

-

L

-

G

-

M

A

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Y

Y

I

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D

R

G

G

A

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V

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K

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H

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D

-

L

L

A

R

D

N

Q

N

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E

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G

G

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D

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T

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P

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A

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S

A

A

A

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V

V

A

A

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I

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A

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T

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Y

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H

L

L

E

P

L

L

D

D

S

G

E

A

E

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I

A

A

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L

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N

A

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Y

Q

K

E

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A

E

E

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E

Q

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Q

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E

N

A

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I

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M

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D

T

Q

F

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C

I

S

S

A

A

T

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G

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A

K

A

D

K

H

V

A

E

L

G

L

D

I

S

D

C

C

E

R

T

S

I

L

-

G

-

T

D

K

A

A

P

V

N

S

L

M

P

P

-

K

-

G

-

V

-

A

-

V

-

I

-

N

-

S

-

N

-

F

-

K

-

R

F

T

V

L

V

V

G

G

Y

S

D

E

G

Y

G

N

T

T

-

R

-

E

-

Q

G

C

N

E

T

T

G

G

E

A

L

R

V

F

A

F

G

Q

V

Q

R

P

A

A

L

L

R

R

-

D

-

V

-

T

-

I

E

E

Y

F

D

N

F

A

A

V

A

A

S

H

T

E

V

L

E

D

S

P

I

I

G

-

D

-

L

V

V

A

R

K

R

R

G

V

E

R

H

H

-

I

-

L

-

T

-

E

-

N

-

A

-

R

-

T

-

V

-

E

-

A

-

S

A

A

L

L

D

E

A

Q

G

G

D

D

V

L

D

K

E

R

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M

G

G

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E

L

L

M

M

D

A

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E

N

S

H

H

G

A

-

S

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M

L

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S

D

A

D

L

F

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I

S

T

A

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S

Q

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I

D

D

A

T

M

L

V

V

W

E

A

I

A

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K

E

A

A

V

-

I

G

G

A

D

R

K

-

G

G

G

A

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K

R

L

M

T

T

G

G

S

G

G

G

G

F

G

G

C

C

I

I

V

V

A

A

L

L

D

I

E

P

T

E

E

E

D

L

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P

P

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
371 Y→H: Displays relaxed substrate specificity since it gains kinase activity toward sugars as diverse as D-galacturonic acid, D-talose, L-altrose, and L-glucose. Also shows enhanced turnover of the small pool of sugars converted by the wild-type enzyme.

2r42A The biochemical and structural basis for feedback inhibition of mevalonate kinase and isoprenoid metabolism (see paper)
31% identity, 81% coverage: 5:269/329 of query aligns to 9:306/383 of 2r42A

query
sites
2r42A

S

S

A

A

P

P

G

G

K

K

V

V

Y

I

L

L

F

H

G

G

E

E

H

H

A

A

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V

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V

A

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A

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S

D

N

S

G

R

K

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R

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N

A

L

Q

P

D
x
N

-

V

-

G

-

I

-

K

-

Q

-

V

-

W

-

D

-

V

-

A

-

T

-

L

-

Q

L

L

Y

L

L

D

N

T

G

G

F

F

S

Q

V

L

E

E

Y

K

A

L

G

K

P

K

A

V

D

A

D

G

R

L

P

P

-

R

-

D

T

C

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V

A

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T

N

D

E

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S

L

L

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L

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A

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F

M

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Y

Y

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Y

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A

A

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L

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S

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-

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G

-

L

L

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D

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L

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P

L

P

G
|
G

A

A

G
|
G

L

L

G
|
G

S
|
S

A
|
A

A

A

-
x
Y

-

S

V

V

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C

V

V

A

A

G

A

I

A

D

L

A

L

T

T

A

A

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-

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A

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I

N

H

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|
D

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F

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W

G

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A

A

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A

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A

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Y

N

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N

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E

A

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A

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A

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K

Y

F

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P

D

E

F

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A

M

A

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V

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S

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D

A

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C

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R

A

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L

L

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G

-

E

-

M

-

A

A

A

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A

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V

D

P

E

E

-

Q

-

Y

-

L

-

V

L

L

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E

R

E

L

L

M

M

D

D

V

M

N

N

H

Q

G

H

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H

L

L

S

N

A

A

L

L

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G

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V

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G

A

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R

A

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S

L

L

D

D

A

Q

M

L

binding s-[(2e,6e)-3,7,11-trimethyldodeca-2,6,10-trienyl] trihydrogen thiodiphosphate: N55 (≠ D51), G128 (= G114), G130 (= G116), G132 (= G118), S133 (= S119), S134 (= S120), Y137 (vs. gap)
binding magnesium ion: G132 (= G118), A135 (= A121), D192 (= D164)

Q03426 Mevalonate kinase; MK; EC 2.7.1.36 from Homo sapiens (Human) (see 12 papers)
29% identity, 85% coverage: 5:284/329 of query aligns to 9:332/396 of Q03426

query
sites
Q03426

S

S

A

A

P

P

G

G

K

K

V

V

Y

I

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L

F

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G

G

E
|
E

H
|
H

A

A

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Y

H

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K

P

V

A

A

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x
S

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x
I

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I

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L

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x
T

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Y

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A

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Q

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P

L

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G

G

A

A

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G

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L

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|
S

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x
Y

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C

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L

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A

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L

N

E

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S

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N

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K

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N

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x
E

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I

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H

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G

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|
S

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x
G

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D

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A

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G

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N

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|
T

G

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A

L

L

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V

A

A

G

G

V

V

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R

A

N

-

R

L

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P

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F

I

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A

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I
|
I

G

D

D

A

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C

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H

R

A

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L

L

D

-

A

-

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G

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M

D

G

E

E

-

A

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-

A

-

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-

E

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-

Y

-

L

-

V

L

L

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E

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x
E

L

L

M

I

D

D

V

M

N
|
N

H

Q

G

H

L

H

L

L

S

N

A

A

L

L

G

G

V
|
V

S

G

A

H

R

A

S

S

L

L

D

D

A

Q

M

L

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C

W

Q

A

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A

A

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G

G

A

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G

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S

K

K

L

L

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T

E19 (= E15) mutation to D: No change in protein stability. Weak decrease in kinase activity. Approximately 2-fold decrease in Vmax. Approximately 2-fold decrease affinity for ATP and mevalonate.
H20 (= H16) to P: in HIDS and MEVA; dbSNP:rs104895295
S52 (vs. gap) to N: in dbSNP:rs7957619
I56 (vs. gap) mutation to A: No effect on kinase activity. Approximately 4- and 5-fold decrease affinities for ATP and mevalonate, respectively.
T104 (≠ D74) mutation to A: No effect on kinase activity. Approximately 4-fold increase affinity for ATP. Normal affinity for mevalonate.
S145 (= S119) mutation to A: Modest changes in KM for ATP. 20-fold increase in KM for mevalonate. Approximately 2-fold decrease in Vmax.
S146 (= S120) mutation to A: Modest changes in KM for ATP. 20-fold increase in KM for mevalonate. 4000-fold decrease in Vmax.
Y149 (≠ V123) mutation to A: No effect on kinase activity. Approximately 4- and 8-fold decrease affinities for ATP and mevalonate, respectively.
E193 (vs. gap) mutation to Q: No change in protein stability. Decreased kinase activity. Approximately 50-fold decrease in Vmax. Approximately 20- and 40-fold decrease affinities for ATP and mevalonate, respectively.
I196 (vs. gap) mutation to A: No effect on kinase activity. Approximately 2- and 3-fold decrease affinities for ATP and mevalonate, respectively.
S201 (= S161) mutation to A: Modest changes in KM for ATP. 100-fold increase in KM for mevalonate. Approximately 2-fold increase in Vmax.
G202 (≠ R162) to R: in HIDS and POROK3; dbSNP:rs104895301
D204 (= D164) mutation to A: No change in protein stability. Loss of kinase activity. Normal affinities for ATP and mevalonate.; mutation to N: No change in protein stability. Loss of kinase activity. Normal affinities for ATP and mevalonate.
T243 (= T202) mutation to A: Modest changes in KM for ATP. 40-fold increase in KM for mevalonate. Approximately 2-fold decrease in Vmax.
I268 (= I226) to T: in HIDS and MEVA; dbSNP:rs104895304
E296 (≠ R247) mutation to Q: No change in protein stability. No effect on kinase activity.
N301 (= N252) to T: in MEVA; diminished activity; dbSNP:rs121917789
V310 (= V261) to M: in MEVA and HIDS; dbSNP:rs104895319

Sites not aligning to the query:

356 T → M: in dbSNP:rs104895342
377 V → I: in HIDS; most frequent mutation; dbSNP:rs28934897

1kvkA The structure of binary complex between a mammalian mevalonate kinase and atp: insights into the reaction mechanism and human inherited disease (see paper)
30% identity, 81% coverage: 5:269/329 of query aligns to 9:302/378 of 1kvkA

query
sites
1kvkA

S

S

A

A

P

P

G

G

K

K

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V

Y

I

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L

F

H

G

G

E

E

H

H

A

A

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Y

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K

P

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A

A

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A

A

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A

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N

S

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N

A

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D

N

L

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G

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K

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W

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-

A

Y

T

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N

Q

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V

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E

E

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K

A

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K

P

K

A

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D

A

D

G

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L

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P

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R

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D

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C

V

V

A

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T
x
N

D
x
E

Q

G

S

L

V
x
S

L

L

D

L

L

A

G

F

M

L

R

Y

Y

L

I

Y

D

L

G

A

A

I

I

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D

R

R

K

A

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R

R

D

T

L

L

A

P

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S

Q

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P

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G

-

L

L

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L

L

P

P

L

P

G
|
G

A

A

G
|
G

L

L

G
|
G

S
|
S

A
|
A

A

A

-

Y

-

S

V

V

V

C

V

V

A

A

G

A

I

A

D

L

A

L

T

T

A

A

R

C

E

E

-

E

-

V

-

T

-

N

-

P

-

L

-

K

-

D

-

R

-

G

-

S

L

I

G

G

-

S

-

W

L

P

E

E

L

E

D

D

S

L

E

K

E

S

V

I

A

N

N

K

H

W

A

A

Y

Y

K

E

V

G

E
|
E

H

-

E

R

V

V

Q
x
I

N
x
H

G

G

E

N

A

P

S

S

R

G

A

V

D
|
D

T

N

F

S

C

V

S

S

A

T

T

W

G

G

A

A

A

L

K

R

V

Y

E

Q

G

Q

D

G

S

K

C

M

E

S

T

S

I

L

D

K

-

R

A

L

P

P

N

A

L

L

P

Q

F

I

V

L

G

T

Y

N

D

T

G

K

G

V

T

P

G

R

N

S

T

T

G

K

E

A

L

L

V

V

A

A

G

G

V

V

R

R

A

S

L

R

R

L

E

I

E

K

Y

F

-

P

D

E

F

I

A

M

A

A

S

P

T

L

V

L

E

T

S

S

I

I

G

D

D

A

L

I

V

S

R

L

R

E

G

C

E

E

H

R

A

V

L

L

D

G

-

E

-

M

-

A

A

A

G

A

D

P

V

V

D

P

E

E

-

Q

-

Y

-

L

-

V

L

L

G

E

R

E

L

L

M

M

D

D

V

M

N

N

H

Q

G

H

L

H

L

L

S

N

A

A

L

L

G

G

V

V

S

G

A

H

R

A

S

S

L

L

D

D

A

Q

M

L

binding adenosine-5'-triphosphate: N88 (≠ T73), E89 (≠ D74), S92 (≠ V77), G124 (= G114), G126 (= G116), G128 (= G118), S129 (= S119), S130 (= S120), I180 (≠ Q156)
binding magnesium ion: G128 (= G118), S130 (= S120), A131 (= A121), E177 (= E152), H181 (≠ N157), D188 (= D164)

8tfoA Structure of mkvar (see paper)
29% identity, 89% coverage: 5:296/329 of query aligns to 10:349/390 of 8tfoA

query
sites
8tfoA

S

S

A

A

P

P

G

G

K

K

V

I

Y

I

L

L

F

H

G

G

E
|
E

H
|
H

A

A

V
|
V

V

V

Y

Y

G

Q

E

K

P

T

A

A

V

V

P

A

C

L

A

S

I

L

E

G

R

L

R

R

A

T

R

R

V

L

E

D

A

L

T

T

Q

E

R

T

S

T

D

D

S

G

R

R

V

I

R

S

V

I

H

-

A

I

Q

M

D

D

L

K

Y

F

L

L

N

Q

-

H

-

T

G

S

F

W

S

S

V

V

E

E

Y

E

A

L

G

S

P

K

A

I

D

I

D

D

R

K

-

V

-

K

-

I

-

D

-

A

-

N

P

P

T

E

V

T

A

E

T

L

D

D

Q

Q

S

E

V

L

L

V

D

E

-

D

-

L

-

R

-

M

-

T

-

S

-

G

-

H

-

T

-

Q

-

S

-

V

-

A

-

L

L

V

G

G

M

F

R

L

Y

Y

I

I

D

-

G

-

A

L

I

V

D

K

R

L

A

C

R

K

D

F

L

S

A

G

D

K

Q

Q

P

R

E

P

V

P

G

S

L

I

D

Q

V

I

T

S

V

I

E

S

S

S

D

D

L

I

P

A

L

I

G

S

A

A

G

G

L

L

G

G

S

S

A

A

V

F

V

A

V

V

A

C

G

L

I

S

D

A

A

S

T

L

A

L

R

S

E

Y

L

L

G

G

L

I

E

I

L

V

-

C

-

D

-

R

-

K

-

N

-

C

-

A

-

D

-

V

-

D

-

G

-

K

-

L

-

V

-

P

-

S

-

A

D

D

S

Q

E

L

E

A

V

L

A

I

N

N

H

H

-

W

A

A

Y

F

K

M

V

V

E

E

H

-

E

K

V

I

Q

V

N

H

G

G

E

S

A
|
A

S
|
S

R

G

A

V

D
|
D

T

N

F

A

C

V

S

S

A

T

T

Y

G

G

A

S

A

I

K

K

V

Y

E

R

G

N

D

N

S

E

C

L

E

T

T

R

I

I

-

G

D

S

A

G

P

L

N

K

L

L

P

D

F

V

V

L

V

I

G

V

Y

D

D

T

G

H

G

V

T

Q

G

R

N

D

T

T

G

K

E

K

L

M

V

L

A

D

G

I

V

V

R

R

A

H

L

R

R

R

E

K

E

L

Y

Y

D

P

F

A

A

I

A

T

S

N

T

P

V

V

-

L

E

E

S

A

I

I

G

D

D

G

L

I

V

S

R

E

R

T

G

S

E

S

H

K

A

I

L

L

D

Q

A

H

G

G

D

D

-

G

-

L

-

P

-

T

-

G

-

E

V

Y

D

E

E

V

L

I

G

A

R

D

L

L

M

V

D

R

V

M

N

N

H

Q

G

N

L

L

S

S

A

T

L

L

G

G

V

V

S

S

A

H

R

P

S

K

L

L

D

D

A

V

M

I

V

C

W

E

A

T

A

A

R

S

E

R

A

F

G

G

A

Q

R

A

G

G

A

-

K

K

L

L

T

T

G

G

S

A

G

G

C

C

-

A

I

I

V

V

A

V

L

L

D

D

binding (r)-mevalonate: E20 (= E15), H21 (= H16), V23 (= V18), A204 (= A160), S205 (= S161), D208 (= D164)

P17256 Mevalonate kinase; MK; EC 2.7.1.36 from Rattus norvegicus (Rat) (see 2 papers)
29% identity, 81% coverage: 5:269/329 of query aligns to 9:318/395 of P17256

query
sites
P17256

S

S

A

A

P

P

G

G

K
|
K

V

V

Y

I

L

L

F

H

G

G

E

E

H
|
H

A

A

V

V

Y

H

G

G

E

K

P

V

A

A

V

L

P

A

C

V

A

A

I

L

E

N

R

L

R

R

A

T

R

F

V

L

E

V

A

L

T

R

Q

P

R

Q

S

S

D

N

S

G

R

K

V

V

R

S

V

L

H

N

A

L

Q

P

D
x
N

L

V

Y

G

L

I

N

K

-

Q

-

V

-

W

-

D

-

V

-

A

-

T

-

L

-

Q

-

L

-

D

-

T

G

G

F

F

S

L

V

E

E

Q

Y

G

A

D

G

V

P

P

A

A

D

P

D

T

R

L

P

E

T

Q

V

L

A

E

T

K

D

L

Q

K

S

K

V

V

L

A

D

G

L

L

G

P

M

R

R

D

Y

C

I

V

D

G

G
x
N

-

E

-

G

-

L

-
x
S

-

L

-

A

-

F

-

L

-

Y

-

L

-

Y

-

L

-

A

A

I

I

C

D

R

R

K

A

Q

R

R

D

T

L

L

A

P

D

S

Q

-

P

-

E

-

V

-

G

-

L

L

D

D

V

I

T

M

V

V

E

W

S
|
S

D

E

L

L

P

P

L

P

G
|
G

A
|
A

G
|
G

L
|
L

G
|
G

S
|
S

A

A

-

Y

-

S

V

V

V

C

V

V

A

A

G

A

I

A

D

L

A

L

T

T

A

A

R

C

E

E

-

E

-

V

-

T

-

N

-

P

-

L

-

K

-

D

-

R

-

G

-

S

L

I

G

G

-

S

-

W

L

P

E

E

L

E

D

D

S

L

E

K

E

S

V

I

A

N

N

K

H

W

A

A

Y

Y

K

E

V

G

E
|
E

H

-

E

R

V

V

Q

I

N

H

G

G

E

N

A

P

S

S

R

G

A

V

D

D

T

N

F

S

C

V

S

S

A

T

T

W

G

G

A

A

A

L

K

R

V

Y

E

Q

G

Q

D

G

S

K

C

M

E

S

T

S

I

L

D

K

-

R

A

L

P

P

N

A

L

L

P

Q

F

I

V

L

G

T

Y

N

D

T

G

K

G

V

T

P

G

R

N

S

T

T

G

K

E

A

L

L

V

V

A

A

G

G

V

V

R

R

A

S

L

R

R

L

E

I

E

K

Y

F

-

P

D

E

F

I

A

M

A

A

S

P

T

L

V

L

E

T

S

S

I

I

G

D

D

A

L

I

V

S

R

L

R

E

G

C

E

E

H

R

A

V

L

L

D

G

-

E

-

M

-

A

A

A

G

A

D

P

V

V

D

P

E

E

-

Q

-

Y

-

L

-

V

L

L

G

E

R

E

L

L

M

M

D

D

V

M

N

N

H

Q

G

H

L

H

L

L

S

N

A

A

L

L

G

G

V

V

S

G

A

H

R

A

S

S

L

L

D

D

A

Q

M

L

K13 (= K9) binding ATP
H20 (= H16) mutation to K: Induce significant secondary structural change.; mutation H->L,Y: Approximately 5-fold decrease in Vmax for (R,S)-mevalonate. Approximately 3-fold increase in KM for (R,S)-mevalonate.
N55 (≠ D51) binding ATP
N104 (≠ G87) binding ATP
S108 (vs. gap) binding ATP
S135 (= S109) binding ATP
140:146 (vs. 114:120, 100% identical) binding ATP
S146 (= S120) binding Mg(2+)
E193 (= E152) binding Mg(2+)

Q9HHB6 Galactokinase; Galactose kinase; EC 2.7.1.6 from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) (see paper)
26% identity, 91% coverage: 5:303/329 of query aligns to 7:322/352 of Q9HHB6

query
sites
Q9HHB6

S

K

A

S

P

P

G

G

K

R

V

V

Y

N

L

L

F

I

G

G

E
|
E

H
|
H

A
x
T

-
x
D

V

Y

V

T

Y

Y

G

G

E

Y

P

-

A

V

V

M

P

P

C

M

A

A

I

I

E

D

R

L

R

Y

A

T

R

I

V

I

E

T

A

A

T

E

Q

K

R

Y

S

D

D

K

S

V

R

Q

V

-

R

-

V

-

H

-

A

-

Q

-

D

-

L

L

Y

Y

L

S

N

E

G

H

F

F

S

N

V

E

E

E

Y

K

A

T

G

F

P

T

A

L

D

D

D

N

R

-

P

-

T

-

V

L

A

T

T

K

D

E

Q

G

S

S

V

W

L

I

D

D

L

-

G

-

M

-

R

-

Y

Y

I

V

D

K

G

G

A

V

I

L

D

W

R

V

A

L

R

-

D

-

L

I

A

Q

D

E

Q

G

P

Y

E

K

V

I

G

G

-

G

L

L

D

K

V

G

T

K

V

I

E

T

S

G

D

D

L

L

P

P

L

L

G

G

A

A

G

G

L

L

G

S

S

S

S
|
S

A

A

A

S

V

F

V

E

V

V

A

G

G

I

I

L

D

E

A

V

T

L

A

N

R

Q

E

L

L

Y

G

N

L

L

E

N

L

I

D

D

S

P

E

L

E

K

V

K

A

A

N

L

H

L

A

A

Y

K

K

K

V

A

E
|
E

H

N

E

E

V

F

Q

V

N

G

G

V

E

P

A

C

S

G

R

I

A

L

D

D

T

Q

F

F

C

A

S

V

A

V

T

F

G

G

G

K

A

K

A

D

K

N

V

V

E

I

G

F

D

L

S

D

C

T

E

Q

T

T

I

L

D

Q

A

Y

P

E

N

Y

L

I

P

P

F

F

-

P

-

K

-

D

-

V

-

S

-

V

V

L

V

V

G

F

Y

Y

D

-

G

-

T

T

G

G

N

V

T

K

G

R

E

E

L

L

V

A

A

S

G

S

V

E

R
x
Y

A

A

L

E

R

R

E

K

E

R

Y

-

D

-

F

I

A

A

A

E

S

E

T

S

V

L

E

R

S

I

I

L

G

G

D

K

-

E

-

S

-

K

-

E

-

V

-

T

-

E

-

K

-

D

-

L

-

G

-

K

-

L

-

P

-

L

-

H

-

R

-

K

-

F

-

S

-

Y

L

I

V

V

R

R

R

E

G

N

E

A

H

R

-

V

-

L

-

E

-

V

-

R

-

D

A

A

L

L

D

K

A

E

G

G

D

D

V

I

D

E

E

K

L

V

G

G

R

K

L

I

M

L

D

T

V

T

N

A

H

H

G

W

-

D

L

L

L

A

S

E

A

N

L

Y

G

R

V

V

S

S

A

C

R

E

S

E

L

L

D

D

A

F

M

F

V

V

W

K

A

K

A

A

R

M

E

E

A

L

G

G

A

A

R

Y

G

G

A

A

K

R

L

L

T

T

G

G

S

A

G

G

G

F

G

G

C

S

I

A

V

I

A

A

L

L

D

V

E

D

T

K

E

D

D

K

T

A

P

K

T

T

EHTD 17:20 (≠ EHA- 15:17) binding substrate
S107 (= S120) binding Mg(2+)
E139 (= E152) binding Mg(2+)
Y200 (≠ R210) binding substrate

1s4eF Pyrococcus furiosus galactokinase in complex with galactose, adp and magnesium (see paper)
26% identity, 91% coverage: 5:303/329 of query aligns to 6:321/351 of 1s4eF

query
sites
1s4eF

S

K

A

S

P

P

G

G

K

R

V

V

Y

N

L

L

F

I

G

G

E
|
E

H
|
H

A

T

-
x
D

V

Y

V

T

Y

Y

G

G

E

Y

P

-

A

V

V

M

P

P

C

M

A

A

I

I

E

D

R

L

R

Y

A

T

R

I

V

I

E

T

A

A

T

E

Q

K

R

Y

S

D

D

K

S

V

R

Q

V

-

R

-

V

-

H

-

A

-

Q

-

D

-

L

L

Y

Y

L
x
S

N

E

G

H

F
|
F

S

N

V

E

E

E

Y

K

A

T

G

F

P

T

A

L

D

D

D

N

R

-

P

-

T

-

V

L

A

T

T

K

D

E

Q

G

S

S

V
x
W

L

I

D

D

L

-

G

-

M

-

R

-

Y
|
Y

I

V

D

K

G

G

A

V

I

L

D

W

R

V

A

L

R

-

D

-

L

I

A

Q

D

E

Q

G

P

Y

E

K

V

I

G

G

-

G

L

L

D

K

V

G

T

K

V
x
I

E

T

S

G

D

D

L

L

P

P

L
|
L

G
|
G

A

A

G

G

L

L

G
x
S

S
|
S

A

A

A

S

V
x
F

V

E

V

V

A

G

G

I

I

L

D

E

A

V

T

L

A

N

R

Q

E

L

L

Y

G

N

L

L

E

N

L

I

D

D

S

P

E

L

E

K

V

K

A

A

N

L

H

L

A

A

Y

K

K

K

V

A

E

E

H

N

E

E

V

F

Q

V

N

G

G

V

E

P

A

C

S

G

R

I

A

L

D

D

T

Q

F

F

C

A

S

V

A

V

T

F

G

G

G

K

A

K

A

D

K

N

V

V

E

I

G

F

D

L

S

D

C

T

E

Q

T

T

I

L

D

Q

A

Y

P

E

N

Y

L

I

P

P

F

F

-

P

-

K

-

D

-

V

-

S

-

V

V

L

V

V

G

F

Y

Y

D

-

G

-

T

T

G

G

N

V

T

K

G

R

E

E

L

L

V

A

A

S

G

S

V

E

R
x
Y

A

A

L

E

R

R

E

K

E

R

Y

-

D

-

F

I

A

A

A

E

S

E

T

S

V

L

E

R

S

I

I

L

G

G

D

K

-

E

-

S

-

K

-

E

-

V

-

T

-

E

-

K

-

D

-

L

-

G

-

K

-

L

-

P

-

L

-

H

-

R

-

K

-

F

-

S

-

Y

L

I

V

V

R

R

R

E

G

N

E

A

H

R

-

V

-

L

-

E

-

V

-

R

-

D

A

A

L

L

D

K

A

E

G

G

D

D

V

I

D

E

E

K

L

V

G

G

R

K

L

I

M

L

D

T

V

T

N

A

H

H

G

W

-

D

L

L

L

A

S

E

A

N

L

Y

G

R

V

V

S

S

A

C

R

E

S

E

L

L

D

D

A

F

M

F

V

V

W

K

A

K

A

A

R

M

E

E

A

L

G

G

A

A

R

Y

G

G

A

A

K

R

L

L

T

T

G

G

S
x
A

G

G

G

F

G

G

C

S

I

A

V

I

A

A

L

L

D

V

E

D

T

K

E

D

D

K

T

A

P

K

T

T

binding adenosine-5'-diphosphate: S48 (≠ L54), F51 (= F57), W68 (≠ V77), Y71 (= Y84), I93 (≠ V107), L99 (= L113), G100 (= G114), S104 (≠ G118), S105 (= S119), S106 (= S120), F109 (≠ V123)
binding alpha-D-galactopyranose: E16 (= E15), H17 (= H16), D19 (vs. gap), Y199 (≠ R210), A304 (≠ S286)

6gr2A Structure of human galactokinase in complex with galactose and adp
27% identity, 93% coverage: 5:310/329 of query aligns to 30:361/383 of 6gr2A

query
sites
6gr2A

S

S

A

A

P

P

G

G

K

R

V

V

Y

N

L

L

F

I

G

G

E
|
E

H
|
H

A

T

V
x
D

V

Y

Y

N

G

Q

E

G

P

L

A

V

V

L

P

P

C

M

A

A

I

L

E

E

R

L

R

M

A

T

R

V

V

L

E

V

A

G

T

S

Q

P

R

R

S

K

D

D

S

G

R

L

V

V

R

-

V

-

H

-

A

-

Q

-

D

-

L

-

Y

-

L

-

N

-

G

-

F

-

S

S

V

L

E

L

Y
x
T

A

T

G
x
S

P

E

A

G

D

A

D

D

R

E

P

P

T

Q

-

R

-

L

-

Q

-

F

-

P

V

L

A

P

T

T

D

A

Q

Q

S

R

V

S

L

L

D

E

L

P

G

G

M

T

-

P

-

R

-

W

-

A

R

N

Y
|
Y

I

V

D

K

G

G

A

V

I

I

D

Q

R

Y

A

-

R

-

D

-

L

Y

A

P

D

A

Q

A

P

P

E

L

V

P

G

G

L

F

D

S

V

A

T

V

V

V

E

V

S

S

D

S

L

V

P

P

L
|
L

G
|
G

A

G

G
|
G

L

L

G
x
S

S
|
S

A

A

A

S

V

L

V

E

V

V

A

A

G

T

I

Y

D

T

A

F

T

L

A

Q

R

Q

E

-

L

-

G

-

L

L

E

C

L

P

D

D

S

S

E

G

E

T

V

I

A

A

N

A

H

R

A

A

Y

Q

-

V

-

C

-

Q

K

Q

V

A

E

E

H

H

E

S

V

F

Q

A

N

G

G

M

E

P

A

C

S
x
G

R

I

A

M

D
|
D

T

Q

F

F

C

I

S

S

A

L

T

M

G

G

G

Q

A

K

A

G

K

H

V

A

E

L

G

L

D

I

S

D

C

C

E

R

T

S

I

L

D

E

A

T

P

S

N

L

L

V

P

P

F

L

-

S

-

D

-

P

-

K

-

L

-

A

-

V

-

L

-

I

-

T

-

N

-

S

-

N

V

V

V

S

G

E

Y
|
Y

D

P

G

V

G

R

T

R

G

R

N

Q

T

C

G

E

E

E

L

V

V

A

-

R

-

A

A

L

G

G

V

A

R

A

A

S

L

L

R

R

E

E

E

-

Y

-

D

-

F

V

A

Q

A

L

S

E

T

E

V

L

E

E

S

A

I

A

G

R

D

D

L

L

V

V

-

S

-

K

-

E

-

G

-

F

R

R

G

A

E

R

H

H

-

V

-

G

-

E

-

I

-

R

-

T

-

A

-

Q

-

A

A

A

L

L

D

R

A

R

G

G

D

D

V

Y

D

R

E

A

L

F

G

G

R

R

L

L

M

M

D

V

V

E

N

S

H

H

-

R

G

S

L

L

L

R

S

D

A

D

L

Y

G

E

V

V

S

S

A

C

R

P

S

E

L

L

D

D

A

Q

M

L

V

V

W

E

A

A

R

L

E

A

A

V

-

P

G

G

A

V

R

Y

G

G

A

S

K

R

L

M

T

T

G

G

S

G

G

G

G

F

G

G

C

C

I

T

V

V

A

T

L

L

D

L

E

E

T

A

E

S

D

A

T

A

P

P

T

H

A

A

L

M

G

R

Y

H

T

I

P

Q

E

E

binding adenosine-5'-diphosphate: T74 (≠ Y61), S76 (≠ G63), Y106 (= Y84), L132 (= L113), G133 (= G114), G135 (= G116), S137 (≠ G118), S138 (= S119), S139 (= S120)
binding beta-D-galactopyranose: E40 (= E15), H41 (= H16), D43 (≠ V18), G180 (≠ S161), D183 (= D164), Y227 (= Y195)

1wuuA Crystal structure of human galactokinase complexed with mgamppnp and galactose (see paper)
27% identity, 93% coverage: 5:310/329 of query aligns to 34:369/391 of 1wuuA

query
sites
1wuuA

S

S

A

A

P

P

G

G

K

R

V

V

Y

N

L

L

F

I

G

G

E
|
E

H
|
H

A

T

V
x
D

V
x
Y

Y

N

G

Q

E

G

P

L

A

V

V

L

P

P

C

M

A

A

I

L

E

E

R

L

R

M

A

T

R

V

V

L

E

V

A

G

T

S

Q

P

R

R

S

K

D

D

S

G

R

L

V

V

R

-

V

-

H

-

A

-

Q

-

D

-

L

-

Y

-

L

-

N

-

G

-

F

-

S

S

V

L

E

L

Y
x
T

A

T

G
x
S

P

E

A

G

D

A

D

D

R

E

P

P

T

Q

-

R

-

L

-

Q

-

F

-

P

V

L

A

P

T

T

D

A

Q

Q

S

R

V

S

L

L

D

E

L

P

G

G

M

T

-

P

-

R

-
x
W

-

A

R

N

Y
|
Y

I

V

D

K

G

G

A

V

I

I

D

Q

R

Y

A

-

R

-

D

-

L

Y

A

P

D

A

Q

A

P

P

E

L

V

P

G

G

L

F

D

S

V

A

T

V

V

V

E

V

S

S

D

S

L

V

P

P

L
|
L

G
|
G

A

G

G
|
G

L

L

G
x
S

S
|
S

A

A

A

S

V
x
L

V

E

V

V

A

A

G

T

I

Y

D

T

A

F

T

L

A

Q

R

Q

E

-

L

-

G

-

L

L

E

C

L

P

D

D

S

S

E

G

E

T

V

I

A

A

N

A

H

R

A

A

Y

Q

-

V

-

C

-

Q

K

Q

V

A

E

E

H

H

E

S

V

F

Q

A

N

G

G

M

E

P

A

C

S
x
G

R

I

A
x
M

D
|
D

T

Q

F

F

C

I

S

S

A

L

T

M

G

G

G

Q

A

K

A

G

K

H

V

A

E

L

G

L

D

I

S

D

C

C

E

R

T

S

I

L

D

E

A

T

-

S

-

L

-

V

-

P

-

L

-

S

-

D

P

P

N

K

L

L

P

A

F

V

V

L

V

I

G

T

Y

N

D

S

G

N

G

V

T

R

G

H

N

A

T

S

G

S

E

E

L
x
Y

V

P

A

V

G

R

-

R

-

Q

-

C

-

E

-

V

V

A

R

R

A

A

L

L

R

G

E

K

E

E

-

S

-

L

Y

R

D

E

F

V

A

Q

A

L

S

E

T

E

V

L

E

E

S

A

I

A

G

R

D

D

L

L

V

V

-

S

-

K

-

E

-

G

-

F

R

R

G

A

E

R

H

H

-

V

-

G

-

E

-

I

-

R

-

T

-

A

-

Q

-

A

A

A

L

L

D

R

A

R

G

G

D

D

V

Y

D

R

E

A

L

F

G

G

R

R

L

L

M

M

D

V

V

E

N

S

H

H

-

R

G

S

L

L

L

R

S

D

A

D

L

Y

G

E

V

V

S

S

A

C

R

P

S

E

L

L

D

D

A

Q

M

L

V

V

W

E

A

A

R

L

E

A

A

V

-

P

G

G

A

V

R

Y

G

G

A

S

K

R

L

M

T

T

G

G

S

G

G

G

G

F

G

G

C

C

I

T

V

V

A

T

L

L

D

L

E

E

T

A

E

S

D

A

T

A

P

P

T

H

A

A

L

M

G

R

Y

H

T

I

P

Q

E

E

binding phosphoaminophosphonic acid-adenylate ester: T78 (≠ Y61), S80 (≠ G63), W107 (vs. gap), Y110 (= Y84), L136 (= L113), G137 (= G114), G139 (= G116), S141 (≠ G118), S142 (= S119), S143 (= S120), L146 (≠ V123)
binding alpha-D-galactopyranose: E44 (= E15), H45 (= H16), D47 (≠ V18), Y48 (≠ V19), G184 (≠ S161), M186 (≠ A163), D187 (= D164), Y235 (≠ L205)

6zfhA Structure of human galactokinase in complex with galactose and 2'- (benzo[d]oxazol-2-ylamino)-7',8'-dihydro-1'h-spiro[cyclopentane-1,4'- quinazolin]-5'(6'h)-one (see paper)
27% identity, 93% coverage: 5:310/329 of query aligns to 31:364/386 of 6zfhA

query
sites
6zfhA

S

S

A

A

P

P

G

G

K

R

V

V

Y

N

L

L

F

I

G

G

E
|
E

H
|
H

A

T

V
x
D

V

Y

Y

N

G

Q

E

G

P

L

A

V

V

L

P

P

C

M

A

A

I

L

E

E

R

L

R

M

A

T

R

V

V

L

E

V

A

G

T

S

Q

P

R

R

S

K

D

D

S

G

R

L

V

V

R

-

V

-

H

-

A

-

Q

-

D

-

L

-

Y

-

L

-

N

-

G

-

F

-

S

S

V

L

E

L

Y

T

A

T

G

S

P

E

A

G

D

A

D

D

R

E

P

P

T

Q

-

R

-

L

-

Q

-

F

-

P

V

L

A

P

T

T

D

A

Q

Q

S

R

V

S

L

L

D

E

L

P

G

G

M

T

-

P

-

R

-
x
W

-

A

R

N

Y
|
Y

I

V

D

K

G

G

A

V

I

I

D

Q

R

Y

A

-

R

-

D

-

L

Y

A

P

D

A

Q

A

P

P

E

L

V

P

G

G

L

F

D

S

V

A

T

V

V

V

E

V

S

S

D

S

L

V

P

P

L
|
L

G

G

A

G

G

G

L

L

G

S

S
|
S

A

A

A

S

V
x
L

V

E

V

V

A

A

G

T

I

Y

D

T

A

F

T

L

A

Q

R

Q

E

-

L

-

G

-

L

L

E

C

L

P

D

D

S

S

E

G

E

T

V

I

A

A

N

A

H

R

A

A

Y

Q

-

V

-

C

-

Q

K

Q

V

A

E

E

H

H

E

S

V

F

Q

A

N

G

G

M

E

P

A

C

S
x
G

R

I

A
x
M

D
|
D

T

Q

F

F

C

I

S

S

A

L

T

M

G

G

G

Q

A

K

A

G

K

H

V

A

E

L

G

L

D

I

S

D

C

C

E

R

T

S

I

L

D

E

A

T

P

S

N

L

L

V

P

P

F

L

-

S

-

D

-

P

-

K

-

L

V

A

V

V

G

L

Y

I

D

T

G

N

G

S

T

N

G

V

N

R

T

S

G

S

E

E

L
x
Y

-

P

-

V

-

R

-

Q

-

C

-

E

-

V

-

A

-

R

V

A

A

L

G

G

V

A

R

A

A

S

L

L

R

R

E

E

E

-

Y

-

D

-

F

V

A

Q

A

L

S

E

T

E

V

L

E

E

S

A

I

A

G

R

D

D

L

L

V

V

-

S

-

K

-

E

-

G

-

F

R

R

G

A

E

R

H

H

-

V

-

G

-

E

-

I

-

R

-

T

-

A

-

Q

-

A

A

A

L

L

D

R

A

R

G

G

D

D

V

Y

D

R

E

A

L

F

G

G

R

R

L

L

M

M

D

V

V

E

N

S

H

H

-

R

G

S

L

L

L

R

S

D

A

D

L

Y

G

E

V

V

S

S

A

C

R

P

S

E

L

L

D

D

A

Q

M

L

V

V

W

E

A

A

R

L

E

A

A

V

-

P

G

G

A

V

R

Y

G

G

A

S

K

R

L

M

T

T

G

G

S
x
G

G

G

G

F

G

G

C

C

I

T

V

V

A

T

L

L

D

L

E

E

T

A

E

S

D

A

T

A

P

P

T

H

A

A

L

M

G

R

Y

H

T

I

P

Q

E

E

binding beta-D-galactopyranose: E41 (= E15), H42 (= H16), D44 (≠ V18), G181 (≠ S161), M183 (≠ A163), D184 (= D164), Y230 (≠ L205), G340 (≠ S286)
binding 2-(1,3-benzoxazol-2-ylamino)spiro[1,6,7,8-tetrahydroquinazoline-4,1'-cyclopentane]-5-one: W104 (vs. gap), Y107 (= Y84), L133 (= L113), S139 (= S119), S140 (= S120), L143 (≠ V123)

6tepA Crystal structure of a galactokinase from bifidobacterium infantis in complex with adp (see paper)
27% identity, 89% coverage: 3:295/329 of query aligns to 34:373/414 of 6tepA

query
sites
6tepA

V

V

S

W

S

A

A

A

P

P

G

G

K

R

V

V

Y

N

L

L

F

I

G

G

E

E

H

H

A

T

V

D

V

Y

Y

N

G

A

E

G

P

L

A

C

V

L

P

P

C

I

A

A

I

L

E

P

R

H

R

R

A

T

R

F

V

I

E

A

A

L

T

K

Q

P

R

R

S

E

D

D

S

T

R

K

V

V

R

R

V

V

H

-

A

-

Q

-

D

-

L

-

Y

-

L

V

N
x
S

G

G

F
x
V

S

A

V

P

E

D

Y

K

A

V

G

A

P

E

A

A

D

D

D

L

R

D

P

G

T

L

V

K

A

A

T

R

D

G

Q

-

S

-

V

-

L

V

D

D

L

G

G
x
W

M

S

R

A

Y
|
Y

I

P

D

T

G

G

A

V

I

A

D

W

R

A

A

L

R

R

D

Q

L

A

A

G

D

F

Q

D

P

K

E

V

V

K

G

G

L

F

D

D

V

A

T

A

V

F

E

V

S

S

D

C

L

V

P

P

L
|
L

G
|
G

A

S

G
|
G

L

L

G
x
S

S
|
S

A

A

V
x
M

V

T

V

C

A

S

G

T

I

A

D

L

A

A

T

L

A

D

R

D

E

V

L

Y

G

G

L

L

E

G

L

Y

-

G

D

D

S

S

E

D

-

A

-

G

-

R

-

V

E

T

V

L

A

I

N

N

H

A

A

A

Y

I

K

K

V

S

E

E

H

N

E

E

V

M

Q

A

N

G

G

A

E

S

A

T

S

G

R

G

A

L

D

D

T

Q

F

N

C

A

S

S

-

M

-

R

A

C

T

T

G

E

G

G

A

H

A

A

-

L

-

D

-

C

K

R

V

P

E

E

G

L

D

T

S

P

C

L

E

E

T

N

I

V

-

S

-

Q

-

E

-

F

-

D

-

L

D

D

A

K

P

Y

N

N

L

L

P

E

F

L

V

L

V

V

G

-

Y

V

D

D

G

T

G

Q

T

A

G

P

N

H

T

Q

G

G

E

Q

L

Y

-

A

-

Q

-

R

-

A

-

T

-

C

-

E

-

A

-

K

V

I

A

L

G

G

V

V

R

A

A

N

L

L

R

R

-

V

-

T

-

A

-

D

-

G

-

I

-

S

-

K

-

A

-

D

E

D

E

Q

Y

F

D

Q

F

A

A

L

A

K

S

E

T

T

V

L

E

D

S

A

I

L

G

P

D

D

-

E

-

T

L

M

V

K

R

K

R

R

G

V

E

R

H

H

-

V

-

T

-

E

-

I

-

E

-

R

-

V

-

R

-

S

-

F

-

V

-

R

A

A

L

F

D

A

A

Q

G

G

D

D

V

I

D

K

E

A

L

A

G

G

R

R

L

L

M

F

D

N

V

A

N

S

H

H

G

D

L

S

L

L

S

A

A

A

-

D

L

Y

G

E

V

V

S

T

A

V

R

P

S

E

L

L

D

D

A

I

M

A

V

V

W

D

A

V

A

A

R

R

E

K

A

N

G

G

A

A

R

Y

G

G

A

A

K

R

L

M

T

T

G

G

S

G

G

G

G

F

G

G

C

S

I

I

V

I

A

A

L

L

binding adenosine-5'-diphosphate: S80 (≠ N55), V82 (≠ F57), W103 (≠ G81), Y106 (= Y84), L135 (= L113), G136 (= G114), G138 (= G116), S140 (≠ G118), S141 (= S119), S142 (= S120), M145 (≠ V123)

Query Sequence

>WP_007695331.1 NCBI__GCF_000336675.1:WP_007695331.1
MAVSSAPGKVYLFGEHAVVYGEPAVPCAIERRARVEATQRSDSRVRVHAQDLYLNGFSVE
YAGPADDRPTVATDQSVLDLGMRYIDGAIDRARDLADQPEVGLDVTVESDLPLGAGLGSS
AAVVVAGIDATARELGLELDSEEVANHAYKVEHEVQNGEASRADTFCSATGGAAKVEGDS
CETIDAPNLPFVVGYDGGTGNTGELVAGVRALREEYDFAASTVESIGDLVRRGEHALDAG
DVDELGRLMDVNHGLLSALGVSARSLDAMVWAAREAGARGAKLTGSGGGGCIVALDETED
TPTALGYTPECERAFRAELDTEGVRREDG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory