SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_007696022.1 NCBI__GCF_000336675.1:WP_007696022.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9X1K8 4-hydroxy-tetrahydrodipicolinate reductase; HTPA reductase; EC 1.17.1.8 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
36% identity, 98% coverage: 5:253/254 of query aligns to 4:212/216 of Q9X1K8

query
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Q9X1K8

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SGRM 9:12 (≠ TGTM 10:13) binding NAD(+)
GTT 71:73 (= GTT 91:93) binding NAD(+)
AYNF 95:98 (≠ ATNF 115:118) binding NAD(+)
K131 (= K151) binding NAD(+)

1vm6B Crystal structure of dihydrodipicolinate reductase (tm1520) from thermotoga maritima at 2.27 a resolution
36% identity, 98% coverage: 5:253/254 of query aligns to 9:217/218 of 1vm6B

query
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1vm6B

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active site: H132 (= H147), K136 (= K151)
binding nicotinamide-adenine-dinucleotide: G12 (= G8), S14 (≠ T10), G15 (= G11), R16 (≠ T12), M17 (= M13), D37 (= D52), V38 (≠ F53), F53 (= F68), S54 (≠ T69), S55 (≠ G70), E57 (= E72), A58 (≠ S73), G76 (= G91), T78 (= T93), Y101 (≠ T116), N102 (= N117), F103 (= F118), F192 (= F228)

5temA Structure of 4-hydroxy-tetrahydrodipicolinate reductase from vibrio vulnificus with 2,6 pyridine dicarboxylic and nadh (see paper)
36% identity, 100% coverage: 1:253/254 of query aligns to 1:264/266 of 5temA

query
sites
5temA

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active site: H155 (= H147), K159 (= K151)
binding nicotinamide-adenine-dinucleotide: G8 (= G8), G11 (= G11), R12 (≠ T12), M13 (= M13), E34 (= E46), R35 (= R48), F75 (= F68), T76 (= T69), S80 (= S73), G98 (= G91), T100 (= T93), P123 (≠ T116), N124 (= N117), Y125 (≠ F118), F239 (= F228)
binding pyridine-2,6-dicarboxylic acid: T100 (= T93), P123 (≠ T116), H156 (= H148), K159 (= K151), S164 (= S156), G165 (= G157), T166 (= T158)

5tejB Structure of 4-hydroxy-tetrahydrodipicolinate reductase from vibrio vulnificus with 2,5 furan dicarboxylic and nadh (see paper)
36% identity, 100% coverage: 1:253/254 of query aligns to 1:264/269 of 5tejB

query
sites
5tejB

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active site: H155 (= H147), K159 (= K151)
binding 2,5 Furan Dicarboxylic Acid: T100 (= T93), H156 (= H148), K159 (= K151), S164 (= S156), G165 (= G157), T166 (= T158)
binding nicotinamide-adenine-dinucleotide: G8 (= G8), G11 (= G11), R12 (≠ T12), M13 (= M13), E34 (= E46), R35 (= R48), F75 (= F68), T76 (= T69), S80 (= S73), G98 (= G91), T100 (= T93), P123 (≠ T116), N124 (= N117), Y125 (≠ F118), F239 (= F228)

5tejA Structure of 4-hydroxy-tetrahydrodipicolinate reductase from vibrio vulnificus with 2,5 furan dicarboxylic and nadh (see paper)
36% identity, 100% coverage: 1:253/254 of query aligns to 1:264/269 of 5tejA

query
sites
5tejA

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R

D

G

L

V

V

A

A

D

Q

R

Q

V

V

P

P

V

V

L

V

R

M

A

A

T
x
P

N

N

F

Y

S

S

R

V

G

G

V

V

H

N

A

L

L

V

A

F

E

K

A

L

L

L

A

E

E

K

T

A

V

A

E

K

T

V

L

M

P

G

D

D

Y

Y

-

C

D

D

I

I

E

E

L

I

T

V

E

E

T

A

H
|
H

H

H

N

R

R

H

K
|
K

R

V

D

D

A

A

P

P

S

S

G

G

T

T

A

A

T

I

T

G

L

M

L

G

D

E

R

A

I

I

D

A

D

G

A

A

S

M

G

G

A

N

N

K

H

L

D

S

-

D

-

V

R

A

T

V

Y

Y

G

A

R

R

E

E

G

G

-

I

E

T

Q

G

P

E

R

R

D

T

G

K

D

D

E

E

V

I

G

G

V

F

H

A

V

T

R

I

R

R

A

A

G

G

N

D

V

I

R

V

G

G

E

E

H

H

E

T

V

A

L

M

L

F

A

A

G

D

N

I

D

G

E

E

V

R

L

V

T

E

L

I

T

T

H

H

R

K

A

A

E

T

S

D

R

R

G

M

V

T

F

F

A

A

A

N

G

G

A

A

L

V

D

K

A

A

V

V

W

W

L

L

A

H

G

E

R

K

D

P

A

A

G

G

W

F

Y

Y

E

T

F

M

E

T

E

D

V

V

L

L

active site: H155 (= H147), K159 (= K151)
binding nicotinamide-adenine-dinucleotide: G8 (= G8), G11 (= G11), R12 (≠ T12), M13 (= M13), E34 (= E46), R35 (= R48), F75 (= F68), T76 (= T69), S80 (= S73), G98 (= G91), T100 (= T93), P123 (≠ T116)

4ywjA Crystal structure of 4-hydroxy-tetrahydrodipicolinate reductase (htpa reductase) from pseudomonas aeruginosa
39% identity, 75% coverage: 63:253/254 of query aligns to 71:265/268 of 4ywjA

query
sites
4ywjA

D

D

V

V

V

L

V

I

D

D

F
|
F

T
|
T

G

H

P

P

E

S

S
x
V

A

T

V

L

E

K

Y

N

A

I

S

E

A

Q

C

C

A

R

E

K

A

A

D

R

V

R

G

A

F

M

V

V

T

I

G
|
G

T

T

T
|
T

G

G

F

F

D

S

E

A

A

D

H

E

R

K

D

L

V

L

L

L

R

A

G

E

V

A

A

A

D

K

R

D

V

I

P

P

V

I

L

V

R

F

A

A

T
x
A

N
|
N

F
|
F

S

S

R

V

G

G

V

V

H

N

A

L

L

C

A

L

E

K

A

L

L

L

A

D

E

T

T

A

V

A

E

R

T

V

L

L

P

G

D

D

-

E

Y

V

D

D

I

I

E

E

L

I

T

I

E

E

T

A

H
|
H

H

H

N

R

R

H

K
|
K

R

V

D

D

A

A

P

P

S

S

G

G

T

T

A

A

T

L

T

R

L

M

L

G

D

E

R

V

I

V

D

A

D

Q

A

A

S

L

G

G

A

R

N

D

H

L

D

Q

R

E

T

V

-

A

-

V

Y

Y

G

G

R

R

E

E

G

G

E

Q

Q

T

-

G

P

A

R

R

D

A

G

R

D

E

E

T

V

I

G

G

V

F

H

A

V

T

R

V

R

R

A

A

G

G

N

D

V

V

R

V

G

G

E

D

H

H

E

T

V

V

L

L

L

F

A

A

G

A

N

E

D

G

E

E

V

R

L

V

T

E

L

I

T

T

H

H

R

K

A

A

E

S

S

S

R
|
R

G

M

V

T

F
|
F

A

A

A

R

G

G

A

A

L

V

D

R

A

A

V

L

W

W

L

L

A

E

G

G

R

K

D

E

A

N

G

G

W

L

Y

Y

E

D

F

M

E

Q

E

D

V

V

L

L

active site: H156 (= H147), K160 (= K151)
binding nicotinamide-adenine-dinucleotide: F76 (= F68), T77 (= T69), V81 (≠ S73), G99 (= G91), T101 (= T93), A124 (≠ T116), N125 (= N117), F126 (= F118), R237 (= R225), F240 (= F228)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 11, 12, 13, 35, 36

P04036 4-hydroxy-tetrahydrodipicolinate reductase; HTPA reductase; EC 1.17.1.8 from Escherichia coli (strain K12) (see 3 papers)
35% identity, 98% coverage: 4:253/254 of query aligns to 8:268/273 of P04036

query
sites
P04036

V

V

G

A

V

I

T

A

G
|
G

A

A

T

G

G
|
G

T
x
R

M
|
M

G

G

R

R

A

Q

V

L

L

I

D

Q

T

A

A

A

A

L

G

A

R

L

D

E

D

G

T

V

T

Q

V

L

A

G

F

A

A

A

T

L

N
x
E

R
|
R

D

E

P

G

A

S

-

S

-

L

-

G

-

S

-

D

A

A

E

G

H

E

V

L

A

A

G

G

H

A

E

G

L

K

R

T

P

G

A

V

V

T

D

V

F

Q

P

S

D

S

L

L

L

D

A

A

T

V

H

K

A

D

P

D

-

F

D

D

V

V

V

F

V

I

D

D

F

F

T
|
T

G
x
R

P

P

E

E

S

G

A

T

V

L

E

N

Y

H

A

L

S

A

A

F

C

C

A

R

E

Q

A

H

D

G

V

K

G

G

F

M

V

V

T

I

G
|
G

T
|
T

G

G

F

F

D

D

E

E

A

A

H

G

R

K

D

Q

V

A

L

I

R

R

G

D

V

A

A

A

D

A

R

D

V

I

P

A

V

I

L

V

R

F

A
|
A

T
x
A

N
|
N

F
|
F

S

S

R

V

G

G

V

V

H

N

A

V

L

M

A

L

E

K

A

L

L

L

A

E

E

K

T

A

V

A

E

K

T

V

L

M

P

G

D

D

Y

Y

-

T

D

D

I

I

E

E

L

I

T

I

E

E

T

A

H
|
H

H

H

N

R

R

H

K
|
K

R

V

D

D

A

A

P

P

S

S

G

G

T

T

A

A

T

L

T

A

L

M

L

G

D

E

R

A

I

I

D

A

D

H

A

A

S

L

G

D

A

K

N

D

H

L

D

K

-

D

-

C

R

A

T

V

Y

Y

G

S

R

R

E

E

G

G

E

H

Q

T

-

G

P

E

R

R

D

V

G

P

D

G

E

T

V

I

G

G

V

F

H

A

V

T

R

V

R

R

A

A

G

G

N

D

V

I

R

V

G

G

E

E

H

H

E

T

V

A

L

M

L

F

A

A

G

D

N

I

D

G

E

E

V

R

L

L

T

E

L

I

T

T

H

H

R

K

A

A

E

S

S

S

R
|
R

G

M

V

T

F
|
F

A

A

A

N

G

G

A

A

L

V

D

R

A

S

A

A

V

L

W

W

L

L

A

S

G

G

R

K

D

E

A

S

G

G

W

L

Y

F

E

D

F

M

E

R

E

D

V

V

L

L

G12 (= G8) binding NADP(+)
GRM 15:17 (≠ GTM 11:13) binding NAD(+)
RM 16:17 (≠ TM 12:13) binding NADP(+)
E38 (≠ N34) binding NAD(+)
R39 (= R35) binding NADP(+)
TR 80:81 (≠ TG 69:70) binding NAD(+)
GTT 102:104 (= GTT 91:93) binding NAD(+); binding NADP(+)
AANF 126:129 (≠ ATNF 115:118) binding NAD(+)
F129 (= F118) binding NADP(+)
H159 (= H147) mutation H->A,Q: 135 to 200-fold reduction in catalytic activity.
K163 (= K151) binding NAD(+); mutation K->A,C,Q: 625 to 830-fold reduction in catalytic activity.
R240 (= R225) binding NADP(+)
F243 (= F228) binding NAD(+)

1drwA Escherichia coli dhpr/nhdh complex (see paper)
35% identity, 98% coverage: 4:253/254 of query aligns to 7:267/272 of 1drwA

query
sites
1drwA

V

V

G

A

V

I

T

A

G
|
G

A

A

T

G

G
|
G

T
x
R

M
|
M

G

G

R

R

A

Q

V

L

L

I

D

Q

T

A

A

A

A

L

G

A

R

L

D

E

D

G

T

V

T

Q

V

L

A

G

F

A

A

A

T

L

N
x
E

R
|
R

D

E

P

G

A

S

-

S

-

L

-

G

-

S

-

D

A

A

E

G

H

E

V

L

A

A

G

G

H

A

E

G

L

K

R

T

P

G

A

V

V

T

D

V

F

Q

P

S

D

S

L

L

L

D

A

A

T

V

H

K

A

D

P

D

-

F

D

D

V

V

V

F

V

I

D

D

F
|
F

T
|
T

G
x
R

P

P

E

E

S

G

A

T

V

L

E

N

Y

H

A

L

S

A

A

F

C

C

A

R

E

Q

A

H

D

G

V

K

G

G

F

M

V

V

T

I

G
|
G

T
|
T

G

G

F

F

D

D

E

E

A

A

H

G

R

K

D

Q

V

A

L

I

R

R

G

D

V

A

A

A

D

A

R

D

V

I

P

A

V

I

L

V

R

F

A

A

T
x
A

N
|
N

F
|
F

S

S

R

V

G

G

V

V

H

N

A

V

L

M

A

L

E

K

A

L

L

L

A

E

E

K

T

A

V

A

E

K

T

V

L

M

P

G

D

D

Y

Y

-

T

D

D

I

I

E

E

L

I

T

I

E

E

T

A

H
|
H

H

H

N

R

R

H

K
|
K

R

V

D

D

A

A

P

P

S

S

G

G

T

T

A

A

T

L

T

A

L

M

L

G

D

E

R

A

I

I

D

A

D

H

A

A

S

L

G

D

A

K

N

D

H

L

D

K

-

D

-

C

R

A

T

V

Y

Y

G

S

R

R

E

E

G

G

E

H

Q

T

-

G

P

E

R

R

D

V

G

P

D

G

E

T

V

I

G

G

V

F

H

A

V

T

R

V

R

R

A

A

G

G

N

D

V

I

R

V

G

G

E

E

H

H

E

T

V

A

L

M

L

F

A

A

G

D

N

I

D

G

E

E

V

R

L

L

T

E

L

I

T

T

H

H

R

K

A

A

E

S

S

S

R

R

G

M

V

T

F
|
F

A

A

A

N

G

G

A

A

L

V

D

R

A

S

A

A

V

L

W

W

L

L

A

S

G

G

R

K

D

E

A

S

G

G

W

L

Y

F

E

D

F

M

E

R

E

D

V

V

L

L

active site: H158 (= H147), K162 (= K151)
binding nicotinamide purin-6-ol-dinucleotide: G11 (= G8), G14 (= G11), R15 (≠ T12), M16 (= M13), E37 (≠ N34), R38 (= R35), F78 (= F68), T79 (= T69), R80 (≠ G70), G101 (= G91), T102 (= T92), T103 (= T93), A126 (≠ T116), N127 (= N117), F128 (= F118), F242 (= F228)

1dihA Three-dimensional structure of e. Coli dihydrodipicolinate reductase (see paper)
35% identity, 98% coverage: 4:253/254 of query aligns to 7:267/272 of 1dihA

query
sites
1dihA

V

V

G

A

V

I

T

A

G
|
G

A

A

T

G

G
|
G

T
x
R

M
|
M

G

G

R

R

A

Q

V

L

L

I

D

Q

T

A

A

A

A

L

G

A

R

L

D

E

D

G

T

V

T

Q

V

L

A

G

F

A

A

A

T

L

N

E

R
|
R

D

E

P

G

A

S

-

S

-

L

-

G

-

S

-

D

A

A

E

G

H

E

V

L

A

A

G

G

H

A

E

G

L

K

R

T

P

G

A

V

V

T

D

V

F

Q

P

S

D

S

L

L

L

D

A

A

T

V

H

K

A

D

P

D

-

F

D

D

V

V

V

F

V

I

D

D

F
|
F

T
|
T

G
x
R

P

P

E

E

S
x
G

A

T

V

L

E

N

Y

H

A

L

S

A

A

F

C

C

A

R

E

Q

A

H

D

G

V

K

G

G

F

M

V

V

T

I

G
|
G

T

T

T
|
T

G

G

F

F

D

D

E

E

A

A

H

G

R

K

D

Q

V

A

L

I

R

R

G

D

V

A

A

A

D

A

R

D

V

I

P

A

V

I

L

V

R

F

A

A

T

A

N
|
N

F
|
F

S

S

R

V

G

G

V

V

H

N

A

V

L

M

A

L

E

K

A

L

L

L

A

E

E

K

T

A

V

A

E

K

T

V

L

M

P

G

D

D

Y

Y

-

T

D

D

I

I

E

E

L

I

T

I

E

E

T

A

H
|
H

H

H

N

R

R

H

K
|
K

R

V

D

D

A

A

P

P

S

S

G

G

T

T

A

A

T

L

T

A

L

M

L

G

D

E

R

A

I

I

D

A

D

H

A

A

S

L

G

D

A

K

N

D

H

L

D

K

-

D

-

C

R

A

T

V

Y

Y

G

S

R

R

E

E

G

G

E

H

Q

T

-

G

P

E

R

R

D

V

G

P

D

G

E

T

V

I

G

G

V

F

H

A

V

T

R

V

R

R

A

A

G

G

N

D

V

I

R

V

G

G

E

E

H

H

E

T

V

A

L

M

L

F

A

A

G

D

N

I

D

G

E

E

V

R

L

L

T

E

L

I

T

T

H

H

R

K

A

A

E

S

S

S

R
|
R

G

M

V

T

F
|
F

A

A

A

N

G

G

A

A

L

V

D

R

A

S

A

A

V

L

W

W

L

L

A

S

G

G

R

K

D

E

A

S

G

G

W

L

Y

F

E

D

F

M

E

R

E

D

V

V

L

L

active site: H158 (= H147), K162 (= K151)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (= G8), G14 (= G11), R15 (≠ T12), M16 (= M13), R38 (= R35), F78 (= F68), T79 (= T69), R80 (≠ G70), G83 (≠ S73), G101 (= G91), T103 (= T93), N127 (= N117), F128 (= F118), R239 (= R225), F242 (= F228)

1drvA Escherichia coli dhpr/acnadh complex (see paper)
35% identity, 98% coverage: 4:253/254 of query aligns to 5:265/270 of 1drvA

query
sites
1drvA

V

V

G

A

V

I

T

A

G
|
G

A

A

T

G

G
|
G

T
x
R

M
|
M

G

G

R

R

A

Q

V

L

L

I

D

Q

T

A

A

A

A

L

G

A

R

L

D

E

D

G

T

V

T

Q

V

L

A

G

F

A

A

A

T

L

N
x
E

R

R

D

E

P

G

A

S

-

S

-

L

-

G

-

S

-

D

A

A

E

G

H

E

V

L

A

A

G

G

H

A

E

G

L

K

R

T

P

G

A

V

V

T

D

V

F

Q

P

S

D

S

L

L

L

D

A

A

T

V

H

K

A

D

P

D

-

F

D

D

V

V

V

F

V

I

D

D

F
|
F

T
|
T

G
x
R

P

P

E

E

S
x
G

A

T

V

L

E

N

Y

H

A

L

S

A

A

F

C

C

A

R

E

Q

A

H

D

G

V

K

G

G

F

M

V

V

T

I

G
|
G

T

T

G

G

F

F

D

D

E

E

A

A

H

G

R

K

D

Q

V

A

L

I

R

R

G

D

V

A

A

A

D

A

R

D

V

I

P

A

V

I

L

V

R

F

A

A

T
x
A

N

N

F
|
F

S

S

R

V

G

G

V

V

H

N

A

V

L

M

A

L

E

K

A

L

L

L

A

E

E

K

T

A

V

A

E

K

T

V

L

M

P

G

D

D

Y

Y

-

T

D

D

I

I

E

E

L

I

T

I

E

E

T

A

H
|
H

H

H

N

R

R

H

K
|
K

R

V

D

D

A

A

P

P

S

S

G

G

T

T

A

A

T

L

T

A

L

M

L

G

D

E

R

A

I

I

D

A

D

H

A

A

S

L

G

D

A

K

N

D

H

L

D

K

-

D

-

C

R

A

T

V

Y

Y

G

S

R

R

E

E

G

G

E

H

Q

T

-

G

P

E

R

R

D

V

G

P

D

G

E

T

V

I

G

G

V

F

H

A

V

T

R

V

R

R

A

A

G

G

N

D

V

I

R

V

G

G

E

E

H

H

E

T

V

A

L

M

L

F

A

A

G

D

N

I

D

G

E

E

V

R

L

L

T

E

L

I

T

T

H

H

R

K

A

A

E

S

S

S

R
|
R

G

M

V

T

F

F

A

A

A

N

G

G

A

A

L

V

D

R

A

S

A

A

V

L

W

W

L

L

A

S

G

G

R

K

D

E

A

S

G

G

W

L

Y

F

E

D

F

M

E

R

E

D

V

V

L

L

active site: H156 (= H147), K160 (= K151)
binding 3-acetylpyridine adenine dinucleotide: G9 (= G8), G12 (= G11), R13 (≠ T12), M14 (= M13), E35 (≠ N34), F76 (= F68), T77 (= T69), R78 (≠ G70), G81 (≠ S73), G99 (= G91), A124 (≠ T116), F126 (= F118), R237 (= R225)

1druA Escherichia coli dhpr/nadh complex (see paper)
35% identity, 98% coverage: 4:253/254 of query aligns to 5:265/270 of 1druA

query
sites
1druA

V

V

G

A

V

I

T

A

G
|
G

A

A

T

G

G
|
G

T
x
R

M
|
M

G

G

R

R

A

Q

V

L

L

I

D

Q

T

A

A

A

A

L

G

A

R

L

D

E

D

G

T

V

T

Q

V

L

A

G

F

A

A

A

T

L

N
x
E

R
|
R

D

E

P

G

A

S

-

S

-

L

-

G

-

S

-

D

A

A

E

G

H

E

V

L

A

A

G

G

H

A

E

G

L

K

R

T

P

G

A

V

V

T

D

V

F

Q

P

S

D

S

L

L

L

D

A

A

T

V

H

K

A

D

P

D

-

F

D

D

V

V

V

F

V

I

D

D

F
|
F

T
|
T

G
x
R

P

P

E

E

S
x
G

A

T

V

L

E

N

Y

H

A

L

S

A

A

F

C

C

A

R

E

Q

A

H

D

G

V

K

G

G

F

M

V

V

T

I

G
|
G

T
|
T

G

G

F

F

D

D

E

E

A

A

H

G

R

K

D

Q

V

A

L

I

R

R

G

D

V

A

A

A

D

A

R

D

V

I

P

A

V

I

L

V

R

F

A

A

T
x
A

N
|
N

F
|
F

S

S

R

V

G

G

V

V

H

N

A

V

L

M

A

L

E

K

A

L

L

L

A

E

E

K

T

A

V

A

E

K

T

V

L

M

P

G

D

D

Y

Y

-

T

D

D

I

I

E

E

L

I

T

I

E

E

T

A

H
|
H

H

H

N

R

R

H

K
|
K

R

V

D

D

A

A

P

P

S

S

G

G

T

T

A

A

T

L

T

A

L

M

L

G

D

E

R

A

I

I

D

A

D

H

A

A

S

L

G

D

A

K

N

D

H

L

D

K

-

D

-

C

R

A

T

V

Y

Y

G

S

R

R

E

E

G

G

E

H

Q

T

-

G

P

E

R

R

D

V

G

P

D

G

E

T

V

I

G

G

V

F

H

A

V

T

R

V

R

R

A

A

G

G

N

D

V

I

R

V

G

G

E

E

H

H

E

T

V

A

L

M

L

F

A

A

G

D

N

I

D

G

E

E

V

R

L

L

T

E

L

I

T

T

H

H

R

K

A

A

E

S

S

S

R

R

G

M

V

T

F
|
F

A

A

A

N

G

G

A

A

L

V

D

R

A

S

A

A

V

L

W

W

L

L

A

S

G

G

R

K

D

E

A

S

G

G

W

L

Y

F

E

D

F

M

E

R

E

D

V

V

L

L

active site: H156 (= H147), K160 (= K151)
binding nicotinamide-adenine-dinucleotide: G9 (= G8), G12 (= G11), R13 (≠ T12), M14 (= M13), E35 (≠ N34), R36 (= R35), F76 (= F68), T77 (= T69), R78 (≠ G70), G81 (≠ S73), G99 (= G91), T100 (= T92), T101 (= T93), A124 (≠ T116), N125 (= N117), F126 (= F118), F240 (= F228)

1arzA Escherichia coli dihydrodipicolinate reductase in complex with nadh and 2,6 pyridine dicarboxylate (see paper)
35% identity, 98% coverage: 4:253/254 of query aligns to 5:265/270 of 1arzA

query
sites
1arzA

V

V

G

A

V

I

T

A

G

G

A

A

T

G

G

G

T

R

M

M

G

G

R

R

A

Q

V

L

L

I

D

Q

T

A

A

A

A

L

G

A

R

L

D

E

D

G

T

V

T

Q

V

L

A

G

F

A

A

A

T

L

N

E

R

R

D

E

P

G

A

S

-

S

-

L

-

G

-

S

-

D

A

A

E

G

H

E

V

L

A

A

G

G

H

A

E

G

L

K

R

T

P

G

A

V

V

T

D

V

F

Q

P

S

D

S

L

L

L

D

A

A

T

V

H

K

A

D

P

D

-

F

D

D

V

V

V

F

V

I

D

D

F

F

T

T

G

R

P

P

E

E

S

G

A

T

V

L

E

N

Y

H

A

L

S

A

A

F

C

C

A

R

E

Q

A

H

D

G

V

K

G

G

F

M

V

V

T

I

G

G

T

T

G

G

F

F

D

D

E

E

A

A

H

G

R

K

D

Q

V

A

L

I

R

R

G

D

V

A

A

A

D

A

R

D

V

I

P

A

V

I

L

V

R

F

A

A

T

A

N

N

F

F

S

S

R

V

G

G

V

V

H

N

A

V

L

M

A

L

E

K

A

L

L

L

A

E

E

K

T

A

V

A

E

K

T

V

L

M

P

G

D

D

Y

Y

-

T

D

D

I

I

E

E

L

I

T

I

E

E

T

A

H
|
H

N

R

R

H

K
|
K

R

V

D

D

A

A

P

P

S

S

G

G

T

T

A

A

T

L

T

A

L

M

L

G

D

E

R

A

I

I

D

A

D

H

A

A

S

L

G

D

A

K

N

D

H

L

D

K

-

D

-

C

R

A

T

V

Y

Y

G

S

R

R

E

E

G

G

E

H

Q

T

-

G

P

E

R

R

D

V

G

P

D

G

E

T

V

I

G

G

V

F

H

A

V

T

R

V

R

R

A

A

G

G

N

D

V

I

R

V

G

G

E

E

H

H

E

T

V

A

L

M

L

F

A

A

G

D

N

I

D

G

E

E

V

R

L

L

T

E

L

I

T

T

H

H

R

K

A

A

E

S

S

S

R

R

G

M

V

T

F

F

A

A

A

N

G

G

A

A

L

V

D

R

A

S

A

A

V

L

W

W

L

L

A

S

G

G

R

K

D

E

A

S

G

G

W

L

Y

F

E

D

F

M

E

R

E

D

V

V

L

L

active site: H156 (= H147), K160 (= K151)
binding phosphate ion: H157 (= H148), K160 (= K151)

1arzB Escherichia coli dihydrodipicolinate reductase in complex with nadh and 2,6 pyridine dicarboxylate (see paper)
35% identity, 98% coverage: 4:253/254 of query aligns to 4:264/269 of 1arzB

query
sites
1arzB

V

V

G

A

V

I

T

A

G
|
G

A

A

T
x
G

G
|
G

T
x
R

M
|
M

G

G

R

R

A

Q

V

L

L

I

D

Q

T

A

A

A

A

L

G

A

R

L

D

E

D

G

T

V

T

Q

V

L

A

G

F

A

A

A

T

L

N
x
E

R

R

D

E

P

G

A

S

-

S

-

L

-

G

-

S

-

D

A

A

E

G

H

E

V

L

A

A

G

G

H

A

E

G

L

K

R

T

P

G

A

V

V

T

D

V

F

Q

P

S

D

S

L

L

L

D

A

A

T

V

H

K

A

D

P

D

-

F

D

D

V

V

V

F

V

I

D

D

F
|
F

T
|
T

G
x
R

P

P

E

E

S
x
G

A

T

V

L

E

N

Y
x
H

A

L

S

A

A

F

C

C

A

R

E

Q

A

H

D

G

V

K

G

G

F

M

V

V

T

I

G
|
G

T

T

T
|
T

G

G

F

F

D

D

E

E

A

A

H

G

R

K

D

Q

V

A

L

I

R

R

G

D

V

A

A

A

D

A

R

D

V

I

P

A

V

I

L

V

R

F

A

A

T
x
A

N
|
N

F
|
F

S

S

R

V

G

G

V

V

H

N

A

V

L

M

A

L

E

K

A

L

L

L

A

E

E

K

T

A

V

A

E

K

T

V

L

M

P

G

D

D

Y

Y

-

T

D

D

I

I

E

E

L

I

T

I

E

E

T

A

H
|
H

N

R

R

H

K
|
K

R

V

D

D

A

A

P

P

S
|
S

G
|
G

T
|
T

A

A

T

L

T

A

L

M

L

G

D

E

R

A

I

I

D

A

D

H

A

A

S

L

G

D

A

K

N

D

H

L

D

K

-

D

-

C

R

A

T

V

Y

Y

G

S

R

R

E

E

G

G

E

H

Q

T

-

G

P

E

R

R

D

V

G

P

D

G

E

T

V

I

G

G

V

F

H

A

V

T

R

V

R

R

A

A

G

G

N

D

V

I

R

V

G

G

E

E

H

H

E

T

V

A

L

M

L

F

A

A

G

D

N

I

D

G

E

E

V

R

L

L

T

E

L

I

T

T

H

H

R

K

A

A

E

S

S

S

R

R

G

M

V

T

F
|
F

A

A

A

N

G

G

A

A

L

V

D

R

A

S

A

A

V

L

W

W

L

L

A

S

G

G

R

K

D

E

A

S

G

G

W

L

Y

F

E

D

F

M

E

R

E

D

V

V

L

L

active site: H155 (= H147), K159 (= K151)
binding 1,4-dihydronicotinamide adenine dinucleotide: G8 (= G8), G10 (≠ T10), G11 (= G11), R12 (≠ T12), M13 (= M13), E34 (≠ N34), F75 (= F68), T76 (= T69), R77 (≠ G70), G80 (≠ S73), H84 (≠ Y77), G98 (= G91), T100 (= T93), A123 (≠ T116), N124 (= N117), F125 (= F118), F239 (= F228)
binding pyridine-2,6-dicarboxylic acid: T100 (= T93), H156 (= H148), K159 (= K151), S164 (= S156), G165 (= G157), T166 (= T158), F239 (= F228)

3ijpA Crystal structure of dihydrodipicolinate reductase from bartonella henselae at 2.0a resolution (see paper)
31% identity, 98% coverage: 6:253/254 of query aligns to 6:264/266 of 3ijpA

query
sites
3ijpA

V

V

T

V

G
|
G

A

A

T
x
N

G
|
G

T
x
R

M
|
M

G

G

R

R

A

E

V

L

L

I

D

T

T

A

A
x
I

A
x
Q

G

R

R
|
R

D

K

D

D

T
x
V

T

E

V

L

A

C

F

A

A

V

T

L

N

V

R
|
R

D

K

P

G

A

S

E

F

H

V

V

D

A

K

G

D

H

A

E

S

L

I

R

L

P

I

A

G

V

S

D

D

F

F

P

L

D

G

L

V

L

R

A

I

T

T

H

D

A

D

P

P

D

E

V

S

-

A

-

F

-

S

-

N

-

T

-

E

-

G

V

I

V

L

D

D

F
|
F

T
x
S

G
x
Q

P

P

E

Q

S
x
A

A

S

V

V

E

L

Y

Y

A

A

S

N

A

Y

C

A

A

A

E

Q

A

K

D

S

V

L

G

I

F

H

V

I

T

I

G
|
G

T

T

T
|
T

G

G

F

F

D

S

E

K

A

T

H

E

R

E

D

A

V

Q

L

I

R

A

G

D

V

F

A

A

D

K

R

Y

V

T

P

T

V

I

L

V

R

K

A

S

T
x
G

N
|
N

F
x
M

S

S

R

L

G

G

V

V

H

N

A

L

L

L

A

A

E

N

A

L

L

V

A

K

E

R

T

A

V

A

E

K

T

A

L

L

P

D

D

D

-

D

Y

F

D

D

I

I

E

E

L

I

T

Y

E

E

T

M

H
|
H

H

H

N

A

R

N

K
|
K

R

V

D

D

A

S

P

P

S

S

G

G

T

T

A

A

T

-

L

L

D

L

R

G

I

Q

D

A

A

A

S

E

G

G

A

R

N

N

-

I

-

M

-

L

-

K

H

N

D

V

R

S

T

V

Y

N

G

G

R

R

E

S

G

G

E

H

Q

T

-

G

P

K

R

R

D

E

G

K

D

G

E

T

V

I

G

G

V

F

H

A

V

C

R

S

R

R

A

G

G

G

N

T

V

V

R

I

G

G

E

D

H

H

E

S

V

I

L

T

L

F

A

A

G

G

N

E

D

N

E

E

V

R

L

I

T

V

L

L

T

S

H

H

R

I

A

A

E

Q

S

E

R

R

G

S

V

I

F
|
F

A

A

A

N

G

G

A

A

L

L

D

K

A

A

V

L

W

W

L

A

A

K

G

N

R

H

D

E

A

N

G

G

W

L

Y

Y

E

S

F

M

E

L

E

D

V

V

L

L

active site: H155 (= H147), K159 (= K151)
binding sodium ion: I21 (≠ A21), Q22 (≠ A22), R24 (= R24), V27 (≠ T27)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G8), N10 (≠ T10), G11 (= G11), R12 (≠ T12), M13 (= M13), R35 (= R35), F75 (= F68), S76 (≠ T69), Q77 (≠ G70), A80 (≠ S73), G98 (= G91), T100 (= T93), G123 (≠ T116), N124 (= N117), M125 (≠ F118), F239 (= F228)

3ijpB Crystal structure of dihydrodipicolinate reductase from bartonella henselae at 2.0a resolution (see paper)
31% identity, 98% coverage: 6:253/254 of query aligns to 6:264/267 of 3ijpB

query
sites
3ijpB

V

V

T

V

G

G

A

A

T

N

G

G

T

R

M

M

G

G

R

R

A

E

V

L

L

I

D

T

T

A

A
x
I

A
x
Q

G

R

R
|
R

D

K

D

D

T
x
V

T
x
E

V

L

A

C

F

A

A

V

T

L

N

V

R

R

D

K

P

G

A

S

E

F

H

V

V

D

A

K

G

D

H

A

E

S

L

I

R

L

P

I

A

G

V

S

D

D

F

F

P

L

D

G

L

V

L

R

A

I

T

T

H

D

A

D

P

P

D

E

V

S

-

A

-

F

-

S

-

N

-

T

-

E

-

G

V

I

V

L

D

D

F

F

T

S

G

Q

P

P

E

Q

S

A

A

S

V

V

E

L

Y

Y

A

A

S

N

A

Y

C

A

A

A

E

Q

A

K

D

S

V

L

G

I

F

H

V

I

T

I

G

G

T

T

G

G

F

F

D

S

E

K

A

T

H

E

R

E

D

A

V

Q

L

I

R

A

G

D

V

F

A

A

D

K

R

Y

V

T

P

T

V

I

L

V

R

K

A

S

T

G

N

N

F

M

S

S

R

L

G

G

V

V

H

N

A

L

L

L

A

A

E

N

A

L

L

V

A

K

E

R

T

A

V

A

E

K

T

A

L

L

P

D

D

D

-

D

Y

F

D

D

I

I

E

E

L

I

T

Y

E

E

T

M

H
|
H

H

H

N

A

R

N

K
|
K

R

V

D

D

A

S

P

P

S

S

G

G

T

T

A

A

T

-

L

L

D

L

R

G

I

Q

D

A

A

A

S

E

G

G

A

R

N

N

-

I

-

M

-

L

-

K

H

N

D

V

R

S

T

V

Y

N

G

G

R

R

E

S

G

G

E

H

Q

T

-

G

P

K

R

R

D

E

G

K

D

G

E

T

V

I

G

G

V

F

H

A

V

C

R

S

R

R

A

G

G

G

N

T

V

V

R

I

G

G

E

D

H

H

E

S

V

I

L

T

L

F

A

A

G

G

N

E

D

N

E

E

V

R

L

I

T

V

L

L

T

S

H

H

R

I

A

A

E

Q

S

E

R

R

G

S

V

I

F

F

A

A

A

N

G

G

A

A

L

L

D

K

A

A

V

L

W

W

L

A

A

K

G

N

R

H

D

E

A

N

G

G

W

L

Y

Y

E

S

F

M

E

L

E

D

V

V

L

L

active site: H155 (= H147), K159 (= K151)
binding sodium ion: I21 (≠ A21), Q22 (≠ A22), R24 (= R24), V27 (≠ T27), E28 (≠ T28)

5z2fA NADPH/pda bound dihydrodipicolinate reductase from paenisporosarcina sp. Tg-14 (see paper)
24% identity, 100% coverage: 1:253/254 of query aligns to 2:265/265 of 5z2fA

query
sites
5z2fA

M

M

T

I

A

R

V

V

G

A

V

I

T

G

G

G

A

P

T
x
R

G
|
G

T
x
K

M
|
M

G

G

R

Q

A

E

V

A

L

V

D

H

T

T

A

V

A

M

G

N

R

N

D

E

D

N

T

M

-

E

T

L

V

V

A

A

F

V

A

L

T
x
D

N
x
H

R
x
K

D

D

-

I

-

G

-

D

-

L

-

S

P

E

A

S

A

P

E

N

H

F

V

P

A

A

G

S

H

Y

E

E

L

V

R

P

P

V

A

F

V

L

D

N

F

L

P

E

D

S

L

L

L

I

A

V

T

T

H

I

A

K

P

P

D

D

V

V

V

F

V

L

D

D

F
x
L

T
|
T

G

T

P

P

E

H

S

Q

A

V

V

F

E

E

Y

H

A

T

S

M

A

L

C

C

A

L

E

Q

A

N

D

N

V

V

G

R

F

P

V

V

T

I

G
|
G

T
|
T

G

G

F

F

-

T

-

D

D

E

E

Q

A

L

H

Q

R

Q

D

C

V

T

L

I

R

L

G

A

V

E

A

V

D

N

R

K

V

L

P

G

V

C

L

I

R

V

A

A

T
x
P

N
|
N

F
|
F

S

A

R

I

G

G

V

A

H

V

A

L

L

M

A

M

E

K

A

F

L

A

A

S

E

L

T

A

V

A

E

A

T

Y

L

F

P

P

D

-

Y

-

D

D

I

V

E

E

L

I

T

I

E

E

T

M

H
|
H

N

D

R

Q

K
|
K

R

L

D

D

A

A

P

P

S
|
S

G
|
G

T
|
T

A

A

T

Y

T

K

L

T

L

A

D

Q

R

M

I

I

D

A

D

E

A

V

S

R

G

-

A

P

N

S

H

H

D

K

R

Q

T

G

Y

H

G

P

R

N

E

E

G

K

E

E

Q

T

P

L

R

E

D

G

G

A

-

R

-

G

-

A

-

S

-

Y

D

D

E

G

V

I

G

P

V

I

H

H

V

S

R

V

R

R

A

L

G

P

N

G

V

L

R

I

G
x
A

E

H

H

Q

E

Q

V

I

L

L

L

F

A

G

G

G

N

E

D

G

E

Q

V

L

L

F

T

T

L

L

T

R

H

H

R

D

A

S

E

Y

S

N

R

R

G

Q

V

S

F

F

A

M

A

S

G

G

A

V

L

T

D

F

A

S

A

I

V

N

W

Q

L

V

A

M

G

E

R

I

D

K

A

E

G

L

W

V

Y

Y

E

G

F

L

E

E

E

N

V

I

L

L

binding nadp nicotinamide-adenine-dinucleotide phosphate: R11 (≠ T10), G12 (= G11), K13 (≠ T12), M14 (= M13), D35 (≠ T33), H36 (≠ N34), K37 (≠ R35), L76 (≠ F68), T77 (= T69), G99 (= G91), T100 (= T92), T101 (= T93), P126 (≠ T116), N127 (= N117), F128 (= F118)
binding pyridine-2,6-dicarboxylic acid: P126 (≠ T116), H155 (= H147), H156 (= H148), K159 (= K151), S164 (= S156), G165 (= G157), T166 (= T158), A215 (≠ G203)

5z2eA Dipicolinate bound dihydrodipicolinate reductase from paenisporosarcina sp. Tg-14 (see paper)
24% identity, 100% coverage: 1:253/254 of query aligns to 2:265/265 of 5z2eA

query
sites
5z2eA

M

M

T

I

A

R

V

V

G

A

V

I

T

G

G

G

A

P

T

R

G

G

T

K

M

M

G

G

R

Q

A

E

V

A

L

V

D

H

T

T

A

V

A

M

G

N

R

N

D

E

D

N

T

M

-

E

T

L

V

V

A

A

F

V

A

L

T

D

N

H

R

K

D

D

-

I

-

G

-

D

-

L

-

S

P

E

A

S

A

P

E

N

H

F

V

P

A

A

G

S

H

Y

E

E

L

V

R

P

P

V

A

F

V

L

D

N

F

L

P

E

D

S

L

L

L

I

A

V

T

T

H

I

A

K

P

P

D

D

V

V

V

F

V

L

D

D

F

L

T

T

G

T

P

P

E

H

S

Q

A

V

V

F

E

E

Y

H

A

T

S

M

A

L

C

C

A

L

E

Q

A

N

D

N

V

V

G

R

F

P

V

V

T

I

G

G

T

T

G

G

F

F

-

T

-

D

D

E

E

Q

A

L

H

Q

R

Q

D

C

V

T

L

I

R

L

G

A

V

E

A

V

D

N

R

K

V

L

P

G

V

C

L

I

R

V

A

A

T

P

N

N

F

F

S

A

R

I

G

G

V

A

H

V

A

L

L

M

A

M

E

K

A

F

L

A

A

S

E

L

T

A

V

A

E

A

T

Y

L

F

P

P

D

-

Y

-

D

D

I

V

E

E

L

I

T

I

E

E

T

M

H
|
H

N

D

R

Q

K
|
K

R

L

D

D

A

A

P

P

S
|
S

G
|
G

T
|
T

A

A

T

Y

T

K

L

T

L

A

D

Q

R

M

I

I

D

A

D

E

A

V

S

R

G

-

A

P

N

S

H

H

D

K

R

Q

T

G

Y

H

G

P

R

N

E

E

G

K

E

E

Q

T

P

L

R

E

D

G

G

A

-

R

-

G

-

A

-

S

-

Y

D

D

E

G

V

I

G

P

V

I

H

H

V

S

R

V

R

R

A

L

G

P

N

G

V

L

R

I

G

A

E

H

H

Q

E

Q

V

I

L

L

L

F

A

G

G

G

N

E

D

G

E

Q

V

L

L

F

T

T

L

L

T

R

H

H

R

D

A

S

E

Y

S

N

R

R

G

Q

V

S

F

F

A

M

A

S

G

G

A

V

L

T

D

F

A

S

A

I

V

N

W

Q

L

V

A

M

G

E

R

I

D

K

A

E

G

L

W

V

Y

Y

E

G

F

L

E

E

E

N

V

I

L

L

binding pyridine-2,6-dicarboxylic acid: H155 (= H147), H156 (= H148), K159 (= K151), S164 (= S156), G165 (= G157), T166 (= T158)

5wolA Crystal structure of dihydrodipicolinate reductase dapb from coxiella burnetii
27% identity, 98% coverage: 4:253/254 of query aligns to 4:230/230 of 5wolA

query
sites
5wolA

V

V

G

I

V

I

T
x
N

G

G

A

I

T

N

G
|
G

T
x
K

M
|
M

G

G

R

R

A

V

V

V

L

K

D

E

T

N

A

I

A

T

G

A

R

Q

D

S

D

D

T

L

T

E

V

L

A

V

F

S

A

G

T

T

N
x
G

R
|
R

D

Q

P

D

A

-

E

-

H

-

V

-

A

-

G

-

H

-

E

-

L

-

R

-

P

-

A

-

V

-

D

D

F

L

P

A

D

K

L

T

L

I

A

Q

T

T

H

T

A

H

P

A

D

D

V

V

V

I

D

D

F
|
F

T
|
T

G
x
T

P

P

E

Q

S
|
S

A

V

V

F

E

H

Y

N

A

A

S

E

A

I

C

I

A

I

E

Q

A

S

D

G

V

A

G

R

F

P

V

V

T

I

G
|
G

T
|
T

G

G

F

L

D

T

E

L

A

E

H

Q

R

I

D

A

V

L

L

L

-

D

-

K

R

Q

G

C

V

R

A

N

D

K

R

K

V

L

P

G

V

A

L

I

R

V

A

A

T
x
P

N
|
N

F
|
F

S

S

R

V

G

G

V

A

H

V

A

L

L

M

A

M

E

K

A

Y

L

A

A

K

E

E

T

A

V

A

E

H

T

Y

L

F

P

P

D

D

Y

-

D

-

I

V

E

E

L

I

T

I

E

E

T

M

H
|
H

H

H

N

S

R

Q

K
|
K

R

I

D

D

A

A

P

P

S

S

G

G

T
|
T

A

A

T

-

L

-

D

-

R

-

I

I

D
x
K

D

T

A

A

S

Q

G

M

A

I

N

G

H

E

D

M

R

R

T

S

Y

S

G

R

R

A

E

R

G

G

E

E

Q

-

P

I

R

K

D

N

G

G

D

-

E

-

V

I

G

P

V

I

H

H

V

S

R

I

R

R

A

L

G

P

N

G

V

L

R

F

G

S

E

H

H

Q

E

S

V

V

L

I

L

F

A

G

G

S

N

N

D

G

E

E

V

T

L

L

T

T

L

I

T

R

H

H

R

D

A

G

E

M

S

D

R

R

G

N

V

C

F

T

A

M

A

P

G

G

A

I

L

F

D

M

A

A

A

C

V

R

W

K

L

V

A

M

G

E

R

L

D

D

A

Y

G

L

W

V

Y

Y

E

G

F

L

E

E

E

N

V

L

L

L

active site: H133 (= H147), K137 (= K151)
binding pyridine-2-carboxylic acid: P104 (≠ T116), T144 (= T158), K147 (≠ D167)
binding nadp nicotinamide-adenine-dinucleotide phosphate: N7 (≠ T7), G11 (= G11), K12 (≠ T12), M13 (= M13), G34 (≠ N34), R35 (= R35), F54 (= F68), T55 (= T69), T56 (≠ G70), S59 (= S73), G77 (= G91), T78 (= T92), T79 (= T93), P104 (≠ T116), N105 (= N117), F106 (= F118)

5eesA Crystal structure of dapb in complex with NADP+ from corynebacterium glutamicum (see paper)
30% identity, 91% coverage: 4:233/254 of query aligns to 4:225/247 of 5eesA

query
sites
5eesA

V

V

G

G

V

V

T

L

G
|
G

A

A

T

K

G
|
G

T
x
R

M
x
V

G

G

R

Q

A

T

V

I

L

V

D

A

T

A

A

V

A

N

G

E

R

S

D

D

T

L

T

E

V

L

A

V

F

A

A

E

T

I

N
x
G

R
x
V

D

D

P

-

A

-

E

-

H

-

V

-

A

-

G

-

H

-

E

-

L

-

R

-

P

-

A

-

V

-

D

D

F

D

P

L

D

S

L

L

A

V

T

D

H

N

A

G

P

A

D

E

V

V

D

D

F
|
F

T
|
T

G

T

P

P

E

N

S

A

A

V

V

M

E

G

Y

N

A

L

S

E

A

F

C

C

A

I

E

N

A

N

D

G

V

I

G

S

F

A

V

V

T

V

G
|
G

T

T

T
|
T

G

G

F

F

D

D

E

D

A

A

H

R

-

L

-

E

-

Q

-

V

R

R

D

D

V

W

L

L

R

E

G

G

V

-

A

K

D

D

R

N

V

V

P

G

V

V

L

L

R

I

A

A

T
x
P

N
|
N

F
|
F

S

A

R

-

G

-

V

I

H

S

A

A

L

V

A

L

E

T

A

M

L

V

A

F

E

S

T

K

V

Q

E

A

T

A

L

R

P

F

D

F

Y

E

D

S

I

A

E

E

L

V

T

I

E

E

T

L

H
|
H

N

P

R

N

K
|
K

R

L

D

D

A

A

P

P

S

S

G
|
G

T
|
T

A

A

T

I

T

H

L

T

L

A

D

Q

R

G

I

I

D

A

A

A

S

R

G

K

-

E

-

A

-

G

-

M

-

D

A

A

N

Q

H

P

D

D

R

A

T

T

-

E

Y

Q

G

A

R

L

E

E

G

G

E

S

Q

R

P

G

R

A

D

S

G

V

D

D

E

G

V

I

G

P

V

V

H

H

V

A

R

V

R

R

A

M

G

S

N

G

V

M

R

V

G

A

E

H

H

E

E

Q

V

V

L

I

L

F

A

G

G

T

N

Q

D

G

E

Q

V

T

L

L

T

T

L

I

T

K

H

Q

R

D

A

S

E

Y

S

D

R

R

G

N

V

S

F
|
F

A

A

A

P

G

G

A

V

L

L

active site: H133 (= H147), K137 (= K151)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G8), G11 (= G11), R12 (≠ T12), V13 (≠ M13), G34 (≠ N34), V35 (≠ R35), F53 (= F68), T54 (= T69), G76 (= G91), T78 (= T93), P104 (≠ T116), N105 (= N117), F106 (= F118), F220 (= F228)
binding sulfate ion: H134 (= H148), K137 (= K151), K137 (= K151), G143 (= G157), T144 (= T158)

5eerA Crystal structure of dapb from corynebacterium glutamicum (see paper)
30% identity, 91% coverage: 4:233/254 of query aligns to 4:225/247 of 5eerA

query
sites
5eerA

V

V

G

G

V

V

T

L

G

G

A

A

T

K

G

G

T

R

M

V

G

G

R

Q

A

T

V

I

L

V

D

A

T

A

A

V

A

N

G

E

R

S

D

D

T

L

T

E

V

L

A

V

F

A

A

E

T

I

N

G

R

V

D

D

P

-

A

-

E

-

H

-

V

-

A

-

G

-

H

-

E

-

L

-

R

-

P

-

A

-

V

-

D

D

F

D

P

L

D

S

L

L

A

V

T

D

H

N

A

G

P

A

D

E

V

V

D

D

F

F

T

T

G

T

P

P

E

N

S

A

A

V

V

M

E

G

Y

N

A

L

S

E

A

F

C

C

A

I

E

N

A

N

D

G

V

I

G

S

F

A

V

V

T

V

G

G

T

T

G

G

F

F

D

D

E

D

A

A

H

R

-

L

-

E

-

Q

-

V

R

R

D

D

V

W

L

L

R

E

G

G

V

-

A

K

D

D

R

N

V

V

P

G

V

V

L

L

R

I

A

A

T

P

N

N

F

F

S

A

R

-

G

-

V

I

H

S

A

A

L

V

A

L

E

T

A

M

L

V

A

F

E

S

T

K

V

Q

E

A

T

A

L

R

P

F

D

F

Y

E

D

S

I

A

E

E

L

V

T

I

E

E

T

L

H
|
H

N

P

R

N

K
|
K

R

L

D

D

A

A

P

P

S
|
S

G
|
G

T
|
T

A

A

T

I

T

H

L

T

L

A

D

Q

R

G

I

I

D

A

A

A

S

R

G

K

-

E

-

A

-

G

-

M

-

D

A

A

N

Q

H

P

D

D

R

A

T

T

-

E

Y

Q

G

A

R

L

E

E

G

G

E

S

Q

R

P

G

R

A

D

S

G

V

D

D

E

G

V

I

G

P

V

V

H

H

V

A

R

V

R

R

A

M

G

S

N

G

V

M

R

V

G

A

E

H

H

E

E

Q

V

V

L

I

L

F

A

G

G

T

N

Q

D

G

E

Q

V

T

L

L

T

T

L

I

T

K

H

Q

R

D

A

S

E

Y

S

D

R

R

G

N

V

S

F

F

A

A

A

P

G

G

A

V

L

L

active site: H133 (= H147), K137 (= K151)
binding sulfate ion: H134 (= H148), K137 (= K151), K137 (= K151), S142 (= S156), G143 (= G157), T144 (= T158)

Query Sequence

>WP_007696022.1 NCBI__GCF_000336675.1:WP_007696022.1
MTAVGVTGATGTMGRAVLDTAAGRDDTTVAFATNRDPAAEHVAGHELRPAVDFPDLLATH
APDVVVDFTGPESAVEYASACAEADVGFVTGTTGFDEAHRDVLRGVADRVPVLRATNFSR
GVHALAEALAETVETLPDYDIELTETHHNRKRDAPSGTATTLLDRIDDASGANHDRTYGR
EGEQPRDGDEVGVHVRRAGNVRGEHEVLLAGNDEVLTLTHRAESRGVFAAGALDAAVWLA
GRDAGWYEFEEVLA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory