SitesBLAST
Comparing WP_007699692.1 NCBI__GCF_001277175.1:WP_007699692.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P0AEE5 D-galactose/methyl-galactoside binding periplasmic protein MglB; D-galactose-binding periplasmic protein; GBP; D-galactose/D-glucose-binding protein; GGBP from Escherichia coli (strain K12) (see 4 papers)
93% identity, 100% coverage: 1:331/332 of query aligns to 1:332/332 of P0AEE5
- 1:23 (vs. 1:23, 83% identical) signal peptide
- D37 (= D36) binding beta-D-glucose
- N114 (= N113) binding beta-D-glucose
- D157 (= D156) binding Ca(2+)
- N159 (= N158) binding Ca(2+)
- D161 (= D160) binding Ca(2+)
- Q163 (= Q162) binding Ca(2+)
- Q165 (= Q164) binding Ca(2+)
- H175 (= H174) binding beta-D-glucose
- D177 (= D176) binding beta-D-glucose
- R181 (= R180) binding beta-D-glucose
- E228 (= E227) binding Ca(2+)
- N234 (= N233) binding beta-D-glucose
- D259 (= D258) binding beta-D-glucose
- N279 (= N278) binding beta-D-glucose
P23905 D-galactose/methyl-galactoside binding periplasmic protein MglB; D-galactose-binding periplasmic protein; GBP; D-galactose/D-glucose-binding protein; GGBP from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
90% identity, 100% coverage: 1:331/332 of query aligns to 1:332/332 of P23905
- D37 (= D36) binding beta-D-galactose
- N114 (= N113) binding beta-D-galactose
- D157 (= D156) binding Ca(2+)
- N159 (= N158) binding Ca(2+)
- D161 (= D160) binding Ca(2+)
- K163 (≠ Q162) binding Ca(2+)
- Q165 (= Q164) binding Ca(2+)
- H175 (= H174) binding beta-D-galactose
- D177 (= D176) binding beta-D-galactose
- R181 (= R180) binding beta-D-galactose
- E228 (= E227) binding Ca(2+)
- N234 (= N233) binding beta-D-galactose
- D259 (= D258) binding beta-D-galactose
- N279 (= N278) binding beta-D-galactose
2gbpA Sugar and signal-transducer binding sites of the escherichia coli galactose chemoreceptor protein (see paper)
94% identity, 92% coverage: 26:331/332 of query aligns to 4:309/309 of 2gbpA
- binding beta-D-glucopyranose: D14 (= D36), N91 (= N113), K92 (= K114), H152 (= H174), D154 (= D176), R158 (= R180), W183 (= W205), N211 (= N233), D236 (= D258), N256 (= N278)
- binding calcium ion: D134 (= D156), N136 (= N158), D138 (= D160), Q140 (= Q162), Q142 (= Q164), E205 (= E227)
2qw1A Glucose/galactose binding protein bound to 3-o-methyl d-glucose (see paper)
94% identity, 91% coverage: 26:328/332 of query aligns to 3:305/305 of 2qw1A
8fxtA Escherichia coli periplasmic glucose-binding protein glucose complex: acrylodan conjugate attached at w183c (see paper)
94% identity, 92% coverage: 24:328/332 of query aligns to 1:305/305 of 8fxtA
- binding beta-D-glucopyranose: D12 (= D35), D12 (= D35), D13 (= D36), N14 (= N37), D39 (= D62), Q41 (= Q64), N90 (= N113), D120 (= D143), K124 (= K147), H151 (= H174), D153 (= D176), R157 (= R180), D183 (= D206), T184 (= T207), K188 (= K211), D189 (= D212), N210 (= N233), D235 (= D258), N255 (= N278), G296 (= G319), D298 (= D321), N301 (= N324)
- binding calcium ion: D133 (= D156), N135 (= N158), D137 (= D160), Q139 (= Q162), Q141 (= Q164), E204 (= E227)
- binding alpha-D-glucopyranose: D13 (= D36), N90 (= N113), H151 (= H174), D153 (= D176), R157 (= R180), N210 (= N233), D235 (= D258), N255 (= N278)
- binding 1-[6-(dimethylamino)naphthalen-2-yl]propan-1-one: Y9 (= Y32), N42 (= N65), E148 (= E171), M181 (= M204), C182 (≠ W205)
1gcaA The 1.7 angstroms refined x-ray structure of the periplasmic glucose(slash)galactose receptor from salmonella typhimurium (see paper)
92% identity, 92% coverage: 26:331/332 of query aligns to 4:309/309 of 1gcaA
- binding calcium ion: D134 (= D156), N136 (= N158), D138 (= D160), K140 (≠ Q162), Q142 (= Q164), E205 (= E227)
- binding beta-D-galactopyranose: D14 (= D36), F16 (= F38), N91 (= N113), K92 (= K114), H152 (= H174), D154 (= D176), R158 (= R180), N211 (= N233), D236 (= D258), N256 (= N278)
3ga5A X-ray structure of glucose/galactose receptor from salmonella typhimurium in complex with (2r)-glyceryl-beta-d-galactopyranoside (see paper)
92% identity, 92% coverage: 26:329/332 of query aligns to 2:305/305 of 3ga5A
- binding calcium ion: D132 (= D156), N134 (= N158), D136 (= D160), K138 (≠ Q162), Q140 (= Q164), E203 (= E227)
- binding (2R)-2,3-dihydroxypropyl beta-D-galactopyranoside: D12 (= D36), F14 (= F38), N89 (= N113), K90 (= K114), T108 (= T132), H150 (= H174), D152 (= D176), R156 (= R180), W181 (= W205), N209 (= N233), D234 (= D258), N254 (= N278), Q259 (= Q283)
5kwsA Crystal structure of galactose binding protein from yersinia pestis in the complex with beta d glucose
89% identity, 92% coverage: 23:329/332 of query aligns to 1:307/307 of 5kwsA
- binding beta-D-glucopyranose: D14 (= D36), N91 (= N113), K92 (= K114), H152 (= H174), D154 (= D176), R158 (= R180), W183 (= W205), N211 (= N233), D236 (= D258), N256 (= N278)
- binding calcium ion: D134 (= D156), N136 (= N158), D138 (= D160), K140 (≠ Q162), Q142 (= Q164), E205 (= E227)
4z0nA Crystal structure of a periplasmic solute binding protein (ipr025997) from streptobacillus moniliformis dsm-12112 (smon_0317, target efi- 511281) with bound d-galactose
61% identity, 91% coverage: 27:328/332 of query aligns to 4:305/307 of 4z0nA
- active site: T7 (= T30), Q45 (= Q67), S46 (= S68), L89 (≠ F111), V96 (≠ R118), Y107 (= Y129), G109 (= G131), M192 (= M214), S221 (≠ A243)
- binding calcium ion: D134 (= D156), N136 (= N158), D138 (= D160), V140 (≠ Q162), Q142 (= Q164), E205 (= E227)
- binding beta-D-galactopyranose: D13 (= D36), F15 (= F38), N91 (= N113), K92 (= K114), Q152 (≠ H174), D154 (= D176), R158 (= R180), W183 (= W205), N211 (= N233), D235 (= D258), Q255 (≠ N278)
8fxuA Thermoanaerobacter thermosaccharolyticum periplasmic glucose-binding protein glucose complex: badan conjugate attached at f17c (see paper)
50% identity, 91% coverage: 27:327/332 of query aligns to 6:308/310 of 8fxuA
- binding beta-D-glucopyranose: D15 (= D36), N90 (= N113), R91 (≠ K114), D153 (= D176), R157 (= R180), W182 (= W205), N209 (= N233), D237 (= D258)
- binding calcium ion: D133 (= D156), N135 (= N158), D137 (= D160), V139 (≠ Q162), Q141 (= Q164), E203 (= E227)
- binding 2-bromo-1-[6-(dimethylamino)naphthalen-2-yl]ethan-1-one: C17 (≠ F38), D237 (= D258), A238 (= A259), T239 (≠ L260), N257 (= N278)
4yo7A Crystal structure of an abc transporter solute binding protein (ipr025997) from bacillus halodurans c-125 (bh2323, target efi- 511484) with bound myo-inositol
31% identity, 92% coverage: 27:331/332 of query aligns to 9:286/287 of 4yo7A
- binding 1,2,3,4,5,6-hexahydroxy-cyclohexane: D17 (= D35), N94 (= N113), R95 (≠ K114), R147 (= R180), N197 (= N233), D223 (= D258), Q243 (≠ N278)
- binding magnesium ion: L214 (vs. gap), V217 (= V252)
6bgcA The crystal structure of the w145a variant of tpmglb-2 (tp0684) with bound glucose (see paper)
33% identity, 60% coverage: 104:302/332 of query aligns to 1:230/364 of 6bgcA
Sites not aligning to the query:
6bgdA The crystal structure of the w145a variant of tpmglb-2 (tp0684) with bound ligand (see paper)
33% identity, 60% coverage: 103:302/332 of query aligns to 4:234/373 of 6bgdA
A0QYB5 D-threitol-binding protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
30% identity, 82% coverage: 26:298/332 of query aligns to 36:289/349 of A0QYB5
- Y42 (= Y32) binding D-threitol
- N121 (≠ R118) binding D-threitol
- R173 (≠ T181) binding D-threitol
- N224 (= N233) binding D-threitol
- D249 (= D258) binding D-threitol
- Q269 (≠ N278) binding D-threitol
4rsmA Crystal structure of carbohydrate transporter msmeg_3599 from mycobacterium smegmatis str. Mc2 155, target efi-510970, in complex with d-threitol (see paper)
30% identity, 82% coverage: 26:298/332 of query aligns to 4:257/315 of 4rsmA