SitesBLAST

Q48436 Diacetyl reductase [(S)-acetoin forming]; Acetoin(diacetyl) reductase; AR; Meso-2,3-butanediol dehydrogenase; EC 1.1.1.304 from Klebsiella pneumoniae (see paper)
89% identity, 100% coverage: 1:256/256 of query aligns to 1:256/256 of Q48436

query
sites
Q48436

M

M

K

K

V

V

A

A

L
|
L

V
|
V

T
|
T

G
|
G

A
|
A

G
|
G

Q
|
Q

G
|
G

I
|
I

G
|
G

K
|
K

A
|
A

I
|
I

A
|
A

L
|
L

R
|
R

L
|
L

V
|
V

K
|
K

D
|
D

G
|
G

F
|
F

A
|
A

V
|
V

A
|
A

I
|
I

A
|
A

D
|
D

Y

Y

N

N

A

D

E

A

T

T

A

A

N

K

A

A

V

V

A

A

Q

S

E

E

I

I

N

N

E

Q

Q

A

G

G

S

R

A

A

V

M

A

A

V

V

T

K

V

V

D
|
D

V

V

S

S

K

D

R

R

D

D

Q

Q

V

V

F

F

A

A

V

V

E

E

Q

Q

T

A

R

R

K

K

A

T

L

L

G

G

F

F

D

D

V

V

I

I

V

V

N

N

A

A

G

G

I

V

A

A

P

P

S

S

T

T

P

P

I

I

E

E

T

S

I

I

T

T

E

P

E

E

V

I

I

V

D

D

R

K

V

V

Y

Y

N

N

I

I

N

N

V

V

K

K

G

G

V

V

I

I

W

W

G

G

M

I

Q

Q

A

A

I

V

E

E

A

A

F

F

K

K

A

K

E

E

G

G

H

H

G

G

K

K

I

I

N

N

A

A

C

C

S

S

Q

Q

A

A

G

G

H

H

V

V

G

G

N

N

P

P

E

E

L

L

A

A

V

V

Y

Y

S

S

K
|
K

F

F

A

A

V

V

R

R

G

G

L

L

T

T

Q

Q

T

T

A

A

R

R

D

D

L

L

A

A

P

P

L

L

G

G

I

I

T

T

V

V

N

N

G

G

F

Y

C

C

P

P

G

G

I

I

V

V

K

K

T

T

P

P

M

M

W

W

A

A

E

E

I

I

D

D

R

R

Q

Q

I

V

S

S

E

E

A

A

G

G

K

K

P

P

L

L

G

G

Y

Y

G

G

T

T

E

A

E

E

F

F

A

A

K

K

R

R

I

I

T

T

L

L

G

G

R

R

L

L

S

S

E

E

P

P

E

E

D

D

V

V

A

A

C

C

V

V

S

S

F

Y

L

L

A

A

G

S

P

P

D

D

S

S

D

D

Y

Y

M

M

T

T

G

G

Q

Q

S

S

L

L

I

I

D

D

G

G

M

M

V

V

F

F

S

N

6:33 (vs. 6:33, 100% identical) binding NAD(+)
D59 (= D59) binding NAD(+)
K156 (= K156) binding NAD(+)

3wyeA Crystal structure of chimeric engineered (2s,3s)-butanediol dehydrogenase complexed with NAD+
73% identity, 100% coverage: 2:256/256 of query aligns to 1:255/255 of 3wyeA

query
sites
3wyeA

K

K

V

V

A

A

L

L

V

V

T

T

G
|
G

A

A

G

G

Q
|
Q

G
|
G

I
|
I

G

G

K

K

A

A

I

I

A

A

L

L

R

R

L

L

V

V

K

K

D

D

G

G

F

F

A

A

V

V

A

A

I

I

A

A

D
|
D

Y
|
Y

N

N

A

D

E

A

T

T

A

A

N

K

A

A

V

V

A

A

Q

S

E

E

I

I

N

N

E

Q

Q

A

G

G

S

H

A

A

V

V

A

A

V

V

T

K

V
|
V

D
|
D

V
|
V

S

S

K

D

R

R

D

D

Q

Q

V

V

F

F

A

A

V

V

E

E

Q

Q

T

A

R

R

K

K

A

T

L

L

G

G

F

F

D

D

V

V

I

I

V

V

N

N

N
|
N

A
|
A

G

G

I

I

A

A

P

Q

S

I

T

K

P

P

I

L

E

L

T

E

I

V

T

T

E

E

V

D

I

L

D

K

R

Q

V

I

Y

Y

N

S

I

V

N

N

V

V

K

F

G

S

V

V

I

F

W

F

G

G

M

I

Q

Q

A

A

I

V

E

E

A

A

F

F

K

K

A

K

E

E

G

G

H

H

G

G

K

K

I

I

N

N

A

A

C

A

S
|
S

Q

I

A

A

G

A

H

I

V

Q

G

G

N

F

P

P

E

I

L

L

A

S

V

A

Y
|
Y

S

S

S

T

K
|
K

F

F

A

A

V

V

R

R

G

G

L

L

T

T

Q

Q

T

T

A

A

R

R

D

D

L

L

A

A

P

P

L

L

G

G

I

I

T

T

V

V

N

N

G

G

F

Y

C

C

P
|
P

G
|
G

I

I

V
|
V

K

G

T
|
T

P

G

M
|
M

W
|
W

A

E

E

Q

I

I

D

D

R

A

Q

E

I
x
L

S

S

E

K

A

I

A

N

G

G

K

K

P

P

L

I

G

G

Y

E

G

N

T

F

E

K

E

E

F

Y

A

S

K

S

R

S

I

I

T

A

L

L

G

G

R

R

L

P

S

S

E

V

P

P

E

E

D

D

V

V

A

A

A

G

C

L

V

V

S

S

F

F

L

L

A

A

G

S

P

P

D

D

S

S

D

D

Y

Y

M

M

T

T

G

G

Q

Q

S

S

L

L

I

I

D

D

G

G

M

M

V

V

F

F

S

N

active site: G12 (= G13), S138 (= S139), Y151 (= Y152), K155 (= K156), L196 (≠ I197)
binding nicotinamide-adenine-dinucleotide: G8 (= G9), Q11 (= Q12), G12 (= G13), I13 (= I14), D32 (= D33), Y33 (= Y34), V57 (= V58), D58 (= D59), V59 (= V60), N85 (= N86), A86 (= A87), S138 (= S139), Y151 (= Y152), K155 (= K156), P181 (= P182), G182 (= G183), V184 (= V185), T186 (= T187), M188 (= M189), W189 (= W190)

Q9ZNN8 L-2,3-butanediol dehydrogenase; L-BDH; (S,S)-butanediol dehydrogenase; Diacetyl reductase [(S)-acetoin forming]; EC 1.1.1.76; EC 1.1.1.304 from Corynebacterium glutamicum (Brevibacterium saccharolyticum) (see paper)
49% identity, 100% coverage: 1:256/256 of query aligns to 1:258/258 of Q9ZNN8

query
sites
Q9ZNN8

M

M

K

S

K

K

V

V

A

A

L

M

V

V

T

T

G

G

A

G

G

A

Q
|
Q

G
|
G

I
|
I

G

G

K

R

A

G

I

I

A

S

L

E

R

K

L

L

V

A

K

A

D

D

G

G

F

F

A

D

V

I

A

A

I

V

A

A

D
|
D

Y

L

-

P

-

Q

N
x
Q

A

E

E

E

T

Q

A

A

N

A

A

E

V

T

A

I

Q

K

E

L

I

I

N

E

E

A

Q

A

G

D

G

Q

S

K

A

A

V

V

A

F

V

V

T

G

V

L

D
|
D

V
|
V

S

T

K

D

R

K

D

A

Q

N

V

F

F

D

A

S

A

A

V

I

E

D

Q

E

T

A

R

A

K

E

A

K

L

L

G

G

F

F

D

D

V

V

I

L

V

V

N

N

N
|
N

A

A

G

G

I

I

A

A

P

Q

S

I

T

K

P

P

I

L

E

L

T

E

I

V

T

T

E

E

V

D

I

L

D

K

R

Q

V

I

Y

Y

N

S

I

V

N

N

V

V

K

F

G

S

V

V

I

F

W

F

G

G

M

I

Q

Q

A

A

I

S

E

R

A

K

F

F

K

D

A

E

E

L

G

G

H

V

G

K

G

G

K

K

I

I

N

N

A

A

C

A

S

S

Q
x
I

A

A

G

A

H

I

V

Q

G

G

N
x
F

P

P

E

I

L

L

A

S

V

A

Y
|
Y

S

S

S

T

K
|
K

F

F

A

A

V

V

R

R

G

G

L

L

T

T

Q

Q

T

A

A

A

R

Q

D

E

L

L

A

A

P

P

L

K

G

G

I

H

T

T

V

V

N

N

G

A

F

Y

C

A

P
|
P

G
|
G

I
|
I

V
|
V

K
x
G

T
|
T

P

G

M

M

W

W

A

E

E

Q

I

I

D

D

R

A

Q

E

I

L

S

S

E

K

A

I

A

N

G

G

K

K

P

P

L

I

G

G

Y

E

G

N

T

F

E

K

E

E

F

Y

A

S

K

S

R

S

I

I

T

A

L

L

G

G

R

R

L

P

S

S

E

V

P

P

E

E

D

D

V

V

A

A

A

G

C

L

V

V

S

S

F

F

L

L

A

A

G

S

P

E

D

N

S

S

D

N

Y

Y

M

V

T

T

G

G

Q

Q

S

V

L

M

L

L

I

V

D

D

G

G

M

M

V

L

F

Y

S

N

QGI 12:14 (= QGI 12:14) binding NAD(+)
D33 (= D33) binding NAD(+)
Q37 (≠ N35) binding NAD(+)
DV 61:62 (= DV 59:60) binding NAD(+)
N88 (= N86) binding NAD(+)
I142 (≠ Q140) mutation to Q: Loss of L-BD oxidizing activity, and does not gain the ability to use meso-BD as substrate; when associated with N-148.; mutation to Q: Loss of L-BD oxidizing activity. Does not gain the ability to use meso-BD as substrate.
F148 (≠ N146) mutation to N: Loss of L-BD oxidizing activity, and does not gain the ability to use meso-BD as substrate; when associated with Q-142.; mutation to N: Loss of L-BD oxidizing activity. Does not gain the ability to use meso-BD as substrate.
Y154 (= Y152) binding NAD(+)
K158 (= K156) binding NAD(+)
PGIVGT 184:189 (≠ PGIVKT 182:187) binding NAD(+)

3a28C Crystal structure of l-2,3-butanediol dehydrogenase (see paper)
49% identity, 99% coverage: 3:256/256 of query aligns to 2:257/257 of 3a28C

query
sites
3a28C

K

K

V

V

A

A

L

M

V

V

T

T

G
|
G

A

G

G

A

Q
|
Q

G
|
G

I
|
I

G

G

K

R

A

G

I

I

A

S

L

E

R

K

L

L

V

A

K

A

D

D

G

G

F

F

A

D

V

I

A

A

I

V

A

A

D
|
D

Y
x
L

-

P

-

Q

N
x
Q

A

E

E

E

T

Q

A

A

N

A

A

E

V

T

A

I

Q

K

E

L

I

I

N

E

E

A

Q

A

G

D

G

Q

S

K

A

A

V

V

A

F

V

V

T

G

V
x
L

D
|
D

V
|
V

S

T

K

D

R

K

D

A

Q

N

V

F

F

D

A

S

A

A

V

I

E

D

Q

E

T

A

R

A

K

E

A

K

L

L

G

G

F

F

D

D

V

V

I

L

V

V

N

N

N
|
N

A

A

G

G

I

I

A

A

P

Q

S

I

T

K

P

P

I

L

E

L

T

E

I

V

T

T

E

E

V

D

I

L

D

K

R

Q

V

I

Y

Y

N

S

I

V

N

N

V

V

K

F

G

S

V

V

I

F

W

F

G

G

M

I

Q

Q

A

A

I

S

E

R

A

K

F

F

K

D

A

E

E

L

G

G

H

V

G

K

G

G

K

K

I

I

N

N

A

A

C

A

S
|
S

Q

I

A

A

G

A

H

I

V

Q

G

G

N

F

P

P

E

I

L

L

A

S

V

A

Y
|
Y

S

S

S

T

K
|
K

F

F

A

A

V

V

R

R

G

G

L

L

T

T

Q

Q

T

A

A

A

R

Q

D

E

L

L

A

A

P

P

L

K

G

G

I

H

T

T

V

V

N

N

G

A

F

Y

C

A

P
|
P

G

G

I

I

V
|
V

K

G

T
|
T

P

G

M
|
M

W
|
W

A

E

E

Q

I

I

D

D

R

A

Q

E

I
x
L

S

S

E

K

A

I

A

N

G

G

K

K

P

P

L

I

G

G

Y

E

G

N

T

F

E

K

E

E

F

Y

A

S

K

S

R

S

I

I

T

A

L

L

G

G

R

R

L

P

S

S

E

V

P

P

E

E

D

D

V

V

A

A

A

G

C

L

V

V

S

S

F

F

L

L

A

A

G

S

P

E

D

N

S

S

D

N

Y

Y

M

V

T

T

G

G

Q

Q

S

V

L

M

L

L

I

V

D

D

G

G

M

M

V

L

F

Y

S

N

active site: G12 (= G13), S140 (= S139), Y153 (= Y152), K157 (= K156), L198 (≠ I197)
binding nicotinamide-adenine-dinucleotide: G8 (= G9), Q11 (= Q12), I13 (= I14), D32 (= D33), L33 (≠ Y34), Q36 (≠ N35), L59 (≠ V58), D60 (= D59), V61 (= V60), N87 (= N86), S140 (= S139), Y153 (= Y152), K157 (= K156), P183 (= P182), V186 (= V185), T188 (= T187), M190 (= M189), W191 (= W190)

6pejA Structure of sorbitol dehydrogenase from sinorhizobium meliloti 1021 bound to sorbitol
40% identity, 98% coverage: 3:252/256 of query aligns to 7:253/257 of 6pejA

query
sites
6pejA

K

K

V

S

A

A

L

L

V

I

T

T

G

G

A

S

G

A

Q

R

G
|
G

I

I

G

G

K

R

A

A

I

F

A

A

L

E

R

A

L

Y

V

V

K

R

D

E

G

G

F

A

A

T

V

V

A

A

I

I

A

A

D

D

Y

I

N

D

A

I

E

E

T

R

A

A

N

R

A

Q

V

A

A

A

Q

A

E

E

I

I

N

-

E

-

Q

-

G

G

G

P

S

A

A

A

V

Y

A

A

V

V

T

Q

V

M

D

D

V

V

S

T

K

R

R

Q

D

D

Q

S

V

I

F

D

A

A

V

I

E

A

Q

A

T

T

R

V

K

E

A

H

L

A

G

G

F

L

D

D

V

I

I

L

V

V

N

N

A

A

G

A

I

L

A

F

P

D

S

L

T

A

P

P

I

I

E

V

T

E

I

I

T

T

E

R

E

E

V

S

I

Y

D

E

R

K

V

L

Y

F

N

A

I

I

N

N

V

V

K

A

G

G

V

T

I

L

W

F

G

T

M

L

Q

Q

A

A

I

A

E

R

A

Q

F

M

K

I

A

A

E

Q

G

G

H

R

G

G

K

K

I

I

N

N

A

M

C

A

S
|
S

Q
|
Q

A

A

G

G

H

R

V

R

G

G

N
x
E

P

A

E

L

L

V

A

A

V

I

Y
|
Y

S

C

S

A

S

T

K

K

F

A

A

A

V

V

R

I

G

S

L

L

T

T

Q

Q

T

S

A

A

A

G

R

L

D

D

L

L

A

I

P

K

L

H

G

R

I

I

T

N

V

V

N

N

G

A

F

I

C

A

P

P

G

G

I

V

V

V

K

D

T

G

P

E

M
x
H

W
|
W

A

D

E

G

I

V

D

D

R

A

Q

L

I
x
F

S

A

E

R

A

Y

A

E

G

N

K

R

P

P

L

R

G

G

Y

E

G

K

T

K

E

R

E

L

F

V

A

G

K

E

R

A

I

V

T

P

L

F

G

G

R

R

L

M

S

G

E

T

P

A

E

E

D

D

V

L

A

T

A

G

C

M

V

A

S

I

F

F

L

L

A

A

G

S

P

A

D

E

S

S

D

D

Y

Y

M

I

T

V

G

S

Q

Q

S

T

L

Y

L

N

I

V

D

D

G

G

active site: G17 (= G13), S140 (= S139), Y153 (= Y152)
binding sorbitol: Q141 (= Q140), E147 (≠ N146), H190 (≠ M189), W191 (= W190), F198 (≠ I197)

3ak4A Crystal structure of nadh-dependent quinuclidinone reductase from agrobacterium tumefaciens
44% identity, 98% coverage: 3:253/256 of query aligns to 8:255/258 of 3ak4A

query
sites
3ak4A

K

R

V

K

A

A

L

I

V

V

T

T

G

G

A

G

G

S

Q
x
K

G
|
G

I
|
I

G

G

K

A

A

A

I

I

A

A

L

R

R

A

L

L

V

D

K

K

D

A

G

G

F

A

A

T

V

V

A

A

I

I

A

A

D
|
D

Y
x
L

N

D

A

V

E

M

T

A

A

A

N

Q

A

A

V

V

A

V

Q

A

E

G

I

L

N

-

E

E

Q

N

G

G

S

-

A

-

V

F

A

A

V

V

T

E

V
|
V

D
|
D

V
|
V

S

T

K

K

R

R

D

A

Q

S

V

V

F

D

A

A

V

M

E

Q

Q

K

T

A

R

I

K

D

A

A

L

L

G

G

F

F

D

D

V

L

I

L

V

C

N

A

N
|
N

A
|
A

G
|
G

I

V

A

S

P

T

S

M

T

R

P

P

I

A

E

V

T

D

I

I

T

T

E

D

E

E

V

E

I

W

D

D

R

F

V

N

Y

F

N

D

I

V

N

N

V

A

K

R

G

G

V

V

I

F

W

L

G

A

M

N

Q

Q

A

I

A

A

I

C

E

R

A

H

F

F

K

L

A

A

E

S

G

N

H

T

G

K

G

G

K

V

I

I

I

V

N

N

A
x
T

C

A

S
|
S

Q

L

A

A

G

A

H

K

V

V

G

G

N

A

P

P

E

L

L
|
L

A

A

V

H

Y
|
Y

S

S

S

A

S

S

K
|
K

F

F

A

A

V

V

R

F

G

G

L

W

T

T

Q

Q

T

A

A

L

A

A

R

R

D

E

L

M

A

A

P

P

L

K

G

N

I

I

T

R

V

V

N

N

G

C

F

V

C

C

P

P

G
|
G

I

F

V
|
V

K

K

T
|
T

P

A

M
|
M

W

Q

A

-

E

-

I

-

D

E

R

R

Q

E

I

I

-

I

-

W

-
x
E

S

A

E

E

A

L

A

R

G

G

K

M

P

T

L

P

G

E

Y

A

G

V

T

R

E

A

E

E

F

Y

A

V

K

S

R

L

I

T

T

P

L

L

G

G

R

R

L

I

S

E

E

E

P

P

E

E

D

D

V

V

A

A

A

D

C

V

V

V

S

V

F

F

L

L

A

A

G

S

P

D

D

A

S

A

D

R

Y

F

M

M

T

T

G

G

Q

Q

S

G

L

I

L

N

I

V

D

T

G

G

M

V

active site: G18 (= G13), S141 (= S139), L151 (= L149), Y154 (= Y152), K158 (= K156), E199 (vs. gap)
binding nicotinamide-adenine-dinucleotide: K17 (≠ Q12), G18 (= G13), I19 (= I14), D38 (= D33), L39 (≠ Y34), V60 (= V58), D61 (= D59), V62 (= V60), N88 (= N86), A89 (= A87), G90 (= G88), T139 (≠ A137), S141 (= S139), Y154 (= Y152), K158 (= K156), G185 (= G183), V187 (= V185), T189 (= T187), M191 (= M189)

3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
38% identity, 99% coverage: 1:253/256 of query aligns to 3:244/246 of 3osuA

query
sites
3osuA

M

M

K

T

K

K

V

S

A

A

L

L

V

V

T

T

G

G

A

A

G

S

Q

R

G
|
G

I

I

G

G

K

R

A

S

I

I

A

A

L

L

R

Q

L

L

V

A

K

E

D

E

G

G

F

Y

A

N

V

V

A

A

I

V

A

-

D

N

Y

Y

-

A

-

G

N

S

A

K

E

E

T

K

A

A

N

E

A

A

V

V

A

V

Q

E

E

E

I

I

N

K

E

A

Q

K

G

G

G

V

S

D

A

S

V

F

A

A

V

I

T

Q

V

A

D

N

V

V

S

A

K

D

R

A

D

D

Q

E

V

V

F

K

A

A

A

M

V

I

E

K

Q

E

T

V

R

V

K

S

A

Q

L

F

G

G

G

S

F

L

D

D

V

V

I

L

V

V

N

N

N
|
N

A

A

G

G

I

I

A

T

P

R

S

D

T

N

P

L

I

L

E

M

T

R

I

M

T

K

E

E

E

Q

V

E

I

W

D

D

R

D

V

V

Y

I

N

D

I

T

N

N

V

L

K

K

G

G

V

V

I

F

W

N

G

C

M

I

Q

Q

A

K

A

A

I

T

E

P

A

Q

F

M

K

L

A

R

E

Q

G

-

H

R

G

S

G

G

K

A

I

I

N

N

A

L

C

S

S
|
S

Q

V

A

V

G

G

H

A

V

V

G

G

N

N

P

P

E

G

L
x
Q

A

A

V

N

Y
|
Y

S

V

S

A

S

T

K
|
K

F

A

A

G

V

V

R

I

G

G

L

L

T

T

Q

K

T

S

A

A

R

R

D

E

L

L

A

A

P

S

L

R

G

G

I

I

T

T

V

V

N

N

G

A

F

V

C

A

P

P

G

G

I

F

V

I

K

V

T

S

P

D

M

M

W

T

A

D

E

A

I

L

D

S

R

D

Q

E

I

L

S

K

E

-

A

-

G

-

K

-

P

-

L

-

G

-

Y

-

G

-

T

-

E

E

E

Q

F

M

A

L

K

T

R

Q

I

I

T

P

L

L

G

A

R

R

L

F

S

G

E

Q

P

D

E

T

D

D

V

I

A

A

A

N

C

T

V

V

S

A

F

F

L

L

A

A

G

S

P

D

D

K

S

A

D

K

Y

Y

M

I

T

T

G

G

Q

Q

S

T

L

I

L

H

I

V

D

N

G

G

M

M

active site: G15 (= G13), S141 (= S139), Q151 (≠ L149), Y154 (= Y152), K158 (= K156)
binding magnesium ion: N89 (= N86), K158 (= K156)

3sj7A Structure of beta-ketoacetyl-coa reductase (fabg) from staphylococcus aureus complex with NADPH (see paper)
38% identity, 98% coverage: 3:253/256 of query aligns to 2:237/239 of 3sj7A

query
sites
3sj7A

K

K

V

S

A

A

L

L

V

V

T

T

G
|
G

A

A

G
x
S

Q
x
R

G
|
G

I
|
I

G

G

K

R

A

S

I

I

A

A

L

L

R

Q

L

L

V

A

K

E

D

E

G

G

F

Y

A

N

V

V

A

A

I

V

A

-

D
x
N

Y
|
Y

-
x
A

-
x
G

N
x
S

A

K

E

E

T

K

A

A

N

E

A

A

V

V

A

V

Q

E

E

E

I

I

N

K

E

A

Q

K

G

G

G

V

S

D

A

S

V

F

A

A

V

I

T

Q

V
x
A

D
x
N

V
|
V

S

A

K

D

R

A

D

D

Q

E

V

V

F

K

A

A

A

M

V

I

E

K

Q

E

T

V

R

V

K

S

A

Q

L

F

G

G

G

S

F

L

D

D

V

V

I

L

V

V

N

N

N
|
N

A
|
A

G

G

I

I

A

T

P

R

S

D

T

N

P

L

I

L

E

M

T

R

I

M

T

K

E

E

E

Q

V

E

I

W

D

D

R

D

V

V

Y

I

N

D

I
x
T

N

N

V

L

K

K

G

G

V

V

I

F

W

N

G

C

M

I

Q

Q

A

K

A

A

I

T

E

P

A

Q

F

M

K

L

A

R

E

Q

G

-

H

R

G

S

G

G

K

A

I

I

N

N

A

L

C

S

S
|
S

Q

V

A

V

G

G

H

A

V

V

G

G

N

N

P

P

E

G

L
x
Q

A

A

V

N

Y
|
Y

S

V

S

A

S

T

K
|
K

F

A

A

G

V

V

R

I

G

G

L

L

T

T

Q

K

T

S

A

A

R

R

D

E

L

L

A

A

P

S

L

R

G

G

I

I

T

T

V

V

N

N

G

A

F

V

C

A

P
|
P

G
|
G

I

F

V

I

K

V

T

S

P

D

M

M

W

T

A

D

E

E

I

L

D

-

R

-

Q

-

I

-

S

-

E

-

A

-

G

-

K

-

P

-

L

-

G

-

Y

-

G

-

T

K

E

E

E

Q

F

M

A

L

K

T

R

Q

I

I

T

P

L

L

G

A

R

R

L

F

S

G

E

Q

P

D

E

T

D

D

V

I

A

A

A

N

C

T

V

V

S

A

F

F

L

L

A

A

G

S

P

D

D

K

S

A

D

K

Y

Y

M

I

T

T

G

G

Q

Q

S

T

L

I

L

H

I

V

D

N

G

G

M

M

active site: G12 (= G13), S138 (= S139), Q148 (≠ L149), Y151 (= Y152), K155 (= K156)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G9), S10 (≠ G11), R11 (≠ Q12), I13 (= I14), N31 (≠ D33), Y32 (= Y34), A33 (vs. gap), G34 (vs. gap), S35 (≠ N35), A58 (≠ V58), N59 (≠ D59), V60 (= V60), N86 (= N86), A87 (= A87), T109 (≠ I109), S138 (= S139), Y151 (= Y152), K155 (= K156), P181 (= P182), G182 (= G183)

4nbuB Crystal structure of fabg from bacillus sp (see paper)
37% identity, 99% coverage: 3:255/256 of query aligns to 8:244/244 of 4nbuB

query
sites
4nbuB

K

K

V

V

A

A

L

I

V

I

T

T

G
|
G

A

A

G

A

Q
x
N

G
|
G

I
|
I

G

G

K

L

A

E

I

A

A

A

L

R

R

V

L

F

V

M

K

K

D

E

G

G

F

A

A

K

V

V

A

V

I

I

A

A

D
|
D

Y
x
F

N

N

A

-

E

-

T

-

A

-

N

E

A

A

V

A

A

G

Q

K

E

E

I

A

N

V

E

E

Q

A

G

N

G

P

S

G

A

V

V

V

A

F

V

I

T

R

V
|
V

D
|
D

V
|
V

S

S

K

D

R

R

D

E

Q

S

V

V

F

H

A

R

A

L

V

V

E

E

Q

N

T

V

R

A

K

E

A

R

L

F

G

G

G

K

F

I

D

D

V

I

I

L

V

I

N

N

N
|
N

A
|
A

G
|
G

I
|
I

A

T

P

R

S
x
D

T

S

P

M

I

L

E

S

T
x
K

I

M

T

T

E

V

E

D

V

Q

I

F

D

Q

R

Q

V

V

Y

I

N

N

I

V

N
|
N

V

L

K

T

G

G

V

V

I

F

W

H

G

C

M

T

Q

Q

A

A

A

V

I

L

E

-

A

P

F

Y

K

M

A

A

E

E

G

Q

H

G

G

K

G

G

K

K

I

I

N

N

A
x
T

C

S

S
|
S

Q

V

A

T

G

G

H

T

V

Y

G

G

N
|
N

P
x
V

E

G

L
x
Q

A

T

V

N

Y
|
Y

S

A

K
|
K

F

A

A

G

V

V

R

I

G

G

L

M

T

T

Q

K

T

T

A

W

A

A

R

K

D

E

L

L

A

A

P

R

L

K

G

G

I

I

T

N

V

V

N

N

G

A

F

V

C

A

P
|
P

G

G

I
x
F

V
x
T

K

E

T
|
T

P

A

M
|
M

W

V

A

A

E

E

I

V

D

P

R

E

Q

K

I

V

S

-

E

-

A

-

G

-

K

-

P

-

L

-

G

-

Y

-

G

-

T

I

E

E

E
x
K

F

M

A

K

K

A

R

Q

I

V

T

P

L

M

G

G

R

R

L

L

S

G

E

K

P

P

E

E

D

D

V

I

A

A

A

N

C

A

V

Y

S

L

F

F

L

L

A

A

G

S

P

H

D

E

S

S

D

D

Y

Y

M

V

T

N

G

G

Q

H

S

V

L

L

L

H

I

V

D

D

G

G

M

I

V

M

F

M

active site: G18 (= G13), N111 (= N110), S139 (= S139), Q149 (≠ L149), Y152 (= Y152), K156 (= K156)
binding acetoacetyl-coenzyme a: D93 (≠ S92), K98 (≠ T97), S139 (= S139), N146 (= N146), V147 (≠ P147), Q149 (≠ L149), Y152 (= Y152), F184 (≠ I184), M189 (= M189), K200 (≠ E211)
binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G9), N17 (≠ Q12), G18 (= G13), I19 (= I14), D38 (= D33), F39 (≠ Y34), V59 (= V58), D60 (= D59), V61 (= V60), N87 (= N86), A88 (= A87), G89 (= G88), I90 (= I89), T137 (≠ A137), S139 (= S139), Y152 (= Y152), K156 (= K156), P182 (= P182), F184 (≠ I184), T185 (≠ V185), T187 (= T187), M189 (= M189)

3iccA Crystal structure of a putative 3-oxoacyl-(acyl carrier protein) reductase from bacillus anthracis at 1.87 a resolution (see paper)
38% identity, 98% coverage: 3:252/256 of query aligns to 8:252/255 of 3iccA

query
sites
3iccA

K

K

V

V

A

A

L

L

V

V

T

T

G
|
G

A

A

G
x
S

Q
x
R

G
|
G

I
|
I

G

G

K

R

A

A

I

I

A

A

L

K

R

R

L

L

V

A

K

N

D

D

G

G

F

A

A

L

V

V

A

A

I

I

A
x
H

D
x
Y

Y
x
G

N

N

-

R

A

K

E

E

T

E

A

A

N

E

A

E

V

T

A

V

Q

Y

E

E

I

I

N

Q

E

S

Q

N

G

G

S

S

A

A

V

F

A

S

V

I

T

G

V

A

D

N

V
x
L

S
x
E

K

S

R

L

-

H

-

G

-

V

D

E

Q

A

V

L

F

Y

A

S

V

L

E

D

-

N

-

E

-

L

Q

Q

T

N

R

R

K

T

A

G

L

S

G

T

G

K

F

F

D

D

V

I

I

L

V

I

N

N

N
|
N

A

A

G
|
G

I

I

A

G

P

P

S

G

T

A

P

F

I

I

E

E

T

E

I

T

T

T

E

E

E

Q

V

F

I

F

D

D

R

R

V

M

Y

V

N

S

I

V

N

N

V

A

K

K

G

A

V

P

I

F

W

F

G

I

M

I

Q

Q

A

Q

A

A

I

L

E

S

A

R

F

L

K

R

A

-

E

-

G

-

H

D

G

N

G

S

K

R

I

I

N

N

A
x
I

C

S

S
|
S

Q

A

A

A

G

T

H

R

V

I

G

S

N

L

P

P

E

D

L
x
F

A

I

V

A

Y
|
Y

S

S

S

M

S

T

K
|
K

F

G

A

A

V

I

R

N

G

T

L

M

T

T

Q

F

T

T

A

L

A

A

R

K

D

Q

L

L

A

G

P

A

L

R

G

G

I

I

T

T

V

V

N

N

G

A

F

I

C

L

P
|
P

G
|
G

I

F

V

V

K

K

T

T

P

D

M
|
M

W
x
N

A

A

E

E

I

L

D

-

R

-

Q

-

I

-

S

-

E

-

A

-

A

L

G

S

K

D

P

P

L

M

G

-

Y

-

G

-

T

M

E

K

E

Q

F

Y

A

A

K

T

R

T

I

I

T

S

-

A

L

F

G

N

R

R

L

L

S

G

E

E

P

V

E

E

D

D

V

I

A

A

A

D

C

T

V

A

S

A

F

F

L

L

A

A

G

S

P

P

D

D

S

S

D

R

Y

W

M

V

T

T

G

G

Q

Q

S

L

L

I

L

D

I

V

D

S

G

G

active site: G18 (= G13), S148 (= S139), F158 (≠ L149), Y161 (= Y152), K165 (= K156)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G14 (= G9), S16 (≠ G11), R17 (≠ Q12), G18 (= G13), I19 (= I14), H37 (≠ A32), Y38 (≠ D33), G39 (≠ Y34), L66 (≠ V60), E67 (≠ S61), N98 (= N86), G100 (= G88), I146 (≠ A137), S148 (= S139), Y161 (= Y152), K165 (= K156), P191 (= P182), G192 (= G183), M198 (= M189), N199 (≠ W190)

4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
40% identity, 99% coverage: 3:255/256 of query aligns to 6:247/247 of 4jroC

query
sites
4jroC

K

K

V

V

A

A

L

V

V

V

T

T

G
|
G

A

G

G
x
S

Q
x
R

G
|
G

I
|
I

G

G

K

R

A

D

I

I

A

A

L

I

R

N

L

L

V

A

K

K

D

E

G

G

F

-

A

A

V

N

A

I

I

F

A

F

D
x
N

Y
|
Y

N
|
N

A
x
G

-
x
S

-

P

E

E

T

A

A

A

N

E

A

E

V

T

A

A

Q

K

E

L

I

V

N

A

E

E

Q

H

G

G

G

V

S

E

A

V

V

E

A

A

V

M

T

K

V

A

D
x
N

V
|
V

S

A

K

I

R

A

D

E

Q

D

V

V

F

D

A

A

A

F

V

F

E

K

Q

Q

T

A

R

I

K

E

A

R

L

F

G

G

G

R

F

V

D

D

V

I

I

L

V

V

N

N

N
|
N

A
|
A

G

G

I
|
I

A

T

P

R

S

D

T

N

P

L

I

L

E

M

T

R

I

M

T

K

E

E

E

D

V

E

I

W

D

D

R

D

V

V

Y

I

N

N

I
|
I

N

N

V

L

K

K

G

G

V

T

I

F

W

L

G

C

M

T

Q

K

A

A

A

V

I

S

E

R

A

T

F

M

K

M

A

K

E

Q

G

-

H

R

G

A

G

G

K

K

I

I

N

N

A

M

C

A

S
|
S

Q

V

A

V

G

G

H

L

V

I

G

G

N

N

P

A

E

G

L
x
Q

A

A

V

N

Y
|
Y

S

V

S

A

S

S

K
|
K

F

A

A

G

V

V

R

I

G

G

L

L

T

T

Q

K

T

T

A

T

A

A

R

R

D

E

L

L

A

A

P

P

L

R

G

G

I

I

T

N

V

V

N

N

G

A

F

V

C

A

P
|
P

G

G

I

F

V
x
I

K

T

T
|
T

P

D

M

M

W

T

A

D

E

K

I

L

D

D

R

E

Q

K

I

T

S

K

E

E

A

A

-

M

-

L

A

A

G

Q

K

I

P

P

L

L

G

G

-

A

Y

Y

G

G

T

T

E

-

F

-

A

-

K

-

R

-

I

-

T

-

L

-

G

-

R

-

L

-

S

-

E

-

P

T

E

E

D

D

V

I

A

A

A

N

C

A

V

V

S

L

F

F

L

L

A

A

G

S

P

D

D

A

S

S

D

K

Y

Y

M

I

T

T

G

G

Q

Q

S

T

L

L

L

S

I

V

D

D

G

G

M

M

V

V

F

M

active site: G16 (= G13), S142 (= S139), Q152 (≠ L149), Y155 (= Y152), K159 (= K156)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G9), S14 (≠ G11), R15 (≠ Q12), G16 (= G13), I17 (= I14), N35 (≠ D33), Y36 (= Y34), N37 (= N35), G38 (≠ A36), S39 (vs. gap), N63 (≠ D59), V64 (= V60), N90 (= N86), A91 (= A87), I93 (= I89), I113 (= I109), S142 (= S139), Y155 (= Y152), K159 (= K156), P185 (= P182), I188 (≠ V185), T190 (= T187)

Q9KJF1 (2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA dehydrogenase subunit BbsD; (S,R)-2-(alpha-hydroxybenzyl)succinyl-CoA dehydrogenase subunit BbsD; EC 1.1.1.429 from Thauera aromatica (see 2 papers)
35% identity, 98% coverage: 2:252/256 of query aligns to 5:242/248 of Q9KJF1

query
sites
Q9KJF1

K

N

K

R

V

V

A

A

L

L

V

I

T

T

G

G

A

S

G

A

Q
x
S

G

G

I

M

G

G

K

K

A

Q

I

T

A

A

L

L

R

R

L

F

V

A

K

E

D

Q

G

G

F

A

A

A

V

V

A

V

I

I

A

N

D
|
D

Y

I

N

D

A

A

E

E

T

K

A

V

N

R

A

A

V

T

A

V

Q

D

E

E

I

F

N

S

E

A

Q

R

G

G

G

H

S

R

A

V

V

L

A

G

V

A

T

V

V

A

D
|
D

V
x
I

S

G

K

N

R

K

D

A

Q

A

V

V

F

D

A

G

A

M

V

V

E

K

Q

Q

T

T

R

I

K

D

A

A

L

F

G

G

G

R

F

I

D

D

V

I

I

L

V

V

N

N

N
|
N

A

A

G

G

I

M

A

E

P

R

S

A

T

G

P

A

I

L

E

R

T

K

I

L

T

S

E

E

E

A

V

D

I

W

D

D

R

V

V

T

Y

I

N

N

I

V

N

N

V

L

K

K

G

G

V

T

I

F

W

L

G

C

M

T

Q

Q

A

-

A

A

I

V

E

H

A

G

F

H

K

M

A

V

E

E

G

N

H

K

G

H

G

G

K

R

I

I

I

V

N

N

A

I

C

A

S

S

Q

R

A

A

G

-

H

W

V

L

G

G

N

G

P

A

E

G

L

Q

A

T

V

P

Y
|
Y

S

S

S

A

K
|
K

F

A

A

G

V

V

R

V

G

G

L

M

T

T

Q

R

T

A

A

L

A

A

R

I

D

E

L

L

A

G

P

R

L

A

G

G

I

I

T

T

V

V

N

N

G

C

F

V

C

A

P

P

G

G

I

L

V

I

K

H

T

T

P

P

M

M

W

W

A

D

E

E

I

L

-

P

-

E

-

K

D

D

R

Q

Q

Q

I

F

S

-

E

-

A

-

G

-

K

-

P

-

L

-

G

-

Y

-

G

-

T

-

E

-

F

L

A

L

K

S

R

R

I

Q

T

P

L

T

G

G

R

K

L

L

S

G

E

E

P

P

E

D

D

D

V

I

A

A

A

N

C

T

V

L

S

L

F

F

L

L

A

A

G

D

P

D

D

D

S

S

D

G

Y

F

M

V

T

T

G

G

Q

Q

S

V

L

L

L

Y

I

V

D

C

G

G

S15 (≠ Q12) binding NAD(+)
D36 (= D33) binding NAD(+)
D62 (= D59) binding NAD(+)
I63 (≠ V60) binding NAD(+)
N89 (= N86) binding NAD(+)
Y153 (= Y152) binding NAD(+)
K157 (= K156) binding NAD(+)

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

8dt1C Crystal structure of a putative d-beta-hydroxybutyrate dehydrogenase from burkholderia cenocepacia j2315 in complex with NAD
38% identity, 98% coverage: 3:252/256 of query aligns to 5:255/259 of 8dt1C

query
sites
8dt1C

K

K

V

T

A

A

L

V

V

V

T

T

G
|
G

A

A

G

A

Q
x
S

G
|
G

I
|
I

G

G

K

K

A

E

I

I

A

A

L

L

R

E

L

L

V

A

K

K

D

A

G

G

F

A

A

A

V

V

A

A

I

I

A

A

D
|
D

Y
x
L

N

N

A

Q

E

D

T

G

A

A

N

N

A

A

V

V

A

A

Q

D

E

E

I

I

N

N

E

K

Q

A

G

G

S

K

A

A

V

I

A

G

V

V

T

A

V
x
M

D

D

V
|
V

S

T

K

N

R

E

D

E

Q

A

V

V

F

N

A

T

A

G

V

I

E

D

Q

K

T

V

R

A

K

E

A

A

L

F

G

G

G

S

F

V

D

D

V

I

I

L

V

V

N

S

N
|
N

A
|
A

G

G

I

I

A

Q

P

I

S

V

T

N

P

P

I

I

E

E

T

N

I

Y

T

S

E

F

E

A

V

D

I

W

D

K

R

K

V

M

Y

Q

N

A

I

I

N

H

V

V

K

D

G

G

V

A

I

F

W

L

G

T

M

T

Q

K

A

A

I

L

E

K

A

H

F

M

K

Y

A

K

E

D

G

D

H

R

G

G

K

V

I

V

I

I

N

Y

A
x
M

C

G

S

S

Q

V

A

H

G

S

H

H

V

E

G

A

N

S

P

P

E

L

L

K

A

S

V

A

Y
|
Y

S

V

S

T

S

A

K
|
K

F

H

A

G

V

L

R

L

G

G

L

L

T

A

Q

R

T

V

A

L

A

A

R

K

D

E

L

G

A

A

P

K

L

H

G

N

I

V

T

R

V

S

N

H

G

V

F

V

C

C

P
|
P

G
|
G

I
x
F

V
|
V

K

R

T
|
T

P

P

M

L

W

-

A

-

E

-

I

V

D

D

R

K

Q

Q

I

I

S

P

E

E

A

Q

A

A

G

-

K

K

P

E

L

L

G

G

Y

I

G

S

T

E

E

E

F

V

A

I

K

K

R

K

I

V

T

M

L

L

G

G

R

N

-

T

-

V

-

D

-

G

-

V

L

F

S

T

E

T

P

V

E

Q

D

D

V

V

A

A

A

Q

C

T

V

V

S

L

F

F

L

L

A

S

G

A

P

F

D

P

S

S

D

A

Y

A

M

L

T

T

G

G

Q

Q

S

S

L

F

L

I

I

V

D

S

G

H

G

G

binding nicotinamide-adenine-dinucleotide: G11 (= G9), S14 (≠ Q12), G15 (= G13), I16 (= I14), D35 (= D33), L36 (≠ Y34), M60 (≠ V58), V62 (= V60), N88 (= N86), A89 (= A87), M139 (≠ A137), Y154 (= Y152), K158 (= K156), P184 (= P182), G185 (= G183), F186 (≠ I184), V187 (= V185), T189 (= T187)

7pcsB Structure of the heterotetrameric sdr family member bbscd (see paper)
35% identity, 98% coverage: 2:252/256 of query aligns to 4:241/247 of 7pcsB

query
sites
7pcsB

K

N

K

R

V

V

A

A

L

L

V

I

T

T

G
|
G

A

S

G

A

Q

S

G

G

I
x
M

G

G

K

K

A

Q

I

T

A

A

L

L

R

R

L

F

V

A

K

E

D

Q

G

G

F

A

A

A

V

V

A

V

I

I

A

N

D
|
D

Y
x
I

N

D

A

A

E

E

T

K

A

V

N

R

A

A

V

T

A

V

Q

D

E

E

I

F

N

S

E

A

Q

R

G

G

G

H

S

R

A

V

V

L

A

G

V

A

T

V

V

A

D

D

V
x
I

S

G

K

N

R

K

D

A

Q

A

V

V

F

D

A

G

A

M

V

V

E

K

Q

Q

T

T

R

I

K

D

A

A

L

F

G

G

G

R

F

I

D

D

V

I

I

L

V

V

N

N

N
|
N

A

A

G
|
G

I

M

A

E

P

R

S

A

T

G

P

A

I

L

E

R

T

K

I

L

T

S

E

E

E

A

V

D

I

W

D

D

R

V

V

T

Y

I

N

N

I

V

N

N

V

L

K

K

G

G

V

T

I

F

W

L

G

C

M

T

Q

Q

A

-

A

A

I

V

E

H

A

G

F

H

K

M

A

V

E

E

G

N

H

K

G

H

G

G

K

R

I

I

I

V

N

N

A
x
I

C

A

S
|
S

Q

R

A

A

G

-

H

W

V

L

G

G

N

G

P

A

E

G

L

Q

A

T

V

P

Y
|
Y

S

S

S

A

K
|
K

F

A

A

G

V

V

R

V

G

G

L

M

T

T

Q

R

T

A

A

L

A

A

R

I

D

E

L

L

A

G

P

R

L

A

G

G

I

I

T

T

V

V

N

N

G

C

F

V

C

A

P

P

G

G

I

L

V
x
I

K

H

T

T

P

P

M

M

W

W

A

D

E

E

I

L

-

P

-

E

-

K

D

D

R

Q

Q

Q

I

F

S

-

E

-

A

-

G

-

K

-

P

-

L

-

G

-

Y

-

G

-

T

-

E

-

F

L

A

L

K

S

R

R

I

Q

T

P

L

T

G

G

R

K

L

L

S

G

E

E

P

P

E

D

D

D

V

I

A

A

A

N

C

T

V

L

S

L

F

F

L

L

A

A

G

D

P

D

D

D

S

S

D

G

Y

F

M

V

T

T

G

G

Q

Q

S

V

L

L

L

Y

I

V

D

C

G

G

binding nicotinamide-adenine-dinucleotide: G11 (= G9), M16 (≠ I14), D35 (= D33), I36 (≠ Y34), I62 (≠ V60), N88 (= N86), G90 (= G88), I138 (≠ A137), S140 (= S139), Y152 (= Y152), K156 (= K156), I185 (≠ V185)

7v0hG Crystal structure of putative glucose 1-dehydrogenase from burkholderia cenocepacia in complex with NADP and a potential reaction product
40% identity, 98% coverage: 3:253/256 of query aligns to 12:252/253 of 7v0hG

query
sites
7v0hG

K

K

V

V

A

A

L

I

V

V

T

T

G
|
G

A

A

G
x
S

Q
x
K

G
|
G

I
|
I

G

G

K

A

A

A

I

I

A

A

L

K

R

A

L

L

V

A

K

D

D

E

G

G

F

A

A

A

V

V

A

V

I

V

A

N

D

Y

Y
x
A

N
x
S

A
x
S

E

K

T

A

-

G

A

A

N

D

A

A

V

V

A

V

Q

S

E

A

I

I

N

T

E

E

Q

A

G

G

S

R

A

A

V

V

A

A

V

V

T

G

V
x
G

D
|
D

V
|
V

S

S

K

K

R

A

D

A

Q

D

V

A

F

Q

A

R

A

I

V

V

E

D

Q

T

T

A

R

I

K

E

A

T

L

Y

G

G

G

R

F

L

D

D

V

V

I

L

V

V

N

N

N
|
N

A
x
S

G
|
G

I

V

A
x
Y

P

E

S

F

T

A

P

P

I

I

E

E

T

A

I

I

T

T

E

E

V

H

I

Y

D

R

R

R

V

Q

Y

F

N

D

I

T

N

N

V

V

K

F

G

G

V

V

I

L

W

L

G

T

M

T

Q

Q

A

A

I

V

E

K

A

H

F

L

K

-

A

-

E

-

G

G

H

E

G

G

G

A

K

S

I

I

N

N

A
x
I

C

S

S
|
S

Q

V

A

V

G

T

H

S

V

I

G

T

N

P

P

P

E

A

L

S

A

A

V

V

Y
|
Y

S

S

S

G

S

T

K
|
K

F

G

A

A

V

V

R

D

G

A

L

I

T

T

Q

G

T

V

A

L

A

A

R

L

D

E

L

L

A

G

P

P

L

R

G

K

I

I

T

R

V

V

N

N

G

A

F

I

C

N

P
|
P

G
|
G

I
x
M

V
x
I

K

V

T
|
T

P

E

M
x
G

W
x
T

A

H

E

S

I

A

D

G

R
x
I

Q

I

I

G

S

S

E

D

A

L

A

E

G

A

K

Q

P

V

L

L

G

G

Y

-

G

-

T

-

E

-

F

-

A

-

K

-

R

Q

I

T

T

P

L

L

G

G

R

R

L

L

S

G

E

E

P

P

E

N

D

D

V

I

A

A

A

S

C

V

V

A

S

V

F

F

L

L

A

A

G

S

P

D

D

D

S

A

D

R

Y

W

M

M

T

T

G

G

Q

E

S

H

L

L

L

V

I

V

D

S

G

G

M

L

binding nadp nicotinamide-adenine-dinucleotide phosphate: G18 (= G9), S20 (≠ G11), K21 (≠ Q12), G22 (= G13), I23 (= I14), A43 (≠ Y34), S44 (≠ N35), S45 (≠ A36), G68 (≠ V58), D69 (= D59), V70 (= V60), N96 (= N86), S97 (≠ A87), G98 (= G88), Y100 (≠ A90), I144 (≠ A137), S146 (= S139), Y159 (= Y152), K163 (= K156), P189 (= P182), G190 (= G183), M191 (≠ I184), I192 (≠ V185), T194 (= T187), G196 (≠ M189), T197 (≠ W190)
binding (2R)-2-(hydroxymethyl)pentanedioic acid: S146 (= S139), Y159 (= Y152), M191 (≠ I184), I202 (≠ R195)

7tzpG Crystal structure of putataive short-chain dehydrogenase/reductase (fabg) from klebsiella pneumoniae subsp. Pneumoniae ntuh-k2044 in complex with nadh (see paper)
37% identity, 99% coverage: 3:255/256 of query aligns to 9:247/247 of 7tzpG

query
sites
7tzpG

K

K

V

T

A

A

L

I

V

V

T

T

G
|
G

A

A

G

A