SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_007703655.1 NCBI__GCF_001277175.1:WP_007703655.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

2j6hA E. Coli glucosamine-6-p synthase in complex with glucose-6p and 5-oxo- l-norleucine (see paper)
93% identity, 100% coverage: 2:609/609 of query aligns to 1:608/608 of 2j6hA

query
sites
2j6hA

C
|
C

G

G

I

I

V

V

G

G

A

A

V

I

A

A

Q

Q

R

R

D

D

I

V

A

A

E

E

I

I

L

L

L

L

E

E

G

G

L

L

R

R

R

R

L

L

E

E

Y

Y

R
|
R

G
|
G

Y

Y

D

D

S

S

A

A

G

G

L

L

A

A

V

V

V

V

D

D

N

A

A

E

G

G

H

H

M

M

T

T

R

R

L

L

R

R

R

R

L

L

G

G

K

K

V

V

Q

Q

M

M

L

L

V

A

Q

Q

A

A

A

A

E

E

E

E

H

H

P

P

L

L

H

H

G

G

G

G

T

T

G

G

I

I

A

A

H

H

T

T

R
|
R

W
|
W

A

A

T
|
T

H

H

G

G

E

E

P

P

S

S

E

E

G

V

N

N

A

A

H
|
H

P

P

H

H

V

V

S

S

G

E

H

H

I

I

V

V

V

V

V

V

H

H

N
|
N

G
|
G

I

I

I

I

E

E

N

N

H

H

E

E

P

P

L

L

R

R

E

E

L

E

L

L

K

K

T

A

R

R

G

G

Y

Y

E

T

F

F

V

V

T

S

E

E

T

T

D
|
D

T

T

E

E

V

V

I

I

A

A

H

H

L

L

V

V

H

N

W

W

E

E

L

L

E

K

Q

Q

G

G

G

G

T

T

L

L

R

R

E

E

A

A

V

V

L

L

R

R

A

A

I

I

P

P

Q

Q

L

L

R

R

G

G

A

A

Y

Y

G

G

T

T

V

V

I

I

M

M

D

D

N

S

R

R

D

H

P

P

S

D

V

T

L

L

L

L

A

A

A

A

R

R

S

S

G

G

S

S

P

P

L

L

V

V

I

I

G

G

L

L

G

G

V

M

G

G

E

E

N

N

F

F

I

I

A

A

S

S

D

D

Q

Q

L

L

A

A

L

L

L

L

P

P

V

V

T

T

R

R

R

R

F

F

I

I

F

F

L

L

E

E

E

E

G

G

D

D

I

I

A

A

E

E

V

I

T

T

R

R

R

R

S

S

V

V

A

N

I

I

F

F

N

D

T

K

Q

T

G

G

E

A

S

E

V

V

S

K

R

R

P

Q

E

D

I

I

E

E

S

S

N

N

V

L

Q

Q

Y

Y

D

D

A

A

G

G

D

D

K

K

G

G

I

I

Y
|
Y

R

R

H

H

Y

Y

M

M

Q

Q

K

K

E

E

I

I

Y

Y

E

E

Q

Q

P

P

N

N

A

A

I

I

K

K

N

N

T

T

L

L

A

T

G

G

R

R

I

I

S

S

H

H

G

G

E

Q

V

V

D

D

L

L

S

S

E

E

L

L

G

G

P

P

N

N

A

A

D

D

E

E

L

L

L

L

S

S

Q

K

V

V

E

E

H

H

I

I

Q

Q

I

I

V

L

A

A

C

C

G

G

T
|
T

S

S

Y

Y

N

N

S

S

G

G

M

M

V

V

S

S

R

R

Y

Y

W

W

F

F

E

E

A

S

L

L

A

A

G

G

V

I

P

P

C

C

D

D

V

V

E

E

I

I

A

A

S

S

E

E

F

F

R

R

Y

Y

R

R

K

K

S

S

A

A

V

V

R

R

R

R

N

N

S

S

L

L

M

M

I

I

T

T

L

L

S
|
S

Q
|
Q

S
|
S

G

G

E

E

T
|
T

A

A

D

D

T

T

L

L

A

A

A

G

L

L

R

R

L

L

S

S

K

K

E

E

L

L

G

G

Y

Y

L

L

G

G

S

S

L

L

A

A

I

I

C

C

N

N

V

V

P

P

G

G

S

S

S

S

L

L

V

V

R

R

E

E

S

S

S

D

L

L

A

A

L

L

M

M

T

T

K

N

A

A

G

G

T

T

E

E

I

I

G

G

V
|
V

A

A

S
|
S

T

T

K

K

A

A

F

F

T

T

T

T

Q

Q

L

L

T

T

V

V

L

L

L

L

M

M

L

L

V

V

A

A

K

K

L

L

S

S

R

R

L

L

K

K

G

G

A

L

D

D

A

A

A

S

I

I

E

E

H

H

D

D

I

I

V

V

H

H

G

G

L

L

Q

Q

A

A

L

L

P

P

S

S

R

R

I

I

E

E

Q

Q

M

M

L

L

S

S

Q

Q

D

D

K

K

R

R

I

I

E

E

A

A

L

L

A

A

E

E

D

D

F

F

S

S

D

D

K

K

H

H

H

H

A

A

L

L

F

F

L

L

G

G

R

R

G

G

D

D

Q

Q

Y

Y

P

P

I

I

A

A

L

L

E
|
E

G

G

A

A

L

L

K
|
K

L

L

K

K

E
|
E

I

I

S

S

Y

Y

I

I

H

H

A

A

E

E

A

A

Y

Y

A

A

A

A

G

G

E

E

L

L

K

K

H
|
H

G

G

P

P

L

L

A

A

L

L

I

I

D

D

A

A

D

D

M

M

P

P

V

V

I

I

V

V

V

V

A

A

P

P

N

N

N

N

E

E

L

L

L

L

E

E

K

K

L

L

K

K

S

S

N

N

I

I

E

E

E

E

V

V

R

R

A

A

R

R

G

G

G

G

V

Q

L

L

Y

Y

V

V

F

F

A

A

D

D

Q

Q

D

D

A

A

G

G

F

F

S

V

S

S

S

S

D

D

N

N

M

M

N

H

I

I

I

I

P

E

M

M

P

P

H

H

V

V

E

E

E

E

V

V

I

I

A

A

P

P

I

I

F

F

Y

Y

T

T

V

V

P

P

L

L

Q

Q

L

L

L

L

A

A

Y

Y

H

H

V

V

A

A

L

L

I

I

K

K

G

G

T

T

D

D

V

V

D

D

Q

Q

P

P

R

R

N

N

L

L

A

A

K
|
K

S

S

V

V

T

T

V

V

E

E

active site: C1 (= C2), R26 (= R27), G27 (= G28), W74 (= W75), N98 (= N99), G99 (= G100), Y248 (= Y249), E481 (= E482), K485 (= K486), E488 (= E489), H504 (= H505), K603 (= K604)
binding glucose-6-phosphate: T302 (= T303), S347 (= S348), Q348 (= Q349), S349 (= S350), T352 (= T353), V399 (= V400), S401 (= S402), E488 (= E489)
binding 5-oxo-l-norleucine: C1 (= C2), R73 (= R74), W74 (= W75), T76 (= T77), H86 (= H87), N98 (= N99), G99 (= G100), D123 (= D124)

4amvA E.Coli glucosamine-6p synthase in complex with fructose-6p (see paper)
93% identity, 100% coverage: 2:609/609 of query aligns to 1:608/608 of 4amvA

query
sites
4amvA

C
|
C

G

G

I

I

V

V

G

G

A

A

V

I

A

A

Q

Q

R

R

D

D

I

V

A

A

E

E

I

I

L

L

L

L

E

E

G

G

L

L

R

R

R

R

L

L

E

E

Y

Y

R
|
R

G
|
G

Y

Y

D

D

S

S

A

A

G

G

L

L

A

A

V

V

V

V

D

D

N

A

A

E

G

G

H

H

M

M

T

T

R

R

L

L

R

R

R

R

L

L

G

G

K

K

V

V

Q

Q

M

M

L

L

V

A

Q

Q

A

A

A

A

E

E

E

E

H

H

P

P

L

L

H

H

G

G

G

G

T

T

G

G

I

I

A

A

H

H

T

T

R

R

W
|
W

A

A

T

T

H

H

G

G

E

E

P

P

S

S

E

E

G

V

N

N

A

A

H

H

P

P

H

H

V

V

S

S

G

E

H

H

I

I

V

V

V

V

V

V

H

H

N
|
N

G
|
G

I

I

I

I

E

E

N

N

H

H

E

E

P

P

L

L

R

R

E

E

L

E

L

L

K

K

T

A

R

R

G

G

Y

Y

E

T

F

F

V

V

T

S

E

E

T

T

D

D

T

T

E

E

V

V

I

I

A

A

H

H

L

L

V

V

H

N

W

W

E

E

L

L

E

K

Q

Q

G

G

G

G

T

T

L

L

R

R

E

E

A

A

V

V

L

L

R

R

A

A

I

I

P

P

Q

Q

L

L

R

R

G

G

A

A

Y

Y

G

G

T

T

V

V

I

I

M

M

D

D

N

S

R

R

D

H

P

P

S

D

V

T

L

L

L

L

A

A

A

A

R

R

S

S

G

G

S

S

P

P

L

L

V

V

I

I

G

G

L

L

G

G

V

M

G

G

E

E

N

N

F

F

I

I

A

A

S

S

D

D

Q

Q

L

L

A

A

L

L

L

L

P

P

V

V

T

T

R

R

R

R

F

F

I

I

F

F

L

L

E

E

E

E

G

G

D

D

I

I

A

A

E

E

V

I

T

T

R

R

R

R

S

S

V

V

A

N

I

I

F

F

N

D

T

K

Q

T

G

G

E

A

S

E

V

V

S

K

R

R

P

Q

E

D

I

I

E

E

S

S

N

N

V

L

Q

Q

Y

Y

D

D

A

A

G

G

D

D

K

K

G

G

I

I

Y
|
Y

R

R

H

H

Y

Y

M

M

Q

Q

K

K

E

E

I

I

Y

Y

E

E

Q

Q

P

P

N

N

A

A

I

I

K

K

N

N

T

T

L

L

A

T

G

G

R

R

I

I

S

S

H

H

G

G

E

Q

V

V

D

D

L

L

S

S

E

E

L

L

G

G

P

P

N

N

A

A

D

D

E

E

L

L

L

L

S

S

Q

K

V

V

E

E

H

H

I

I

Q

Q

I

I

V

L

A

A

C

C

G
|
G

T
|
T

S
|
S

Y

Y

N

N

S

S

G

G

M

M

V

V

S

S

R

R

Y

Y

W

W

F

F

E

E

A

S

L

L

A

A

G

G

V

I

P

P

C

C

D

D

V

V

E

E

I

I

A

A

S

S

E

E

F

F

R

R

Y

Y

R

R

K

K

S

S

A

A

V

V

R

R

R

R

N

N

S

S

L

L

M

M

I

I

T

T

L

L

S
|
S

Q
|
Q

S
|
S

G

G

E

E

T
|
T

A

A

D

D

T

T

L

L

A

A

A

G

L

L

R

R

L

L

S

S

K

K

E

E

L

L

G

G

Y

Y

L

L

G

G

S

S

L

L

A

A

I

I

C

C

N

N

V

V

P

P

G

G

S

S

S

S

L

L

V

V

R

R

E

E

S

S

S

D

L

L

A

A

L

L

M

M

T

T

K

N

A

A

G

G

T

T

E

E

I

I

G

G

V

V

A

A

S
|
S

T

T

K

K

A

A

F

F

T

T

T

T

Q

Q

L

L

T

T

V

V

L

L

L

L

M

M

L

L

V

V

A

A

K

K

L

L

S

S

R

K

L

L

K

K

G

G

A

L

D

D

A

A

A

S

I

I

E

E

H

H

D

D

I

I

V

V

H

H

G

G

L

L

Q

Q

A

A

L

L

P

P

S

S

R

R

I

I

E

E

Q

Q

M

M

L

L

S

S

Q

Q

D

D

K

K

R

R

I

I

E

E

A

A

L

L

A

A

E

E

D

D

F

F

S

S

D

D

K

K

H

H

H

H

A

A

L

L

F

F

L

L

G

G

R

R

G

G

D

D

Q

Q

Y

Y

P

P

I

I

A

A

L

L

E
|
E

G

G

A

A

L

L

K
|
K

L

L

K

K

E
|
E

I

I

S

S

Y

Y

I

I

H

H

A

A

E

E

A

A

Y

Y

A

A

A

A

G

G

E

E

L

L

K

K

H
|
H

G

G

P

P

L

L

A

A

L

L

I

I

D

D

A

A

D

D

M

M

P

P

V

V

I

I

V

V

V

V

A

A

P

P

N

N

N

N

E

E

L

L

L

L

E

E

K

K

L

L

K

K

S

S

N

N

I

I

E

E

E

E

V

V

R

R

A

A

R

R

G

G

G

G

V

Q

L

L

Y

Y

V

V

F

F

A

A

D

D

Q

Q

D

D

A

A

G

G

F

F

S

V

S

S

S

S

D

D

N

N

M

M

N

H

I

I

I

I

P

E

M

M

P

P

H

H

V

V

E

E

E

E

V

V

I

I

A

A

P

P

I

I

F

F

Y

Y

T

T

V

V

P

P

L

L

Q

Q

L

L

L

L

A

A

Y

Y

H

H

V

V

A

A

L

L

I

I

K

K

G

G

T

T

D

D

V

V

D

D

Q

Q

P

P

R

R

N

N

L

L

A

A

K
|
K

S

S

V

V

T

T

V

V

E

E

active site: C1 (= C2), R26 (= R27), G27 (= G28), W74 (= W75), N98 (= N99), G99 (= G100), Y248 (= Y249), E481 (= E482), K485 (= K486), E488 (= E489), H504 (= H505), K603 (= K604)
binding fructose -6-phosphate: G301 (= G302), T302 (= T303), S303 (= S304), S347 (= S348), Q348 (= Q349), S349 (= S350), T352 (= T353), S401 (= S402), K485 (= K486), E488 (= E489)

1jxaA Glucosamine 6-phosphate synthase with glucose 6-phosphate (see paper)
93% identity, 100% coverage: 2:609/609 of query aligns to 1:608/608 of 1jxaA

query
sites
1jxaA

C
|
C

G

G

I

I

V

V

G

G

A

A

V

I

A

A

Q

Q

R

R

D

D

I

V

A

A

E

E

I

I

L

L

L

L

E

E

G

G

L

L

R

R

R

R

L

L

E

E

Y

Y

R
|
R

G
|
G

Y

Y

D

D

S

S

A

A

G

G

L

L

A

A

V

V

V

V

D

D

N

A

A

E

G

G

H

H

M

M

T

T

R

R

L

L

R

R

R

R

L

L

G

G

K

K

V

V

Q

Q

M

M

L

L

V

A

Q

Q

A

A

A

A

E

E

E

E

H

H

P

P

L

L

H

H

G

G

G

G

T

T

G

G

I

I

A

A

H

H

T

T

R

R

W
|
W

A

A

T

T

H

H

G

G

E

E

P

P

S

S

E

E

G

V

N

N

A

A

H

H

P

P

H

H

V

V

S

S

G

E

H

H

I

I

V

V

V

V

V

V

H

H

N
|
N

G
|
G

I

I

I

I

E

E

N

N

H

H

E

E

P

P

L

L

R

R

E

E

L

E

L

L

K

K

T

A

R

R

G

G

Y

Y

E

T

F

F

V

V

T

S

E

E

T

T

D

D

T

T

E

E

V

V

I

I

A

A

H

H

L

L

V

V

H

N

W

W

E

E

L

L

E

K

Q

Q

G

G

G

G

T

T

L

L

R

R

E

E

A

A

V

V

L

L

R

R

A

A

I

I

P

P

Q

Q

L

L

R

R

G

G

A

A

Y

Y

G

G

T

T

V

V

I

I

M

M

D

D

N

S

R

R

D

H

P

P

S

D

V

T

L

L

L

L

A

A

A

A

R

R

S

S

G

G

S

S

P

P

L

L

V

V

I

I

G

G

L

L

G

G

V

M

G

G

E

E

N

N

F

F

I

I

A

A

S

S

D

D

Q

Q

L

L

A

A

L

L

L

L

P

P

V

V

T

T

R

R

R

R

F

F

I

I

F

F

L

L

E

E

E

E

G

G

D

D

I

I

A

A

E

E

V

I

T

T

R

R

R

R

S

S

V

V

A

N

I

I

F

F

N

D

T

K

Q

T

G

G

E

A

S

E

V

V

S

K

R

R

P

Q

E

D

I

I

E

E

S

S

N

N

V

L

Q

Q

Y

Y

D

D

A

A

G

G

D

D

K

K

G

G

I

I

Y
|
Y

R

R

H

H

Y

Y

M

M

Q

Q

K

K

E

E

I

I

Y

Y

E

E

Q

Q

P

P

N

N

A

A

I

I

K

K

N

N

T

T

L

L

A

T

G

G

R

R

I

I

S

S

H

H

G

G

E

Q

V

V

D

D

L

L

S

S

E

E

L

L

G

G

P

P

N

N

A

A

D

D

E

E

L

L

L

L

S

S

Q

K

V

V

E

E

H

H

I

I

Q

Q

I

I

V

L

A

A

C

C

G

G

T
|
T

S
|
S

Y

Y

N

N

S

S

G

G

M

M

V

V

S

S

R

R

Y

Y

W

W

F

F

E

E

A

S

L

L

A

A

G

G

V

I

P

P

C

C

D

D

V

V

E

E

I

I

A

A

S

S

E

E

F

F

R

R

Y

Y

R

R

K

K

S

S

A

A

V

V

R

R

R

R

N

N

S

S

L

L

M

M

I

I

T

T

L

L

S
|
S

Q
|
Q

S
|
S

G

G

E

E

T
|
T

A

A

D

D

T

T

L

L

A

A

A

G

L

L

R

R

L

L

S

S

K

K

E

E

L

L

G

G

Y

Y

L

L

G

G

S

S

L

L

A

A

I

I

C

C

N

N

V

V

P

P

G

G

S

S

S

S

L

L

V

V

R

R

E

E

S

S

S

D

L

L

A

A

L

L

M

M

T

T

K

N

A

A

G

G

T

T

E

E

I

I

G

G

V

V

A

A

S
|
S

T

T

K

K

A

A

F

F

T

T

T

T

Q

Q

L

L

T

T

V

V

L

L

L

L

M

M

L

L

V

V

A

A

K

K

L

L

S

S

R

K

L

L

K

K

G

G

A

L

D

D

A

A

A

S

I

I

E

E

H

H

D

D

I

I

V

V

H

H

G

G

L

L

Q

Q

A

A

L

L

P

P

S

S

R

R

I

I

E

E

Q

Q

M

M

L

L

S

S

Q

Q

D

D

K

K

R

R

I

I

E

E

A

A

L

L

A

A

E

E

D

D

F

F

S

S

D

D

K

K

H

H

H

H

A

A

L

L

F

F

L

L

G

G

R

R

G

G

D

D

Q

Q

Y

Y

P

P

I

I

A

A

L

L

E
|
E

G

G

A

A

L

L

K
|
K

L

L

K

K

E
|
E

I

I

S

S

Y

Y

I

I

H

H

A

A

E

E

A

A

Y

Y

A

A

A

A

G

G

E

E

L

L

K

K

H
|
H

G

G

P

P

L

L

A

A

L

L

I

I

D

D

A

A

D

D

M

M

P

P

V

V

I

I

V

V

V

V

A

A

P

P

N

N

N

N

E

E

L

L

L

L

E

E

K

K

L

L

K

K

S

S

N

N

I

I

E

E

E

E

V

V

R

R

A

A

R

R

G

G

G

G

V

Q

L

L

Y

Y

V

V

F

F

A

A

D

D

Q

Q

D

D

A

A

G

G

F

F

S

V

S

S

S

S

D

D

N

N

M

M

N

H

I

I

I

I

P

E

M

M

P

P

H

H

V

V

E

E

E

E

V

V

I

I

A

A

P

P

I

I

F

F

Y

Y

T

T

V

V

P

P

L

L

Q

Q

L

L

L

L

A

A

Y

Y

H

H

V

V

A

A

L

L

I

I

K

K

G

G

T

T

D

D

V

V

D

D

Q

Q

P

P

R

R

N

N

L

L

A

A

K
|
K

S

S

V

V

T

T

V

V

E

E

active site: C1 (= C2), R26 (= R27), G27 (= G28), W74 (= W75), N98 (= N99), G99 (= G100), Y248 (= Y249), E481 (= E482), K485 (= K486), E488 (= E489), H504 (= H505), K603 (= K604)
binding glucose-6-phosphate: T302 (= T303), S303 (= S304), S347 (= S348), Q348 (= Q349), S349 (= S350), T352 (= T353), S401 (= S402), K485 (= K486), E488 (= E489)

1mosA Isomerase domain of glucosamine 6-phosphate synthase complexed with 2- amino-2-deoxyglucitol 6-phosphate (see paper)
96% identity, 60% coverage: 243:609/609 of query aligns to 1:367/367 of 1mosA

query
sites
1mosA

A

A

G

G

D

D

K

K

G

G

I

I

Y

Y

R

R

H

H

Y

Y

M

M

Q

Q

K

K

E

E

I

I

Y

Y

E

E

Q

Q

P

P

N

N

A

A

I

I

K

K

N

N

T

T

L

L

A

T

G

G

R

R

I

I

S

S

H

H

G

G

E

Q

V

V

D

D

L

L

S

S

E

E

L

L

G

G

P

P

N

N

A

A

D

D

E

E

L

L

L

L

S

S

Q

K

V

V

E

E

H

H

I

I

Q

Q

I

I

V

L

A

A

C

C

G

G

T
|
T

S
|
S

Y

Y

N

N

S

S

G

G

M

M

V

V

S

S

R

R

Y

Y

W

W

F

F

E

E

A

S

L

L

A

A

G

G

V

I

P

P

C

C

D

D

V

V

E

E

I

I

A

A

S

S

E

E

F

F

R

R

Y

Y

R

R

K

K

S

S

A

A

V

V

R

R

R

R

N

N

S

S

L

L

M

M

I

I

T

T

L

L

S
|
S

Q
|
Q

S
|
S

G

G

E

E

T
|
T

A

A

D

D

T

T

L

L

A

A

A

G

L

L

R

R

L

L

S

S

K

K

E

E

L

L

G

G

Y

Y

L

L

G

G

S

S

L

L

A

A

I

I

C

C

N

N

V

V

P

P

G

G

S

S

S

S

L

L

V

V

R

R

E

E

S

S

S

D

L

L

A

A

L

L

M

M

T

T

K

N

A

A

G

G

T

T

E

E

I

I

G

G

V

V

A

A

S

S

T

T

K

K

A

A

F

F

T

T

T

T

Q

Q

L

L

T

T

V

V

L

L

L

L

M

M

L

L

V

V

A

A

K

K

L

L

S

S

R

R

L

L

K

K

G

G

A

L

D

D

A

A

A

S

I

I

E

E

H

H

D

D

I

I

V

V

H

H

G

G

L

L

Q

Q

A

A

L

L

P

P

S

S

R

R

I

I

E

E

Q

Q

M

M

L

L

S

S

Q

Q

D

D

K

K

R

R

I

I

E

E

A

A

L

L

A

A

E

E

D

D

F

F

S

S

D

D

K

K

H

H

H

H

A

A

L

L

F

F

L

L

G

G

R

R

G

G

D

D

Q

Q

Y

Y

P

P

I

I

A

A

L

L

E
|
E

G

G

A

A

L

L

K
|
K

L

L

K

K

E
|
E

I

I

S

S

Y

Y

I

I

H

H

A

A

E

E

A

A

Y

Y

A

A

A

A

G

G

E

E

L

L

K

K

H
|
H

G

G

P

P

L

L

A

A

L

L

I

I

D

D

A

A

D

D

M

M

P

P

V

V

I

I

V

V

V

V

A

A

P

P

N

N

N

N

E

E

L

L

L

L

E

E

K

K

L

L

K

K

S

S

N

N

I

I

E

E

E

E

V

V

R

R

A

A

R

R

G

G

G

G

V

Q

L

L

Y

Y

V

V

F

F

A

A

D

D

Q

Q

D

D

A

A

G

G

F

F

S

V

S

S

S

S

D

D

N

N

M

M

N

H

I

I

I

I

P

E

M

M

P

P

H

H

V

V

E

E

E

E

V

V

I

I

A

A

P

P

I

I

F

F

Y

Y

T

T

V

V

P

P

L

L

Q

Q

L

L

L

L

A

A

Y

Y

H

H

V

V

A

A

L

L

I

I

K

K

G

G

T

T

D

D

V

V

D

D

Q

Q

P

P

R

R

N

N

L

L

A

A

K
|
K

S

S

V

V

T

T

V

V

E

E

active site: E240 (= E482), K244 (= K486), E247 (= E489), H263 (= H505), K362 (= K604)
binding 2-deoxy-2-amino glucitol-6-phosphate: T61 (= T303), S62 (= S304), S106 (= S348), Q107 (= Q349), S108 (= S350), T111 (= T353), K244 (= K486), E247 (= E489)

1morA Isomerase domain of glucosamine 6-phosphate synthase complexed with glucose 6-phosphate (see paper)
96% identity, 60% coverage: 244:609/609 of query aligns to 1:366/366 of 1morA

query
sites
1morA

G

G

D

D

K

K

G

G

I

I

Y

Y

R

R

H

H

Y

Y

M

M

Q

Q

K

K

E

E

I

I

Y

Y

E

E

Q

Q

P

P

N

N

A

A

I

I

K

K

N

N

T

T

L

L

A

T

G

G

R

R

I

I

S

S

H

H

G

G

E

Q

V

V

D

D

L

L

S

S

E

E

L

L

G

G

P

P

N

N

A

A

D

D

E

E

L

L

L

L

S

S

Q

K

V

V

E

E

H

H

I

I

Q

Q

I

I

V

L

A

A

C

C

G

G

T
|
T

S

S

Y

Y

N

N

S

S

G

G

M

M

V

V

S

S

R

R

Y

Y

W

W

F

F

E

E

A

S

L

L

A

A

G

G

V

I

P

P

C

C

D

D

V

V

E

E

I

I

A

A

S

S

E

E

F

F

R

R

Y

Y

R

R

K

K

S

S

A

A

V

V

R

R

R

R

N

N

S

S

L

L

M

M

I

I

T

T

L

L

S
|
S

Q
|
Q

S
|
S

G

G

E

E

T
|
T

A

A

D

D

T

T

L

L

A

A

A

G

L

L

R

R

L

L

S

S

K

K

E

E

L

L

G

G

Y

Y

L

L

G

G

S

S

L

L

A

A

I

I

C

C

N

N

V

V

P

P

G

G

S

S

S

S

L

L

V

V

R

R

E

E

S

S

S

D

L

L

A

A

L

L

M

M

T

T

K

N

A

A

G

G

T

T

E

E

I

I

G

G

V
|
V

A

A

S

S

T

T

K

K

A

A

F

F

T

T

T

T

Q

Q

L

L

T

T

V

V

L

L

L

L

M

M

L

L

V

V

A

A

K

K

L

L

S

S

R

R

L

L

K

K

G

G

A

L

D

D

A

A

A

S

I

I

E

E

H

H

D

D

I

I

V

V

H

H

G

G

L

L

Q

Q

A

A

L

L

P

P

S

S

R

R

I

I

E

E

Q

Q

M

M

L

L

S

S

Q

Q

D

D

K

K

R

R

I

I

E

E

A

A

L

L

A

A

E

E

D

D

F

F

S

S

D

D

K

K

H

H

H

H

A

A

L

L

F

F

L

L

G

G

R

R

G

G

D

D

Q

Q

Y

Y

P

P

I

I

A

A

L

L

E
|
E

G

G

A

A

L

L

K
|
K

L

L

K

K

E
|
E

I

I

S

S

Y

Y

I

I

H

H

A

A

E

E

A

A

Y

Y

A

A

A

A

G

G

E

E

L

L

K

K

H
|
H

G

G

P

P

L

L

A

A

L

L

I

I

D

D

A

A

D

D

M

M

P

P

V

V

I

I

V

V

V

V

A

A

P

P

N

N

N

N

E

E

L

L

L

L

E

E

K

K

L

L

K

K

S

S

N

N

I

I

E

E

E

E

V

V

R

R

A

A

R

R

G

G

G

G

V

Q

L

L

Y

Y

V

V

F

F

A

A

D

D

Q

Q

D

D

A

A

G

G

F

F

S

V

S

S

S

S

D

D

N

N

M

M

N

H

I

I

I

I

P

E

M

M

P

P

H

H

V

V

E

E

E

E

V

V

I

I

A

A

P

P

I

I

F

F

Y

Y

T

T

V

V

P

P

L

L

Q

Q

L

L

L

L

A

A

Y

Y

H

H

V

V

A

A

L

L

I

I

K

K

G

G

T

T

D

D

V

V

D

D

Q

Q

P

P

R

R

N

N

L

L

A
|
A

K
|
K

S

S

V

V

T

T

V

V

E

E

active site: E239 (= E482), K243 (= K486), E246 (= E489), H262 (= H505), K361 (= K604)
binding 6-O-phosphono-alpha-D-glucopyranose: T60 (= T303), S105 (= S348), Q106 (= Q349), S107 (= S350), T110 (= T353), V157 (= V400), A360 (= A603), K361 (= K604)

1moqA Isomerase domain of glucosamine 6-phosphate synthase complexed with glucosamine 6-phosphate (see paper)
96% identity, 60% coverage: 244:609/609 of query aligns to 1:366/366 of 1moqA

query
sites
1moqA

G

G

D

D

K

K

G

G

I

I

Y

Y

R

R

H

H

Y

Y

M

M

Q

Q

K

K

E

E

I

I

Y

Y

E

E

Q

Q

P

P

N

N

A

A

I

I

K

K

N

N

T

T

L

L

A

T

G

G

R

R

I

I

S

S

H

H

G

G

E

Q

V

V

D

D

L

L

S

S

E

E

L

L

G

G

P

P

N

N

A

A

D

D

E

E

L

L

L

L

S

S

Q

K

V

V

E

E

H

H

I

I

Q

Q

I

I

V

L

A

A

C

C

G

G

T
|
T

S
|
S

Y

Y

N

N

S

S

G

G

M

M

V

V

S

S

R

R

Y

Y

W

W

F

F

E

E

A

S

L

L

A

A

G

G

V

I

P

P

C

C

D

D

V

V

E

E

I

I

A

A

S

S

E

E

F

F

R

R

Y

Y

R

R

K

K

S

S

A

A

V

V

R

R

R

R

N

N

S

S

L

L

M

M

I

I

T

T

L

L

S
|
S

Q
|
Q

S
|
S

G

G

E

E

T
|
T

A

A

D

D

T

T

L

L

A

A

A

G

L

L

R

R

L

L

S

S

K

K

E

E

L

L

G

G

Y

Y

L

L

G

G

S

S

L

L

A

A

I

I

C

C

N

N

V

V

P

P

G

G

S

S

S

S

L

L

V

V

R

R

E

E

S

S

S

D

L

L

A

A

L

L

M

M

T

T

K

N

A

A

G

G

T

T

E

E

I

I

G

G

V
|
V

A

A

S

S

T

T

K

K

A

A

F

F

T

T

T

T

Q

Q

L

L

T

T

V

V

L

L

L

L

M

M

L

L

V

V

A

A

K

K

L

L

S

S

R

R

L

L

K

K

G

G

A

L

D

D

A

A

A

S

I

I

E

E

H

H

D

D

I

I

V

V

H

H

G

G

L

L

Q

Q

A

A

L

L

P

P

S

S

R

R

I

I

E

E

Q

Q

M

M

L

L

S

S

Q

Q

D

D

K

K

R

R

I

I

E

E

A

A

L

L

A

A

E

E

D

D

F

F

S

S

D

D

K

K

H

H

H

H

A

A

L

L

F

F

L

L

G

G

R

R

G

G

D

D

Q

Q

Y

Y

P

P

I

I

A

A

L

L

E
|
E

G

G

A

A

L

L

K
|
K

L

L

K

K

E
|
E

I

I

S

S

Y

Y

I

I

H

H

A

A

E

E

A

A

Y

Y

A

A

A

A

G

G

E

E

L

L

K

K

H
|
H

G

G

P

P

L

L

A

A

L

L

I

I

D

D

A

A

D

D

M

M

P

P

V

V

I

I

V

V

V

V

A

A

P

P

N

N

N

N

E

E

L

L

L

L

E

E

K

K

L

L

K

K

S

S

N

N

I

I

E

E

E

E

V

V

R

R

A

A

R

R

G

G

G

G

V

Q

L

L

Y

Y

V

V

F

F

A

A

D

D

Q

Q

D

D

A

A

G

G

F

F

S

V

S

S

S

S

D

D

N

N

M

M

N

H

I

I

I

I

P

E

M

M

P

P

H

H

V

V

E

E

E

E

V

V

I

I

A

A

P

P

I

I

F

F

Y

Y

T

T

V

V

P

P

L

L

Q

Q

L

L

L

L

A

A

Y

Y

H

H

V

V

A

A

L

L

I

I

K

K

G

G

T

T

D

D

V

V

D

D

Q

Q

P

P

R

R

N

N

L

L

A
|
A

K
|
K

S

S

V

V

T

T

V

V

E

E

active site: E239 (= E482), K243 (= K486), E246 (= E489), H262 (= H505), K361 (= K604)
binding 2-amino-2-deoxy-6-O-phosphono-alpha-D-glucopyranose: T60 (= T303), S61 (= S304), S105 (= S348), Q106 (= Q349), S107 (= S350), T110 (= T353), V157 (= V400), A360 (= A603), K361 (= K604)

7dnrA Crystal structure of zn-bound sis domain of glucosamine-6-p synthase from e. Coli
96% identity, 59% coverage: 244:600/609 of query aligns to 1:357/357 of 7dnrA

query
sites
7dnrA

G

G

D

D

K

K

G

G

I

I

Y

Y

R

R

H

H

Y

Y

M

M

Q

Q

K

K

E

E

I

I

Y

Y

E

E

Q

Q

P

P

N

N

A

A

I

I

K

K

N

N

T

T

L

L

A

T

G

G

R

R

I

I

S

S

H

H

G

G

E

Q

V

V

D

D

L

L

S

S

E

E

L

L

G

G

P

P

N

N

A

A

D

D

E

E

L

L

L

L

S

S

Q

K

V

V

E

E

H

H

I

I

Q

Q

I

I

V

L

A

A

C

C

G

G

T

T

S

S

Y

Y

N

N

S

S

G

G

M

M

V

V

S

S

R

R

Y

Y

W

W

F

F

E

E

A

S

L

L

A

A

G

G

V

I

P

P

C

C

D

D

V

V

E

E

I

I

A

A

S

S

E

E

F

F

R

R

Y

Y

R

R

K

K

S

S

A

A

V

V

R

R

R

R

N

N

S

S

L

L

M

M

I

I

T

T

L

L

S

S

Q

Q

S

S

G

G

E

E

T

T

A

A

D

D

T

T

L

L

A

A

A

G

L

L

R

R

L

L

S

S

K

K

E

E

L

L

G

G

Y

Y

L

L

G

G

S

S

L

L

A

A

I

I

C

C

N

N

V

V

P

P

G

G

S

S

S

S

L

L

V

V

R

R

E

E

S

S

S

D

L

L

A

A

L

L

M

M

T

T

K

N

A

A

G

G

T

T

E

E

I

I

G

G

V

V

A

A

S

S

T

T

K

K

A

A

F

F

T

T

T

T

Q

Q

L

L

T

T

V

V

L

L

L

L

M

M

L

L

V

V

A

A

K

K

L

L

S

S

R

R

L

L

K

K

G

G

A

L

D

D

A

A

A

S

I

I

E

E

H

H

D

D

I

I

V

V

H

H

G

G

L

L

Q

Q

A

A

L

L

P

P

S

S

R

R

I

I

E

E

Q

Q

M

M

L

L

S

S

Q

Q

D

D

K

K

R

R

I

I

E

E

A

A

L

L

A

A

E

E

D

D

F

F

S

S

D

D

K

K

H
|
H

H
|
H

A

A

L

L

F

F

L

L

G

G

R

R

G

G

D

D

Q

Q

Y

Y

P

P

I

I

A

A

L

L

E

E

G

G

A

A

L

L

K

K

L

L

K

K

E

E

I

I

S

S

Y

Y

I

I

H

H

A

A

E

E

A

A

Y

Y

A

A

A

A

G

G

E

E

L

L

K

K

H

H

G

G

P

P

L

L

A

A

L

L

I

I

D

D

A

A

D

D

M

M

P

P

V

V

I

I

V

V

V

V

A

A

P

P

N

N

N

N

E

E

L

L

L

L

E

E

K

K

L

L

K

K

S

S

N

N

I

I

E

E

E

E

V

V

R

R

A

A

R

R

G

G

G

G

V

Q

L

L

Y

Y

V

V

F

F

A

A

D

D

Q

Q

D

D

A

A

G

G

F

F

S

V

S

S

S

S

D

D

N

N

M

M

N

H

I

I

I

I

P

E

M

M

P

P

H

H

V

V

E

E

E

E

V

V

I

I

A

A

P

P

I

I

F

F

Y

Y

T

T

V

V

P

P

L

L

Q

Q

L

L

L

L

A

A

Y

Y

H

H

V

V

A

A

L

L

I

I

K

K

G

G

T

T

D

D

V

V

D

D

Q

Q

P

P

R

R

binding zinc ion: H223 (= H466), H224 (= H467)

1xfgA Glutaminase domain of glucosamine 6-phosphate synthase complexed with l-glu hydroxamate (see paper)
88% identity, 39% coverage: 2:239/609 of query aligns to 1:238/238 of 1xfgA

query
sites
1xfgA

C
|
C

G

G

I

I

V

V

G

G

A

A

V

I

A

A

Q

Q

R

R

D

D

I

V

A

A

E

E

I

I

L

L

L

L

E

E

G

G

L

L

R

R

R

R

L

L

E

E

Y

Y

R
|
R

G
|
G

Y

Y

D

D

S

S

A

A

G

G

L

L

A

A

V

V

V

V

D

D

N

A

A

E

G

G

H

H

M

M

T

T

R

R

L

L

R

R

R

R

L

L

G

G

K

K

V

V

Q

Q

M

M

L

L

V

A

Q

Q

A

A

A

A

E

E

E

E

H

H

P

P

L

L

H

H

G

G

G

G

T

T

G

G

I

I

A

A

H

H

T

T

R
|
R

W
|
W

A

A

T
|
T

H

H

G

G

E

E

P

P

S

S

E

E

G

V

N

N

A

A

H
|
H

P

P

H

H

V

V

S

S

G

E

H

H

I

I

V

V

V

V

V

V

H

H

N
|
N

G
|
G

I

I

I

I

E

E

N

N

H

H

E

E

P

P

L

L

R

R

E

E

L

E

L

L

K

K

T

A

R

R

G

G

Y

Y

E

T

F

F

V

V

T

S

E

E

T

T

D
|
D

T

T

E

E

V

V

I

I

A

A

H

H

L

L

V

V

H

N

W

W

E

E

L

L

E

K

Q

Q

G

G

G

G

T

T

L

L

R

R

E

E

A

A

V

V

L

L

R

R

A

A

I

I

P

P

Q

Q

L

L

R

R

G

G

A

A

Y

Y

G

G

T

T

V

V

I

I

M

M

D

D

N

S

R

R

D

H

P

P

S

D

V

T

L

L

L

L

A

A

A

A

R

R

S

S

G

G

S

S

P

P

L

L

V

V

I

I

G

G

L

L

G

G

V

M

G

G

E

E

N

N

F

F

I

I

A

A

S

S

D

D

Q

Q

L

L

A

A

L

L

L

L

P

P

V

V

T

T

R

R

R

R

F

F

I

I

F

F

L

L

E

E

E

E

G

G

D

D

I

I

A

A

E

E

V

I

T

T

R

R

R

R

S

S

V

V

A

N

I

I

F

F

N

D

T

K

Q

T

G

G

E

A

S

E

V

V

S

K

R

R

P

Q

E

D

I

I

E

E

S

S

N

N

V

L

active site: C1 (= C2), R26 (= R27), G27 (= G28), W74 (= W75), N98 (= N99), G99 (= G100)
binding glutamine hydroxamate: C1 (= C2), R73 (= R74), W74 (= W75), T76 (= T77), H86 (= H87), N98 (= N99), G99 (= G100), D123 (= D124)

1xffA Glutaminase domain of glucosamine 6-phosphate synthase complexed with glutamate (see paper)
88% identity, 39% coverage: 2:239/609 of query aligns to 1:238/238 of 1xffA

query
sites
1xffA

C
|
C

G

G

I

I

V

V

G

G

A

A

V

I

A

A

Q

Q

R

R

D

D

I

V

A

A

E

E

I

I

L

L

L

L

E

E

G

G

L

L

R

R

R

R

L

L

E

E

Y

Y

R
|
R

G
|
G

Y

Y

D

D

S

S

A

A

G

G

L

L

A

A

V

V

V

V

D

D

N

A

A

E

G

G

H

H

M

M

T

T

R

R

L

L

R

R

R

R

L

L

G

G

K

K

V

V

Q

Q

M

M

L

L

V

A

Q

Q

A

A

A

A

E

E

E

E

H

H

P

P

L

L

H

H

G

G

G

G

T

T

G

G

I

I

A

A

H

H

T

T

R
|
R

W
|
W

A

A

T
|
T

H

H

G

G

E

E

P

P

S

S

E

E

G

V

N

N

A

A

H
|
H

P

P

H

H

V

V

S

S

G

E

H

H

I

I

V

V

V

V

V

V

H

H

N
|
N

G
|
G

I

I

I

I

E

E

N

N

H

H

E

E

P

P

L

L

R

R

E

E

L

E

L

L

K

K

T

A

R

R

G

G

Y

Y

E

T

F

F

V

V

T

S

E

E

T

T

D
|
D

T

T

E

E

V

V

I

I

A

A

H

H

L

L

V

V

H

N

W

W

E

E

L

L

E

K

Q

Q

G

G

G

G

T

T

L

L

R

R

E

E

A

A

V

V

L

L

R

R

A

A

I

I

P

P

Q

Q

L

L

R

R

G

G

A

A

Y

Y

G

G

T

T

V

V

I

I

M

M

D

D

N

S

R

R

D

H

P

P

S

D

V

T

L

L

L

L

A

A

A

A

R

R

S

S

G

G

S

S

P

P

L

L

V

V

I

I

G

G

L

L

G

G

V

M

G

G

E

E

N

N

F

F

I

I

A

A

S

S

D

D

Q

Q

L

L

A

A

L

L

L

L

P

P

V

V

T

T

R

R

R

R

F

F

I

I

F

F

L

L

E

E

E

E

G

G

D

D

I

I

A

A

E

E

V

I

T

T

R

R

R

R

S

S

V

V

A

N

I

I

F

F

N

D

T

K

Q

T

G

G

E

A

S

E

V

V

S

K

R

R

P

Q

E

D

I

I

E

E

S

S

N

N

V

L

active site: C1 (= C2), R26 (= R27), G27 (= G28), W74 (= W75), N98 (= N99), G99 (= G100)
binding glutamic acid: C1 (= C2), R73 (= R74), W74 (= W75), T76 (= T77), H86 (= H87), N98 (= N99), G99 (= G100), D123 (= D124)

6svmA Crystal structure of human gfat-1 in complex with glucose-6-phosphate, l-glu, and udp-galnac (see paper)
39% identity, 100% coverage: 2:609/609 of query aligns to 1:660/660 of 6svmA

query
sites
6svmA

C
|
C

G

G

I

I

V

F

G

A

A

Y

V
x
L

-

N

-

Y

-

H

-

V

-

P

-

R

A

T

Q

R

R

R

D

E

I

I

A

L

E

E

I

T

L

L

L

I

E

K

G

G

L

L

R

Q

R

R

L

L

E

E

Y

Y

R
|
R

G

G

Y

Y

D

D

S

S

A

A

G

G

L

V

A

G

V

F

V

D

D

G

-

G

-

N

-

D

-

K

-

D

-

W

-

E

-

A

N

N

A

A

G

C

H

K

M

I

T

Q

R

L

L

I

R

K

R

K

L

K

G

G

K

K

V

V

Q

K

M

A

L

L

V

D

Q

E

A

E

A

V

E

H

-

K

-

Q

-

Q

-

D

-

M

-

D

-

L

-

D

-

I

E

E

H

F

P

D

L

V

H

H

G

-

G

-

T

L

G

G

I

I

A

A

H

H

T

T

R
|
R

W
|
W

A

A

T
|
T

H

H

G

G

E

E

P

P

S

S

E

P

G

V

N

N

A

S

H
|
H

P

P

H

Q

V

R

S

S

-

D

-

K

-

N

G

N

H

E

I

F

V

I

V

V

V

I

H

H

N
|
N

G
|
G

I

I

I

I

E

T

N

N

H

Y

E

K

P

D

L

L

R

K

E

K

L

F

L

L

K

E

T

S

R

K

G

G

Y

Y

E

D

F

F

V

E

T

S

E

E

T

T

D
|
D

T

T

E

E

V

T

I

I

A

A

H

K

L

L

V

V

H

K

W

Y

E

M

L

Y

E

D

-

N

-

R

-

E

-

S

Q

Q

G

D

G

T

T

S

L

F

R

T

E

T

A

L

V

V

L

E

R

R

A

V

I

I

P

Q

Q

Q

L

L

R

E

G

G

A

A

Y

F

G

A

T

L

V

V

I

F

M

K

D

S

N

V

R

H

D

F

P

P

S

G

V

Q

L

A

L

V

A

G

A

T

R

R

S

R

G

G

S

S

P

P

L

L

V

L

I

I

G

G

L

V

-

R

-

S

-

E

-

H

-

K

-

L

-

S

-

T

-

D

-

H

-

I

-

P

-

I

-

L

-

Y

-

R

-

S

-

T

-

T

-

C

-

L

-

F

-

P

-

V

-

E

-

E

G

K

V

A

G

V

E

E

N

Y

F

Y

I

F

A

A

S

S

D

D

Q

A

L

S

A

A

L

V

L

I

P

E

V

H

T

T

R

N

R

R

F

V

I

I

F

F

L

L

E

E

E

D

G

D

D

D

I

V

A

A

E

A

V

V

T

V

-

D

-

G

R

R

R

L

S

S

V

I

A

H

I

R

F

I

N

K

T

H

Q

P

G

G

E

R

S

A

V

V

S

Q

R

T

P

L

E
x
Q

I

M

E

E

S

L

N

Q

V

Q

Q

I

Y

M

D

-

A

-

G

-

D

-

K

K

G

G

I

N

Y

F

R

S

H

S

Y

F

M

M

Q

Q

K

K

E

E

I

I

Y

F

E

E

Q

Q

P

P

N

E

A

S

I

V

K

V

N

N

T

T

L

M

A

R

G

G

R
|
R

I

V

S

N

H

F

G

D

E

D

-

Y

-

T

V

V

D

N

L

L

S

G

E
x
G

L

L

G

K

P

D

N

H

A

I

D

K

E

E

L

I

L

-

S

Q

Q

R

V

C

E

R

H

R

I

L

Q

I

I

L

V

I

A

A

C
|
C

G

G

T
|
T

S
|
S

Y

Y

N

H

S

A

G

G

M

V

V

A

S

T

R

R

Y

Q

W

V

F

L

E

E

A

E

L

L

A

T

G

E

V

L

P

P

C

V

D

M

V

V

E

E

I

L

A

A

S

S

E

D

F

F

R

L

Y

D

R

R

K

N

S

T

A

P

V

V

R

F

R

R

N

D

S

D

L

V

M

C

I

F

T

F

L

L

S
|
S

Q
|
Q

S
|
S

G

G

E

E

T
|
T

A

A

D

D

T

T

L

L

A

M

A

G

L

L

R

R

L

Y

S

C

K

K

E

E

L

R

G

G

Y

A

L

L

G

-

S

T

L

V

A
x
G

I

I

C
x
T

N

N

V

T

P

V

G

G

S

S

S

S

L

I

V
x
S

R
|
R

E

E

S
x
T

S

D

L
x
C

A
x
G

L
x
V

M
x
H

T

I

K

N

A

A

G

G

T

P

E

E

I

I

G

G

V

V

A

A

S
|
S

T

T

K

K

A

A

F

Y

T

T

T

S

Q

Q

L

F

T

V

V

S

L

L

L

V

M

M

L

F

V

A

A

L

K

M

L

M

S

C

R

D

L

D

K

R

G

I

A

S

D

M

A

Q

A

E

I

R

E

R

H

K

D

E

I

I

V

M

H

L

G

G

L

L

Q

K

A

R

L

L

P

P

S

D

R

L

I

I

E

K

Q

E

M

V

L

L

S

S

Q

M

D

D

K

D

R

E

I

I

E

Q

A

K

L

L

A

A

E

T

D

E

F

L

S

Y

D

H

K

Q

H

K

H

S

A

V

L

L

F

I

L

M

G

G

R

R

G

G

D

Y

Q

H

Y

Y

P

A

I

T

A

C

L

L

E
|
E

G

G

A

A

L

L

K
|
K

L

I

K

K

E
|
E

I

I

S

T

Y

Y

I

M

H

H

A

S

E

E

A

G

Y

I

A

L

A

A

G

G

E

E

L

L

K

K

H
|
H

G

G

P

P

L

L

A

A

L

L

I

V

D

D

A

K

D

L

M

M

P

P

V

V

I

I

V

M

V

I

A

I

P

M

N

R

N

D

E

H

L

T

L

Y

E

A

K

K

L

C

K

Q

S

N

N

A

I

L

E

Q

E

Q

V

V

R

V

A

A

R

R

G

Q

G

G

V

R

L

P

Y

V

V

V

F

I

A

C

D

D

Q

K

D

E

A

D

G

T

F

E

S

T

S

I

S

K

D

N

N

T

M

K

N

R

I

T

I

I

P

K

M

V

P

P

H

H

V

S

E

V

E

D

V

C

I

L

A

Q

P

G

I

I

F

L

Y

S

T

V

V

I

P

P

L

L

Q

Q

L

L

L

L

A

A

Y

F

H

H

V

L

A

A

L

V

I

L

K

R

G

G

T

Y

D

D

V

V

D

D

Q

F

P

P

R

R

N

N

L

L

A

A

K
|
K

S

S

V

V

T

T

V

V

E

E

active site: L7 (≠ V8), R32 (= R27), W95 (= W75), N122 (= N99), G123 (= G100), E533 (= E482), K537 (= K486), E540 (= E489), H556 (= H505), K655 (= K604)
binding glucose-6-phosphate: C353 (= C301), T355 (= T303), S356 (= S304), S400 (= S348), Q401 (= Q349), S402 (= S350), T405 (= T353), S453 (= S402), K537 (= K486), E540 (= E489)
binding glutamic acid: C1 (= C2), R94 (= R74), W95 (= W75), T97 (= T77), H107 (= H87), G123 (= G100), D147 (= D124)
binding magnesium ion: S434 (≠ V383), R435 (= R384), T437 (≠ S386)
binding uridine-diphosphate-n-acetylgalactosamine: Q289 (≠ E234), R322 (= R271), G334 (≠ E281), G424 (≠ A373), T426 (≠ C375), S434 (≠ V383), T437 (≠ S386), C439 (≠ L388), G440 (≠ A389), V441 (≠ L390), H442 (≠ M391)

6r4eA Crystal structure of human gfat-1 in complex with glucose-6-phosphate and l-glu (see paper)
38% identity, 100% coverage: 2:609/609 of query aligns to 1:663/663 of 6r4eA

query
sites
6r4eA

C
|
C

G

G

I

I

V<