SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_007704698.1 NCBI__GCF_001277175.1:WP_007704698.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

P46318 Lichenan-specific phosphotransferase enzyme IIB component; EIIB-Lic; PTS system lichenan-specific EIIB component; EC 2.7.1.- from Bacillus subtilis (strain 168) (see paper)
44% identity, 92% coverage: 3:96/102 of query aligns to 2:95/102 of P46318

query
sites
P46318

N

N

I

I

V

L

L

L

C

V

C

C

A

A

G

G

M

M

S

S

T

T

S

S

M

L

L

L

V

V

Q

S

R

K

M

M

K

E

D

K

A

S

A

A

Q

Q

K

E

K

Q

G

G

V

K

E

D

V

Y

A

T

I

I

K

W

A

A

V

V

P
x
S

V

G

A

D

E

S

F

V

K

Q

D

N

I

H

I

I

G

D

T

K

A

A

D

D

I

V

V

L

L

L

G

G

P

P

Q

Q

V

V

K

R

Y

Y

E

M

Q

L

P

P

K

Q

F

L

Q

K

E

K

I

L

A

G

D

E

P

S

L

K

G

G

K

V

K

P

V

V

A

D

V

V

I

I

D

N

M

T

M

V

D

H

Y

Y

G

G

M

T

M

C

K

N

G

G

D

A

V

E

V

V

L

L

D

K

K

S

A

A

S37 (≠ P38) modified: Phosphoserine

2wy2D Nmr structure of the iiachitobiose-iibchitobiose phosphoryl transition state complex of the n,n'-diacetylchitoboise brance of the e. Coli phosphotransferase system. (see paper)
45% identity, 90% coverage: 2:93/102 of query aligns to 2:91/103 of 2wy2D

query
sites
2wy2D

K

K

N

H

I

I

V

Y

L

L

C

F

C
|
C

A
x
S

A
|
A

G
|
G

M
|
M

S
|
S

T

T

S

S

M

L

L

L

V

V

Q

S

R

K

M

M

K

R

D

A

A

Q

A

A

Q

E

K

K

K

Y

G

E

V

V

E

P

V

V

A

I

I

I

K

E

A

A

V

F

P

P

V

E

A

T

E

L

F

A

K

G

D

E

I

K

I

G

G

Q

T

N

A

A

D

D

I

V

V

V

L

L

G

G

P

P

Q

Q

V

I

K

A

Y

Y

E

M

Q

L

P

P

K

E

F

I

Q

Q

E

R

I

L

A

L

D

P

P

-

L

-

G

N

K

K

K

P

V

V

A

E

V

V

I

I

D

D

M

S

M

L

D

L

Y

Y

G

G

M

K

M

V

K

D

G

G

D

L

V

G

V

V

L

L

binding phosphite ion: C8 (= C8), S9 (≠ A9), A10 (= A10), G11 (= G11), M12 (= M12), S13 (= S13)

P69795 PTS system N,N'-diacetylchitobiose-specific EIIB component; EIIB-Chb; IVcel; N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIB component; EC 2.7.1.196 from Escherichia coli (strain K12) (see 2 papers)
45% identity, 90% coverage: 2:93/102 of query aligns to 4:93/106 of P69795

query
sites
P69795

K

K

N

H

I

I

V

Y

L

L

C

F

C
|
C

A

S

A

A

G

G

M

M

S

S

T

T

S

S

M

L

L

L

V

V

Q

S

R

K

M

M

K

R

D

A

A

Q

A

A

Q

E

K

K

K

Y

G

E

V

V

E

P

V

V

A

I

I

I

K

E

A

A

V

F

P

P

V

E

A

T

E

L

F

A

K

G

D

E

I

K

I

G

G

Q

T

N

A

A

D

D

I

V

V

V

L

L

G

G

P

P

Q

Q

V

I

K

A

Y

Y

E

M

Q

L

P

P

K

E

F

I

Q

Q

E

R

I

L

A

L

D

P

P

-

L

-

G

N

K

K

K

P

V

V

A

E

V

V

I

I

D

D

M

S

M

L

D

L

Y

Y

G

G

M

K

M

V

K

D

G

G

D

L

V

G

V

V

L

L

C10 (= C8) active site, Phosphocysteine intermediate; mutation to S: Unable to be phosphorylated by EIIA.

Query Sequence

>WP_007704698.1 NCBI__GCF_001277175.1:WP_007704698.1
MKNIVLCCAAGMSTSMLVQRMKDAAQKKGVEVAIKAVPVAEFKDIIGTADIVLLGPQVKY
EQPKFQEIADPLGKKVAVIDMMDYGMMKGDVVLDKALKMLEQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory