SitesBLAST

Q8NLB7 Gentisate transporter from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / BCRC 11384 / CCUG 27702 / LMG 3730 / NBRC 12168 / NCIMB 10025 / NRRL B-2784 / 534) (see paper)
27% identity, 46% coverage: 18:228/456 of query aligns to 39:230/444 of Q8NLB7

query
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Q8NLB7

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D54 (≠ E31) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
D57 (= D34) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
R103 (= R87) mutation to A: Loss of transport activity.

Sites not aligning to the query:

309 W→V: Loss of transport activity.
312 D→A: Loss of transport activity.
313 R→A: Loss of transport activity.
317 mutation I->H,Y: Loss of transport activity.
386 R→A: Loss of transport activity.

A0A0H2VG78 Glucose transporter GlcP; Glucose/H(+) symporter from Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200) (see paper)
24% identity, 68% coverage: 82:393/456 of query aligns to 63:395/446 of A0A0H2VG78

query
sites
A0A0H2VG78

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R102 (= R129) mutation to A: Loss of transport activity.
I105 (≠ Q132) mutation to S: Affects symport activity. May function as an uniporter.
E122 (= E149) mutation to A: Loss of transport activity.
Q137 (= Q164) mutation to A: Loss of transport activity.
Q250 (≠ A258) mutation to A: Loss of transport activity.
Q251 (≠ Y259) mutation to A: Loss of transport activity.
N256 (= N264) mutation to A: Loss of transport activity.
W357 (vs. gap) mutation to A: Loss of transport activity.

Sites not aligning to the query:

22 D→N: Affects symport activity. May function as an uniporter.

P38695 Probable glucose transporter HXT5 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
23% identity, 66% coverage: 76:377/456 of query aligns to 149:489/592 of P38695

query
sites
P38695

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Sites not aligning to the query:

61 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

5c65A Structure of the human glucose transporter glut3 / slc2a3
24% identity, 69% coverage: 62:377/456 of query aligns to 61:393/457 of 5c65A

query
sites
5c65A

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Q

R

G

V

P

R

R

Y

P

S

A

A

A

A

M

S

A

I

V

A

A

binding Octyl Glucose Neopentyl Glycol : F75 (≠ V79), S76 (≠ V80), L79 (≠ H83), R87 (≠ K91), L95 (= L99), L96 (= L100), L121 (= L130), P199 (vs. gap)
binding cholesterol hemisuccinate: I270 (≠ G279)

Sites not aligning to the query:

5eqiA Human glut1 in complex with cytochalasin b (see paper)
29% identity, 37% coverage: 62:228/456 of query aligns to 59:212/447 of 5eqiA

query
sites
5eqiA

L

L

S

S

F

V

A

A

T

I

F

F

A

S

V

V

G

G

F

G

L

M

A

-

R

-

P

-

L

I

G

G

G

S

V

F

V

S

M

V

G

G

H

L

F

F

G

V

D

N

R

R

I

F

G

G

R

R

K

R

K

N

V

-

L

-

V

-

L

-

A

S

L

M

L

L

L

M

M

M

G

N

A

L

T

A

F

F

L

V

V

S

G

A

V

V

L

L

P

M

T

G

S

F

A

S

Q

K

I

L

G

G

V

K

W

-

A

S

P

F

I

E

L

M

L

L

V

I

L

L

L

G

R

R

L

F

I

I

Q

I

G

G

F

V

G

Y

V

C

G

G

G

L

E

T

W
x
T

G

G

G

F

A

V

V

P

L

M

T

Y

A

V

V

G

E

E

Y

V

A

S

P

P

K

T

N

A

R

L

R

R

G

G

F

A

Y

L

G

G

S

T

M

L

P

H

Q
|
Q

V

L

G

G

V
x
I

P

V

A

V

G

G

L

I

L

L

L

I

A

A

T

Q

A

-

V

-

M

V

F

F

G

G

T

L

K

D

A

S

L

I

T

M

G

G

D

N

Q

K

F

D

L

L

V

-

W

W

G

-

W

-

R

-

I

-

P

P

F

L

L

L

S

S

I

I

A

I

L

F

V

I

A

P

V

A

G

L

L

L

Y

Q

-

C

-

I

I

V

R

L

T

P

A

F

L

C

E

P

E

E

T

S

P

P

A

R

F

F

A

L

R

L

V

I

K

N

Q

R

Q

N

N

E

binding Cytochalasin B: T129 (≠ W140), Q153 (= Q164), I156 (≠ V167)

Sites not aligning to the query:

binding Cytochalasin B: 274, 376, 380, 403, 404

5eqhA Human glut1 in complex with inhibitor (2~{s})-3-(2-bromophenyl)-2-[2- (4-methoxyphenyl)ethanoylamino]-~{n}-[(1~{s})-1- phenylethyl]propanamide (see paper)
29% identity, 37% coverage: 62:228/456 of query aligns to 59:212/447 of 5eqhA

query
sites
5eqhA

L

L

S

S

F

V

A

A

T

I

F

F

A

S

V

V

G

G

F

G

L

M

A

-

R

-

P

-

L

I

G

G

G
x
S

V

F

V

S

M

V

G

G

H

L

F

F

G

V

D

N

R

R

I

F

G

G

R

R

K

R

K

N

V

-

L

-

V

-

L

-

A

S

L

M

L

L

L

M

M

M

G

N

A

L

T

A

F

F

L

V

V

S

G

A

V

V

L

L

P

M

T

G

S

F

A

S

Q

K

I

L

G

G

V

K

W

-

A

S

P

F

I

E

L

M

L

L

V

I

L

L

L

G

R

R

L

F

I

I

Q

I

G

G

F

V

G

Y

V

C

G

G

G

L

E

T

W
x
T

G

G

G

F

A

V

V

P

L

M

T

Y

A

V

V

G

E

E

Y

V

A

S

P

P

K

T

N

A

R

L

R

R

G

G

F

A

Y

L

G

G

S

T

M

L

P

H

Q

Q

V

L

G

G

V

I

P

V

A

V

G

G

L

I

L

L

L

I

A

A

T

Q

A

-

V

-

M

V

F

F

G

G

T

L

K

D

A

S

L

I

T

M

G

G

D

N

Q

K

F

D

L

L

V

-

W

W

G

-

W

-

R

-

I

-

P

P

F

L

L

L

S

S

I

I

A

I

L

F

V

I

A

P

V

A

G

L

L

L

Y

Q

-

C

-

I

I

V

R

L

T

P

A

F

L

C

E

P

E

E

T

S

P

P

A

R

F

F

A

L

R

L

V

I

K

N

Q

R

Q

N

N

E

binding (2~{S})-3-(2-bromophenyl)-2-[2-(4-methoxyphenyl)ethanoylamino]-~{N}-[(1~{S})-1-phenylethyl]propanamide: S72 (≠ G78), T129 (≠ W140)

Sites not aligning to the query:

binding (2~{S})-3-(2-bromophenyl)-2-[2-(4-methoxyphenyl)ethanoylamino]-~{N}-[(1~{S})-1-phenylethyl]propanamide: 274, 371, 380, 400, 403, 404

5eqgA Human glut1 in complex with inhibitor (2~{s})-3-(4-fluorophenyl)-2-[2- (3-hydroxyphenyl)ethanoylamino]-~{n}-[(1~{s})-1- phenylethyl]propanamide (see paper)
29% identity, 37% coverage: 62:228/456 of query aligns to 59:212/447 of 5eqgA

query
sites
5eqgA

L

L

S

S

F

V

A

A

T

I

F

F

A

S

V

V

G

G

F

G

L

M

A

-

R

-

P

-

L

I

G

G

G

S

V

F

V

S

M

V

G

G

H

L

F

F

G

V

D

N

R

R

I

F

G

G

R

R

K

R

K

N

V

-

L

-

V

-

L

-

A

S

L

M

L

L

L

M

M

M

G

N

A

L

T

A

F

F

L

V

V

S

G

A

V

V

L

L

P

M

T

G

S

F

A

S

Q

K

I

L

G

G

V

K

W

-

A

S

P

F

I

E

L

M

L

L

V

I

L

L

L

G

R

R

L

F

I

I

Q

I

G

G

F

V

G

Y

V

C

G

G

G

L

E

T

W
x
T

G

G

G

F

A

V

V

P

L

M

T

Y

A

V

V

G

E

E

Y

V

A

S

P

P

K

T

N

A

R

L

R

R

G

G

F

A

Y

L

G

G

S

T

M

L

P

H

Q

Q

V

L

G

G

V

I

P

V

A

V

G

G

L

I

L

L

L

I

A

A

T

Q

A

-

V

-

M

V

F

F

G

G

T

L

K

D

A

S

L

I

T

M

G

G

D

N

Q

K

F

D

L

L

V

-

W

W

G

-

W

-

R

-

I

-

P

P

F

L

L

L

S

S

I

I

A

I

L

F

V

I

A

P

V

A

G

L

L

L

Y

Q

-

C

-

I

I

V

R

L

T

P

A

F

L

C

E

P

E

E

T

S

P

P

A

R

F

F

A

L

R

L

V

I

K

N

Q

R

Q

N

N

E

binding (2~{S})-3-(4-fluorophenyl)-2-[2-(3-hydroxyphenyl)ethanoylamino]-~{N}-[(1~{S})-1-phenylethyl]propanamide: T129 (≠ W140)

Sites not aligning to the query:

binding (2~{S})-3-(4-fluorophenyl)-2-[2-(3-hydroxyphenyl)ethanoylamino]-~{N}-[(1~{S})-1-phenylethyl]propanamide: 274, 380, 403, 404

Q5EXK5 3-hydroxybenzoate transporter MhbT from Klebsiella oxytoca (see paper)
29% identity, 32% coverage: 82:226/456 of query aligns to 78:208/452 of Q5EXK5

query
sites
Q5EXK5

G

G

H

P

F

L

G

S

D
|
D

R

R

I

F

G

G

R

R

K

K

K

R

V

V

L

I

V

E

L

F

A

C

L

V

L

A

L

L

M

F

G

G

A

L

A

F

T

S

F

L

L

L

V

S

G

A

V

F

L

A

P

P

T

D

S

L

A

Q

Q

-

I

-

G

-

V

-

W

-

A

-

P

-

I

-

L

M

L

L

V

V

L

F

L

L

R

R

L

F

I

L

Q

T

G

G

F

L

G

G

V

L

G

G

E

A

W

M

G

P

G

N

A

T

V

I

L

T

T

M

A

T

V

S

E

E

Y

Y

A

L

P

P

K

A

N

R

R

R

G

G

F

A

Y

L

G

V

S

T

M

L

P

M

Q

F

V

C

G

G

V

F

P

T

A

L

G

G

L

S

L

A

L

F

A

G

T

G

A

V

V

V

M

S

F

A

G

Q

T

L

K

V

A

P

L

L

T

I

G

G

-

W

D

H

Q

G

F

I

L

L

V

V

W

L

G

G

W

G

R

V

I

L

P

P

F

L

L

I

L

L

S

A

I

V

A

A

L

L

V

V

A

P

V

L

G

-

L

-

Y

-

I

-

R

-

T

-

A

-

L

L

E

P

E

E

T

S

P

P

A

R

F

W

A

Q

R

I

V

R

K

R

Q

Q

D82 (= D86) mutation to A: Loss of activity.

Sites not aligning to the query:

311 V→W: Loss of activity.
314 D→A: Loss of activity.

P11169 Solute carrier family 2, facilitated glucose transporter member 3; Glucose transporter type 3, brain; GLUT-3 from Homo sapiens (Human) (see paper)
23% identity, 69% coverage: 62:377/456 of query aligns to 65:405/496 of P11169

query
sites
P11169

L

L

S

S

F

V

A

A

T

I

F

F

A

S

V

V

G

G

F

G

L

M

A

-

R

-

P

-

L

I

G

G

G

S

V

F

V

S

M

V

G

G

H

L

F

F

G

V

D

N

R

R

I

F

G

G

R

R

K

R

K

N

V

S

L

M

V

L

L

I

A

V

L

N

L

L

M

A

G

V

A

T

A

G

T

G

F

C

L

F

V

M

G

G

V

L

L

C

P

K

T

V

S

A

A

K

Q

S

I

V

G

E

V

-

W

-

A

-

P

-

I

-

L

M

L

L

V

I

L

L

L

G

R

R

L

L

I

V

Q

I

G

G

F

L

G

F

V

C

G

G

G

L

E

C

W

T

G

G

G

F

A

V

V

P

L

M

T

Y

A

I

V

G

E

E

Y

I

A

S

P

P

K

T

N

A

R

L

R

R

G

G

F

A

Y

F

G

G

S

T

M

L

P

N

Q
|
Q

V

L

G

G

V

I

P

V

A

V

G

G

L

I

L

L

L

V

A

A

T

Q

A

-

V

-

M

I

F

F

G

G

T

L

K

E

A

F

L

I

T

L

G

G

D

S

Q

E

F

E

L

L

V

-

W

W

G

-

W

-

R

-

I

-

P

P

F

L

L

L

S

G

I

F

A

T

L

I

V

L

A

P

V

A

G

-

L

-

Y

I

I

L

R

Q

T

S

A

A

-

A

-

L

-

P

-

F

L

C

E

P

E

E

T

S

P

P

A

R

F

F

-

L

-

I

A

N

R

R

V

K

K

E

Q

E

N

N

T

A

A

K

V

Q

K

I

I

L

P

Q

M

R

V

L

D

W

A

G

F

T

K

Q

N

D

Y

V

P

S

R

Q

Q

D

I

I

-

Q

-

E

I

M

L

K

A

D

A

E

G

S

S

A

M

R

I

M

S

S

T

Q

G

E

A

K

Y

Q

F

V

Y

T

I

V

L

L

N

E

T

L

Y

F

S

R

L

V

T

S

Y

S

A

Y

D

R

Q

Q

F

P

D

I

L

I

S

S

G

I

N

V

L

L

M
x
Q

L
|
L

G
x
S

-
x
Q

-

L

-

S

-

G

-

I

-
x
N

-

A

-

V

-

F

-

Y

-

S

-

T

-

G

-

I

-

F

-

K

-

D

-

A

-

G

-

V

-

Q

-

E

-

P

-

I

-

Y

A

A

V

T

M

I

V

G

S

A

A

G

L

V

V

V

A
x
N

V

T

I

I

F

F

I

T

P

V

I

V

F

S

G

L

Q

F

L

L

S

V

E

E

K

R

F

A

G

G

R

R

R

T

I

L

L

H

M

M

V

I

G

G

I

L

T

G

G

G

M

M

-

A

-

F

-

C

G

S

V

T

W

L

V

M

F

T

V

V

A

S

F

L

A

L

I

K

D

D

T

N

G

Y

N

N

-

G

-

M

F

S

W

F

I

V

T

C

T

I

G

G

A

A

L

I

A

L

V

V

G

F

A

V

F

A

M

F

L

F

S
x
E

I

I

S

G

Y

P

G

G

P

P

Q

I

A

P

T
x
W

F

F

I

I

-

V

A

A

E

E

L

L

F

F

D

S

A

Q

R

G

V

P

R

R

Y

P

S

A

A

A

A

M

S

A

I

V

A

A

Q159 (= Q164) binding D-glucose
QLS 277:279 (≠ MLG 282:284) Important for selectivity against fructose; mutation to HVA: Confers moderate fructose transport activity.
QQ 280:281 (vs. gap) binding D-glucose
N286 (vs. gap) binding D-glucose
N315 (≠ A293) binding D-glucose
E378 (≠ S351) binding D-glucose
W386 (≠ T359) binding D-glucose

P11166 Solute carrier family 2, facilitated glucose transporter member 1; Glucose transporter type 1, erythrocyte/brain; GLUT-1; HepG2 glucose transporter from Homo sapiens (Human) (see 23 papers)
29% identity, 38% coverage: 62:233/456 of query aligns to 67:227/492 of P11166

query
sites
P11166

L

L

S

S

F

V

A

A

T

I

F

F

A

S

V

V

G

G

F

G

L
x
M

A

-

R

-

P

-

L

I

G

G

G

S

V

F

V

S

M

V

G

G

H

L

F

F

G

V

D

N

R

R

I

F

G
|
G

R

R

K

R

K

N

V

-

L

-

V

-

L

-

A

S

L

M

L

L

L

M

M

M

G

N

A

L

T

A

F

F

L

V

V

S

G

A

V

V

L

L

P

M

T

G

S

F

A

S

Q

K

I

L

G

G

V

K

W

-

A

S

P

F

I

E

L

M

L

L

V

I

L

L

L

G

R
|
R

L

F

I

I

Q

I

G
|
G

F

V

G

Y

V

C

G

G

G

L

E

T

W
x
T

G

G

G

F

A

V

V

P

L

M

T

Y

A

V

V

G

E

E

Y

V

A

S

P
|
P

K

T

N

A

R

L

R
|
R

G

G

F

A

Y

L

G

G

S

T

M

L

P

H

Q

Q

V

L

G

G

V

I

P

V

A

V

G

G

L

I

L
|
L

L

I

A

A

T

Q

A

-

V

-

M

V

F

F

G

G

T

L

K

D

A

S

L

I

T

M

G

G

D

N

Q

K

F

D

L

L

V

-

W

W

G

-

W

-

R

-

I

-

P

P

F

L

L

L

S

S

I
|
I

A

I

L

F

V

I

A

P

V

A

G

L

L

L

Y

Q

-

C

-

I

I

V

R
x
L

T
x
P

A

F

L

C

E

P

E

E

T

S

P

P

A
x
R

F

F

A

L

R

L

V

I

-

N

-
x
R

K

N

Q

E

N

N

T
x
R

A

A

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K

K
x
S

I

V

M77 (≠ L72) to T: in EIG12; decreased glucose transport; dbSNP:rs1187210267
G91 (= G89) to D: in GLUT1DS1; significantly decreases the transport of 3-O-methyl-D-glucose; dbSNP:rs80359814
R126 (= R129) to C: in GLUT1DS1, GLUT1DS2 and DYT9; reduced transporter activity; dbSNP:rs80359818; to H: in GLUT1DS1; significantly decreases the transport of 3-O-methyl-D-glucose and dehydroascorbic acid; 57% of wild-type glucose uptake activity; dbSNP:rs80359816
G130 (= G133) to S: in GLUT1DS1; 75% of wild-type glucose uptake activity; dbSNP:rs80359819
T137 (≠ W140) binding cytochalasin B
P149 (= P152) to A: in EIG12; uncertain significance
R153 (= R156) to C: in GLUT1DS1; 44% of wild-type glucose uptake activity; dbSNP:rs1643479461
L169 (= L172) natural variant: Missing (in GLUT1DS1; 48% of wild-type glucose uptake activity; dbSNP:rs80359832)
I192 (= I202) mutation to C: Strongly decreases glucose transport.
L204 (≠ R212) mutation to C: Abolishes glucose transport.
P205 (≠ T213) mutation to C: Abolishes glucose transport.
R212 (≠ A220) to C: in GLUT1DS1 and DYT9; dbSNP:rs387907312
R218 (vs. gap) to S: in EIG12; decreased glucose transport
R223 (≠ T229) to P: in EIG12; mild phenotype; reduced transporter activity; impaired phosphorylation by PKC; dbSNP:rs397514564; to Q: in EIG12; uncertain significance; no effect on glucose transport; impaired phosphorylation by PKC; dbSNP:rs397514564; to W: in GLUT1DS1; impaired phosphorylation by PKC; dbSNP:rs796053248
S226 (≠ K232) modified: Phosphoserine; by PKC/PRKCB; mutation to A: Abolishes phosphorylation by PKA, leading to impaired response to TPA.

Sites not aligning to the query:

34 N → S: in GLUT1DS1; 55% of wild-type glucose uptake activity; dbSNP:rs80359812
45 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→T: Loss of glycosylation site.
51 R → H: in EIG12; uncertain significance; dbSNP:rs201815571
60 T → M: in EIG12; uncertain significance; decreased glucose transport; dbSNP:rs142986731
232 R → C: in EIG12; the mutant protein is expressed at the cell surface but has mildly decreased glucose uptake (70%) compared to wild-type; dbSNP:rs387907313
243 E → V: in EIG12; decreased glucose transport; dbSNP:rs2124449030
275 A → T: in GLUT1DS2; the mutation decreases glucose transport but does not affect cation permeability; dbSNP:rs121909740
282 binding cytochalasin B
282:285 natural variant: Missing (in GLUT1DS2; accompanied by hemolytic anemia and altered erythrocyte ion concentrations; the mutation decreases glucose transport and causes a cation leak that alteres intracellular concentrations of sodium potassium and calcium)
286 G → D: in SDCHCN; no effect on protein abundance; no effect on localization to the plasma membrane; loss of D-glucose transporter activity; increased cation leakage; dbSNP:rs864309514
295 T → M: in GLUT1DS1; 75% of wild-type glucose uptake activity; dbSNP:rs80359823
303 V → L: found in a patient with GLUT1 deficiency syndrome; dbSNP:rs1205631854
314 G → S: in GLUT1DS2; the mutation decreases glucose transport but does not affect cation permeability; dbSNP:rs121909739
324 S → L: in GLUT1DS2; mild phenotype; reduced transporter activity; dbSNP:rs796053253
329 E → Q: in GLUT1DS1; stabilizes the inward-open conformation
333 R → Q: in GLUT1DS1 and GLUT1DS2; dbSNP:rs1553155986; R → W: in GLUT1DS1; 43% of wild-type glucose uptake activity; dbSNP:rs80359825
340 G→C: Strongly decreases glucose transport.
388 binding cytochalasin B
411 Not glycosylated; binding cytochalasin B; N → S: in EIG12; decreased glucose transport; dbSNP:rs398123069
435 natural variant: Missing (in SDCHCN; no effect on protein abundance; no effect on localization to the plasma membrane; loss of D-glucose transporter activity; increased cation leakage)
458 R → W: in EIG12; decreased glucose transport; dbSNP:rs13306758
485 P → L: in GLUT1DS1; creates a dileucine internalization motif that promotes recruitment of clathrin and mislocalization of the protein to endocytic compartments; dbSNP:rs1159593580

P10870 Low glucose sensor SNF3; High-affinity glucose receptor SNF3; High-affinity transporter-like sensor SNF3; Sucrose nonfermenting protein 3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 2 papers)
28% identity, 37% coverage: 58:227/456 of query aligns to 146:296/884 of P10870

query
sites
P10870

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G153 (≠ A65) mutation to R: In SNF3-72.
R229 (= R156) mutation to K: In SNF3-1; constitutively signaling glucose receptor.

Sites not aligning to the query:

112 G→D: In SNF3-142.
374 I→V: Decreases responsiveness to mannose and fructose.
402 V→I: In SNF3-39.
462 F→Y: Decreases responsiveness to fructose.

P32467 Low-affinity glucose transporter HXT4; Low-affinity glucose transporter LGT1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
25% identity, 57% coverage: 76:337/456 of query aligns to 134:419/576 of P32467

query
sites
P32467

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Sites not aligning to the query:

45 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

7spsA Crystal structure of human glucose transporter glut3 bound with exofacial inhibitor sa47 (see paper)
23% identity, 69% coverage: 62:377/456 of query aligns to 62:402/468 of 7spsA

query
sites
7spsA

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binding methyl N-[(2-{4-[4-(5-fluoro-2-methoxyphenyl)piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidin-1-yl}phenyl)methyl]-beta-alaninate: Q156 (= Q164), I163 (≠ L171), Q278 (vs. gap), F286 (vs. gap), A308 (≠ S289), N312 (≠ A293), F374 (≠ L350), E375 (≠ S351)

Sites not aligning to the query:

binding methyl N-[(2-{4-[4-(5-fluoro-2-methoxyphenyl)piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidin-1-yl}phenyl)methyl]-beta-alaninate: 21, 25, 29, 406, 407, 410

7crzA Crystal structure of human glucose transporter glut3 bound with c3361 (see paper)
23% identity, 69% coverage: 62:377/456 of query aligns to 63:403/469 of 7crzA

query
sites
7crzA

L

L

S

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A

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S

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S

S

A

M

R

I

M

S

S

T

Q

G

E

A

K

Y

Q

F

V

Y

T

I

V

L

L

N

E

T

L

Y

F

S

R

L

V

T

S

Y

S

A

Y

D

R

Q

Q

F

P

D

I

L

I

S

S

G

I

N

V

L

L

M

Q

L

L

G

S

-
x
Q

-

L

-

S

-

G

-

I

-
x
N

-

A

-

V

-

F

-

Y

-

S

-

T

-

G

-

I

-

F

-

K

-

D

-

A

-

G

-

V

-

Q

-

E

-

P

-

I

-

Y

A

A

V

T

M

I

V

G

S

A

A

G

L

V

V

V

A
x
N

V

T

I

I

F

F

I

T

P

V

I

V

F

S

G

L

Q

F

L

L

S

V

E

E

K

R

F

A

G

G

R

R

R

T

I

L

L

H

M

M

V

I

G

G

I

L

T

G

G

G

M

M

-

A

-

F

-

C

G

S

V

T

W

L

V

M

F

T

V

V

A

S

F

L

A

L

I

K

D

D

T

N

G

Y

N

N

-

G

-

M

F

S

W

F

I

V

T

C

T

I

G

G

A

A

L

I

A

L

V

V

G

F

A

V

F

A

M

F

L
x
F

S

E

I

I

S

G

Y

P

G

G

P

P

Q

I

A

P

T
x
W

F

F

I

I

-

V

A

A

E

E

L

L

F

F

D

S

A

Q

R

G

V

P

R

R

Y

P

S

A

A

A

A

M

S

A

I

V

A

A

binding (2S,3R,4S,5R,6R)-6-(hydroxymethyl)-4-undec-10-enoxy-oxane-2,3,5-triol: A66 (= A65), S69 (≠ A68), Q157 (= Q164), I164 (≠ L171), Q278 (vs. gap), Q279 (vs. gap), N284 (vs. gap), N313 (≠ A293), F375 (≠ L350), W384 (≠ T359)

Sites not aligning to the query:

binding (2S,3R,4S,5R,6R)-6-(hydroxymethyl)-4-undec-10-enoxy-oxane-2,3,5-triol: 26, 411, 412, 415

O15244 Solute carrier family 22 member 2; Organic cation transporter 2; hOCT2 from Homo sapiens (Human) (see 8 papers)
28% identity, 29% coverage: 65:197/456 of query aligns to 154:284/555 of O15244

query
sites
O15244

A

S

T

S

F

V

A

N

V

V

G

G

F

F

L

F

A

-

R

-

P

-

L

I

G

G

G

S

V
x
M

V

S

M

I

G

G

H
x
Y

F

I

G

A

D

D

R

R

I

F

G

G

R

R

K

K

K

L

V

C

L

L

V

L

L

T

A

T

L

V

L

L

L

I

M

N

G

A

A

A

T

G

F

V

L

L

V

M

G

A

V

I

L

S

P

P

T

T

S

Y

A
x
T

Q

-

I

-

G

-

V

-

W

W

A

-

P

-

I

-

L

-

L

M

V

L

L

I

L

F

R

R

L

L

I

I

Q

Q

G

G

F

L

G

V

V

S

G

K

G

A

E

G

W

W

G

L

G

I

A

G

V

Y

L

I

T

L

A

I

V

T

E

E

Y

F

A

V

P

G

K

R

N

R

R

Y

R

R

G

R

F

T

Y

V

G

G

S

I

M

F

P
x
Y

Q

Q

V

V

G

A

V

Y

P

T

A

V

G

G

L

L

L

V

A

L

T

A

A

G

V

V

M

A

F
x
Y

G

A

-

L

-

P

-

H

-

W

-

R

-

W

-

L

-

Q

-

F

T

T

K

V

A
x
S

L

L

T

P

G

N

D

F

Q

F

F

F

L

L

V

L

W
x
Y

G
x
Y

W

W

R

C

I

I

P
|
P

M165 (≠ V79) to I: lower Vmax for MPP(+) transport; no change in transport efficiency (Vmax/Km) and clearance of cyclo(his-pro) and salsolinol; dbSNP:rs8177507
Y169 (≠ H83) mutation to F: No change in TEA uptake.
T201 (≠ A115) to M: in dbSNP:rs145450955
Y241 (≠ P163) mutation to F: Slight decrease in TEA uptake. No change in tyrosine phosphorylation. Strong decrease in TEA uptake; when associated with F-362. Strong decrease in TEA and metformin uptake and YES1-mediated tyrosine phosphorylation; when associated with F-362 and F-377.
Y257 (≠ F179) mutation to F: No change in TEA uptake.
S270 (≠ A183) to A: decreased Ki value for TBA inhibition of MPP(+); no change in transport efficiency (Vmax/Km) and clearance of cyclo(his-pro) and salsolinol; dbSNP:rs316019
Y279 (≠ W192) mutation to F: No change in TEA uptake.
Y280 (≠ G193) mutation to F: No change in TEA uptake.
P284 (= P197) mutation to A: Decreased TEA and metformin uptake. Decreased tyrosine phosphorylation.

Sites not aligning to the query:

54 P → S: in dbSNP:rs8177504
73 Y→F: No change in TEA uptake.
92 Y→F: No change in TEA uptake.
128 Y→F: No change in TEA uptake.
284:288 Proline-rich sequence
286 S→A: No change in TEA and metformin uptake. No change in tyrosine phosphorylation.
287 P→A: Decreased TEA and metformin uptake. Decreased tyrosine phosphorylation.
362 Y→F: Decreased TEA uptake and YES1-mediated tyrosine phosphorylation. Strong decrease in TEA uptake; when associated with F-241. Strong decrease in TEA uptake; when associated with F-377. Strong decrease in TEA and metformin uptake and YES1-mediated tyrosine phosphorylation; when associated with F-241 and F-377.
377 Y→F: Slight decrease in TEA uptake. No change in tyrosine phosphorylation. Strong decrease in TEA uptake; when associated with F-362. Strong decrease in TEA and metformin uptake and YES1-mediated tyrosine phosphorylation; when associated with F-241 and F-362.
400 R → C: lower Vmax and reduced Ki value for TBA inhibition of MPP(+); lower transport efficiency (Vmax/Km) and clearance of cyclo(his-pro); no change in transport efficiency (Vmax/Km) and clearance of salsolinol; dbSNP:rs8177516
432 K → Q: lower Km value for MPP(+) and reduced Ki value for TBA inhibition of MPP; no change in transport efficiency (Vmax/Km) and clearance of cyclo(his-pro) and salsolinol; dbSNP:rs8177517
458 Y→F: No change in TEA uptake.
544 Y→F: No change in TEA uptake.

4zw9A Crystal structure of human glut3 bound to d-glucose in the outward- occluded conformation at 1.5 angstrom (see paper)
24% identity, 69% coverage: 62:377/456 of query aligns to 65:405/470 of 4zw9A

query
sites
4zw9A

L

L

S

S

F

V

A

A

T

I

F

F

A

S

V

V

G

G

F

G

L

M

A

-

R

-

P

-

L

I

G

G

G

S

V

F

V

S

M

V

G

G

H

L

F

F

G

V

D

N

R

R

I

F

G

G

R

R

K

R

K

N

V

S

L

M

V

L

L

I

A

V

L

N

L

L

M

A

G

V

A

T

A

G

T

G

F

C

L

F

V

M

G

G

V

L

L

C

P

K

T

V

S

A

A

K

Q

S

I

V

G

E

V

-

W

-

A

-

P

-

I

-

L

M

L

L

V

I

L

L

L

G

R

R

L

L

I

V

Q

I

G

G

F

L

G

F

V

C

G

G

G

L

E

C

W

T

G

G

G

F

A

V

V

P

L

M

T

Y

A

I

V

G

E

E

Y

I

A

S

P

P

K

T

N

A

R

L

R

R

G

G

F

A

Y

F

G

G

S

T

M

L

P

N

Q
|
Q

V

L

G

G

V
x
I

P

V

A

V

G

G

L
x
I

L

L

L

V

A

A

T

Q

A

-

V

-

M

I

F

F

G

G

T

L

K

E

A

F

L

I

T

L

G

G

D

S

Q

E

F

E

L

L

V

-

W

W

G

-

W

-

R

-

I

-

P

P

F

L

L

L

S

G

I

F

A

T

L

I

V

L

A

P

V

A

G

I

L

L

Y

Q

I

-

R

S

T

A

A

A

L

L

-

P

-

F

-

C

E

P

E

E

T

S

P

P

A

R

F

F

-

L

-

I

A

N

R

R

V

K

K

E

Q

E

N

N

T

A

A

K

V

Q

K

I

I

L

P

Q

M

R

V

L

D

W

A

G

F

T

K

Q

N

D

Y

V

P

S

R

Q

Q

D

I

I

-

Q

-

E

I

M

L

K

A

D

A

E

G

S

S

A

M

R

I

M

S

S

T

Q

G

E

A

K

Y

Q

F

V

Y

T

I

V

L

L

N

E

T

L

Y

F

S

R

L

V

T

S

Y

S

A

Y

D

R

Q

Q

F

P

D

I

L

I

S

S

G

I

N

V

L

L

M

Q

L

L

G

S

-
x
Q

-

L

-

S

-

G

-

I

-
x
N

-

A

-

V

-

F

-

Y

-

S

-

T

-

G

-

I

-

F

-

K

-

D

-

A

-

G

-

V

-

Q

-

E

-

P

-

I

-

Y

A

A

V

T

M

I

V

G

S

A

A

G

L

V

V

V

A

N

V

T

I

I

F

F

I

T

P

V

I

V

F

S

G

L

Q

F

L

L

S

V

E

E

K

R

F

A

G

G

R

R

R

T

I

L

L

H

M

M

V

I

G

G

I

L

T

G

G

G

M

M

-

A

-

F

-

C

G

S

V

T

W

L

V

M

F

T

V

V

A

S

F

L

A

L

I

K

D

D

T

N

G

Y

N

N

-

G

-

M

F

S

W

F

I

V

T

C

T

I

G

G

A

A

L

I

A

L

V

V

G

F

A

V

F

A

M

F

L
x
F

S

E

I

I

S

G

Y

P

G

G

P

P

Q

I

A

P

T
x
W

F

F

I

I

-

V

A

A

E

E

L

L

F

F

D

S

A

Q

R

G

V

P

R

R

Y

P

S

A

A

A

A

M

S

A

I

V

A

A

binding beta-D-glucopyranose: Q159 (= Q164), I166 (≠ L171), Q280 (vs. gap), Q281 (vs. gap), N286 (vs. gap), F377 (≠ L350), W386 (≠ T359)
binding alpha-D-glucopyranose: Q159 (= Q164), I162 (≠ V167), I166 (≠ L171), Q280 (vs. gap), Q281 (vs. gap), N286 (vs. gap), W386 (≠ T359)

7sptA Crystal structure of exofacial state human glucose transporter glut3 (see paper)
23% identity, 69% coverage: 62:377/456 of query aligns to 65:405/470 of 7sptA

query
sites
7sptA

L

L

S

S

F

V

A

A

T

I

F

F

A

S

V

V

G

G

F

G

L

M

A

-

R

-

P

-

L

I

G

G

G

S

V

F

V

S

M

V

G

G

H

L

F

F

G

V

D

N

R

R

I

F

G

G

R

R

K

R

K

N

V

S

L

M

V

L

L

I

A

V

L

N

L

L

M

A

G

V

A

T

A

G

T

G

F

C

L

F

V

M

G

G

V

L

L

C

P

K

T

V

S

A

A

K

Q

S

I

V

G

E

V

-

W

-

A

-

P

-

I

-

L

M

L

L

V

I

L

L

L

G

R

R

L

L

I

V

Q

I

G

G

F

L

G

F

V

C

G

G

G

L

E

C

W

T

G

G

G

F

A

V

V

P

L

M

T

Y

A

I

V

G

E

E

Y

I

A

S

P

P

K

T

N

A

R

L

R

R

G

G

F

A

Y

F

G

G

S

T

M

L

P

N