SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_008198284.1 NCBI__GCF_000166275.1:WP_008198284.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q89WV5 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) (see paper)
57% identity, 98% coverage: 11:630/630 of query aligns to 23:643/648 of Q89WV5

query
sites
Q89WV5

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G263 (= G249) mutation to I: Loss of activity.
G266 (= G252) mutation to I: Great decrease in activity.
K269 (= K255) mutation to G: Great decrease in activity.
E414 (= E400) mutation to Q: Great decrease in activity.

P27550 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Escherichia coli (strain K12) (see paper)
56% identity, 98% coverage: 11:630/630 of query aligns to 24:646/652 of P27550

query
sites
P27550

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K609 (= K593) modified: N6-acetyllysine; by autocatalysis

Q8ZKF6 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
55% identity, 98% coverage: 11:630/630 of query aligns to 24:646/652 of Q8ZKF6

query
sites
Q8ZKF6

Y

Y

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K

T

L

L

N

N

I

L

T

A

E

A

N

N

I

C

F

L

E

D

R

R

Y

H

L

L

F

Q

T

E

I

N

G

G

D

D

R

R

P

T

A

A

I

I

W

W

E

E

P

G

N

D

D

D

P

T

N

S

E

Q

A

S

G

-

R

K

T

H

L

I

T

S

Y

Y

R

R

E

E

L

L

F

H

E

R

E

D

V

V

S

C

R

R

F

F

S

A

N

N

A

T

L

L

K

L

S

D

K

L

G

G

I

I

G

K

K

K

G

G

D

D

K

V

V

V

I

A

I

I

Y

Y

M

M

P

P

M

M

V

V

P

P

E

E

A

A

V

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

I

V

H

H

S

S

V

V

V

I

F

F

A

G

G

G

F

F

S

S

S

P

N

E

A

A

L

V

A

A

D

G

R

R

I

I

N

I

D

D

C

S

E

S

A

S

K

R

A

L

V

V

L

I

T

T

S

A

D

D

G

E

N

G

F

V

R

R

G

A

S

G

K
x
R

K

S

I

I

A

P

V

L

K

K

A

K

V

N

V

V

D

D

E

D

A

A

L

L

T

K

K

N

-

P

-

N

-

V

S

T

S

S

V

V

E

E

T

H

V

V

I

I

V

V

Y

L

Q

K

R

R

T

T

H

G

Q

S

D

D

V

I

T

D

M

W

Q

Q

D

E

G

G

R

R

D

D

Y

L

W

W

H

R

D

D

V

L

V

I

A

E

D

K

E

A

S

S

K

P

D

E

C

H

P

Q

A

P

E

E

E

A

M

M

D

N

S

A

E

E

D

D

M

P

L

L

F

F

I

I

L

L

Y

Y

T

T

S

S

G

G

S

S

T

T

G

G

K

K

P

P

K

K

G

G

V

V

V

L

H

H

T

T

G

G

Y

Y

M

L

V

V

Y

Y

S

A

K

A

Y

T

S

T

F

F

E

K

N

Y

V

V

F

F

Q

D

Y

Y

S

H

P

P

G

G

D

D

V

I

Y

Y

W

W

C

C

T

T

A

A

D

D

V

V

G

G

W

W

I

V

T
|
T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

L

A

A

C

G

G

A

A

T

T

I

L

M

M

F

F

E

E

G

G

V

V

P

P

T
x
N

F

W

P

P

D

T

C

P

G

A

R

R

F

M

W

C

A

Q

I

V

V

V

E

D

K

K

Y

H

K

Q

V

V

N

N

Q

I

F

L

Y

Y

T

T

A
|
A

P

P

T

T

A

A

I

I

R

R

A

A

L

L

Q

M

A

A

Y

E

G

G

T

D

V

K

E

A

I

I

E

E

K

G

Y

T

D

D

L

R

S

S

L

L

K

R

V

I

L

L

G

G

S

S

V

V

G

G

E

E

P

P

I

I

N

N

E

P

E

E

A

A

W

W

H

E

W

W

Y

Y

H

W

T

K

H

K

I

I

G

G

K

K

N

E

K

K

C

C

P

P

I

V

V

V

D

D

T

T

W

W

Q

Q

T

T

E

E

T

T

G

G

I

F

M

M

V

I

S

T

P

P

I

L

A

P

G

G

I

A

T

I

P

E

T

L

K

K

P

A

A

G

Y

S

A

A

T

T

L

R

P

P

L

F

P

F

G

G

V

V

Q

Q

L

P

C

A

I

L

V

V

D

D

P

N

E

E

G

G

N

H

E

P

L

Q

T

E

G

G

N

-

S

A

V

T

E

E

G

G

N

N

L

L

C

V

I

I

K

T

F

D

P

S

W

W

P

P

S

G

M

Q

I

A

R

R

T

T

T

L

Y

F

G

G

D

D

H

H

D

E

R

R

C

F

K

E

Q

Q

T

T

Y

Y

F

F

S

S

T

T

Y

F

K

K

G

N

M

M

Y

Y

F

F

T

S

G

G

D

D

G

G

V

A

K

R

R

R

D

D

H

E

D

D

G

G

Y

Y

R

W

I

I

L

T

G

G

R

R

V

V

D
|
D

D

D

V

V

I

L

N

N

V

V

S
|
S

G
|
G

H

H

R
|
R

M

L

G

G

T

T

A

A

E

E

V

I

E

E

N

S

A

A

I

L

N

V

E

A

H

H

P

P

K

K

V

I

I

A

E

E

S

A

A

A

V

V

G

G

Y

I

P

P

H

H

D

A

V

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

A

A

Y

Y

V

V

I

T

C

L

D

N

L

H

T

G

N

E

R

E

T

P

E

S

D

P

N

E

L

L

V

Y

N

A

E

E

I

V

K

R

E

N

M

W

V

V

S
x
R

K

K

I

E

I

I

G

G

P

P

I

L

A

A

K

T

P

P

D

D

K

V

I

L

Q

H

L

W

V

T

P

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

V

I

A

A

E

A

N

G

N

D

L

T

D

S

N

N

M

L

G

G

D

D

T

T

S

S

T

T

L

L

L

A

D

D

P

P

D

G

V

V

E

E

K

K

I

L

D

E

G

E

R

K

K

Q

R194 (≠ K180) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 3-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 2-fold reduction for CoA.
T311 (= T294) binding CoA
N335 (≠ T318) binding CoA
A357 (= A340) mutation to V: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 3-fold reduction for CoA.
D517 (= D501) mutation to G: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 10-fold reduction for CoA.; mutation to P: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold reduction in the affinity for ATP and 4.5-fold reduction for CoA.
S523 (= S507) binding CoA
G524 (= G508) mutation to L: No acetyl-coenzyme A synthetase activity.; mutation to S: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. Almost the same affinity as the wild-type for ATP, but 9-fold reduction in the affinity for CoA.
R526 (= R510) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 4-fold reduction for CoA.
R584 (≠ S568) binding CoA; mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 7-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 8-fold reduction for CoA.
K609 (= K593) modified: N6-acetyllysine; by Pat; mutation to A: No acetyl-coenzyme A synthetase activity.

5jrhA Crystal structure of salmonella enterica acetyl-coa synthetase (acs) in complex with camp and coenzyme a (see paper)
55% identity, 98% coverage: 11:630/630 of query aligns to 20:639/640 of 5jrhA

query
sites
5jrhA

Y

Y

L

E

H

T

E

K

Y

Y

Q

K

K

Q

S

S

V

I

A

N

Q

D

P

P

E

D

E

T

F

F

W

W

A

G

R

E

I

Q

A

G

D

K

S

I

F

L

H

D

W

W

R

I

K

T

R

P

W

Y

D

Q

K

K

T

V

L

K

K

N

W

T

N

S

F

F

E

A

G

P

P

G

D

N

V

V

-

S

-

I

K

K

W

W

F

Y

L

E

N

D

G

G

K

T

L

L

N

N

I

L

T

A

E

A

N

N

I

C

F

L

E

D

R

R

Y

H

L

L

F

Q

T

E

I

N

G

G

D

D

R

R

P

T

A

A

I

I

W
|
W

E

E

P

G

N

D

D

D

P

T

N

S

E

Q

A

S

G

-

R

K

T

H

L

I

T

S

Y

Y

R

R

E

E

L

L

F

H

E

R

E

D

V

V

S

C

R

R

F

F

S

A

N

N

A

T

L

L

K

L

S

D

K

L

G

G

I

I

G

K

K

K

G

G

D

D

K

V

V

V

I

A

I

I

Y

Y

M

M

P

P

M

M

V

V

P

P

E
|
E

A

A

V

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

I

V

H

H

S

S

V

V

V

I

F
|
F

A
x
G

G
|
G

F

F

S

S

S

P

N

E

A

A

L

V

A

A

D
x
G

R

R

I

I

N

I

D

D

C

S

E

S

A

S

K

R

A

L

V

V

L

I

T

T

S

A

D

D

G

E

N

G

F

V

R
|
R

G

A

S

G

K

R

K

S

I

I

A

P

V

L

K

K

A

K

V

N

V

V

D

D

E

D

A

A

L

L

T

K

K

N

-

P

-

N

-

V

S

T

S

S

V

V

E

E

T

H

V

V

I

I

V

V

Y

L

Q

K

R

R

T

T

H

G

Q

S

D

D

V

I

T

D

M

W

Q

Q

D

E

G

G

R

R

D

D

Y

L

W

W

H

R

D

D

V

L

V

I

A

E

D

K

E

A

S

S

K

P

D

E

C

H

P

Q

A

P

E

E

E

A

M

M

D

N

S

A

E

E

D

D

M

P

L

L

F

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

K
|
K

P
|
P

K

K

G

G

V

V

V

L

H

H

T

T

G

G

Y

Y

M

L

V

V

Y

Y

S

A

K

A

Y

T

S

T

F

F

E

K

N

Y

V

V

F

F

Q

D

Y

Y

S

H

P

P

G

G

D

D

V

I

Y

Y

W

W

C

C

T

T

A

A

D

D

V

V

G

G

W

W

I

V

T

T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

L

A

A

C

G

G

A

A

T

T

I

L

M

M

F

F

E

E

G

G

V

V

P

P

T

N

F

W

P

P

D

T

C

P

G

A

R

R

F

M

W

C

A

Q

I

V

V

V

E

D

K

K

Y

H

K

Q

V

V

N

N

Q

I

F

L

Y

Y

T

T

A

A

P

P

T

T

A

A

I

I

R

R

A

A

L

L

Q

M

A

A

Y

E

G

G

T

D

V

K

E

A

I

I

E

E

K

G

Y

T

D

D

L

R

S

S

L

L

K

R

V

I

L

L

G

G

S

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

E

P

E

E

A

A

W

W

H

E

W

W

Y

Y

H

W

T

K

H

K

I

I

G

G

K

K

N

E

K

K

C

C

P

P

I

V

V

V

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

I

F

M

M

V

I

S

T

P

P

I

L

A

P

G

G

I

A

T

I

P

E

T

L

K

K

P

A

A

G

Y

S

A

A

T

T

L

R

P

P

L

F

P

F

G

G

V

V

Q

Q

L

P

C

A

I

L

V

V

D

D

P

N

E

E

G

G

N

H

E

P

L

Q

T

E

G

G

N

-

S

A

V

T

E

E

G

G

N

N

L

L

C

V

I

I

K

T

F

D

P

S

W

W

P

P

S

G

M

Q

I

A

R

R

T

T

T

L

Y

F

G

G

D

D

H

H

D

E

R

R

C

F

K

E

Q

Q

T

T

Y

Y

F

F

S

S

T

T

Y

F

K

K

G

N

M

M

Y

Y

F

F

T

S

G

G

D
|
D

G

G

V

A

K

R

R

R

D

D

H

E

D

D

G

G

Y

Y

R

W

I
|
I

L

T

G

G

R

R

V

V

D

D

V

V

I

L

N
|
N

V

V

S
|
S

G

G

H

H

R
|
R

M

L

G

G

T

T

A

A

E

E

V

I

E

E

N

S

A

A

I

L

N
x
V

E

A

H
|
H

P

P

K

K

V
x
I

I

A

E

E

S

A

A

A

V

V

G

G

Y

I

P

P

H

H

D

A

V

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

A

A

Y

Y

V

V

I

T

C

L

D

N

L

H

T

G

N

E

R

E

T

P

E

S

D

P

N

E

L

L

V

Y

N

A

E

E

I

V

K

R

E

N

M

W

V

V

S
x
R

K

K

I

E

I

I

G

G

P
|
P

I

L

A

A

K

T

P

P

D

D

K

V

I

L

Q

H

L

W

V

T

P

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

V

I

A

A

E

A

N

G

N

D

L

L

D

-

N

-

M

-

G

G

D

D

T

T

S

S

T

T

L

L

L

A

D

D

P

P

D

G

V

V

E

E

K

K

I

L

D

E

G

E

R

K

K

Q

active site: T260 (= T247), T412 (= T399), E413 (= E400), N517 (= N505), R522 (= R510), K605 (= K593)
binding (r,r)-2,3-butanediol: W93 (= W82), E140 (= E130), G169 (≠ D159), K266 (= K253), P267 (= P254)
binding adenosine-3',5'-cyclic-monophosphate: G383 (= G370), E384 (= E371), P385 (= P372), T408 (= T395), W409 (= W396), W410 (= W397), Q411 (= Q398), T412 (= T399), D496 (= D484), I508 (= I496), N517 (= N505), R522 (= R510)
binding coenzyme a: F159 (= F149), G160 (≠ A150), G161 (= G151), R187 (= R177), S519 (= S507), R580 (≠ S568), P585 (= P573)
binding magnesium ion: V533 (≠ N521), H535 (= H523), I538 (≠ V526)

2p20A Acetyl-coa synthetase, r584a mutation (see paper)
55% identity, 98% coverage: 11:630/630 of query aligns to 20:640/641 of 2p20A

query
sites
2p20A

Y

Y

L

E

H

T

E

K

Y

Y

Q

K

K

Q

S

S

V

I

A

N

Q

D

P

P

E

D

E

T

F

F

W

W

A

G

R

E

I

Q

A

G

D

K

S

I

F

L

H

D

W

W

R

I

K

T

R

P

W

Y

D

Q

K

K

T

V

L

K

K

N

W

T

N

S

F

F

E

A

G

P

P

G

D

N

V

V

-

S

-

I

K

K

W

W

F

Y

L

E

N

D

G

G

K

T

L

L

N

N

I

L

T

A

E

A

N

N

I

C

F

L

E

D

R

R

Y

H

L

L

F

Q

T

E

I

N

G

G

D

D

R

R

P

T

A

A

I

I

W

W

E

E

P

G

N

D

D

D

P

T

N

S

E

Q

A

S

G

-

R

K

T

H

L

I

T

S

Y

Y

R

R

E

E

L

L

F

H

E

R

E

D

V

V

S

C

R

R

F

F

S

A

N

N

A

T

L

L

K

L

S

D

K

L

G

G

I

I

G

K

K

K

G

G

D

D

K

V

V

V

I

A

I

I

Y

Y

M

M

P

P

M

M

V

V

P

P

E

E

A

A

V

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

I

V

H

H

S

S

V

V

V

I

F

F

A

G

G

G

F

F

S

S

S

P

N

E

A

A

L

V

A

A

D

G

R

R

I

I

N

I

D

D

C

S

E

S

A

S

K

R

A

L

V

V

L

I

T

T

S

A

D

D

G

E

N

G

F

V

R

R

G

A

S

G

K

R

K

S

I

I

A

P

V

L

K

K

A

K

V

N

V

V

D

D

E

D

A

A

L

L

T

K

K

N

-

P

-

N

-

V

S

T

S

S

V

V

E

E

T

H

V

V

I

I

V

V

Y

L

Q

K

R

R

T

T

H

G

Q

S

D

D

V

I

T

D

M

W

Q

Q

D

E

G

G

R

R

D

D

Y

L

W

W

H

R

D

D

V

L

V

I

A

E

D

K

E

A

S

S

K

P

D

E

C

H

P

Q

A

P

E

E

E

A

M

M

D

N

S

A

E

E

D

D

M

P

L

L

F

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

K

K

P

P

K

K

G

G

V

V

V

L

H

H

T

T

G

G

Y

Y

M

L

V

V

Y

Y

S

A

K

A

Y

T

S

T

F

F

E

K

N

Y

V

V

F

F

Q

D

Y

Y

S

H

P

P

G

G

D

D

V

I

Y

Y

W

W

C

C

T

T

A

A

D

D

V

V

G

G

W

W

I

V

T

T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

L

A

A

C

G

G

A

A

T

T

I

L

M

M

F

F

E

E

G

G

V

V

P

P

T

N

F

W

P

P

D

T

C

P

G

A

R

R

F

M

W

C

A

Q

I

V

V

V

E

D

K

K

Y

H

K

Q

V

V

N

N

Q

I

F

L

Y

Y

T

T

A

A

P

P

T

T

A

A

I

I

R

R

A

A

L

L

Q

M

A

A

Y

E

G

G

T

D

V

K

E

A

I

I

E

E

K

G

Y

T

D

D

L

R

S

S

L

L

K

R

V

I

L

L

G

G

S

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

E

P

E

E

A

A

W

W

H

E

W

W

Y

Y

H

W

T

K

H

K

I

I

G

G

K

K

N

E

K

K

C

C

P

P

I

V

V

V

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

I

F

M

M

V

I

S

T

P

P

I

L

A

P

G

G

I

A

T

I

P

E

T

L

K

K

P

A

A

G

Y

S

A

A

T

T

L

R

P

P

L

F

P

F

G

G

V

V

Q

Q

L

P

C

A

I

L

V

V

D

D

P

N

E

E

G

G

N

H

E

P

L

Q

T

E

G

G

N

-

S

A

V

T

E

E

G

G

N

N

L

L

C

V

I

I

K

T

F

D

P

S

W

W

P

P

S

G

M

Q

I

A

R

R

T

T

T

L

Y

F

G

G

D

D

H

H

D

E

R

R

C

F

K

E

Q

Q

T

T

Y

Y

F

F

S

S

T

T

Y

F

K

K

G

N

M

M

Y

Y

F

F

T

S

G

G

D
|
D

G

G

V

A

K

R

R

R

D

D

H

E

D

D

G

G

Y

Y

R

W

I
|
I

L

T

G

G

R
|
R

V

V

D

D

V

V

I

L

N
|
N

V

V

S

S

G

G

H

H

R
|
R

M

L

G

G

T

T

A

A

E

E

V

I

E

E

N

S

A

A

I

L

N

V

E

A

H

H

P

P

K

K

V

I

I

A

E

E

S

A

A

A

V

V

G

G

Y

I

P

P

H

H

D

A

V

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

A

A

Y

Y

V

V

I

T

C

L

D

N

L

H

T

G

N

E

R

E

T

P

E

S

D

P

N

E

L

L

V

Y

N

A

E

E

I

V

K

R

E

N

M

W

V

V

S

A

K

K

I

E

I

I

G

G

P

P

I

L

A

A

K

T

P

P

D

D

K

V

I

L

Q

H

L

W

V

T

P

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

V

I

A

A

E

A

N

G

N

D

L

T

D

S

N

N

M

-

G

-

D

D

T

T

S

S

T

T

L

L

L

A

D

D

P

P

D

G

V

V

E

E

K

K

I

L

D

E

G

E

R

K

K

Q

active site: T260 (= T247), T412 (= T399), E413 (= E400), N517 (= N505), R522 (= R510), K605 (= K593)
binding adenosine-5'-monophosphate-propyl ester: G383 (= G370), E384 (= E371), P385 (= P372), T408 (= T395), W409 (= W396), W410 (= W397), Q411 (= Q398), T412 (= T399), D496 (= D484), I508 (= I496), R511 (= R499), R522 (= R510)

2p2fA Acetyl-coa synthetase, wild-type with acetate, amp, and coa bound (see paper)
55% identity, 98% coverage: 11:630/630 of query aligns to 19:636/637 of 2p2fA

query
sites
2p2fA

Y

Y

L

E

H

T

E

K

Y

Y

Q

K

K

Q

S

S

V

I

A

N

Q

D

P

P

E

D

E

T

F

F

W

W

A

G

R

E

I

Q

A

G

D

K

S

I

F

L

H

D

W

W

R

I

K

T

R

P

W

Y

D

Q

K

K

T

V

L

K

K

N

W

T

N

S

F

F

E

A

G

P

P

G

D

N

V

V

-

S

-

I

K

K

W

W

F

Y

L

E

N

D

G

G

K

T

L

L

N

N

I

L

T

A

E

A

N

N

I

C

F

L

E

D

R

R

Y

H

L

L

F

Q

T

E

I

N

G

G

D

D

R

R

P

T

A

A

I

I

W

W

E

E

P

G

N

D

D

D

P

T

N

S

E

Q

A

S

G

-

R

K

T

H

L

I

T

S

Y

Y

R

R

E

E

L

L

F

H

E

R

E

D

V

V

S

C

R

R

F

F

S

A

N

N

A

T

L

L

K

L

S

D

K

L

G

G

I

I

G

K

K

K

G

G

D

D

K

V

V

V

I

A

I

I

Y

Y

M

M

P

P

M

M

V

V

P

P

E

E

A

A

V

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

I

V

H

H

S

S

V

V

V

I

F
|
F

A

G

G

G

F

F

S

S

S

P

N

E

A

A

L

V

A

A

D

G

R

R

I

I

N

I

D

D

C

S

E

S

A

S

K

R

A

L

V

V

L

I

T

T

S

A

D

D

G

E

N

G

F

V

R
|
R

G

A

S

G

K

R

K

S

I

I

A

P

V

L

K

K

A

K

V

N

V

V

D

D

E

D

A

A

L

L

T

K

K

N

-

P

-

N

-

V

S

T

S

S

V

V

E

E

T

H

V

V

I

I

V

V

Y

L

Q

K

R

R

T

T

H

G

Q

S

D

D

V

I

T

D

M

W

Q

Q

D

E

G

G

R

R

D

D

Y

L

W

W

H

R

D

D

V

L

V

I

A

E

D

K

E

A

S

S

K

P

D

E

C

H

P

Q

A

P

E

E

E

A

M

M

D

N

S

A

E

E

D

D

M

P

L

L

F

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

K

K

P

P

K

K

G

G

V

V

V

L

H

H

T

T

G

G

Y

Y

M

L

V

V

Y

Y

S

A

K

A

Y

T

S

T

F

F

E

K

N

Y

V

V

F

F

Q

D

Y

Y

S

H

P

P

G

G

D

D

V

I

Y

Y

W

W

C

C

T

T

A

A

D

D

V

V

G

G

W
|
W

I

V

T
|
T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

L

A

A

C

G

G

A

A

T

T

I

L

M

M

F

F

E

E

G

G

V

V

P
|
P

T

N

F

W

P

P

D

T

C

P

G

A

R

R

F

M

W

C

A

Q

I

V

V

V

E

D

K

K

Y

H

K

Q

V

V

N

N

Q

I

F

L

Y

Y

T

T

A
|
A

P

P

T

T

A
|
A

I

I

R

R

A

A

L

L

Q

M

A

A

Y

E

G

G

T

D

V

K

E

A

I

I

E

E

K

G

Y

T

D

D

L

R

S

S

L

L

K

R

V

I

L

L

G

G

S

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

E

P

E

E

A

A

W

W

H

E

W

W

Y

Y

H

W

T

K

H

K

I

I

G

G

K

K

N

E

K

K

C

C

P

P

I

V

V

V

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

I

F

M

M

V

I

S

T

P

P

I

L

A

P

G

G

I

A

T

I

P

E

T

L

K

K

P

A

A

G

Y

S

A

A

T

T

L

R

P

P

L

F

P

F

G

G

V

V

Q

Q

L

P

C

A

I

L

V

V

D

D

P

N

E

E

G

G

N

H

E

P

L

Q

T

E

G

G

N

-

S

A

V

T

E

E

G

G

N

N

L

L

C

V

I

I

K

T

F

D

P

S

W

W

P

P

S

G

M

Q

I

A

R

R

T

T

T

L

Y

F

G

G

D

D

H

H

D

E

R

R

C

F

K

E

Q

Q

T

T

Y

Y

F

F

S

S

T

T

Y

F

K

K

G

N

M

M

Y

Y

F

F

T

S

G

G

D
|
D

G

G

V

A

K

R

R

R

D

D

H

E

D

D

G

G

Y

Y

R

W

I
|
I

L

T

G

G

R
|
R

V

V

D

D

V

V

I

L

N
|
N

V

V

S
|
S

G

G

H

H

R
|
R

M

L

G

G

T

T

A

A

E

E

V

I

E

E

N

S

A

A

I

L

N

V

E

A

H

H

P

P

K

K

V

I

I

A

E

E

S

A

A

A

V

V

G

G

Y

I

P

P

H

H

D

A

V

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

A

A

Y

Y

V

V

I

T

C

L

D

N

L

H

T

G

N

E

R

E

T

P

E

S

D

P

N

E

L

L

V

Y

N

A

E

E

I

V

K

R

E

N

M

W

V

V

S
x
R

K

K

I

E

I

I

G

G

P
|
P

I

L

A

A

K

T

P

P

D

D

K

V

I

L

Q

H

L

W

V

T

P

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

V

I

A

A

E

-

N

-

L

-

D

-

N

-

M

A

G

G

D

D

T

T

-

S

S

S

T

T

L

L

L

A

D

D

P

P

D

G

V

V

E

E

K

K

I

L

D

E

G

E

R

K

K

Q

active site: T259 (= T247), T411 (= T399), E412 (= E400), N516 (= N505), R521 (= R510), K604 (= K593)
binding adenosine monophosphate: G382 (= G370), E383 (= E371), P384 (= P372), T407 (= T395), W408 (= W396), W409 (= W397), Q410 (= Q398), T411 (= T399), D495 (= D484), I507 (= I496), R510 (= R499), N516 (= N505), R521 (= R510)
binding coenzyme a: F158 (= F149), R186 (= R177), W304 (= W292), T306 (= T294), P329 (= P317), A352 (= A340), A355 (= A343), S518 (= S507), R579 (≠ S568), P584 (= P573)

1pg3A Acetyl coa synthetase, acetylated on lys609 (see paper)
54% identity, 98% coverage: 11:630/630 of query aligns to 20:633/634 of 1pg3A

query
sites
1pg3A

Y

Y

L

E

H

T

E

K

Y

Y

Q

K

K

Q

S

S

V

I

A

N

Q

D

P

P

E

D

E

T

F

F

W

W

A

G

R

E

I

Q

A

G

D

K

S

I

F

L

H

D

W

W

R

I

K

T

R

P

W

Y

D

Q

K

K

T

V

L

K

K

N

W

T

N

S

F

F

E

A

G

P

P

G

D

N

V

V

-

S

-

I

K

K

W

W

F

Y

L

E

N

D

G

G

K

T

L

L

N

N

I

L

T

A

E

A

N

N

I

C

F

L

E

D

R

R

Y

H

L

L

F

Q

T

E

I

N

G

G

D

D

R

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active site: T260 (= T247), T412 (= T399), E413 (= E400), N517 (= N505), R522 (= R510), K605 (= K593)
binding coenzyme a: F159 (= F149), G160 (≠ A150), R187 (= R177), R190 (≠ K180), A301 (= A288), T307 (= T294), P330 (= P317), A356 (= A343), S519 (= S507), R580 (≠ S568), P585 (= P573)
binding magnesium ion: V533 (≠ N521), H535 (= H523), I538 (≠ V526)
binding adenosine-5'-monophosphate-propyl ester: G383 (= G370), E384 (= E371), P385 (= P372), T408 (= T395), W409 (= W396), W410 (= W397), Q411 (= Q398), T412 (= T399), D496 (= D484), R511 (= R499), R522 (= R510)

P52910 Acetyl-coenzyme A synthetase 2; Acetate--CoA ligase 2; Acyl-activating enzyme 2; EC 6.2.1.1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
50% identity, 98% coverage: 8:626/630 of query aligns to 37:670/683 of P52910

query
sites
P52910

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