SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_008198582.1 NCBI__GCF_000166275.1:WP_008198582.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6uziC Crystal structure of dihydrolipoyl dehydrogenase from elizabethkingia anophelis nuhp1
74% identity, 100% coverage: 2:466/466 of query aligns to 6:470/470 of 6uziC

query
sites
6uziC

N

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F

D

D

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I

G
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Y

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A

A

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A

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Y

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C

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A

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M

active site: C45 (= C41), C50 (= C46), S53 (= S49), V187 (= V183), E191 (= E187), H448 (= H444), E453 (= E449)
binding flavin-adenine dinucleotide: I12 (= I8), G13 (= G9), G15 (= G11), P16 (= P12), G17 (= G13), E36 (= E32), K37 (= K33), G43 (= G39), T44 (= T40), C45 (= C41), G49 (= G45), C50 (= C46), S53 (= S49), K54 (= K50), V117 (= V113), G118 (= G114), T147 (= T143), G148 (= G144), I188 (= I184), R276 (= R272), D316 (= D312), M322 (= M318), L323 (= L319), A324 (= A320)
binding zinc ion: H448 (= H444), E453 (= E449)

3urhB Crystal structure of a dihydrolipoamide dehydrogenase from sinorhizobium meliloti 1021
53% identity, 98% coverage: 3:457/466 of query aligns to 1:458/465 of 3urhB

query
sites
3urhB

Y

Y

D

D

V

L

I

I

V

V

I
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I

G
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G

S

S

G
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G

P
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P

G
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G

G

G

Y

Y

V

V

A

C

A

A

I

I

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C

A

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A

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Q

L

L

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G

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M

K

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T

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A

A

I

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V

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E
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E

K
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Y

R

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S

T

T

L
x
Y

G

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C
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C

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L

N

N

V

V

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G

C
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C

I

I

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S

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K

A

A

L

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L

L

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H

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A

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E

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F

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H

N

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F

L

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E

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A

H

L

G

G

I

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L

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N

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P

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N

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M

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M

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A

R

H

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N

D

D

A

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D

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F

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F

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N

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A

A

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M

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D

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D

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A

A

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A

K

E

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L

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G

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H

H

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I

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F

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A

A

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E

M

M

I

I

A

H

E

E

A

I

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E

E

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F

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G

A

G

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S

A

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E

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I

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A

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A

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K

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E

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A

L

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A

S

active site: Y35 (≠ L37), C39 (= C41), C44 (= C46), S47 (= S49), V183 (= V183), E187 (= E187), H443 (= H442), H445 (= H444), E450 (= E449)
binding flavin-adenine dinucleotide: I6 (= I8), G7 (= G9), G9 (= G11), P10 (= P12), G11 (= G13), E30 (= E32), K31 (= K33), G37 (= G39), T38 (= T40), C39 (= C41), G43 (= G45), C44 (= C46), K48 (= K50), T111 (≠ V113), G112 (= G114), A140 (= A142), T141 (= T143), G142 (= G144), I184 (= I184), R273 (= R272), G312 (= G311), D313 (= D312), M319 (= M318), L320 (= L319), A321 (= A320), H322 (= H321)

6aonA 1.72 angstrom resolution crystal structure of 2-oxoglutarate dehydrogenase complex subunit dihydrolipoamide dehydrogenase from bordetella pertussis in complex with fad
52% identity, 100% coverage: 2:466/466 of query aligns to 2:473/473 of 6aonA

query
sites
6aonA

N

Q

Y

F

D

D

V

V

I

V

V

V

I
|
I

G

G

S

A

G
|
G

P
|
P

G
|
G

G

G

Y

Y

V

I

A

A

A

A

I

I

R

R

C

A

A

A

Q

Q

L

L

G

G

M

M

K

S

T

V

A

A

I

C

V

I

E
x
D

K
x
A

Y
x
W

-

Q

-

N

-

G

-

Q

-

G

-

G

P

P

T

A

L
x
P

G

G

G
|
G

T
|
T

C
|
C

L

T

N

N

V

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

A

A

L

L

L

L

D

Q

S

S

S

S

E

E

H

H

Y

Y

H

E

N

Q

A

A

A

N

H

H

T

H

F

F

K

A

T

E

H

H

G

G

I

I

N

E

L

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N

G

L

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S

V

L

D

K

L

L

K

D

Q

T

M

L

I

I

T

G

R

R

K

K

N

N

D

T

V

V

V

V

K

K

Q

Q

N

N

T

N

D

D

G

G

I

I

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L

Y

Y

L

L

M

F

K

K

K

K

N

N

K

K

I

V

E

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Y

H

F

Q

H

G

G

V
x
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G
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S

A

F

F

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A

D

G

K

Q

T

V

-

D

-

G

-

G

-

W

T

S

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K

V

V

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T

K

G

D

T

D

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G

-

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D

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A

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x
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A

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A

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R

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L

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A

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I

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M

M

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E

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x
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T

A

M
x
A

D

D

R
x
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T

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A

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K

E

E

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A

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A

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A

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E

V

Q

E

K

I

L

K

V

G

V

D

D

Y

K

V

L

L

I

V

V

S

S

I

I

G

G

R
|
R

R

V

P

P

Y
|
Y

T

T

D

G

G

G

L

L

N

N

A

A

E

E

A

A

A

V

G

G

L

L

K

K

L

L

T

D

D

E

R

R

G

G

Q

F

I

V

E

A

V

V

N

D

D

E

H

D

L

C

Q

K

T

T

N

N

V

L

P

P

N

N

I

V

Y

W

A

A

I

V

G
|
G

D
|
D

V

V

V

V

K

R

G

G

A

P

M
|
M

L
|
L

A
|
A

H
|
H

K

K

A

A

E

E

E

E

E

E

G

G

V

V

F

A

V

V

A

A

E

E

T

R

L

I

V

A

G

G

Q

Q

K

H

P

G

H

H

I

V

N

N

Y

F

N

A

L

T

I

V

P

P

G

W

V

V

V

I

Y

Y

T

T

W

S

P

P

E

E

V

I

A

A

A

W

V

V

G

G

Y

K

T

T

E

E

E

Q

Q

Q

L

L

K

K

E

A

K

E

G

G

I

R

K

E

Y

Y

K

K

A

A

G

G

K

S

F

F

P

P

F

F

M

M

A

A

S

N

G

G

R

R

A

A

R

R

A

A

S

L

M

G

D

D

T

T

D

T

G

G

L

F

V

A

K

K

V

V

L

I

A

A

D

D

A

A

E

K

T

T

D

D

E

E

I

V

L

L

G

G

V

V

H

H

M

I

I

I

G

G

P

P

R

M

T

A

A

S

D

E

M

L

I

I

A

S

E

E

A

A

V

V

V

T

A

I

M

M

E

E

Y

F

R

R

A

G

S

A

A

A

E

E

D

D

I

I

A

A

R

R

M

I

S

C

H
|
H

A

A

H
|
H

P

P

T

T

Y

L

T

S

E
|
E

A

A

F

V

K

K

E

E

A

A

C

A

L

L

A

-

A

A

T

V

D

D

N

K

R

R

A

T

L

L

H

N

I

F

active site: P43 (≠ L37), C47 (= C41), C52 (= C46), S55 (= S49), V191 (= V183), E195 (= E187), H450 (= H442), H452 (= H444), E457 (= E449)
binding calcium ion: A218 (≠ P210), A220 (≠ M212), Q222 (≠ R214)
binding flavin-adenine dinucleotide: I8 (= I8), G11 (= G11), P12 (= P12), G13 (= G13), D32 (≠ E32), A33 (≠ K33), W34 (≠ Y34), G45 (= G39), T46 (= T40), C47 (= C41), G51 (= G45), C52 (= C46), K56 (= K50), K119 (≠ V113), G120 (= G114), T151 (= T143), G152 (= G144), N171 (≠ S163), I192 (= I184), R280 (= R272), Y283 (= Y275), G319 (= G311), D320 (= D312), M326 (= M318), L327 (= L319), A328 (= A320), H329 (= H321)

P18925 Dihydrolipoyl dehydrogenase; Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex; EC 1.8.1.4 from Azotobacter vinelandii (see 2 papers)
51% identity, 100% coverage: 3:466/466 of query aligns to 5:472/477 of P18925

query
sites
P18925

Y

F

D

D

V

V

I

I

V

V

I

I

G

G

S

A

G

G

P

P

G

G

G

G

Y

Y

V

V

A

A

A

A

I

I

R

K

C

S

A

A

Q

Q

L

L

G

G

M

L

K

K

T

T

A

A

I

L

V

I

E
|
E

K
|
K

Y
|
Y

-
x
K

-
x
G

-
x
K

-
x
E

-
x
G

-
x
K

P
x
T

T
x
A

L
|
L

G
|
G

G
|
G

T
|
T

C
|
C

L

L

N

N

V

V

G

G

C
|
C

I

I

P

P

S

S

K
|
K

A

A

L

L

L

L

D

D

S

S

S

S

E

Y

H

K

Y

F

H

H

N

E

A

A

A

H

H

E

T

S

F

F

K

K

T

L

H

H

G

G

I

I

N

S

L

T

S

G

N

E

L

V

K

A

V

I

D

D

L

V

K

P

Q

T

M

M

I

I

T

A

R

R

K

K

N

D

D

Q

V

I

V

V

K

R

Q

N

N

L

T

T

D

G

G

G

I

V

Q

A

Y

S

L

L

M

I

K

K

K

A

N

N

K

G

I

V

E

T

V

L

H

F

Q

E

G

G

V

H

G

G

S

K

F

L

V

L

D

A

K

G

T

K

T

K

V

V

K

E

V

V

T

T

K

A

D

A

D

D

G

G

S

S

S

S

S

Q

D

V

I

L

Q

D

G

T

K

E

N

N

I

V

I

I

V

L

A

A

T

S

G

G

S

S

K

K

P

P

S

V

N

E

L

I

P

P

F

P

I

A

K

P

L

V

D

D

K

Q

D

D

R

V

V

I

I

V

T

D

S

S

T

T

E

G

A

A

L

L

N

D

L

F

K

Q

E

N

T

V

P

P

K

G

H

K

L

L

V

G

V

V

I

I

G

G

G

A

G

G

V

V

I

I

G

G

L

L

E

E

L

L

G

G

S

S

V

V

Y

W

A

A

R

R

L

L

G

G

A

A

K

E

V

V

S

T

V

V

I

L

E

E

F

A

M

M

D

D

S

K

L

F

I

L

P

P

T

A

M

V

D

D

R

E

T

Q

M

V

G

A

K

K

E

E

L

A

Q

Q

K

K

S

I

L

L

K

T

K

K

I

Q

G

G

F

L

E

K

F

I

F

L

L

L

K

G

H

A

K

R

V

V

T

T

A

G

V

T

E

E

K

V

K

K

G

N

K

K

E

Q

V

V

T

T

V

V

K

K

A

F

D

V

N

D

A

A

K

E

G

G

E

E

T

K

V

S

E

Q

I

-

K

A

G

F

D

D

Y

K

V

L

L

I

V

V

S

A

I

V

G

G

R

R

R

R

P

P

Y

V

T

T

D

T

G

D

L

L

N

L

A

A

E

A

A

D

A

S

G

G

L

V

K

T

L

L

T

D

D

E

R

R

G

G

Q

F

I

I

E

Y

V

V

N

D

D

D

H

Y

L

C

Q

A

T

T

N

S

V

V

P

P

N

G

I

V

Y

Y

A

A

I

I

G

G

D
|
D

V

V

V

V

K

R

G

G

A

A

M

M

L

L

A
|
A

H

H

K

K

A

A

E

S

E

E

E

E

G

G

V

V

F

V

V

V

A

A

E

E

T

R

L

I

V

A

G

G

Q

H

K

K

P

A

H

Q

I

M

N

N

Y

Y

N

D

L

L

I

I

P

P

G

A

V

V

V

I

Y

Y

T

T

W

H

P

P

E

E

V

I

A

A

A

G

V

V

G

G

Y

K

T

T

E

E

E

Q

Q

A

L

L

K

K

E

A

K

E

G

G

I

V

K

A

Y

I

K

N

A

V

G

G

K

V

F

F

P

P

F

F

M

A

A

A

S

S

G

G

R

R

A

A

R

M

A

A

S

A

M

N

D

D

T

T

D

A

G

G

L

F

V

V

K

K

V

V

L

I

A

A

D

D

A

A

E

K

T

T

D

D

E

R

I

V

L

L

G

G

V

V

H

H

M

V

I

I

G

G

P

P

R

S

T

A

A

A

D

E

M

L

I

V

A

Q

E

Q

A

G

V

A

V

I

A

A

M

M

E

E

Y

F

R

G

A

T

S

S

A

A

E

E

D

D

I

L

A

G

R

M

M

M

S

V

H

F

A

A

H

H

P

P

T

A

Y

L

T

S

E

E

A

A

F

L

K

H

E

E

A

A

C

A

L

L

A

A

A

V

T

S

D

-

N

G

R

H

A

A

L

I

H

H

I

V

34:49 (vs. 32:41, 50% identical) binding FAD
C49 (= C41) modified: Disulfide link with 54, Redox-active
C54 (= C46) modified: Disulfide link with 49, Redox-active
K58 (= K50) binding FAD
D319 (= D312) binding FAD
A327 (= A320) binding FAD

3ladA Refined crystal structure of lipoamide dehydrogenase from azotobacter vinelandii at 2.2 angstroms resolution. A comparison with the structure of glutathione reductase (see paper)
51% identity, 100% coverage: 3:466/466 of query aligns to 4:471/472 of 3ladA

query
sites
3ladA

Y

F

D

D

V

V

I

I

V

V

I
|
I

G
|
G

S

A

G
|
G

P
|
P

G

G

G

G

Y

Y

V

V

A

A

A

A

I

I

R

K

C

S

A

A

Q

Q

L

L

G

G

M

L

K

K

T

T

A

A

I

L

V

I

E
|
E

K
|
K

Y

Y

-

K

-

G

-

K

-

E

-

G

-

K

P

T

T

A

L
|
L

G

G

G
|
G

T
|
T

C
|
C

L

L

N

N

V

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K

K

A

A

L

L

L

L

D

D

S

S

S

S

E

Y

H

K

Y

F

H

H

N

E

A

A

A

H

H

E

T

S

F

F

K

K

T

L

H

H

G

G

I

I

N

S

L

T

S

G

N

E

L

V

K

A

V

I

D

D

L

V

K

P

Q

T

M

M

I

I

T

A

R

R

K

K

N

D

D

Q

V

I

V

V

K

R

Q

N

N

L

T

T

D

G

G

G

I

V

Q

A

Y

S

L

L

M

I

K

K

K

A

N

N

K

G

I

V

E

T

V

L

H

F

Q

E

G

G

V
x
H

G
|
G

S

K

F

L

V

L

D

A

K

G

T

K

T

K

V

V

K

E

V

V

T

T

K

A

D

A

D

D

G

G

S

S

S

S

S

Q

D

V

I

L

Q

D

G

T

K

E

N

N

I

V

I

I

V

L

A
|
A

T
x
S

G
|
G

S

S

K

K

P

P

S

V

N

E

L

I

P

P

F

P

I

A

K

P

L

V

D

D

K

Q

D

D

R

V

V

I

I

V

T

D

S

S

T

T

E

G

A

A

L

L

N

D

L

F

K

Q

E

N

T

V

P

P

K

G

H

K

L

L

V

G

V

V

I

I

G

G

G

A

G

G

V
|
V

I
|
I

G

G

L

L

E
|
E

L

L

G

G

S

S

V

V

Y

W

A

A

R

R

L

L

G

G

A

A

K

E

V

V

S

T

V

V

I

L

E

E

F

A

M

M

D

D

S

K

L

F

I

L

P

P

T

A

M

V

D

D

R

E

T

Q

M

V

G

A

K

K

E

E

L

A

Q

Q

K

K

S

I

L

L

K

T

K

K

I

Q

G

G

F

L

E

K

F

I

F

L

L

L

K

G

H

A

K

R

V

V

T

T

A

G

V

T

E

E

K

V

K

K

G

N

K

K

E

Q

V

V

T

T

V

V

K

K

A

F

D

V

N

D

A

A

K

E

G

G

E

E

T

K

V

S

E

Q

I

A

K

F

G

-

D

D

Y

K

V

L

L

I

V

V

S

A

I

V

G

G

R
|
R

R

R

P

P

Y

V

T

T

D

T

G

D

L

L

N

L

A

A

E

A

A

D

A

S

G

G

L

V

K

T

L

L

T

D

D

E

R

R

G

G

Q

F

I

I

E

Y

V

V

N

D

D

D

H

Y

L

C

Q

A

T

T

N

S

V

V

P

P

N

G

I

V

Y

Y

A

A

I

I

G

G

D
|
D

V

V

V

V

K

R

G

G

A

A

M

M

L
|
L

A
|
A

H

H

K

K

A

A

E

S

E

E

E

E

G

G

V

V

F

V

V

V

A

A

E

E

T

R

L

I

V

A

G

G

Q

H

K

K

P

A

H

Q

I

M

N

N

Y

Y

N

D

L

L

I

I

P

P

G

A

V

V

V

I

Y

Y

T

T

W

H

P

P

E

E

V

I

A

A

A

G

V

V

G

G

Y

K

T

T

E

E

E

Q

Q

A

L

L

K

K

E

A

K

E

G

G

I

V

K

A

Y

I

K

N

A

V

G

G

K

V

F

F

P

P

F

F

M

A

A

A

S

S

G

G

R

R

A

A

R

M

A

A

S

A

M

N

D

D

T

T

D

A

G

G

L

F

V

V

K

K

V

V

L

I

A

A

D

D

A

A

E

K

T

T

D

D

E

R

I

V

L

L

G

G

V

V

H

H

M

V

I

I

G

G

P

P

R

S

T

A

A

A

D

E

M

L

I

V

A

Q

E

Q

A

G

V

A

V

I

A

A

M

M

E

E

Y

F

R

G

A

T

S

S

A

A

E

E

D

D

I

L

A

G

R

M

M

M

S

V

H
x
F

A

A

H
|
H

P

P

T

A

Y

L

T

S

E
|
E

A

A

F

L

K

H

E

E

A

A

C

A

L

L

A

A

A

V

T

S

D

-

N

G

R

H

A

A

L

I

H

H

I

V

active site: L44 (= L37), C48 (= C41), C53 (= C46), S56 (= S49), V190 (= V183), E194 (= E187), F448 (≠ H442), H450 (= H444), E455 (= E449)
binding flavin-adenine dinucleotide: I9 (= I8), G10 (= G9), G12 (= G11), P13 (= P12), E33 (= E32), K34 (= K33), G46 (= G39), T47 (= T40), C48 (= C41), G52 (= G45), C53 (= C46), H120 (≠ V113), G121 (= G114), A149 (= A142), S150 (≠ T143), G151 (= G144), I191 (= I184), R278 (= R272), D318 (= D312), L325 (= L319), A326 (= A320)

P31023 Dihydrolipoyl dehydrogenase, mitochondrial; Dihydrolipoamide dehydrogenase; Glycine cleavage system L protein; Pyruvate dehydrogenase complex E3 subunit; E3; PDC-E3; EC 1.8.1.4 from Pisum sativum (Garden pea) (Lathyrus oleraceus) (see 2 papers)
52% identity, 99% coverage: 4:466/466 of query aligns to 39:501/501 of P31023

query
sites
P31023

D

D

V

V

I

V

V

I

I

I

G

G

S

G

G

G

P

P

G

G

G

G

Y

Y

V

V

A

A

A

A

I

I

R

K

C

A

A

A

Q

Q

L

L

G

G

M

F

K

K

T

T

A

T

I

C

V

I

E
|
E

K
|
K

Y
x
R

P
x
G

T
x
A

L
|
L

G
|
G

G
|
G

T
|
T

C
|
C

L

L

N

N

V

V

G

G

C
|
C

I

I

P

P

S

S

K

K

A

A

L

L

L

L

D

H

S

S

S

S

E

H

H

M

Y

Y

H

H

N

E

A

A

A

K

H

H

T

S

F

F

K

A

T

N

H

H

G

G

I

V

N

K

L

V

S

S

N

N

L

V

K

E

V

I

D

D

L

L

K

A

Q

A

M

M

I

M

T

G

R

Q

K

K

N

D

D

K

V

A

V

V

K

S

Q

N

N

L

T

T

D

R

G

G

I

I

Q

E

Y

G

L

L

M

F

K

K

K

K

N

N

K

K

I

V

E

T

V

Y

H

V

Q

K

G

G

V

Y

G
|
G

S

K

F

F

V

V

D

S

K

P

T

S

T

E

V

I

K

S

V

V

T

D

K

T

D

I

D

E

G

G

S

E

S

N

S

T

D

V

I

V

Q

K

G

G

K

K

N

H

I

I

I

I

V

I

A

A

T

T

G

G

S

S

K

D

P

V

S

K

N

S

L

L

P

P

F

G

I

V

K

T

L

I

D

D

K

E

D

K

R

K

V

I

I

V

T

S

S

S

T

T

E

G

A

A

L

L

N

A

L

L

K

S

E

E

T

I

P

P

K

K

H

K

L

L

V

V

V

V

I

I

G

G

G

A

G

G

V

Y

I

I

G

G

L

L

E

E

L

M

G

G

S

S

V

V

Y

W

A

G

R

R

L

I

G

G

A

S

K

E

V

V

S

T

V

V

I

V

E

E

F

F

M

A

D

S

S

E

L

I

I

V

P

P

T

T

M

M

D

D

R

A

T

E

M

I

G

R

K

K

E

Q

L

F

Q

Q

K

R

S

S

L

L

K

E

K

K

I

Q

G

G

F

M

E

K

F

F

F

K

L

L

K

K

H

T

K

K

V

V

T

V

A

G

V

V

E

D

K

T

K

S

G

G

K

D

E

G

V

V

T

K

V

L

K

T

A

V

D

E

-

P

N

S

A

A

K

G

G

G

E

E

T

Q

V

T

E

I

I

I

K

E

G

A

D

D

Y

V

V

V

L

L

V

V

S

S

I

A

G

G

R

R

R

T

P

P

Y

F

T

T

D

S

G

G

L

L

N

N

A

L

E

D

A

K

A

I

G

G

L

V

K

E

L

T

T

D

D

K

R

L

G

G

Q

R

I

I

E

L

V

V

N

N

D

E

H

R

L

F

Q

S

T

T

N

N

V

V

P

S

N

G

I

V

Y

Y

A

A

I

I

G

G

D
|
D

V

V

V

I

K

P

G

G

A

P

M
|
M

L
|
L

A
|
A

H
|
H

K

K

A

A

E

E

E

E

E

D

G

G

V

V

F

A

V

C

A

V

E

E

T

Y

L

L

V

A

G

G

Q

K

K

V

P

G

H

H

I

V

N

D

Y

Y

N

D

L

K

I

V

P

P

G

G

V

V

V

V

Y

Y

T

T

W

N

P

P

E

E

V

V

A

A

A

S

V

V

G

G

Y

K

T

T

E

E

E

E

Q

Q

L

V

K

K

E

E

K

T

G

G

I

V

K

E

Y

Y

K

R

A

V

G

G

K

K

F

F

P

P

F

F

M

M

A

A

S

N

G

S

R

R

A

A

R

K

A

A

S

I

M

D

D

N

T

A

D

E

G

G

L

L

V

V

K

K

V

I

L

I

A

A

D

E

A

K

E

E

T

T

D

D

E

K

I

I

L

L

G

G

V

V

H

H

M

I

I

M

G

A

P

P

R

N

T

A

A

G

D

E

M

L

I

I

A

H

E

E

A

A

V

A

V

I

A

A

M

L

E

Q

Y

Y

R

D

A

A

S

S

A

S

E

E

D

D

I

I

A

A

R

R

M

V

S

C

H

H

A

A

H

H

P

P

T

T

Y

M

T

S

E

E

A

A

F

I

K

K

E

E

A

A

C

A

L

M

A

A

A

T

T

Y

D

D

N

-

R

K

A

P

L

I

H

H

I

I

67:76 (vs. 32:41, 70% identical) binding FAD
C76 (= C41) modified: Disulfide link with 81, Redox-active
C81 (= C46) modified: Disulfide link with 76, Redox-active
G149 (= G114) binding FAD
D348 (= D312) binding FAD
MLAH 354:357 (= MLAH 318:321) binding FAD

Sites not aligning to the query:

1:31 modified: transit peptide, Mitochondrion

2yquB Crystal structures and evolutionary relationship of two different lipoamide dehydrogenase(e3s) from thermus thermophilus
52% identity, 100% coverage: 3:466/466 of query aligns to 2:455/455 of 2yquB

query
sites
2yquB

Y

Y

D

D

V

L

I

L

V

V

I

I

G
|
G

S

A

G
|
G

P
|
P

G
|
G

G

G

Y

Y

V

V

A

A

A

A

I

I

R

R

C

A

A

A

Q

Q

L

L

G

G

M

M

K

K

T

V

A

G

I

V

V

V

E
|
E

K
|
K

Y

E

P

K

T

A

L
|
L

G

G

G
|
G

T
|
T

C
|
C

L

L

N
x
R

V

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

A

A

L

L

L

L

D

E

S

T

S

T

E

E

H

R

Y

I

H

Y

N

E

A

A

A

K

H

K

T

G

F

L

K

L

T

-

H

-

G

G

I

A

N

K

L

V

S

K

N
x
G

L
x
V

K

E

V

L

D

D

L

L

K

P

Q

A

M

L

I

M

T

A

R

H

K

K

N

D

D

K

V

V

V

V

K

Q

Q

A

N

N

T

T

D

Q

G

G

I

V

Q

E

Y

F

L

L

M

F

K

K

K

K

N

N

K

G

I

I

E

A

V

R

H

H

Q

Q

G

G

V
x
T

G
x
A

S

R

F

F

V

L

D

S

K

E

T

R

T

K

V

V

K

L

V

V

T

E

K

E

D

-

D

-

G

-

S

T

S

G

S

E

D

E

I

L

Q

E

G

A

K

R

N

Y

I

I

I

L

V

I

A

A

T
|
T

G
|
G

S

S

K

A

P

P

S

L

N

I

L

P

P

P

F

W

I

A

K

Q

L

V

D

D

K

Y

D

E

R

R

V

V

I

V

T

T

S

S

T

T

E

E

A

A

L

L

N

S

L

F

K

P

E

E

T

V

P

P

K

K

H

R

L

L

V

I

V

V

I

V

G

G

G

G

G

G

V
|
V

I
|
I

G

G

L

L

E
|
E

L

L

G

G

S

V

V

V

Y

W

A

H

R

R

L

L

G

G

A

A

K

E

V

V

S

I

V

V

I

L

E

E

F

Y

M

M

D

D

S

R

L

I

I

L

P

P

T

T

M

M

D

D

R

L

T

E

M

V

G

S

K

R

E

A

L

A

Q

E

K

R

S

V

L

F

K

K

K

K

I

Q

G

G

F

L

E

T

F

I

F

R

L

T

K

G

H

V

K

R

V

V

T

T

A

A

V

V

E

V

K

P

K

E

G

A

K

K

E

G

V

A

T

R

V

V

K

E

A

L

D

E

N

G

A

-

K

-

G

G

E

E

T

V

V

L

E

E

I

-

K

-

G

A

D

D

Y

R

V

V

L

L

V

V

S

A

I

V

G

G

R

R

R

R

P

P

Y
|
Y

T

T

D

E

G

G

L

L

N

S

A

L

E

E

A

N

A

A

G

G

L

L

K

S

L

T

T

D

D

E

R

R

G

G

Q

R

I

I

E

P

V

V

N

D

D

E

H

H

L

L

Q

R

T

T

N

R

V

V

P

P

N

H

I

I

Y

Y

A

A

I

I

G
|
G

D
|
D

V

V

V

V

K

R

G

G

A

P

M
|
M

L
|
L

A
|
A

H
|
H

K

K

A

A

E
x
S

E

E

E

E

G

G

V

I

F

A

V

A

A

V

E

E

T

H

L

M

V

V

G

R

Q

G

K

F

P

G

H

H

I

V

N

D

Y

Y

N

Q

L

A

I

I

P

P

G

S

V

V

V

V

Y

Y

T

T

W

H

P

P

E

E

V

I

A

A

A

A

V

V

G

G

Y

Y

T

T

E

E

E

E

Q

E

L

L

K

K

E

A

K

Q

G

G

I

I

K

P

Y

Y

K

K

A

V

G

G

K

K

F

F

P

P

F

Y

M

S

A

A

S

S

G

G

R

R

A

A

R

R

A

A

S

M

M

G

D

E

T

T

D

E

G

G

L

F

V

I

K

K

V

V

L

L

A

A

D

H

A

A

E

K

T

T

D

D

E

R

I

I

L

L

G

G

V

V

H

H

M

G

I

I

G

G

P

A

R

R

T

V

A

G

D

D

M

V

I

L

A

A

E

E

A

A

V

A

V

L

A

A

M

L

E

F

Y

F

R

K

A

A

S
|
S

A

A

E

E

D
|
D

I

L

A

G

R

R

M

A

S

P

H
|
H

A

A

H
|
H

P

P

T

S

Y

L

T

S

E
|
E

A

I

F

L

K

K

E

E

A

A

C

A

L

L

A

A

A

A

T

W

D

E

N

-

R

R

A

P

L

I

H

H

I

L

active site: P11 (= P12), L36 (= L37), C40 (= C41), C45 (= C46), S48 (= S49), G72 (≠ N75), V73 (≠ L76), V177 (= V183), E181 (= E187), S314 (≠ E324), H432 (= H442), H434 (= H444), E439 (= E449)
binding carbonate ion: A310 (= A320), S314 (≠ E324), S423 (= S433), D426 (= D436)
binding flavin-adenine dinucleotide: G8 (= G9), G10 (= G11), P11 (= P12), G12 (= G13), E31 (= E32), K32 (= K33), G38 (= G39), T39 (= T40), C40 (= C41), R42 (≠ N43), G44 (= G45), C45 (= C46), K49 (= K50), T110 (≠ V113), A111 (≠ G114), T137 (= T143), G138 (= G144), I178 (= I184), Y265 (= Y275), G301 (= G311), D302 (= D312), M308 (= M318), L309 (= L319), A310 (= A320), H311 (= H321)

2yquA Crystal structures and evolutionary relationship of two different lipoamide dehydrogenase(e3s) from thermus thermophilus
52% identity, 100% coverage: 3:466/466 of query aligns to 2:455/455 of 2yquA

query
sites
2yquA

Y

Y

D

D

V

L

I

L

V

V

I

I

G
|
G

S

A

G
|
G

P
|
P

G
|
G

G

G

Y

Y

V

V

A

A

A

A

I

I

R

R

C

A

A

A

Q

Q

L

L

G

G

M

M

K

K

T

V

A

G

I

V

V

V

E
|
E

K
|
K

Y

E

P

K

T

A

L
|
L

G

G

G
|
G

T
|
T

C
|
C

L

L

N
x
R

V

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

A

A

L

L

L

L

D

E

S

T

S

T

E

E

H

R

Y

I

H

Y

N

E

A

A

A

K

H

K

T

G

F

L

K

L

T

-

H

-

G

G

I

A

N

K

L

V

S

K

N
x
G

L
x
V

K

E

V

L

D

D

L

L

K

P

Q

A

M

L

I

M

T

A

R

H

K

K

N

D

D

K

V

V

V

V

K

Q

Q

A

N

N

T

T

D

Q

G

G

I

V

Q

E

Y

F

L

L

M

F

K

K

K

K

N

N

K

G

I

I

E

A

V

R

H

H

Q

Q

G

G

V
x
T

G
x
A

S

R

F

F

V

L

D

S

K

E

T

R

T

K

V

V

K

L

V

V

T

E

K

E

D

-

D

-

G

-

S

T

S

G

S

E

D

E

I

L

Q

E

G

A

K

R

N

Y

I

I

I

L

V

I

A

A

T
|
T

G
|
G

S

S

K

A

P

P

S

L

N

I

L

P

P

P

F

W

I

A

K

Q

L

V

D

D

K

Y

D

E

R

R

V

V

I

V

T

T

S
|
S

T

T

E

E

A

A

L

L

N

S

L

F

K

P

E

E

T

V

P

P

K

K

H

R

L

L

V

I

V

V

I

V

G

G

G

G

G

G

V
|
V

I
|
I

G

G

L

L

E
|
E

L

L

G

G

S

V

V

V

Y

W

A

H

R

R

L

L

G

G

A

A

K

E

V

V

S

I

V

V

I

L

E

E

F

Y

M

M

D

D

S

R

L

I

I

L

P

P

T

T

M

M

D

D

R

L

T

E

M

V

G

S

K

R

E

A

L

A

Q

E

K

R

S

V

L

F

K

K

K

K

I

Q

G

G

F

L

E

T

F

I

F

R

L

T

K

G

H

V

K

R

V

V

T

T

A

A

V

V

E

V

K

P

K

E

G

A

K

K

E

G

V

A

T

R

V

V

K

E

A

L

D

E

N

G

A

-

K

-

G

G

E

E

T

V

V

L

E

E

I

-

K

-

G

A

D

D

Y

R

V

V

L

L

V

V

S

A

I

V

G

G

R

R

R

R

P

P

Y
|
Y

T

T

D

E

G

G

L

L

N

S

A

L

E

E

A

N

A

A

G

G

L

L

K

S

L

T

T

D

D

E

R

R

G

G

Q

R

I

I

E

P

V

V

N

D

D

E

H

H

L

L

Q

R

T

T

N

R

V

V

P

P

N

H

I

I

Y

Y

A

A

I

I

G
|
G

D
|
D

V

V

V

V

K

R

G

G

A

P

M
|
M

L
|
L

A
|
A

H

H

K

K

A

A

E
x
S

E

E

E

E

G

G

V

I

F

A

V

A

A

V

E

E

T

H

L

M

V

V

G

R

Q

G

K

F

P

G

H

H

I

V

N

D

Y

Y

N

Q

L

A

I

I

P

P

G

S

V

V

V

V

Y

Y

T

T

W

H

P

P

E

E

V

I

A

A

A

A

V

V

G

G

Y

Y

T

T

E

E

E

E

Q

E

L

L

K

K

E

A

K

Q

G

G

I

I

K

P

Y

Y

K

K

A

V

G

G

K

K

F

F

P

P

F

Y

M

S

A

A

S

S

G

G

R

R

A

A

R

R

A

A

S

M

M

G

D

E

T

T

D

E

G

G

L

F

V

I

K

K

V

V

L

L

A

A

D

H

A

A

E

K

T

T

D

D

E

R

I

I

L

L

G

G

V

V

H

H

M

G

I

I

G

G

P

A

R

R

T

V

A

G

D

D

M

V

I

L

A

A

E

E

A

A

V

A

V

L

A

A

M

L

E

F

Y

F

R

K

A

A

S

S

A

A

E

E

D

D

I

L

A

G

R

R

M

A

S

P

H
|
H

A

A

H
|
H

P

P

T

S

Y

L

T

S

E
|
E

A

I

F

L

K

K

E

E

A

A

C

A

L

L

A

A

A

A

T

W

D

E

N

-

R

R

A

P

L

I

H

H

I

L

active site: P11 (= P12), L36 (= L37), C40 (= C41), C45 (= C46), S48 (= S49), G72 (≠ N75), V73 (≠ L76), V177 (= V183), E181 (= E187), S314 (≠ E324), H432 (= H442), H434 (= H444), E439 (= E449)
binding flavin-adenine dinucleotide: G8 (= G9), G10 (= G11), P11 (= P12), G12 (= G13), E31 (= E32), K32 (= K33), G38 (= G39), T39 (= T40), C40 (= C41), R42 (≠ N43), G44 (= G45), C45 (= C46), K49 (= K50), T110 (≠ V113), A111 (≠ G114), T137 (= T143), G138 (= G144), S157 (= S163), I178 (= I184), Y265 (= Y275), G301 (= G311), D302 (= D312), M308 (= M318), L309 (= L319), A310 (= A320)

1dxlA Dihydrolipoamide dehydrogenase of glycine decarboxylase from pisum sativum (see paper)
52% identity, 99% coverage: 4:466/466 of query aligns to 5:467/467 of 1dxlA

query
sites
1dxlA

D

D

V

V

I

V

V

I

I
|
I

G

G

S

G

G

G

P
|
P

G
|
G

G

G

Y

Y

V

V

A

A

A

A

I

I

R

K

C

A

A

A

Q

Q

L

L

G

G

M

F

K

K

T

T

A

T

I

C

V

I

E
|
E

K
|
K

Y
x
R

P

G

T

A

L
|
L

G

G

G
|
G

T
|
T

C
|
C

L

L

N

N

V

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

A

A

L

L

L

L

D

H

S

S

S

S

E

H

H

M

Y

Y

H

H

N

E

A

A

A

K

H

H

T

S

F

F

K

A

T

N

H

H

G

G

I

V

N

K

L

V

S

S

N

N

L

V

K

E

V

I

D

D

L

L

K

A

Q

A

M

M

I

M

T

G

R

Q

K

K

N

D

D

K

V

A

V

V

K

S

Q

N

N

L

T

T

D

R

G

G

I

I

Q

E

Y

G

L

L

M

F

K

K

K

K

N

N

K

K

I

V

E

T

V

Y

H

V

Q

K

G

G

V
x
Y

G
|
G

S

K

F

F

V

V

D

S

K

P

T

S

T

E

V

I

K

S

V

V

T

D

K

T

D

I

D

E

G

G

S

E

S

N

S

T

D

V

I

V

Q

K

G

G

K

K

N

H

I

I

I

I

V

I

A

A

T
|
T

G
|
G

S

S

K

D

P

V

S

K

N

S

L

L

P

P

F

G

I

V

K

T

L

I

D

D

K

E

D

K

R

K

V

I

I

V

T

S

S

S

T

T

E

G

A

A

L

L

N

A

L

L

K

S

E

E

T

I

P

P

K

K

H

K

L

L

V

V

V

V

I

I

G

G

G

A

G

G

V
x
Y

I
|
I

G

G

L

L

E
|
E

L

M

G

G

S

S

V

V

Y

W

A

G

R

R

L

I

G

G

A

S

K

E

V

V

S

T

V

V

I

V

E

E

F

F

M

A

D

S

S

E

L

I

I

V

P

P

T

T

M

M

D

D

R

A

T

E

M

I

G

R

K

K

E

Q

L

F

Q

Q

K

R

S

S

L

L

K

E

K

K

I

Q

G

G

F

M

E

K

F

F

F

K

L

L

K

K

H

T

K

K

V

V

T

V

A

G

V

V

E

D

K

T

K

S

G

G

K

D

E

G

V

V

T

K

V

L

K

T

A

V

D

E

-

P

N

S

A

A

K

G

G

G

E

E

T

Q

V

T

E

I

I

I

K

E

G

A

D

D

Y

V

V

V

L

L

V

V

S

S

I

A

G

G

R
|
R

R

T

P

P

Y

F

T

T

D

S

G

G

L

L

N

N

A

L

E

D

A

K

A

I

G

G

L

V

K

E

L

T

T

D

D

K

R

L

G

G

Q

R

I

I

E

L

V

V

N

N

D

E

H

R

L

F

Q

S

T

T

N

N

V

V

P

S

N

G

I

V

Y

Y

A

A

I

I

G

G

D
|
D

V

V

V

I

K

P

G

G

A

P

M
|
M

L
|
L

A
|
A

H
|
H

K

K

A

A

E

E

E

E

E

D

G

G

V

V

F

A

V

C

A

V

E

E

T

Y

L

L

V

A

G

G

Q

K

K

V

P

G

H

H

I

V

N

D

Y

Y

N

D

L

K

I

V

P

P

G

G

V

V

V

V

Y

Y

T

T

W

N

P

P

E

E

V

V

A

A

A

S

V

V

G

G

Y

K

T

T

E

E

E

E

Q

Q

L

V

K

K

E

E

K

T

G

G

I

V

K

E

Y

Y

K

R

A

V

G

G

K

K

F

F

P

P

F

F

M

M

A

A

S

N

G

S

R

R

A

A

R

K

A

A

S

I

M

D

D

N

T

A

D

E

G

G

L

L

V

V

K

K

V

I

L

I

A

A

D

E

A

K

E

E

T

T

D

D

E

K

I

I

L

L

G

G

V

V

H

H

M

I

I

M

G

A

P

P

R

N

T

A

A

G

D

E

M

L

I

I

A

H

E

E

A

A

V

A

V

I

A

A

M

L

E

Q

Y

Y

R

D

A

A

S

S

A

S

E

E

D

D

I

I

A

A

R

R

M

V

S

C

H
|
H

A

A

H
|
H

P

P

T

T

Y

M

T

S

E
|
E

A

A

F

I

K

K

E

E

A

A

C

A

L

M

A

A

A

T

T

Y

D

D

N

-

R

K

A

P

L

I

H

H

I

I

active site: L38 (= L37), C42 (= C41), C47 (= C46), S50 (= S49), Y184 (≠ V183), E188 (= E187), H444 (= H442), H446 (= H444), E451 (= E449)
binding flavin-adenine dinucleotide: I9 (= I8), P13 (= P12), G14 (= G13), E33 (= E32), K34 (= K33), R35 (≠ Y34), G40 (= G39), T41 (= T40), C42 (= C41), G46 (= G45), C47 (= C46), K51 (= K50), Y114 (≠ V113), G115 (= G114), T144 (= T143), G145 (= G144), Y184 (≠ V183), I185 (= I184), R274 (= R272), D314 (= D312), M320 (= M318), L321 (= L319), A322 (= A320), H323 (= H321)

2eq7A Crystal structure of lipoamide dehydrogenase from thermus thermophilus hb8 with psbdo
52% identity, 98% coverage: 3:461/466 of query aligns to 2:451/452 of 2eq7A

query
sites
2eq7A

Y

Y

D

D

V

L

I

L

V

V

I

I

G

G

S

A

G
|
G

P
|
P

G
|
G

G

G

Y

Y

V

V

A<