SitesBLAST

1a56A Primary sequence and solution conformation of ferricytochromE C-552 from nitrosomonas europaea, nmr, mean structure refined with explicit hydrogen bond constraints (see paper)
35% identity, 62% coverage: 47:124/125 of query aligns to 4:81/81 of 1a56A

query
sites
1a56A

L

L

I

A

S

K

Y

K

S

N

D
x
N

C
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C

S

I

E

A

C
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C

H
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H

K

Q

D

V

D

E

M

T

R

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K
x
V

G
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G

P
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P

A

A

F

L

R

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D

D

I

I

A

A

K

A

R

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Y

Y

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D

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D

Y

A

L

A

D

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L

Y

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L

A

A

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K

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x
I

I

K

S

G

G

G

G
x
S

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S

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G

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W
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W

G

G

N
x
Q

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x
I

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M

S

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P

P

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x
N

P
x
V

E
x
N

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S

E

D

D

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D

D

A

A

K

K

A

A

M

L

V

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I

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L

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binding heme c: N9 (≠ D52), C10 (= C53), C13 (= C56), H14 (= H57), V21 (≠ K64), G22 (= G65), P23 (= P66), K45 (= K88), I46 (≠ V89), S50 (≠ G93), W54 (= W97), Q56 (≠ N99), I57 (≠ P100), M59 (= M102), N62 (≠ H105), V63 (≠ P106), N64 (≠ E107)

351cA Structure of cytochrome c551 from p. Aeruginosa refined at 1.6 angstroms resolution and comparison of the two redox forms (see paper)
32% identity, 62% coverage: 45:122/125 of query aligns to 4:80/82 of 351cA

query
sites
351cA

E

E

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V

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L

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N

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C

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C

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H

K

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D

D

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G

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P

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A

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D

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L
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L

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R

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G

G

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W

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G

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-

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binding protoporphyrin ix containing fe: C12 (= C53), C15 (= C56), H16 (= H57), G24 (= G65), P25 (= P66), Y27 (≠ F68), L44 (= L85), R47 (≠ K88), I48 (≠ V89), Q53 (≠ T94), G54 (= G95), V55 (≠ S96), W56 (= W97), G57 (= G98), I59 (= I101), P60 (≠ M102), M61 (≠ S103)

P00099 Cytochrome c-551; Cytochrome C8; Cytochrome c551 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 4 papers)
32% identity, 62% coverage: 45:122/125 of query aligns to 26:102/104 of P00099

query
sites
P00099

E
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E

V
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V

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L

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F

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Y
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N

S
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K

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C

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A

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C

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H

K
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A

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D

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D

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G

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G
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x
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W
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W

G
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G

N

-

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M
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P

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S

H38 (= H57) binding axial binding residue
M83 (≠ S103) binding axial binding residue

Sites not aligning to the query:

1:22 signal peptide
23:104 modified: mature protein, Cytochrome c-551

5aurA Hydrogenobacter thermophilus cytochrome c552 dimer formed by domain swapping at n-terminal region (see paper)
34% identity, 64% coverage: 45:124/125 of query aligns to 2:83/83 of 5aurA

query
sites
5aurA

E

E

V

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C

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H

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-

D

D

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G

G

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-

G

-

K

-

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-

G

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P

A

A

F
x
Y

R

A

D

D

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A

A

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Y
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D

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Y

L

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K

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G

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x
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G

S
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V

W
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W

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G

N

S

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x
V

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M

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E

N

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binding heme c: C10 (= C53), C13 (= C56), H14 (= H57), P26 (= P66), Y28 (≠ F68), Y35 (= Y75), Y44 (≠ L84), I49 (≠ V89), S54 (≠ T94), G55 (= G95), V56 (≠ S96), W57 (= W97), G58 (= G98), V60 (≠ P100), P61 (≠ I101), M62 (= M102), Q65 (≠ H105)

2exvA Crystal structure of the f7a mutant of the cytochrome c551 from pseudomonas aeruginosa (see paper)
32% identity, 62% coverage: 45:122/125 of query aligns to 4:80/82 of 2exvA

query
sites
2exvA

E

E

V

V

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L

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C

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W

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E

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A

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S

binding heme c: C12 (= C53), C15 (= C56), H16 (= H57), G24 (= G65), P25 (= P66), R47 (≠ K88), I48 (≠ V89), S52 (≠ G93), Q53 (≠ T94), G54 (= G95), V55 (≠ S96), W56 (= W97), G57 (= G98), I59 (= I101), P60 (≠ M102), M61 (≠ S103), N64 (≠ P106)

5xecC Heterodimer constructed from pa cyt c551-ht cyt c552 and ht cyt c552- pa cyt c551 chimeric proteins (see paper)
29% identity, 62% coverage: 45:122/125 of query aligns to 2:78/80 of 5xecC

query
sites
5xecC

E

E

V

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L

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C

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C

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D

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G

G

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W
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W

G
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G

N

-

P

P

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I

M

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M

P

P

H

P

P
x
N

E

A

I

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N

S

E

D

D

D

D

E

A

A

K

Q

A

T

M

L

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S

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Y

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L

S

S

binding heme c: C10 (= C53), C13 (= C56), H14 (= H57), P23 (= P66), Y25 (≠ F68), L42 (= L85), R45 (≠ K88), Q51 (≠ T94), V53 (≠ S96), W54 (= W97), G55 (= G98), I57 (= I101), M59 (≠ S103), N62 (≠ P106)

Query Sequence

>WP_008199323.1 NCBI__GCF_000166275.1:WP_008199323.1
MILSACTQKPSADTKDTSLIKEKDYIRVIEGIDEPLDHEMIQRGEVLISYSDCSECHKDD
MRSKGPAFRDIAKRYPTSQVYLDLLARKVISGGTGSWGNPIMSPHPEINEDDAKAMVSYI
LSLED

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory