SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

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Find homologs in fast.genomics

Fitness BLAST: loading...

Comparing WP_008202270.1 NCBI__GCF_000166275.1:WP_008202270.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree

Found 20 (the maximum) hits to proteins with known functional sites (download)

4ilkA Crystal structure of short chain alcohol dehydrogenase (rspb) from e. Coli cft073 (efi target efi-506413) complexed with cofactor nadh
30% identity, 99% coverage: 1:337/340 of query aligns to 4:337/337 of 4ilkA

query
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4ilkA

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active site: C40 (= C37), G41 (= G38), S42 (≠ G39), H45 (≠ N42), H59 (= H59), E60 (= E60), C89 (= C90), C92 (= C93), C95 (= C96), C103 (= C104), A107 (≠ K108), P145 (= P145), A149 (≠ G149), K332 (= K332)
binding manganese (ii) ion: C40 (= C37), H59 (= H59), E60 (= E60), E144 (= E144)
binding 1,4-dihydronicotinamide adenine dinucleotide: G168 (= G168), G170 (= G170), P171 (≠ I171), I172 (≠ V172), D193 (= D191), R194 (≠ L192), R198 (≠ K196), N213 (≠ P211), A235 (= A235), A236 (≠ V236), C237 (≠ G237), I241 (≠ T241), M258 (≠ I258), F260 (≠ Y260)
binding zinc ion: C89 (= C90), C92 (= C93), C95 (= C96), C103 (= C104)

7y9pA Xylitol dehydrogenase s96c/s99c/y102c mutant(thermostabilized form) from pichia stipitis (see paper)
34% identity, 87% coverage: 3:298/340 of query aligns to 5:310/357 of 7y9pA

query
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binding zinc ion: C40 (= C37), H65 (= H59), E66 (= E60), C95 (= C90), C98 (= C93), C101 (= C96), C109 (= C104)

5kiaA Crystal structure of l-threonine 3-dehydrogenase from burkholderia thailandensis
29% identity, 95% coverage: 1:323/340 of query aligns to 2:338/339 of 5kiaA

query
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active site: C37 (= C37), G38 (= G38), T39 (≠ G39), H42 (≠ N42), H61 (= H59), E62 (= E60), C91 (= C90), C94 (= C93), C97 (= C96), C105 (= C104), V109 (≠ K108), P147 (= P145), A151 (≠ G149), K333 (≠ Q318)
binding calcium ion: D146 (≠ E144), N150 (≠ V148), E288 (≠ N284)
binding zinc ion: C91 (= C90), C94 (= C93), C97 (= C96), C105 (= C104)

2dfvA Hyperthermophilic threonine dehydrogenase from pyrococcus horikoshii (see paper)
32% identity, 91% coverage: 1:308/340 of query aligns to 3:317/346 of 2dfvA

query
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2dfvA

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G

G

M

L

G

L

A

G

I

I

A

A

S

V

S

A

V

K

E

A

R

S

G

G

A

A

-

Y

K

P

V

V

I

I

A

V

I

S

D
x
E

L
x
P

D

S

D

D

N

F

K
x
R

L

R

E

E

I

L

A

A

K

K

I

V

G

G

V

A

A

D

H

Y

T

V

I

I

N

N

P

P

S

F

K

E

V

E

D

D

L

V

H

V

E

K

T

E

L

V

Q

M

T

D

I

I

T

T

N

D

Q

G

D

N

G

G

P

V

D

D

V

V

I

F

I

L

E

E

A
x
F

V
x
S

G

G

S
x
A

P

P

T

K

T

A

Y

L

R

E

A

Q

A

G

V

L

E

Q

E

A

V

V

A

T

F

P

T

A

G

G

R

R

V

V

V

S

C

L

I
x
L

G
|
G

Y
x
L

-

Y

-

P

A

G

K

K

K

V

D

T

V

I

D

D

F

F

N

N

T

-

S

N

L

L

F

I

V

I

Q

F

K

K

E

A

I

L

E

T

I

I

L

Y

G

G

-
x
I

-
x
T

S

G

R

R

N

H

C

L

L

W

G

E

D

T

F

W

P

Y

I

T

V

V

I

S

Q

R

Y

L

L

L

E

Q

S

S

G

G

R

K

F

L

P

N

V

L

D

D

E

P

V

I

S

T

active site: C40 (= C37), G41 (= G38), T42 (≠ G39), H45 (≠ N42), H65 (= H59), E66 (= E60), C95 (= C90), C98 (= C93), C101 (= C96), C109 (= C104), K113 (= K108), P151 (= P145), A155 (≠ G149)
binding nicotinamide-adenine-dinucleotide: G175 (= G170), P176 (≠ I171), L177 (≠ V172), E197 (≠ D191), P198 (≠ L192), R202 (≠ K196), F241 (≠ A235), S242 (≠ V236), A244 (≠ S238), L264 (≠ I258), G265 (= G259), L266 (≠ Y260), I289 (vs. gap), T290 (vs. gap)
binding zinc ion: C95 (= C90), C101 (= C96), C109 (= C104)

Sites not aligning to the query:

active site: 340

O58389 L-threonine 3-dehydrogenase; L-ThrDH; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 2 papers)
32% identity, 91% coverage: 1:308/340 of query aligns to 5:319/348 of O58389

query
sites
O58389

M

M

K

V

A

A

L

I

V

M

I

K

T

T

E

K

V

P

G

G

K

Y

-

G

T

A

E

E

I

L

Q

V

E

E

V

V

E

D

K

V

P

P

N

K

I

P

S

G

S

P

E

G

E

E

V

V

L

L

V

I

K

K

V

V

G

L

M

A

V

T

G

S

F

I

C
|
C

G

G

G
x
T

D

D

L

L

N

H

S

-

F

-

R

-

G

-

L

I

F

Y

P

E

L

W

Q

N

E

E

Y

W

-

A

-

Q

-

S

-

R

-

I

-

K

-

P

P

P

N

Q

I

I

I

M

G

G

H
|
H

E
|
E

V

V

G

A

G

G

T

E

I

V

E

V

A

E

I

I

G

G

E

P

N

G

V

V

P

-

D

E

S

G

L

I

S

E

I

V

G

G

T

D

K

Y

V

V

T

S

V

V

Y

E

P

T

Y

H

Q

I

N

V

C
|
C

G

G

T

K

C
|
C

V

Y

A

A

C
|
C

R

R

K

R

G

G

R

Q

P

Y

N

H

C

V

C
|
C

K

Q

T

N

N

T

K

K

T

I

M

F

G

G

V

V

R

D

R

T

P

D

G

G

A

V

M

F

T

A

R

E

Y

Y

I

A

A

V

V

V

P

P

Y

A

Q

Q

D

N

V

I

F

W

P

K

S

N

E

P

K

K

L

S

S

I

L

P

K

P

E

E

L

Y

A

A

-

T

L

L

A

Q

E
|
E

P

P

L

L

T

G

V

N

G

A

F

V

H

D

A

T

A

V

E

L

R

A

G

G

R

P

V

I

N

S

E

G

E

K

D

S

C

-

V

V

A

L

V

I

L

T

G

G

C

A

G

G

I

P

V
x
L

G

G

M

L

G

L

A

G

I

I

A

A

S

V

S

A

V

K

E

A

R

S

G

G

A

A

-

Y

K

P

V

V

I

I

A

V

I

S

D
x
E

L

P

D

S

D

D

N

F

K
x
R

L

R

E

E

I

L

A

A

K

K

I

V

G

G

V

A

A

D

H

Y

T

V

I

I

N

N

P

P

S

F

K

E

V

E

D

D

L

V

H

V

E

K

T

E

L

V

Q

M

T

D

I

I

T

T

N

D

Q

G

D

N

G

G

P

V

D

D

V

V

I

F

I

L

E

E

A

F

V

S

G

G

S

A

P

P

T

K

T

A

Y

L

R

E

A

Q

A

G

V

L

E

Q

E

A

V

V

A

T

F

P

T

A

G

G

R

R

V

V

V

S

C

L

I
x
L

G
|
G

Y
x
L

-

Y

-

P

A

G

K

K

K

V

D

T

V

I

D

D

F

F

N

N

T

-

S

N

L

L

F

I

V

I

Q

F

K

K

E

A

I

L

E

T

I

I

L

Y

G

G

-
x
I

-
x
T

S

G

R
|
R

N

H

C

L

L

W

G

E

D

T

F

W

P

Y

I

T

V

V

I

S

Q

R

Y

L

L

L

E

Q

S

S

G

G

R

K

F

L

P

N

V

L

D

D

E

P

V

I

S

T

C42 (= C37) binding Zn(2+)
T44 (≠ G39) mutation to A: Total loss of enzymatic activity.
H67 (= H59) binding Zn(2+)
E68 (= E60) binding Zn(2+)
C97 (= C90) binding Zn(2+)
C100 (= C93) binding Zn(2+)
C103 (= C96) binding Zn(2+)
C111 (= C104) binding Zn(2+)
E152 (= E144) mutation E->A,Q: Almost complete loss of enzymatic activity.; mutation to C: 120-fold decrease in catalytic efficiency.; mutation to D: Shows 3-fold higher turnover rate and reduced affinities toward L-threonine and NAD(+), compared to wild-type.; mutation to K: Total loss of enzymatic activity.
L179 (≠ V172) binding NAD(+)
E199 (≠ D191) binding NAD(+); mutation to A: Large decrease in affinity for NAD(+).
R204 (≠ K196) binding NAD(+); mutation to A: Large decrease in affinity for NAD(+).
LGL 266:268 (≠ IGY 258:260) binding NAD(+)
IT 291:292 (vs. gap) binding NAD(+)
R294 (= R283) mutation to A: 4000-fold decrease in catalytic efficiency.

4uejA Closed state of galactitol-1-phosphate 5-dehydrogenase from e. Coli in complex with glycerol. (see paper)
33% identity, 83% coverage: 1:282/340 of query aligns to 1:286/346 of 4uejA

query
sites
4uejA

M

M

K

K

A

S

L

V

V

V

I

N

T

D

E

T

V

D

G

G

K

I

T

V

E

R

I

V

Q

A

E

E

V

S

E

V

K

I

P

P

N

E

I

I

S

K

S

H

E

Q

-

D

E

E

V

V

L

R

V

V

K

K

V

I

G

A

M

S

V

S

G

G

F

L

C
|
C

G
|
G

G
x
S

D

D

L

L

N
x
P

S

R

-

I

F

F

R

K

G

N

L

-

F

-

P

G

L

A

Q

H

E

Y

Y

Y

P

P

N

I

I

T

I

L

G

G

H
|
H

E
|
E

V

F

G

S

G

G

T

Y

I

I

E

D

A

A

I

V

G

G

E

S

N

G

V

V

P

-

D

D

S

D

L

L

S

H

I

P

G

G

T

D

K

A

V

V

T

A

V

C

Y

V

P

P

Y

L

Q

L

N

P

C
|
C

G

F

T

T

C
|
C

V

P

A

E

C
|
C

R

L

K

K

G

G

R

F

P

Y

N

S

C

Q

C
|
C

K

A

T

K

N

Y

K
x
D

T

F

M

I

G

G

V

S

R

R

P

D

G

G

A

G

M

F

T

A

R

E

Y

Y

I

I

A

V

V

V

P

K

Y

R

Q

K

D

N

V

V

F

F

-

A

-

L

P

P

S

T

E

D

K

-

L

M

S

P

L

I

K

E

E

D

L

G

A

A

L

F

A

I

E
|
E

P
|
P

L

I

T

T

V

V

G
|
G

F

L

H

H

A

A

A

F

E

H

R

L

G

A

R

Q

V

G

N

C

E

E

E

N

D

K

C

N

V

V

A

I

V

I

L

I

G

G

C

A

G

G

I

T

V

I

G

G

M

L

G

L

A

A

I

I

A

Q

S

C

S

A

V

V

E

A

R

L

G

G

A

A

K

K

-

S

V

V

I

T

A

A

I

I

D

D

L

I

D

S

N

E

K

K

L

L

E

A

I

L

A

A

K

K

K

S

I

F

G

G

V

A

A

M

H

Q

T

T

I

F

N

N

P

S

S

S

K

E

V

M

D

S

L

A

H

P

E

Q

T

M

L

Q

Q

S

T

V

I

L

T

R

N

E

Q

L

D

R

G

F

P

N

D

Q

V

L

I

I

I

L

E

E

A

T

V

A

G

G

S

V

P

P

T

Q

T

T

Y

V

R

E

A

L

A

A

V

V

E

E

E

I

V

A

A

G

F

P

T

H

G

A

R

Q

V

L

V

A

C

L

I

V

G

G

Y

T

A

L

K

H

K

Q

D

D

V

L

D

H

F

L

N

T

T

S

S

A

L

T

F

F

-

G

-

K

-

I

V

L

Q

R

K

K

E

E

I

L

E

T

I

V

L

I

G

G

S

S

active site: C38 (= C37), G39 (= G38), S40 (≠ G39), P43 (≠ N42), H59 (= H59), E60 (= E60), C89 (= C90), C92 (= C93), C95 (= C96), C103 (= C104), D107 (≠ K108), P145 (= P145), G149 (= G149)
binding glycerol: H59 (= H59), E144 (= E144)
binding zinc ion: C89 (= C90), C92 (= C93), C95 (= C96), C103 (= C104)

Sites not aligning to the query:

active site: 341

4a2cA Crystal structure of galactitol-1-phosphate dehydrogenase from escherichia coli (see paper)
33% identity, 83% coverage: 1:282/340 of query aligns to 1:286/346 of 4a2cA

query
sites
4a2cA

M

M

K

K

A

S

L

V

V

V

I

N

T

D

E

T

V

D

G

G

K

I

T

V

E

R

I

V

Q

A

E

E

V

S

E

V

K

I

P

P

N

E

I

I

S

K

S

H

E

Q

-

D

E

E

V

V

L

R

V

V

K

K

V

I

G

A

M

S

V

S

G

G

F

L

C
|
C

G
|
G

G
x
S

D

D

L

L

N
x
P

S

R

-

I

F

F

R

K

G

N

L

-

F

-

P

G

L

A

Q

H

E

Y

Y

Y

P

P

N

I

I

T

I

L

G

G

H
|
H

E
|
E

V

F

G

S

G

G

T

Y

I

I

E

D

A

A

I

V

G

G

E

S

N

G

V

V

P

-

D

D

S

D

L

L

S

H

I

P

G

G

T

D

K

A

V

V

T

A

V

C

Y

V

P

P

Y

L

Q

L

N

P

C
|
C

G

F

T

T

C
|
C

V

P

A

E

C
|
C

R

L

K

K

G

G

R

F

P

Y

N

S

C

Q

C
|
C

K

A

T

K

N

Y

K
x
D

T

F

M

I

G

G

V

S

R

R

P

D

G

G

A

G

M

F

T

A

R

E

Y

Y

I

I

A

V

V

V

P

K

Y

R

Q

K

D

N

V

V

F

F

-

A

-

L

P

P

S

T

E

D

K

-

L

M

S

P

L

I

K

E

E

D

L

G

A

A

L

F

A

I

E

E

P
|
P

L

I

T

T

V

V

G
|
G

F

L

H

H

A

A

A

F

E

H

R

L

G

A

R

Q

V

G

N

C

E

E

E

N

D

K

C

N

V

V

A

I

V

I

L

I

G

G

C

A

G

G

I

T

V

I

G

G

M

L

G

L

A

A

I

I

A

Q

S

C

S

A

V

V

E

A

R

L

G

G

A

A

K

K

-

S

V

V

I

T

A

A

I

I

D

D

L

I

D

S

N

E

K

K

L

L

E

A

I

L

A

A

K

K

K

S

I

F

G

G

V

A

A

M

H

Q

T

T

I

F

N

N

P

S

S

S

K

E

V

M

D

S

L

A

H

P

E

Q

T

M

L

Q

Q

S

T

V

I

L

T

R

N

E

Q

L

D

R

G

F

P

N

D

Q

V

L

I

I

I

L

E

E

A

T

V

A

G

G

S

V

P

P

T

Q

T

T

Y

V

R

E

A

L

A

A

V

V

E

E

E

I

V

A

A

G

F

P

T

H

G

A

R

Q

V

L

V

A

C

L

I

V

G

G

Y

T

A

L

K

H

K

Q

D

D

V

L

D

H

F

L

N

T

T

S

S

A

L

T

F

F

-

G

-

K

-

I

V

L

Q

R

K

K

E

E

I

L

E

T

I

V

L

I

G

G

S

S

active site: C38 (= C37), G39 (= G38), S40 (≠ G39), P43 (≠ N42), H59 (= H59), E60 (= E60), C89 (= C90), C92 (= C93), C95 (= C96), C103 (= C104), D107 (≠ K108), P145 (= P145), G149 (= G149)
binding zinc ion: C89 (= C90), C92 (= C93), C95 (= C96), C103 (= C104)

Sites not aligning to the query:

active site: 341

2eerB Structural study of project id st2577 from sulfolobus tokodaii strain7
28% identity, 94% coverage: 1:320/340 of query aligns to 1:331/347 of 2eerB

query
sites
2eerB

M

M

K

R

A

A

L

M

V

R

I

L

T

V

E

E

V

I

G

G

K

K

-

P

T

L

E

K

I

L

Q

E

E

D

V

I

E

P

K

I

P

P

N

K

I

P

S

K

S

G

E

S

E

Q

V

V

L

L

V

I

K

K

V

I

G

E

M

A

V

A

G

G

F

V

C
|
C

G
x
H

G
x
S

D

D

L

V

N
x
H

S

M

F

R

R

Q

G

G

L

R

F

F

-

G

P

N

L

L

Q

R

-

I

-

V

-

E

-

D

-

L

-

G

-

V

E

K

Y

L

P

P

N

V

I

T

I

L

G

G

H
|
H

E
|
E

V

I

G

A

G

G

T

R

I

I

E

E

A

E

I

V

G

G

E

D

N

E

V

V

P

V

D

-

S

G

L

Y

S

S

I

K

G

G

T

D

K

L

V

V

T

A

V

V

Y

N

P

P

Y

W

Q

E

N

G

C
x
E

G

G

T

N

C
|
C

V

Y

A

Y

C
|
C

R

R

K

I

G

G

R

E

P

E

N

H

C

L

C
|
C

K

D

T

S

N

P

K
x
R

T

W

M

L

G

G

V

I

R

N

R

Y

P

D

G

G

A

A

M

Y

T

A

R

E

Y

Y

I

V

A

L

V

V

P

P

-

H

Y

Y

Q

K

D

Y

V

L

F

Y

P

K

S

L

E

R

K

R

L

L

S

S

L

A

K

V

E

E

L

-

A

-

L

-

A

A

E

A

P

P

L

L

T

T

V
x
C

G

S

-

G

-

V

-
x
T

-

T

F

Y

H

R

A

A

A

V

E

R

R

K

G

A

R

S

V

L

N

D

E

P

E

S

D

K

C

T

V

L

A

V

V

V

L

I

G
|
G

C

A

G

G

I

-

V
x
G

G
|
G

M
x
L

G

G

A

T

I

M

A

A

S

I

S

Q

V

I

E

A

R

K

-

A

-

V

-

S

G

G

A

A

K

T

V

I

I

I

A

G

I

V

D
|
D

L
x
V

D
x
R

D

E

N

E

K

A

L

L

E

E

I

A

A

A

K

K

K

R

I

A

G

G

V

A

A

D

H

Y

T

V

I

I

N

N

P

A

S

S

K

S

V

Q

D

D

L

P

H

V

E

S

T

E

L

I

Q

R

T

R

I

I

T

T

N

Q

Q

G

D

K

G

G

P

A

D

D

V

A

I

V

I

I

E

D

A
x
L

V
x
N

G

N

S

S

P

E

T

K

T

T

Y

L

R

S

A

I

A

Y

V

P

E

Y

E

V

V

L

A

A

F

K

T

Q

G

G

R

K

V

Y

V

V

C

M

I
x
V

G
|
G

Y
x
L

A
x
F

K

G

K

A

D

D

V

L

D

K

F

Y

N

H

T

A

S

P

L

L

F

I

V

T

Q

L

K

N

E

E

I

V

E

Q

I

F

L

I

G

G

S

S

R
x
L

-
x
V

N

G

C

N

L

Q

G

S

D

D

F

F

P

L

I

G

V

I

I

M

Q

S

Y

L

L

A

E

E

S

A

G

G

R

K

F

-

P

-

V

V

D

K

E

P

V

M

V

V

S

T

K

K

I

T

V

M

S

K

I

L

D

E

E

E

A

A

P

N

Q

E

T

A

M

I

active site: C38 (= C37), H39 (≠ G38), S40 (≠ G39), H43 (≠ N42), H68 (= H59), E69 (= E60), E98 (≠ C90), C101 (= C93), C104 (= C96), C112 (= C104), R116 (≠ K108), C154 (≠ V148), T158 (vs. gap)
binding nicotinamide-adenine-dinucleotide: C38 (= C37), H39 (≠ G38), S40 (≠ G39), H43 (≠ N42), C154 (≠ V148), T158 (vs. gap), G178 (= G168), G181 (≠ V172), G182 (= G173), L183 (≠ M174), D203 (= D191), V204 (≠ L192), R205 (≠ D193), L247 (≠ A235), N248 (≠ V236), V270 (≠ I258), G271 (= G259), L272 (≠ Y260), F273 (≠ A261), L295 (≠ R283), V296 (vs. gap)
binding zinc ion: E98 (≠ C90), C101 (= C93), C104 (= C96), C112 (= C104)

Sites not aligning to the query:

active site: 342
binding nicotinamide-adenine-dinucleotide: 342

4cpdA Alcohol dehydrogenase tadh from thermus sp. Atn1
30% identity, 95% coverage: 1:324/340 of query aligns to 1:334/346 of 4cpdA

query
sites
4cpdA

M

M

K

R

A

A

L

V

V

V

I

F

T

E

E

N

V

K

G

E

K

R

T

V

E

A

I

V

Q

K

E

E

V

V

E

N

K

A

P

P

N

R

I

L

S

Q

S

H

E

P

-

L

E

D

V

A

L

L

V

V

K

R

V

V

G

H

M

L

V

A

G

G

F

I

C
|
C

G
|
G

G
x
S

D

D

L

L

N
x
H

S

L

F

Y

R

H

G

G

L

K

F

I

P

P

L

V

Q

L

E

P

Y

G

P

-

N

S

I

V

I

L

G

G

H
|
H

E
|
E

V

F

G

V

G

G

T

Q

I

V

E

E

A

A

I

V

G

G

E

E

N

G

V

I

P

Q

D

D

S

-

L

L

S

Q

I

P

G

G

T

D

K

W

V

V

T

V

V

G

Y

P

P

F

Y

H

Q

I

N

A

C
|
C

G

G

T

T

C
|
C

V

P

A

Y

C
|
C

R

R

K

R

G

H

R

Q

P

Y

N

N

C

L

C
|
C

K

E

T

R

N

G

K
x
G

T

V

M

Y

G

G

V

Y

-

G

-

P

-

M

-

F

-

G

R

N

R

L

P

Q

G

G

A

A

M

Q

T

A

R

E

Y

I

I

L

A

R

V

V

P

P

Y

F

Q

S

D

N

V

V

-

N

-

L

-

R

-

K

F

L

P

P

S

P

E

N

K

L

L

S

S

P

L

E

K

R

E

A

L

I

A

F

L

A

A

G

E
x
D

P

I

L

L

T

S

V
x
T

G

A

F

Y

H

G

A

G

A

L

E

I

R

Q

G

G

R

Q

V

L

N

R

E

P

E

G

D

D

C

S

V

V

A

A

V

V

L

I

G

G

C

A

G
|
G

I
x
P

V
|
V

G

G

M

L

G

M

A

A

I

I

A

E

S

V

S

A

V

Q

E

V

R

L

G

G

A

A

-

S

K

K

V

I

I

L

A

A

I

I

D
|
D

L
x
R

D

I

D

P

N

E

K
x
R

L

L

E

E

I

R

A

A

K

A

K

S

I

L

G

G

V

-

A

A

H

I

T

P

I

I

N

N

P

A

S

E

K

Q

V

E

D

N

L

P

H

V

E

R

T

R

L

V

Q

R

T

S

I

E

T

T

N

N

Q

D

D

E

G

G

P

P

D

D

V

L

I

V

I

L

E

E

A
|
A

V
|
V

G

G

S

G

P

A

T

A

T

T

Y

L

R

S

A

L

A

A

V

L

E

E

E

M

V

V

A

R

F

P

T

G

G

G

R

R

V

V

V

S

C

A

I
x
V

G

G

Y
x
V

A

D

K

N

K

A

-

P

D

S

V

F

D

P

F

F

N

P

T

L

S

A

L

S

F

G

V

L

Q

V

K

K

E

D

I

L

E

T

I

F

-

R

L

I

G

G

S
x
L

R
x
A

N

N

C

V

L

H

G

L

D

Y

F

I

P

D

I

A

V

V

I

L

Q

A

Y

L

L

L

E

A

S

S

G

G

R

R

F

L

P

Q

V

P

D

E

E

R

V

I

V

V

S

S

K

H

I

Y

V

L

S

P

I

L

D

E

E

E

A

A

P

P

Q

R

T

G

M

Y

I

E

D

L

W
x
F

D

D

active site: C38 (= C37), G39 (= G38), S40 (≠ G39), H43 (≠ N42), H59 (= H59), E60 (= E60), C89 (= C90), C92 (= C93), C95 (= C96), C103 (= C104), G107 (≠ K108), D152 (≠ E144), T156 (≠ V148)
binding nicotinamide-adenine-dinucleotide: G39 (= G38), S40 (≠ G39), T156 (≠ V148), G178 (= G170), P179 (≠ I171), V180 (= V172), D200 (= D191), R201 (≠ L192), R205 (≠ K196), A243 (= A235), V244 (= V236), V266 (≠ I258), V268 (≠ Y260), L292 (≠ S282), A293 (≠ R283), F333 (≠ W323)
binding zinc ion: C38 (= C37), H59 (= H59), C89 (= C90), C92 (= C93), C95 (= C96), C103 (= C104), D152 (≠ E144)

Sites not aligning to the query:

active site: 340

4ej6A Crystal structure of a putative zinc-binding dehydrogenase (target psi-012003) from sinorhizobium meliloti 1021
27% identity, 99% coverage: 1:335/340 of query aligns to 4:338/343 of 4ej6A

query
sites
4ej6A

M

M

K

K

A

A

L

V

V

R

I

L

T

E

E

S

V

V

G

G

K

N

T

I

E

S

I

V

Q

R

E

N

V

V

E

G

K

I

P

P

N

E

I

P

S

G

S

P

E

D

V

L

L

L

V

V

K

K

V

V

G

E

M

A

V

C

G

G

F

I

C
|
C

G
|
G

G
x
T

D

D

L

R

N
x
H

S

L

F

L

R

H

G

G

L

E

F

F

P

P

L

-

Q

S

E

T

Y

P

P

P

N

V

I

T

I

L

G

G

H
|
H

E
|
E

V

F

G

C

G

G

T

I

I

V

E

V

A

E

I

A

G

G

E

S

N

A

V

V

P

R

D

D

S

-

L

I

S

A

I

P

G

G

T

A

K

R

V

I

T

T

V

G

Y

D

P

P

Y

N

Q

I

N

S

C
|
C

G

G

T

R

C
|
C

V

P

A

Q

C
|
C

R

Q

K

A

G

G

R

R

P

V

N

N

C

L

C
|
C

K

R

T

N

N

L

K
x
R

T

A

M

I

G

G

V

I

R

H

R

R

P

D

G

G

A

G

M

F

T

A

R

E

Y

Y

I

V

A

L

V

V

P

P

Y

R

Q

K

D

Q

V

A

F

F

P

-

S

E

E

I

K

P

L

L

S

T

L

L

K

D

E

P

L

V

-

H

-

G

A

A

L

F

A

C

E

E

P
|
P

L

L

T

A

V

C

G
x
C

F

L

H

H

A

G

A

V

E

D

R

L

G

S

R

G

V

I

N

K

E

A

E

G

D

S

C

T

V

V

A

A

V

I

L

L

G

G

C

G

G

G

I

V

V

I

G

G

M

L

G

L

A

T

I

V

A

Q

S

L

S

A

V

R

E

L

R

A

G

G

A

A

K

T

V

T

I

V

A

I

I

L

D

S

L

T

D

R

D

Q

-

A

N

T

K

K

L

R

E

R

I

L

A

A

K

E

I

V

G

G

V

A

A

T

H

A

T

T

I

V

N

D

P

P

S

S

K

A

V

G

D

D

L

V

H

V

E

E

T

A

L

I

Q

A

T

G

I

P

T

V

N

G

-

L

-

V

Q

P

D

G

G

G

P

V

D

D

V

V

I

V

I

I

E

E

A

C

V

A

G

G

S

V

P

A

T

E

T

T

Y

V

R

K

A

Q

A

S

V

T

E

R

E

L

V

A

A

K

F

A

T

G

G

G

R

T

V

V

C

I

I

L

G

G

Y

V

A

L

K

P

K

Q

-

G

-

E

D

K

V

V

D

E

F

I

N

E

T

P

S

F

L

D

F

I

V

L

Q

F

K

R

E

E

I

L

E

R

I

V

L

L

G

G

S

S

R

F

N

I

C

N

L

P

G

F

D

V

F

H

P

R

I

R

V

A

I

A

Q

D

Y

L

L

V

E

A

S

T

G

G

R

A

F

I

P

E

V

I

D

D

E

R

V

M

V

I

S

S

K

R

I

R

V

I

S

S

I

L

D

D

E

E

A

A

P

P

Q

-

T

-

M

-

I

-

D

D

W

V

D

I

A

S

N

N

P

P

V

A

G

A

I

A

T

G

K

E

I

V

M
x
K

I

V

active site: C40 (= C37), G41 (= G38), T42 (≠ G39), H45 (≠ N42), H61 (= H59), E62 (= E60), C91 (= C90), C94 (= C93), C97 (= C96), C105 (= C104), R109 (≠ K108), P147 (= P145), C151 (≠ G149), K337 (≠ M334)
binding zinc ion: C91 (= C90), C94 (= C93), C97 (= C96), C105 (= C104)

4ejmA Crystal structure of a putative zinc-binding dehydrogenase (target psi-012003) from sinorhizobium meliloti 1021 bound to NADP
27% identity, 99% coverage: 1:335/340 of query aligns to 4:338/342 of 4ejmA

query
sites
4ejmA

M

M

K

K

A

A

L

V

V

R

I

L

T

E

E

S

V

V

G

G

K

N

T

I

E

S

I

V

Q

R

E

N

V

V

E

G

K

I

P

P

N

E

I

P

S

G

S

P

E

D

V

L

L

L

V

V

K

K

V

V

G

E

M

A

V

C

G

G

F

I

C
|
C

G
|
G

G
x
T

D

D

L

R

N
x
H

S

L

F

L

R

H

G

G

L

E

F

F

P

P

L

-

Q

S

E

T

Y

P

P

P

N

V

I

T

I

L

G

G

H
|
H

E
|
E

V

F

G

C

G

G

T

I

I

V

E

V

A

E

I

A

G

G

E

S

N

A

V

V

P

R

D

D

S

-

L

I

S

A

I

P

G

G

T

A

K

R

V

I

T

T

V

G

Y

D

P

P

Y

N

Q

I

N

S

C
|
C

G

G

T

R

C
|
C

V

P

A

Q

C
|
C

R

Q

K

A

G

G

R

R

P

V

N

N

C

L

C
|
C

K

R

T

N

N

L

K
x
R

T

A

M

I

G

G

V

I

R

H

R

R

P

D

G

G

A

G

M

F

T

A

R

E

Y

Y

I

V

A

L

V

V

P

P

Y

R

Q

K

D

Q

V

A

F

F

P

-

S

E

E

I

K

P

L

L

S

T

L

L

K

D

E

P

L

V

-

H

-

G

A

A

L

F

A

C

E

E

P
|
P

L

L

T

A

V

C

G
x
C

F

L

H

H

A

G

A

V

E

D

R

L

G

S

R

G

V

I

N

K

E

A

E

G

D

S

C

T

V

V

A

A

V

I

L

L

G
|
G

C

G

G
|
G

I
x
V

V
x
I

G

G

M

L

G

L

A

T

I

V

A

Q

S

L

S

A

V

R

E

L

R

A

G

G

A

A

K

T

V

T

I

V

A

I

I

L

D

S

L
x
T

D
x
R

D
x
Q

-

A

N

T

K
|
K

L

R

E

R

I

L

A

A

K

E

I

V

G

G

V

A

A

T

H

A

T

T

I

V

N

D

P

P

S

S

K

A

V

G

D

D

L

V

H

V

E

E

T

A

L

I

Q

A

T

G

I

P

T

V

N

G

-

L

-

V

Q

P

D

G

G

G

P

V

D

D

V

V

I

V

I

I

E

E

A
x
C

V

A

G

G

S

V

P

A

T
x
E

T
|
T

Y

V

R

K

A

Q

A

S

V

T

E

R

E

L

V

A

A

K

F

A

T

G

G

G

R

T

V

V

C

I

I
x
L

G

G

Y
x
V

A

L

K

P

K

Q

-

G

-

E

D

K

V

V

D

E

F

I

N

E

T

P

S

F

L

D

F

I

V

L

Q

F

K

R

E

E

I

L

E

R

I

V

L

L

G

G

S

S

R

F

N
x
I

C

N

L

P

G

F

D

V

F

H

P

R

I

R

V

A

I

A

Q

D

Y

L

L

V

E

A

S

T

G

G

R

A

F

I

P

E

V

I

D

D

E

R

V

M

V

I

S

S

K

R

I

R

V

I

S

S

I

L

D

D

E

E

A

A

P

P

Q

-

T

-

M

-

I

-

D

D

W

V

D

I

A

S

N

N

P

P

V

A

G

A

I

A

T

G

K

E

I

V

M
x
K

I

V

active site: C40 (= C37), G41 (= G38), T42 (≠ G39), H45 (≠ N42), H61 (= H59), E62 (= E60), C91 (= C90), C94 (= C93), C97 (= C96), C105 (= C104), R109 (≠ K108), P147 (= P145), C151 (≠ G149), K337 (≠ M334)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G170 (= G168), G172 (= G170), V173 (≠ I171), I174 (≠ V172), T194 (≠ L192), R195 (≠ D193), Q196 (≠ D194), K199 (= K196), C240 (≠ A235), E245 (≠ T240), T246 (= T241), L263 (≠ I258), V265 (≠ Y260), I291 (≠ N284)
binding zinc ion: C91 (= C90), C94 (= C93), C97 (= C96), C105 (= C104)

5vm2A Crystal structure of eck1772, an oxidoreductase/dehydrogenase of unknown specificity involved in membrane biogenesis from escherichia coli
30% identity, 96% coverage: 10:335/340 of query aligns to 12:343/347 of 5vm2A

query
sites
5vm2A

G

G

K

T

T

M

E

K

I

I

Q

I

E

S

V

A

E

E

K

I

P

P

N

V

I

P

S

K

S

E

E

D

E

E

V

V

L

L

V

I

K

K

V

V

G

E

M

Y

V

V

G

G

F

I

C
|
C

G
|
G

G
x
S

D

D

L

V

N
x
H

S

G

F

F

R

E

-

S

G

G

L

P

F

F

P

I

L

P

Q

P

E

K

Y

D

P

P

N

N

I

Q

-

E

-

I

-

G

I

L

G

G

H
|
H

E
|
E

V

C

G

A

G

G

T

T

I

V

E

V

A

A

I

V

G

G

E

S

N

R

V

V

P

-

D

R

S

K

L

F

S

K

I

P

G

G

T

D

K

R

V

V

T

N

V

I

Y

E

P

P

Y

G

Q

V

N

P

C
|
C

G

G

T

H

C
|
C

V

R

A

Y

C
|
C

R

L

K

E

G

G

R

K

P

Y

N

N

C

I

C
|
C

K

P

T

D

N

V

K
x
D

T

F

M

M

G

A

V

T

R

Q

-

P

-

N

R

Y

P

R

G

G

A

A

M

L

T

T

R

H

Y

Y

I

L

A

C

V

H

P

P

Y

E

Q

S

D

F

V

T

F

Y

P

K

-

L

S

P

E

D

K

N

L

M

S

D

L

T

K

M

E

E

L

G

A

T

L

L

A

V

E
|
E

P
|
P

L

A

T

A

V

V

G
|
G

F

M

H

H

A

A

E

M

R

L

G

A

R

D

V

V

N

K

E

P

E

G

D

K

C

K

V

I

A

I

V

I

L

L

G

G

C

A

G

G

I

C

V

I

G

G

M

L

G

M

A

T

I

L

A

Q

S

A

S

C

V

K

E

C

R

L

G

G

A

A

K

T

V

E

I

I

A

A

I

V

-

V

D

D

L

V

D

L

D

E

N

K

K

R

L

L

E

A

I

M

A

A

K

E

K

Q

I

L

G

G

V

A

A

T

H

V

T

V

I

I

N

N

P

G

S

A

K

K

V

E

D

D

L

T

H

I

E

A

T

R

L

C

Q

Q

T

Q

I

F

T

T

N

E

Q

D

D

M

G

G

P

A

D

D

V

I

I

V

I

F

E

E

A

T

V

A

G

G

S

S

P

A

T

V

T

T

Y

V

R

K

A

Q

A

A

V

P

E

Y

E

L

V

V

A

M

F

R

T

G

G

G

R

K

V

I

V

M

C

I

I

V

G

G

Y

T

A

V

K

P

K

G

D

A

V

S

D

A

F

I

N

N

T

F

S

-

L

L

F

K

V

I

Q

N

K

R

E

E

I

V

E

T

I

I

L

Q

G

T

S

V

R

F

N

R

C

Y

L

A

G

N

D

R

F

Y

P

P

I

V

V

T

I

I

Q

E

Y

A

L

I

E

S

S

S

G

G

R

R

F

F

P

D

V

V

D

K

E

S

V

M

V

V

S

T

K

H

I

I

V

Y

S

D

I

Y

D

R

E

D

A

V

P

Q

Q

Q

T

A

M

F

I

E

D

E

W

S

D

V

A

N

N

N

P

K

V

R

G

D

I

I

T

I

K
|
K

I

G

M

V

I

I

active site: C39 (= C37), G40 (= G38), S41 (≠ G39), H44 (≠ N42), H65 (= H59), E66 (= E60), C95 (= C90), C98 (= C93), C101 (= C96), C109 (= C104), D113 (≠ K108), P153 (= P145), G157 (= G149), K340 (= K332)
binding magnesium ion: H65 (= H59), E66 (= E60), E152 (= E144)
binding zinc ion: C95 (= C90), C98 (= C93), C101 (= C96), C109 (= C104)

P07913 L-threonine 3-dehydrogenase; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Escherichia coli (strain K12) (see paper)
25% identity, 91% coverage: 14:324/340 of query aligns to 15:341/341 of P07913

query
sites
P07913

I

M

Q

T

E

D

V

V

E

P

K

V

P

P

N

E

I

L

S

G

S

H

E

N

E

D

V

L

L

L

V

I

K

K

V

I

G

R

M

K

V

T

G

A

F

I

C
|
C

G

G

G

T

D

D

L

V

N

H

S

-

F

-

R

-

G

-

L

I

F

Y

P

N

L

W

Q

D

E

E

Y

W

-

S

-

Q

-

K

-

T

-

I

-

P

-

V

P

P

N

M

I

V

G

G

H

H

E

E

V

Y

G

V

G

G

T

E

I

V

E

V

A

G

I

I

G

G

E

Q

N

E

V

V

P

-

D

K

S

G

L

F

S

K

I

I

G

G

T

D

K

R

V

V

T

S

V

G

Y

E

P

G

Y

H

Q

I

N

T

C

C

G

G

T

H

C

C

V

R

A

N

C

C

R

R

K

G

G

G

R

R

P

T

N

H

C

L

C

C

K

R

T

N

N

T

K

I

T

G

M

V

G

G

V

V

R

N

R

R

P

P

G

G

A

C

M

F

T

A

R

E

Y

Y

I

L

A

V

V

I

P

P

Y

A

Q

F

D

N

V

A

F

F

P

K

-

I

S

P

E

D

K

N

L

I

S

S

L

D

K

D

E

L

L

A

A

A

L

I

A

F

E

D

P

P

L

F

T

G

V

N

G

A

F

V

H

H

A

T

A

A

E

L

R

S

G

F

R

D

V

L

N

V

E

G

E

E

D

D

C

-

V

V

A

L

V

V

L

S

G

G

C

A

G

G

I

P

V

I

G

G

M

I

G

M

A

A

I

A

A

A

S

V

S

A

V

K

E

H

R

V

G

G

A

A

K

R

-

N

V

V

I

V

A

I

I

T

D

D

L

V

D

N

D

E

N

Y

K

R

L

L

E

E

I

L

A

A

K

R

K

K

I

M

G

G

V

I

A

T

H

R

T

A

I

V

N

N

P

V

S

A

K

K

V

E

D

N

L

L

H

N

E

D

T

V

L

M

Q

A

T

E

I

L

T

G

N

M

Q

T

D

E

G

G

P

F

D

D

V

V

I

G

I

L

E

E

A

M

V

S

G

G

S

A

P

P

T

P

T

A

Y

F

R

R

A

T

A

M

V

L

E

D

E

T

V

M

A

N

F

H

T

G

G

G

R

R

V

I

V

A

C

M

I

L

G

G

Y

I

A

P

K

P

K

S

D

D

V

M

D

S

F

I

N

D

T

W

S

T

L

K

F

V

V

I

Q

F

K

K

E

G

I

L

E

F

I

I

L

K

G

G

-

I

-

Y

S

G

R

R

N

E

C

M

L

F

G

E

D

T

F

W

P

Y

I

K

V

M

I

A

Q

A

Y

L

L

I

E

Q

S

S

G

G

-

L

-

D

-

L

-

S

-

P

-

I

-

T

-

H

R

R

F

F

P

S

V

I

D

D

E

D

V

F

V

Q

S

K

K

G

I

F

V

D

S

A

I

M

-

R

-

S

D

G

E

Q

A

S

P

G

Q

K

T

V

M

I

I

L

D

S

W

W

D

D

C38 (= C37) mutation to D: Shows only 1% of wild-type catalytic activity. This mutant can be stimulated to the wild-type activity level after incubation with Zn(+).; mutation to S: Loss of catalytic activity. This mutant cannot be stimulated to the wild-type activity level after incubation with Zn(+).

3gfbA L-threonine dehydrogenase (tktdh) from the hyperthermophilic archaeon thermococcus kodakaraensis (see paper)
29% identity, 91% coverage: 1:308/340 of query aligns to 3:317/347 of 3gfbA

query
sites
3gfbA

M

M

K

Q

A

A

L

I

V

M

I

K

T

T

E

K

V

P

G

A

K

Y

-

G

T

A

E

E

I

L

Q

V

E

E

V

V

E

D

K

V

P

P

N

K

I

P

S

G

S

P

E

G

E

E

V

V

L

L

V

I

K

K

V

V

G

L

M

A

V

T

G

S

F

I

C
|
C

G
|
G

G
x
T

D

D

L

L

N
x
H

S

-

F

-

R

-

G

-

L

I

F

Y

P

E

L

W

Q

N

E

E

Y

W

-

A

-

Q

-

S

-

R

-

I

-

K

-

P

P

P

N

Q

I

I

I

M

G

G

H
|
H

E
|
E

V

V

G

A

G

G

T

E

I

V

E

V

A

E

I

V

G

G

E

P

N

G

V

V

P

E

D

D

S

-

L

L

S

Q

I

V

G

G

T

D

K

Y

V

I

T

S

V

V

Y

E

P

T

Y

H

Q

I

N

V

C
|
C

G

G

T

K

C
|
C

V

Y

A

A

C
|
C

R

K

K

H

G

N

R

R

P

Y

N

H

C

V

C
|
C

K

Q

T

N

N

T

K
|
K

T

I

M

F

G

G

V

V

R

D

R

M

P

D

G

G

A

V

M

F

T

A

R

H

Y

Y

I

A

A

I

V

V

P

P

Y

A

Q

K

D

N

V

A

F

W

P

K

S

N

E

P

K

K

-

D

L

M

S

P

L

P

K

E

E

Y

L

A

A

A

L

L

A

Q

E

E

P
|
P

L

L

T

G

V

N

G
x
A

F

V

H

D

A

T

A

V

E

L

R

A

G

G

R

P

V

I

N

A

E

G

E

R

D

S

C

T

V

L

A

-

V

I

L

T

G
|
G

C

A

G
|
G

I
x
P

V
x
L

G

G

M

L

G

L

A

G

I

I

A

A

S

V

S

A

V

K

E

A

R

S

G

G

A

A

-

Y

K

P

V

V

I

I

A

V

I
x
S

D
x
E

L
x
P

D

S

D

E

N

F

K
x
R

L

R

E

K

I

L

A

A

K

K

I

V

G

G

V

A

A

D

H

Y

T

V

I

V

N

N

P

P

S

F

K

E

V

E

D

D

L

P

H

V

E

K

T

F

L

V

Q

M

T

D

I

I

T

T

N

D

Q

G

D

A

G

G

P

V

D

E

V

V

I

F

I

L

E

E

A
x
F

V
x
S

G

G

S
x
A

P

P

T

K

T

A

Y

L

R

E

A

Q

A

G

V

L

E

K

E

A

V

V

A

T

F

P

T

G

G

G

R

R

V

V

V

S

C

L

I
x
L

G
|
G

Y
x
L

A

F

K

P

K

R

D

E

V

V

-

T

-

I

D

D

F

F

N

N

T

-

S

N

L

L

F

I

V

I

Q

F

K

K

E

A

I

L

E

E

I

V

L

H

G

G

-
x
I

-
x
T

S

G

R

R

N

H

C

L

L

W

G

E

D

T

F

W

P

Y

I

T

V

V

I

S

Q

S

Y

L

L

I

E

Q

S

S

G

G

R

K

F

L

P

N

V

L

D

D

E

P

V

I

S

T

active site: C40 (= C37), G41 (= G38), T42 (≠ G39), H45 (≠ N42), H65 (= H59), E66 (= E60), C95 (= C90), C98 (= C93), C101 (= C96), C109 (= C104), K113 (= K108), P151 (= P145), A155 (≠ G149)
binding nicotinamide-adenine-dinucleotide: G173 (= G168), G175 (= G170), P176 (≠ I171), L177 (≠ V172), S196 (≠ I190), E197 (≠ D191), P198 (≠ L192), R202 (≠ K196), F241 (≠ A235), S242 (≠ V236), A244 (≠ S238), L264 (≠ I258), G265 (= G259), L266 (≠ Y260), I289 (vs. gap), T290 (vs. gap)

Sites not aligning to the query:

active site: 340

Q5JI69 L-threonine 3-dehydrogenase; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) (see paper)
29% identity, 91% coverage: 1:308/340 of query aligns to 5:319/350 of Q5JI69

query
sites
Q5JI69

M

M

K

Q

A

A

L

I

V

M

I

K

T

T

E

K

V

P

G

A

K

Y

-

G

T

A

E

E

I

L

Q

V

E

E

V

V

E

D

K

V

P

P

N

K

I

P

S

G

S

P

E

G

E

E

V

V

L

L

V

I

K

K

V

V

G

L

M

A

V

T

G

S

F

I

C

C

G

G

G

T

D

D

L

L

N

H

S

-

F

-

R

-

G

-

L

I

F

Y

P

E

L

W

Q

N

E

E

Y

W

-

A

-

Q

-

S

-

R

-

I

-

K

-

P

P

P

N

Q

I

I

I

M

G

G

H

H

E

E

V

V

G

A

G

G

T

E

I

V

E

V

A

E

I

V

G

G

E

P

N

G

V

V

P

E

D

D

S

-

L

L

S

Q

I

V

G

G

T

D

K

Y

V

I

T

S

V

V

Y

E

P

T

Y

H

Q

I

N

V

C

C

G

G

T

K

C

C

V

Y

A

A

C

C

R

K

K

H

G

N

R

R

P

Y

N

H

C

V

C

C

K

Q

T

N

N

T

K

K

T

I

M

F

G

G

V

V

R

D

R

M

P

D

G

G

A

V

M

F

T

A

R

H

Y

Y

I

A

A

I

V

V

P

P

Y

A

Q

K

D

N

V

A

F

W

P

K

S

N

E

P

K

K

-

D

L

M

S

P

L

P

K

E

E

Y

L

A

A

A

L

L

A

Q

E

E

P

P

L

L

T

G

V

N

G

A

F

V

H

D

A

T

A

V

E

L

R

A

G

G

R

P

V

I

N

A

E

G

E

R

D

S

C

T

V

L

A

-

V

I

L

T

G

G

C

A

G

G

I

P

V
x
L

G

G

M

L

G

L

A

G

I

I

A

A

S

V

S

A

V

K

E

A

R

S

G

G

A

A

-

Y

K

P

V

V

I

I

A

V

I

S

D
x
E

L

P

D

S

D

E

N

F

K
x
R

L

R

E

K

I

L

A

A

K

K

I

V

G

G

V

A

A

D

H

Y

T

V

I

V

N

N

P

P

S

F

K

E

V

E

D

D

L

P

H

V

E

K

T

F

L

V

Q

M

T

D

I

I

T

T

N

D

Q

G

D

A

G

G

P

V

D

E

V

V

I

F

I

L

E

E

A

F

V

S

G

G

S

A

P

P

T

K

T

A

Y

L

R

E

A

Q

A

G

V

L

E

K

E

A

V

V

A

T

F

P

T

G

G

G

R

R

V

V

V

S

C

L

I
x
L

G
|
G

Y
x
L

A

F

K

P

K

R

D

E

V

V

-

T

-

I

D

D

F

F

N

N

T

-

S

N

L

L

F

I

V

I

Q

F

K

K

E

A

I

L

E

E

I

V

L

H

G

G

-
x
I

-
x
T

S

G

R

R

N

H

C

L

L

W

G

E

D

T

F

W

P

Y

I

T

V

V

I

S

Q

S

Y

L

L

I

E

Q

S

S

G

G

R

K

F

L

P

N

V

L

D

D

E

P

V

I

S

T

L179 (≠ V172) binding NAD(+)
E199 (≠ D191) binding NAD(+)
R204 (≠ K196) binding NAD(+)
LGL 266:268 (≠ IGY 258:260) binding NAD(+)
IT 291:292 (vs. gap) binding NAD(+)

5ylnA Zinc dependent alcohol dehydrogenase 2 from streptococcus pneumonia - apo form
29% identity, 93% coverage: 1:315/340 of query aligns to 5:321/348 of 5ylnA

query
sites
5ylnA

M

M

K

K

A

A

L

Y

V

T

I

Y

T

V

E

K

V

P

G

G

K

L

T

A

E

S

I

F

Q

V

E

D

V

V

E

D

K

K

P

P

N

V

I

I

S

R

S

K

E

P

E

T

V

A

L

I

V

V

K

R

V

I

G

V

M

K

V

T

G

T

F

I

C
|
C

G

G

G
x
T

D

D

L

L

N
x
H

S

I

F

I

R

K

G

G

L

D

F

V

P

P

L

T

Q

C

E

Q

Y

S

P

G

N

T

I

I

I

L

G

G

H
|
H

E

E

V

G

G

I

G

G

T

I

I

V

E

E

A

E

I

V

G

G

E

E

N

G

V

V

P

S

D

N

S

-

L

F

S

K

I

K

G

G

T

D

K

K

V

V

T

L

V

I

Y

S

P

C

Y

V

Q

C

N

A

C
|
C

G

G

T

K

C
|
C

V

Y

A

Y

C
|
C

R

K

K

K

G

G

R

I

P

Y

N

A

C

H

C
|
C

K

E

T

-

N

D

K

E

T

G

M

G

G

W

V

I

R

F

R

I

P

D

G

G

A

M

M

Q

T

A

R

E

Y

Y

I

L

A

R

V

V

P

P

Y

H

Q

A

D

D

-

N

V

T

F

L

P

Y

S

H

E

T

K

P

L

E

S

D

L

L

K

S

E

D

L

E

A

A

L

L

A

V

E

M

P

L

L

L

T

P

V

T

G

G

F

Y

H

E

-

I

A

G

A

V

E

L

R

K

G

G

R

K

V

V

N

E

E

P

E

G

D

C

C

S

V

V

A

A

V

I

L

I

G

G

C

S

G

G

I

P

V

V

G

G

M

L

G

A

A

A

I

L

A

L

S

T

S

A

-

Q

V

F

E

Y

R

S

G

P

A

A

K

K

V

L

I

I

A

M

I

V

D

D

L

L

D

D

N

N

K

R

L

L

E

E

I

T

A

A

K

L

K

S

I

F

G

G

V

A

A

T

H

H

T

K

I

V

N

N

P

S

S

S

K

D

V

P

D

E

L

K

H

A

-

I

E

K

T

E

L

I

Q

Y

T

D

I

L

T

T

N

D

Q

G

D

R

G

G

P

V

D

D

V

V

I

A

I

I

E

E

A

A

V

V

G

G

S

I

P

P

T

A

T

T

Y

F

R

D

A

F

A

C

V

Q

E

K

E

I

V

I

A

G

F

V

T

D

G

G

R

T

V

V

V

A

C

N

I

C

G

G

Y

V

A

H

K

G

K

K

D

P

V

V

D

E

F

F

N

D

T

L

S

D

L

K

F

L

V

W

Q

I

K

R

E

N

I

I

E

N

I

V

L

T

G

T

S

G

R

L

N

V

C

S

L

T

G

N

D

T

F

T

P

P

I

Q

V

L

I

L

Q

K

Y

A

L

L

E

E

S

S

G

H

R

K

F

I

P

E

V

P

D

E

E

K

V

L

V

V

S

T

K

H

I

Y

V

F

S

K

I

L

D

S

E

E

active site: C41 (= C37), T43 (≠ G39), H46 (≠ N42), H63 (= H59)
binding zinc ion: C41 (= C37), H63 (= H59), C93 (= C90), C96 (= C93), C99 (= C96), C107 (= C104)

1r37A Alcohol dehydrogenase from sulfolobus solfataricus complexed with NAD(h) and 2-ethoxyethanol (see paper)
28% identity, 94% coverage: 1:320/340 of query aligns to 1:331/347 of 1r37A

query
sites
1r37A

M

M

K

R

A

A

L

V

V

R

I

L

T

V

E

E

V

I

G

G

K

K

-

P

T

L

E

S

I

L

Q

Q

E

E

V

I

E

G

K

V

P

P

N

K

I

P

S

K

S

G

E

P

E

Q

V

V

L

L

V

I

K

K

V

V

G

E

M

A

V

A

G

G

F

V

C
|
C

G
x
H

G
x
S

D

D

L

V

N
x
H

S

M

F

R

R

Q

G

G

L

R

F
|
F

-

G

P

N

L

L

Q

R

-

I

-

V

-

E

-

D

-

L

-

G

-

V

E

K

Y

L

P

P

N

V

I

T

I

L

G

G

H
|
H

E
|
E

V

I

G

A

G

G

T

K

I

I

E

E

A

E

I

V

G

G

E

D

N

E

V

V

P

V

D

-

S

G

L

Y

S

S

I

K

G

G

T

D

K

L

V

V

T

A

V

V

Y

N

P

P

Y

W

Q

Q

N

G

C
x
E

G

G

T

N

C
|
C

V

Y

A

Y

C
|
C

R

R

K

I

G

G

R

E

P

E

N

H

C

L

C
|
C

K

D

T

S

N

P

K
x
R

T

W

M

L

G

G

V

I

R

N

R

F

P

D

G

G

A

A

M

Y

T

A

R

E

Y

Y

I

V

A

I

V

V

P

P

-

H

Y

Y

Q

K

D

Y

V

M

F

Y

P

K

S

L

E

R

K

R

L

L

S

N

L

A

K

V

E

E

L

-

A

-

L

-

A

A

E

A

P

P

L

L

T

T

V
x
C

G

S

-

G

-

I

-
x
T

-

T

F

Y

H

R

A

A

A

V

E

R

R

K

G

A

R

S

V

L

N

D

-

P

-

T

E

K

E

T

D

L

C

L

V

V

A

V

V

G

L

A

G

G

C
x
G

G
|
G

I
x
L

V

G

G

T

M

M

G

A

A

V

I

Q

A

I

S

A

S

K

V

A

E

V

R

S

G

G

A

A

K

T

V

I

I

I

A

G

I

V

D
|
D

L
x
V

D
x
R

D

E

N

E

K

A

L

V

E

E

I

A

A

A

K

K

K

R

I

A

G

G

V

A

A

D

H

Y

T

V

I

I

N

N

P

A

S

S

K

M

V

Q

D

D

L

P

H

L

E

A

T

E

L

I

Q

R

T

R

I

I

T

T

N

E

Q

S

D

K

G

G

P

V

D

D

V

A

I

V

I

I

E

D

A
x
L

V
x
N

G

N

S

S

P

E

T

K

T

T

Y

L

R

S

A

V

A

Y

V

P

E

K

E

A

V

L

A

A

F

K

T

Q

G

G

R

K

V

Y

V

V

C

M

I
x
V

G
|
G

Y
x
L

A
x
F

K

G

K

A

D

D

V

L

D

H

F

Y

N

H

T

A

S

P

L

L

F

I

V

T

Q

L

K

S

E

E

I

I

E

Q

I

F

L

V

G

G

S

S

R
x
L

-
x
V

N

G

C

N

L

Q

G

S

D

D

F

F

P

L

I

G

V

I

I

M

Q

R

Y

L

L

A

E

E

S

A

G

G

R

K

F

-

P

-

V

V

D

K

E

P

V

M

V

I

S

T

K

K

I

T

V

M

S

K

I

L

D

E

E

E

A

A

P

N

Q

E

T

A

M

I

active site: C38 (= C37), H39 (≠ G38), S40 (≠ G39), H43 (≠ N42), H68 (= H59), E69 (= E60), E98 (≠ C90), C101 (= C93), C104 (= C96), C112 (= C104), R116 (≠ K108), C154 (≠ V148), T158 (vs. gap)
binding 2-ethoxyethanol: S40 (≠ G39), F49 (= F48)
binding nicotinamide-adenine-dinucleotide: C38 (= C37), H39 (≠ G38), S40 (≠ G39), H43 (≠ N42), C154 (≠ V148), T158 (vs. gap), G181 (≠ C169), G182 (= G170), L183 (≠ I171), D203 (= D191), V204 (≠ L192), R205 (≠ D193), L247 (≠ A235), N248 (≠ V236), V270 (≠ I258), G271 (= G259), L272 (≠ Y260), F273 (≠ A261), L295 (≠ R283), V296 (vs. gap)
binding zinc ion: C38 (= C37), H68 (= H59), E98 (≠ C90), C101 (= C93), C104 (= C96), C112 (= C104), C154 (≠ V148)

Sites not aligning to the query:

active site: 342
binding nicotinamide-adenine-dinucleotide: 334, 337, 342

1jvbA Alcohol dehydrogenase from the archaeon sulfolobus solfataricus (see paper)
28% identity, 94% coverage: 1:320/340 of query aligns to 1:331/347 of 1jvbA

query
sites
1jvbA

M

M

K

R

A

A

L

V

V

R

I

L

T

V

E

E

V

I

G

G

K

K

-

P

T

L

E

S

I

L

Q

Q

E

E

V

I

E

G

K

V

P

P

N

K

I

P

S

K

S

G

E

P

E

Q

V

V

L

L

V

I

K

K

V

V

G

E

M

A

V

A

G

G

F

V

C
|
C

G
x
H

G
x
S

D

D

L

V

N
x
H

S

M

F

R

R

Q

G

G

L

R

F

F

-

G

P

N

L

L

Q

R

-

I

-

V

-

E

-

D

-

L

-

G

-

V

E

K

Y

L

P

P

N

V

I

T

I

L

G

G

H
|
H

E
|
E

V

I

G

A

G

G

T

K

I

I

E

E

A

E

I

V

G

G

E

D

N

E

V

V

P

V

D

-

S

G

L

Y

S

S

I

K

G

G

T

D

K

L

V

V

T

A

V

V

Y

N

P

P

Y

W

Q

Q

N

G

C
x
E

G

G

T

N

C
|
C

V

Y

A

Y

C
|
C

R

R

K

I

G

G

R

E

P

E

N

H

C

L

C
|
C

K

D

T

S

N

P

K
x
R

T

W

M

L

G

G

V

I

R

N

R

F

P

D

G

G

A

A

M

Y

T

A

R

E

Y

Y

I

V

A

I

V

V

P

P

-

H

Y

Y

Q

K

D

Y

V

M

F

Y

P

K

S

L

E

R

K

R

L

L

S

N

L

A

K

V

E

E

L

-

A

-

L

-

A

A

E

A

P

P

L

L

T

T

V
x
C

G

S

-

G

-

I

-
x
T

-

T

F

Y

H

R

A

A

A

V

E

R

R

K

G

A

R

S

V

L

N

D

-

P

-

T

E

K

E

T

D

L

C

L

V

V

A

V

V

G

L

A

G

G

C

G

G

G

I

L

V

G

G

T

M

M

G

A

A

V

I

Q

A

I

S

A

S

K

V

A

E

V

R

S

G

G

A

A

K

T

V

I

I

I

A

G

I

V

D

D

L

V

D

R

D

E

N

E

K

A

L

V

E

E

I

A

A

A

K

K

K

R

I

A

G

G

V

A

A

D

H

Y

T

V

I

I

N

N

P

A

S

S

K

M

V

Q

D

D

L

P

H

L

E

A

T

E

L

I

Q

R

T

R

I

I

T

T

N

E

Q

S

D

K

G

G

P

V

D

D

V

A

I

V

I

I

E

D

A

L

V

N

G

N

S

S

P

E

T

K

T

T

Y

L

R

S

A

V

A

Y

V

P

E

K

E

A

V

L

A

A

F

K

T

Q

G

G

R

K

V

Y

V

V

C

M

I

V

G

G

Y

L

A

F

K

G

K

A

D

D

V

L

D

H

F

Y

N

H

T

A

S

P

L

L

F

I

V

T

Q

L

K

S

E

E

I

I

E

Q

I

F

L

V

G

G

S

S

R

L

-

V

N

G

C

N

L

Q

G

S

D

D

F

F

P

L

I

G

V

I

I

M

Q

R

Y

L

L

A

E

E

S

A

G

G

R

K

F

-

P

-

V

V

D

K

E

P

V

M

V

I

S

T

K

K

I

T

V

M

S

K

I

L

D

E

E

E

A

A

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N

Q

E

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A

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active site: C38 (= C37), H39 (≠ G38), S40 (≠ G39), H43 (≠ N42), H68 (= H59), E69 (= E60), E98 (≠ C90), C101 (= C93), C104 (= C96), C112 (= C104), R116 (≠ K108), C154 (≠ V148), T158 (vs. gap)
binding zinc ion: C38 (= C37), H68 (= H59), E69 (= E60), E98 (≠ C90), C101 (= C93), C104 (= C96), C112 (= C104), C154 (≠ V148)

Sites not aligning to the query:

active site: 342

P39462 NAD-dependent alcohol dehydrogenase; EC 1.1.1.1 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
28% identity, 94% coverage: 1:320/340 of query aligns to 1:331/347 of P39462

query
sites
P39462

M

M

K

R

A

A

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E

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K

-

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F

-

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N

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-

I

-

V

-

E

-

D

-

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-

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A

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K

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Y

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I

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N

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E

A

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L

A

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T

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K

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M

I

V

G

G

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L

A

F

K

G

K

A

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Y

N

H

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E

I

I

E

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-

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L

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M

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L

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A

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G

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-

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K11 (= K11) modified: N6-methyllysine; partial
K213 (= K201) modified: N6-methyllysine; partial

1e3jA Ketose reductase (sorbitol dehydrogenase) from silverleaf whitefly (see paper)
28% identity, 94% coverage: 20:339/340 of query aligns to 21:348/348 of 1e3jA

query
sites
1e3jA

P

P

N

E

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D

E

E

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A

M

Y

V

V

G

G

F

I

C
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C

G
|
G

G
x
S

D

D

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V

N
x
H

S

Y

F

Y

R

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-

H

-

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-

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-

I

G

A

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Q

K

E

D

Y

-

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M

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H
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H

E
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E

V

A

G

S

G

G

T

T

I

V

E

V

A

K

I

V

G

G

E

K

N

N

V

V

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-

D

K

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K

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D

K

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Y

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C
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C

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C
|
C

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Q

A

F

C
|
C

R

K

K

E

G

G

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Y

N

N

C

L

C
|
C

K

-

T

P

N

D

K

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T
|
T

M

F

G

C

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A

R

T

R

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P

P

-

D

G

G

A

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M

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T

A

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R

Y

Y

I

Y

-

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-

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A

A

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C

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-

E

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S

L

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E

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A

A

L

L

A

L

E

E

P
|
P

L

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G
|
G

F

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H

H

A

A

A

C

E

R

R

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N

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E

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E

G

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C

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V

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A

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G

G

C
x
A

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A

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G

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x
A

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x
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x
S

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K
x
R

L

L

E

E

I

V

A

A

K

K

K

N

I

C

G

G

-

A

-

D

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V

A

T

H

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I

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N

S

Q

A

-

I

-

G

D

D

G

L

P

P

D

N

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V

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T

I

I

E

D

A

C

V

S

G

G

S

N

P

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K

T

C

Y

I

R

T

A

I

A

G

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I

E

N

E

I

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T

A

R

F

T

T

G

G

G

R

T

V

L

V

M

C

L

I

V

G

G

Y

M

A

G

K

S

K

Q

D

M

V

V

D

T

F

V

N

P

T

L

S

V

L

N

F

A

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C

Q

A

K

R

E

E

I

I

E

D

I

I

L

K

G

S

S

V

R

F

N

R

C

Y

L

C

G

N

D

D

F

Y

P

P

I

I

V

A

I

L

Q

E

Y

M

L

V

E

A

S

S

G

G

R

R

F

C

P

N

V

V

D

K

E

Q

V

L

V

V

S

T

K

H

I

S

V

F

S

K

I

L

D

E

E

Q

A

T

P

V

Q

D

T

A

M

F

I

E

D

A

W

A

D

R

A

K

N

K

P

A

V

D

G

N

I

T

T

I

K
|
K

I

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M

M

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D

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C

D

R

Q

Q

active site: C38 (= C37), G39 (= G38), S40 (≠ G39), H43 (≠ N42), H63 (= H59), E64 (= E60), C93 (= C90), C96 (= C93), C99 (= C96), C107 (= C104), T111 (= T109), P150 (= P145), G154 (= G149), K341 (= K332)
binding phosphate ion: A174 (≠ C169), A196 (≠ D191), R197 (≠ L192), S198 (≠ D193), R201 (≠ K196)
binding zinc ion: C38 (= C37), H63 (= H59), E64 (= E60), C93 (= C90), C96 (= C93), C99 (= C96), C107 (= C104)

Query Sequence

>WP_008202270.1 NCBI__GCF_000166275.1:WP_008202270.1
MKALVITEVGKTEIQEVEKPNISSEEVLVKVGMVGFCGGDLNSFRGLFPLQEYPNIIGHE
VGGTIEAIGENVPDSLSIGTKVTVYPYQNCGTCVACRKGRPNCCKTNKTMGVRRPGAMTR
YIAVPYQDVFPSEKLSLKELALAEPLTVGFHAAERGRVNEEDCVAVLGCGIVGMGAIASS
VERGAKVIAIDLDDNKLEIAKKIGVAHTINPSKVDLHETLQTITNQDGPDVIIEAVGSPT
TYRAAVEEVAFTGRVVCIGYAKKDVDFNTSLFVQKEIEILGSRNCLGDFPIVIQYLESGR
FPVDEVVSKIVSIDEAPQTMIDWDANPVGITKIMIDFDQE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory