SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_008202270.1 NCBI__GCF_000166275.1:WP_008202270.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4ilkA Crystal structure of short chain alcohol dehydrogenase (rspb) from e. Coli cft073 (efi target efi-506413) complexed with cofactor nadh
30% identity, 99% coverage: 1:337/340 of query aligns to 4:337/337 of 4ilkA

query
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4ilkA

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active site: C40 (= C37), G41 (= G38), S42 (≠ G39), H45 (≠ N42), H59 (= H59), E60 (= E60), C89 (= C90), C92 (= C93), C95 (= C96), C103 (= C104), A107 (≠ K108), P145 (= P145), A149 (≠ G149), K332 (= K332)
binding manganese (ii) ion: C40 (= C37), H59 (= H59), E60 (= E60), E144 (= E144)
binding 1,4-dihydronicotinamide adenine dinucleotide: G168 (= G168), G170 (= G170), P171 (≠ I171), I172 (≠ V172), D193 (= D191), R194 (≠ L192), R198 (≠ K196), N213 (≠ P211), A235 (= A235), A236 (≠ V236), C237 (≠ G237), I241 (≠ T241), M258 (≠ I258), F260 (≠ Y260)
binding zinc ion: C89 (= C90), C92 (= C93), C95 (= C96), C103 (= C104)

7y9pA Xylitol dehydrogenase s96c/s99c/y102c mutant(thermostabilized form) from pichia stipitis (see paper)
34% identity, 87% coverage: 3:298/340 of query aligns to 5:310/357 of 7y9pA

query
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binding zinc ion: C40 (= C37), H65 (= H59), E66 (= E60), C95 (= C90), C98 (= C93), C101 (= C96), C109 (= C104)

5kiaA Crystal structure of l-threonine 3-dehydrogenase from burkholderia thailandensis
29% identity, 95% coverage: 1:323/340 of query aligns to 2:338/339 of 5kiaA

query
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5kiaA

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active site: C37 (= C37), G38 (= G38), T39 (≠ G39), H42 (≠ N42), H61 (= H59), E62 (= E60), C91 (= C90), C94 (= C93), C97 (= C96), C105 (= C104), V109 (≠ K108), P147 (= P145), A151 (≠ G149), K333 (≠ Q318)
binding calcium ion: D146 (≠ E144), N150 (≠ V148), E288 (≠ N284)
binding zinc ion: C91 (= C90), C94 (= C93), C97 (= C96), C105 (= C104)

2dfvA Hyperthermophilic threonine dehydrogenase from pyrococcus horikoshii (see paper)
32% identity, 91% coverage: 1:308/340 of query aligns to 3:317/346 of 2dfvA

query
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2dfvA

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R

S

G

G

A

A

-

Y

K

P

V

V

I

I

A

V

I

S

D
x
E

L
x
P

D

S

D

D

N

F

K
x
R

L

R

E

E

I

L

A

A

K

K

I

V

G

G

V

A

A

D

H

Y

T

V

I

I

N

N

P

P

S

F

K

E

V

E

D

D

L

V

H

V

E

K

T

E

L

V

Q

M

T

D

I

I

T

T

N

D

Q

G

D

N

G

G

P

V

D

D

V

V

I

F

I

L

E

E

A
x
F

V
x
S

G

G

S
x
A

P

P

T

K

T

A

Y

L

R

E

A

Q

A

G

V

L

E

Q

E

A

V

V

A

T

F

P

T

A

G

G

R

R

V

V

V

S

C

L

I
x
L

G
|
G

Y
x
L

-

Y

-

P

A

G

K

K

K

V

D

T

V

I

D

D

F

F

N

N

T

-

S

N

L

L

F

I

V

I

Q

F

K

K

E

A

I

L

E

T

I

I

L

Y

G

G

-
x
I

-
x
T

S

G

R

R

N

H

C

L

L

W

G

E

D

T

F

W

P

Y

I

T

V

V

I

S

Q

R

Y

L

L

L

E

Q

S

S

G

G

R

K

F

L

P

N

V

L

D

D

E

P

V

I

S

T

active site: C40 (= C37), G41 (= G38), T42 (≠ G39), H45 (≠ N42), H65 (= H59), E66 (= E60), C95 (= C90), C98 (= C93), C101 (= C96), C109 (= C104), K113 (= K108), P151 (= P145), A155 (≠ G149)
binding nicotinamide-adenine-dinucleotide: G175 (= G170), P176 (≠ I171), L177 (≠ V172), E197 (≠ D191), P198 (≠ L192), R202 (≠ K196), F241 (≠ A235), S242 (≠ V236), A244 (≠ S238), L264 (≠ I258), G265 (= G259), L266 (≠ Y260), I289 (vs. gap), T290 (vs. gap)
binding zinc ion: C95 (= C90), C101 (= C96), C109 (= C104)

Sites not aligning to the query:

active site: 340

O58389 L-threonine 3-dehydrogenase; L-ThrDH; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 2 papers)
32% identity, 91% coverage: 1:308/340 of query aligns to 5:319/348 of O58389

query
sites
O58389

M

M

K

V

A

A

L

I

V

M

I

K

T

T

E

K

V

P

G

G

K

Y

-

G

T

A

E

E

I

L

Q

V

E

E

V

V

E

D

K

V

P

P

N

K

I

P

S

G

S

P

E

G

E

E

V

V

L

L

V

I

K

K

V

V

G

L

M

A

V

T

G

S

F

I

C
|
C

G

G

G
x
T

D

D

L

L

N

H

S

-

F

-

R

-

G

-

L

I

F

Y

P

E

L

W

Q

N

E

E

Y

W

-

A

-

Q

-

S

-

R

-

I

-

K

-

P

P

P

N

Q

I

I

I

M

G

G

H
|
H

E
|
E

V

V

G

A

G

G

T

E

I

V

E

V

A

E

I

I

G

G

E

P

N

G

V

V

P

-

D

E

S

G

L

I

S

E

I

V

G

G

T

D

K

Y

V

V

T

S

V

V

Y

E

P

T

Y

H

Q

I

N

V

C
|
C

G

G

T

K

C
|
C

V

Y

A

A

C
|
C

R

R

K

R

G

G

R

Q

P

Y

N

H

C

V

C
|
C

K

Q

T

N

N

T

K

K

T

I

M

F

G

G

V

V

R

D

R

T

P

D

G

G

A

V

M

F

T

A

R

E

Y

Y

I

A

A

V

V

V

P

P

Y

A

Q

Q

D

N

V

I

F

W

P

K

S

N

E

P

K

K

L

S

S

I

L

P

K

P

E

E

L

Y

A

A

-

T

L

L

A

Q

E
|
E

P

P

L

L

T

G

V

N

G

A

F

V

H

D

A

T

A

V

E

L

R

A

G

G

R

P

V

I

N

S

E

G

E

K

D

S

C

-

V

V

A

L

V

I

L

T

G

G

C

A

G

G

I

P

V
x
L

G

G

M

L

G

L

A

G

I

I

A

A

S

V

S

A

V

K

E

A

R

S

G

G

A

A

-

Y

K

P

V

V

I

I

A

V

I

S

D
x
E

L

P

D

S

D

D

N

F

K
x
R

L

R

E

E

I

L

A

A

K

K

I

V

G

G

V

A

A

D

H

Y

T

V

I

I

N

N

P

P

S

F

K

E

V

E

D

D

L

V

H

V

E

K

T

E

L

V

Q

M

T

D

I

I

T

T

N

D

Q

G

D

N

G

G

P

V

D

D

V

V

I

F

I

L

E

E

A

F

V

S

G

G

S

A

P

P

T

K

T

A

Y

L

R

E

A

Q

A

G

V

L

E

Q

E

A

V

V

A

T

F

P

T

A

G

G

R

R

V

V

V

S

C

L

I
x
L

G
|
G

Y
x
L

-

Y

-

P

A

G

K

K

K

V

D

T

V

I

D

D

F

F

N

N

T

-

S

N

L

L

F

I

V

I

Q

F

K

K

E

A

I

L

E

T

I

I

L

Y

G

G

-
x
I

-
x
T

S

G

R
|
R

N

H

C

L

L

W

G

E

D

T

F

W

P

Y

I

T

V

V

I

S

Q

R

Y

L

L

L

E

Q

S

S

G

G

R

K

F

L

P

N

V

L

D

D

E

P

V

I

S

T

C42 (= C37) binding Zn(2+)
T44 (≠ G39) mutation to A: Total loss of enzymatic activity.
H67 (= H59) binding Zn(2+)
E68 (= E60) binding Zn(2+)
C97 (= C90) binding Zn(2+)
C100 (= C93) binding Zn(2+)
C103 (= C96) binding Zn(2+)
C111 (= C104) binding Zn(2+)
E152 (= E144) mutation E->A,Q: Almost complete loss of enzymatic activity.; mutation to C: 120-fold decrease in catalytic efficiency.; mutation to D: Shows 3-fold higher turnover rate and reduced affinities toward L-threonine and NAD(+), compared to wild-type.; mutation to K: Total loss of enzymatic activity.
L179 (≠ V172) binding NAD(+)
E199 (≠ D191) binding NAD(+); mutation to A: Large decrease in affinity for NAD(+).
R204 (≠ K196) binding NAD(+); mutation to A: Large decrease in affinity for NAD(+).
LGL 266:268 (≠ IGY 258:260) binding NAD(+)
IT 291:292 (vs. gap) binding NAD(+)
R294 (= R283) mutation to A: 4000-fold decrease in catalytic efficiency.

4uejA Closed state of galactitol-1-phosphate 5-dehydrogenase from e. Coli in complex with glycerol. (see paper)
33% identity, 83% coverage: 1:282/340 of query aligns to 1:286/346 of 4uejA

query
sites
4uejA

M

M

K

K

A

S

L

V

V

V

I

N

T

D

E

T

V

D

G

G

K

I

T

V

E

R

I

V

Q

A

E

E

V

S

E

V

K

I

P

P

N

E

I

I

S

K

S

H

E

Q

-

D

E

E

V

V

L

R

V

V

K

K

V

I

G

A

M

S

V

S

G

G

F

L

C
|
C

G
|
G

G
x
S

D

D

L

L

N
x
P

S

R

-

I

F

F

R

K

G

N

L

-

F

-

P

G

L

A

Q

H

E

Y

Y

Y

P

P

N

I

I

T

I

L

G

G

H
|
H

E
|
E

V

F

G

S

G

G

T

Y

I

I

E

D

A

A

I

V

G

G

E

S

N

G

V

V

P

-

D

D

S

D

L

L

S

H

I

P

G

G

T

D

K

A

V

V

T

A

V

C

Y

V

P

P

Y

L

Q

L

N

P

C
|
C

G

F

T

T

C
|
C

V

P

A

E

C
|
C

R

L

K

K

G

G

R

F

P

Y

N

S

C

Q

C
|
C

K

A

T

K

N

Y

K
x
D

T

F

M

I

G

G

V

S

R

R

P

D

G

G

A

G

M

F

T

A

R

E

Y

Y

I

I

A

V

V

V

P

K

Y

R

Q

K

D

N

V

V

F

F

-

A

-

L

P

P

S

T

E

D

K

-

L

M

S

P

L

I

K

E

E

D

L

G

A

A

L

F

A

I

E
|
E

P
|
P

L

I

T

T

V

V

G
|
G

F

L

H

H

A

A

A

F

E

H

R

L

G

A

R

Q

V

G

N

C

E

E

E

N

D

K

C

N

V

V

A

I

V

I

L

I

G

G

C

A

G

G

I

T

V

I

G

G

M

L

G

L

A

A

I

I

A

Q

S

C

S

A

V

V

E

A

R

L

G

G

A

A

K

K

-

S

V

V

I

T

A

A

I

I

D

D

L

I

D

S

N

E

K

K

L

L

E

A

I

L

A

A

K

K

K

S

I

F

G

G

V

A

A

M

H

Q

T

T

I

F

N

N

P

S

S

S

K

E

V

M

D

S

L

A

H

P

E

Q

T

M

L

Q

Q

S

T

V

I

L

T

R

N

E

Q

L

D

R

G

F

P

N

D

Q

V

L

I

I

I

L

E

E

A

T

V

A

G

G

S

V

P

P

T

Q

T

T

Y

V

R

E

A

L

A

A

V

V

E

E

E

I

V

A

A

G

F

P

T

H

G

A

R

Q

V

L

V

A

C

L

I

V

G

G

Y

T

A

L

K

H

K

Q

D

D

V

L

D

H

F

L

N

T

T

S

S

A

L

T

F

F

-

G

-

K

-

I

V

L

Q

R

K

K

E

E

I

L

E

T

I

V

L

I

G

G

S

S

active site: C38 (= C37), G39 (= G38), S40 (≠ G39), P43 (≠ N42), H59 (= H59), E60 (= E60), C89 (= C90), C92 (= C93), C95 (= C96), C103 (= C104), D107 (≠ K108), P145 (= P145), G149 (= G149)
binding glycerol: H59 (= H59), E144 (= E144)
binding zinc ion: C89 (= C90), C92 (= C93), C95 (= C96), C103 (= C104)

Sites not aligning to the query:

active site: 341

4a2cA Crystal structure of galactitol-1-phosphate dehydrogenase from escherichia coli (see paper)
33% identity, 83% coverage: 1:282/340 of query aligns to 1:286/346 of 4a2cA

query
sites
4a2cA

M

M

K

K

A

S

L

V

V

V

I

N

T

D

E

T

V

D

G

G

K

I

T

V

E

R

I

V

Q

A

E

E

V

S

E

V

K

I

P

P

N

E

I

I

S

K

S

H

E

Q

-

D

E

E

V

V

L

R

V

V

K

K

V

I

G

A

M

S

V

S

G

G

F

L

C
|
C

G
|
G

G
x
S

D

D

L

L

N
x
P

S

R

-

I

F

F

R

K

G

N

L

-

F

-

P

G

L

A

Q

H

E

Y

Y

Y

P

P

N

I

I

T

I

L

G

G

H
|
H

E
|
E

V

F

G

S

G

G

T

Y

I

I

E

D

A

A

I

V

G

G

E

S

N

G

V

V

P

-

D

D

S

D

L

L

S

H

I

P

G

G

T

D

K

A

V

V

T

A

V

C

Y

V

P

P

Y

L

Q

L

N

P

C
|
C

G

F

T

T

C
|
C

V

P

A

E

C
|
C

R

L

K

K

G

G

R

F

P

Y

N

S

C

Q

C
|
C

K

A

T

K

N

Y

K
x
D

T

F

M

I

G

G

V

S

R

R

P

D

G

G

A

G

M

F

T

A

R

E

Y

Y

I

I

A

V

V

V

P

K

Y

R

Q

K

D

N

V

V

F

F

-

A

-

L

P

P

S

T

E

D

K

-

L

M

S

P

L

I

K

E

E

D

L

G

A

A

L

F

A

I

E

E

P
|
P

L

I

T

T

V

V

G
|
G

F

L

H

H

A

A

A

F

E

H

R

L

G

A

R

Q

V

G

N

C

E

E

E

N

D

K

C

N

V

V

A

I

V

I

L

I

G

G

C

A

G

G

I

T

V

I

G

G

M

L

G

L

A

A

I

I

A

Q

S

C

S

A

V

V

E

A

R

L

G

G

A

A

K

K

-

S

V

V

I

T

A

A

I

I

D

D

L

I

D

S

N

E

K

K

L

L

E

A

I

L

A

A

K

K

K

S

I

F

G

G

V

A

A

M

H

Q

T

T

I

F

N

N

P

S

S

S

K

E

V

M

D

S

L

A

H

P

E

Q

T

M

L

Q

Q

S

T

V

I

L

T

R

N

E

Q

L

D

R

G

F

P

N

D

Q

V

L

I

I

I

L

E

E

A

T

V

A

G

G

S

V

P

P

T

Q

T

T

Y

V

R

E

A

L

A

A

V

V

E

E

E

I

V

A

A

G

F

P

T

H

G

A

R

Q

V

L

V

A

C

L

I

V

G

G

Y

T

A

L

K

H

K

Q

D

D

V

L

D

H

F

L

N

T

T

S

S

A

L

T

F

F

-

G

-

K

-

I

V

L

Q

R

K

K

E

E

I

L

E

T

I

V

L

I

G

G

S

S

active site: C38 (= C37), G39 (= G38), S40 (≠ G39), P43 (≠ N42), H59 (= H59), E60 (= E60), C89 (= C90), C92 (= C93), C95 (= C96), C103 (= C104), D107 (≠ K108), P145 (= P145), G149 (= G149)
binding zinc ion: C89 (= C90), C92 (= C93), C95 (= C96), C103 (= C104)

Sites not aligning to the query:

active site: 341

2eerB Structural study of project id st2577 from sulfolobus tokodaii strain7
28% identity, 94% coverage: 1:320/340 of query aligns to 1:331/347 of 2eerB

query
sites
2eerB

M

M

K

R

A

A

L

M

V

R

I

L

T

V

E

E

V

I

G

G

K

K

-

P

T

L

E

K

I

L

Q

E

E

D

V

I

E

P

K

I

P

P

N

K

I

P

S

K

S

G

E

S

E

Q

V

V

L

L

V

I

K

K

V

I

G

E

M

A

V

A

G

G

F

V

C
|
C

G
x
H

G
x
S

D

D

L

V

N
x
H

S

M

F

R

R

Q

G

G

L

R

F

F

-

G

P

N

L

L

Q

R

-

I

-

V

-

E

-

D

-

L

-

G

-

V

E

K

Y

L

P

P

N

V

I

T

I

L

G

G

H
|
H

E
|
E

V

I

G

A

G

G

T

R

I

I

E

E

A

E

I

V

G

G

E

D

N

E

V

V

P

V

D

-

S

G

L

Y

S

S

I

K

G

G

T

D

K

L

V

V

T

A

V

V

Y

N

P

P

Y

W

Q

E

N

G

C
x
E

G

G

T

N

C
|
C

V

Y

A

Y

C
|
C

R

R

K

I

G

G

R

E

P

E

N

H

C

L

C
|
C

K

D

T

S

N

P

K
x
R

T

W

M

L

G

G

V

I

R

N

R

Y

P

D

G

G

A

A

M

Y

T

A

R

E

Y

Y

I

V

A

L

V

V

P

P

-

H

Y

Y

Q

K

D

Y

V

L

F

Y

P

K

S

L

E

R

K

R

L

L

S

S

L

A

K

V

E

E

L

-

A

-

L

-

A

A

E

A

P

P

L

L

T

T

V
x
C

G

S

-

G

-

V

-
x
T

-

T

F

Y

H

R

A

A

A

V

E

R

R

K

G

A

R

S

V

L

N

D

E

P

E

S

D

K

C

T

V

L

A

V

V

V

L

I

G
|
G

C

A

G

G

I

-

V
x
G

G
|
G

M
x
L

G

G

A

T

I

M

A

A

S

I

S

Q

V

I

E

A

R

K

-

A

-

V

-

S

G

G

A

A

K

T

V

I

I

I

A

G

I

V

D
|
D

L
x
V

D
x
R

D

E

N

E

K

A

L

L

E

E

I

A

A

A

K

K

K

R

I

A

G

G

V

A

A

D

H

Y

T

V

I

I

N

N

P

A

S

S

K

S

V

Q

D

D

L

P

H

V

E

S

T

E

L

I

Q

R

T

R

I

I

T

T

N

Q

Q

G

D

K

G

G

P

A

D

D

V

A

I

V

I

I

E

D

A
x
L

V
x
N

G

N

S

S

P

E

T

K

T

T

Y

L

R

S

A

I

A

Y

V

P

E

Y

E

V

V

L

A

A

F

K

T

Q

G

G

R

K

V

Y

V

V

C

M

I
x
V

G
|
G

Y
x
L

A
x
F

K

G

K

A

D

D

V

L

D

K

F

Y

N

H

T

A

S

P

L

L

F

I

V

T

Q

L

K

N

E

E

I

V

E

Q

I

F

L

I

G

G

S

S

R
x
L

-
x
V

N

G

C

N

L

Q

G

S

D

D

F

F

P

L

I

G

V

I

I

M

Q

S

Y

L

L

A

E

E

S

A

G

G

R

K

F

-

P

-

V

V

D

K

E

P

V

M

V

V

S

T

K

K

I

T

V

M

S

K

I

L

D

E

E

E

A

A

P

N

Q

E

T

A

M

I

active site: C38 (= C37), H39 (≠ G38), S40 (≠ G39), H43 (≠ N42), H68 (= H59), E69 (= E60), E98 (≠ C90), C101 (= C93), C104 (= C96), C112 (= C104), R116 (≠ K108), C154 (≠ V148), T158 (vs. gap)
binding nicotinamide-adenine-dinucleotide: C38 (= C37), H39 (≠ G38), S40 (≠ G39), H43 (≠ N42), C154 (≠ V148), T158 (vs. gap), G178 (= G168), G181 (≠ V172), G182 (= G173), L183 (≠ M174), D203 (= D191), V204 (≠ L192), R205 (≠ D193), L247 (≠ A235), N248 (≠ V236), V270 (≠ I258), G271 (= G259), L272 (≠ Y260), F273 (≠ A261), L295 (≠ R283), V296 (vs. gap)
binding zinc ion: E98 (≠ C90), C101 (= C93), C104 (= C96), C112 (= C104)

Sites not aligning to the query:

active site: 342
binding nicotinamide-adenine-dinucleotide: 342

4cpdA Alcohol dehydrogenase tadh from thermus sp. Atn1
30% identity, 95% coverage: 1:324/340 of query aligns to 1:334/346 of 4cpdA

query
sites
4cpdA

M

M

K

R

A

A

L

V

V

V

I

F

T

E

E

N

V

K

G

E

K

R

T

V

E

A

I

V

Q

K

E

E

V

V

E

N

K

A

P

P

N

R

I

L

S

Q

S

H

E

P

-

L

E

D

V

A

L

L

V

V

K

R

V

V

G

H

M

L

V

A

G

G

F

I

C
|
C

G
|
G

G
x
S

D

D

L

L

N
x
H

S

L

F

Y

R

H

G

G

L

K

F

I

P

P

L

V

Q

L

E

P

Y

G

P

-

N

S

I

V

I

L

G

G

H
|
H

E
|
E

V

F

G

V

G

G

T

Q

I

V

E

E

A

A

I

V

G

G

E

E

N

G

V

I

P

Q

D

D

S

-

L

L

S

Q

I

P

G

G

T

D

K

W

V

V

T

V

V

G

Y

P

P

F

Y

H

Q

I

N

A

C
|
C

G

G

T

T

C
|
C

V

P

A

Y

C
|
C

R

R

K

R

G

H

R

Q

P

Y

N

N

C

L

C
|
C

K

E

T

R

N

G

K
x
G

T

V

M

Y

G

G

V

Y

-

G

-

P

-

M

-

F

-

G

R

N

R

L

P

Q

G

G

A

A

M

Q

T

A

R

E

Y

I

I

L

A

R

V

V

P

P

Y

F

Q

S

D

N

V

V

-

N

-

L

-

R

-

K

F

L

P

P

S

P

E

N

K

L

L

S

S

P

L

E

K

R

E

A

L

I

A

F

L

A

A

G

E
x
D

P

I

L

L

T

S

V
x
T

G

A

F

Y

H

G

A

G

A

L

E

I

R

Q

G

G

R

Q

V

L

N

R

E

P

E

G

D

D

C

S

V

V

A

A

V

V

L

I

G

G

C

A

G
|
G

I
x
P

V
|
V

G

G

M

L

G

M

A

A

I

I

A

E

S

V

S

A

V

Q

E

V

R

L

G

G

A

A

-

S

K

K

V

I

I

L

A

A

I

I

D
|
D

L
x
R

D

I

D

P

N

E

K
x
R

L

L

E

E

I

R

A

A

K

A

K

S

I

L

G

G

V

-

A

A

H

I

T

P

I

I

N

N

P

A

S

E

K

Q

V

E

D

N

L

P

H

V

E

R

T

R

L

V

Q

R

T

S

I

E

T

T

N

N

Q

D

D

E

G

G

P

P

D

D

V

L

I

V

I

L

E

E

A
|
A

V
|
V

G

G

S

G

P

A

T

A

T

T

Y

L

R

S

A

L

A

A

V

L

E

E

E

M

V

V

A

R

F

P

T

G

G

G

R

R

V

V

V

S

C

A

I
x
V

G

G

Y
x
V

A

D

K

N

K

A

-

P

D

S

V

F

D

P

F

F

N

P

T

L

S

A

L

S

F

G

V

L

Q

V

K

K

E

D

I

L

E

T

I

F

-

R

L

I

G

G

S
x
L

R
x
A

N

N

C

V

L

H

G

L

D

Y

F

I

P

D

I

A

V

V

I

L

Q

A

Y

L

L

L

E

A

S

S

G

G

R

R

F

L

P

Q

V

P

D

E

E

R

V

I

V

V

S

S

K

H

I

Y

V

L

S

P

I

L

D

E

E

E

A

A

P

P

Q

R

T

G

M

Y

I

E

D

L

W
x
F

D

D

active site: C38 (= C37), G39 (= G38), S40 (≠ G39), H43 (≠ N42), H59 (= H59), E60 (= E60), C89 (= C90), C92 (= C93), C95 (= C96), C103 (= C104), G107 (≠ K108), D152 (≠ E144), T156 (≠ V148)
binding nicotinamide-adenine-dinucleotide: G39 (= G38), S40 (≠ G39), T156 (≠ V148), G178 (= G170), P179 (≠ I171), V180 (= V172), D200 (= D191), R201 (≠ L192), R205 (≠ K196), A243 (= A235), V244 (= V236), V266 (≠ I258), V268 (≠ Y260), L292 (≠ S282), A293 (≠ R283), F333 (≠ W323)
binding zinc ion: C38 (= C37), H59 (= H59), C89 (= C90), C92 (= C93), C95 (= C96), C103 (= C104), D152 (≠ E144)

Sites not aligning to the query:

active site: 340

4ej6A Crystal structure of a putative zinc-binding dehydrogenase (target psi-012003) from sinorhizobium meliloti 1021
27% identity, 99% coverage: 1:335/340 of query aligns to 4:338/343 of 4ej6A

query
sites
4ej6A

M

M

K

K

A

A

L

V

V

R

I

L

T

E

E

S

V

V

G

G

K

N

T

I

E

S

I

V

Q

R

E

N

V

V

E

G

K

I

P

P

N

E

I

P

S

G

S

P

E

D

V

L

L

L

V

V

K

K

V

V

G

E

M

A

V

C

G

G

F

I

C
|
C

G
|
G

G
x
T

D

D

L

R

N
x
H

S

L

F

L

R

H

G

G

L

E

F

F

P

P

L

-

Q

S

E

T

Y

P

P

P

N

V

I

T

I

L

G

G

H
|
H

E
|
E

V

F

G

C

G

G

T

I

I

V

E

V

A

E

I

A

G

G

E

S

N

A

V

V

P

R

D

D

S

-

L

I

S

A

I

P

G

G

T

A

K

R

V

I

T

T

V

G

Y

D

P

P

Y

N

Q

I

N

S

C
|
C

G

G

T

R

C
|
C

V

P

A

Q

C
|
C

R

Q

K

A

G

G

R

R

P

V

N

N

C

L

C
|
C

K

R

T

N

N

L

K
x
R

T

A

M

I

G

G

V

I

R

H

R

R

P

D

G

G

A

G

M

F

T

A

R

E

Y

Y

I

V

A

L

V

V

P

P

Y

R

Q

K

D

Q

V

A

F

F

P

-

S

E

E

I

K

P

L

L

S

T

L

L

K

D

E

P

L

V

-

H

-

G

A

A

L

F

A

C

E

E

P
|
P

L

L

T

A

V

C

G
x
C

F

L

H

H

A

G

A

V

E

D

R

L

G

S

R

G

V

I

N

K

E

A

E

G

D

S

C

T

V

V

A

A

V

I

L

L

G

G

C

G

G

G

I

V

V

I

G

G

M

L

G

L

A

T

I

V

A

Q

S

L

S

A

V

R

E

L

R

A

G

G

A

A

K

T

V

T

I

V

A

I

I

L

D

S

L

T

D

R

D

Q

-

A

N

T

K

K

L

R

E

R

I

L

A

A

K

E

I

V

G

G

V

A

A

T

H

A

T

T

I

V

N

D

P

P

S

S

K

A

V

G

D

D

L

V

H

V

E

E

T

A

L

I

Q

A

T

G

I

P

T

V

N

G

-

L

-

V

Q

P

D

G

G

G

P

V

D

D

V

V

I

V

I

I

E

E

A

C

V

A

G

G

S

V

P

A

T

E

T

T

Y

V

R

K

A

Q

A

S

V

T

E

R

E

L

V

A

A

K

F

A

T

G

G

G

R

T

V

V

C

I

I

L

G

G

Y

V

A

L

K

P

K

Q

-

G

-

E

D

K

V

V

D

E

F

I

N

E

T

P

S

F

L

D

F

I

V

L

Q

F

K

R

E

E

I

L

E

R

I

V

L

L

G

G

S

S

R

F

N

I

C

N

L

P

G

F

D

V

F

H

P

R

I

R

V

A

I

A

Q

D

Y

L

L

V

E

A

S

T

G

G

R

A

F

I

P

E

V

I

D

D

E

R

V

M

V

I

S

S

K

R

I

R

V

I

S

S

I

L

D

D

E

E

A

A

P

P

Q

-

T

-

M

-

I

-

D

D

W

V

D

I

A

S

N

N

P

P

V

A

G

A

I

A

T

G

K

E

I

V

M
x
K

I

V

active site: C40 (= C37), G41 (= G38), T42 (≠ G39), H45 (≠ N42), H61 (= H59), E62 (= E60), C91 (= C90), C94 (= C93), C97 (= C96), C105 (= C104), R109 (≠ K108), P147 (= P145), C151 (≠ G149), K337 (≠ M334)
binding zinc ion: C91 (= C90), C94 (= C93), C97 (= C96), C105 (= C104)

4ejmA Crystal structure of a putative zinc-binding dehydrogenase (target psi-012003) from sinorhizobium meliloti 1021 bound to NADP
27% identity, 99% coverage: 1:335/340 of query aligns to 4:338/342 of 4ejmA

query
sites
4ejmA

M

M

K

K

A

A

L

V

V

R

I

L

T

E

E

S

V

V

G

G

K

N

T

I

E

S

I

V

Q

R

E

N

V

V

E

G

K

I

P

P

N

E

I

P

S

G

S

P

E

D

V

L

L

L

V

V

K

K

V

V

G

E

M

A

V

C

G

G

F

I

C
|
C

G
|
G

G
x
T

D

D

L

R

N
x
H

S

L

F

L

R

H

G

G

L

E

F

F

P

P

L

-

Q

S

E

T

Y

P

P

P

N

V

I

T

I

L

G

G

H
|
H

E
|
E

V

F

G

C

G

G

T

I

I

V

E

V

A

E

I

A

G

G

E

S

N

A

V

V

P

R

D

D

S

-

L

I

S

A

I

P

G

G

T

A

K

R

V

I

T

T

V

G

Y

D

P

P

Y

N

Q

I

N

S

C
|
C

G

G

T

R

C
|
C

V

P

A

Q

C
|
C

R

Q

K

A

G

G

R

R

P

V

N

N

C

L

C
|
C

K

R

T

N

N

L

K
x
R

T

A

M

I

G

G

V

I

R

H

R

R

P

D

G

G

A

G

M

F

T

A

R

E

Y

Y

I

V

A

L

V

V

P

P

Y

R

Q

K

D

Q

V

A

F

F

P

-

S

E

E

I

K

P

L

L

S

T

L

L

K

D

E

P

L

V

-

H

-

G

A

A

L

F

A

C

E

E

P
|
P

L

L

T

A

V

C

G
x
C

F

L

H

H

A

G

A

V

E

D

R

L

G

S

R

G

V

I

N

K

E

A

E

G

D

S

C

T

V

V

A

A

V

I

L

L

G
|
G

C

G

G
|
G

I
x
V

V
x
I

G

G

M

L

G

L

A

T

I

V

A

Q

S

L

S

A

V

R

E

L

R

A

G

G

A

A

K

T

V

T

I

V

A

I

I

L

D

S

L
x
T

D
x
R

D
x
Q

-

A

N

T

K
|
K

L

R

E

R

I

L

A

A

K

E

I

V

G

G

V

A

A

T

H

A

T

T

I

V

N

D

P

P

S

S

K

A

V

G

D

D

L

V

H

V

E

E

T

A

L

I

Q

A

T

G

I

P

T

V

N

G

-

L

-

V

Q

P

D

G

G

G

P

V

D

D

V

V

I

V

I

I

E

E

A
x
C

V

A

G

G

S

V

P

A

T
x
E

T
|
T

Y

V

R

K

A

Q

A

S

V

T

E

R

E

L

V

A

A

K

F

A

T

G

G

G

R

T

V

V

C

I

I
x
L

G

G

Y
x
V

A

L

K

P

K

Q

-

G

-

E

D

K

V

V

D

E

F

I

N

E

T

P

S

F

L

D

F

I

V

L

Q

F

K

R

E

E

I

L

E

R

I

V

L

L

G

G

S

S

R

F

N
x
I

C

N

L

P

G

F

D

V

F

H

P

R

I

R

V

A

I

A

Q

D

Y

L

L

V

E

A

S

T

G

G

R

A

F

I

P

E

V

I

D

D

E

R

V

M

V

I

S

S

K

R

I

R

V

I

S

S

I

L

D

D

E

E

A

A

P

P

Q

-

T

-

M

-

I

-

D

D

W

V

D

I

A

S

N

N

P

P

V

A

G

A

I

A

T

G

K

E

I

V

M
x
K

I

V

active site: C40 (= C37), G41 (= G38), T42 (≠ G39), H45 (≠ N42), H61 (= H59), E62 (= E60), C91 (= C90), C94 (= C93), C97 (= C96), C105 (= C104), R109 (≠ K108), P147 (= P145), C151 (≠ G149), K337 (≠ M334)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G170 (= G168), G172 (= G170), V173 (≠ I171), I174 (≠ V172), T194 (≠ L192), R195 (≠ D193), Q196 (≠ D194), K199 (= K196), C240 (≠ A235), E245 (≠ T240), T246 (= T241), L263 (≠ I258), V265 (≠ Y260), I291 (≠ N284)
binding zinc ion: C91 (= C90), C94 (= C93), C97 (= C96), C105 (= C104)

5vm2A Crystal structure of eck1772, an oxidoreductase/dehydrogenase of unknown specificity involved in membrane biogenesis from escherichia coli
30% identity, 96% coverage: 10:335/340 of query aligns to 12:343/347 of 5vm2A

query
sites
5vm2A

G

G

K

T

T

M

E

K

I

I

Q

I

E

S

V

A

E

E

K

I

P

P

N

V

I

P

S

K

S

E

E

D

E

E

V

V

L

L

V

I

K

K

V

V

G

E

M

Y

V

V

G

G

F

I

C
|
C

G
|
G

G
x
S

D

D

L

V

N
x
H

S

G

F

F

R

E

-

S

G

G

L

P

F

F

P

I

L

P

Q

P

E

K

Y

D

P

P

N

N

I

Q

-

E

-

I

-

G

I

L

G

G

H
|
H

E
|
E

V

C

G

A

G

G

T

T

I

V

E

V

A

A

I

V

G

G

E

S

N

R

V

V

P

-

D

R

S

K

L

F

S

K

I

P

G

G

T

D

K

R

V

V

T

N

V

I

Y

E

P

P

Y

G

Q

V

N

P

C
|
C

G

G

T

H

C
|
C

V

R

A

Y

C
|
C

R

L

K

E

G

G

R

K

P

Y

N

N

C

I

C
|
C

K

P

T

D

N

V

K
x
D

T

F

M

M

G

A

V

T

R

Q

-

P

-

N

R

Y

P

R

G

G

A

A

M

L

T

T

R

H

Y

Y

I

L

A

C

V

H

P

P

Y

E

Q

S

D

F

V

T

F

Y

P

K

-

L

S

P

E

D

K

N

L

M

S

D

L

T

K

M

E

E

L

G

A

T

L

L

A

V

E
|
E

P
|
P

L

A

T

A

V

V

G
|
G

F

M

H

H

A

A

E

M

R

L

G

A

R

D

V

V

N

K

E

P

E

G

D

K

C

K

V

I

A

I

V

I

L

L

G

G

C

A

G

G

I

C

V

I

G

G

M

L

G

M

A

T

I

L

A

Q

S

A

S

C

V

K

E

C

R

L

G

G

A

A

K

T

V

E

I

I

A

A

I

V

-

V

D

D

L

V

D

L

D

E

N

K

K

R

L

L

E

A

I

M

A

A

K

E

K

Q

I

L

G

G

V

A

A

T

H

V

T

V

I

I

N

N

P

G

S

A

K

K

V

E

D

D

L

T

H

I

E

A

T

R

L

C

Q

Q

T

Q

I

F

T

T

N

E

Q

D

D

M

G

G

P

A

D

D

V

I

I

V

I

F

E

E

A

T

V

A

G

G

S

S

P

A

T

V

T

T

Y

V

R

K

A

Q

A

A

V

P

E

Y

E

L

V

V

A

M

F

R

T

G

G

G

R

K

V

I

V

M

C

I

I

V

G

G

Y

T

A

V

K

P

K

G

D

A

V

S

D

A

F

I

N

N

T

F

S

-

L

L

F

K

V

I

Q

N

K

R

E

E

I

V

E

T

I

I

L

Q

G

T

S

V

R

F

N

R

C

Y

L

A

G

N

D

R

F

Y

P

P

I

V

V

T

I

I

Q

E

Y

A

L

I

E

S

S

S

G

G

R

R

F

F

P

D

V

V

D

K

E

S

V

M

V

V

S

T

K

H

I

I

V

Y

S

D

I

Y

D

R

E

D

A

V

P

Q

Q

Q

T

A

M

F

I

E

D

E

W

S

D

V

A

N

N

N

P

K

V

R

G

D

I

I

T

I

K
|
K

I

G

M

V

I

I

active site: C39 (= C37), G40 (= G38), S41 (≠ G39), H44 (≠ N42), H65 (= H59), E66 (= E60), C95 (= C90), C98 (= C93), C101 (= C96), C109 (= C104), D113 (≠ K108), P153 (= P145), G157 (= G149), K340 (= K332)
binding magnesium ion: H65 (= H59), E66 (= E60), E152 (= E144)
binding zinc ion: C95 (= C90), C98 (= C93), C101 (= C96), C109 (= C104)

P07913 L-threonine 3-dehydrogenase; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Escherichia coli (strain K12) (see paper)
25% identity, 91% coverage: 14:324/340 of query aligns to 15:341/341 of P07913

query
sites
P07913

I

M

Q

T

E

D

V

V

E

P

K

V

P

P

N

E

I

L

S

G

S

H

E

N

E

D

V

L

L

L

V

I

K

K

V

I

G

R

M

K

V

T

G

A

F

I

C
|
C

G

G

G

T

D

D

L

V

N

H

S

-

F

-

R

-

G

-

L

I

F

Y

P

N

L

W

Q

D

E

E

Y

W

-

S

-

Q

-

K

-

T

-

I

-

P

-

V

P

P

N

M

I

V

G

G

H

H

E

E

V

Y

G

V

G

G

T

E

I

V

E

V

A

G

I

I

G

G

E

Q

N

E

V

V

P

-

D

K

S

G

L

F

S

K

I

I

G

G

T

D

K

R

V

V

T

S

V

G

Y

E

P

G

Y

H

Q

I

N

T

C

C

G

G

T

H

C

C

V

R

A

N

C

C

R

R

K

G

G

G

R

R

P

T

N

H

C

L

C

C

K

R

T

N

N

T

K

I

T

G

M

V

G

G

V

V

R

N

R

R

P

P

G

G

A

C

M

F

T

A

R

E

Y

Y

I

L

A

V

V

I

P

P

Y

A

Q

F

D

N

V

A

F

F

P

K

-

I

S

P

E

D

K

N

L

I

S

S

L

D

K

D

E

L

L

A

A

A

L

I

A

F

E

D

P

P

L

F

T

G

V

N

G

A

F

V

H

H

A

T

A

A

E

L

R

S

G

F

R

D

V

L

N

V

E

G

E

E

D

D

C

-

V

V

A

L

V

V

L

S

G

G

C

A

G

G

I

P

V

I

G

G

M

I

G

M

A

A

I

A

A

A

S

V

S

A

V

K

E

H

R

V

G

G

A

A

K

R

-

N

V

V

I

V

A

I

I

T

D

D

L

V

D

N

D

E

N

Y

K

R

L

L

E

E

I

L

A

A

K

R

K

K

I

M

G

G

V

I

A

T

H

R

T

A

I

V

N

N

P

V

S

A

K

K

V

E

D

N

L

L

H

N

E

D

T

V

L

M

Q

A

T

E

I

L

T

G

N

M

Q

T

D

E

G

G

P

F

D

D

V

V

I

G

I

L

E

E

A

M

V

S

G

G

S

A

P

P

T

P

T

A

Y

F

R

R

A

T

A

M

V

L

E

D

E

T

V

M

A

N

F

H

T

G

G

G

R

R

V

I

V

A

C

M

I

L

G

G

Y

I

A

P

K

P

K

S

D

D

V

M

D

S

F

I

N

D

T

W

S

T

L

K

F

V

V

I

Q

F

K

K

E

G

I

L

E

F

I

I

L

K

G

G

-

I

-

Y

S

G

R

R

N

E

C

M

L

F

G

E

D

T

F

W

P

Y

I

K

V

M

I

A

Q

A

Y

L

L

I

E

Q

S

S

G

G

-

L

-

D

-

L

-

S