SitesBLAST
Comparing WP_008202643.1 NCBI__GCF_000166275.1:WP_008202643.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
2xiqA Crystal structure of human methylmalonyl-coa mutase in complex with adenosylcobalamin and malonyl-coa (see paper)
65% identity, 96% coverage: 23:703/706 of query aligns to 25:704/714 of 2xiqA
- active site: Y75 (= Y75), Y229 (= Y229), H230 (= H230), K586 (= K585), D590 (= D589), H592 (= H591)
- binding cobalamin: Y75 (= Y75), L105 (= L105), H108 (= H108), A125 (= A125), R193 (= R193), E233 (= E233), G320 (= G319), W321 (= W320), E357 (= E356), G360 (≠ A359), L361 (= L360), G591 (= G590), H592 (= H591), D593 (= D592), R594 (= R593), G595 (= G594), I599 (= I598), G635 (= G634), S637 (= S636), L639 (= L638), A641 (= A640), G667 (= G666), G668 (= G667), F687 (= F686), G688 (= G687), T691 (= T690)
- binding malonyl-coenzyme a: Y61 (= Y61), T63 (≠ S63), M64 (= M64), R68 (= R68), T71 (= T71), R73 (= R73), Y75 (= Y75), S150 (= S150), T152 (= T152), T181 (= T181), R193 (= R193), K220 (= K220), H230 (= H230), R269 (= R269), S271 (= S271), F273 (= F273), R313 (= R312), A314 (≠ T313), H315 (= H314), Q317 (= Q316), Q348 (= Q347)
P22033 Methylmalonyl-CoA mutase, mitochondrial; MCM; Methylmalonyl-CoA isomerase; EC 5.4.99.2 from Homo sapiens (Human) (see 28 papers)
65% identity, 96% coverage: 23:703/706 of query aligns to 60:739/750 of P22033
- I69 (≠ V32) to V: in MMAM; likely benign; dbSNP:rs115923556
- P86 (≠ A51) to L: in MMAM; mut0 and mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs769348060
- G87 (= G52) to E: in MMAM; mut0; dbSNP:rs1554160986
- R93 (= R58) to H: in MMAM; mut0; decreased methylmalonyl-CoA mutase activity; dbSNP:rs121918251
- G94 (= G59) to R: in MMAM; mut0; dbSNP:rs727504022; to V: in MMAM; mut- and mut0; dbSNP:rs535411418
- P95 (= P60) to R: in MMAM; mut0; dbSNP:rs190834116
- YPTM 96:99 (≠ YRSM 61:64) binding malonyl-CoA
- Y100 (= Y65) to C: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs864309735
- W105 (= W70) to R: in MMAM; mut0; dbSNP:rs121918249
- TIRQY 106:110 (= TIRQY 71:75) binding malonyl-CoA
- R108 (= R73) to C: in MMAM; mut0; dbSNP:rs121918257; to G: in MMAM; mut-; to H: in MMAM; mut0; dbSNP:rs483352778
- Q109 (= Q74) to R: in MMAM; mut0; dbSNP:rs1461110052
- G133 (= G98) to R: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs879253828
- A137 (= A102) to V: in MMAM; mut0; dbSNP:rs941483851
- D139 (= D104) to N: in MMAM; uncertain significance; dbSNP:rs879253829
- L140 (= L105) to P: in MMAM; decreased protein expression; decreased methylmalonyl-CoA mutase activity
- A141 (= A106) to T: in MMAM; decreased protein expression; dbSNP:rs1554160730
- H143 (= H108) to Y: in MMAM; mut0
- G145 (= G110) to S: in MMAM; mut0
- S148 (= S113) to L: in MMAM; mut0; dbSNP:rs1300547552
- D156 (= D121) to N: in MMAM; mut-
- G158 (= G123) to V: in MMAM; mut0
- G161 (= G126) to R: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; to V: in MMAM; decreased protein expression
- F174 (= F139) to S: in MMAM; mut0; dbSNP:rs864309733
- M186 (= M151) to V: in MMAM; mut-; dbSNP:rs148331800
- T187 (= T152) to S: in MMAM; mut0; dbSNP:rs879253830
- N189 (= N154) to I: in MMAM; mut-; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs200908035; to K: in MMAM; mut-; dbSNP:rs1561959114
- A191 (= A156) to E: in MMAM; mut- and mut0; affects proper folding; reduced protein level; decreased methylmalonyl-CoA mutase activity; dbSNP:rs760782399
- A197 (= A162) to E: in MMAM; mut0
- G203 (≠ A168) to R: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs778702777
- E205 (= E170) natural variant: Missing (in MMAM; mut0; dbSNP:rs879253831)
- G215 (= G180) to C: in MMAM; mut- and mut0; dbSNP:rs121918258; to S: in MMAM; mut0; dbSNP:rs121918258
- TIQ 216:218 (= TIQ 181:183) binding malonyl-CoA
- Q218 (= Q183) to H: in MMAM; mut0 and mut-; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; alters thermodynamic stability; dbSNP:rs1446389693
- N219 (= N184) to Y: in MMAM; mut0; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; dbSNP:rs121918256
- R228 (= R193) binding malonyl-CoA; to Q: in MMAM; mut0; dbSNP:rs770810987
- T230 (= T195) to I: in MMAM; mut-; to R: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs879253833
- Y231 (= Y196) to N: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; strong decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs864309736
- K255 (= K220) binding malonyl-CoA
- S262 (= S227) to N: in MMAM; mut0
- H265 (= H230) binding malonyl-CoA; to Y: in MMAM; mut-
- E276 (= E241) to D: in MMAM; uncertain significance; mut-; dbSNP:rs12175488
- L281 (= L246) to S: in MMAM; mut0; dbSNP:rs796052007
- G284 (= G249) to E: in MMAM; mut0; dbSNP:rs879253835; to R: in MMAM; mut0; dbSNP:rs761477436
- S288 (≠ L253) to P: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs1179778233
- G291 (= G256) to E: in MMAM; mut0
- Q293 (≠ K258) to P: in MMAM; mut0
- RLS 304:306 (= RLS 269:271) binding malonyl-CoA
- L305 (= L270) to S: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs1554160246
- S306 (= S271) to F: in MMAM; mut0; dbSNP:rs1085307929
- W309 (= W274) to G: in MMAM; decreased protein expression
- G312 (= G277) to V: in MMAM; mut0; dbSNP:rs864309734
- Y316 (≠ F281) to C: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; no decreased affinity for adenosylcob(III)alamin; dbSNP:rs781474200
- A324 (= A289) to T: in MMAM; mut-; dbSNP:rs780387525
- R326 (= R291) to K: in MMAM; uncertain significance; dbSNP:rs758577372
- L328 (= L293) to F: in MMAM; mut0; affects proper folding; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; dbSNP:rs796052002; to P: in MMAM; mut0; dbSNP:rs965316043
- S344 (= S308) to F: in MMAM; mut-; affects proper folding; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin
- L346 (≠ A310) natural variant: Missing (in MMAM; mut0)
- L347 (= L311) to R: in MMAM; mut0; dbSNP:rs1026703654
- H350 (= H314) to Y: in MMAM; mut0; dbSNP:rs1407914109
- L358 (= L322) to P: in MMAM; mut0
- N366 (= N330) to S: in MMAM; mut-; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs864309737
- R369 (= R333) to C: in MMAM; mut0; dbSNP:rs772552898; to H: in MMAM; mut- and mut0; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; alters thermodynamic stability; dbSNP:rs564069299
- T370 (= T334) to P: in MMAM; mut0; dbSNP:rs368790885
- A377 (= A341) to E: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs121918250
- Q383 (= Q347) to H: in MMAM; mut0; to P: in MMAM; mut0
- H386 (= H350) to N: in MMAM; mut0; dbSNP:rs1554159937; to R: in MMAM; mut0; dbSNP:rs866933356
- T387 (= T351) to I: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability
- N388 (= N352) to H: in MMAM; mut0; dbSNP:rs766010704; to K: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs879253840
- S389 (= S353) natural variant: Missing (in MMAM; mut0)
- I412 (≠ L376) natural variant: Missing (in MMAM; mut0)
- P424 (= P388) to L: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs879253842
- G426 (= G390) to E: in MMAM; mut-; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs533755473; to R: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; strong decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs769922244
- G427 (= G391) to D: in MMAM; mut0; dbSNP:rs753288303
- G454 (= G418) to E: in MMAM; mut0
- A499 (≠ E463) to T: in dbSNP:rs2229385
- I505 (≠ V469) to T: in MMAM; decreased protein expression; decreased methylmalonyl-CoA mutase activity
- Q514 (= Q478) to E: in MMAM; uncertain significance; to K: in MMAM; decreased protein expression
- L518 (= L482) to P: in MMAM; mut0; dbSNP:rs864309738
- R532 (≠ K496) to H: in dbSNP:rs1141321
- A535 (≠ Q499) to P: in MMAM; mut0; dbSNP:rs760183775
- A552 (= A516) to V: in MMAM; uncertain significance; dbSNP:rs879253845
- C560 (≠ A524) to Y: in MMAM; mut0; dbSNP:rs1238333040
- T566 (≠ S530) to R: in MMAM; mut0
- F573 (= F537) to S: in MMAM; mut-; affects proper folding; no effect on protein abundance; no effect on methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; does not alter thermodynamic stability; dbSNP:rs775593146
- Y587 (= Y551) to C: in MMAM; mut-
- I597 (≠ F561) to R: in MMAM; no changed in protein expression; decreased methylmalonyl-CoA mutase activity; dbSNP:rs1554158951
- P615 (= P579) to L: in MMAM; mut0; affects proper folding; reduced strongly protein level; to R: in MMAM; mut0; dbSNP:rs1554158777; to T: in MMAM; mut0; affects proper folding; reduced strongly protein level; loss of methylmalonyl-CoA mutase activity; dbSNP:rs1302409621
- R616 (= R580) to C: in MMAM; mut0; dbSNP:rs765284825
- L617 (≠ I581) to R: in MMAM; mut0; dbSNP:rs1554158775
- K621 (= K585) to N: in MMAM; mut0
- G623 (= G587) to R: in MMAM; mut0; dbSNP:rs121918254
- Q624 (= Q588) to R: in MMAM; no effect on protein abundance; dbSNP:rs768521956
- D625 (= D589) to G: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs879253847; to V: in MMAM; mut0; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity
- G626 (= G590) to C: in MMAM; mut-; dbSNP:rs982110849
- H627 (= H591) binding axial binding residue; to R: in MMAM; mut0; dbSNP:rs372486357
- G630 (= G594) to E: in MMAM; mut0; dbSNP:rs143023066
- V633 (= V597) to G: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; does not alter thermodynamic stability; dbSNP:rs200055428
- G637 (= G601) to E: in MMAM; mut-; to R: in MMAM; mut0; dbSNP:rs781501004
- F638 (= F602) to I: in MMAM; mut0
- D640 (= D604) to Y: in MMAM; mut0; dbSNP:rs865815395
- G642 (= G606) to R: in MMAM; mut-; dbSNP:rs747897332
- G648 (= G612) to D: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; strong decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs766721811
- V669 (= V633) to E: in MMAM; mut0; dbSNP:rs1360470463
- I671 (≠ A635) to V: in dbSNP:rs8589
- L674 (= L638) to F: in MMAM; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs1164271240
- H678 (= H642) to R: in MMAM; mut-; dbSNP:rs147094927
- E684 (≠ Q648) natural variant: E -> EL (in MMAM; mut-)
- L685 (= L649) to R: in MMAM; mut-; dbSNP:rs864309739
- R694 (= R658) to L: in MMAM; mut-; decreased protein abundance; loss of methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; to W: in MMAM; mut- and mut0; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs777758903
- M700 (≠ V664) to K: in MMAM; mut-; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs140600746
- G703 (= G667) to R: in MMAM; mut0; dbSNP:rs121918255
- G717 (= G681) to V: in MMAM; mut-; no effect on protein abundance; interferes with the binding of the cofactor to the apoenzyme; decreased methylmalonyl-CoA mutase activity; strong decreased affinity for adenosylcob(III)alamin; decreased thermodynamic stability; dbSNP:rs121918252
- G723 (= G687) to D: in MMAM; decreased protein expression; decreased methylmalonyl-CoA mutase activity; dbSNP:rs755077681
Sites not aligning to the query:
- 1:32 modified: transit peptide, Mitochondrion
- 7:750 natural variant: Missing (in MMAM; mut-)
- 50 binding malonyl-CoA
- 152:750 natural variant: Missing (in MMAM; mut0)
- 228:750 natural variant: Missing (in MMAM; mut0)
8dyjB Crystal structure of human methylmalonyl-coa mutase in complex with adp and cob(ii)alamin (see paper)
65% identity, 96% coverage: 23:703/706 of query aligns to 24:703/708 of 8dyjB
- binding adenosine-5'-diphosphate: Y74 (= Y75), T151 (= T152), R192 (= R193), Y228 (= Y229), H229 (= H230), F272 (= F273), Q316 (= Q316), N352 (= N352), E356 (= E356), L360 (= L360), P361 (= P361)
- binding cobalamin: F102 (= F103), L104 (= L105), H107 (= H108), A124 (= A125), V191 (= V192), R192 (= R193), H229 (= H230), E232 (= E233), G319 (= G319), W320 (= W320), E356 (= E356), G359 (≠ A359), L360 (= L360), G590 (= G590), H591 (= H591), D592 (= D592), R593 (= R593), G594 (= G594), I598 (= I598), S636 (= S636), L638 (= L638), A640 (= A640), G666 (= G666), G667 (= G667), V668 (= V668), F686 (= F686), G687 (= G687), T690 (= T690)
6reqA Methylmalonyl-coa mutase, 3-carboxypropyl-coa inhibitor complex (see paper)
66% identity, 96% coverage: 24:703/706 of query aligns to 37:721/727 of 6reqA