SitesBLAST

1a56A Primary sequence and solution conformation of ferricytochromE C-552 from nitrosomonas europaea, nmr, mean structure refined with explicit hydrogen bond constraints (see paper)
45% identity, 54% coverage: 67:142/142 of query aligns to 6:81/81 of 1a56A

query
sites
1a56A

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binding heme c: N9 (≠ D70), C10 (= C71), C13 (= C74), H14 (= H75), V21 (= V82), G22 (= G83), P23 (= P84), K45 (= K106), I46 (≠ V107), S50 (≠ G111), W54 (= W115), Q56 (≠ E117), I57 (≠ V118), M59 (= M120), N62 (≠ H123), V63 (≠ P124), N64 (≠ T125)

5xecA Heterodimer constructed from pa cyt c551-ht cyt c552 and ht cyt c552- pa cyt c551 chimeric proteins (see paper)
44% identity, 54% coverage: 66:142/142 of query aligns to 7:82/82 of 5xecA

query
sites
5xecA

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binding heme c: C12 (= C71), C15 (= C74), H16 (= H75), G24 (= G83), P25 (= P84), Y27 (= Y86), Y34 (= Y93), Y43 (≠ K102), I48 (≠ V107), S53 (≠ V112), G54 (= G113), V55 (= V114), W56 (= W115), G57 (= G116), V59 (= V118), M61 (= M120), Q64 (≠ H123), I78 (≠ V138)

P00099 Cytochrome c-551; Cytochrome C8; Cytochrome c551 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 4 papers)
41% identity, 52% coverage: 66:139/142 of query aligns to 29:101/104 of P00099

query
sites
P00099

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H38 (= H75) binding axial binding residue
M83 (= M120) binding axial binding residue

Sites not aligning to the query:

1:22 signal peptide
23:104 modified: mature protein, Cytochrome c-551

351cA Structure of cytochrome c551 from p. Aeruginosa refined at 1.6 angstroms resolution and comparison of the two redox forms (see paper)
40% identity, 54% coverage: 66:142/142 of query aligns to 7:82/82 of 351cA

query
sites
351cA

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binding protoporphyrin ix containing fe: C12 (= C71), C15 (= C74), H16 (= H75), G24 (= G83), P25 (= P84), Y27 (= Y86), L44 (= L103), R47 (≠ K106), I48 (≠ V107), Q53 (≠ V112), G54 (= G113), V55 (= V114), W56 (= W115), G57 (= G116), I59 (≠ V118), P60 (= P119), M61 (= M120)

1corA Investigation of the solution conformation of cytochromE C-551 from pseudomonas stutzeri (see paper)
40% identity, 57% coverage: 62:142/142 of query aligns to 3:82/82 of 1corA

query
sites
1corA

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binding heme c: K10 (≠ S69), C12 (= C71), C15 (= C74), H16 (= H75), V23 (= V82), G24 (= G83), P25 (= P84), L44 (= L103), H47 (≠ K106), I48 (≠ V107), S52 (≠ G111), Q53 (≠ V112), G54 (= G113), V55 (= V114), W56 (= W115), G57 (= G116), I59 (≠ V118), M61 (= M120), N64 (≠ H123)

2exvA Crystal structure of the f7a mutant of the cytochrome c551 from pseudomonas aeruginosa (see paper)
41% identity, 54% coverage: 67:142/142 of query aligns to 8:82/82 of 2exvA

query
sites
2exvA

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binding heme c: C12 (= C71), C15 (= C74), H16 (= H75), G24 (= G83), P25 (= P84), R47 (≠ K106), I48 (≠ V107), S52 (≠ G111), Q53 (≠ V112), G54 (= G113), V55 (= V114), W56 (= W115), G57 (= G116), I59 (≠ V118), P60 (= P119), M61 (= M120), N64 (≠ H123)

6kq1A Crystal structure of cytochrome c551 from pseudomonas sp. Strain mt-1.
42% identity, 57% coverage: 62:142/142 of query aligns to 3:82/82 of 6kq1A

query
sites
6kq1A

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binding heme c: C12 (= C71), C15 (= C74), H16 (= H75), G24 (= G83), P25 (= P84), V30 (= V89), Y34 (= Y93), L44 (= L103), H47 (≠ K106), I48 (≠ V107), Q53 (≠ V112), G54 (= G113), N55 (≠ V114), W56 (= W115), G57 (= G116), I59 (≠ V118), M61 (= M120), N64 (≠ H123)
binding zinc ion: E4 (≠ L63), K10 (≠ S69), E70 (≠ D130)

Sites not aligning to the query:

binding zinc ion: 2

5aurA Hydrogenobacter thermophilus cytochrome c552 dimer formed by domain swapping at n-terminal region (see paper)
43% identity, 54% coverage: 67:142/142 of query aligns to 6:83/83 of 5aurA

query
sites
5aurA

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binding heme c: C10 (= C71), C13 (= C74), H14 (= H75), P26 (= P84), Y28 (= Y86), Y35 (= Y93), Y44 (≠ K102), I49 (≠ V107), S54 (≠ V112), G55 (= G113), V56 (= V114), W57 (= W115), G58 (= G116), V60 (= V118), P61 (= P119), M62 (= M120), Q65 (≠ H123)

1cchA The solution conformation of cytochromE C-551 from p.Stutzeri zobell determined by nmr+ (see paper)
43% identity, 57% coverage: 62:142/142 of query aligns to 3:82/82 of 1cchA

query
sites
1cchA

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binding heme c: C12 (= C71), C15 (= C74), H16 (= H75), L44 (= L103), H47 (≠ K106), I48 (≠ V107), S52 (≠ G111), Q53 (≠ V112), V55 (= V114), W56 (= W115), G57 (= G116), I59 (≠ V118), M61 (= M120), P62 (= P121), N64 (≠ H123)

5xecC Heterodimer constructed from pa cyt c551-ht cyt c552 and ht cyt c552- pa cyt c551 chimeric proteins (see paper)
41% identity, 54% coverage: 67:142/142 of query aligns to 6:80/80 of 5xecC

query
sites
5xecC

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binding heme c: C10 (= C71), C13 (= C74), H14 (= H75), P23 (= P84), Y25 (= Y86), L42 (= L103), R45 (≠ K106), Q51 (≠ V112), V53 (= V114), W54 (= W115), G55 (= G116), I57 (≠ V118), M59 (= M120), N62 (≠ H123)

Query Sequence

>WP_008202837.1 NCBI__GCF_000166275.1:WP_008202837.1
MKITKPLNLGLAALAGLAFACGGGEKSTETTTTPAVESTPAEPAQEMSLEEKYKDDPIYI
KGLAKLKTSDCTTCHMVERKIVGPSYADVAAKYESTEENIEKLAQKVIEGGVGVWGEVPM
PPHPTLSMEDAKDMVAYVLLLK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory