SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_008203192.1 NCBI__GCF_000166275.1:WP_008203192.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1yiqA Molecular cloning and structural analysis of quinohemoprotein alcohol dehydrogenase adhiig from pseudomonas putida hk5. Compariison to the other quinohemoprotein alcohol dehydrogenase adhiib found in the same microorganism. (see paper)
29% identity, 66% coverage: 15:480/705 of query aligns to 12:430/684 of 1yiqA

query
sites
1yiqA

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active site: E178 (= E201), N255 (≠ S270), D300 (= D313)
binding calcium ion: E178 (= E201), N255 (≠ S270), D300 (= D313)
binding pyrroloquinoline quinone: E63 (≠ Q74), C109 (≠ S118), C110 (≠ W119), R115 (= R124), T160 (≠ A182), G175 (≠ R198), G176 (≠ L199), A177 (≠ S200), E178 (= E201), T235 (≠ N250), W237 (= W252), K327 (= K340), D390 (≠ E440), W391 (= W441)

Sites not aligning to the query:

binding heme c: 605, 606, 608, 609, 610, 623, 626, 630, 634, 637, 638, 642, 645, 646, 647, 648, 650
binding pyrroloquinoline quinone: 477, 542

Q4W6G0 Quinohemoprotein alcohol dehydrogenase ADH-IIG; ADH IIG; Alcohol dehydrogenase (azurin); EC 1.1.9.1 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
29% identity, 66% coverage: 15:480/705 of query aligns to 41:459/718 of Q4W6G0

query
sites
Q4W6G0

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C138 (≠ S118) modified: Disulfide link with 139
C139 (≠ W119) modified: Disulfide link with 138
R144 (= R124) binding pyrroloquinoline quinone
T189 (≠ A182) binding pyrroloquinoline quinone
GA 205:206 (≠ LS 199:200) binding pyrroloquinoline quinone
E207 (= E201) binding Ca(2+)
T264 (≠ N250) binding pyrroloquinoline quinone
N284 (≠ S270) binding Ca(2+)
D329 (= D313) binding Ca(2+)
K356 (= K340) binding pyrroloquinoline quinone
W415 (≠ D436) binding substrate
DW 419:420 (≠ EW 440:441) binding pyrroloquinoline quinone

Sites not aligning to the query:

1:29 signal peptide
30:718 modified: mature protein, Quinohemoprotein alcohol dehydrogenase ADH-IIG
635 binding covalent
638 binding covalent
639 binding axial binding residue
676 binding axial binding residue

O05542 Alcohol dehydrogenase (quinone), dehydrogenase subunit; ADH; Alcohol dehydrogenase (quinone), acceptor subunit; Alcohol dehydrogenase (quinone), subunit I; Ethanol:Q2 reductase; G3-ADH subunit I; Quinohemoprotein alcohol dehydrogenase; Quinohemoprotein-cytochrome c complex; Ubiquinol oxidase; EC 1.1.5.5 from Gluconobacter oxydans (strain 621H) (Gluconobacter suboxydans) (see paper)
27% identity, 64% coverage: 17:465/705 of query aligns to 41:456/757 of O05542

query
sites
O05542

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G

Q

K

L

L

A

K

Q

L

A

A

W

W

V

H

Y

Y

H

D

T

L

G

D

D

-

A

-

T

T

E

N

R

R

S

G

Q

Q

I

-

Q

E

C

G

Q

T

P

P

I

L

V

I

I

V

D

N

G

G

I

V

L

M

Y

Y

G

A

T

T

S

T

P

N

S

W

L

S

D

K

V

M

F

K

A

A

L

L

D

D

A

A

T

T

G

G

K

K

E

L

I

L

W

W

R

S

F

Y

D

D

P

P

F

K

-

V

-

P

D

G

I

N

L

I

G

A

G

D

E

R

N

G

S

C

W

C

A

D

G

T

T

V

N

S

R

R

G

G

V

A

S

A

Y

Y

W

W

A

-

E

-

A

-

E

-

D

N

K

G

R

K

I

V

L

Y

F

F

G

G

A

T

G

F

N

D

-

G

W

R

L

L

M

I

A

A

V

L

D

D

A

A

L

K

T

T

G

G

N

K

P

L

I

V

S

W

E

S

F

V

G

-

D

-

G

-

K

-

V

Y

D

T

L

I

R

P

K

K

N

E

L

A

D

Q

T

L

D

G

R

H

D

Q

D

R

F

S

L

Y

I

T

V

V

A

D

N

G

A

A

P

P

G

-

V

R

I

I

F

A

K

K

D

G

K

K

I

V

I

L

I

I

G

G

M

N

R

G

L

G

S

A

E

E

G

F

L

-

D

-

A

G

A

A

P

R

G

G

H

F

I

V

R

S

A

A

Y

F

N

D

V

A

K

E

T

T

G

G

K

K

R

L

E

D

W

W

I

R

F

F

H

F

T

T

I

V

P

P

Q

N

K

P

G

E

-

N

-

K

-

P

-

D

-

G

-

A

-

S

-

D

-

I

-

L

-

M

E

S

F

K

G

A

Y

Y

D

P

T

T

W

W

-

G

-

K

D

N

S

G

E

A

F

W

I

K

Q

Q

K

Q

I

G

G

G

A

G

N

T

N

V

W

W

A

D

G

S

M

L

V

V

V

Y

D

D

Q

P

K

V

R

T

E

D

I

L

V

V

F

Y

V

L

P

G

T

V

G

G

S

N

A

G

T

S

-

P

-

W

-

N

Y

Y

D

K

F

F

W

R

G

S

G

E

Y

G

R

K

T

-

G

G

D

D

N

N

L

L

Y

F

A

L

N

G

S

S

L

I

V

V

A

A

L

I

D

N

A

P

N

D

T

T

G

G

E

K

R

Y

I

V

W

W

H

H

F

F

Q

Q

A

E

V

T

H

P

H

M

D

D

V

E

W

W

D

D

R

Y

D

T

F

S

P

V

A

Q

N

Q

P

I

N

M

L

T

I

L

R

D

I

M

Q

P

K

V

E

N

G

G

E

E

W

M

I

R

D

H

A

V

V

I

A

V

Q

H

I

A

S

P

K

K

Q

N

G

G

M

F

T

F

Y

Y

V

I

F

I

D

D

R

A

E

K

S

T

G

G

E

K

P

F

I

I

W

-

P

-

I

-

V

-

E

-

T

-

N

-

V

-

P

-

Q

-

S

-

N

-

L

-

P

-

G

-

E

-

I

-

T

-

S

-

A

-

T

-

Q

-

P

-

I

-

P

-

T

-

L

T

P

G

E

K

P

P

F

Y

M

T

N

Y

M

E

T

N

F

W

E

A

D

N

S

G

-

L

D

D

I

P

L

V

S

T

L

G

K

R

P

P

E

N

W

Y

E

V

E

P

D

D

I

A

R

L

S

W

Q

T

L

L

V

T

N

-

V

-

Q

-

Y

-

G

G

D

K

T

P

W

W

A

-

P

-

S

-

E

-

H

-

G

-

I

-

I

L

L

G

F

I

P

P

G

G

M

E

D

L

G

G

G

H

E

N

W

F

G

A

A

M

S

A

F

Y

D

S

P

P

S

K

N

T

Q

K

T

L

L

V

Y

Y

V

I

N

P

A

A

N

Q

Q

Q

I

I

P

P

W

L

L

L

I

Y

E

D

Sites not aligning to the query:

1:34 signal peptide
35 modified: Pyrrolidone carboxylic acid

Q8GR64 Quinohemoprotein alcohol dehydrogenase ADH IIB; ADH IIB; Alcohol dehydrogenase (azurin); EC 1.1.9.1 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 3 papers)
27% identity, 67% coverage: 29:498/705 of query aligns to 39:455/690 of Q8GR64

query
sites
Q8GR64

D
x
E

W
|
W

S
x
L

H
x
S

Y
x
H

G
|
G

G
x
R

P
x
T

E
x
Y

D
x
A

G
x
E

S
x
Q

R
|
R

Y
x
F

S
|
S

S
x
P

L
|
L

T
x
K

Q
|
Q

I
|
I

N
x
D

K
x
A

D
x
S

N
|
N

V
|
V

S
x
R

Q
x
S

L
|
L

A
x
G

Q
x
L

A
|
A

W
|
W

V
x
Y

Y
x
M

H

-

T

-

G

-

D
|
D

A
x
L

T
x
D

E
x
N

R
x
T

S
x
R

Q
x
G

I
x
L

Q
x
E

C
x
A

Q
x
T

P
|
P

I
x
L

V
x
F

I
x
H

D
|
D

G
|
G

I
x
V

L
x
I

Y
|
Y

G
x
T

T
x
S

S
x
M

P
x
S

S
x
W

L
x
S

D
x
R

V
|
V

F
x
I

A
|
A

L
x
V

D
|
D

A
|
A

T
x
S

G
|
G

K
|
K

E
|
E

I
x
L

W
|
W

R
|
R

F
x
Y

D
|
D

P
|
P

-
x
E

-
x
V

F
x
A

D
x
K

I
x
V

L
x
K

G
x
A

G
x
R

E
x
T

N
x
S

S
x
C

W
x
C

A
x
D

G
x
A

T
x
V

N
|
N

R
|
R

G
|
G

V
|
V

S
x
A

Y
x
L

W
|
W

A
x
G

E

-

A

-

E

-

D
|
D

K
|
K

R
x
V

I
x
Y

L
x
V

F
x
G

G
x
T

A
x
L

G
x
D

N
x
G

W
x
R

L
|
L

M
x
I

A
|
A

V
x
L

D
|
D

A
|
A

L
x
K

T
|
T

G
|
G

N
x
K

P
x
A

I
|
I

S
x
W

E
x
S

F

-

G

-

D

-

G

-

K

-

V

-

D

-

L

-

R

-

K

-

N
x
Q

L
x
Q

D
x
T

T
|
T

D
|
D

R
x
P

D
x
A

D
x
K

F
x
P

L
x
Y

I
x
S

V
x
I

A
x
T

N
x
G

A
|
A

P
|
P

G
x
R

V
|
V

I
x
V

F

-

K
|
K

D
x
G

K
|
K

I
x
V

I
|
I

G
|
G

M
x
N

R
x
G

L
x
G

S
x
A

E
|
E

G

-

L

-

D
x
Y

A
x
G

A
x
V

P
x
R

G
|
G

H
x
F

I
x
V

R
x
S

A
|
A

Y
|
Y

N
x
D

V
x
A

K
x
D

T
|
T

G
|
G

K
|
K

R
x
L

E
x
A

W
|
W

I
x
R

F
|
F

H
x
Y

T
|
T

I
x
V

P
|
P

Q
x
G

K
x
D

G
x
P

E
x
A

F
x
L

G
x
P

Y
|
Y

D
x
E

-
x
H

-
x
P

-
x
E

-
x
L

-
x
R

-
x
E

-
x
A

-
x
K

T
|
T

W
|
W

D
x
Q

S
x
G

E
x
D

F
x
Q

I
x
Y

Q
x
W

K
|
K

I
x
L

G
|
G

A
x
G

N
x
G

N
x
T

-
x
V

W
|
W

A
x
D

G
x
S

M
|
M

V
x
A

V
x
Y

D
|
D

Q
x
P

K
x
E

R
x
L

E
x
D

I
x
L

V
x
L

F
x
Y

V
|
V

P
x
G

T
|
T

G
|
G

S
x
N

A
x
G

T
x
S

-
x
P

-
x
W

-
x
N

-
x
R

-
x
E

Y
x
V

D
x
R

F
x
S

W
x
P

G
|
G

Y

-

R

-

T

-

G
|
G

D
|
D

N
|
N

L
|
L

Y
|
Y

A
x
L

N
x
S

S
|
S

L
x
I

V
x
L

A
|
A

L
x
I

D
x
R

A
x
P

N
x
D

T
|
T

G
|
G

E
x
K

R
x
L

I
x
A

W
|
W

H
|
H

F
x
Y

Q
|
Q

A
x
V

V
x
T

H
x
P

H
x
G

D
|
D

V
x
S

W
|
W

D
|
D

R
x
F

D
x
T

F
x
A

P
x
T

A
x
Q

N
x
Q

P
x
I

N
x
T

L
|
L

I
x
A

R
x
E

I
x
L

Q
x
N

K
x
I

E
x
D

G
|
G

E
x
K

W
x
P

I
x
R

D
x
K

A
x
V

V
x
L

A
x
M

Q
|
Q

I
x
A

S
x
P

K
|
K

Q
x
N

G
|
G

M
x
F

T
x
F

Y
|
Y

V
|
V

F
x
L

D
|
D

R
|
R

E

-

S

-

G

-

E

-

P

-

I

-

W

-

P

-

I

-

V

-

E

-

T
|
T

N
|
N

V

-

P

-

Q

-

S

-

N

-

L

-

P

-

G
|
G

E
x
K

I
x
L

T
x
I

S
|
S

A
|
A

T

-

Q

-

P

-

I

-

P

-

T

-

L

-

P

-

E
|
E

P
x
K

F
|
F

M
x
G

N
x
K

M
x
V

T
|
T

F
x
W

-
x
A

E
|
E

D
x
K

S
x
V

D
|
D

I
x
L

L
x
A

S
x
T

L
x
G

K
x
R

P
|
P

E
x
V

W
x
E

E
x
A

E
x
P

D
x
G

I
x
V

R
|
R

S
x
Y

Q
x
E

L

-

V

-

N

-

V

-

Q

-

Y

-

G

-

D

-

T

-

W

-

A

-

P

-

S

-

E
x
K

H
x
E

G
x
P

I
|
I

I
x
V

L
x
M

F
x
W

P
|
P

G
x
S

M
x
P

D
x
F

G
|
G

G
x
A

G
x
H

E
x
N

W
|
W

G
x
H

G
x
S

A
x
M

S
|
S

F
|
F

D
x
N

P
|
P

S
x
G

N
x
T

Q
x
G

T
x
L

L
x
V

Y
|
Y

V
x
I

N
x
P

A
x
Y

N
x
Q

Q
x
E

I
x
V

P
|
P

W
x
G

L
x
V

I

-

E

-

M

-

T

-

P

-

N

-

A

-

E

-

F
x
Y

E
x
R

N
|
N

V
x
E

G
|
G

Q
x
K

S
x
D

I
x
F

Y
x
V

S
x
T

S
x
R

Y
x
K

C
x
A

G
x
F

N
|
N

-
x
T

C
x
A

H
x
A

G
|
G

L
x
F

S
x
A

R
x
D

K
x
A

G
x
T

D
|
D

L
x
V

P
|
P

A
|
A

E81 (≠ Q74) binding pyrroloquinoline quinone
C127 (≠ S118) modified: Disulfide link with 128
C128 (≠ W119) modified: Disulfide link with 127
R133 (= R124) binding pyrroloquinoline quinone
T177 (≠ A182) binding pyrroloquinoline quinone
GA 193:194 (≠ LS 199:200) binding pyrroloquinoline quinone
E195 (= E201) binding Ca(2+)
T252 (≠ N251) binding pyrroloquinoline quinone
N272 (≠ S270) binding Ca(2+)
D317 (= D313) binding Ca(2+)
K344 (= K340) binding pyrroloquinoline quinone
NW 404:405 (≠ EW 440:441) binding pyrroloquinoline quinone

Sites not aligning to the query:

1:22 signal peptide
23:690 modified: mature protein, Quinohemoprotein alcohol dehydrogenase ADH IIB
547 binding pyrroloquinoline quinone
613 binding covalent
616 binding covalent
617 binding axial binding residue
655 binding axial binding residue

8gy2A Cryo-em structure of membrane-bound alcohol dehydrogenase from gluconobacter oxydans
27% identity, 64% coverage: 17:465/705 of query aligns to 7:422/723 of 8gy2A

query
sites
8gy2A

A

A

C

I

T

T

S

S

E

S

E

D

N

N

N

G

I

G

D

H

Y

P

T

G

D

D

W

W

S

L

H

S

Y

Y

G

G

G

R

P

S

E

Y

D

S

G

E

S

Q

R

R

Y

Y

S

S

S

P

L

L

T

D

Q

Q

I

I

N

N

K

T

D

E

N

N

V

V

S

G

Q

K

L

L

A

K

Q

L

A

A

W

W

V

H

Y

Y

H

D

T

L

G

D

D

-

A

-

T

T

E

N

R

R

S

G

Q

Q

I

-

Q

E

C

G

Q

T

P

P

I

L

V

I

I

V

D

N

G

G

I

V

L

M

Y

Y

G

A

T

T

S

T

P

N

S

W

L

S

D

K

V

M

F

K

A

A

L

L

D

D

A

A

T

T

G

G

K

K

E

L

I

L

W

W

R

S

F

Y

D

D

P

P

F

K

-

V

-

P

D

G

I

N

L

I

G

A

G
x
D

E

R

N

G

S
x
C

W
x
C

A

D

G

T

T

V

N

S

R

R

G

G

V

A

S

A

Y

Y

W

W

A

-

E

-

A

-

E

-

D

N

K

G

R

K

I

V

L

Y

F

F

G

G

A

T

G

F

N

D

-

G

W

R

L

L

M

I

A

A

V

L

D

D

A

A

L

K

T

T

G

G

N

K

P

L

I

V

S

W

E

S

F

V

G

-

D

-

G

-

K

-

V

Y

D

T

L

I

R

P

K

K

N

E

L

A

D

Q

T

L

D

G

R

H

D

Q

D

R

F

S

L

Y

I

T

V

V

A
x
D

N

G

A

A

P

P

G

-

V

R

I

I

F

A

K

K

D

G

K

K

I

V

I

L

I

I

G

G

M

N

R

G

L
x
G

S
x
A

E
|
E

G

F

L

-

D

-

A

G

A

A

P

R

G

G

H

F

I

V

R

S

A

A

Y

F

N

D

V

A

K

E

T

T

G

G

K

K

R

L

E

D

W

W

I

R

F

F

H

F

T

T

I

V

P

P

Q

N

K

P

G

E

-

N

-

K

-

P

-

D

-

G

-

A

-

S

-

D

-

I

-

L

-

M

E

S

F

K

G

A

Y

Y

D

P

T

T

W

W

-

G

-

K

D

N

S

G

E

A

F

W

I

K

Q

Q

K

Q

I

G

G

G

A

G

N

T

N

V

W
|
W

A

D

G

S

M

L

V

V

V

Y

D

D

Q

P

K

V

R

T

E

D

I

L

V

V

F

Y

V

L

P

G

T

V

G

G

S
x
N

A

G

T

S

-

P

-

W

-

N

Y

Y

D

K

F

F

W

R

G

S

G

E

Y

G

R

K

T

-

G

G

D

D

N

N

L

L

Y

F

A

L

N

G

S

S

L

I

V

V

A

A

L

I

D

N

A

P

N

D

T

T

G

G

E

K

R

Y

I

V

W

W

H

H

F

F

Q

Q

A

E

V

T

H

P

H

M

D

D

V

E

W

W

D
|
D

R

Y

D

T

F

S

P

V

A

Q

N

Q

P

I

N

M

L

T

I

L

R

D

I

M

Q

P

K

V

E

N

G

G

E

E

W

M

I

R

D

H

A

V

V

I

A

V

Q

H

I

A

S

P

K
|
K

Q

N

G

G

M

F

T

F

Y

Y

V

I

F

I

D

D

R

A

E

K

S

T

G

G

E

K

P

F

I

I

W

-

P

-

I

-

V

-

E

-

T

-

N

-

V

-

P

-

Q

-

S

-

N

-

L

-

P

-

G

-

E

-

I

-

T

-

S

-

A

-

T

-

Q

-

P

-

I

-

P

-

T

-

L

T

P

G

E

K

P

P

F

Y

M

T

N

Y

M

E

T

N

F

W

E

A

D

N

S

G

-

L

D

D

I

P

L

V

S

T

L

G

K

R

P

P

E

N

W

Y

E

V

E

P

D

D

I

A

R

L

S

W

Q

T

L

L

V

T

N

-

V

-

Q

-

Y

-

G

G

D

K

T

P

W

W

A

-

P

-

S

-

E

-

H

-

G

-

I

-

I

L

L

G

F

I

P

P

G

G

M

E

D

L

G

G

G

H

E

N

W
x
F

G

A

A

M

S

A

F

Y

D

S

P

P

S

K

N

T

Q

K

T

L

L

V

Y

Y

V

I

N

P

A

A

N

Q

Q

Q

I

I

P

P

W

L

L

L

I

Y

E

D

binding calcium ion: E181 (= E201), N263 (≠ S270), D308 (= D313)
binding heme c: D104 (≠ G115)
binding pyrroloquinoline quinone: C107 (≠ S118), C108 (≠ W119), D163 (≠ A182), G179 (≠ L199), A180 (≠ S200), E181 (= E201), W245 (= W252), N263 (≠ S270), D308 (= D313), K335 (= K340), F398 (≠ W441)

Sites not aligning to the query:

binding heme c: 618, 619, 622, 623, 633, 634, 636, 639, 652, 660, 662, 665
binding pyrroloquinoline quinone: 489

1kv9A Structure at 1.9 a resolution of a quinohemoprotein alcohol dehydrogenase from pseudomonas putida hk5 (see paper)
27% identity, 67% coverage: 29:498/705 of query aligns to 17:433/664 of 1kv9A

query
sites
1kv9A

D

E

W

W

S

L

H

S

Y

H

G

G

G

R

P

T

E

Y

D

A

G

E

S

Q

R

R

Y

F

S

S

S

P

L

L

T

K

Q

Q

I

I

N

D

K

A

D

S

N

N

V

V

S

R

Q

S

L

L

A

G

Q

L

A

A

W

W

V

Y

Y

M

H

-

T

-

G

-

D

D

A

L

T

D

E

N

R

T

S

R

Q

G

I

L

Q
x
E

C

A

Q

T

P

P

I

L

V

F

I

H

D

D

G

G

I

V

L

I

Y

Y

G

T

T

S

S

M

P

S

S

W

L

S

D

R

V

V

F

I

A

A

L

V

D

D

A

A

T

S

G

G

K

K

E

E

I

L

W

W

R

R

F

Y

D

D

P

P

-

E

-

V

F

A

D

K

I

V

L

K

G
x
A

G
x
R

E

T

N

S

S
x
C

W
x
C

A

D

G

A

T

V

N

N

R
|
R

G

G

V

V

S

A

Y

L

W

W

A

G

E

-

A

-

E

-

D

D

K

K

R

V

I

Y

L

V

F

G

G

T

A

L

G

D

N

G

W

R

L

L

M

I

A

A

V

L

D

D

A

A

L

K

T

T

G

G

N

K

P

A

I

I

S

W

E

S

F

-

G

-

D

-

G

-

K

-

V

-

D

-

L

-

R

-

K

-

N

Q

L

Q

D

T

T

T

D

D

R

P

D

A

D

K

F

P

L

Y

I

S

V

I

A
x
T

N

G

A

A

P

P

G

R

V

V

I

V

F

-

K

K

D

G

K

K

I

V

I

I

G

G

M

N

R
x
G

L

G

S
x
A

E
|
E

G

-

L

-

D

Y

A

G

A

V

P

R

G

G

H

F

I

V

R

S

A

A

Y

Y

N

D

V

A

K

D

T

T

G

G

K

K

R

L

E

A

W

W

I

R

F

F

H

Y

T

T

I

V

P

P

Q

G

K

D

G

P

E

A

F

L

G

P

Y

Y

D

E

-

H

-

P

-

E

-

L

-

R

-

E

-

A

-

K

T

T

W

W

D

Q

S

G

E

D

F

Q

I

Y

Q

W

K

K

I

L

G

G

A

G

N

G

N
x
T

-

V

W
|
W

A

D

G

S

M

M

V

A

V

Y

D

D

Q

P

K

E

R

L

E

D

I

L

V

L

F

Y

V

V

P

G

T

T

G

G

S
x
N

A

G

T

S

Y

P

D

-

F

-

W

W

-

N

-

R

-

E

-

V

-

R

-

S

-

P

G

G

Y

-

R

-

T

-

G

G

D

D

N

N

L

L

Y

Y

A

L

N

S

S

S

L

I

V

L

A

A

L

I

D

R

A

P

N

D

T

T

G

G

E

K

R

L

I

A

W

W

H

H

F

Y

Q

Q

A

V

V

T

H

P

H

G

D

D

V

S

W

W

D
|
D

R

F

D

T

F

A

P

T

A

Q

N

Q

P

I

N

T

L

L

I

A

R

E

I

L

Q

N

K

I

E

D

G

G

E

K

W

P

I

R

D

K

A

V

V

L

A

M

Q

Q

I

A

S

P

K
|
K

Q

N

G

G

M

F

T

F

Y

Y

V

V

F

L

D

D

R

R

E

-

S

-

G

-

E

-

P

-

I

-

W

-

P

-

I

-

V

-

E

-

T

T

N

N

V

-

P

-

Q

-

S

-

N

-

L

-

P

-

G

G

E

K

I

L

T

I

S

S

A

A

T

-

Q

-

P

-

I

-

P

-

T

-

L

-

P

-

E

E

P

K

F

F

M

G

N

K

M

V

T

T

F

W

-

A

E

E

D

K

S

V

D

D

I

L

L

A

S

T

L

G

K

R

P

P

E

V

W

E

E

A

E

P

D

G

I

V

R

R

S

Y

Q

E

L

-

V

-

N

-

V

-

Q

-

Y

-

G

-

D

-

T

-

W

-

A

-

P

-

S

-

E

K

H

E

G

P

I

I

I

V

L

M

F

W

P

P

G

S

M

P

D

F

G

G

G

A

G

H

E
x
N

W
|
W

G

H

G

S

A

M

S

S

F

F

D

N

P

P

S

G

N

T

Q

G

T

L

L

V

Y

Y

V

I

N

P

A

Y

N

Q

Q

E

I

V

P

P

W

G

L

V

I

-

E

-

M

-

T

-

P

-

N

-

A

-

E

-

F

Y

E

R

N

N

V

E

G

G

Q

K

S

D

I

F

Y

V

S

T

S

R

Y

K

C

A

G

F

N

N

-

T

C

A

H

A

G

G

L

F

S

A

R

D

K

A

G

T

D

D

L

V

P

P

A

A

active site: E173 (= E201), N250 (≠ S270), D295 (= D313)
binding acetone: E173 (= E201), D295 (= D313)
binding calcium ion: E173 (= E201), N250 (≠ S270), D295 (= D313)
binding heme c: A101 (≠ G114), R102 (≠ G115)
binding pyrroloquinoline quinone: E59 (≠ Q74), C105 (≠ S118), C106 (≠ W119), R111 (= R124), T155 (≠ A182), G170 (≠ R198), A172 (≠ S200), E173 (= E201), T230 (≠ N251), W232 (= W252), K322 (= K340), N382 (≠ E440), W383 (= W441)

Sites not aligning to the query:

binding heme c: 590, 591, 594, 595, 605, 606, 608, 611, 615, 619, 623, 631, 633, 636
binding pyrroloquinoline quinone: 460, 525

Q46444 Quinohemoprotein alcohol dehydrogenase; QH-ADH; Alcohol dehydrogenase (azurin); PQQ-containing alcohol dehydrogenase; PQQ-dependent ADH; Quinohaemoprotein ethanol dehydrogenase type I; QH-EDHI; EC 1.1.9.1 from Comamonas testosteroni (Pseudomonas testosteroni) (see 3 papers)
31% identity, 46% coverage: 29:355/705 of query aligns to 59:381/708 of Q46444

query
sites
Q46444

D

D

W

W

S

P

H

T

Y

I

G

G

G

V

P

D

E

Y

D

A

G

E

S

T

R

R

Y

Y

S

S

S

R

L

L

T

D

Q

Q

I

I

N

N

K

A

D

A

N

N

V

V

S

K

Q

D

L

L

A

G

Q

L

A

A

W

W

V

S

Y

Y

H

-

T

-

G

-

D

N

A

L

T

E

E

S

R

T

S

R

Q

G

I

V

Q
x
E

C

A

Q

T

P

P

I

V

V

V

I

V

D

D

G

G

I

I

L

M

Y

Y

G

V

T

S

S

A

P

S

S

W

L

S

D

V

V

V

F

H

A

A

L

I

D

D

A

T

A

R

T

T

G

G

K

N

E

R

I

I

W

W

R

T

F

Y

D

D

P

P

F

Q

-

I

-

D

D

R

I

S

L

T

G

G

G

F

E

K

N

G

S
x
C

W
x
C

A

D

G

V

T

V

N

N

R
|
R

G

G

V

V

S

A

Y

L

W

W

A

-

E

-

A

-

E

-

D

K

K

G

R

K

I

V

L

Y

F

V

G

G

A

A

G

-

N

-

W

W

-

D

-

G

-

R

L

L

M

I

A

A

V

L

D

D

A

A

L

A

T

T

G

G

N

K

P

E

I

V

S

-

E

-

F

-

G

-

D

-

G

-

K

-

V

-

D

-

L

W

R

H

K

Q

N

N

L

T

D

F

T

E

D

G

R

Q

D

K

D

G

F

S

L

L

I

T

V

I

A
x
T

N

G

A

A

P

P

G

R

V

V

I

-

F

F

K

K

D

G

K

K

I

V

I

I

G

G

M

K

R

R

L
x
G

S
x
A

E
|
E

G

-

L

-

D

Y

A

G

A

V

P

R

G

G

H

Y

I

I

R

T

A

A

Y

Y

N

D

V

A

K

E

T

T

G

G

K

E

R

R

E

K

W

W

I

R

F

W

H

F

T

S

I

V

P

P

-

G

-

D

-

P

-

S

-

K

-

P

-

F

-

E

-

D

Q

E

K

S

G

M

E

K

F

R

G

A

Y

A

D

R

T

T

W

W

D

D

-

P

-

S

S

G

E

K

F

W

I

W

Q

E

K

A

I

G

G

G

A

G

N
x
T

N

M

W

W

A

D

G

S

M

M

V

T

V

F

D

D

Q

A

K

E

R

L

E

N

I

T

V

M

F

Y

V

V

P

G

T

T

G

G

S
x
N

A

G

T

S

Y

P

D

-

F

-

W

W

G

S

-

H

-

K

-

V

-

R

G

S

Y

P

R

K

T

G

G

G

D

D

N

N

L

L

Y

Y

A

L

N

A

S

S

L

I

V

V

A

A

L

L

D

D

A

P

N

D

T

T

G

G

E

K

R

Y

I

K

W

W

H

H

F

Y

Q

Q

A

E

V

T

H

P

H

G

D

D

V

N

W

W

D
|
D

R

Y

D

T

F

S

P

T

A

Q

N

P

P

M

N

I

L

L

I

A

R

D

I

I

Q

K

K

I

E

A

G

G

E

K

W

P

I

R

D

K

A

V

V

I

A

L

Q

H

I

A

S

P

K
|
K

Q

N

G

G

M

F

T

F

Y

F

V

V

F

L

D

D

R

R

E

T

S

N

G

G

E

K

P

F

I

I

E101 (≠ Q74) binding pyrroloquinoline quinone
C147 (≠ S118) modified: Disulfide link with 148
C148 (≠ W119) modified: Disulfide link with 147
R153 (= R124) binding pyrroloquinoline quinone
T198 (≠ A182) binding pyrroloquinoline quinone
GA 214:215 (≠ LS 199:200) binding pyrroloquinoline quinone
E216 (= E201) binding Ca(2+)
T274 (≠ N250) binding pyrroloquinoline quinone
N294 (≠ S270) binding Ca(2+)
D339 (= D313) binding Ca(2+)
K366 (= K340) binding pyrroloquinoline quinone

Sites not aligning to the query:

1:31 signal peptide
425:426 binding pyrroloquinoline quinone
575 binding pyrroloquinoline quinone
635 binding covalent
638 binding covalent
639 binding axial binding residue
678 binding axial binding residue

6zcvA Crystal structure of lanthanide-dependent alcohol dehydrogenase pedh from pseudomonas putida kt2440
31% identity, 46% coverage: 39:360/705 of query aligns to 26:345/562 of 6zcvA

query
sites
6zcvA

G

G

S

Q

R

R

Y

Y

S

S

S

P

L

L

T

D

Q

L

I

L

N

N

K

V

D

N

N

N

V

V

S

K

Q

E

L

L

A

R

Q

P

A

V

W

W

V

A

Y

F

H

S

T

F

G

G

D

G

A

E

T

K

E

Q

R

R

S

G

Q

Q

I

-

Q
|
Q

C

A

Q

Q

P

P

I

L

V

I

I

K

D

D

G

G

I

V

L

M

Y

Y

G

L

T

T

S

G

P

S

S

Y

L

S

D

R

V

V

F

F

A

A

L

V

D

D

A

A

A

R

T

T

G

G

K

K

E

K

I

L

W

W

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