SitesBLAST
Comparing WP_008410843.1 NCBI__GCF_000315365.1:WP_008410843.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3d31A Modbc from methanosarcina acetivorans (see paper)
35% identity, 84% coverage: 25:330/363 of query aligns to 16:321/348 of 3d31A
Sites not aligning to the query:
P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
32% identity, 82% coverage: 19:316/363 of query aligns to 29:333/378 of P69874
- F45 (≠ T35) mutation to L: Lower ATPase activity and transport efficiency.
- C54 (≠ A44) mutation to T: Loss of ATPase activity and transport.
- L60 (≠ V50) mutation to F: Lower ATPase activity and transport efficiency.
- L76 (≠ F66) mutation to P: Lower ATPase activity and transport efficiency.
- V135 (≠ L125) mutation to M: Loss of ATPase activity and transport.
- D172 (= D162) mutation to N: Loss of ATPase activity and transport.
- C276 (vs. gap) mutation to A: Lower ATPase activity and transport efficiency.
- E297 (= E283) mutation E->K,D: Lower ATPase activity and transport efficiency.; mutation to Q: Loss of ATPase activity and transport.
Sites not aligning to the query:
- 26 C→A: Lower ATPase activity and transport efficiency.
- 27 F→L: Lower ATPase activity and transport efficiency.
P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
30% identity, 96% coverage: 6:354/363 of query aligns to 4:353/371 of P68187
- A85 (= A89) mutation to M: Suppressor of EAA loop mutations in MalFG.
- K106 (≠ P110) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V114 (= V118) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V117 (≠ W121) mutation to M: Suppressor of EAA loop mutations in MalFG.
- E119 (≠ K123) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- A124 (= A128) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G137 (= G141) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
- D158 (= D162) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
- R228 (≠ E232) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- F241 (vs. gap) mutation to I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- W267 (≠ G258) mutation to G: Normal maltose transport but constitutive mal gene expression.
- G278 (= G273) mutation to P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- S282 (≠ N277) mutation to L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G284 (≠ C279) mutation to S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G302 (= G302) mutation to D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- E308 (≠ Y308) mutation to Q: Maltose transport is affected but retains ability to interact with MalT.
- S322 (≠ Q327) mutation to F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G340 (= G341) mutation to A: Maltose transport is affected but retains ability to interact with MalT.
- G346 (≠ M347) mutation to S: Normal maltose transport but constitutive mal gene expression.
Sites not aligning to the query:
- 355 F→Y: Maltose transport is affected but retains ability to interact with MalT.
1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
30% identity, 96% coverage: 6:354/363 of query aligns to 1:350/367 of 1q12A
- binding adenosine-5'-triphosphate: W10 (= W15), S35 (≠ N42), G36 (= G43), C37 (≠ A44), G38 (= G45), K39 (= K46), S40 (= S47), T41 (≠ S48), R126 (= R133), A130 (≠ Y137), S132 (= S139), G134 (= G141), Q135 (≠ E142)
2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
30% identity, 96% coverage: 6:354/363 of query aligns to 3:352/374 of 2awnB
3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
30% identity, 96% coverage: 6:354/363 of query aligns to 3:352/371 of 3puyA
- binding phosphoaminophosphonic acid-adenylate ester: W12 (= W15), S37 (≠ N42), G38 (= G43), C39 (≠ A44), G40 (= G45), K41 (= K46), S42 (= S47), T43 (≠ S48), Q81 (= Q87), R128 (= R133), A132 (≠ Y137), S134 (= S139), G136 (= G141), Q137 (≠ E142), E158 (= E163), H191 (= H196)
- binding magnesium ion: S42 (= S47), Q81 (= Q87)
3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
30% identity, 96% coverage: 6:354/363 of query aligns to 3:352/371 of 3puxA
- binding adenosine-5'-diphosphate: W12 (= W15), G38 (= G43), C39 (≠ A44), G40 (= G45), K41 (= K46), S42 (= S47), T43 (≠ S48), R128 (= R133), S134 (= S139), Q137 (≠ E142)
- binding beryllium trifluoride ion: S37 (≠ N42), G38 (= G43), K41 (= K46), Q81 (= Q87), S134 (= S139), G136 (= G141), H191 (= H196)
- binding magnesium ion: S42 (= S47), Q81 (= Q87)
3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
30% identity, 96% coverage: 6:354/363 of query aligns to 3:352/371 of 3puwA
- binding adenosine-5'-diphosphate: W12 (= W15), V17 (≠ I23), G38 (= G43), C39 (≠ A44), G40 (= G45), K41 (= K46), S42 (= S47), T43 (≠ S48), R128 (= R133), A132 (≠ Y137), S134 (= S139), Q137 (≠ E142)
- binding tetrafluoroaluminate ion: S37 (≠ N42), G38 (= G43), K41 (= K46), Q81 (= Q87), S134 (= S139), G135 (= G140), G136 (= G141), E158 (= E163), H191 (= H196)
- binding magnesium ion: S42 (= S47), Q81 (= Q87)
3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
30% identity, 96% coverage: 6:354/363 of query aligns to 3:352/371 of 3puvA
- binding adenosine-5'-diphosphate: W12 (= W15), V17 (≠ I23), G38 (= G43), C39 (≠ A44), G40 (= G45), K41 (= K46), S42 (= S47), T43 (≠ S48), R128 (= R133), A132 (≠ Y137), S134 (= S139), Q137 (≠ E142)
- binding magnesium ion: S42 (= S47), Q81 (= Q87)
8y5iA Cryo-em structure of e.Coli spermidine transporter potd-potabc in translocation intermidiate state (see paper)
32% identity, 82% coverage: 19:316/363 of query aligns to 14:318/358 of 8y5iA
Sites not aligning to the query:
1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
32% identity, 77% coverage: 4:283/363 of query aligns to 3:276/353 of 1vciA
P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
36% identity, 58% coverage: 20:229/363 of query aligns to 16:232/343 of P30750
- 40:46 (vs. 42:48, 71% identical) binding ATP
- E166 (= E163) mutation to Q: Exhibits little ATPase activity.
Sites not aligning to the query:
- 278:283 binding L-methionine
- 295 N→A: Reduces the binding of L-methionine to undetectable levels.
- 295:296 binding L-methionine
6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
36% identity, 58% coverage: 20:229/363 of query aligns to 17:233/344 of 6cvlD
- binding phosphothiophosphoric acid-adenylate ester: I19 (≠ L22), S41 (≠ N42), G42 (= G43), A43 (= A44), G44 (= G45), K45 (= K46), S46 (= S47), T47 (≠ S48), N141 (vs. gap), S143 (= S139), Q146 (≠ E142), H200 (= H196)
Sites not aligning to the query:
3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
36% identity, 58% coverage: 20:229/363 of query aligns to 17:233/344 of 3tuzC
Sites not aligning to the query:
3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
36% identity, 58% coverage: 20:229/363 of query aligns to 17:233/344 of 3tuiC