SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_008412468.1 NCBI__GCF_000315365.1:WP_008412468.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P09099 Xylulose kinase; XK; Xylulokinase; 1-deoxy-D-xylulokinase; EC 2.7.1.17; EC 2.7.1.- from Escherichia coli (strain K12) (see paper)
40% identity, 98% coverage: 4:503/510 of query aligns to 1:483/484 of P09099

query
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P09099

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D6 (= D9) mutation to A: Loss of activity.
MH 77:78 (= MH 81:82) binding substrate
D233 (= D240) mutation to A: Loss of activity.

2itmA Crystal structure of the e. Coli xylulose kinase complexed with xylulose (see paper)
39% identity, 98% coverage: 4:503/510 of query aligns to 1:475/476 of 2itmA

query
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binding d-xylulose: M77 (= M81), H78 (= H82), W96 (= W100), D233 (= D240), N234 (= N241), M288 (= M295)

3ll3B The crystal structure of ligand bound xylulose kinase from lactobacillus acidophilus
27% identity, 98% coverage: 6:507/510 of query aligns to 5:490/490 of 3ll3B

query
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3ll3B

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D

G

K

Q

T

W

H

H

Y

L

L

M

L

G

G

V

G

T

P

M

V

A
x
N

A

N

G

G

G
|
G

-

I

S

V

L

F

R

N

W

W

Y

A

R

R

D

Q

T

T

F

L

A

F

G

A

E

D

E

E

R

T

A

-

V

-

A

-

Q

-

L

-

T

-

G

-

R

-

D

-

P
|
P

Y

Q

E

D

L

F

M

L

S

-

D

D

E

V

A

A

A

Q

L

T

I

A

E

P

P

A

G

G

A

S

D

R

G

N

L

L

I

I

F

F

L

L

P

P

Y

Y

L

L

S
x
G

G

G

E
|
E

R

R

T

A

P

P

H

I

A

W

D

D

P
x
A

L

N

A

A

R

R

G

G

V

S

F

F

V

V

G

G

M

L

N

T

L

R

K

M

H

H

T

Q

K

K

G

P

H

E

F

M

V

A

R

R

S

A

I

V

M

I

E

E

G

G

V

I

A

I

F

F

S

N

L

L

K

Y

D

D

-

A

-

A

-

S

T

N

L

L

E

I

I

K

M

N

S

T

G

K

L

K

G

P

I

V

A

A

L

I

N

N

D

-

L

-

R

-

I

A

T

T

G

G

G
|
G

G
x
F

G

L

R
x
K

S

S

A

D

V

F

W

V

R

R

Q

Q

I

L

L

C

A

A

D

N

V

I

F

F

N

N

M

V

P

P

L

I

Q

V

V

T

M

M

E

K

N

E

S

Q

D

Q

G

S

P

G

A

T

Y

L

G

A

A

A

A

M

I

F

L

L

G

A

A

R

V

Q

G

A

A

L

G

G

C

L

W

N

Q

Q

S

D

V

L

T

S

E

E

A

I

V

G

A

Q

A

F

T

A

C

-

S

-

R

-

Q

Q

Q

A

L

D

Q

K

E

V

V

Y

V

F

P

P

I

N

P

P

Q

K

N

E

A

A

A

A

R

T

Y

Y

Q

Q

K

K

I

L

Y

F

Q

P

I

L

Y

Y

I

C

D

E

T

I

Y

R

P

N

P

A

L

L

Q

A

P

A

L

S

F

Y

K

G

R

K

L

F

S

S

binding adenosine-5'-diphosphate: G258 (= G261), T259 (= T262), G298 (= G303), P314 (= P327), G399 (= G413), F400 (≠ G414), K402 (≠ R416)
binding 1-deoxy-d-xylulose-5-phosphate: H127 (≠ L130), N295 (≠ A300), G338 (≠ S352), E340 (= E354), A347 (≠ P361)

3ll3A The crystal structure of ligand bound xylulose kinase from lactobacillus acidophilus
28% identity, 98% coverage: 6:507/510 of query aligns to 6:492/492 of 3ll3A

query
sites
3ll3A

L

I

G

G

I

M

D

D

I

V

G

G

T

T

T

T

G

A

V

T

K

K

A

G

L

V

V

L

M

Y

D

D

E

I

Q

N

G

G

R

K

V

A

V

V

A

A

Q

S

A

V

T

S

R

K

E

G

Y

Y

P

P

L

L

H

I

V

Q

P

T

R

K

P

V

G

G

W

Q

A

A

E

E

Q

E

N

D

P

P

E

K

D

L

W

I

Y

F

K

D

A

A

A

V

C

Q

G

E

A

I

I

I

A

F

T

D

I

L

I

T

S

Q

K

K

G

-

I

-

S

I

P

D

G

G

Q

K

I

I

Q

A

G

A

I

I

G

S

L

W

T

S

G

S

Q
|
Q

M
|
M

H
|
H

G

S

S

L

V

I

F

G

L

L

D

G

A

S

Q

D

G

D

K

E

V

L

I

L

R

T

E

N

A

S

I

I

L

T

W

W

C

A

D

D

Q

N

R

C

T

A

A

K

D

S

E

I

C

V

R

Q

Q

D

I

A

T

K

D

N

L

R

V

G

G

F

E

A

S

Q

K

Q

L

I

I

Y

E

R

L

K

V

T

S

G

N

M

P

P

A

M

L
x
H

A

P

G

M

F

A

T

P

A

I

P

Y

K

K

I

L

L

L

W

W

L

L

R

K

N

N

H

K

E

K

P

T

E

E

N

V

Y

F

K

S

R

Q

V

A

A

Q

K

K

V

W

L

I

L

G

P

I

K

K

D

E

Y

Y

I

I

S

I

W

F

R

R

M

L

T

T

G

G

V

K

F

L

A

V

T

T

D

D

V

T

S

T

D

M

A

A

S

A

G

G

M

T

L

G

L

I

L

L

D

N

V

L

V

K

N

T

R

L

K

T

W

W

S

D

S

Q

E

E

M

L

L

L

A

D

A

I

L

L

E

K

I

I

P

K

A

K

A

E

W

Q

L

L

A

P

E

K

V

I

F

A

E

Q

S

P

P

T

D

K

V

V

V

I

G

F

R

P

V

I

S

K

A

T

R

E

G

Y

A

V

A

K

E

K

T

L

G

G

L

I

P

D

E

S

G

D

I

T

P

K

V

I

V

I

A

L

G

G

A

A

G

S

D
|
D

N

G

A

Y

A

L

G

S

A

T

V

I

G

G

N

V

G

N

I

A

I

I

S

D

P

S

G

D

M

H

A

C

T

A

A

L

S

N

L

V

G
|
G

T
|
T

S

S

G

G

V

A

V

I

F

R

T

T

P

I

S

V

Q

D

T

Q

P

P

A

K

V

I

D

D

R

P

A

S

G

A

R

S

L

Y

H

-

T

-

F

F

C

C

H

Y

A

P

V

A

P

D

G

K

Q

T

W

H

H

Y

L

L

M

L

G

G

V

G

T

P

M

V

A
x
N

A

N

G

G

G
|
G

-

I

S

V

L

F

R

N

W

W

Y

A

R

R

D

Q

T

T

-

L

F

F

A

D

G

A

E

D

E

E

R

T

A

-

V

-

A

-

Q

-

L

-

T

-

G

-

R

-

D

-

P
|
P

Y

Q

E

D

L

F

M
x
L

S

-

D

D

E

V

A

A

A

Q

L

T

I

A

E

P

P

A

G

G

A

S

D

R

G

N

L

L

I

I

F

F

L

L

P

P

Y

Y

L

L

S

G

G

G

E
|
E

R
|
R

T

A

P

P

H

I

A

W

D

D

P
x
A

L

N

A

A

R

R

G

G

V

S

F

F

V

V

G

G

M

L

N

T

L

R

K

M

H

H

T

Q

K

K

G

P

H

E

F

M

V

A

R

R

S

A

I

V

M

I

E

E

G

G

V

I

A

I

F

F

S

N

L

L

K

Y

D

D

-

A

-

A

-

S

T

N

L

L

E

I

I

K

M

N

S

T

G

K

L

K

G

P

I

V

A

A

L

I

N

N

D

-

L

-

R

-

I

A

T

T

G
|
G

G
|
G

G
x
F

G

L

R

K

S

S

A

D

V

F

W

V

R

R

Q

Q

I

L

L

C

A

A

D

N

V

I

F

F

N

N

M

V

P

P

L

I

Q

V

V

T

M

M

E

K

N

E

S

Q

D

Q

G

S

P

G

A

T

Y

L

G

A

A

A

A

M

I

F

L

L

G

A

A

R

V

Q

G

A

A

L

G

G

C

L

W

N

Q

Q

S

D

V

L

T

S

E

E

A

I

V

G

A

Q

A

F

T

A

C

-

S

-

R

-

Q

Q

Q

A

L

D

Q

K

E

V

V

Y

V

F

P

P

I

N

P

P

Q

K

N

E

A

A

A

A

R

T

Y

Y

Q

Q

K

K

I

L

Y

F

Q

P

I

L

Y

Y

I

C

D

E

T

I

Y

R

P

N

P

A

L

L

Q

A

P

A

L

S

F

Y

K

G

R

K

L

F

S

S

binding adenosine-5'-triphosphate: G259 (= G261), T260 (= T262), G299 (= G303), P316 (= P327), L320 (≠ M331), G400 (= G412), G401 (= G413), F402 (≠ G414)
binding 1-deoxy-d-xylulose-5-phosphate: H128 (≠ L130), N296 (≠ A300), E342 (= E354), A349 (≠ P361)
binding d-xylulose: Q78 (= Q80), M79 (= M81), H80 (= H82), D238 (= D240), R343 (= R355)

P18157 Glycerol kinase; ATP:glycerol 3-phosphotransferase; Glycerokinase; GK; EC 2.7.1.30 from Bacillus subtilis (strain 168) (see paper)
29% identity, 90% coverage: 1:461/510 of query aligns to 1:458/496 of P18157

query
sites
P18157

M

M

Q

E

S

T

L

Y

F

I

L

L

G

S

I

L

D

D

I

Q

G

G

T

T

T

T

G

S

V

S

K

R

A

A

L

I

V

L

M

F

D

N

E

K

Q

E

G

G

R

K

V

I

V

V

A

H

Q

S

A

A

T

Q

R

K

E

E

Y

F

P

T

L

Q

H

Y

V

F

P

P

R

H

P

P

G

G

W

W

A

V

E

E

Q

H

N

N

P

A

E

N

D

E

W

I

Y

W

K

G

A

S

A

V

C

L

G

A

A

V

I

I

A

A

T

S

I

V

I

I

S

S

K

E

-

S

G

G

I

I

S

S

P

A

G

S

Q

Q

I

I

Q

A

G

G

I

I

G

G

L

I

T

T

G

N

Q

Q

M

R

H

E

G

T

S

T

V

V

F

V

L

W

D

D

A

K

Q

D

-

T

G

G

K

S

V

P

I

V

R

Y

E

N

A

A

I

I

L

V

W

W

C

Q

D

S

Q

R

R

Q

T

T

A

S

D

G

E

I

C

C

R

E

Q

E

I

L

T

R

D

E

L

K

V

G

G

Y

E

N

S

D

K

K

L

F

I

R

E

E

-

K

-

T

-

G

L

L

V

L

S

I

N

D

P

P

A

Y

L

-

A

-

G

-

F

F

T

S

A

G

P

T

K

K

I

V

L

K

W

W

-

I

L

L

R

D

N

N

H

V

E

E

P

G

E

A

N

R

Y

E

K

K

R

A

V

E

A

K

K

G

V

E

L

L

L

L

-

F

-

G

-

T

P

I

K

D

D

T

Y

W

I

L

S

I

W

W

R

K

M

M

T

S

G

G

-

G

-

K

V

A

F

H

A

V

T

T

D

D

V

Y

S

S

D

N

A

A

S

S

G

R

M

T

L

L

L

M

L

F

D

N

V

I

V

Y

N

D

R

L

K

K

W

W

S

D

S

D

E

E

M

L

L

L

A

D

A

I

L

L

E

G

I

V

P

P

A

K

A

S

W

M

L

L

A

P

E

E

V

V

F

K

E

P

S

S

P

S

D

-

V

-

V

-

G

-

R

H

V

V

S

Y

A

A

R

E

G

T

A

V

A

D

E

Y

T
x
H

G

F

L
x
F

P

G

E

K

G

N

I

I

P

P

V

I

V

A

A

G

G

A

A

A

G

G

D

D

N

Q

A

Q

A

S

G

A

A

L

V

F

G

G

N

Q

G

A

I

C

I

F

S

E

P

E

G

G

M

M

A

G

T

K

A

N

S

T

L

Y

G

G

T

T

S

G

G

C

V

F

V

M

F

L

T

M

P

N

S

T

Q

G

T

E

P

K

A

A

V

I

-

K

D

S

R

E

A

H

G

G

R

L

L

L

H

T

T

T

F

I

C

A

H

W

A

G

V

I

P

D

G

G

Q

K

-

V

-

N

W

Y

H

A

L

L

M

E

G

G

V

S

T

I

M

F

A

V

A

A

G

G

G

S

S

A

L

I

R

Q

W

W

Y

L

R

R

D

D

T

G

F

L

A

-

G

-

E

-

E

-

R

R

A

M

V

F

A

Q

Q

D

L

S

T

S

G

L

R

S

D

E

P

S

Y

Y

E

A

L

E

M

K

S

V

D

D

E

-

A

-

A

-

L

-

I

-

E

-

P

-

G

S

A

T

D

D

G

G

L

V

I

Y

F

V

L

V

P

P

Y

A

L

F

S

V

G

G

E

L

R

G

T

T

P

P

H

Y

A

W

D

D

P

S

L

D

A

V

R

R

G

G

V

S

F

V

V

F

G

G

M

L

N

T

L

R

K

G

H

T

T

T

K

K

G

E

H

H

F

F

V

I

R

R

S

A

I

T

M

L

E

E

G

S

V

L

A

A

F

Y

S

Q

L

T

K

K

D

D

T

V

L

L

E

D

I

A

M

M

S

E

G

A

-

D

L

S

G

N

I

I

A

S

L

L

N

K

D

T

L

L

R

R

I

V

T

D

G

G

G

G

G

A

G

V

R

K

S

N

A

N

V

F

W

L

R

M

Q

Q

I

F

L

Q

A

G

D

D

V

L

F

L

N

N

M

V

P

P

L

V

Q

E

V

R

M

P

E

E

N

I

S

N

D

E

G

T

P

T

A

A

Y

L

G

G

A

A

A

A

I

Y

L

L

G

A

A

G

V

I

G

A

A

V

G

G

C

F

W

W

Q

K

S

D

V

R

T

S

E

E

H230 (≠ T226) mutation to R: Increased activity.
F232 (≠ L228) mutation to S: Increased activity.

6k76A Glycerol kinase form thermococcus kodakarensis, complex structure with substrate.
28% identity, 89% coverage: 6:461/510 of query aligns to 2:443/485 of 6k76A

query
sites
6k76A

L

L

G

S

I

L

D

D

I

E

G

G

T
|
T

T

T

G

S

V

A

K

R

A

A

L

I

V

I

M

F

D

D

E

R

Q

E

G

S

R

N

V

I

V

H

A

G

Q

I

A

G

T

Q

R

Y

E

E

Y

F

P

P

L

Q

H

H

V

Y

P

P

R

R

P

P

G

G

W

W

A

V

E

E

Q

H

N

N

P

P

E

E

D

E

W

I

Y

W

K

D

A

A

A

Q

C

L

G

R

A

A

I

I

A

K

T

D

I

A

I

I

S

Q

K

S

G

A

I

R

S

E

P

P

G

N

Q

Q

I

I

Q

A

G

A

I

I

G

G

L

V

T

T

G

N

Q

Q

M

R

H

E

G

T

S

T

V

L

F

V

L

W

D

D

A

K

Q

D

G

G

K

K

V

P

I

L

R

Y

E

N

A

A

I

I

L

V

W

W

C

Q

D

C

Q

R

R

R

T

T

A

A

D

E

E

M

C

V

R

E

Q

E

I

I

T

K

D

R

L

E

V

Y

G

G

E

-

S

T

K

M

L

I

I

K

E

E

L

K

V

T

S

G

N

L

P

V

A

P

L

D

A

A

G

Y

F

F

T

S

A

A

P

S

K

K

I

L

L

K

W

W

L

L

R

L

N

D

H

N

E

V

P

P

E

G

N

L

Y

R

K

E

R

K

V

A

A

E

K

K

-

G

-

E

V

V

L

M

L

F

-

G

-

T

P

V

K

D

D

T

Y

F

I

L

S

I

W

Y

R

R

M

L

T

T

G

G

V

E

F

H

A

V

T

T

D

D

V

Y

S

S

D

N

A

A

S

S

G

R

M

T

L

M

L

L

L

F

D

N

V

I

V

K

N

K

R

L

K

D

W

W

S

D

S

D

E

E

M

L

L

L

A

E

A

L

L

F

E

D

I

I

P

P

A

E

A

S

W

V

L

L

A

P

E

E

V

V

F

R

E

E

S

S

P

S

D

E

V

V

V

Y

G

G

R

Y

V

T

S

K

A

K

R

E

G

L

A

A

A

E

E

-

T

-

G

-

L

-

P

-

E

-

G

-

I

I

P

P

V

V

V

S

A

G

G

D

A

A

G

G

D

D

N

Q

A

Q

A

A

G

A

A

L

V

F

G

G

N

Q

G

A

I

A

I

F

S

E

P

A

G

G

M

M

A

V

T

K

A

A

S

T

L

Y

G

G

T
|
T

S

G

G

S

V

F

V

I

F

L

T

V

P

N

S

T

Q

D

T

K

P

M

A

V

V

L

D

Y

R

S

A

D

G

N

R

L

L

L

H

T

T

T

F

I

C

A

H

W

A

G

V

L

P

N

G

G

Q

R

-

V

-

S

W

Y

H

A

L

L

M

E

G

G

V

S

T

I

M

F

A

V

A

T

G
|
G

G

A

S

A

L
x
V

R
x
Q

W

W

Y

L

R

R

D

D

T

G

F

I

A

K

G

I

E

I

E

K

R

H

A

A

V

S

A

E

Q

-

L

-

T

-

G

-

R

-

D

-

P

-

Y

-

E

-

L

-

M

-

S

T

D

E

E

E

A

L

A

A

L

T

I

K

E

L

P

E

G

S

A

N

D

E

G

G

L

V

I

Y

F

F

L

V

P

P

Y

A

L

F

S

V

G

G

E

L

R

G

T

A

P

P

H

Y

A

W

D

D

P

Q

L

F

A

A

R

R

G

G

V

I

F

I

V

I

G

G

M

I

N

T

L

R

K

G

H

T

T

G

K

R

G

E

H

H

F

L

V

A

R

R

S

A

I

T

M

L

E

E

G

A

V

I

A

A

F

Y

S

L

L

T

K

R

D

D

T

V

L

V

E

D

I

E

M

M

S

E

G

K

L

L

G

-

I

V

A

Q

L

I

N

K

D

E

L

L

R

R

I

V

T

D

G

G

G
|
G

G
x
A

G

T

R

A

S
x
N

A

D

V

F

W

L

R

M

Q

Q

I

F

L

Q

A

A

D

D

V

I

F

L

N

N

M

R

P

K

L

V

Q

I

V

R

M

P

E

V

N

V

S

K

D

E

G

T

P

T

A

A

Y

L

G

G

A

A

A

A

I

Y

L

L

G

A

A

G

V

L

G

A

A

V

G

D

C

Y

W

W

Q

A

S

D

V

T

T

R

E

E

binding phosphoaminophosphonic acid-adenylate ester: T8 (= T12), T254 (= T262), G296 (= G302), V299 (≠ L305), Q300 (≠ R306), G395 (= G413), A396 (≠ G414), N399 (≠ S417)

1bu6Y Crystal structures of escherichia coli glycerol kinase and the mutant a65t in an inactive tetramer: conformational changes and implications for allosteric regulation (see paper)
27% identity, 95% coverage: 2:485/510 of query aligns to 2:482/499 of 1bu6Y

query
sites
1bu6Y

Q

K

S

K

L

Y

F

I

L

V

G

A

I

L

D

D

I

Q

G

G

T

T

T

T

G

S

V

S

K

R

A

A

L

V

V

V

M

M

D

D

E

H

Q

D

G

A

R

N

V

I

V

I

A

S

Q

V

A

S

T

Q

R

R

E

E

Y

F

P

E

L

Q

H

I

V

Y

P

P

R

K

P

P

G

G

W

W

A

V

E

E

Q

H

N

D

P

P

E

M

D

E

W

I

Y

W

K

A

A

T

A

Q

C

S

G

S

A

T

I

L

A

V

T

E

I

V

I

L

S

T

K

K

G

A

-

D

I

I

S

S

P

S

G

D

Q

Q

I

I

Q

A

G

A

I

I

G

G

L

I

T

T

G

N

Q
|
Q

M
x
R

H
x
E

G

T

S

T

V

I

F

V

L

W

D

E

A

K

Q

E

-

T

G

G

K

K

V

P

I

I

R

Y

E

N

A

A

I

I

L

V

W
|
W

C

Q

D

C

Q

R

R

R

T

T

A

A

D

E

E

I

C

C

R

E

Q

H

I

L

T

K

D

R

L

D

V

G

G

L

E

E

S

D

K

Y

L

I

I

R

E

S

-

N

-

T

-

G

L

L

V

V

S

I

N

D

P

P

A
x
Y

L

-

A

-

G

-

F

F

T

S

A

G

P

T

K

K

I

V

L

K

W

W

L

I

R

L

N

D

H

H

E

V

P

E

E

G

N

S

Y

R

K

E

R

R

V

A

A

R

K

R

V

G

L

E

L

L

-

L

-

F

-

G

-

T

P

V

K

D

D

T

Y

W

I

L

S

I

W

W

R

K

M

M

T

T

G

Q

-

G

-

R

V

V

F

H

A

V

T

T

D

D

V

Y

S

T

D

N

A

A

S

S

G

R

M

T

L

M

L

L

L

F

D

N

V

I

V

H

N

T

R

L

K

D

W

W

S

D

S

D

E

K

M

M

L

L

A

E

A

V

L

L

E

D

I

I

P

P

A

R

A

E

W

M

L

L

A

P

E

E

V

V

F

R

E

R

S

S

P

S

D

E

V

V

V

Y

G

G

R

Q

V

T

S

N

A

I

R

G

G

G

A

K

A

G

E

G

T

T

G

-

L

-

P

-

E

-

G

R

I

I

P

P

V

I

V

S

A

G

G

I

A

A

G

G

D
|
D

N

Q

A

Q

A

A

G

A

A

L

V

F

G

G

N

Q

G

L

I

C

I

V

S

K

P

E

G

G

M

M

A

A

T

K

A

N

S

T

L

Y

G

G

T

T

S

G

G

C

V

F

V

M

F

L

T

M

P

N

S

T

Q

G

T

E

P

K

A

A

V

V

D

K

R

S

A

E

G

N

R

G

L

L

H

L

T

T

F

T

C

I

H

A

A

C

V

G

P

P

G

T

-

G

-

E

-

V

Q

N

W

Y

H

A

L

L

M

E

G

G

V

A

T

V

M

F

A

M

A

A

G

G

G

A

S

S

L

I

R

Q

W

W

Y

L

R

R

D

D

T

-

F

-

A

-

G

-

E

E

E

M

R

K

A

L

V

I

A

-

Q

-

L

-

T

-

G

-

R

N

D

D

P

A

Y

Y

E

D

L

-

M

-

S

S

D

E

E

Y

A

F

A

A

L

T

I

K

E

V

P

Q

G

N

A

T

D

N

G

G

L

V

I

Y

F

V

L

V

P

P

Y

A

L

F

S

T

G

G

E

L

R

G

T

A

P

P

H

Y

A

W

D

D

P

P

L

Y

A

A

R

R

G

G

V

A

F

I

V

F

G

G

M

L

N

T

L

R

K

G

H

V

T

N

K

A

G

N

H

H

F

I

V

I

R

R

S

A

I

T

M

L

E

E

G

S

V

I

A

A

F

Y

S

Q

L

T

K

R

D

D

T

V

L

L

E

E

I

A

M

M

-

Q

S

A

G

D

L

S

G

G

I

I

A

R

L

L

N

H

D

A

L

L

R

R

I

V

T

D

G

G

G

G

G

A

G

V

R

A

S

N

A

N

V

F

W

L

R

M

Q

Q

I

F

L

Q

A

S

D

D

V

I

F

L

N

G

M

T

P

R

L

V

Q

E

V

R

M

P

E

E

N

V

S

R

D

E

G

V

P

T

A

A

Y

L

G

G

A

A

A

A

I

Y

L

L

G

A

A

G

V

L

G

A

A

V

G

G

C

F

W

W

Q

Q

S

N

V

L

T

D

E

E

A

L

V

Q

A

E

A

K

T

A

C

V

S

I

R

E

Q

R

Q

E

L

F

Q

R

E

P

V

G

V

I

P

E

I

T

P

T

Q

E

N

R

A

N

A

Y

R

R

Y

Y

binding glycerol: Q81 (= Q80), R82 (≠ M81), E83 (≠ H82), W102 (= W100), Y134 (≠ A129), D244 (= D240)

P0A6F3 Glycerol kinase; ATP:glycerol 3-phosphotransferase; Glycerokinase; GK; EC 2.7.1.30 from Escherichia coli (strain K12) (see 10 papers)
27% identity, 95% coverage: 2:485/510 of query aligns to 4:484/502 of P0A6F3

query
sites
P0A6F3

Q

K

S

K

L

Y

F

I

L

V

G

A

I

L

D

D

I

Q

G

G

T
|
T

T

T

G

S

V

S

K
x
R

A

A

L

V

V

V

M

M

D

D

E

H

Q

D

G

A

R

N

V

I

V

I

A

S

Q

V

A

S

T

Q

R

R

E

E

Y

F

P

E

L

Q

H

I

V

Y

P

P

R

K

P

P

G

G

W

W

A

V

E

E

Q

H

N

D

P

P

E

M

D

E

W

I

Y

W

K

A

A

T

A

Q

C

S

G
x
S

A

T

I

L

A

V

T

E

I

V

I

L

S
x
A

K

K

G

A

-

D

I

I

S

S

P

S

G

D

Q

Q

I

I

Q

A

G

A

I

I

G

G

L

I

T

T

G

N

Q

Q

M
x
R

H
x
E

G

T

S

T

V

I

F

V

L

W

D

E

A

K

Q

E

-

T

G

G

K

K

V

P

I

I

R

Y

E

N

A

A

I

I

L

V

W

W

C

Q

D

C

Q

R

R

R

T

T

A

A

D

E

E

I

C

C

R

E

Q

H

I

L

T

K

D

R

L

D

V

G

G

L

E

E

S

D

K

Y

L

I

I

R

E

S

-

N

-

T

-

G

L

L

V

V

S

I

N

D

P

P

A
x
Y

L

-

A

-

G

-

F

F

T

S

A

G

P

T

K

K

I

V

L

K

W

W

L

I

R

L

N

D

H

H

E

V

P

E

E

G

N

S

Y

R

K

E

R

R

V

A

A

R

K

R

V

G

L

E

L

L

-

L

-

F

-

G

-

T

P

V

K

D

D

T

Y

W

I

L

S

I

W

W

R

K

M

M

T

T

G

Q

-

G

-

R

V

V

F

H

A

V

T

T

D

D

V

Y

S

T

D

N

A

A

S

S

G

R

M

T

L

M

L

L

L

F

D

N

V

I

V

H

N

T

R

L

K

D

W

W

S

D

S

D

E

K

M

M

L

L

A

E

A

V

L

L

E

D

I

I

P

P

A

R

A

E

W

M

L

L

A

P

E

E

V

V

F

R

E

R

S

S

P

S

D

E

V

V

V

Y

G

G

R

Q

V

T

S

N

A

I

R
x
G

G

G

A
x
K

A

G

E
x
G

T

T

G

-

L

-

P

-

E

-

G
x
R

I

I

P

P

V

I

V

S

A

G

G

I

A

A

G

G

D
|
D

N
x
Q

A

Q

A

A

G

A

A

L

V

F

G

G

N

Q

G

L

I

C

I

V

S

K

P

E

G

G

M

M

A

A

T

K

A

N

S

T

L

Y

G

G

T
|
T

S

G

G

C

V

F

V

M

F

L

T

M

P

N

S

T

Q

G

T

E

P

K

A

A

V

V

D

K

R

S

A

E

G

N

R

G

L

L

H

L

T

T

F

T

C

I

H

A

A

C

V

G

P

P

G

T

-

G

-

E

-

V

Q

N

W

Y

H

A

L

L

M

E

G
|
G

V

A

T

V

M

F

A

M

A

A

G
|
G

G

A

S

S

L

I

R

Q

W

W

Y

L

R

R

D

D

T

-

F

-

A

-

G

-

E

E

E

M

R

K

A

L

V

I

A

-

Q

-

L

-

T

-

G

-

R

N

D

D

P

A

Y

Y

E

D

L

-

M

-

S

S

D

E

E

Y

A

F

A

A

L

T

I

K

E

V

P

Q

G

N

A

T

D

N

G

G

L

V

I

Y

F

V

L

V

P

P

Y

A

L

F

S

T

G

G

E

L

R

G

T

A

P

P

H

Y

A

W

D

D

P

P

L

Y

A

A

R

R

G

G

V

A

F

I

V

F

G

G

M

L

N

T

L

R

K

G

H

V

T

N

K

A

G

N

H

H

F

I

V

I

R

R

S

A

I

T

M

L

E

E

G

S

V

I

A

A

F

Y

S

Q

L

T

K

R

D

D

T

V

L

L

E

E

I

A

M

M

-

Q

S

A

G

D

L

S

G

G

I

I

A

R

L

L

N

H

D

A

L

L

R

R

I

V

T

D

G

G

G
|
G

G

A

G

V

R

A

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x
N

A

N

V

F

W

L

R

M

Q

Q

I

F

L

Q

A

S

D

D

V

I

F

L

N

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M

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P

R

L

V

Q

E

V

R

M

P

E

E

N

V

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R

D

E

G

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A

A

Y

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G

A

A

A

A

I

Y

L

L

G

A

A

G

V

L

G

A

A

V

G

G

C

F

W

W

Q

Q

S

N

V

L

T

D

E

E

A

L

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Q

A

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A

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A

C

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V
x
I

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T

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x
E

N
x
R

A

N

A

Y

R

R

Y

Y

T14 (= T12) binding ADP; binding sn-glycerol 3-phosphate
R18 (≠ K16) binding ADP
S59 (≠ G57) mutation to W: Abolishes inhibition of GK by FBP via disruption of the dimer-tetramer assembly reaction. Inhibition by EIIA-Glc is unchanged compared to wild type. The activity of this mutant is significantly higher than wild-type, and the Michaelis constants are increased slightly compared to wild-type.
A66 (≠ S64) mutation to T: Although it completely abolishes FBP regulation and disrupts dimer-tetramer equilibrium, the crystal structure is essentially identical to the symmetric tetramer found in the FBP-bound form of the enzyme.
R84 (≠ M81) binding glycerol; binding sn-glycerol 3-phosphate
E85 (≠ H82) binding glycerol; binding sn-glycerol 3-phosphate
Y136 (≠ A129) binding glycerol; binding sn-glycerol 3-phosphate
G231 (≠ R221) mutation to D: Displays an increased enzymatic activity and a decreased allosteric regulation by FBP compared to wild-type. It displays a dimer form and is resistant to tetramer formation in the presence of FBP, whereas wild-type dimers are converted into inactive tetramers in the presence of FBP.
K233 (≠ A223) modified: N6-malonyllysine
G235 (≠ E225) binding beta-D-fructose 1,6-bisphosphate
R237 (≠ G231) binding beta-D-fructose 1,6-bisphosphate; mutation to A: Drastically reduces inhibition of GK by FBP and lowers, but did not eliminate, the ability of FBP to promote tetramer association.
D246 (= D240) binding glycerol; binding sn-glycerol 3-phosphate
Q247 (≠ N241) binding glycerol
T268 (= T262) binding ADP
G305 (= G296) mutation to S: In glpK22; abolishes glucose control of glycerol utilization.
G311 (= G302) binding ADP
G412 (= G413) binding ADP
N416 (≠ S417) binding ADP
I475 (≠ V476) mutation to D: It decreases Vmax to about 10% of the wild-type value and the affinity for substrate is increased about two- to fourfold. This mutation decreases the catalytic activity in a manner that is analogous to that obtained upon EIIA-Glc binding. It increases the affinity for FBP about fivefold.
E479 (≠ Q480) binding Zn(2+)
R480 (≠ N481) mutation to D: It decreases Vmax to about 10% of the wild-type value and the affinity for substrate is increased about two- to fourfold. This mutation decreases the catalytic activity in a manner that is analogous to that obtained upon EIIA-Glc binding. Regulation by FBP is not affected by this substitution. No inhibition by EIIA-Glc is observed, which is consistent with a decrease in affinity for EIIA-Glc of about 250-fold.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1glfO Crystal structures of escherichia coli glycerol kinase and the mutant a65t in an inactive tetramer: conformational changes and implications for allosteric regulation (see paper)
27% identity, 95% coverage: 2:485/510 of query aligns to 2:482/498 of 1glfO

query
sites
1glfO

Q

K

S

K

L

Y

F

I

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A

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D

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x
R

A

A

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M

D

D

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D

G

A

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N

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W

A

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A

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A

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A

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A

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K

G

A

-

D

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I

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S

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S

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D

Q

Q

I

I

Q

A

G

A

I

I

G

G

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N

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x
R

H
x
E

G

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A

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E

-

T

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G

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A

A

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W

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R

L

D

V

G

G

L

E

E

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D

K

Y

L

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I

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-

N

-

T

-

G

L

L

V

V

S

I

N

D

P

P

A
x
Y

L

-

A

-

G

-

F

F

T

S

A

G

P

T

K

K

I

V

L

K

W

W

L

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L

N

D

H

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P

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N

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Y

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E

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A

A

R

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R

V

G

L

E

L

L

-

L

-

F

-

G

-

T

P

V

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D

D

T

Y

W

I

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I

W

W

R

K

M

M

T

T

G

Q

-

G

-

R

V

V

F

H

A

V

T

T

D

D

V

Y

S

T

D

N

A

A

S

S

G

R

M

T

L

M

L

L

L

F

D

N

V

I

V

H

N

T

R

L

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D

W

W

S

D

S

D

E

K

M

M

L

L

A

E

A

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L

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D

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A

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A

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M

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A

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E

E

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V

F

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E

R

S

S

P

S

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E

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V

Y

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N

A

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R

G

G

G

A

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A
x
G

E
x
G

T

T

G

-

L

-

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-

E

-

G
x
R

I

I

P

P

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S

A

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G

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A

A

G

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D
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D

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Q

A

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A

A

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A

A

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N

Q

G

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M

A

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A

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G
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G

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T

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F

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A

A

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A

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G

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W

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R

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D

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-

F

-

A

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-

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A

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A

-

Q

-

L

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T

-

G

-

R

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D

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A

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Y

E

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-

M

-

S

S

D

E

E

Y

A

F

A

A

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E

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G

N

A

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N

G

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Y

A

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D

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P

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Y

A

A

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A

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T

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-

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A

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G

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x
A

G

V

R

A

S

N

A

N

V

F

W

L

R

M

Q

Q

I

F

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A

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D

D

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A

A

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G

A

A

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Y

L

L

G

A

A

G

V

L

G

A

A

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G

G

C

F

W

W

Q

Q

S

N

V

L

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D

E

E

A

L

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Q

A

E

A

K

T

A

C

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S

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R

Q

E

L

F

Q

R

E

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V

G

V

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P

E

I

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P

T

Q

E

N

R

A

N

A

Y

R

R

Y

Y

binding adenosine-5'-diphosphate: R16 (≠ K16), G265 (= G261), T266 (= T262), G309 (= G302), G410 (= G413), A411 (≠ G414)
binding glycerol: R82 (≠ M81), E83 (≠ H82), Y134 (≠ A129), D244 (= D240)
binding phosphate ion: G232 (≠ A224), G233 (≠ E225), R235 (≠ G231)

1bo5O Crystal structure of the complex between escherichia coli glycerol kinase and the allosteric regulator fructose 1,6-bisphosphate. (see paper)
27% identity, 95% coverage: 2:485/510 of query aligns to 2:482/498 of 1bo5O

query
sites
1bo5O

Q

K

S

K

L

Y

F

I

L

V

G

A

I

L

D

D

I

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G

G

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A

A

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M

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D

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D

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A

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W

A

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A

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A

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A

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D

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S

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D

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Q

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A

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A

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G

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N

Q

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M
x
R

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x
E

G

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T

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D

E

A

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E

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T

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G

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A

A

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W
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W

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C

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R

L

D

V

G

G

L

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D

K

Y

L

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I

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x
Y

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-

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-

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A

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W

L

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A

A

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G

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E

L

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-

L

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D

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W

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M

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Q

-

G

-

R

V

V

F

H

A

V

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T

D

D

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Y

S

T

D

N

A

A

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S

G

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M

L

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L

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W

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D

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M

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A

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A

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M

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A

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G

G

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A
x
G

E
x
G

T

T

G

-

L

-

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-

E

-

G
x
R

I

I

P

P

V

I

V

S

A

G

G

I

A

A

G

G

D
|
D

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x
Q

A

Q

A

A

G

A

A

L

V

F

G

G

N

Q

G

L

I

C

I

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E

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G

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M

A

A

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A

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Y

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x
F

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F

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A

A

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A

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-

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A

A

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A

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A

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-

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Y

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-

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D

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E

Y

A

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A

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A

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N

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A

A

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A

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T

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-

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A

L

L

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R

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D

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A

G

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N

A

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V

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A

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D

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E

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A

A

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L

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A

A

A

A

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Y

L

L

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A

A

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L

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A

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G

C

F

W

W

Q

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V

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T

D

E

E

A

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A

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A

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R

Q

E

L

F

Q

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E

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V

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V

I

P

E

I

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T

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R

A

N

A

Y

R

R

Y

Y

binding 1,6-di-O-phosphono-beta-D-fructofuranose: G232 (≠ A224), G233 (≠ E225), R235 (≠ G231)
binding glycerol: R82 (≠ M81), E83 (≠ H82), W102 (= W100), Y134 (≠ A129), D244 (= D240), Q245 (≠ N241), F269 (≠ V265)

O34154 Glycerol kinase; ATP:glycerol 3-phosphotransferase; Glycerokinase; GK; EC 2.7.1.30 from Enterococcus faecalis (strain ATCC 700802 / V583) (see 2 papers)
26% identity, 95% coverage: 2:486/510 of query aligns to 4:484/501 of O34154

query
sites
O34154

Q

E

S

K

L

Y

F

I

L

M

G

A

I

I

D

D

I

Q

G

G

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A

A

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F

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D

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K

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V

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A

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K

E

E

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F

P

P

R

N

P

A

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G

W

W

A

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E

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H

N

N

P

A

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W

I

Y

W

K

N

A

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G

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Q

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D

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Q

E

H231 (≠ G222) modified: Phosphohistidine; by HPr

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1gllO Escherichia coli glycerol kinase mutant with bound atp analog showing substantial domain motion (see paper)
27% identity, 95% coverage: 2:485/510 of query aligns to 2:478/494 of 1gllO

query
sites
1gllO

Q

K

S

K

L

Y

F

I

L

V

G

A

I

L

D

D

I

Q

G

G

T
|
T

T
|
T

G

S

V

S

K

R

A

A

L

V

V

V

M

M

D

D

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H

Q

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A

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N

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P

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H

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P

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K

P

P

G

G

W

W

A

V

E

E

Q

H

N

D

P

P

-

M

E

E

D

I

W

W

Y

A

K

T

A

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A

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G

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A

L

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A

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K

A

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D

I

I

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D

Q

Q

I

I

Q

A

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A

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G

G

L

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T

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N

Q

Q

M
x
R

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x
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V

I

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D

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A

K

Q

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G

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K

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W

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D

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Q

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I

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D

P

P

A
x
Y

L

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A

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G

-

F

F

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A

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P

T

K

K

I

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W

W

L

I

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W

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D

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G

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D
|
D

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A

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G

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A

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|
G

G

A

S

S

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I

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Q

W

W

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D

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D

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S

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L

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L

L

R

R

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D

G

G

G
|
G

G

A

G

V

R

A

S
x
N

A

N

V

F

W

L

R

M

Q

Q

I

F

L

Q

A

S

D

D

V

I

F

L

N

G

M

T

P

R

L

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Q

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M

P

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N

V

S

R

D

E

G

V

P

T

A

A

Y

L

G

G

A

A

A

A

I

Y

L

L

G

A

A

G

V

L

G

A

A

V

G

G

C

F

W

W

Q

Q

S

N

V

L

T

D

E

E

A

L

V

Q

A

E

A

K

T

A

C

V

S

I

R

E

Q

R

Q

E

L

F

Q

R

E

P

V

G

V

I

P

E

I

T

P

T

Q

E

N

R

A

N

A

Y

R

R

Y

Y

binding phosphomethylphosphonic acid adenylate ester: T12 (= T12), T13 (= T13), G261 (= G261), T262 (= T262), G305 (= G302), I308 (≠ L305), Q309 (≠ R306), A321 (≠ P327), G406 (= G413), N410 (≠ S417)
binding glycerol: R82 (≠ M81), E83 (≠ H82), Y134 (≠ A129), D240 (= D240), Q241 (≠ N241), F265 (≠ V265)

1gljO Escherichia coli glycerol kinase mutant with bound atp analog showing substantial domain motion (see paper)
27% identity, 95% coverage: 2:485/510 of query aligns to 2:478/494 of 1gljO

query
sites
1gljO

Q

K

S

K

L

Y

F

I

L

V

G

A

I

L

D

D

I

Q

G

G

T
|
T

T
|
T

G

S

V

S

K

R

A

A

L

V

V

V

M

M

D

D

E

H

Q

D

G

A

R

N

V

I

V

I

A

S

Q

V

A

S

T

Q

R

R

E

E

Y

F

P

E

L

Q

H

I

V

Y

P

P

R

K

P

P

G

G

W

W

A

V

E

E

Q

H

N

D

P

P

-

M

E

E

D

I

W

W

Y

A

K

T

A

Q

A

S

C

W

G

T

A

L

I

V

A

E

T

V

I

L

I

A

S

K

K

A

G

D

I

I

S

S

P

S

G

D

Q

Q

I

I

Q

A

G

A

I

I

G

G

L

I

T

T

G

N

Q

Q

M
x
R

H
x
E

G

T

S

T

V

I

F

V

L

W

D

E

A

K

Q

E

-

T

G

G

K

K

V

P

I

I

R

Y

E

N

A

A

I

I

L

V

W
|
W

C

Q

D

C

Q

R

R

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T

T

A

A

D

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E

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C

C

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H

I

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D

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L

D

V

G

G

L

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S

D

K

Y

L

I

I

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-

N

-

T

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G

L

L

V

V

S

I

N

D

P

P

A
x
Y

L

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A

-

G

-

F

F

T

S

A

G

P

T

K

K

I

V

L

K

W

W

L

I

R

L

N

D

H

H

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V

P

E

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G

N

S

Y

R

K

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R

R

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A

A

R

K

R

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G

L

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L

L

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L

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F

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-

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P

V

K

D

D

T

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W

I

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I

W

W

R

K

M

M

T

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Q

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G

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V

V

F

H

A

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D

D

V

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S

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D

N

A

A

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M

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L

M

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L

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D

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W

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D

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M

M

L

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G

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S

A

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G

I

A

A

G

G

D
|
D

N

Q

A

Q

A

A

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A

A

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G

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M

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|
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|
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S

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W

W

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D

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F

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M

R

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A

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D

P
x
A

Y

Y

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M

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S

D

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E

Y

A

F

A

A

L

T

I

K

E

V

P

Q

G

N

A

T

D

N

G

G

L

V

I

Y

F

V

L

V

P

P

Y

A

L

F

S

T

G

G

E

L

R

G

T

A

P

P

H

Y

A

W

D

D

P

P

L

Y

A

A

R

R

G

G

V

A

F

I

V

F

G

G

M

L

N

T

L

R

K

G

H

V

T

N

K

A

G

N

H

H

F

I

V

I

R

R

S

A

I

T

M

L

E

E

G

S

V

I

A

A

F

Y

S

Q

L

T

K

R

D

D

T

V

L

L

E

E

I

A

M

M

-

Q

S

A

G

D

L

S

G

G

I

I

A

R

L

L

N

H

D

A

L

L

R

R

I

V

T

D

G

G

G
|
G

G
x
A

G

V

R

A

S

N

A

N

V

F

W

L

R

M

Q

Q

I

F

L

Q

A

S

D

D

V

I

F

L

N

G

M

T

P

R

L

V

Q

E

V

R

M

P

E

E

N

V

S

R

D

E

G

V

P

T

A

A

Y

L

G

G

A

A

A

A

I

Y

L

L

G

A

A

G

V

L

G

A

A

V

G

G

C

F

W

W

Q

Q

S

N

V

L

T

D

E

E

A

L

V

Q

A

E

A

K

T

A

C

V

S

I

R

E

Q

R

Q

E

L

F

Q

R

E

P

V

G

V

I

P

E

I

T

P

T

Q

E

N

R

A

N

A

Y

R

R

Y

Y

binding gamma-arsono-beta, gamma-methyleneadenosine-5'-diphosphate: T12 (= T12), T13 (= T13), G261 (= G261), T262 (= T262), G305 (= G302), Q309 (≠ R306), A321 (≠ P327), G406 (= G413), A407 (≠ G414)
binding glycerol: R82 (≠ M81), E83 (≠ H82), W102 (= W100), Y134 (≠ A129), D240 (= D240), F265 (≠ V265)

1bwfO Escherichia coli glycerol kinase mutant with bound atp analog showing substantial domain motion (see paper)
27% identity, 95% coverage: 2:485/510 of query aligns to 2:478/494 of 1bwfO

query
sites
1bwfO

Q

K

S

K

L

Y

F

I

L

V

G

A

I

L

D

D

I

Q

G

G

T
|
T

T
|
T

G

S

V

S

K

R

A

A

L

V

V

V

M

M

D

D

E

H

Q

D

G

A

R

N

V

I

V

I

A

S

Q

V

A

S

T

Q

R

R

E

E

Y

F

P

E

L

Q

H

I

V

Y

P

P

R

K

P

P

G

G

W

W

A

V

E

E

Q

H

N

D

P

P

-

M

E

E

D

I

W

W

Y

A

K

T

A

Q

A

S

C

W

G

T

A

L

I

V

A

E

T

V

I

L

I

A

S

K

K

A

G

D

I

I

S

S

P

S

G

D

Q

Q

I

I

Q

A

G

A

I

I

G

G

L

I

T

T

G

N

Q

Q

M
x
R

H
x
E

G

T

S

T

V

I

F

V

L

W

D

E

A

K

Q

E

-

T

G

G

K

K

V

P

I

I

R

Y

E

N

A

A

I

I

L

V

W
|
W

C

Q

D

C

Q

R

R

R

T

T

A

A

D

E

E

I

C

C

R

E

Q

H

I

L

T

K

D

R

L

D

V

G

G

L

E

E

S

D

K

Y

L

I

I

R

E

S

-

N

-

T

-

G

L

L

V

V

S

I

N

D

P

P

A
x
Y

L

-

A

-

G

-

F

F

T

S

A

G

P

T

K

K

I

V

L

K

W

W

L

I

R

L

N

D

H

H

E

V

P

E

E

G

N

S

Y

R

K

E

R

R

V

A

A

R

K

R

V

G

L

E

L

L

-

L

-

F

-

G

-

T

P

V

K

D

D

T

Y

W

I

L

S

I

W

W

R

K

M

M

T

T

G

Q

-

G

-

R

V

V

F

H

A

V

T

T

D

D

V

Y

S

T

D

N

A

A

S

S

G

R

M

T

L

M

L

L

L

F

D

N

V

I

V

H

N

T

R

L

K

D

W

W

S

D

S

D

E

K

M

M

L

L

A

E

A

V

L

L

E

D

I

I

P

P

A

R

A

E

W

M

L

L

A

P

E

E

V

V

F

R

E

R

S

S

P

S

D

E

V

V

V

Y

G

G

R

-

V

-

S

-

A

-

R

-

G

-

A

-

A

-

E

Q

T

T

G

N

L

I

P

G

E

T

G

R

I

I

P

P

V

I

V

S

A

G

G

I

A

A

G

G

D
|
D

N
x
Q

A

Q

A

A

G

A

A

L

V

F

G

G

N

Q

G

L

I

C

I

V

S

K

P

E

G

G

M

M

A

A

T

K

A

N

S

T

L

Y

G

G

T
|
T

S

G

G

C

V
x
F

V

M

F

L

T

M

P

N

S

T

Q

G

T

E

P

K

A

A

V

V

D

K

R

S

A

E

G

N

R

G

L

L

H

L

T

T

F

T

C

I

H

A

A

C

V

G

P

P

G

T

-

G

-

E

-

V

Q

N

W

Y

H

A

L

L

M

E

G

G

V

A

T

V

M

F

A

M

A

A

G
|
G

G

A

S

S

L
x
I

R
x
Q

W

W

Y

L

R

R

D

D

T

-

F

-

A

-

G

-

E

E

E

M

R

K

A

L

V

I

A

-

Q

-

L

-

T

-

G

-

R

N

D

D

P
x
A

Y

Y

E

D

L

-

M

-

S

S

D

E

E

Y

A

F

A

A

L

T

I

K

E

V

P

Q

G

N

A

T

D

N

G

G

L

V

I

Y

F

V

L

V

P

P

Y

A

L

F

S

T

G

G

E

L

R

G

T

A

P

P

H

Y

A

W

D

D

P

P

L

Y

A

A

R

R

G

G

V

A

F

I

V

F

G

G

M

L

N

T

L

R

K

G

H

V

T

N

K

A

G

N

H

H

F

I

V

I

R

R

S

A

I

T

M

L

E

E

G

S

V

I

A

A

F

Y

S

Q

L

T

K

R

D

D

T

V

L

L

E

E

I

A

M

M

-

Q

S

A

G

D

L

S

G

G

I

I

A

R

L

L

N

H

D

A

L

L

R

R

I

V

T

D

G

G

G
|
G

G

A

G

V

R

A

S
x
N

A

N

V

F

W

L

R

M

Q

Q

I

F

L

Q

A

S

D

D

V

I

F

L

N

G

M

T

P

R

L

V

Q

E

V

R

M

P

E

E

N

V

S

R

D

E

G

V

P

T

A

A

Y

L

G

G

A

A

A

A

I

Y

L

L

G

A

A

G

V

L

G

A

A

V

G

G

C

F

W

W

Q

Q

S

N

V

L

T

D

E

E

A

L

V

Q

A

E

A

K

T

A

C

V

S

I

R

E

Q

R

Q

E

L

F

Q

R

E

P

V

G

V

I

P

E

I

T

P

T

Q

E

N

R

A

N

A

Y

R

R

Y

Y

binding phosphodifluoromethylphosphonic acid-adenylate ester: T12 (= T12), T13 (= T13), T262 (= T262), G305 (= G302), I308 (≠ L305), Q309 (≠ R306), A321 (≠ P327), G406 (= G413), N410 (≠ S417)
binding glycerol: R82 (≠ M81), E83 (≠ H82), W102 (= W100), Y134 (≠ A129), D240 (= D240), Q241 (≠ N241), F265 (≠ V265)

3kzbA Crystal structure of xylulokinase from chromobacterium violaceum
31% identity, 87% coverage: 8:451/510 of query aligns to 9:449/498 of 3kzbA

query
sites
3kzbA

I

F

D

D

I

I

G

G

T

T

T

T

G

E

V

V

K

K

A

A

L

A

V

L

M

A

D

D

E

R

Q

D

G

G

R

G

V

L

V

H

A

F

Q

Q

A

R

T

S

R

I

E

A

Y

L

P

E

L

T

H

Y

V

G

P

D

R

G

P

N

G

G

W

P

A

V

E

E

Q

Q

N

D

P

A

E

G

D

D

W

W

Y

Y

K

D

A

A

A

V

C

Q

G

R

A

I

I

A

A

S

T

S

I

W

I

W

S

Q

K

S

G

G

I

V

S

D

P

A

G

R

Q

R

I

V

Q

S

G

A

I

I

G

V

L

L

T

S

G

G

Q

Q

M

M

H

Q

G

N

S

F

V

L

F

P

L

L

D

D

A

Q

Q

D

G

H

K

E

V

P

I

L

R

H

E

R

A

A

I

V

L

L

W

Y

C

S

D

D

Q

K

R

R

T

P

A

L

D

K

E

E

C

A

R

E

Q

E

I

I

T

N

D

A

L

R

V

H

G

G

E

A

S

D

K

N

L

L

I

W

E

S

L

A

V

L

S

E

N

N

P

P

A

M

L

T

A

A

G

A

F

S

T

I

A

L

P

P

K

K

I

L

L

V

W

F

L

W

R

R

N

A

H

S

E

F

P

P

E

Q

N

A

Y

F

K

G

R

R

V

L

A

R

K

H

V

V

L

V

L

L

-

G

P

A

K

K

D

D

Y

Y

I

V

S

V

W

L

R

R

M

L

T

T

G

G

V

R

F

H

A

A

T

T

D

D

V

R

S

T

D

N

A

A

S

S

G

T

M

T

L

G

L

L

L

Y

D

R

V

P

V

K

N

D

R

D

K

A

W

W

S

H

S

V

E

E

M

L

L

L

A

A

A

D

L

Y

E

G

I

F

P

S

A

L

A

D

W

L

L

M

A

P

E

R

V

L

F

L

E

E

S

P

P

G

D

E

V

Q

V

V

G

G

R

G

V

V

S

S

A

A

R

L

G

A

A

A

A

R

E

Q

T

T

G

G

L

F

P

V

E

S

G

G

I

T

P

P

V

V

V

L

A

C

G

G

A

L

G

G

D

D

N

A

A

G

A

A

G

A

A

T

V

L

G

G

N

V

G

G

I

V

I

L

S

D

P

D

G

E

M

D

A

A

T

Y

A

L

S

H

L

L

G
|
G

T
|
T

S

T

G

G

V

W

V

L

F

A

T

R

P

L

S

T

Q

Q

T

T

P

D

A

P

V

V

D

G

R

D

-

M

-

P

A

V

G

G

R

T

L

I

H

F

T

R

F

L

C

A

H

G

A

I

V

I

P

A

G

G

Q

K

W

T

H

L

L

Q

M

V

G

A

V

P

T

V

M

L

A

N

A

A

G
|
G

G

N

S

I

L

L

R
x
Q

W

W

Y

A

R

L

D

-

T

T

F

L

A

V

G

G

E

H

E

R

R

-

A

-

V

-

A

-

Q

-

L

-

T

P

G

G

R

E

D

D

P

C

Y

A

E

E

L

Y

M
x
F

S

H

D

M

E

A

A

A

A

A

L

E

I

V

E

Q

-

G

-

V

P

T

G

V

A

P

D

D

G

G

L

L

I

L

F

F

L

V

P

P

Y

Y

L

L

S

H

G

A

E

E

R

R

T

C

P

P

H

V

A

E

D

L

P

P

L

A

A

P

R

R

G

G

V

A

F

L

V

L

G

G

M

V

N

T

L

G

K

A

H

T

T

T

K

R

G

A

H

Q

F

I

V

L

R

L

S

A

I

V

M

L

E

E

G

G

V

A

A

A

F

L

S

S

L

L

K

R

D

W

T

C

L

A

E

E

I

L

M

L

S

G

G

M

L

E

G

K

I

V

A

G

L

L

N

-

D

-

L

L

R

K

I

V

T

V

G

G

G
|
G

G

G

G

A

R
|
R

S

S

A

E

V

A

W

W

R

L

Q

R

I

M

L

I

A

A

D

D

V

N

F

L

N

N

M

V

P

S

L

L

Q

L

V

V

M

K

E

P

N

D

S

A

D

H

-

L

G

H

P

P

A

L

Y

R

G

G

A

L

A

A

I

A

L

L

G

A

A

A

V

V

binding adenosine-5'-diphosphate: G263 (= G261), T264 (= T262), G306 (= G302), Q310 (≠ R306), F328 (≠ M331), G410 (= G413), R413 (= R416)

1gldG Cation promoted association (cpa) of a regulatory and target protein is controlled by phosphorylation (see paper)
27% identity, 94% coverage: 5:485/510 of query aligns to 3:473/489 of 1gldG

query
sites
1gldG

F

I

L

V

G

A

I

L

D
|
D

I

Q

G

G

T
|
T

T

T

G

S

V

S

K
x
R

A

A

L

V

V

V

M

M

D

D

E

H

Q

D

G

A

R

N

V

I

V

I

A

S

Q

V

A

S

T

Q

R

R

E

E

Y

F

P

E

L

Q

H

I

V

Y

P

P

R

K

P

P

G

G

W

W

A

V

E

E

Q

H

N

D

P

P

E

M

D

E

W

I

Y

W

K

A

A

T

A

Q

C

S

G

S

A

T

I

L

A

V

T

E

I

V

I

L

S

A

K

K

G

A

-

D

I

I

S

S

P

S

G

D

Q

Q

I

I

Q

A

G

A

I

I

G

G

L

I

T

T

G

N

Q

Q

M
x
R

H
x
E

G

T

S

T

V

I

F

V

L

W

D

E

A

K

Q

E

-

T

G

G

K

K

V

P

I

I

R

Y

E

N

A

A

I

I

L

V

W

W

C

Q

D

C

Q

R

R

R

T

T

A

A

D

E

E

I

C

C

R

E

Q

H

I

L

T

K

D

R

L

D

V

G

G

L

E

E

S

D

K

Y

L

I

I

R

E

S

-

N

-

T

-

G

L

L

V

V

S

I

N

D

P

P

A
x
Y

L

-

A

-

G

-

F

F

T

S

A

G

P

T

K

K

I

V

L

K

W

W

L

I

R

L

N

D

H

H

E

V

P

E

E

G

N

S

Y

R

K

E

R

R

V

A

A

R

K

R

V

G

L

E

L

L

-

L

-

F

-

G

-

T

P

V

K

D

D

T

Y

W

I

L

S

I

W

W

R

K

M

M

T

T

G

Q

-

G

-

R

V

V

F

H

A

V

T

T

D

D

V

Y

S

T

D

N

A

A

S

S

G

R

M

T

L

M

L

L

L

F

D

N

V

I

V

H

N

T

R

L

K

D

W

W

S

D

S

D

E

K

M

M

L

L

A

E

A

V

L

L

E

D

I

I

P

P

A

R

A

E

W

M

L

L

A

P

E

E

V

V

F

R

E

R

S

S

P

S

D

E

V

V

V

Y

G

G

R

Q

V

T

S

N

A

I

R

-

G

-

A

-

A

-

E

-

T

-

G

-

L

-

P

-

E

-

G

-

I

I

P

P

V

I

V

S

A

G

G

I

A

A

G

G

D
|
D

N

Q

A

Q

A

A

G

A

A

L

V

F

G

G

N

Q

G

L

I

C

I

V

S

K

P

E

G

G

M

M

A

A

T

K

A

N

S

T

L

Y

G
|
G

T
|
T

S

G

G

C

V
x
F

V

M

F

L

T

M

P

N

S

T

Q

G

T

E

P

K

A

A

V

V

D

K

R

S

A

E

G

N

R

G

L

L

H

L

T

T

F

T

C

I

H

A

A

C

V

G

P

P

G

T

-

G

-

E

-

V

Q

N

W

Y

H

A

L

L

M

E

G

G

V

A

T

V

M

F

A

M

A

A

G
|
G

G

A

S

S

L

I

R

Q

W

W

Y

L

R

R

D

D

T

-

F

-

A

-

G

-

E

E

E

M

R

K

A

L

V

I

A

-

Q

-

L

-

T

-

G

-

R

N

D

D

P
x
A

Y

Y

E

D

L

-

M

-

S

S

D

E

E

Y

A

F

A

A

L

T

I

K

E

V

P

Q

G

N

A

T

D

N

G

G

L

V

I

Y

F

V

L

V

P

P

Y

A

L

F

S

T

G

G

E

L

R

G

T

A

P

P

H

Y

A

W

D

D

P

P

L

Y

A

A

R

R

G

G

V

A

F

I

V

F

G

G

M

L

N

T

L

R

K

G

H

V

T

N

K

A

G

N

H

H

F

I

V

I

R

R

S

A

I

T

M

L

E

E

G

S

V

I

A

A

F

Y

S

Q

L

T

K

R

D

D

T

V

L

L

E

E

I

A

M

M

-

Q

S

A

G

D

L

S

G

G

I

I

A

R

L

L

N

H

D

A

L

L

R

R

I

V

T

D

G

G

G
|
G

G
x
A

G

V

R

A

S
x
N

A

N

V

F

W

L

R

M

Q

Q

I

F

L

Q

A

S

D

D

V

I

F

L

N

G

M

T

P

R

L

V

Q

E

V

R

M

P

E

E

N

V

S

R

D

E

G

V

P

T

A

A

Y

L

G

G

A

A

A

A

I

Y

L

L

G

A

A

G

V

L

G

A

A

V

G

G

C

F

W

W

Q

Q

S

N

V

L

T

D

E

E

A

L

V

Q

A

E

A

K

T

A

C

V

S

I

R

E

Q

R

Q

E

L

F

Q

R

E

P

V

G

V

I

P

E

I

T

P

T

Q

E

N

R

A

N

A

Y

R

R

Y

Y

binding adenosine-5'-diphosphate: R14 (≠ K16), G256 (= G261), T257 (= T262), G300 (= G302), A316 (≠ P327), G401 (= G413), A402 (≠ G414), N405 (≠ S417)
binding glyceraldehyde-3-phosphate: T10 (= T12), R80 (≠ M81), E81 (≠ H82), Y132 (≠ A129), D235 (= D240), F260 (≠ V265)
binding manganese (ii) ion: D7 (= D9), R14 (≠ K16)

1glcG Cation promoted association (cpa) of a regulatory and target protein is controlled by phosphorylation (see paper)
27% identity, 94% coverage: 5:485/510 of query aligns to 3:473/489 of 1glcG

query
sites
1glcG

F

I

L

V

G

A

I

L

D

D

I

Q

G

G

T
|
T

T

T

G

S

V

S

K

R

A

A

L

V

V

V

M

M

D

D

E

H

Q

D

G

A

R

N

V

I

V

I

A

S

Q

V

A

S

T

Q

R

R

E

E

Y

F

P

E

L

Q

H

I

V

Y

P

P

R

K

P

P

G

G

W

W

A

V

E

E

Q

H

N

D

P

P

E

M

D

E

W

I

Y

W

K

A

A

T

A

Q

C

S

G

S

A

T

I

L

A

V

T

E

I

V

I

L

S

A

K

K

G

A

-

D

I

I

S

S

P

S

G

D

Q

Q

I

I

Q

A

G

A

I

I

G

G

L

I

T

T

G

N

Q

Q

M
x
R

H
x
E

G

T

S

T

V

I

F

V

L

W

D

E

A

K

Q

E

-

T

G

G

K

K

V

P

I

I

R

Y

E

N

A

A

I

I

L

V

W
|
W

C

Q

D

C

Q

R

R

R

T

T

A

A

D

E

E

I

C

C

R

E

Q

H

I

L

T

K

D

R

L

D

V

G

G

L

E

E

S

D

K

Y

L

I

I

R

E

S

-

N

-

T

-

G

L

L

V

V

S

I

N

D

P

P

A
x
Y

L

-

A

-

G

-

F

F

T

S

A

G

P

T

K

K

I

V

L

K

W

W

L

I

R

L

N

D

H

H

E

V

P

E

E

G

N

S

Y

R

K

E

R

R

V

A

A

R

K

R

V

G

L

E

L

L

-

L

-

F

-

G

-

T

P

V

K

D

D

T

Y

W

I

L

S

I

W

W

R

K

M

M

T

T

G

Q

-

G

-

R

V

V

F

H

A

V

T

T

D

D

V

Y

S

T

D

N

A

A

S

S

G

R

M

T

L

M

L

L

L

F

D

N

V

I

V

H

N

T

R

L

K

D

W

W

S

D

S

D

E

K

M

M

L

L

A

E

A

V

L

L

E

D

I

I

P

P

A

R

A

E

W

M

L

L

A

P

E

E

V

V

F

R

E

R

S

S

P

S

D

E

V

V

V

Y

G

G

R

Q

V

T

S

N

A

I

R

-

G

-

A

-

A

-

E

-

T

-

G

-

L

-

P

-

E

-

G

-

I

I

P

P

V

I

V

S

A

G

G

I

A

A

G

G

D
|
D

N

Q

A

Q

A

A

G

A

A

L

V

F

G

G

N

Q

G

L

I

C

I

V

S

K

P

E

G

G

M

M

A

A

T

K

A

N

S

T

L

Y

G
|
G

T
|
T

S

G

G

C

V
x
F

V

M

F

L

T

M

P

N

S

T

Q

G

T

E

P

K

A

A

V

V

D

K

R

S

A

E

G

N

R

G

L

L

H

L

T

T

F

T

C

I

H

A

A

C

V

G

P

P

G

T

-

G

-

E

-

V

Q

N

W

Y

H

A

L

L

M

E

G

G

V

A

T

V

M

F

A

M

A

A

G
|
G

G

A

S

S

L

I

R

Q

W

W

Y

L

R

R

D

D

T

-

F

-

A

-

G

-

E

E

E

M

R

K

A

L

V

I

A

-

Q

-

L

-

T

-

G

-

R

N

D

D

P
x
A

Y

Y

E

D

L

-

M

-

S

S

D

E

E

Y

A

F

A

A

L

T

I

K

E

V

P

Q

G

N

A

T

D

N

G

G

L

V

I

Y

F

V

L

V

P

P

Y

A

L

F

S

T

G

G

E

L

R

G

T

A

P

P

H

Y

A

W

D

D

P

P

L

Y

A

A

R

R

G

G

V

A

F

I

V

F

G

G

M

L

N

T

L

R

K

G

H

V

T

N

K

A

G

N

H

H

F

I

V

I

R

R

S

A

I

T

M

L

E

E

G

S

V

I

A

A

F

Y

S

Q

L

T

K

R

D

D

T

V

L

L

E

E

I

A

M

M

-

Q

S

A

G

D

L

S

G

G

I

I

A

R

L

L

N

H

D

A

L

L

R

R

I

V

T

D

G

G

G
|
G

G
x
A

G

V

R

A

S
x
N

A

N

V

F

W

L

R

M

Q

Q

I

F

L

Q

A

S

D

D

V

I

F

L

N

G

M

T

P

R

L

V

Q

E

V

R

M

P

E

E

N

V

S

R

D

E

G

V

P

T

A

A

Y

L

G

G

A

A

A

A

I

Y

L

L

G

A

A

G

V

L

G

A

A

V

G

G

C

F

W

W

Q

Q

S

N

V

L

T

D

E

E

A

L

V

Q

A

E

A

K

T

A

C

V

S

I

R

E

Q

R

Q

E

L

F

Q

R

E

P

V

G

V

I

P

E

I

T

P

T

Q

E

N

R

A

N

A

Y

R

R

Y

Y

binding adenosine-5'-diphosphate: G256 (= G261), T257 (= T262), G300 (= G302), A316 (≠ P327), G401 (= G413), A402 (≠ G414), N405 (≠ S417)
binding glyceraldehyde-3-phosphate: T10 (= T12), R80 (≠ M81), E81 (≠ H82), W100 (= W100), Y132 (≠ A129), D235 (= D240), F260 (≠ V265)

1glbG Structure of the regulatory complex of escherichia coli iiiglc with glycerol kinase (see paper)
27% identity, 94% coverage: 5:485/510 of query aligns to 3:473/489 of 1glbG

query
sites
1glbG

F

I

L

V

G

A

I

L

D

D

I

Q

G

G

T

T

T

T

G

S

V

S

K
x
R

A

A

L

V

V

V

M

M

D

D

E

H

Q

D

G

A

R

N

V

I

V

I

A

S

Q

V

A

S

T

Q

R

R

E

E

Y

F

P

E

L

Q

H

I

V

Y

P

P

R

K

P

P

G

G

W

W

A

V

E

E

Q

H

N

D

P

P

E

M

D

E

W

I

Y

W

K

A

A

T

A

Q

C

S

G

S

A

T

I

L

A

V

T

E

I

V

I

L

S

A

K

K

G

A

-

D

I

I

S

S

P

S

G

D

Q

Q

I

I

Q

A

G

A

I

I

G

G

L

I

T

T

G

N

Q

Q

M
x
R

H
x
E

G

T

S

T

V

I

F

V

L

W

D

E

A

K

Q

E

-

T

G

G

K

K

V

P

I

I

R

Y

E

N

A

A

I

I

L

V

W
|
W

C

Q

D

C

Q

R

R

R

T

T

A

A

D

E

E

I

C

C

R

E

Q

H

I

L

T

K

D

R

L

D

V

G

G

L

E

E

S

D

K

Y

L

I

I

R

E

S

-

N

-

T

-

G

L

L

V

V

S

I

N

D

P

P

A
x
Y

L

-

A

-

G

-

F

F

T

S

A

G

P

T

K

K

I

V

L

K

W

W

L

I

R

L

N

D

H

H

E

V

P

E

E

G

N

S

Y

R

K

E

R

R

V

A

A

R

K

R

V

G

L

E

L

L

-

L

-

F

-

G

-

T

P

V

K

D

D

T

Y

W

I

L

S

I

W

W

R

K

M

M

T

T

G

Q

-

G

-

R

V

V

F

H

A

V

T

T

D

D

V

Y

S

T

D

N

A

A

S

S

G

R

M

T

L

M

L

L

L

F

D

N

V

I

V

H

N

T

R

L

K

D

W

W

S

D

S

D

E

K

M

M

L

L

A

E

A

V

L

L

E

D

I

I

P

P

A

R

A

E

W

M

L

L

A

P

E

E

V

V

F

R

E

R

S

S

P

S

D

E

V

V

V

Y

G

G

R

Q

V

T

S

N

A

I

R

-

G

-

A

-

A

-

E

-

T

-

G

-

L

-

P

-

E

-

G

-

I

I

P

P

V

I

V

S

A

G

G

I

A

A

G

G

D
|
D

N

Q

A

Q

A

A

G

A

A

L

V

F

G

G

N

Q

G

L

I

C

I

V

S

K

P

E

G

G

M

M

A

A

T

K

A

N

S

T

L

Y

G
|
G

T
|
T

S

G

G

C

V
x
F

V

M

F

L

T

M

P

N

S

T

Q

G

T

E

P

K

A

A

V

V

D

K

R

S

A

E

G

N

R

G

L

L

H

L

T

T

F

T

C

I

H

A

A

C

V

G

P

P

G

T

-

G

-

E

-

V

Q

N

W

Y

H

A

L

L

M

E

G

G

V

A

T

V

M

F

A

M

A

A

G
|
G

G

A

S

S

L
x
I

R

Q

W

W

Y

L

R

R

D

D

T

-

F

-

A

-

G

-

E

E

E

M

R

K

A

L

V

I

A

-

Q

-

L

-

T

-

G

-

R

N

D

D

P
x
A

Y

Y

E

D

L

-

M

-

S

S

D

E

E

Y

A

F

A

A

L

T

I

K

E

V

P

Q

G

N

A

T

D

N

G

G

L

V

I

Y

F

V

L

V

P

P

Y

A

L

F

S

T

G

G

E

L

R

G

T

A

P

P

H

Y

A

W

D

D

P

P

L

Y

A

A

R

R

G

G

V

A

F

I

V

F

G

G

M

L

N

T

L

R

K

G

H

V

T

N

K

A

G

N

H

H

F

I

V

I

R

R

S

A

I

T

M

L

E

E

G

S

V

I

A

A

F

Y

S

Q

L

T

K

R

D

D

T

V

L

L

E

E

I

A

M

M

-

Q

S

A

G

D

L

S

G

G

I

I

A

R

L

L

N

H

D

A

L

L

R

R

I

V

T

D

G

G

G
|
G

G
x
A

G

V

R

A

S
x
N

A

N

V

F

W

L

R

M

Q

Q

I

F

L

Q

A

S

D

D

V

I

F

L

N

G

M

T

P

R

L

V

Q

E

V

R

M

P

E

E

N

V

S

R

D

E

G

V

P

T

A

A

Y

L

G

G

A

A

A

A

I

Y

L

L

G

A

A

G

V

L

G

A

A

V

G

G

C

F

W

W

Q

Q

S

N

V

L

T

D

E

E

A

L

V

Q

A

E

A

K

T

A

C

V

S

I

R

E

Q

R

Q

E

L

F

Q

R

E

P

V

G

V

I

P

E

I

T

P

T

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E

N

R

A

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A

Y

R

R

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Y

binding adenosine-5'-diphosphate: R14 (≠ K16), G256 (= G261), T257 (= T262), G300 (= G302), I303 (≠ L305), A316 (≠ P327), G401 (= G413), A402 (≠ G414), N405 (≠ S417)
binding glycerol: R80 (≠ M81), E81 (≠ H82), W100 (= W100), Y132 (≠ A129), D235 (= D240), F260 (≠ V265)

6udeB Crystal structure of glycerol kinase from elizabethkingia anophelis nuhp1 in complex with adp and glycerol
29% identity, 90% coverage: 2:461/510 of query aligns to 2:455/495 of 6udeB

query
sites
6udeB

Q

E

S

K

L

L

F

I

L

L

G

A

I

L

D

D

I

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G
|
G

T
|
T

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|
T

G
x
S

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x
R

A

A

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I

V

L

M

F

D

N

E

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G

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E

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I

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E

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Y

F

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E

L

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H

I

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F

P

P

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T

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P

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G

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W

A

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Y

W

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C

I

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S

A

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I

A

A

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E

I

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I

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A

K

K

-

A

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G

I

I

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G

G

L

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E

I

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Q

A

G

A

I

I

G

G

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I

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G

N

Q

Q

M

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E

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I

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E

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A

A

I

I

L

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C

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Y

C

C

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-

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G

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I

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-

D

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A

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Y

F

F

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S

A

A

P

T

K

K

I

L

L

K

W

W

-

I

L

L

R

D

N

N

H

V

E

E

P

G

E

A

N

R

Y

E

K

K

R

A

V

E

A

A

K

G

V

D

L

L

L

C

-

F

-

G

-

T

P

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D

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Y

W

I

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I

W

W

R

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M

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-

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-

G

G

K

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F

A

I

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D

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S

S

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N

A

A

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R

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M

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M

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W

W

S

D

S

D

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D

M

L

L

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K

A

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I

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A

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S

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S

A

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A

K

E

-

T

-

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L

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-

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-

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-

I

I

P

P

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I

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A

A

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G

I

A

A

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G

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D

N

Q

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-

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-

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D

D

T

I

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V

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K

I

A

M

M

S

E

G

A

L

D

G

A

-

E

I

T

A

Q

L

S

N

T

D

E

L

L

R

R

I

V

T

D

G
|
G

G
|
G

G
x
A

G

S

R

A

S

S

A

N

V

L

W

L

R

M

Q

Q

I

I

L

Q

A

S

D

D

V

L

F

F

N

G

M

F

P

K

L

I

Q

I

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M

P

E

K

N

T

S

L

D

E

G

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P

T

A

A

Y

L

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G

A

A

A

A

I

Y

L

L

G

A

A

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V

L

G

A

A

V

G

G

C

F

W

W

Q

E

S

S

V

I

T

D

E

E

binding adenosine-5'-diphosphate: R16 (≠ K16), G262 (= G261), T263 (= T262), G306 (= G302), I309 (≠ L305), S323 (= S332), G406 (= G412), G407 (= G413), A408 (≠ G414)
binding magnesium ion: G11 (= G11), T12 (= T12), T13 (= T13), S14 (≠ G14)

O34153 Glycerol kinase; ATP:glycerol 3-phosphotransferase; Glycerokinase; GK; EC 2.7.1.30 from Enterococcus casseliflavus (Enterococcus flavescens) (see 3 papers)
27% identity, 95% coverage: 2:486/510 of query aligns to 4:485/506 of O34153

query
sites
O34153

Q

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x
R

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x
E

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V

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L

W

D

D

-

K

A

T

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T

G

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N

A

A

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A

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Q

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-

L

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E

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I

I

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H

L

E

V

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N

G

P

L

A

V

L

I

-

D

A

A

G
x
Y

F

F

T

S

A

A

P

T

K

K

I

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R

W

W

-

L

L

L

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D

N

N

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I

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E

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E

A

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Y

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A

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L

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-

F

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W

W

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K

M

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T

T

G

D

-

G

-

Q

V

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F

H

A

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T

D

D

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Y

S

S

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N

A

A

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M

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L

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A

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x
H

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A

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A

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|
D

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x
Q

A

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A

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E

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E

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A

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A

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Q

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K

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T

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L

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E

D

I

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M

M

-

K

-

K

-

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S

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I

I

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P

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N

-

D

-

L

L

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A

G

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A

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W

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A

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L

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I

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D

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Q

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M

A

E

A

N

N

S

L

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E

G

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P

T

A

A

Y

L

G

G

A

A

A

A

I

Y

L

L

G

A

A

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L

G

A

A

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G

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F

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W

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V

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E

E

A

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A

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A

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T

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C

E

S

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L

F

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E

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A

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E

N

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A

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A

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R

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Y

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Q

E

R84 (≠ M81) binding glycerol
E85 (≠ H82) binding glycerol
Y136 (≠ G132) binding glycerol
H232 (≠ G222) modified: Phosphohistidine; by HPr; mutation to A: Loss of phosphorylation, no effect on activity.; mutation to E: Loss of phosphorylation, 2.5-fold reduced activity.; mutation to R: Loss of phosphorylation, 3.4-fold increased activity.
D246 (= D240) binding glycerol
Q247 (≠ N241) binding glycerol

Query Sequence

>WP_008412468.1 NCBI__GCF_000315365.1:WP_008412468.1
MQSLFLGIDIGTTGVKALVMDEQGRVVAQATREYPLHVPRPGWAEQNPEDWYKAACGAIA
TIISKGISPGQIQGIGLTGQMHGSVFLDAQGKVIREAILWCDQRTADECRQITDLVGESK
LIELVSNPALAGFTAPKILWLRNHEPENYKRVAKVLLPKDYISWRMTGVFATDVSDASGM
LLLDVVNRKWSSEMLAALEIPAAWLAEVFESPDVVGRVSARGAAETGLPEGIPVVAGAGD
NAAGAVGNGIISPGMATASLGTSGVVFTPSQTPAVDRAGRLHTFCHAVPGQWHLMGVTMA
AGGSLRWYRDTFAGEERAVAQLTGRDPYELMSDEAALIEPGADGLIFLPYLSGERTPHAD
PLARGVFVGMNLKHTKGHFVRSIMEGVAFSLKDTLEIMSGLGIALNDLRITGGGGRSAVW
RQILADVFNMPLQVMENSDGPAYGAAILGAVGAGCWQSVTEAVAATCSRQQLQEVVPIPQ
NAARYQKIYQIYIDTYPPLQPLFKRLSEVN

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory