SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_008482959.1 NCBI__GCF_000299915.1:WP_008482959.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5xu1B Structure of a non-canonical abc transporter from streptococcus pneumoniae r6 (see paper)
52% identity, 94% coverage: 4:225/235 of query aligns to 3:224/226 of 5xu1B

query
sites
5xu1B

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binding magnesium ion: G45 (= G46), K46 (= K47), S47 (= S48)

1f3oA Crystal structure of mj0796 atp-binding cassette (see paper)
47% identity, 96% coverage: 4:229/235 of query aligns to 1:231/232 of 1f3oA

query
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1f3oA

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binding adenosine-5'-diphosphate: Y11 (≠ F14), G41 (= G44), G43 (= G46), K44 (= K47), S45 (= S48), T46 (= T49)

1l2tA Dimeric structure of mj0796, a bacterial abc transporter cassette (see paper)
47% identity, 94% coverage: 4:225/235 of query aligns to 1:227/230 of 1l2tA

query
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1l2tA

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binding adenosine-5'-triphosphate: Y11 (≠ F14), S40 (= S43), G41 (= G44), S42 (= S45), G43 (= G46), K44 (= K47), S45 (= S48), T46 (= T49), F138 (vs. gap), Q145 (= Q144), S147 (= S146), G149 (= G148), Q150 (= Q149), H204 (= H202)

5lj7A Structure of aggregatibacter actinomycetemcomitans macb bound to atp (p21) (see paper)
44% identity, 95% coverage: 3:225/235 of query aligns to 2:224/592 of 5lj7A

query
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5lj7A

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binding adenosine-5'-triphosphate: V22 (≠ A23), S42 (= S43), G43 (= G44), G45 (= G46), K46 (= K47), S47 (= S48), T48 (= T49), Q92 (= Q93), K136 (≠ R137), Q143 (= Q144), S145 (= S146), G147 (= G148), Q148 (= Q149)
binding magnesium ion: S47 (= S48), Q92 (= Q93)

5lilA Structure of aggregatibacter actinomycetemcomitans macb bound to atpys (p21) (see paper)
44% identity, 95% coverage: 3:225/235 of query aligns to 2:224/615 of 5lilA

query
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5lilA

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binding adenosine-5'-triphosphate: F13 (= F14), V22 (≠ A23), S42 (= S43), G43 (= G44), G45 (= G46), K46 (= K47), S47 (= S48), T48 (= T49), Q92 (= Q93), K136 (≠ R137), Q143 (= Q144), S145 (= S146), G147 (= G148), Q148 (= Q149)
binding magnesium ion: S47 (= S48), Q92 (= Q93)

P75831 Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
46% identity, 95% coverage: 1:224/235 of query aligns to 1:224/648 of P75831

query
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P75831

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C

L

L

D

D

V

K

P

A

S

T

E

S

G

G

H

T

Y

Y

W

R

L

V

A

A

G

G

E

Q

D

D

V

V

A

A

T

T

L

L

P

D

Q

A

S

D

Q

A

L

L

A

A

R

Q

V

L

R

R

N

R

H

E

Q

H

I

F

G

G

F

F

I

I

F

F

Q

Q

S

R

F

Y

N

H

L

L

L

L

P

S

R

H

A

L

S

T

A

A

L

E

D

Q

N

N

V

V

A

E

Q

V

P

P

L

A

V

V

Y

Y

Q

A

G

G

F

L

S

E

L

R

K

K

E

Q

R

R

R

L

Q

L

R

R

A

A

L

Q

E

E

A

L

L

L

A

Q

K

R

V

L

G

G

L

L

A

E

D

D

R

R

A

T

N

E

H

Y

R

Y

P

P

N

A

Q

Q

L

L

S

S

G

G

G

G

Q

Q

R

Q

Q

Q

R

R

V

V

A

S

I

I

A

A

R

R

A

A

L

L

V

M

T

N

R

G

P

G

S

Q

I

V

L

I

L

L

A

A

D
|
D

E

E

P

P

T

T

G

G

N

A

L

L

D

D

S

S

Q

H

T

S

T

G

R

E

D

E

I

V

M

M

A

A

L

I

F

L

D

H

E

Q

L

L

H

R

G

D

E

R

G

G

Q

H

T

T

I

V

V

I

V

I

V

V

T

T

H

H

E

D

Q

P

D

Q

I

V

A

A

N

A

H

Q

C

A

R

E

R

R

V

V

V

I

R

E

V

I

M

R

D

D

G

G

V

E

I

I

T

V

S

R

D

N

K47 (= K47) mutation to L: Lack of activity.
D169 (= D169) mutation to N: Lack of activity.

5ws4A Crystal structure of tripartite-type abc transporter macb from acinetobacter baumannii (see paper)
48% identity, 89% coverage: 17:224/235 of query aligns to 16:224/650 of 5ws4A

query
sites
5ws4A

A

A

G

G

E

E

P

S

-

T

F

I

W

Q

A

I

L

L

R

K

G

G

I

I

D

D

L

L

T

T

I

I

G

Y

R

E

N

G

D

E

Y

L

L

V

A

A

I

I

I

V

G

G

P

Q

S
|
S

G
|
G

S

S

G

G

K

K

S
|
S

T

T

L

L

L

M

N

N

I

I

L

L

G

G

C

C

L

L

D

D

V

R

P

P

S

T

E

S

G

G

H

S

Y

Y

W

K

L

V

A

N

G

G

E

Q

D

E

V

T

A

G

T

K

L

L

P

E

Q

P

S

D

Q

Q

L

L

A

A

R

Q

V

L

R

R

N

R

H

E

Q

Y

I

F

G

G

F

F

I

I

F

F

Q

Q

S

R

F

Y

N

H

L

L

L

L

P

G

R

D

A

L

S

S

A

A

L

E

D

G

N

N

V

V

A

E

Q

V

P

P

L

A

V

V

Y

Y

Q

A

G

G

F

V

S

T

L

P

K

A

E

D

R

R

R

K

Q

Q

R

R

A

A

L

T

E

A

A

L

L

L

A

T

K

E

V

L

G

G

L

L

A

G

D

T

R

K

A

T

N

Q

H

N

R

R

P

P

N

S

Q

Q

L

L

S

S

G

G

G

G

Q

Q

R

Q

Q

Q

R

R

V

V

A

S

I

I

A

A

R

R

A

A

L

L

V

M

T

N

R

G

P

G

S

D

I

V

L

I

L

L

A

A

D

D

E

E

P

P

T

T

G

G

N

A

L

L

D

D

S

S

Q

H

T

S

T

G

R

V

D

E

I

V

M

M

A

R

L

I

F

L

D

R

E

E

L

L

H

N

G

A

E

A

G

G

Q

H

T

T

I

I

V

I

V

L

V

V

T

T

H

H

E

D

Q

M

D

Q

I

V

A

A

N

K

H

N

C

A

R

T

R

R

V

I

V

I

R

E

V

I

M

S

D

D

G

G

V

E

I

I

T

I

S

S

D

D

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: S43 (= S43), G44 (= G44), S48 (= S48)

2pclA Crystal structure of abc transporter with complex (aq_297) from aquifex aeolicus vf5
43% identity, 94% coverage: 3:224/235 of query aligns to 2:219/223 of 2pclA

query
sites
2pclA

D

E

V

I

I

L

C

R

L

A

E

E

A

N

I

I

K

K

K

K

A

-

F

-

V

V

M

I

A

R

G

G

E

-

P

-

F

Y

W

E

A

I

L

L

R

K

G

G

I

I

D

S

L

L

T

S

I

V

G

K

R

K

N

G

D

E

Y

F

L

V

A

S

I

I

I

I

G

G

P

A

S

S

G

G

S

S

G

G

K

K

S

S

T

T

L

L

L

L

N

Y

I

I

L

L

G

G

C

L

L

L

D

D

V

A

P

P

S

T

E

E

G

G

H

K

Y

V

W

F

L

L

A

E

G

G

E

K

D

E

V

V

A

D

T

Y

L

T

P

N

Q

E

S

K

Q

E

L

L

A

S

R

L

V

L

R

R

N

N

H

R

Q

K

I

L

G

G

F

F

I

V

F

F

Q

Q

S

F

F

H

N

Y

L

L

L

I

P

P

R

E

A

L

S

T

A

A

L

L

D

E

N

N

V

V

A

I

Q

V

P

P

L

M

V

L

Y

K

Q

M

G

G

F

K

S

P

L

K

K

K

E

E

R

A

R

K

Q

E

R

R

A

G

L

E

E

Y

A

L

L

L

A

S

K

E

V

L

G

G

L

L

A

G

D

D

R

K

A

L

N

S

H

R

R

K

P

P

N

Y

Q

E

L

L

S

S

G

G

G

G

Q

E

R

Q

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

V

A

T

N

R

E

P

P

S

I

I

L

L

L

L

F

A

A

D

D

E
|
E

P
|
P

T
|
T

G

G

N

N

L

L

D

D

S

S

Q

A

T

N

T

T

R

K

D

R

I

V

M

M

A

D

L

I

F

F

D

L

E

K

L

I

H

N

G

E

E

G

G

G

Q

T

T

S

I

I

V

V

V

M

V

V

T
|
T

H

H

E

E

Q

R

D

E

I

L

A

A

N

E

H

L

C

T

R

H

R

R

V

T

V

L

R

E

V

M

M

K

D

D

G

G

V

K

I

V

T

V

S

G

D

E

binding magnesium ion: E165 (= E170), P166 (= P171), T167 (= T172), T196 (= T201)

P75957 Lipoprotein-releasing system ATP-binding protein LolD; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
46% identity, 89% coverage: 24:231/235 of query aligns to 25:233/233 of P75957

query
sites
P75957

L

L

R

H

G

N

I

V

D

S

L

F

T

S

I

V

G

G

R

E

N

G

D

E

Y

M

L

M

A

A

I

I

I

V

G
|
G

P

S

S

S

G

G

S

S

G

G

K

K

S

S

T

T

L

L

L

L

N

H

I

L

L

L

G

G

C

G

L

L

D

D

V

T

P

P

S

T

E

S

G

G

H

D

Y

V

W

I

L

F

A

N

G

G

E

Q

D

P

V

M

A

S

T

K

L

L

P

S

Q

S

S

A

Q

A

L

K

A

A

R

E

V

L

R

R

N

N

H

Q

Q

K

I

L

G

G

F

F

I

I

F

Y

Q

Q

S

F

F

H

N

H

L

L

L

L

P

P

R

D

A

F

S

T

A

A

L

L

D

E

N

N

V

V

A

A

Q

M

P

P

L

L

V

L

Y

I

Q

G

G

K

F

K

S

K

L

P

K

A

E

E

R

I

R

N

Q

S

R

R

A

A

L

L

E

E

A

M

L

L

A

K

K

A

V

V

G

G

L

L

A

D

D

H

R

R

A

A

N

N

H

H

R

R

P

P

N

S

Q

E

L

L

S

S

G

G

G

G

Q

E

R

R

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

V

V

T

N

R

N

P

P

S

R

I

L

L

V

L

L

A

A

D

D

E

E

P

P

T

T

G

G

N

N

L

L

D

D

S

A

Q

R

T

N

T

A

R

D

D

S

I

I

M

F

A

Q

L

L

F

L

D

G

E

E

L

L

H

N

G

R

-

L

E

Q

G

G

Q

T

T

A

I

F

V

L

V

V

V

V

T

T

H

H

E

D

Q

L

D

Q

I

L

A

A

N

K

H

R

C

M

R

S

R

R

V

Q

V

L

R

E

V

M

M

R

D

D

G

G

V

R

I

L

T

T

S

A

D

E

S

L

L

S

V

L

A

M

G

G

Q

A

E

E

G42 (= G41) mutation to D: Loss of lipoprotein release when overexpressed.

7mdyC Lolcde nucleotide-bound
47% identity, 86% coverage: 24:224/235 of query aligns to 22:223/226 of 7mdyC

query
sites
7mdyC

L

L

R

H

G

N

I

V

D

S

L

F

T

S

I

V

G

G

R

E

N

G

D

E

Y

M

L

M

A

A

I

I

I

V

G

G

P

S

S

S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

L

N

H

I

L

L

L

G

G

C

G

L

L

D

D

V

T

P

P

S

T

E

S

G

G

H

D

Y

V

W

I

L

F

A

N

G

G

E

Q

D

P

V

M

A

S

T

K

L

L

P

S

Q

S

S

A

Q

A

L

K

A

A

R

E

V

L

R

R

N

N

H

Q

Q

K

I

L

G

G

F

F

I

I

F

Y

Q
|
Q

S

F

F

H

N

H

L

L

L

L

P

P

R

D

A

F

S

T

A

A

L

L

D

E

N

N

V

V

A

A

Q

M

P

P

L

L

V

L

Y

I

Q

G

G

K

F

K

S

K

L

P

K

A

E

E

R

I

R

N

Q

S

R

R

A

A

L

L

E

E

A

M

L

L

A

K

K

A

V

V

G

G

L

L

A

D

D

H

R

R

A

A

N

N

H
|
H

R

R

P

P

N

S

Q
x
E

L

L

S
|
S

G
|
G

G
|
G

Q

E

R

R

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

V

V

T

N

R

N

P

P

S

R

I

L

L

V

L

L

A

A

D

D

E
|
E

P

P

T

T

G

G

N
|
N

L

L

D

D

S

A

Q

R

T

N

T

A

R

D

D

S

I

I

M

F

A

Q

L

L

F

L

D

G

E

E

L

L

H

N

G

R

-

L

E

Q

G

G

Q

T

T

A

I

F

V

L

V

V

V

V

T

T

H
|
H

E

D

Q

L

D

Q

I

L

A

A

N

K

H

R

C

M

R

S

R

R

V

Q

V

L

R

E

V

M

M

R

D

D

G

G

V

R

I

L

T

T

S

A

D

E

binding adp orthovanadate: G42 (= G44), S43 (= S45), G44 (= G46), K45 (= K47), S46 (= S48), T47 (= T49), Q91 (= Q93), H138 (= H140), E142 (≠ Q144), S144 (= S146), G145 (= G147), G146 (= G148), E168 (= E170), N172 (= N174), H201 (= H202)
binding magnesium ion: S46 (= S48), Q91 (= Q93)

Sites not aligning to the query:

binding adp orthovanadate: 12

7arlD Lolcde in complex with lipoprotein and adp (see paper)
47% identity, 85% coverage: 24:223/235 of query aligns to 22:222/222 of 7arlD

query
sites
7arlD

L

L

R

H

G

N

I

V

D

S

L

F

T

S

I

V

G

G

R

E

N

G

D

E

Y

M

L

M

A

A

I

I

I

V

G

G

P

S

S

S

G
|
G

S

S

G
|
G

K
|
K

S
|
S

T

T

L

L

L

L

N

H

I

L

L

L

G

G

C

G

L

L

D

D

V

T

P

P

S

T

E

S

G

G

H

D

Y

V

W

I

L

F

A

N

G

G

E

Q

D

P

V

M

A

S

T

K

L

L

P

S

Q

S

S

A

Q

A

L

K

A

A

R

E

V

L

R

R

N

N

H

Q

Q

K

I

L

G

G

F

F

I

I

F

Y

Q

Q

S

F

F

H

N

H

L

L

L

L

P

P

R

D

A

F

S

T

A

A

L

L

D

E

N

N

V

V

A

A

Q

M

P

P

L

L

V

L

Y

I

Q

G

G

K

F

K

S

K

L

P

K

A

E

E

R

I

R

N

Q

S

R

R

A

A

L

L

E

E

A

M

L

L

A

K

K

A

V

V

G

G

L

L

A

D

D

H

R

R

A

A

N

N

H

H

R

R

P

P

N

S

Q

E

L

L

S

S

G

G

G

G

Q

E

R

R

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

V

V

T

N

R

N

P

P

S

R

I

L

L

V

L

L

A

A

D

D

E

E

P

P

T

T

G

G

N

N

L

L

D

D

S

A

Q

R

T

N

T

A

R

D

D

S

I

I

M

F

A

Q

L

L

F

L

D

G

E

E

L

L

H

N

G

R

-

L

E

Q

G

G

Q

T

T

A

I

F

V

L

V

V

V

V

T

T

H

H

E

D

Q

L

D

Q

I

L

A

A

N

K

H

R

C

M

R

S

R

R

V

Q

V

L

R

E

V

M

M

R

D

D

G

G

V

R

I

L

T

T

S

A

binding adenosine-5'-diphosphate: G42 (= G44), G44 (= G46), K45 (= K47), S46 (= S48)

7v8iD Lolcd(e171q)e with bound amppnp in nanodiscs (see paper)
46% identity, 86% coverage: 24:224/235 of query aligns to 24:225/229 of 7v8iD

query
sites
7v8iD

L

L

R

H

G

N

I

V

D

S

L

F

T

S

I

V

G

G

R

E

N

G

D

E

Y

M

L

M

A

A

I

I

I

V

G

G

P

S

S
|
S

G
|
G

S

S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

L

N

H

I

L

L

L

G

G

C

G

L

L

D

D

V

T

P

P

S

T

E

S

G

G

H

D

Y

V

W

I

L

F

A

N

G

G

E

Q

D

P

V

M

A

S

T

K

L

L

P

S

Q

S

S

A

Q

A

L

K

A

A

R

E

V

L

R

R

N

N

H

Q

Q

K

I

L

G

G

F

F

I

I

F

Y

Q
|
Q

S

F

F

H

N

H

L

L

L

L

P

P

R

D

A

F

S

T

A

A

L

L

D

E

N

N

V

V

A

A

Q

M

P

P

L

L

V

L

Y

I

Q

G

G

K

F

K

S

K

L

P

K

A

E

E

R

I

R

N

Q

S

R

R

A

A

L

L

E

E

A

M

L

L

A

K

K

A

V

V

G

G

L

L

A

D

D

H

R
|
R

A

A

N

N

H
|
H

R

R

P

P

N

S

Q
x
E

L

L

S
|
S

G

G

G
|
G

Q
x
E

R

R

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

V

V

T

N

R

N

P

P

S

R

I

L

L

V

L

L

A

A

D

D

E

Q

P

P

T

T

G

G

N

N

L

L

D

D

S

A

Q

R

T

N

T

A

R

D

D

S

I

I

M

F

A

Q

L

L

F

L

D

G

E

E

L

L

H

N

G

R

-

L

E

Q

G

G

Q

T

T

A

I

F

V

L

V

V

V

V

T

T

H
|
H

E

D

Q

L

D

Q

I

L

A

A

N

K

H

R

C

M

R

S

R

R

V

Q

V

L

R

E

V

M

M

R

D

D

G

G

V

R

I

L

T

T

S

A

D

E

binding phosphoaminophosphonic acid-adenylate ester: S43 (= S43), G44 (= G44), G46 (= G46), K47 (= K47), S48 (= S48), T49 (= T49), Q93 (= Q93), R137 (= R137), H140 (= H140), E144 (≠ Q144), S146 (= S146), G148 (= G148), E149 (≠ Q149), H203 (= H202)
binding magnesium ion: S48 (= S48), Q93 (= Q93)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 23

8g4cB Bceabs atpgs high res tm (see paper)
37% identity, 97% coverage: 5:231/235 of query aligns to 2:231/248 of 8g4cB

query
sites
8g4cB

I

V

C

I

L

L

E

E

A

A

-

N

-

K

I

I

K

R

K

K

A

S

F
x
Y

V

G

M

N

A

K

G

L

E

N

P

K

F

Q

W

E

A

V

L

L

R

K

G

G

I

I

D

D

L

I

T

H

I

I

G

E

R

K

N

G

D

E

Y

F

L

V

A

S

I

I

I

M

G

G

P

A

S
|
S

G

G

S
|
S

G

G

K

K

S
x
T

T

T

L

L

L

L

N

N

I

V

L

L

G

S

C

S

L

I

D

D

V

Q

P

V

S

S

E

H

G

G

H

T

Y

I

W

H

L

I

A

N

G

G

E

N

D

D

V

M

A

T

T

A

L

M

P

K

Q

E

S

K

Q

Q

L

L

A

A

R

E

V

F

R

R

N

K

H

Q

Q

H

I

L

G

G

F

F

I

I

F

F

Q

Q

S

D

F

Y

N

N

L

L

L

L

P

D

R

T

A

L

S

T

A

V

L

K

D

E

N

N

V

I

A

L

Q

L

P

P

L

L

V

S

Y

I

Q

T

G

K

F

L

S

S

L

K

K

K

E

E

R

A

R

N

Q

R

R

K

A

F

L

E

E

E

A

V

L

A

A

K

K

E

V

L

G

G

L

I

A

Y

D

E

R

L

A

R

N

D

H

K

R

Y

P

P

N

N

Q

E

L

I

S

S

G

G

G

G

Q

Q

R

K

Q

Q

R

R

V

T

A

S

I

A

A

G

R

R

A

A

L

F

V

I

T

H

R

D

P

P

S

S

I

I

L

I

L

F

A

A

D
|
D

E

E

P

P

T

T

G

G

N

A

L

L

D

D

S

S

Q

K

T

S

T

A

R

S

D

D

I

L

M

L

A

N

L

K

F

L

D

S

E

Q

L

L

H

N

G

Q

E

K

-

R

G

N

Q

A

T

T

I

I

V

I

V

M

V

V

T

T

H

H

E

D

Q

P

D

V

I

A

A

A

N

S

H

Y

C

C

R

G

R

R

V

V

V

I

R

F

V

I

M

K

D

D

G

G

V

Q

I

M

T

Y

S

T

D

Q

S

L

L

N

V

K

A

G

G

G

Q

Q

E

D

binding phosphothiophosphoric acid-adenylate ester: Y13 (≠ F14), S42 (= S43), S44 (= S45), T47 (≠ S48), D168 (= D169)

7tchB Bceab e169q variant atp-bound conformation (see paper)
36% identity, 97% coverage: 5:231/235 of query aligns to 1:230/245 of 7tchB

query
sites
7tchB

I

V

C

I

L

L

E

E

A

A

-

N

-

K

I

I

K

R

K

K

A

S

F
x
Y

V

G

M

N

A

K

G

L

E

N

P

K

F
x
Q

W

E

A
x
V

L

L

R

K

G

G

I

I

D

D

L

I

T

H

I

I

G

E

R

K

N

G

D

E

Y

F

L

V

A

S

I

I

I

M

G

G

P

A

S
|
S

G
|
G

S
|
S

G

G

K
|
K

S
x
T

T

T

L

L

L

L

N

N

I

V

L

L

G

S

C

S

L

I

D

D

V

Q

P

V

S

S

E

H

G

G

H

T

Y

I

W

H

L

I

A

N

G

G

E

N

D

D

V

M

A

T

T

A

L

M

P

K

Q

E

S

K

Q

Q

L

L

A

A

R

E

V

F

R

R

N

K

H

Q

Q

H

I

L

G

G

F

F

I

I

F

F

Q

Q

S

D

F

Y

N

N

L

L

L

L

P

D

R

T

A

L

S

T

A

V

L

K

D

E

N

N

V

I

A

L

Q

L

P

P

L

L

V

S

Y

I

Q

T

G

K

F

L

S

S

L

K

K

K

E

E

R

A

R

N

Q

R

R

K

A

F

L

E

E

E

A

V

L

A

A

K

K

E

V

L

G

G

L

I

A

Y

D

E

R

L

A

R

N

D

H

K

R

Y

P

P

N

N

Q
x
E

L

I

S

S

G
|
G

G
|
G

Q

Q

R

K

Q

Q

R

R

V

T

A

S

I

A

A

G

R

R

A

A

L

F

V

I

T

H

R

D

P

P

S

S

I

I

L

I

L

F

A

A

D

D

E

Q

P

P

T

T

G

G

N

A

L

L

D

D

S

S

Q

K

T

S

T

A

R

S

D

D

I

L

M

L

A

N

L

K

F

L

D

S

E

Q

L

L

H

N

G

Q

E

K

-

R

G

N

Q

A

T

T

I

I

V

I

V

M

V

V

T

T

H

H

E

D

Q

P

D

V

I

A

A

A

N

S

H

Y

C

C

R

G

R

R

V

V

V

I

R

F

V

I

M

K

D

D

G

G

V

Q

I

M

T

Y

S

T

D

Q

S

L

L

N

V

K

A

G

G

G

Q

Q

E

D

binding adenosine-5'-triphosphate: Y12 (≠ F14), Q19 (≠ F21), V21 (≠ A23), S41 (= S43), G42 (= G44), S43 (= S45), K45 (= K47), T46 (≠ S48), E142 (≠ Q144), G145 (= G147), G146 (= G148)

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
40% identity, 86% coverage: 23:224/235 of query aligns to 16:217/240 of 4ymuJ

query
sites
4ymuJ

A
x
V

L

L

R

K

G

G

I

V

D

T

L

L

T

K

I

V

G

N

R

K

N

G

D

E

Y

V

L

V

A

V

I

I

I

I

G

G

P

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

L

N

R

I

C

L

I

G

N

C

L

L

L

D

E

V

E

P

P

S

T

E

K

G

G

H

E

Y

V

W

F

L

I

A

D

G

G

E

V

D

K

V

I

A

N

T

N

L

-

P

G

Q

K

S

V

Q

N

L

I

A

N

R

K

V

V

R

R

N

-

H

Q

Q

K

I

V

G

G

F

M

I

V

F

F

Q

Q

S

H

F

F

N

N

L

L

L

F

P

P

R

H

A

L

S

T

A

A

L

I

D

E

N

N

V

I

A

T

-

L

Q

A

P

P

L

V

V

K

Y

V

Q

K

G

K

F

M

S

N

L

K

K

K

E

E

R

A

R

E

Q

E

R

L

A

A

L

V

E

D

A

L

L

L

A

A

K

K

V

V

G

G

L

L

A

L

D

D

R

K

A

K

N

D

H

Q

R

Y

P

P

N

I

Q

K

L

L

S

S

G

G

G

G

Q

Q

R

K

Q

Q

R

R

V

L

A

A

I

I

A

A

R

R

A

A

L

L

V

A

T

M

R

Q

P

P

S

E

I

V

L

M

L

L

A

F

D

D

E
|
E

P

P

T

T

G

S

N

A

L

L

D

D

S

P

Q

E

T

M

T

V

R

K

D

E

I

V

M

L

A

N

L

V

F

M

D

K

E

Q

L

L

H

A

G

N

E

E

G

G

Q

M

T

T

I

M

V

V

V

V

V

V

T

T

H
|
H

E

E

Q

M

D

G

I

F

A

A

N

R

H

E

C

V

-

G

R

D

R

R

V

V

V

I

R

F

V

M

M

D

D

D

G

G

V

V

I

I

T

V

S

E

D

E

binding adenosine-5'-triphosphate: V16 (≠ A23), S36 (= S43), G37 (= G44), S38 (= S45), G39 (= G46), K40 (= K47), S41 (= S48), T42 (= T49), E162 (= E170), H194 (= H202)
binding magnesium ion: S41 (= S48), E162 (= E170)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 11

8w6iD Cryo-em structure of escherichia coli str k12 ftsex complex with atp- gamma-s in peptidisc
41% identity, 92% coverage: 4:219/235 of query aligns to 1:213/219 of 8w6iD

query
sites
8w6iD

V

M

I

I

C

R

L

F

E

E

A

H

I

V

K

S

K

K

A

A

F
x
Y

V

L

M

G

A

G

G

R

E

Q

P

-

F

-

W

-

A

A

L

L

R

Q

G

G

I

V

D

T

L

F

T

H

I

M

G

Q

R

P

N

G

D

E

Y

M

L

A

A

F

I

L

I

T

G

G

P

H

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

L

N

K

I

L

L

I

G

C

C

G

L

I

D

E

V

R

P

P

S

S

E

A

G

G

H

K

Y

I

W

W

L

F

A

S

G

G

E

H

D

D

V

I

A

T

T

R

L

L

P

K

Q

N

S

R

Q

E

L

V

A

P

R

F

V

L

R

R

N

R

H

-

Q

Q

I

I

G

G

F

M

I

I

F

F

Q
|
Q

S

D

F

H

N

H

L

L

L

L

P

M

R

D

A

R

S

T

A

V

L

Y

D

D

N

N

V

V

A

A

Q

I

P

P

L

L

V

I

Y

I

Q

A

G

G

F

A

S

S

L

G

K

D

E

D

R

I

R

R

Q

R

R

R

A

V

L

S

E

A

A

A

L

L

A

D

K

K

V

V

G

G

L

L

A

L

D

D

R
x
K

A

A

N

K

H

N

R

F

P

P

N

I

Q
|
Q

L

L

S
|
S

G
|
G

G
|
G

Q
x
E

R

Q

Q

Q

R

R

V

V

A

G

I

I

A

A

R

R

A

A

L

V

V

V

T

N

R

K

P

P

S

A

I

V

L

L

L

L

A

A

D

D

E

E

P

P

T

T

G

G

N

N

L

L

D

D

S

D

Q

A

T

L

T

S

R

E

D

G

I

I

M

L

A

R

L

L

F

F

D

E

E

E

L

F

H

N

G

R

E

V

G

G

Q

V

T

T

I

V

V

L

V

M

V

A

T

T

H
|
H

E

D

Q

I

D

N

-

L

I

I

A

S

N

R

H

R

C

S

R

Y

R

R

V

M

V

L

R

T

V

L

M

S

D

D

G

G

binding phosphothiophosphoric acid-adenylate ester: Y11 (≠ F14), S37 (= S43), G38 (= G44), A39 (≠ S45), G40 (= G46), K41 (= K47), S42 (= S48), T43 (= T49), Q86 (= Q93), K130 (≠ R137), Q137 (= Q144), S139 (= S146), G140 (= G147), G141 (= G148), E142 (≠ Q149), H195 (= H202)

P0A9R7 Cell division ATP-binding protein FtsE from Escherichia coli (strain K12) (see paper)
41% identity, 92% coverage: 4:219/235 of query aligns to 1:213/222 of P0A9R7

query
sites
P0A9R7

V

M

I

I

C

R

L

F

E

E

A

H

I

V

K

S

K

K

A

A

F

Y

V

L

M

G

A

G

G

R

E

Q

P

-

F

-

W

-

A

A

L

L

R

Q

G

G

I

V

D

T

L

F

T

H

I

M

G

Q

R

P

N

G

D

E

Y

M

L

A

A

F

I

L

I

T

G

G

P

H

S

S

G

G

S

A

G

G

K
|
K

S

S

T

T

L

L

L

L

N

K

I

L

L

I

G
x
C

C

G

L

I

D

E

V

R

P

P

S

S

E

A

G

G

H

K

Y

I

W

W

L

F

A

S

G

G

E

H

D

D

V

I

A

T

T

R

L

L

P

K

Q

N

S

R

Q

E

L

V

A

P

R

F

V

L

R

R

N

R

H

-

Q

Q

I

I

G

G

F

M

I

I

F

F

Q

Q

S

D

F

H

N

H

L

L

L

L

P

M

R

D

A

R

S

T

A

V

L

Y

D

D

N

N

V

V

A

A

Q

I

P

P

L

L

V

I

Y

I

Q

A

G

G

F

A

S

S

L

G

K

D

E

D

R

I

R

R

Q

R

R

R

A

V

L

S

E

A

A

A

L

L

A

D

K

K

V

V

G

G

L

L

A

L

D

D

R

K

A

A

N

K

H

N

R

F

P

P

N

I

Q

Q

L

L

S

S

G

G

G

G

Q

E

R

Q

Q

Q

R

R

V

V

A

G

I

I

A

A

R

R

A

A

L

V

V

V

T

N

R

K

P

P

S

A

I

V

L

L

L

L

A

A

D

D

E

E

P

P

T

T

G

G

N

N

L

L

D

D

S

D

Q

A

T

L

T

S

R

E

D

G

I

I

M

L

A

R

L

L

F

F

D

E

E

E

L

F

H

N

G

R

E

V

G

G

Q

V

T

T

I

V

V

L

V

M

V

A

T

T

H

H

E

D

Q

I

D

N

-

L

I

I

A

S

N

R

H

R

C

S

R

Y

R

R

V

M

V

L

R

T

V

L

M

S

D

D

G

G

K41 (= K47) mutation to R: Does not bind ATP.
C49 (≠ G55) mutation to A: Prevents dimer formation. Does not alter ATP-binding.

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
39% identity, 96% coverage: 4:228/235 of query aligns to 1:226/343 of P30750

query
sites
P30750

V

M

I

I

C

K

L

L

E

S

A

N

I

I

K

T

K

K

A

V

F

F

V

H

M

Q

A

G

G

T

E

R

P

T

F

I

W

Q

A

A

L

L

R

N

G

N

I

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S

L

L

T

H

I

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G

P

R

A

N

G

D

Q

Y

I

L

Y

A

G

I

V

I

I

G

G

P

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

N

R

I

C

L

V

G

N

C

L

L

L

D

E

V

R

P

P

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T

E

E

G

G

H

S

Y

V

W

L

L

V

A

D

G

G

E

Q

D

E

V

L

A

T

T

T

L

L

P

S

Q

E

S

S

Q

E

L

L

A

T

R

K

V

A

R

R

N

-

H

R

Q

Q

I

I

G

G

F

M

I

I

F

F

Q

Q

S

H

F

F

N

N

L

L

L

L

P

S

R

S

A

R

S

T

A

V

L

F

D

G

N

N

V

V

A

A

Q

L

P

P

L

L

V

E

Y

L

Q

D

G

N

F

T

S

P

L

K

K

D

E

E

R

V

R

K

Q

R

R

R

A

V

L

T

E

E

A

L

L

L

A

S

K

L

V

V

G

G

L

L

A

G

D

D

R

K

A

H

N

D

H

S

R

Y

P

P

N

S

Q

N

L

L

S

S

G

G

G

G

Q

Q

R

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

V

A

T

S

R

N

P

P

S

K

I

V

L

L

L

L

A

C

D

D

E
|
E

P

A

T

T

G

S

N

A

L

L

D

D

S

P

Q

A

T

T

T

T

R

R

D

S

I

I

M

L

A

E

L

L

F

L

D

K

E

D

L

I

H

N

G

R

E

R

-

L

G

G

Q

L

T

T

I

I

V

L

V

L

V

I

T

T

H

H

E

E

Q

M

D

D

I

V

A

V

N

K

H

R

-

I

C

C

R

D

R

C

V

V

V

A

R

V

V

I

M

S

D

N

G

G

V

E

I

L

T

I

S

E

D

Q

S

D

L

T

V

V

A

S

40:46 (vs. 43:49, 86% identical) binding ATP
E166 (= E170) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding L-methionine
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding L-methionine

8hd0A Cell divisome spg hydrolysis machinery ftsex-envc
40% identity, 92% coverage: 4:219/235 of query aligns to 1:213/218 of 8hd0A

query
sites
8hd0A

V

M

I

I

C

R

L

F

E

E

A

H

I

V

K

S

K

K

A

A

F
x
Y

V

L

M

G

A

G

G
x
R

E

Q

P

-

F

-

W

-

A
|
A

L

L

R

Q

G

G

I

V

D

T

L

F

T

H

I

M

G

Q

R

P

N

G

D

E

Y

M

L

A

A

F

I

L

I

T

G

G

P

H

S

S

G

G

S

A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

L

N

K

I

L

L

I

G

C

C

G

L

I

D

E

V

R

P

P

S

S

E

A

G

G

H

K

Y

I

W

W

L

F

A

S

G

G

E

H

D

D

V

I

A

T

T

R

L

L

P

K

Q

N

S

R

Q

E

L

V

A

P

R

F

V

L

R

R

N

R

H

-

Q

Q

I

I

G

G

F

M

I

I

F

F

Q

Q

S

D

F

H

N

H

L

L

L

L

P

M

R

D

A

R

S

T

A

V

L

Y

D

D

N

N

V

V

A

A

Q

I

P

P

L

L

V

I

Y

I

Q

A

G

G

F

A

S

S

L

G

K

D

E

D

R

I

R

R

Q

R

R

R

A

V

L

S

E

A

A

A

L

L

A

D

K

K

V

V

G

G

L

L

A

L

D

D

R

K

A

A

N

K

H

N

R

F

P

P

N

I

Q

Q

L

L

S
|
S

G

G

G
|
G

Q

E

R

Q

Q

Q

R

R

V

V

A

G

I

I

A

A

R

R

A

A

L

V

V

V

T

N

R

K

P

P

S

A

I

V

L

L

L

L

A

A

D

D

E

Q

P

P

T

T

G

G

N

N

L

L

D

D

S

D

Q

A

T

L

T

S

R

E

D

G

I

I

M

L

A

R

L

L

F

F

D

E

E

E

L

F

H

N

G

R

E

V

G

G

Q

V

T

T

I

V

V

L

V

M

V

A

T

T

H

H

E

D

Q

I

D

N

-

L

I

I

A

S

N

R

H

R

C

S

R

Y

R

R

V

M

V

L

R

T

V

L

M

S

D

D

G

G

binding adenosine-5'-triphosphate: Y11 (≠ F14), R15 (≠ G18), A17 (= A23), G40 (= G46), K41 (= K47), S42 (= S48), T43 (= T49), S139 (= S146), G141 (= G148)

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
39% identity, 96% coverage: 4:228/235 of query aligns to 2:227/344 of 3tuzC

query
sites
3tuzC

V

M

I

I

C

K

L

L

E

S

A

N

I

I

K

T

K

K

A

V

F
|
F

V

H

M
x
Q

A

G

G

T

E

R

P

T

F

I

W

Q

A

A

L

L

R

N

G

N

I

V

D

S

L

L

T

H

I

V

G

P

R

A

N

G

D

Q

Y

I

L

Y

A

G

I

V

I

I

G

G

P

A

S

S

G
|
G

S

A

G
|
G

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|
K

S
|
S

T
|
T

L

L

L

I

N

R

I

C

L

V

G

N

C

L

L

L

D

E

V

R

P

P

S

T

E

E

G

G

H

S

Y

V

W

L

L

V

A

D

G

G

E

Q

D

E

V

L

A

T

T

T

L

L

P

S

Q

E

S

S

Q

E

L

L

A

T

R

K

V

A

R

R

N

R

H

-

Q

Q

I

I

G

G

F

M

I

I

F

F

Q

Q

S

H

F

F

N

N

L

L

L

L

P

S

R

S

A

R

S

T

A

V

L

F

D

G

N

N

V

V

A

A

Q

L

P

P

L

L

V

E

Y

L

Q

D

G

N

F

T

S

P

L

K

K

D

E

E

R

V

R

K

Q

R

R

R

A

V

L

T

E

E

A

L

L

L

A

S

K

L

V

V

G

G

L

L

A

G

D

D

R

K

A

H

N

D

H

S

R

Y

P

P

N

S

Q

N

L

L

S

S

G

G

G

G

Q

Q

R

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

V

A

T

S

R

N

P

P

S

K

I

V

L

L

L

L

A

C

D

D

E

Q

P

A

T

T

G

S

N

A

L

L

D

D

S

P

Q

A

T

T

T

T

R

R

D

S

I

I

M

L

A

E

L

L

F

L

D

K

E

D

L

I

H

N

G

R

E

R

-

L

G

G

Q

L

T

T

I

I

V

L

V

L

V

I

T

T

H

H

E

E

Q

M

D

D

I

V

A

V

N

K

H

R

-

I

C

C

R

D

R

C

V

V

V

A

R

V

V

I

M

S

D

N

G

G

V

E

I

L

T

I

S

E

D

Q

S

D

L

T

V

V

A

S

binding adenosine-5'-diphosphate: F12 (= F14), Q14 (≠ M16), G42 (= G44), G44 (= G46), K45 (= K47), S46 (= S48), T47 (= T49)

Sites not aligning to the query:

binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 313

Query Sequence

>WP_008482959.1 NCBI__GCF_000299915.1:WP_008482959.1
MTDVICLEAIKKAFVMAGEPFWALRGIDLTIGRNDYLAIIGPSGSGKSTLLNILGCLDVP
SEGHYWLAGEDVATLPQSQLARVRNHQIGFIFQSFNLLPRASALDNVAQPLVYQGFSLKE
RRQRALEALAKVGLADRANHRPNQLSGGQRQRVAIARALVTRPSILLADEPTGNLDSQTT
RDIMALFDELHGEGQTIVVVTHEQDIANHCRRVVRVMDGVITSDSLVAGQESRLV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory