SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_008503624.1 NCBI__GCF_000182725.1:WP_008503624.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
41% identity, 96% coverage: 2:244/254 of query aligns to 5:245/247 of 4jroC

query
sites
4jroC

G

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active site: G16 (= G13), S142 (= S141), Q152 (≠ L151), Y155 (= Y154), K159 (= K158)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G9), S14 (= S11), R15 (≠ T12), G16 (= G13), I17 (= I14), N35 (≠ S32), Y36 (= Y33), N37 (≠ R34), G38 (≠ H35), S39 (≠ D36), N63 (≠ D60), V64 (= V61), N90 (= N88), A91 (= A89), I93 (= I91), I113 (≠ T111), S142 (= S141), Y155 (= Y154), K159 (= K158), P185 (= P184), I188 (= I187), T190 (≠ N189)

3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
40% identity, 96% coverage: 1:245/254 of query aligns to 3:245/246 of 3osuA

query
sites
3osuA

M

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active site: G15 (= G13), S141 (= S141), Q151 (≠ L151), Y154 (= Y154), K158 (= K158)
binding magnesium ion: N89 (= N88), K158 (= K158)

3sj7A Structure of beta-ketoacetyl-coa reductase (fabg) from staphylococcus aureus complex with NADPH (see paper)
40% identity, 95% coverage: 5:245/254 of query aligns to 4:238/239 of 3sj7A

query
sites
3sj7A

A

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active site: G12 (= G13), S138 (= S141), Q148 (≠ L151), Y151 (= Y154), K155 (= K158)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G9), S10 (= S11), R11 (≠ T12), I13 (= I14), N31 (≠ S32), Y32 (= Y33), A33 (≠ R34), G34 (≠ H35), S35 (≠ D36), A58 (≠ C59), N59 (≠ D60), V60 (= V61), N86 (= N88), A87 (= A89), T109 (= T111), S138 (= S141), Y151 (= Y154), K155 (= K158), P181 (= P184), G182 (= G185)

P40288 Glucose 1-dehydrogenase; EC 1.1.1.47 from Priestia megaterium (Bacillus megaterium) (see 2 papers)
38% identity, 96% coverage: 2:244/254 of query aligns to 7:250/261 of P40288

query
sites
P40288

G

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11:35 (vs. 6:30, 40% identical) binding NADP(+)
E96 (≠ Q92) mutation E->A,G,K: Heat stable.
D108 (≠ G104) mutation to N: Heat stable.
V112 (= V108) mutation to A: Heat stable.
E133 (= E129) mutation to K: Heat stable.
V183 (= V179) mutation to I: Heat stable.
P194 (≠ E190) mutation to Q: Heat stable.
E210 (≠ A204) mutation to K: Heat stable.
Y217 (≠ R211) mutation to H: Heat stable.

Sites not aligning to the query:

252 Q→L: Heat stable.
253 Y→C: Heat stable.
258 A→G: Heat stable.

7v0hG Crystal structure of putative glucose 1-dehydrogenase from burkholderia cenocepacia in complex with NADP and a potential reaction product
40% identity, 96% coverage: 2:244/254 of query aligns to 11:252/253 of 7v0hG

query
sites
7v0hG

G

G

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G

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S

L

A

A

V

L

E

E

L

L

G

G

P

P

H

R

G

K

I

I

R

R

V

V

N

N

C

A

V

I

A

N

P
|
P

G
|
G

A
x
M

I
|
I

L

V

N
x
T

E

E

R
x
G

T
|
T

A

H

Q

S

E

A

-

G

-
x
I

-

I

Q

G

P

S

D

D

Y

L

A

E

Q

A

S

Q

W

V

A

L

P

G

I

Q

T

T

P

P

L

L

R

G

R

R

V

L

G

G

V

E

P

P

E

N

D

D

I

I

S

A

G

S

P

V

V

A

L

V

F

F

L

L

V

A

S

S

D

D

E

D

A

A

R

R

F

W

V

M

T

T

G

G

Q

E

T

H

L

L

W

V

V

V

D

S

G

G

L

L

binding nadp nicotinamide-adenine-dinucleotide phosphate: G18 (= G9), S20 (= S11), K21 (≠ T12), G22 (= G13), I23 (= I14), A43 (≠ R34), S44 (≠ H35), S45 (≠ D36), G68 (≠ C59), D69 (= D60), V70 (= V61), N96 (= N88), S97 (≠ A89), G98 (= G90), Y100 (≠ Q92), I144 (= I139), S146 (= S141), Y159 (= Y154), K163 (= K158), P189 (= P184), G190 (= G185), M191 (≠ A186), I192 (= I187), T194 (≠ N189), G196 (≠ R191), T197 (= T192)
binding (2R)-2-(hydroxymethyl)pentanedioic acid: S146 (= S141), Y159 (= Y154), M191 (≠ A186), I202 (vs. gap)

1g6kA Crystal structure of glucose dehydrogenase mutant e96a complexed with NAD+
38% identity, 96% coverage: 2:244/254 of query aligns to 7:250/261 of 1g6kA

query
sites
1g6kA

G

G

E

K

F

V

A

V

V

V

V

I

T

T

G

G

G

S

S

S

T
|
T

G
|
G

I
x
L

G

G

R

K

H

S

L

M

V

A

S

I

A

R

F

F

A

A

D

T

A

E

G

K

Y

A

A

K

V

V

A

V

F

V

S

N

Y

Y

R
|
R

H

S

D

K

D

E

D

D

A

E

A

A

Q

N

S

S

L

V

V

L

E

E

A

E

I

I

E

K

A

V

G

G

Q

E

A

A

L

I

G

A

L

V

G

K

C

G

D
|
D

V
|
V

G

T

R

V

R

E

A

S

E

D

V

V

E

I

A

N

F

L

F

V

D

Q

E

S

A

A

C

I

D

K

W

E

F

F

G

G

D

K

A

L

P

-

D

D

V

V

L

M

V

I

N

N

N
|
N

A
|
A

G
|
G

I

L

Q

A

T

N

W

P

A

V

P

S

L

S

L

H

E

E

L

M

S

S

E

L

E

S

G

D

W

W

D

N

D

K

V

V

I

I

R

D

T

T

N

N

L

L

K

T

G

G

C

A

F

F

L

L

N

G

T

S

Q

R

A

E

A

A

A

I

K

K

R

Y

M

F

V

V

E

E

A

N

D

D

K

I

G

K

G

G

A

T

I

V

V

I

N

N

I
x
M

G

S

S
|
S

G

V

C

H

N

E

K

K

L

I

A

P

F

W

P

P

K

L

L

F

V

V

S

H

Y
|
Y

T

A

A

A

S

S

K
|
K

G

G

I

M

E

K

Q

L

L

M

T

T

K

E

A

T

S

L

A

A

V

L

E

E

L

Y

G

A

P

P

H

K

G

G

I

I

R

R

V

V

N

N

C

N

V

I

A

G

P
|
P

G
|
G

A

A

I
|
I

L

N

N
x
T

E

P

R

I

T

N

A

A

Q

E

E

K

-

F

-

A

Q

D

P

P

D

E

Y

Q

A

R

Q

A

S

D

W

V

A

E

P

S

I

M

T

I

P

P

L

M

R

G

R

Y

V

I

G

G

V

E

P

P

E

E

D

E

I

I

S

A

G

A

P

V

V

A

L

A

F

W

L

L

V

A

S

S

D

S

E

E

A

A

R

S

F

Y

V

V

T

T

G

G

Q

I

T

T

L

L

W

F

V

A

D

D

G

G

L

M

active site: G18 (= G13), S145 (= S141), Y158 (= Y154), K162 (= K158)
binding nicotinamide-adenine-dinucleotide: T17 (= T12), G18 (= G13), L19 (≠ I14), R39 (= R34), D65 (= D60), V66 (= V61), N92 (= N88), A93 (= A89), G94 (= G90), M143 (≠ I139), S145 (= S141), Y158 (= Y154), P188 (= P184), G189 (= G185), I191 (= I187), T193 (≠ N189)

3ay6B Crystal structure of bacillus megaterium glucose dehydrogenase 4 a258f mutant in complex with nadh and d-glucose (see paper)
37% identity, 98% coverage: 3:252/254 of query aligns to 14:264/267 of 3ay6B

query
sites
3ay6B

E

K

F

V

A

V

V

V

V

I

T

T

G
|
G

G

G

S

S

T
|
T

G
|
G

I
x
L

G

G

R

R

H

A

L

M

V

A

S

V

A

R

F

F

A

G

D

Q

A

E

G

E

Y

A

A

K

V

V

A

V

F

I

S

N

Y

Y

R
x
Y

H

N

D

N

D

E

A

E

A

A

Q

L

S

D

L

A

V

K

E

K

A

E

I

V

E

E

A

A

G

G

Q

Q

A

A

L

I

G

I

L

V

G

Q

C

G

D
|
D

V
|
V

G

T

R

K

R

E

A

E

E

D

V

V

E

V

A

N

F

L

F

V

D

Q

E

T

A

A

C

I

D

K

W

E

F

F

G

G

D

T

A

L

P

-

D

D

V

V

L

M

V

I

N

N

N
|
N

A
|
A

G
|
G

I
x
V

Q
x
E

T

N

W

P

A

V

P

P

L

S

L

H

E

E

L

L

S

S

E

L

E

D

G

N

W

W

D

N

D

K

V

V

I

I

R

D

T

T

N

N

L

L

K

T

G

G

C

A

F

F

L

L

N

G

T

S

Q

R

A

E

A

A

A

I

K

K

R

Y

M

F

V

V

E

E

A

N

D

D

K

I

G

K

G

G

A

N

I

V

V

I

N

N

I
x
M

G

S

S
|
S

G

V

C
x
H

N

E

K

M

L

I

A

P

F
x
W

P

P

K

L

L

F

V

V

S

H

Y
|
Y

T

A

A

A

S

S

K
|
K

G

G

I

M

E

K

Q

L

L

M

T

T

K

E

A

T

S

L

A

A

V

L

E

E

L

Y

G

A

P

P

H

K

G

G

I

I

R

R

V

V

N

N

C

N

V

I

A

G

P
|
P

G
|
G

A

A

I
x
M

L

N

N
x
T

E
x
P

R
x
I

T
x
N

A

A

Q

E

E
x
K

Q

F

P

A

D

D

Y

P

A

V

Q

Q

-

R

-

A

S

D

W

V

A

E

P

S

I

M

T

I

P

P

L

M

R

G

R

Y

V

I

G

G

V

K

P

P

E

E

D

E

I

V

S

A

G

A

P

V

V

A

L

A

F

F

L

L

V

A

S

S

D

S

E

Q

A

A

R

S

F

Y

V

V

T

T

G

G

Q

I

T

T

L

L

W

F

V

A

D

D

G

G

L

M

F

T

S

K

Q

Y

A

P

N

S

W

F

P

Q

Y

F

active site: G24 (= G13), S151 (= S141), Y164 (= Y154), K168 (= K158)
binding beta-D-glucopyranose: E102 (≠ Q92), S151 (= S141), H153 (≠ C143), W158 (≠ F148), Y164 (= Y154), N202 (≠ T192), K205 (≠ E195)
binding 1,4-dihydronicotinamide adenine dinucleotide: G20 (= G9), T23 (= T12), G24 (= G13), L25 (≠ I14), Y45 (≠ R34), D71 (= D60), V72 (= V61), N98 (= N88), A99 (= A89), G100 (= G90), V101 (≠ I91), M149 (≠ I139), S151 (= S141), Y164 (= Y154), K168 (= K158), P194 (= P184), G195 (= G185), M197 (≠ I187), T199 (≠ N189), P200 (≠ E190), I201 (≠ R191), N202 (≠ T192)

7do7A Crystal structure of azotobacter vinelandii l-rhamnose 1- dehydrogenase(NAD and l-rhamnose bound-form) (see paper)
39% identity, 94% coverage: 6:245/254 of query aligns to 9:252/256 of 7do7A

query
sites
7do7A

V

I

V

V

T

T

G
|
G

G

A

S

S

T
x
R

G
|
G

I
|
I

G

G

R

R

H

A

L

A

V

A

S

R

A

E

F

C

A

A

D

R

A

Q

G

G

Y

A

A

R

V

V

A

V

F

I

S

G

Y

H

R
x
S

H

G

D

S

D

D

D

E

A

G

-

R

-

A

-

G

A

A

Q

L

S

S

L

L

V

A

E

E

A

E

I

I

E

A

A

F

G

G

Q

T

A

A

L

I

G

A

L

V

G

G

C

A

D
|
D

V
x
A

G

A

R

D

R

L

A

D

E

S

V

G

E

E

A

K

F

L

F

V

D

A

E

A

A

A

C

V

D

E

W

A

F

F

G

G

D

S

A

V

P

-

D

D

V

V

L

L

V

V

N

N

N
|
N

A
|
A

G
|
G

I
|
I

Q

C

T

P

W
x
F

A

H

P

S

L

F

L

L

E

D

L

M

S

P

E

R

E

E

G

L

W

Y

D

L

D

K

V

T

I

V

R

G

T

T

N

N

L

L

K

N

G

G

C

A

F

Y

L

F

N

T

T

V

Q

Q

A

A

K

R

R

R

M

M

V

K

E

E

A

Q

D

G

K

R

G

G

A

A

I

I

V

I

N

A

I
x
V

G
x
S

S
|
S

G

I

C
x
S

N

A

K

L

L

V

A

G

F

G

P

A

K

M

L
x
Q

V

T

S

H

Y
|
Y

T

T

A

P

S

T

K
|
K

G

A

G

G

I

L

E

L

Q

S

L

L

T

M

K

Q

A

S

S

C

A

A

V

I

E

A

L

L

G

G

P

P

H

Y

G

G

I

I

R

R

V

C

N

N

C

A

V

V

A

L

P
|
P

G
|
G

A

T

I
|
I

L

A

N
x
T

E

D

R
x
I

T
x
N

A

K

Q

E

E

D

Q

L

P

S

D

D

Y

L

-

E

-

K

A

R

Q

E

S

R

W

M

A

T

P

S

I

R

T

V

P

P

L

L

R

G

R

R

V

L

G

G

V

E

P

P

E

D

D

D

I

L

S

A

G

G

P

P

V

I

L

V

F

F

L

L

V

A

S

S

D
|
D

E
x
M

A

A

R
|
R

F

Y

V

V

T

T

G

G

Q

A

T

S

L

L

W

L

V

V

D

D

G

G

L

L

F

F

active site: G16 (= G13), S146 (= S141), Y159 (= Y154)
binding nicotinamide-adenine-dinucleotide: G12 (= G9), R15 (≠ T12), G16 (= G13), I17 (= I14), S37 (≠ R34), D66 (= D60), A67 (≠ V61), N93 (= N88), A94 (= A89), G95 (= G90), I96 (= I91), V144 (≠ I139), S145 (≠ G140), S146 (= S141), Y159 (= Y154), K163 (= K158), P189 (= P184), G190 (= G185), I192 (= I187), T194 (≠ N189), I196 (≠ R191)
binding beta-L-rhamnopyranose: F99 (≠ W94), S146 (= S141), S148 (≠ C143), Q156 (≠ L151), Y159 (= Y154), N197 (≠ T192), D235 (= D228), M236 (≠ E229), R238 (= R231)

7b81A Crystal structure of azotobacter vinelandii l-rhamnose 1-dehydrogenase (NAD bound-form) (see paper)
39% identity, 94% coverage: 6:245/254 of query aligns to 9:252/256 of 7b81A

query
sites
7b81A

V

I

V

V

T

T

G
|
G

G

A

S
|
S

T
x
R

G
|
G

I
|
I

G

G

R

R

H

A

L

A

V

A

S

R

A

E

F

C

A

A

D

R

A

Q

G

G

Y

A

A

R

V

V

A

V

F

I

S

G

Y

H

R

S

H

G

D

S

D

D

D

E

A

G

-

R

-

A

-

G

A

A

Q

L

S

S

L

L

V

A

E

E

A

E

I

I

E

A

A

F

G

G

Q

T

A

A

L

I

G

A

L

V

G

G

C

A

D
|
D

V
x
A

G

A

R

D

R

L

A

D

E

S

V

G

E

E

A

K

F

L

F

V

D

A

E

A

A

A

C

V

D

E

W

A

F

F

G

G

D

S

A

V

P

-

D

D

V

V

L

L

V

V

N

N

N
|
N

A
|
A

G
|
G

I
|
I

Q

C

T

P

W

F

A

H

P

S

L

F

L

L

E

D

L

M

S

P

E

R

E

E

G

L

W

Y

D

L

D

K

V

T

I

V

R

G

T
|
T

N

N

L

L

K

N

G

G

C

A

F

Y

L

F

N

T

T

V

Q

Q

A

A

K

R

R

R

M

M

V

K

E

E

A

Q

D

G

K

R

G

G

A

A

I

I

V

I

N

A

I
x
V

G

S

S
|
S

G

I

C

S

N

A

K

L

L

V

A

G

F

G

P

A

K

M

L

Q

V

T

S

H

Y
|
Y

T

T

A

P

S

T

K
|
K

G

A

G

G

I

L

E

L

Q

S

L

L

T

M

K

Q

A

S

S

C

A

A

V

I

E

A

L

L

G

G

P

P

H

Y

G

G

I

I

R

R

V

C

N

N

C

A

V

V

A

L

P
|
P

G
|
G

A

T

I
|
I

L

A

N
x
T

E

D

R
x
I

T

N

A

K

Q

E

E

D

Q

L

P

S

D

D

Y

L

-

E

-

K

A

R

Q

E

S

R

W

M

A

T

P

S

I

R

T

V

P

P

L

L

R

G

R

R

V

L

G

G

V

E

P

P

E

D

D

D

I

L

S

A

G

G

P

P

V

I

L

V

F

F

L

L

V

A

S

S

D

D

E

M

A

A

R

R

F

Y

V

V

T

T

G

G

Q

A

T

S

L

L

W

L

V

V

D

D

G

G

L

L

F

F

active site: G16 (= G13), S146 (= S141), Y159 (= Y154)
binding nicotinamide-adenine-dinucleotide: G12 (= G9), S14 (= S11), R15 (≠ T12), I17 (= I14), D66 (= D60), A67 (≠ V61), N93 (= N88), A94 (= A89), G95 (= G90), I96 (= I91), T116 (= T111), V144 (≠ I139), S146 (= S141), Y159 (= Y154), K163 (= K158), P189 (= P184), G190 (= G185), I192 (= I187), T194 (≠ N189), I196 (≠ R191)

7nm8AAA Antimycin pathway standalone ketoreductase, AntM (see paper)
40% identity, 94% coverage: 2:241/254 of query aligns to 6:243/251 of 7nm8AAA

query
sites
7nm8AAA

G

G

E

R

F

T

A

A

V

L

V

V

T

T

G
|
G

G

G

S
|
S

T
x
R

G
|
G

I
|
I

G

G

R

R

H

A

L

I

V

S

S

R

A

R

F

L

A

A

D

A

A

E

G

G

Y

A

A

L

V

V

A

A

F

V

S
x
H

Y
|
Y

R
x
G

H
|
H

D

D

D

H

D

A

A

A

Q

E

S

R

L

T

V

V

E

K

A

E

I

I

E

E

E

T

A

D

G

G

Q

R

A

A

L

F

G

P

L

V

G

H

C

A

D

E

V
x
L

G

G

R

V

R

E

-

G

-

D

-

A

A

A

E

T

V

L

E

W

A

A

F

A

F

F

D

D

E

E

A

A

C

L

D

S

W

G

F

T

G

G

D

S

A

G

P

P

-

G

-

L

D

D

V

I

L

L

V

V

N

N

N
|
N

A

A

G
|
G

I

I

Q

T

T

L

W

P

A

R

P

P

L

I

L

A

E

A

L

V

S

T

E

E

E

A

G

E

W

Y

D

D

D

R

V

V

I

F

R

A

T

V

N

N

L

T

K

R

G

A

C

P

F

F

L

F

N

I

T

V

Q

Q

A

R

A

S

A

L

K

E

R

R

M

L

V

R

E

D

A

-

D

-

K

-

G

G

A

R

I

I

V

I

N

S

I

I

G

S

S
|
S

G

A

C

A

N

T

K

Q

L

T

A

A

F

Y

P

P

K

A

L

I

V

V

S

A

Y
|
Y

T

S

A

M

S

S

K
|
K

G

G

G

A

I

L

E

D

Q

V

L

L

T

T

K

P

A

A

S

L

A

A

V

K

E

Q

L

L

G

G

P

P

H

R

G

G

I

I

R

T

V

V

N

N

C

T

V

V

A

A

P

P

G
|
G

A

-

I

-

L

F

N
x
T

E

E

R
x
T

T

E

A

I

Q

L

E

S

Q

N

P

P

D

E

Y

I

A

R

Q

K

S

A

W

L

A

S

P

S

I

A

T

S

P

V

L

F

R

G

R

R

V

L

G

G

V

A

P

P

E

A

D

D

I

I

S

A

G

D

P

V

V

V

L

S

F

F

L

V

V

A

S

S

D

D

E

E

A

A

R

R

F

W

V

V

T

T

G

G

Q

Q

T

-

L

-

W

W

V

I

D

D

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G13 (= G9), S15 (= S11), R16 (≠ T12), G17 (= G13), I18 (= I14), H36 (≠ S32), Y37 (= Y33), G38 (≠ R34), H39 (= H35), L65 (≠ V61), N97 (= N88), G99 (= G90), S147 (= S141), Y160 (= Y154), K164 (= K158), G191 (= G185), T193 (≠ N189), T195 (≠ R191)

7do6A Crystal structure of azotobacter vinelandii l-rhamnose 1- dehydrogenase(NADP bound-form) (see paper)
40% identity, 94% coverage: 6:245/254 of query aligns to 9:243/247 of 7do6A

query
sites
7do6A

V

I

V

V

T

T

G
|
G

G

A

S
|
S

T
x
R

G
|
G

I
|
I

G

G

R

R

H

A

L

A

V

A

S

R

A

E

F

C

A

A

D

R

A

Q

G

G

Y

A

A

R

V

V

A

V

F

I

S

G

Y
x
H

R
x
S

H

G

D

S

D

D

D

E

A
x
G

-

R

-

A

-

G

A

A

Q

L

S

S

L

L

V

A

E

E

A

E

I

I

E

A

A

F

G

G

Q

T

A

A

L

I

G

A

L

V

G

G

C

A

D
|
D

V
x
A

G

A

R

D

R

L

A

D

E

S

V

G

E

E

A

K

F

L

F

V

D

A

E

A

A

A

C

V

D

E

W

A

F

F

G

G

D

S

A

V

P

-

D

D

V

V

L

L

V

V

N

N

N
|
N

A
|
A

G
|
G

I
|
I

Q

C

T

P

W

F

A

H

P

S

L

F

L

L

E

D

L

M

S

P

E

R

E

E

G

L

W

Y

D

L

D

K

V

T

I

V

R

G

T
|
T

N

N

L

L

K

N

G

G

C

A

F

Y

L

F

N

T

T

V

Q

Q

A

A

K

R

R

R

M

M

V

K

E

E

A

Q

D

G

K

R

G

G

A

A

I

I

V

I

N

A

I

V

G

S

S
|
S

G

I

C

S

N

A

K

L

L

V

A

G

F

G

P

A

K

M

L

Q

V

T

S

H

Y
|
Y

T

T

A

P

S

T

K
|
K

G

A

G

G

I

L

E

L

Q

S

L

L

T

M

K

Q

A

S

S

C

A

A

V

I

E

A

L

L

G

G

P

P

H

Y

G

G

I

I

R

R

V

C

N

N

C

A

V

V

A

L

P

P

G

G

A

T

I
|
I

L

A

N

D

E

-

R

-

T

-

A

-

Q

L

E

E

Q

K

P

R

D

E

Y

R

A

M

Q

T

S

S

W

R

A

-

P

-

I

-

T

V

P

P

L

L

R

G

R

R

V

L

G

G

V

E

P

P

E

D

D

D

I

L

S

A

G

G

P

P

V

I

L

V

F

F

L

L

V

A

S

S

D

D

E

M

A

A

R

R

F

Y

V

V

T

T

G

G

Q

A

T

S

L

L

W

L

V

V

D

D

G

G

L

L

F

F

active site: G16 (= G13), S146 (= S141), Y159 (= Y154)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G9), S14 (= S11), R15 (≠ T12), G16 (= G13), I17 (= I14), H36 (≠ Y33), S37 (≠ R34), G42 (≠ A39), D66 (= D60), A67 (≠ V61), N93 (= N88), A94 (= A89), G95 (= G90), I96 (= I91), T116 (= T111), S146 (= S141), Y159 (= Y154), K163 (= K158), I192 (= I187)

8w0oA Gdh-105 crystal structure
35% identity, 96% coverage: 2:244/254 of query aligns to 7:250/259 of 8w0oA

query
sites
8w0oA

G

G

E

K

F

V

A

V

V

A

V

I

T

T

G
|
G

G

A

S

A

T
x
S

G
|
G

I
x
L

G

G

R

K

H

A

L

M

V

A

S

I

A

R

F

F

A

G

D

K

A

E

G

Q

Y

A

A

K

V

V

A

V

F

I

S

N

Y

Y

R
x
Y

H

S

D

N

D

K

D
x
Q

A

D

A

P

Q

N

S

E

L

V

V

K

E

E

A

E

I

V

E

I

E

K

A

A

G

G

Q

E

A

A

L

V

G

V

L

V

G

Q

C

G

D
|
D

V
|
V

G

T

R

K

R

E

A

E

E

D

V

V

E

K

A

N

F

I

F

V

D

Q

E

T

A

A

C

I

D

K

W

E

F

F

G

G

D

T

A

L

P

-

D

D

V

I

L

M

V

I

N

N

N
|
N

A
|
A

G
|
G

I

L

Q

E

T

N

W

P

A

V

P

P

L

S

L

H

E

E

L

M

S

P

E

L

E

K

G

D

W

W

D

D

D

K

V

V

I

I

R

G

T

T

N

N

L

L

K

T

G

G

C

A

F

F

L

L

N

G

T

S

Q

R

A

E

A

A

A

I

K

K

R

Y

M

F

V

V

E

E

A

N

D

D

K

I

G

K

G

G

A

N

I

V

V

I

N

N

I
x
M

G

S

S

S

G

V

C

H

N

E

K

V

L

I

A

P

F

W

P

P

K

L

L

F

V

V

S

H

Y
|
Y

T

A

A

A

S

S

K
|
K

G

G

I

M

E

K

Q

L

L

M

T

T

K

K

A

T

S

L

A

A

V

L

E

E

L

Y

G

A

P

P

H

K

G

G

I

I

R

R

V

V

N

N

C

N

V

I

A

G

P
|
P

G
|
G

A

A

I
|
I

L

N

N
x
T

E

T

R

I

T

N

A

A

Q

E

E

K

Q

F

P

A

D

D

Y

P

A

K

Q

Q

S

K

-

A

-

D

W

V

A

E

P

S

I

M

T

I

P

P

L

M

R

G

R

Y

V

I

G

G

V

E

P

P

E

E

D

E

I

I

S

A

G

A

P

V

V

A

L

A

F

W

L

L

V

A

S

S

D

K

E

E

A

A

R

S

F

Y

V

V

T

T

G

G

Q

I

T

T

L

L

W

F

V

A

D

D

G

G

L

M

binding nicotinamide-adenine-dinucleotide: G14 (= G9), S17 (≠ T12), G18 (= G13), L19 (≠ I14), Y39 (≠ R34), Q43 (≠ D38), D65 (= D60), V66 (= V61), N92 (= N88), A93 (= A89), G94 (= G90), M143 (≠ I139), Y158 (= Y154), K162 (= K158), P188 (= P184), G189 (= G185), I191 (= I187), T193 (≠ N189)

1edoA The x-ray structure of beta-keto acyl carrier protein reductase from brassica napus complexed with NADP+ (see paper)
40% identity, 94% coverage: 6:244/254 of query aligns to 5:242/244 of 1edoA

query
sites
1edoA

V

V

T

T

G
|
G

G

A

S
|
S

T
x
R

G
|
G

I
|
I

G

G

R

K

H

A

L

I

V

A

S

L

A

S

F

L

A

G

D

K

A

A

G

G

Y

C

A

K

V

V

A

L

F

V

S
x
N

Y
|
Y

R
x
A

H
x
R

D
x
S

D

A

D

K

A

A

Q

E

S

E

L

V

V

S

E

K

A

Q

I

I

E

E

E

A

A

Y

G

G

Q

Q

A

A

L

I

G

T

L

F

G

G

C

G

D
|
D

V
|
V

G

S

R

K

R

E

A

A

E

D

V

V

E

E

A

A

F

M

D

K

E

T

A

A

C

I

D

D

W

A

F

W

G

G

D

T

A

I

P

-

D

D

V

V

L

V

V

V

N

N

N
|
N

A
|
A

G

G

I

I

Q

T

T

R

W

D

A

T

P

L

L

L

L

I

E

R

L

M

S

K

E

K

E

S

G

Q

W

W

D

D

D

E

V

V

I

I

R

D

T

L

N

N

L

L

K

T

G

G

C

V

F

F

L

L

N

C

T

T

Q

Q

A

A

A

T

K

K

R

I

M

M

V

M

E

K

A

K

D

R

K

K

G

-

G

G

A

R

I

I

V

I

N

N

I

I

G

A

S
|
S

G

V

C

V

N

G

K

L

L

I

A

G

F

N

P

I

K

G

L

Q

V

A

S

N

Y
|
Y

T

A

A

A

S

A

K
|
K

G

A

G

G

I

V

E

I

Q

G

L

F

T

S

K

K

A

T

S

A

A

A

V

R

E

E

L

G

G

A

P

S

H

R

G

N

I

I

R

N

V

V

N

N

C

V

V

V

A

C

P
|
P

G
|
G

A

F

I
|
I

L

A

N
x
S

E

D

R
x
M

T

T

A

A

Q

K

E

L

Q

G

P

E

D

D

Y

M

A

E

Q

K

S

K

W

I

A

L

P

G

I

T

T

I

P

P

L

L

R

G

R

R

V

T

G

G

V

Q

P

P

E

E

D

N

I

V

S

A

G

G

P

L

V

V

L

E

F

F

L

L

-

A

V

L

S

S

D

P

E

A

A

A

R

S

F

Y

V

I

T

T

G

G

Q

Q

T

A

L

F

W

T

V

I

D

D

G

G

L

I

active site: G12 (= G13), S138 (= S141), Y151 (= Y154), K155 (= K158)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G9), S10 (= S11), R11 (≠ T12), I13 (= I14), N31 (≠ S32), Y32 (= Y33), A33 (≠ R34), R34 (≠ H35), S35 (≠ D36), D59 (= D60), V60 (= V61), N86 (= N88), A87 (= A89), S138 (= S141), Y151 (= Y154), K155 (= K158), P181 (= P184), G182 (= G185), I184 (= I187), S186 (≠ N189), M188 (≠ R191)

1vl8B Crystal structure of gluconate 5-dehydrogenase (tm0441) from thermotoga maritima at 2.07 a resolution
37% identity, 95% coverage: 2:243/254 of query aligns to 6:248/252 of 1vl8B

query
sites
1vl8B

G

G

E

R

F

V

A

A

V

L

V

V

T

T

G
|
G

G

G

S

S

T
x
R

G
|
G

I
x
L

G

G

R

F

H

G

L

I

V

A

S

Q

A

G

F

L

A

A

D

E

A

A

G

G

Y

C

A

S

V

V

A

V

F

V

S

A

Y
x
S

R
|
R

H

N

D

L

D

E

A

A

A

S

Q

E

S

A

L

A

V

Q

E

K

A

L

I

T

E

E

E

K

A

Y

G

G

G

V

Q

E

A

T

L

M

G

A

L

F

G

R

C
|
C

D
|
D

V
|
V

G

S

R

N

R

Y

A

E

E

E

V

V

E

K

A

K

F

L

D

E

E

A

A

V

C

K

D

E

W

K

F

F

G

G

D

K

A

L

P

-

D

D

V

T

L

V

V

V

N

N

N
x
A

A
|
A

G
|
G

I
|
I

Q

N

T

R

W

R

A

H

P

P

L

A

L

E

E

E

L

F

S

P

E

L

E

D

G

E

W

F

D

R

D

Q

V

V

I

I

R

E

T
x
V

N

N

L

L

K

F

G

G

C

T

F

Y

L

Y

N

V

T

C

Q

R

A

E

A

A

A

F

K

S

R

L

M

L

V

R

E

E

A

S

D

D

K

N

G

P

G

-

A

S

I

I

V

I

N

N

I
|
I

G

G

S
|
S

-

L

G

T

C

V

N

E

K

E

L

V

A

T

F

M

P

P

K

N

L
x
I

V

S

S

A

Y
|
Y

T

A

A

A

S

S

K
|
K

G

G

I

V

E

A

Q

S

L

L

T

T

K

K

A

A

S

L

A

A

V

K

E

E

L

W

G

G

P

R

H

Y

G

G

I

I

R

R

V

V

N

N

C

V

V

I

A

A

P
|
P

G
|
G

A

W

I
x
Y

L

R

N
x
T

E

K

R
x
M

T
|
T

A

E

Q

A

-

V

-

F

E

S

Q

D

P

P

D

E

Y

K

A

L

Q

D

S

Y

W

M

A

L

P

K

I

R

T

I

P

P

L

L

R

G

R

R

V

T

G

G

V

V

P

P

E

E

D

D

I

L

S

K

G

G

P

V

V

A

L

V

F

F

L

L

V

A

S

S

D

E

E

E

A

A

R

K

F

Y

V

V

T

T

G

G

Q

Q

T

I

L

I

W

F

V

V

D

D

G

G

active site: G17 (= G13), S143 (= S141), I154 (≠ L151), Y157 (= Y154), K161 (= K158)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G9), R16 (≠ T12), G17 (= G13), L18 (≠ I14), S37 (≠ Y33), R38 (= R34), C63 (= C59), D64 (= D60), V65 (= V61), A91 (≠ N88), A92 (= A89), G93 (= G90), I94 (= I91), V114 (≠ T111), I141 (= I139), S143 (= S141), Y157 (= Y154), K161 (= K158), P187 (= P184), G188 (= G185), Y190 (≠ I187), T192 (≠ N189), M194 (≠ R191), T195 (= T192)

5itvA Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
38% identity, 95% coverage: 6:247/254 of query aligns to 11:255/255 of 5itvA

query
sites
5itvA

V

L

V

I

T

T

G
|
G

G

G

S

A

T
x
S

G
|
G

I
|
I

G

G

R

Y

H

A

L

A

V

V

S

Q

A

A

F

F

A

L

D

G

A

Q

G

Q

Y

A

A

N

V

V

A

V

F

V

S

A

Y

-

R

-

H

-

D
|
D

D
x
I

D

D

A

E

A

A

Q

Q

S

G

L

E

V

A

E

M

A

V

I

R

E

K

E

E

A

N

G

N

G

D

Q

R

A

L

L

H

G

F

L

V

G

Q

C
x
T

D

D

V
x
I

G

T

R

D

R

E

A

A

E

A

V

C

E

Q

A

H

F

A

F

V

D

E

E

S

A

A

C

V

D

H

W

T

F

F

G

G

D

-

A

G

P

L

D

D

V

V

L

L

V

I

N

N

N
|
N

A

A

G
|
G

I

I

Q

E

T

I

W

V

A

A

P

P

L

I

L

H

E

E

L

M

S

E

E

L

E

S

G

D

W

W

D

N

D

K

V

V

I

L

R

Q

T

V

N

N

L

L

K

T

G

G

C

M

F

F

L

L

N

M

T

S

Q

K

A

H

A

A

A

L

K

K

R

H

M

M

V

L

E

A

A

A

D

G

K

K

G

G

G

N

A

-

I

I

V

I

N

N

I
x
T

G

C

S
|
S

G

V

C

G

N

G

K

L

L

V

A

A

F

W

P

P

K

D

L

I

V

P

S

A

Y
|
Y

T

N

A

A

S

S

K
|
K

G

G

I

V

E

L

Q

Q

L

L

T

T

K

K

A

S

S

M

A

A

V

V

E

D

L

Y

G

A

P

K

H

H

G

Q

I

I

R

R

V

V

N

N

C

C

V

V

A

C

P
|
P

G
|
G

A
x
I

I
|
I

-

D

-

T

-

P

L

L

N

N

E

E

R

K

T

S

A

F

Q

L

E

E

Q

N

-

N

-

E

-

G

-

T

-

L

P

E

D

E

Y

I

A

K

Q

K

S

E

W

K

A

A

P

K

I

V

T

N

P

P

L

L

R

L

R

R

V

L

G

G

V

K

P

P

E

E

D

E

I

I

S

A

G

N

P

V

V

M

L

L

F

F

L

L

V

A

S

S

D

D

E

L

A

S

R

S

F

Y

V

M

T

T

G

G

Q

S

T

A

L

I

W

T

V

A

D

D

G

G

L

Y

F

T

S

A

Q

Q

active site: G18 (= G13), S141 (= S141), Y154 (= Y154), K158 (= K158)
binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G9), S17 (≠ T12), G18 (= G13), I19 (= I14), D38 (= D36), I39 (≠ D37), T61 (≠ C59), I63 (≠ V61), N89 (= N88), G91 (= G90), T139 (≠ I139), S141 (= S141), Y154 (= Y154), K158 (= K158), P184 (= P184), G185 (= G185), I186 (≠ A186), I187 (= I187)

Q9KJF1 (2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA dehydrogenase subunit BbsD; (S,R)-2-(alpha-hydroxybenzyl)succinyl-CoA dehydrogenase subunit BbsD; EC 1.1.1.429 from Thauera aromatica (see 2 papers)
39% identity, 95% coverage: 3:243/254 of query aligns to 6:242/248 of Q9KJF1

query
sites
Q9KJF1

E

R

F

V

A

A

V

L

V

I

T

T

G

G

G

S

S

A

T
x
S

G

G

I

M

G

G

R

K

H

Q

L

T

V

A

S

L

A

R

F

F

A

A

D

E

A

Q

G

G

Y

A

A

A

V

V

A

V

F

I

S

N

Y

-

R
x
D

H

I

D

D

D

A

D

E

A

K

A

V

Q

R

S

A

L

T

V

V

E

D

A

E

I

F

E

S

E

A

A

R

G

G

G

H

Q

R

A

V

L

L

G

G

L

A

G

V

C

A

D
|
D

V
x
I

G

G

R

N

R

K

A

A

E

A

V

V

E

D

A

G

F

M

F

V

D

K

E

Q

A

T

C

I

D

D

W

A

F

F

G

G

D

R

A

I

P

-

D

D

V

I

L

L

V

V

N

N

N
|
N

A

A

G

G

I

M

Q

E

T

R

W

A

A

G

P

A

L

L

L

R

E

K

L

L

S

S

E

E

E

A

G

D

W

W

D

D

D

V

V

T

I

I

R

N

T

V

N

N

L

L

K

K

G

G

C

T

F

F

L

L

N

C

T

T

Q

Q

A

A

A

V

A

H

K

G

R

H

M

M

V

V

E

E

A

-

D

N

K

K

G

H

G

G

A

R

I

I

V

V

N

N

I

I

G

A

S

S

G

R

C

A

N

-

K

W

L

L

A

G

F

G

P

A

K

G

L

Q

V

T

S

P

Y
|
Y

T

S

A

S

S

A

K
|
K

G

A

G

G

I

V

E

V

Q

G

L

M

T

T

K

R

A

A

S

L

A

A

V

I

E

E

L

L

G

G

P

R

H

A

G

G

I

I

R

T

V

V

N

N

C

C

V

V

A

A

P

P

G

G

A

-

I

L

L

I

N

H

E

T

R

P

T

M

A

W

Q

D

E

E

Q

L

P

P

D

E

Y

K

A

D

Q

Q

S

Q

W

F

-

L

A

L

P

S

I

R

T

Q

P

P

L

T

R

G

R

K

V

L

G

G

V

E

P

P

E

D

D

D

I

I

S

A

G

N

P

T

V

L

L

L

F

F

L

L

V

A

S

D

D

D

E

D

A

S

R

G

F

F

V

V

T

T

G

G

Q

Q

T

V

L

L

W

Y

V

V

D

C

G

G

S15 (≠ T12) binding NAD(+)
D36 (≠ R34) binding NAD(+)
D62 (= D60) binding NAD(+)
I63 (≠ V61) binding NAD(+)
N89 (= N88) binding NAD(+)
Y153 (= Y154) binding NAD(+)
K157 (= K158) binding NAD(+)

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

7pcsB Structure of the heterotetrameric sdr family member bbscd (see paper)
39% identity, 95% coverage: 3:243/254 of query aligns to 5:241/247 of 7pcsB

query
sites
7pcsB

E

R

F

V

A

A

V

L

V

I

T

T

G
|
G

G

S

S

A

T

S

G

G

I
x
M

G

G

R

K

H

Q

L

T

V

A

S

L

A

R

F

F

A

A

D

E

A

Q

G

G

Y

A

A

A

V

V

A

V

F

I

S

N

Y

-

R
x
D

H
x
I

D

D

D

A

D

E

A

K

A

V

Q

R

S

A

L

T

V

V

E

D

A

E

I

F

E

S

E

A

A

R

G

G

G

H

Q

R

A

V

L

L

G

G

L

A

G

V

C

A

D

D

V
x