SitesBLAST – Find functional sites

 

SitesBLAST

Comparing WP_008537264.1 NCBI__GCF_000245775.1:WP_008537264.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

P69783 PTS system glucose-specific EIIA component; EIIA-Glc; EIII-Glc; Glucose-specific phosphotransferase enzyme IIA component from Escherichia coli (strain K12) (see 6 papers)
37% identity, 100% coverage: 1:160/160 of query aligns to 1:168/169 of P69783

query
sites
P69783
M
|
M
G
 
G
L
 
L
F
|
F
S
 
D
K
 
K
L
 
L
-
 
K
-
 
S
F
 
L
Q
 
V
S
 
S
N
 
D
E
 
D
K
 
K
N
 
K
-
 
D
-
 
T
-
 
G
-
 
T
-
 
I
-
 
E
F
 
I
V
 
I
S
 
A
P
 
P
M
 
L
T
 
S
G
 
G
K
 
E
V
 
I
V
 
V
A
 
N
M
 
I
S
 
E
D
 
D
I
 
V
P
 
P
D
 
D
P
 
V
A
 
V
F
 
F
A
 
A
Q
 
E
K
 
K
M
 
I
M
 
V
G
 
G
D
 
D
G
 
G
C
 
I
G
 
A
I
 
I
E
 
K
L
 
P
T
 
T
D
 
G
G
 
N
T
 
K
V
 
M
V
 
V
A
 
A
P
 
P
F
 
V
D
 
D
A
 
G
K
 
T
V
 
I
T
 
G
A
 
K
A
 
I
F
 
F
P
 
E
T
 
T
G
 
N
H
|
H
A
 
A
F
 
F
G
 
S
L
 
I
E
 
E
A
 
S
K
 
-
E
 
D
D
 
S
G
 
G
T
 
V
E
 
E
V
 
L
L
 
F
I
 
V
H
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H
I
 
F
G
 
G
I
 
I
D
 
D
T
 
T
V
 
V
E
 
E
M
 
L
E
 
K
G
 
G
E
 
E
G
 
G
F
 
F
S
 
K
S
 
R
N
 
I
I
 
A
K
 
E
V
 
E
G
 
G
D
 
Q
E
 
R
V
 
V
K
 
K
Q
 
V
G
 
G
D
 
D
V
 
T
L
 
V
V
 
I
V
 
E
V
 
F
D
 
D
L
 
L
E
 
P
V
 
L
V
 
L
Q
 
E
E
 
E
A
 
K
G
 
A
K
 
K
S
 
S
L
 
T
V
 
L
S
 
T
P
 
P
I
 
V
A
 
V
F
 
I
T
 
S
G
 
N
N
 
M
N
 
D
K
 
E
V
 
I
-
 
K
E
 
E
V
 
L
F
 
I
K
 
K
I
 
L
E
 
S
Q
 
G
N
 
S
V
 
V
V
 
T
A
 
V
G
 
G
E
 
E
K
 
T
G
 
P
L
 
V
L
 
I
N
 
R
Y
 
I
K
 
K

1glcF Cation promoted association (cpa) of a regulatory and target protein is controlled by phosphorylation (see paper)
37% identity, 99% coverage: 2:160/160 of query aligns to 1:160/161 of 1glcF

query
sites
1glcF
G
 
G
L
 
L
F
 
F
S
 
D
K
 
K
L
 
L
F
 
K
Q
 
S
-
 
L
S
 
V
N
 
S
E
 
T
K
 
I
N
 
E
F
 
I
V
 
I
S
 
A
P
 
P
M
 
L
T
 
S
G
 
G
K
 
E
V
 
I
V
 
V
A
 
N
M
 
I
S
 
E
D
 
D
I
 
V
P
 
P
D
 
D
P
 
V
A
 
V
F
 
F
A
 
A
Q
 
E
K
 
K
M
 
I
M
 
V
G
 
G
D
 
D
G
 
G
C
 
I
G
 
A
I
 
I
E
 
K
L
 
P
T
 
T
D
 
G
G
 
N
T
 
K
V
 
M
V
 
V
A
 
A
P
 
P
F
 
V
D
 
D
A
 
G
K
 
T
V
 
I
T
 
G
A
 
K
A
 
I
F
 
F
P
 
E
T
 
T
G
 
N
H
|
H
A
 
A
F
 
F
G
 
S
L
 
I
E
 
E
A
 
S
K
 
-
E
 
D
D
 
S
G
 
G
T
 
V
E
 
E
V
 
L
L
 
F
I
 
V
H
|
H
I
 
F
G
 
G
I
 
I
D
 
D
T
 
T
V
 
V
E
 
E
M
 
L
E
 
K
G
 
G
E
 
E
G
 
G
F
 
F
S
 
K
S
 
R
N
 
I
I
 
A
K
 
E
V
 
E
G
 
G
D
 
Q
E
 
R
V
 
V
K
 
K
Q
 
V
G
 
G
D
 
D
V
 
T
L
 
V
V
 
I
V
 
E
V
 
F
D
 
D
L
 
L
E
 
P
V
 
L
V
 
L
Q
 
E
E
 
E
A
 
K
G
 
A
K
 
K
S
 
S
L
 
T
V
 
L
S
 
T
P
 
P
I
 
V
A
 
V
F
 
I
T
 
S
G
 
N
N
 
M
N
 
D
K
 
E
V
 
I
-
 
K
E
 
E
V
 
L
F
 
I
K
 
K
I
 
L
E
 
S
Q
 
G
N
 
S
V
 
V
V
 
T
A
 
V
G
 
G
E
 
E
K
 
T
G
 
P
L
 
V
L
 
I
N
 
R
Y
 
I
K
 
K

1o2fA Complex of enzyme iiaglc and iibglc phosphocarrier protein hpr from escherichia coli nmr, restrained regularized mean structure (see paper)
37% identity, 91% coverage: 15:160/160 of query aligns to 4:149/150 of 1o2fA

query
sites
1o2fA
F
 
I
V
 
I
S
 
A
P
 
P
M
 
L
T
 
S
G
 
G
K
 
E
V
 
I
V
 
V
A
 
N
M
 
I
S
 
E
D
 
D
I
 
V
P
 
P
D
 
D
P
 
V
A
 
V
F
 
F
A
 
A
Q
 
E
K
 
K
M
 
I
M
 
V
G
 
G
D
 
D
G
 
G
C
 
I
G
 
A
I
 
I
E
 
K
L
 
P
T
 
T
D
 
G
G
 
N
T
 
K
V
 
M
V
 
V
A
 
A
P
 
P
F
 
V
D
 
D
A
 
G
K
 
T
V
 
I
T
 
G
A
 
K
A
 
I
F
 
F
P
 
E
T
|
T
G
 
N
H
|
H
A
 
A
F
 
F
G
 
S
L
 
I
E
 
E
A
 
S
K
 
-
E
 
D
D
 
S
G
 
G
T
 
V
E
 
E
V
 
L
L
 
F
I
 
V
H
|
H
I
 
F
G
 
G
I
 
I
D
|
D
T
 
T
V
 
V
E
 
E
M
 
L
E
 
K
G
 
G
E
 
E
G
 
G
F
 
F
S
 
K
S
 
R
N
 
I
I
 
A
K
 
E
V
 
E
G
 
G
D
 
Q
E
 
R
V
 
V
K
 
K
Q
 
V
G
 
G
D
 
D
V
 
T
L
 
V
V
 
I
V
 
E
V
 
F
D
 
D
L
 
L
E
 
P
V
 
L
V
 
L
Q
 
E
E
 
E
A
 
K
G
 
A
K
 
K
S
 
S
L
 
T
V
 
L
S
 
T
P
 
P
I
 
V
A
 
V
F
 
I
T
 
S
G
 
N
N
 
M
N
 
D
K
 
E
V
 
I
-
 
K
E
 
E
V
 
L
F
 
I
K
 
K
I
 
L
E
 
S
Q
 
G
N
 
S
V
 
V
V
 
T
A
 
V
G
 
G
E
 
E
K
 
T
G
 
P
L
 
V
L
 
I
N
 
R
Y
 
I
K
 
K

P09323 PTS system N-acetylglucosamine-specific EIICBA component; EIICBA-Nag; EII-Nag; EC 2.7.1.193 from Escherichia coli (strain K12) (see paper)
38% identity, 91% coverage: 15:160/160 of query aligns to 501:647/648 of P09323

query
sites
P09323
F
 
L
V
 
V
S
 
S
P
 
P
M
 
I
T
 
T
G
 
G
K
 
D
V
 
V
V
 
V
A
 
A
M
 
L
S
 
D
D
 
Q
I
 
V
P
 
P
D
 
D
P
 
E
A
 
A
F
 
F
A
 
A
Q
 
S
K
 
K
M
 
A
M
 
V
G
 
G
D
 
D
G
 
G
C
 
V
G
 
A
I
 
V
E
 
K
L
 
P
T
 
T
D
 
D
G
 
K
T
 
I
V
 
V
V
 
V
A
 
S
P
 
P
F
 
A
D
 
A
A
 
G
K
 
T
V
 
I
T
 
V
A
 
K
A
 
I
F
 
F
P
 
N
T
 
T
G
 
N
H
 
H
A
 
A
F
 
F
G
 
C
L
 
L
E
 
E
A
 
-
K
 
T
E
 
E
D
 
K
G
 
G
T
 
A
E
 
E
V
 
I
L
 
V
I
 
V
H
 
H
I
 
M
G
 
G
I
 
I
D
 
D
T
 
T
V
 
V
E
 
A
M
 
L
E
 
E
G
 
G
E
 
K
G
 
G
F
 
F
S
 
K
S
 
R
N
 
L
I
 
V
K
 
E
V
 
E
G
 
G
D
 
A
E
 
Q
V
 
V
K
 
S
Q
 
A
G
 
G
D
 
Q
V
 
P
L
 
I
V
 
L
V
 
E
V
 
M
D
 
D
L
 
L
E
 
D
V
 
Y
V
 
L
Q
 
N
E
 
A
A
 
N
G
 
A
K
 
R
S
 
S
L
 
M
V
 
I
S
 
S
P
 
P
I
 
V
A
 
V
F
 
C
T
 
S
G
 
N
N
 
I
N
 
D
K
 
D
V
 
F
E
 
S
-
 
G
-
 
L
V
 
I
F
 
I
K
 
K
I
 
A
E
 
Q
Q
 
G
N
 
H
V
 
I
V
 
V
A
 
A
G
 
G
E
 
Q
K
 
T
G
 
P
L
 
L
L
 
Y
N
 
E
Y
 
I
K
 
K

Sites not aligning to the query:

Query Sequence

>WP_008537264.1 NCBI__GCF_000245775.1:WP_008537264.1
MGLFSKLFQSNEKNFVSPMTGKVVAMSDIPDPAFAQKMMGDGCGIELTDGTVVAPFDAKV
TAAFPTGHAFGLEAKEDGTEVLIHIGIDTVEMEGEGFSSNIKVGDEVKQGDVLVVVDLEV
VQEAGKSLVSPIAFTGNNKVEVFKIEQNVVAGEKGLLNYK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory