SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_008538557.1 NCBI__GCF_000245775.1:WP_008538557.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

A8B2U2 Fructose-bisphosphate aldolase; Glfba; glFBPA; Fructose-1,6-bisphosphate aldolase; EC 4.1.2.13 from Giardia intestinalis (strain ATCC 50803 / WB clone C6) (Giardia lamblia) (see 4 papers)
50% identity, 97% coverage: 1:312/321 of query aligns to 1:299/323 of A8B2U2

query
sites
A8B2U2

M

M

P

P

L

L

V

C

T

T

T

L

K

R

E

Q

M

M

F

L

K

G

K

E

A

A

Y

R

E

K

G

H

H

K

Y

Y

A

G

I

V

G

G

A

A

F

F

N

N

V

V

N

N

M

M

E

E

I

Q

V

I

Q

Q

G

G

I

I

T

M

E

K

A

A

A

V

K

V

E

Q

L

L

N

K

A

S

P

P

L

V

I

I

L

L

Q

Q

C

C

S
|
S

A

R

G

G

A

A

R

L

K

K

Y

Y

A

S

K

D

H

M

Q

I

Y

Y

L

L

V

K

H

K

L

L

V

C

Q

E

A

A

I

L

E

E

D

K

-

H

T

P

G

D

L

I

P

P

I

I

A

C

L

I

H

H

L

L

D
|
D

H
|
H

G

G

A

D

D

T

F

L

E

E

T

S

C

V

K

K

S

M

C

A

I

I

D

D

G

L

G

G

F

F

T

S

S

S

V

V

M

M

F

I

D

D

G

A

S

S

H

H

Y

H

S

P

F

F

K

D

E

E

N

N

I

V

E

R

K

I

T

T

K

K

E

E

V

V

E

A

Y

Y

A

A

H

H

A

A

H

R

G

G

V

V

V

S

V

V

E

E

A

A

E

E

L

L

G

G

Q

T

L

L

A

G

G

G

I

I

E

E

D

E

D

D

V

V

K

Q

V

-

A

-

H

N

E

T

A

V

H

Q

Y

L

T

T

D

E

P

P

N

Q

E

D

V

A

E

K

F

F

V

V

K

E

E

L

T

T

G

G

V

V

D

D

S

A

L

L

A

A

I

V

A

A

I

I

G

G

T

T

S

S

H
|
H

G
|
G

A

A

Y

Y

K
|
K

F

F

T

K

P

S

D

E

Q

S

C

D

T

I

R

R

N

-

E

-

Q

-

G

-

I

-

L

-

V

-

P

-

L

L

R

A

F

I

D

D

I

R

L

V

E

K

E

T

V

I

A

S

K

D

R

-

L

L

P

T

N

G

F

I

P

P

I

L

V

V

L

M

H
|
H

G
|
G

A

S

S
|
S

S

S

V

V

P

P

Q

K

D

D

F

V

V

K

K

D

I

M

I

I

N

N

E

K

N

Y

G

G

H

K

M

M

P

P

D

D

A

A

V

V

G

G

V

V

P

P

E

I

D

E

Q

S

L

I

R

V

K

H

A

A

A

I

S

G

M

E

A

G

V

V

C

C

K

K

I

I

N
|
N

I

V

D
|
D

S
|
S

D

D

L

S

R
|
R

L

M

A

A

M

M

T

T

A

G

G

A

I

I

R

R

K

K

H

V

F

F

N

V

D

E

H

H

P

P

D

E

H

K

F

F

D
|
D

P

P

R
|
R

Q

D

Y

Y

V

L

A

G

D

P

G

G

R

R

T

D

N

A

I

I

K

T

A

E

I

M

V

L

S

I

H

P

K

K

I

I

K

K

S50 (= S50) binding beta-D-fructose 1,6-bisphosphate
D83 (= D82) active site, Proton donor; mutation to A: Severe loss of catalytic activity.
H84 (= H83) binding Zn(2+)
H178 (= H180) binding beta-D-fructose 1,6-bisphosphate; binding Zn(2+)
G179 (= G181) binding beta-D-fructose 1,6-bisphosphate
K182 (= K184) binding beta-D-fructose 1,6-bisphosphate
H210 (= H223) binding Zn(2+)
G211 (= G224) binding beta-D-fructose 1,6-bisphosphate
S213 (= S226) binding beta-D-fructose 1,6-bisphosphate
N253 (= N266) binding beta-D-fructose 1,6-bisphosphate
D255 (= D268) binding beta-D-fructose 1,6-bisphosphate; mutation to A: 9.4-fold reduction in substrate affinity and 50-fold reduction in catalytic affinity. Has some activity towards tagatose-1,6-bisphosphate.
S256 (= S269) binding beta-D-fructose 1,6-bisphosphate
R259 (= R272) binding beta-D-fructose 1,6-bisphosphate; mutation to A: 1.8-fold reduction in substrate affinity and 2.8-fold reduction in catalytic efficiency. 6-fold reduction in substrate affinity and 24-fold reduction in catalytic efficiency; when associated with A-278.
D278 (= D291) mutation to A: 159-fold reduction in substrate affinity and 2770-fold reduction in catalytic efficiency. 6-fold reduction in substrate affinity and 24-fold reduction in catalytic efficiency; when associated with A-259.
R280 (= R293) binding beta-D-fructose 1,6-bisphosphate

3gayA Structure of giardia fructose-1,6-biphosphate aldolase in complex with tagatose-1,6-biphosphate (see paper)
50% identity, 97% coverage: 2:312/321 of query aligns to 1:295/319 of 3gayA

query
sites
3gayA

P

P

L

L

V

C

T

T

T

L

K

R

E

Q

M

M

F

L

K

G

K

E

A

A

Y

R

E

K

G

H

H

K

Y

Y

A

G

I

V

G

G

A

A

F

F

N
|
N

V

V

N

N

M

M

E

E

I

Q

V

I

Q

Q

G

G

I

I

T

M

E

K

A

A

A

V

K

V

E

Q

L

L

N

K

A

S

P

P

L

V

I

I

L

L

Q

Q

C

C

S
|
S

A

R

G

G

A

A

R

L

K

K

Y

Y

A

S

K

D

H

M

Q

I

Y

Y

L

L

V

K

H

K

L

L

V

C

Q

E

A

A

I

L

E

E

D

K

-

H

T

P

G

D

L

I

P

P

I

I

A

C

L

I

H

H

L

L

D
|
D

H
|
H

G

G

A

D

D

T

F

L

E

E

T

S

C

V

K

K

S

M

C

A

I

I

D

D

G

L

G

G

F

F

T

S

S

S

V

V

M

M

F

I

D

D

G

A

S

S

H

H

Y

H

S

P

F

F

K

D

E

E

N

N

I

V

E

R

K

I

T

T

K

K

E

E

V

V

E

A

Y

Y

A

A

H

H

A

A

H

R

G

G

V

V

V

S

V

V

E

E

A

A

E

E

L

L

G

G

Q

T

L

L

A

G

G

G

I

I

E

E

D

Q

D

N

V

T

K

-

V

-

A

-

H

-

E

-

A

V

H

Q

Y

L

T

T

D

E

P

P

N

Q

E

D

V

A

E

K

F

F

V

V

K

E

E

L

T

T

G

G

V

V

D

D

S

A

L

L

A

A

I

V

A

A

I

I

G

G

T

T

S

S

H
|
H

G
|
G

A

A

Y

Y

K
|
K

F

F

T

K

P

S

D

E

Q

S

C

D

T

I

R

R

N

-

E

-

Q

-

G

-

I

-

L

-

V

-

P

-

L

L

R

A

F

I

D

D

I

R

L

V

E

K

E

T

V

I

A

S

K

D

R

-

L

L

P

T

N

G

F

I

P

P

I

L

V

V

L

M

H
|
H

G
|
G

A

S

S
|
S

S

S

V

V

P

P

Q

K

D

D

F

V

V

K

K

D

I

M

I

I

N

N

E

K

N

Y

G

G

H

K

M

M

P

P

D

D

A

A

V

V

G

G

V

V

P

P

E

I

D

E

Q

S

L

I

R

V

K

H

A

A

A

I

S

G

M

E

A

G

V

V

C

C

K

K

I

I

N
|
N

I

V

D
|
D

S
|
S

D

D

L

S

R
|
R

L

M

A

A

M

M

T

T

A

G

G

A

I

I

R

R

K

K

H

V

F

F

N

V

D

E

H

H

P

P

D

E

H

K

F

F

D

D

P

P

R

R

Q

D

Y

Y

V

L

A

G

D

P

G

G

R

R

T

D

N

A

I

I

K

T

A

E

I

M

V

L

S

I

H

P

K

K

I

I

K

K

binding 1,6-di-O-phosphono-D-tagatose: N23 (= N24), S49 (= S50), D82 (= D82), H174 (= H180), G175 (= G181), K178 (= K184), H206 (= H223), G207 (= G224), S209 (= S226), N249 (= N266), D251 (= D268), S252 (= S269), R255 (= R272)
binding zinc ion: H83 (= H83), H174 (= H180), H206 (= H223)

3gb6A Structure of giardia fructose-1,6-biphosphate aldolase d83a mutant in complex with fructose-1,6-bisphosphate (see paper)
50% identity, 97% coverage: 2:312/321 of query aligns to 1:294/318 of 3gb6A

query
sites
3gb6A

P

P

L

L

V

C

T

T

T

L

K

R

E

Q

M

M

F

L

K

G

K

E

A

A

Y

R

E

K

G

H

H

K

Y

Y

A

G

I

V

G

G

A

A

F

F

N
|
N

V

V

N

N

M

M

E

E

I

Q

V

I

Q

Q

G

G

I

I

T

M

E

K

A

A

A

V

K

V

E

Q

L

L

N

K

A

S

P

P

L

V

I

I

L

L

Q

Q

C

C

S
|
S

A

R

G

G

A

A

R

L

K

K

Y

Y

A

S

K

D

H

M

Q

I

Y

Y

L

L

V

K

H

K

L

L

V

C

Q

E

A

A

I

L

E

E

D

K

-

H

T

P

G

D

L

I

P

P

I

I

A

C

L

I

H

H

L

L

D

A

H

H

G

G

A

D

D

T

F

L

E

E

T

S

C

V

K

K

S

M

C

A

I

I

D

D

G

L

G

G

F

F

T

S

S

S

V

V

M

M

F

I

D

D

G

A

S

S

H

H

Y

H

S

P

F

F

K

D

E

E

N

N

I

V

E

R

K

I

T

T

K

K

E

E

V

V

E

A

Y

Y

A

A

H

H

A

A

H

R

G

G

V

V

V

S

V

V

E

E

A

A

E

E

L

L

G

G

Q

T

L

L

A

G

G

G

I

I

E

E

D

N

D

T

V

V

K

Q

V

L

A

-

H

-

E

-

A

-

H

-

Y

-

T

T

D

E

P

P

N

Q

E

D

V

A

E

K

F

F

V

V

K

E

E

L

T

T

G

G

V

V

D

D

S

A

L

L

A

A

I

V

A

A

I

I

G

G

T

T

S

S

H
|
H

G
|
G

A

A

Y

Y

K
|
K

F

F

T

K

P

S

D

E

Q

S

C

D

T

I

R

R

N

-

E

-

Q

-

G

-

I

-

L

-

V

-

P

-

L

L

R

A

F

I

D

D

I

R

L

V

E

K

E

T

V

I

A

S

K

D

R

-

L

L

P

T

N

G

F

I

P

P

I

L

V

V

L

M

H
|
H

G
|
G

A

S

S
|
S

S

S

V

V

P

P

Q

K

D

D

F

V

V

K

K

D

I

M

I

I

N

N

E

K

N

Y

G

G

H

K

M

M

P

P

D

D

A

A

V

V

G

G

V

V

P

P

E

I

D

E

Q

S

L

I

R

V

K

H

A

A

A

I

S

G

M

E

A

G

V

V

C

C

K

K

I

I

N
|
N

I

V

D
|
D

S
|
S

D

D

L

S

R
|
R

L

M

A

A

M

M

T

T

A

G

G

A

I

I

R

R

K

K

H

V

F

F

N

V

D

E

H

H

P

P

D

E

H

K

F

F

D

D

P

P

R

R

Q

D

Y

Y

V

L

A

G

D

P

G

G

R

R

T

D

N

A

I

I

K

T

A

E

I

M

V

L

S

I

H

P

K

K

I

I

K

K

binding 1,6-di-O-phosphono-D-fructose: N23 (= N24), S49 (= S50), H173 (= H180), G174 (= G181), K177 (= K184), H205 (= H223), G206 (= G224), S208 (= S226), N248 (= N266), D250 (= D268), S251 (= S269), R254 (= R272)

3ohiA Structure of giardia fructose-1,6-biphosphate aldolase in complex with 3-hydroxy-2-pyridone (see paper)
49% identity, 97% coverage: 2:312/321 of query aligns to 1:295/319 of 3ohiA

query
sites
3ohiA

P

P

L

L

V

C

T

T

T

L

K

R

E

Q

M

M

F

L

K

G

K

E

A

A

Y

R

E

K

G

H

H

K

Y

Y

A

G

I

V

G

G

A

A

F

F

N

N

V

V

N

N

M

M

E

E

I

Q

V

I

Q

Q

G

G

I

I

T

M

E

K

A

A

A

V

K

V

E

Q

L

L

N

K

A

S

P

P

L

V

I

I

L

L

Q

Q

C

C

S
|
S

A

R

G

G

A

A

R

L

K

K

Y

Y

A

S

K

D

H

M

Q

I

Y

Y

L

L

V

K

H

K

L

L

V

C

Q

E

A

A

I

L

E

E

D

K

-

H

T

P

G

D

L

I

P

P

I

I

A

C

L

I

H

H

L

L

D
|
D

H
|
H

G

G

A

D

D

T

F

L

E

E

T

S

C

V

K

K

S

M

C

A

I

I

D

D

G

L

G

G

F

F

T

S

S

S

V

V

M

M

F

I

D

D

G

A

S

S

H

H

Y

H

S

P

F

F

K

D

E

E

N

N

I

V

E

R

K

I

T

T

K

K

E

E

V

V

E

A

Y

Y

A

A

H

H

A

A

H

R

G

S

V

V

V

S

V

V

E

E

A

A

E

E

L

L

G

G

Q

T

L

L

A

G

G

G

I

I

E

E

D

Q

D

N

V

T

K

-

V

-

A

-

H

-

E

-

A

V

H

Q

Y

L

T

T

D

E

P

P

N

Q

E

D

V

A

E

K

F

F

V

V

K

E

E

L

T

T

G

G

V

V

D

D

S

A

L

L

A

A

I

V

A

A

I

I

G

G

T

T

S

S

H
|
H

G
|
G

A

A

Y

Y

K
|
K

F

F

T

K

P

S

D

E

Q

S

C

D

T

I

R

R

N

-

E

-

Q

-

G

-

I

-

L

-

V

-

P

-

L

L

R

A

F

I

D

D

I

R

L

V

E

K

E

T

V

I

A

S

K

D

R

-

L

L

P

T

N

G

F

I

P

P

I

L

V

V

L

M

H
|
H

G
|
G

A

S

S
|
S

S

S

V

V

P

P

Q

K

D

D

F

V

V

K

K

D

I

M

I

I

N

N

E

K

N

Y

G

G

H

K

M

M

P

P

D

D

A

A

V

V

G

G

V

V

P

P

E

I

D

E

Q

S

L

I

R

V

K

H

A

A

A

I

S

G

M

E

A

G

V

V

C

C

K

K

I

I

N
|
N

I

V

D
|
D

S
|
S

D

D

L

S

R
|
R

L

M

A

A

M

M

T

T

A

G

G

A

I

I

R

R

K

K

H

V

F

F

N

V

D

E

H

H

P

P

D

E

H

K

F

F

D

D

P

P

R

R

Q

D

Y

Y

V

L

A

G

D

P

G

G

R

R

T

D

N

A

I

I

K

T

A

E

I

M

V

L

S

I

H

P

K

K

I

I

K

K

binding ({3-hydroxy-2-oxo-4-[2-(phosphonooxy)ethyl]pyridin-1(2H)-yl}methyl)phosphonic acid: S49 (= S50), D82 (= D82), H83 (= H83), H174 (= H180), G175 (= G181), K178 (= K184), G207 (= G224), S209 (= S226), N249 (= N266), D251 (= D268), S252 (= S269), R255 (= R272)
binding zinc ion: H83 (= H83), H174 (= H180), H206 (= H223)

2isvB Structure of giardia fructose-1,6-biphosphate aldolase in complex with phosphoglycolohydroxamate (see paper)
48% identity, 97% coverage: 2:312/321 of query aligns to 1:284/307 of 2isvB

query
sites
2isvB

P

P

L

L

V

C

T

T

T

L

K

R

E

Q

M

M

F

L

K

G

K

E

A

A

Y

R

E

K

G

H

H

K

Y

Y

A

G

I

V

G

G

A

A

F

F

N

N

V

V

N

N

M

M

E

E

I

Q

V

I

Q

Q

G

G

I

I

T

M

E

K

A

A

A

V

K

V

E

Q

L

L

N

K

A

S

P

P

L

V

I

I

L

L

Q

Q

C

C

S

S

A

R

G

G

A

A

R

L

K

K

Y

Y

A

S

K

D

H

M

Q

I

Y

Y

L

L

V

K

H

K

L

L

V

C

Q

E

A

A

I

L

E

E

D

K

-

H

T

P

G

D

L

I

P

P

I

I

A

C

L

I

H

H

L

L

D
|
D

H
|
H

G

G

A

D

D

T

F

L

E

E

T

S

C

V

K

K

S

M

C

A

I

I

D

D

G

L

G

G

F

F

T

S

S

S

V

V

M

M

F

I

D

D

G

A

S

S

H

H

Y

H

S

P

F

F

K

D

E

E

N

N

I

V

E

R

K

I

T

T

K

K

E

E

V

V

E

A

Y

Y

A

A

H

H

A

A

H

R

G

S

V

V

V

S

V

V

E

E

A

A

E

E

L

L

G

G

Q

Q

L

-

A

-

G

-

I

-

E

-

D

-

V

-

K

-

V

-

A

-

H

N

E

T

A

V

H

Q

Y

L

T

T

D

E

P

P

N

Q

E

D

V

A

E

K

F

F

V

V

K

E

E

L

T

T

G

G

V

V

D

D

S

A

L

L

A

A

I

V

A

A

I

I

G

G

T

T

S

S

H
|
H

G
|
G

A

A

Y

Y

K
|
K

F

F

T

I

P

R

D

-

Q

-

C

-

T

-

R

-

N

-

E

-

Q

-

G

-

I

-

L

-

V

-

P

-

L

L

R

A

F

I

D

D

I

R

L

V

E

K

E

T

V

I

A

S

K

D

R

-

L

L

P

T

N

G

F

I

P

P

I

L

V

V

L

M

H
|
H

G
|
G

A

S

S
|
S

S

S

V

V

P

P

Q

K

D

D

F

V

V

K

K

D

I

M

I

I

N

N

E

K

N

Y

G

G

H

K

M

M

P

P

D

D

A

A

V

V

G

G

V

V

P

P

E

I

D

E

Q

S

L

I

R

V

K

H

A

A

A

I

S

G

M

E

A

G

V

V

C

C

K

K

I

I

N
|
N

I

V

D
|
D

S
|
S

D

D

L

S

R

R

L

M

A

A

M

M

T

T

A

G

G

A

I

I

R

R

K

K

H

V

F

F

N

V

D

E

H

H

P

P

D

E

H

K

F

F

D

D

P

P

R

R

Q

D

Y

Y

V

L

A

G

D

P

G

G

R

R

T

D

N

A

I

I

K

T

A

E

I

M

V

L

S

I

H

P

K

K

I

I

K

K

binding phosphoglycolohydroxamic acid: D82 (= D82), H168 (= H180), G169 (= G181), K172 (= K184), H195 (= H223), G196 (= G224), S198 (= S226), N238 (= N266), D240 (= D268), S241 (= S269)
binding zinc ion: H83 (= H83), H168 (= H180), H195 (= H223)

2isvA Structure of giardia fructose-1,6-biphosphate aldolase in complex with phosphoglycolohydroxamate (see paper)
47% identity, 97% coverage: 2:312/321 of query aligns to 1:275/298 of 2isvA

query
sites
2isvA

P

P

L

L

V

C

T

T

T

L

K

R

E

Q

M

M

F

L

K

G

K

E

A

A

Y

R

E

K

G

H

H

K

Y

Y

A

G

I

V

G

G

A

A

F

F

N

N

V

V

N

N

M

M

E

E

I

Q

V

I

Q

Q

G

G

I

I

T

M

E

K

A

A

A

V

K

V

E

Q

L

L

N

K

A

S

P

P

L

V

I

I

L

L

Q

Q

C

C

S

S

A

R

G

G

A

A

R

L

K

K

Y

Y

A

S

K

D

H

M

Q

I

Y

Y

L

L

V

K

H

K

L

L

V

C

Q

E

A

A

I

L

E

E

D

K

-

H

T

P

G

D

L

I

P

P

I

I

A

C

L

I

H

H

L

L

D
|
D

H
|
H

G

G

A

D

D

T

F

L

E

E

T

S

C

V

K

K

S

M

C

A

I

I

D

D

G

L

G

G

F

F

T

S

S

S

V

V

M

M

F

I

D

D

G

A

S

S

H

H

Y

H

S

P

F

F

K

D

E

E

N

N

I

V

E

R

K

I

T

T

K

K

E

E

V

V

E

A

Y

Y

A

A

H

H

A

A

H

R

G

S

V

V

V

S

V

V

E

E

A

A

E

E

L

L

G

G

Q

-

L

-

A

-

G

-

I

-

E

-

D

-

V

-

K

-

V

-

A

-

H

-

E

-

A

-

H

-

Y

L

T

T

D

E

P

P

N

Q

E

D

V

A

E

K

F

F

V

V

K

E

E

L

T

T

G

G

V

V

D

D

S

A

L

L

A

A

I

V

A

A

I

I

G

G

T

T

S

S

H
|
H

G

G

A

A

Y

R

K

-

F

-

T

-

P

-

D

-

Q

-

C

-

T

-

R

-

N

-

E

-

Q

-

G

-

I

-

L

-

V

-

P

-

L

L

R

A

F

I

D

D

I

R

L

V

E

K

E

T

V

I

A

S

K

D

R

-

L

L

P

T

N

G

F

I

P

P

I

L

V

V

L

M

H
|
H

G
|
G

A

S

S
|
S

S

S

V

V

P

P

Q

K

D

D

F

V

V

K

K

D

I

M

I

I

N

N

E

K

N

Y

G

G

H

K

M

M

P

P

D

D

A

A

V

V

G

G

V

V

P

P

E

I

D

E

Q

S

L

I

R

V

K

H

A

A

A

I

S

G

M

E

A

G

V

V

C

C

K

K

I

I

N
|
N

I

V

D
|
D

S
|
S

D

D

L

S

R

R

L

M

A

A

M

M

T

T

A

G

G

A

I

I

R

R

K

K

H

V

F

F

N

V

D

E

H

H

P

P

D

E

H

K

F

F

D

D

P

P

R

R

Q

D

Y

Y

V

L

A

G

D

P

G

G

R

R

T

D

N

A

I

I

K

T

A

E

I

M

V

L

S

I

H

P

K

K

I

I

K

K

binding phosphoglycolohydroxamic acid: D82 (= D82), H163 (= H180), H186 (= H223), G187 (= G224), S189 (= S226), N229 (= N266), D231 (= D268), S232 (= S269)
binding zinc ion: H83 (= H83), H163 (= H180), H186 (= H223)

1rv8B Class ii fructose-1,6-bisphosphate aldolase from thermus aquaticus in complex with cobalt (see paper)
45% identity, 99% coverage: 3:321/321 of query aligns to 2:305/305 of 1rv8B

query
sites
1rv8B

L

L

V

V

T

T

T

G

K

L

E

E

M

I

F

L

K

K

A

A

Y

R

E

E

G

E

H

G

Y

Y

A

G

I

V

G

G

A

A

F

F

N

N

V

V

N

N

M

M

E

E

I

F

V

L

Q

Q

G

A

I

V

T

L

E

E

A

A

K

E

E

E

L

Q

N

R

A

S

P

P

L

V

I

I

L

L

Q

A

C

L

S

S

A

E

G

G

A

A

R

M

K

K

Y

Y

A

G

K

G

H

R

Q

A

Y

L

L

T

V

L

H

M

L

A

V

V

Q

E

A

L

A

A

I

-

E

K

D

E

T

A

G

R

L

V

P

P

I

V

A

A

L

V

H

H

L

L

D
|
D

H
|
H

G

G

A

S

D

S

F

Y

E

E

T

S

C

V

K

L

S

R

C

A

I

L

D

R

G

A

G

G

F

F

T

T

S

S

V

V

M

M

F

I

D

D

G

K

S

S

H

H

Y

E

S

D

F

F

K

E

E

T

N

N

I

V

E

R

K

E

T

T

K
x
R

E

R

V

V

E

E

Y

A

A

A

H
|
H

A

A

H

V

G

G

V

V

V

T

V

V

E

E

A

A

E
|
E

L

L

G

G

Q

R

L

L

A

A

G

G

I

I

E
|
E

D

E

D

H

V

V

K

A

V

V

A

D

A

E

H

K

E

D

A

A

H

L

Y

L

T

T

D

N

P

P

N

E

E

E

V

A

E

R

E

I

F

F

V

M

K

E

E

R

T

T

G

G

V

A

D

D

S

Y

L

L

A

A

I

V

A

A

I

I

G

G

T

T

S

S

H
|
H

G

G

A

A

Y

Y

K

K

F

G

T

K

P

G

D

R

Q

-

C

-

T

-

R

-

N

-

E

-

Q

-

G

-

I

-

L

-

V

-

P

-

P

P

P

F

L

I

R

D

F

H

D

A

I

R

L

L

E

E

E

R

V

I

A

A

K

R

R

L

L

V

P

P

N

A

F

-

P

P

I

L

V

V

L

L

H
|
H

G

G

A

A

S
|
S

S

A

V

V

P

P

Q

P

D

E

F

L

V

V

K

E

I

R

I

F

N

R

E

A

N

S

G

G

H

E

M

I

P

G

D

E

A

A

V

A

G

G

V

I

P

H

E

P

D

E

Q

D

L

I

R

K

K

K

A

A

A

I

S

S

M

L

A

G

V

I

C

A

K

K

I

I

N
|
N

I

T

D
|
D

S
x
T

D

D

L

L

R

R

L

L

A

A

M

F

T

T

A

A

G

L

I

I

R

R

K

E

H

A

F

L

N

N

D

K

H

N

P

P

D

K

H

E

F

F

D

D

P

P

R

R

Q

K

Y

Y

V

L

A

G

D

P

G

A

R

R

T

E

N

A

I

V

K

K

A

E

I

V

V

V

S

K

H

S

K

R

I

M

K

-

E

E

V

L

L

F

G

G

C

S

D

V

G

G

K

R

A

A

active site: D80 (= D82), H81 (= H83), E140 (= E142), H178 (= H180), H208 (= H223), N251 (= N266)
binding cobalt (ii) ion: H81 (= H83), E132 (= E134), H178 (= H180), H208 (= H223)
binding sulfate ion: R116 (≠ K118), H123 (= H125), S211 (= S226), D253 (= D268), T254 (≠ S269)

3n9sA Class ii fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with n-(4-hydroxybutyl)- glycolohydroxamic acid bis- phosphate, a competitive inhibitor (see paper)
43% identity, 99% coverage: 3:320/321 of query aligns to 2:306/307 of 3n9sA

query
sites
3n9sA

L

L

V

V

T

K

T

G

K

N

E

E

M

I

F

L

K

L

K

K

A

A

Y

H

E

K

G

E

H

G

Y

Y

A

G

I

V

G

G

A

A

F

F

N
|
N

V

F

N

V

N

N

M

F

E

E

I

M

V

L

Q

N

G

A

I

I

T

F

E

E

A

A

A

G

K

N

E

E

L

E

N

N

A

S

P

P

L

L

I

F

L

I

Q

Q

C

A

S
|
S

A

E

G

G

A

A

R

I

K

K

Y

Y

A

M

K

G

H

I

Q

D

Y

M

L

A

V

V

H

G

L

M

V

V

Q

K

A

I

A

M

I
x
C

E
|
E

D

R

T

Y

G

P

-

H

L

I

P

P

I

V

A

A

L

L

H

H

L

L

D
|
D

H
|
H

G

G

A

T

D

T

F

F

E

E

T

S

C

C

K

E

S

K

C

A

I

V

D

K

G

A

G

G

F

F

T

T

S

S

V

V

M

M

F

I

D
|
D

G

A

S
|
S

H

H

Y

H

S

A

F

F

K

E

E

E

N

N

I

L

E

E

K

L

T

T

K

S

E

K

V

V

E

K

Y

M

A

A

H

H

A

N

H

A

G

G

V

V

V

S

V

V

E

E

A

A

E
|
E

L

L

G
|
G

Q

R

L

L

A

M

G

G

I

I

E

E

D

D

D

N

V

I

K

S

V

V

A

D

A

E

H

K

E

D

A

A

H

V

Y

L

T

V

D

N

P

P

N

K

E

E

V

A

E

E

E

Q

F

F

V

V

K

K

E

E

T

S

G

Q

V

V

D

D

S

Y

L

L

A

A

I

P

A

A

I

I

G

G

T

T

S

S

H
|
H

G
|
G

A

A

Y

F

K
|
K

F

F

T

K

P

G

D

E

Q

-

C

-

T

-

R

-

N

-

E

-

Q

-

G

-

I

-

L

-

V

-

P

-

P

P

P

K

L

L

R

D

F

F

D

E

I

R

L

L

E

Q

E

E

V

V

A

-

K

K

R

R

L

L

P

T

N

N

F

I

P

P

I

L

V

V

L

L

H
|
H

G
|
G

A

A

S
|
S

S

A

V

I

P

P

Q

D

D

N

F

V

V

R

K

K

I

S

I

Y

N

L

E

D

N
x
A

G

G

H

D

M

L

P

K

D

G

A

S

V

K

G

G

V

V

P

P

E

F

D

E

Q

F

L

L

R

Q

K

E

A

S

A

V

S

K

M

G

A

G

V

I

C

N

K

K

I

V

N
|
N

I

T

D
|
D

S
x
T

D

D

L

L

R

R

L

I

A

A

M

F

T

I

A

A

G

E

I

V

R

R

K

K

H

V

F

A

N

N

D

E

H

D

P

K

D

S

H

Q

F

F

D

D

P

L

R

R

Q

K

Y

F

V

F

A

S

D

P

G

A

R

Q

T

L

N

A

I

L

K

K

A

N

I

V

V

V

S

K

H

E

K

R

I

M

K

K

E

-

V

L

L

L

G

G

C

S

D

A

G

N

K

K

active site: C69 (≠ I70), E70 (= E71), G136 (= G136), H180 (= H180), A226 (≠ N239), N253 (= N266)
binding calcium ion: D104 (= D104), S106 (= S106), E134 (= E134)
binding 4-{hydroxy[(phosphonooxy)acetyl]amino}butyl dihydrogen phosphate: N23 (= N24), S49 (= S50), D82 (= D82), H83 (= H83), H180 (= H180), G181 (= G181), K184 (= K184), H210 (= H223), G211 (= G224), S213 (= S226), N253 (= N266), D255 (= D268), T256 (≠ S269)
binding zinc ion: H83 (= H83), H180 (= H180), H210 (= H223)

3q94A The crystal structure of fructose 1,6-bisphosphate aldolase from bacillus anthracis str. 'Ames ancestor'
42% identity, 100% coverage: 1:321/321 of query aligns to 1:285/285 of 3q94A

query
sites
3q94A

M

M

P

P

L

L

V

V

T

S

T

M

K

K

E

E

M

M

F

L

K

N

K

K

A

A

Y

L

E

E

G

G

H

K

Y

Y

A

A

I

V

G

G

A

Q

F

F

N

N

V

M

N

N

M

L

E

E

I

W

V

T

Q

Q

G

A

I

I

T

L

E

A

A

A

K

E

E

E

L

E

N

K

A

S

P

P

L

V

I

I

L

L

Q

G

C

V

S

S

A

E

G

G

A

A

-

A

R

R

K

H

Y

M

A

T

K

G

H

F

Q

K

Y

T

L

V

V

V

H

A

L

M

V

V

Q

K

A

A

A

L

I

I

E

E

D

E

T

M

G

N

L

I

-

T

-

V

P

P

I

V

A

A

L

I

H

H

L

L

D
|
D

H
|
H

G

G

A

S

D

S

F

F

E

E

T

K

C

C

K

K

S

E

C

A

I

I

D

D

G

A

G

G

F

F

T

T

S

S

V

V

M

M

F

I

D

D

G

A

S

S

H

H

Y

H

S

P

F

F

K
x
E

E

E

N

N

I

V

E

E

K

T

T

T

K

K

E

K

V

V

E

E

Y

Y

A

A

H

H

A

A

H

R

G

N

V

V

V

S

V

V

E

E

A

A

E

E

L

L

G

G

Q

T

L

V

A

G

G

G

I

Q

E
|
E

D

D

V

-

K

-

V

V

A

I

A

A

H

E

E

G

A

V

H

I

Y

Y

T

A

D

D

P

P

N

A

E

E

V

C

E

K

E
x
H

F

L

V

V

K

E

E

A

T

T

G

G

V

I

D

D

S

C

L

L

A

A

I

P

A

A

I

L

G

G

T

S

S

V

H
|
H

G

G

A

P

Y

Y

K

K

F

G

T

E

P

P

D

N

Q

-

C

-

T

-

R

-

N

-

E

-

Q

-

G

-

I

-

L

-

V

-

P

-

L

L

R

G

F

F

D

A

I

E

L

M

E

E

E

Q

V

V

A

-

K

R

R

D

L

F

P

T

N

G

F

V

P

P

I

L

V

V

L

L

H
|
H

G

G

A

G

S

T

S

-

V

-

P

-

Q

-

D

-

F

-

V

-

K

-

I

-

N

-

E

-

N

-

G

-

H

-

M

-

P

-

D

-

A

-

V

-

G

G

V

I

P

P

E

T

D

A

Q

D

L

I

R

E

K

K

A

A

A

I

S

S

M

L

A

G

V

T

C

S

K

K

I

I

N
|
N

I

V

D

N

S

T

D
x
E

L

N

R

Q

L

I

A
x
E

M

F

T

T

A

K

G

A

I

V

R

R

K

E

H

V

F

L

N

N

D

K

H

D

P

Q

D

E

H

V

F

Y

D

D

P

P

R

R

Q

K

Y

F

V

I

A

G

D

P

G

G

R

R

T

D

N

A

I

I

K

K

A

A

I

T

V

V

S

I

H

G

K

K

I

I

K

R

E

E

V

-

L

F

G

G

C

S

D

N

G

G

K

K

A

A

active site: D85 (= D82), H86 (= H83), E145 (= E142), H181 (= H180), H209 (= H223), N231 (= N266)
binding zinc ion: H86 (= H83), E114 (≠ K111), H163 (≠ E162), H181 (= H180), H209 (= H223), E235 (≠ D270), E239 (≠ A274)

3n9rA Class ii fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with n-(4-hydroxybutyl)-phosphoglycolohydroxamic acid, a competitive inhibitor (see paper)
42% identity, 99% coverage: 3:320/321 of query aligns to 2:296/297 of 3n9rA

query
sites
3n9rA

L

L

V

V

T

K

T

G

K

N

E

E

M

I

F

L

K

L

K

K

A

A

Y

H

E

K

G

E

H

G

Y

Y

A

G

I

V

G

G

A

A

F

F

N

N

V

F

N

V

N

N

M

F

E

E

I

M

V

L

Q

N

G

A

I

I

T

F

E

E

A

A

A

G

K

N

E

E

L

E

N

N

A

S

P

P

L

L

I

F

L

I

Q

Q

C

A

S

S

A

E

G

G

A

A

R

I

K

K

Y

Y

A

M

K

G

H

I

Q

D

Y

M

L

A

V

V

H

G

L

M

V

V

Q

K

A

I

A

M

I
x
C

E
|
E

D

R

T

Y

G

P

-

H

L

I

P

P

I

V

A

A

L

L

H

H

L

L

D

D

H
|
H

G

G

A

T

D

T

F

F

E

E

T

S

C

C

K

E

S

K

C

A

I

V

D

K

G

A

G

G

F

F

T

T

S

S

V

V

M

M

F

I

D

D

G

A

S

S

H

H

Y

H

S

A

F

F

K

E

E

E

N

N

I

L

E

E

K

L

T

T

K

S

E

K

V

V

E

K

Y

M

A

A

H

H

A

N

H

A

G

G

V

V

V

S

V

V

E

E

A

A

E

E

L

L

G
|
G

Q

R

L

L

A

M

G

G

I

I

E

-

D

-

V

-

K

-

V

-

A

-

H

-

E

-

A

A

H

V

Y

L

T

V

D

N

P

P

N

K

E

E

V

A

E

E

E

Q

F

F

V

V

K

K

E

E

T

S

G

Q

V

V

D

D

S

Y

L

L

A

A

I

P

A

A

I

I

G

G

T

T

S

S

H
|
H

G
|
G

A

A

Y

F

K
|
K

F

F

T

K

P

G

D

E

Q

-

C

-

T

-

R

-

N

-

E

-

Q

-

G

-

I

-

L

-

V

-

P

-

P

P

P

K

L

L

R

D

F

F

D

E

I

R

L

L

E

Q

E

E

V

V

A

-

K

K

R

R

L

L

P

T

N

N

F

I

P

P

I

L

V

V

L

L

H
|
H

G
|
G

A

A

S
|
S

S

A

V

I

P

P

Q

D

D

N

F

V

V

R

K

K

I

S

I

Y

N

L

E

D

N
x
A

G

G

H

D

M

L

P

K

D

G

A

S

V

K

G

G

V

V

P

P

E

F

D

E

Q

F

L

L

R

Q

K

E

A

S

A

V

S

K

M

G

A

G

V

I

C

N

K

K

I

V

N
|
N

I

T

D
|
D

S
x
T

D

D

L

L

R

R

L

I

A

A

M

F

T

I

A

A

G

E

I

V

R

R

K

K

H

V

F

A

N

N

D

E

H

D

P

K

D

S

H

Q

F

F

D

D

P

L

R

R

Q

K

Y

F

V

F

A

S

D

P

G

A

R

Q

T

L

N

A

I

L

K

K

A

N

I

V

V

V

S

K

H

E

K

R

I

M

K

K

E

-

V

L

L

L

G

G

C

S

D

A

G

N

K

K

active site: C69 (≠ I70), E70 (= E71), G136 (= G136), H170 (= H180), A216 (≠ N239), N243 (= N266)
binding 2-[hydroxy(4-hydroxybutyl)amino]-2-oxoethyl dihydrogen phosphate: H83 (= H83), H170 (= H180), G171 (= G181), K174 (= K184), H200 (= H223), G201 (= G224), S203 (= S226), N243 (= N266), D245 (= D268), T246 (≠ S269)
binding zinc ion: H83 (= H83), H170 (= H180), H200 (= H223)

3c56A Class ii fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with n-(3-hydroxypropyl)-glycolohydroxamic acid bisphosphate, a competitive inhibitor (see paper)
42% identity, 99% coverage: 3:320/321 of query aligns to 2:296/297 of 3c56A

query
sites
3c56A

L

L

V

V

T

K

T

G

K

N

E

E

M

I

F

L

K

L

K

K

A

A

Y

H

E

K

G

E

H

G

Y

Y

A

G

I

V

G

G

A

A

F

F

N
|
N

V

F

N

V

N

N

M

F

E

E

I

M

V

L

Q

N

G

A

I

I

T

F

E

E

A

A

A

G

K

N

E

E

L

E

N

N

A

S

P

P

L

L

I

F

L

I

Q

Q

C

A

S
|
S

A

E

G

G

A

A

R

I

K

K

Y

Y

A

M

K

G

H

I

Q

D

Y

M

L

A

V

V

H

G

L

M

V

V

Q

K

A

I

A

M

I
x
C

E
|
E

D

R

T

Y

G

P

-

H

L

I

P

P

I

V

A

A

L

L

H

H

L

L

D
|
D

H
|
H

G

G

A

T

D

T

F

F

E

E

T

S

C

C

K

E

S

K

C

A

I

V

D

K

G

A

G

G

F

F

T

T

S

S

V

V

M

M

F

I

D

D

G

A

S

S

H

H

Y

H

S

A

F

F

K

E

E

E

N

N

I

L

E

E

K

L

T

T

K

S

E

K

V

V

E

K

Y

M

A

A

H

H

A

N

H

A

G

G

V

V

V

S

V

V

E

E

A

A

E

E

L

L

G
|
G

Q

R

L

L

A

M

G

G

I

I

E

-

D

-

V

-

K

-

V

-

A

-

H

-

E

-

A

A

H

V

Y

L

T

V

D

N

P

P

N

K

E

E

V

A

E

E

E

Q

F

F

V

V

K

K

E

E

T

S

G

Q

V

V

D

D

S

Y

L

L

A

A

I

P

A

A

I

I

G

G

T

T

S

S

H
|
H

G

G

A

A

Y

F

K
|
K

F

F

T

K

P

G

D

E

Q

-

C

-

T

-

R

-

N

-

E

-

Q

-

G

-

I

-

L

-

V

-

P

-

P

P

P

K

L

L

R

D

F

F

D

E

I

R

L

L

E

Q

E

E

V

V

A

-

K

K

R

R

L

L

P

T

N

N

F

I

P

P

I

L

V

V

L

L

H
|
H

G
|
G

A

A

S
|
S

S

A

V

I

P

P

Q

D

D

N

F

V

V

R

K

K

I

S

I

Y

N

L

E

D

N
x
A

G

G

H

D

M

L

P

K

D

G

A

S

V

K

G

G

V

V

P

P

E

F

D

E

Q

F

L

L

R

Q

K

E

A

S

A

V

S

K

M

G

A

G

V

I

C

N

K

K

I

V

N
|
N

I

T

D
|
D

S
x
T

D

D

L

L

R
|
R

L

I

A

A

M

F

T

I

A

A

G

E

I

V

R

R

K

K

H

V

F

A

N

N

D

E

H

D

P

K

D

S

H

Q

F

F

D

D

P

L

R

R

Q

K

Y

F

V

F

A

S

D

P

G

A

R

Q

T

L

N

A

I

L

K

K

A

N

I

V

V

V

S

K

H

E

K

R

I

M

K

K

E

-

V

L

L

L

G

G

C

S

D

A

G

N

K

K

active site: C69 (≠ I70), E70 (= E71), G136 (= G136), H170 (= H180), A216 (≠ N239), N243 (= N266)
binding 3-{hydroxy[(phosphonooxy)acetyl]amino}propyl dihydrogen phosphate: N23 (= N24), S49 (= S50), D82 (= D82), H170 (= H180), K174 (= K184), G201 (= G224), S203 (= S226), N243 (= N266), D245 (= D268), T246 (≠ S269), R249 (= R272)
binding zinc ion: H83 (= H83), H170 (= H180), H200 (= H223)

3c52A Class ii fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with phosphoglycolohydroxamic acid, a competitive inhibitor (see paper)
42% identity, 99% coverage: 3:320/321 of query aligns to 2:295/296 of 3c52A

query
sites
3c52A

L

L

V

V

T

K

T

G

K

N

E

E

M

I

F

L

K

L

K

K

A

A

Y

H

E

K

G

E

H

G

Y

Y

A

G

I

V

G

G

A

A

F

F

N

N

V

F

N

V

N

N

M

F

E

E

I

M

V

L

Q

N

G

A

I

I

T

F

E

E

A

A

A

G

K

N

E

E

L

E

N

N

A

S

P

P

L

L

I

F

L

I

Q

Q

C

A

S

S

A

E

G

G

A

A

R

I

K

K

Y

Y

A

M

K

G

H

I

Q

D

Y

M

L

A

V

V

H

G

L

M

V

V

Q

K

A

I

A

M

I
x
C

E
|
E

D

R

T

Y

G

P

-

H

L

I

P

P

I

V

A

A

L

L

H

H

L

L

D
|
D

H
|
H

G

G

A

T

D

T

F

F

E

E

T

S

C

C

K

E

S

K

C

A

I

V

D

K

G

A

G

G

F

F

T

T

S

S

V

V

M

M

F

I

D
|
D

G

A

S
|
S

H

H

Y

H

S

A

F

F

K

E

E

E

N

N

I

L

E

E

K

L

T

T

K

S

E

K

V

V

E

K

Y

M

A

A

H

H

A

N

H

A

G

G

V

V

V

S

V

V

E

E

A

A

E
|
E

L

L

G
|
G

Q

R

L

L

A

M

G

G

I

I

E

V

D

-

V

-

K

-

V

-

A

-

H

-

E

-

A

-

H

-

Y

L

T

V

D

N

P

P

N

K

E

E

V

A

E

E

E

Q

F

F

V

V

K

K

E

E

T

S

G

Q

V

V

D

D

S

Y

L

L

A

A

I

P

A

A

I

I

G

G

T

T

S

S

H
|
H

G

G

A

A

Y

F

K
|
K

F

F

T

K

P

G

D

E

Q

-

C

-

T

-

R

-

N

-

E

-

Q

-

G

-

I

-

L

-

V

-

P

-

P

P

P

K

L

L

R

D

F

F

D

E

I

R

L

L

E

Q

E

E

V

V

A

-

K

K

R

R

L

L

P

T

N

N

F

I

P

P

I

L

V

V

L

L

H
|
H

G
|
G

A

A

S
|
S

S

A

V

I

P

P

Q

D

D

N

F

V

V

R

K

K

I

S

I

Y

N

L

E

D

N
x
A

G

G

H

D

M

L

P

K

D

G

A

S

V

K

G

G

V

V

P

P

E

F

D

E

Q

F

L

L

R

Q

K

E

A

S

A

V

S

K

M

G

A

G

V

I

C

N

K

K

I

V

N
|
N

I

T

D
|
D

S
x
T

D

D

L

L

R

R

L

I

A

A

M

F

T

I

A

A

G

E

I

V

R

R

K

K

H

V

F

A

N

N

D

E

H

D

P

K

D

S

H

Q

F

F

D

D

P

L

R

R

Q

K

Y

F

V

F

A

S

D

P

G

A

R

Q

T

L

N

A

I

L

K

K

A

N

I

V

V

V

S

K

H

E

K

R

I

M

K

K

E

-

V

L

L

L

G

G

C

S

D

A

G

N

K

K

active site: C69 (≠ I70), E70 (= E71), G136 (= G136), H169 (= H180), A215 (≠ N239), N242 (= N266)
binding calcium ion: D104 (= D104), S106 (= S106), E134 (= E134)
binding phosphoglycolohydroxamic acid: D82 (= D82), H83 (= H83), H169 (= H180), K173 (= K184), H199 (= H223), G200 (= G224), S202 (= S226), N242 (= N266), D244 (= D268), T245 (≠ S269)
binding zinc ion: H83 (= H83), H169 (= H180), H199 (= H223)

5uckA Class ii fructose-1,6-bisphosphate aldolase of helicobacter pylori with cleavage products (see paper)
41% identity, 99% coverage: 3:320/321 of query aligns to 2:290/291 of 5uckA

query
sites
5uckA

L

L

V

V

T

K

T

G

K

N

E

E

M

I

F

L

K

L

K

K

A

A

Y

H

E

K

G

E

H

G

Y

Y

A

G

I

V

G

G

A

A

F

F

N

N

V

F

N

V

N

N

M

F

E

E

I

M

V

L

Q

N

G

A

I

I

T

F

E

E

A

A

A

G

K

N

E

E

L

E

N

N

A

S

P

P

L

L

I

F

L

I

Q

Q

C

A

S
|
S

A

E

G

G

A

A

R

I

K

K

Y

Y

A

M

K

G

H

I

Q

D

Y

M

L

A

V

V

H

G

L

M

V

V

Q

K

A

I

A

M

I

C

E

E

D

R

T

Y

G

P

-

H

L

I

P

P

I

V

A

A

L

L

H

H

L

L

D
|
D

H
|
H

G

G

A

T

D

T

F

F

E

E

T

S

C

C

K

E

S

K

C

A

I

V

D

K

G

A

G

G

F

F

T

T

S

S

V

V

M

M

F

I

D

D

G

A

S

S

H

H

Y

H

S

A

F

F

K

E

E

E

N

N

I

L

E

E

K

L

T

T

K

S

E

K

V

V

E

K

Y

M

A

A

H

H

A

N

H

A

G

G

V

V

V

S

V

V

E

E

A

A

E
|
E

L

L

G

G

Q

R

L

L

A

-

G

-

I

-

E

-

D

-

V

-

K

-

V

-

A

-

H

-

E

-

A

-

H

-

Y

-

T

V

D

N

P

P

N

K

E

E

V

A

E

E

E

Q

F

F

V

V

K

K

E

E

T

S

G

Q

V

V

D

D

S

Y

L

L

A

A

I

P

A

A

I

I

G

G

T

T

S

S

H
|
H

G

G

A

A

Y

F

K

K

F

F

T

K

P

G

D

E

Q

-

C

-

T

-

R

-

N

-

E

-

Q

-

G

-

I

-

L

-

V

-

P

-

P

P

P

K

L

L

R

D

F

F

D

E

I

R

L

L

E

Q

E

E

V

V

A

-

K

K

R

R

L

L

P

T

N

N

F

I

P

P

I

L

V

V

L

L

H
|
H

G

G

A

A

S

S

S

A

V

I

P

P

Q

D

D

N

F

V

V

R

K

K

I

S

I

Y

N

L

E

D

N

A

G

G

H

D

M

L

P

K

D

G

A

S

V

K

G

G

V

V

P

P

E

F

D

E

Q

F

L

L

R

Q

K

E

A

S

A

V

S

K

M

G

A

G

V

I

C

N

K

K

I

V

N

N

I

T

D
|
D

S

T

D

D

L

L

R
|
R

L

I

A

A

M

F

T

I

A

A

G

E

I

V

R

R

K

K

H

V

F

A

N

N

D

E

H

D

P

K

D

S

H

Q

F

F

D

D

P

L

R

R

Q

K

Y

F

V

F

A

S

D

P

G

A

R

Q

T

L

N

A

I

L

K

K

A

N

I

V

V

V

S

K

H

E

K

R

I

M

K

K

E

-

V

L

L

L

G

G

C

S

D

A

G

N

K

K

binding glyceraldehyde-3-phosphate: S49 (= S50), D82 (= D82), H83 (= H83), H164 (= H180), D239 (= D268), R243 (= R272)
binding zinc ion: H83 (= H83), H83 (= H83), E134 (= E134), H164 (= H180), H194 (= H223), H194 (= H223)

5ucpA Class ii fructose-1,6-bisphosphate aldolase e142a variant of helicobacter pylori with fbp and cleavage products (see paper)
41% identity, 99% coverage: 3:320/321 of query aligns to 2:291/292 of 5ucpA

query
sites
5ucpA

L

L

V

V

T

K

T

G

K

N

E

E

M

I

F

L

K

L

K

K

A

A

Y

H

E

K

G

E

H

G

Y

Y

A

G

I

V

G

G

A

A

F

F

N

N

V

F

N

V

N

N

M

F

E

E

I

M

V

L

Q

N

G

A

I

I

T

F

E

E

A

A

A

G

K

N

E

E

L

E

N

N

A

S

P

P

L

L

I

F

L

I

Q

Q

C

A

S
|
S

A

E

G

G

A

A

R

I

K

K

Y

Y

A

M

K

G

H

I

Q

D

Y

M

L

A

V

V

H

G

L

M

V

V

Q

K

A

I

A

M

I

C

E

E

D

R

T

Y

G

P

-

H

L

I

P

P

I

V

A

A

L

L

H

H

L

L

D
|
D

H
|
H

G

G

A

T

D

T

F

F

E

E

T

S

C

C

K

E

S

K

C

A

I

V

D

K

G

A

G

G

F

F

T

T

S

S

V

V

M

M

F

I

D

D

G

A

S

S

H

H

Y

H

S

A

F

F

K

E

E

E

N

N

I

L

E

E

K

L

T

T

K

S

E

K

V

V

E

K

Y

M

A

A

H

H

A

N

H

A

G

G

V

V

V

S

V

V

E

E

A

A

E
|
E

L

L

G

G

Q

R

L

V

A

-

G

-

I

-

E

-

D

-

V

-

K

-

V

-

A

-

H

-

E

-

A

-

H

-

Y

L

T

V

D

N

P

P

N

K

E

E

V

A

E

E

E

Q

F

F

V

V

K

K

E

E

T

S

G

Q

V

V

D

D

S

Y

L

L

A

A

I

P

A

A

I

I

G

G

T

T

S

S

H
|
H

G

G

A

A

Y

F

K
|
K

F

F

T

K

P

G

D

E

Q

-

C

-

T

-

R

-

N

-

E

-

Q

-

G

-

I

-

L

-

V

-

P

-

P

P

P

K

L

L

R

D

F

F

D

E

I

R

L

L

E

Q

E

E

V

V

A

-

K

K

R

R

L

L

P

T

N

N

F

I

P

P

I

L

V

V

L

L

H
|
H

G
|
G

A

A

S
|
S

S

A

V

I

P

P

Q

D

D

N

F

V

V

R

K

K

I

S

I

Y

N

L

E

D

N

A

G

G

H

D

M

L

P

K

D

G

A

S

V

K

G

G

V

V

P

P

E

F

D

E

Q

F

L

L

R

Q

K

E

A

S

A

V

S

K

M

G

A

G

V

I

C

N

K

K

I

V

N
|
N

I

T

D
|
D

S
x
T

D

D

L

L

R
|
R

L

I

A

A

M

F

T

I

A

A

G

E

I

V

R

R

K

K

H

V

F

A

N

N

D

E

H

D

P

K

D

S

H

Q