SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_008538927.1 NCBI__GCF_000245775.1:WP_008538927.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q53W83 2-dehydro-3-deoxygluconokinase; 2-keto-3-deoxygluconokinase; 3-deoxy-2-oxo-D-gluconate kinase; KDG kinase; EC 2.7.1.45 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
32% identity, 98% coverage: 5:312/313 of query aligns to 4:302/309 of Q53W83

query
sites
Q53W83

L

V

I

V

L

T

V

A

G

G

E

E

P

P

M

L

G

V

L

A

L

L

I

V

A

P

Q

Q

T

E

E

P

G

G

S

H

L

L

N

R

N

G

V

K

S

R

S

L

Y

L

D

E

L

V

A

Y

V

V

A

G

G
|
G

A
|
A

E
|
E

F
x
V

N
|
N

V

V

A

A

I

V

G

A

T

L

A

A

R

R

L

L

E

G

H

V

K

K

V

V

T

G

Y

F

M

V

T

G

K

R

L

V

G

G

D

E

D

D

P

E

F

L

G

G

K

A

R

M

I

V

T

E

V

R

L

L

K

R

D

A

N

E

K

-

I

-

G

G

D

V

E

D

F

L

I

T

S

H

F

F

S

R

K

R

E

A

R

P

A

G

-

F

T

T

G

G

F

L

M

Y

L

L

K

R

G

E

R

Y

T

L

S

P

V

L

G

G

D

Q

P

G

E

R

I

V

F

F

Y
|
Y

F
x
Y

R
|
R

K

K

N

G

S

S

A

A

A

G

S

S

T

A

L

L

N

A

E

P

D

G

D

A

V

F

E

D

K

P

I

D

D

Y

L

L

S

E

N

G

Y

V

G

R

Y

F

I

L

H

H

L

L

T

S

G

G

I

I

L

T

P

P

A

A

L

L

S

S

E

P

D

E

T

A

K

R

K

A

A

F

T

S

F

L

S

W

L

A

I

M

K

E

A

A

K

K

K

R

Q

R

G

G

L

V

F

R

I

V

S

S

F

L

D

D

P

V

N

N

L

Y

R
|
R

P

Q

Q

T

L

L

W

W

K

-

S

S

Q

P

E

E

M

A

I

R

Q

G

T

F

I

L

N

E

E

R

L

A

A

L

S

P

Y

G

A

V

D

D

L

L

V

L

L

F

P

L

G
x
S

Q

E

A

E

E

E

G

A

K

E

I

L

L

L

I

F

G

G

T

-

D

-

E

-

K

-

E

R

I

V

N

E

D

E

F

A

Y

L

L

R

Q

A

N

L

G

S

A

A

S

P

I

E

C

V

V

V

T

L

K
|
K

C
x
R

G
|
G

S
x
A

K
|
K

G
|
G

A
|
A

Y

W

V

A

S

F

T

V

K

-

D

-

G

-

E

D

G

G

Y

R

M

R

V

V

E

E

G

G

-

S

-

A

Y

F

K

A

V

V

E

E

K

-

V

A

V

V

D

D

T

P

V

V

G
|
G

A
|
A

G
|
G

D
|
D

G

A

F

F

A

A

T

A

G

G

V

Y

V

L

T

A

G

G

L

A

M

V

E

W

G

G

L

L

T

P

L

V

S

E

E

E

A

R

V

L

K

R

R

L

G

A

T
x
N

A

L

I

L

G

G

A

A

I

S

Q

V

V

A

M

A

S

S

R

R

G

G

D
|
D

N

H

E

E

G

G

L

A

P

P

T

Y

R

R

Q

E

E

D

L

L

K

E

D

V

F

L

M

L

N

K

GAEVN 34:38 (≠ GAEFN 35:39) binding substrate
YYR 103:105 (≠ YFR 105:107) binding substrate
R167 (= R169) binding substrate
S193 (≠ G196) binding ATP
219:225 (vs. 227:233, 71% identical) binding ATP
GAGD 248:251 (= GAGD 258:261) binding ATP
D251 (= D261) binding substrate
N275 (≠ T285) binding ATP
D287 (= D297) binding substrate

1v1bA 2-keto-3-deoxygluconate kinase from thermus thermophilus with bound atp (see paper)
33% identity, 97% coverage: 5:308/313 of query aligns to 4:298/300 of 1v1bA

query
sites
1v1bA

L

V

I

V

L

T

V

A

G

G

E

E

P

P

M

L

G

V

L

A

L

L

I

V

A

P

Q

Q

T

E

E

P

G

G

S

H

L

L

N

R

N

G

V

K

S

R

S

L

Y

L

D

E

L

V

A

Y

V

V

A

G

G

G

A

A

E

E

F

V

N

N

V

V

A

A

I

V

G

A

T

L

A

A

R

R

L

L

E

G

H

V

K

K

V

V

T

G

Y

F

M

V

T

G

K

R

L

V

G

G

D

E

D

D

P

E

F

L

G

G

K

A

R

M

I

V

T

E

V

R

L

L

K

R

D

A

N

E

K

-

I

-

G

G

D

V

E

D

F

L

I

T

S

H

F

F

S

R

K

R

E

A

R

P

A

G

-

F

T

T

G

G

F

L

M

Y

L

L

K

R

G

E

R

Y

T

L

S

P

V

L

G

G

D

Q

P

G

E

R

I

V

F

F

Y

Y

F

Y

R

R

K

K

N

G

S

S

A

A

A

G

S

S

T

A

L

L

N

A

E

P

D

G

D

A

V

F

E

D

K

P

I

D

D

Y

L

L

S

E

N

G

Y

V

G

R

Y

F

I

L

H

H

L

L

T

S

G

G

I

I

L

T

P

P

A

A

L

L

S

S

E

P

D

E

T

A

K

R

K

A

A

F

T

S

F

L

S

W

L

A

I

M

K

E

A

A

K

K

K

R

Q

R

G

G

L

V

F

R

I

V

S

S

F

L

D

D

P

V

N

N

L

Y

R

R

P

Q

Q

T

L

L

W

W

K

-

S

S

Q

P

E

E

M

A

I

R

Q

G

T

F

I

L

N

E

E

R

L

A

A

L

S

P

Y

G

A

V

D

D

L

L

V

L

L

F

P

L

G

S

Q

E

A

E

E

E

G

A

K

E

I

L

L

L

I

F

G

G

T

-

D

-

E

-

K

-

E

R

I

V

N

E

D

E

F

A

Y

L

L

R

Q

A

N

L

G

S

A

A

S

P

I

E

C

V

V

V

T

L

K
|
K

C

R

G
|
G

S

A

K

K

G

G

A

A

Y

W

V

A

S

F

T

V

K

-

D

-

G

-

E

D

G

G

Y

R

M

R

V

V

E

E

G

G

-

S

-
x
A

Y
x
F

K

A

V
|
V

E

E

K

-

V

A

V

V

D

D

T

P

V

V

G
|
G

A
|
A

G
|
G

D
|
D

G

A

F

F

A

A

T

A

G

G

V

Y

V

L

T

A

G

G

L

A

M

V

E

W

G

G

L

L

T

P

L

V

S

E

E

E

A

R

V

L

K

R

R

L

G

A

T
x
N

A

L

I

L

G

G

A
|
A

I

S

Q

V

V

A

M

A

S

S

R

R

G

G

D

D

N

H

E

E

G

G

L

A

P

P

T

Y

R

R

Q

E

E

D

L

L

K

E

active site: G248 (= G258), A249 (= A259), G250 (= G260), D251 (= D261)
binding adenosine-5'-triphosphate: K219 (= K227), G221 (= G229), A238 (vs. gap), F239 (≠ Y248), V241 (= V250), G248 (= G258), A249 (= A259), G250 (= G260), N275 (≠ T285), A279 (= A289)

1v1aA 2-keto-3-deoxygluconate kinase from thermus thermophilus with bound 2- keto-3-deoxygluconate and adp (see paper)
33% identity, 98% coverage: 5:311/313 of query aligns to 4:301/301 of 1v1aA

query
sites
1v1aA

L

V

I

V

L

T

V

A

G

G

E

E

P

P

M
x
L

G

V

L

A

L

L

I

V

A

P

Q

Q

T

E

E

P

G

G

S

H

L

L

N

R

N

G

V

K

S

R

S

L

Y

L

D

E

L

V

A

Y

V

V

A

G

G
|
G

A
|
A

E

E

F

V

N
|
N

V

V

A

A

I

V

G

A

T

L

A

A

R

R

L

L

E

G

H

V

K

K

V

V

T

G

Y

F

M

V

T

G

K

R

L

V

G

G

D

E

D

D

P

E

F

L

G

G

K

A

R

M

I

V

T

E

V

R

L

L

K

R

D

A

N

E

K

-

I

-

G

G

D

V

E

D

F

L

I

T

S

H

F

F

S

R

K

R

E

A

R

P

A

G

-

F

T

T

G

G

F

L

M
x
Y

L

L

K

R

G

E

R

Y

T

L

S

P

V

L

G

G

D

Q

P

G

E

R

I

V

F

F

Y

Y

F

Y

R
|
R

K

K

N

G

S

S

A

A

A

G

S

S

T

A

L

L

N

A

E

P

D

G

D

A

V

F

E

D

K

P

I

D

D

Y

L

L

S

E

N

G

Y

V

G

R

Y

F

I

L

H

H

L

L

T

S

G

G

I

I

L

T

P

P

A

A

L

L

S

S

E

P

D

E

T

A

K

R

K

A

A

F

T

S

F

L

S

W

L

A

I

M

K

E

A

A

K

K

K

R

Q

R

G

G

L

V

F

R

I

V

S

S

F

L

D

D

P

V

N

N

L

Y

R
|
R

P

Q

Q

T

L

L

W

W

K

-

S

S

Q

P

E

E

M

A

I

R

Q

G

T

F

I

L

N

E

E

R

L

A

A

L

S

P

Y

G

A

V

D

D

L

L

V

L

L

F

P

L

G

S

Q

E

A

E

E

E

G

A

K

E

I

L

L

L

I

F

G

G

T

R

D

V

D

E

E

E

K

A

E

-

I

-

N

-

D

-

F

-

Y

L

L

R

Q

A

N

L

G

S

A

A

S

P

I

E

C

V

V

V

T

L

K
|
K

C

R

G
|
G

S
x
A

K

K

G

G

A

A

Y

W

V

A

S

F

T

V

K

-

D

-

G

-

E

D

G

G

Y

R

M

R

V

V

E

E

G

G

-

S

-

A

Y

F

K

A

V

V

E

E

K

-

V

A

V

V

D

D

T

P

V

V

G
|
G

A
|
A

G
|
G

D
|
D

G

A

F

F

A

A

T

A

G

G

V

Y

V

L

T

A

G

G

L

A

M

V

E

W

G

G

L

L

T

P

L

V

S

E

E

E

A

R

V

L

K

R

R

L

G

A

T
x
N

A

L

I

L

G

G

A
|
A

I

S

Q

V

V

A

M

A

S

S

R

R

G

G

D
|
D

N

H

E

E

G

G

L

A

P

P

T

Y

R

R

Q

E

E

D

L

L

K

E

D

V

F

L

M

L

active site: G248 (= G258), A249 (= A259), G250 (= G260), D251 (= D261)
binding adenosine-5'-diphosphate: K219 (= K227), G221 (= G229), A222 (≠ S230), A249 (= A259), G250 (= G260), N275 (≠ T285), A279 (= A289)
binding 2-keto-3-deoxygluconate: L11 (≠ M12), G34 (= G35), A35 (= A36), N38 (= N39), Y89 (≠ M91), R105 (= R107), R167 (= R169), G248 (= G258), D251 (= D261), D287 (= D297)

2varA Crystal structure of sulfolobus solfataricus 2-keto-3-deoxygluconate kinase complexed with 2-keto-3-deoxygluconate (see paper)
31% identity, 99% coverage: 5:313/313 of query aligns to 3:309/311 of 2varA

query
sites
2varA

L

V

I

I

L

A

V

L

G

G

E

E

P

P

M
x
L

G

I

L

Q

L

F

I

N

A

S

Q

F

T

N

E

P

G

G

S

P

L

L

N

R

N

F

V

V

S

N

S

Y

Y

F

D

E

L

K

A

H

V

V

A

A

G
|
G

A
x
S

E

E

F

L

N

N

V

F

A

C

I

I

G

A

T

V

A

V

R

R

L

N

E

H

H

L

K

S

V

C

T

S

Y

L

M

I

T

A

K

R

L

V

G

G

D

N

D

D

P

E

F

F

G

G

K

K

R

N

I

I

T

I

T

E

V

Y

L

S

K

R

D

A

N

Q

K

G

I

I

G

D

D

T

E

S

F

H

I

I

S

K

F

V

S

D

K

N

E

E

R

S

A

F

T

T

G

G

-

I

-
x
Y

F

F

M

I

L

Q

K

R

G

G

R

Y

T

P

S

I

V

P

G

M

D

K

P

S

E

E

I
x
L

F

V

Y
|
Y

F

Y

R
|
R

K

K

N

G

S

S

A

A

A

G

S

S

T

R

L

L

N

S

E

P

D

E

D

D

V

I

E

N

K

E

I

N

D

Y

L

V

S

R

N

N

Y

S

G

R

Y

L

I

V

H

H

L

S

T

T

G

G

I
|
I

L

T

P

L

A

A

L

I

S

S

E

D

D

N

T

A

K

K

K

E

A

A

T

V

F

I

S

K

L

A

I

F

K

E

K

L

A

A

K

K

K

S

Q

R

G

-

L

-

F

-

I

-

S

S

F

L

D

D

P

T

N

N

L

I

R
|
R

P

P

Q

K

L

L

W

W

K

S

S

S

Q

L

E

E

E

K

M

A

I

K

Q

E

T

T

I

I

N

L

E

S

L

I

A

L

S

K

Y

K

A

Y

D

D

L

I

-

E

-

V

V

L

L

I

P

T

G

D

Q

P

A

D

E

D

G

T

K

K

I

I

L

L

I

L

G

D

T

V

D

T

D

D

E

P

K

D

E

E

I

A

N

Y

D

R

F

K

Y

Y

L

K

Q

E

N

L

G

G

A

V

S

K

I

V

C

L

V

L

T

Y

K
|
K

C

L

G
|
G

S
|
S

K

K

G
|
G

A

A

Y

-

V

I

S

A

T

Y

K

K

D

D

G

N

E

V

G

K

Y

A

M

F

V

K

E

D

G

A

Y

Y

K

K

V

V

E

-

K

P

V

V

V

E

D

D

T

P

V
x
T

G
|
G

A
|
A

G
|
G

D
|
D

G

A

F
x
M

A

A

T

G

G

T

V

F

V

V

T

S

G

L

L

Y

M

L

E

Q

G

G

L

K

T

D

L

I

S

E

E

Y

A

S

V

L

K

A

R

H

G

G

T
x
I

A

A

I

A

G
x
S

A

T

I

L

Q

V

V
x
I

M

T

S

V

R

R

G

G

D
|
D

N

N

E

E

G

L

L

T

P

P

T

T

R

L

Q

E

E

D

L

A

K

E

D

R

F

F

M

L

N

N

K

E

active site: G254 (= G258), A255 (= A259), G256 (= G260), D257 (= D261)
binding adenosine monophosphate: G227 (= G229), G230 (= G232), M259 (≠ F263), S284 (≠ G288), I288 (≠ V292)
binding phosphoaminophosphonic acid-adenylate ester: K225 (= K227), G227 (= G229), S228 (= S230), G230 (= G232), G254 (= G258), A255 (= A259), G256 (= G260), D257 (= D261), M259 (≠ F263), I281 (≠ T285), S284 (≠ G288), I288 (≠ V292)
binding 3-deoxy-alpha-D-erythro-hex-2-ulofuranosonic acid: L10 (≠ M12), G33 (= G35), S34 (≠ A36), Y89 (vs. gap), Y105 (= Y105), R107 (= R107), I136 (= I136), R165 (= R169), G254 (= G258), D257 (= D261)
binding 2-keto-3-deoxygluconate: G33 (= G35), S34 (≠ A36), Y89 (vs. gap), L103 (≠ I103), Y105 (= Y105), R107 (= R107), I136 (= I136), R165 (= R169), T253 (≠ V257), G254 (= G258), D257 (= D261), D293 (= D297)

Q97U29 2-dehydro-3-deoxygluconokinase/2-dehydro-3-deoxygalactonokinase; 2-dehydro-3-deoxyglucono/galactono-kinase; 2-keto-3-deoxy-galactonokinase; 2-keto-3-deoxygluconokinase; 3-deoxy-2-oxo-D-gluconate kinase; KDG kinase; KDGal kinase; EC 2.7.1.178 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
31% identity, 99% coverage: 5:313/313 of query aligns to 4:310/313 of Q97U29

query
sites
Q97U29

L

V

I

I

L

A

V

L

G

G

E

E

P

P

M

L

G

I

L

Q

L

F

I

N

A

S

Q

F

T

N

E

P

G

G

S

P

L

L

N

R

N

F

V

V

S

N

S

Y

Y

F

D

E

L

K

A

H

V

V

A

A

G
|
G

A
x
S

E
|
E

F
x
L

N
|
N

V

F

A

C

I

I

G

A

T

V

A

V

R

R

L

N

E

H

H

L

K

S

V

C

T

S

Y

L

M

I

T

A

K

R

L

V

G

G

D

N

D

D

P

E

F

F

G

G

K

K

R

N

I

I

T

I

T

E

V

Y

L

S

K

R

D

A

N

Q

K

G

I

I

G

D

D

T

E

S

F

H

I

I

S

K

F

V

S

D

K

N

E

E

R

S

A

F

T

T

G

G

-

I

-
x
Y

F

F

M

I

L

Q

K

R

G

G

R

Y

T

P

S

I

V

P

G

M

D

K

P

S

E

E

I

L

F

V

Y
|
Y

F
x
Y

R
|
R

K

K

N

G

S

S

A

A

A

G

S

S

T

R

L

L

N

S

E

P

D

E

D

D

V

I

E

N

K

E

I

N

D

Y

L

V

S

R

N

N

Y

S

G

R

Y

L

I

V

H

H

L

S

T

T

G

G

I

I

L

T

P

L

A

A

L

I

S

S

E

D

D

N

T

A

K

K

K

E

A

A

T

V

F

I

S

K

L

A

I

F

K

E

K

L

A

A

K

K

K

S

Q

R

G

-

L

-

F

-

I

-

S

S

F

L

D

D

P

T

N
|
N

L
x
I

R
|
R

P

P

Q

K

L

L

W

W

K

S

S

S

Q

L

E

E

E

K

M

A

I

K

Q

E

T

T

I

I

N

L

E

S

L

I

A

L

S

K

Y

K

A

Y

D

D

L

I

-

E

-

V

V

L

L

I

P

T

G

D

Q

P

A

D

E

D

G

T

K

K

I

I

L

L

I

L

G

D

T

V

D

T

D

D

E

P

K

D

E

E

I

A

N

Y

D

R

F

K

Y

Y

L

K

Q

E

N

L

G

G

A

V

S

K

I

V

C

L

V

L

T

Y

K
|
K

C
x
L

G
|
G

S
|
S

K
|
K

G
|
G

A

A

Y

-

V

I

S

A

T

Y

K

K

D

D

G

N

E

V

G

K

Y

A

M

F

V

K

E

D

G

A

Y

Y

K

K

V

V

E

-

K

P

V

V

V

E

D

D

T

P

V

T

G
|
G

A
|
A

G
|
G

D
|
D

G

A

F

M

A

A

T

G

G

T

V

F

V

V

T

S

G

L

L

Y

M

L

E

Q

G

G

L

K

T

D

L

I

S

E

E

Y

A

S

V

L

K

A

R

H

G

G

T

I

A

A

I

A

G

S

A

T

I

L

Q

V

V

I

M

T

S

V

R

R

G

G

D
|
D

N

N

E

E

G

L

L

T

P

P

T

T

R

L

Q

E

E

D

L

A

K

E

D

R

F

F

M

L

N

N

K

E

GSELN 34:38 (≠ GAEFN 35:39) binding substrate
Y90 (vs. gap) binding substrate
YYR 106:108 (≠ YFR 105:107) binding substrate
NIR 164:166 (≠ NLR 167:169) binding ATP
R166 (= R169) binding substrate
KLGSKG 226:231 (≠ KCGSKG 227:232) binding ATP
GAGD 255:258 (= GAGD 258:261) binding ATP
D258 (= D261) binding substrate
D294 (= D297) binding substrate

2dcnA Crystal structure of 2-keto-3-deoxygluconate kinase from sulfolobus tokodaii complexed with 2-keto-6-phosphogluconate (alpha-furanose form)
29% identity, 99% coverage: 5:313/313 of query aligns to 3:307/308 of 2dcnA

query
sites
2dcnA

L

L

I

I

L

T

V

L

G

G

E

E

P

I

M

L

G

I

L

E

L

F

I

N

A

A

Q

L

T

S

E

P

G

G

S

P

L

L

N

R

N

H

V

V

S

S

S

Y

Y

F

D

E

L

K

A

H

V

V

A

A

G
|
G

A

S

E

E

F

A

N

N

V

Y

A

C

I

V

G

A

T

F

A

I

R

K

L

Q

E

G

H

N

K

E

V

C

T

G

Y

I

M

I

T

A

K

K

L

V

G

G

D

D

P

E

F

F

G

G

K

Y

R

N

I

A

T

I

T

E

V

W

L

L

K

R

D

G

N

Q

K

G

I

V

G

D

D

V

E

S

F

H

I

M

S

K

F

I

S

D

K

P

E

S

R

A

A

P

T

T

G

G

-

I

F
|
F

M

F

L

I

K

Q

G

R

R

H

T

Y

S

P

V

V

G

P

-

L

D

K

P

S

E

E

I

S

F

I

Y
|
Y

F

Y

R
|
R

K

K

N

G

S

S

A

A

A

G

S

S

T

K

L

L

N

S

E

P

D

E

D

D

V

V

E

D

K

E

I

E

D

Y

L

V

S

K

N

S

Y

A

G

D

Y

L

I

V

H

H

L

S

T

S

G

G

I
|
I

L

T

P

L

A

A

L

I

S

S

E

S

D

T

T

A

K

K

K

E

A

A

T

V

F

Y

S

K

L

A

I

F

K

E

K

I

A

A

K

S

K

N

Q

R

G

-

L

-

F

-

I

-

S

S

F

F

D

D

P

T

N

N

L

I

R
|
R

P

L

Q

K

L

L

W

W

K

S

S

A

Q

E

E

E

E

A

M

K

I

R

Q

E

T

I

I

L

N

K

E

L

L

L

A

S

S

K

Y

F

A

H

-

L

D

K

L

F

V

L

L

I

P

T

G
x
D

Q

T

A

D

E

D

G

S

K

K

I

I

L

I

I

L

G

G

-

E

T

S

D

D

D

P

E

D

K

K

E

A

I

A

N

K

D

A

F

F

Y

-

L

-

Q

S

N

D

G

Y

A

A

S

E

I

I

C

I

V

V

T

M

K
|
K

C

L

G
|
G

S
x
P

K

K

G
|
G

A

A

Y

I

V

V

S

Y

T

Y

K

-

D

D

G

G

E

K

G

K

Y

Y

M

Y

V

S

E

S

G

G

Y

Y

K

Q

V

V

E

-

K

P

V
|
V

V

E

D

D

T

V

V
x
T

G
|
G

A
|
A

G
|
G

D
|
D

G

A

F

L

A

G

T

G

G

T

V

F

V

L

T

S

G

L

L

Y

M

Y

E

K

G

G

L

F

T

E

L

M

S

E

E

K

A

A

V

L

K

D

R

Y

G

A

T
x
I

A

V

I

A

G
x
S

A

T

I

L

Q

N

V
|
V

M

M

S

I

R

R

G

G

D
|
D

N

Q

E

E

G

N

L

L

P

P

T

T

R

T

Q

K

E

D

L

I

K

E

D

T

F

F

M

L

N

R

K

E

active site: G252 (= G258), A253 (= A259), G254 (= G260), D255 (= D261)
binding adenosine-5'-diphosphate: D193 (≠ G196), K223 (= K227), G225 (= G229), P226 (≠ S230), G228 (= G232), V247 (= V253), G254 (= G260), I279 (≠ T285), S282 (≠ G288), V286 (= V292)
binding 6-O-phosphono-beta-D-psicofuranosonic acid: G33 (= G35), F89 (= F90), Y105 (= Y105), R107 (= R107), I136 (= I136), R165 (= R169), T251 (≠ V257), G252 (= G258), D255 (= D261), D291 (= D297)

5eynA Crystal structure of fructokinase from vibrio cholerae o395 in fructose, adp, beryllium trifluoride and calcium ion bound form
29% identity, 88% coverage: 35:311/313 of query aligns to 28:305/306 of 5eynA

query
sites
5eynA

G
|
G

A
|
A

E

P

F

A

N
|
N

V

V

A

A

I

V

G

A

T

I

A

A

R

R

L

L

E

S

H

G

K

R

V

S

T

A

Y

F

M

F

T

G

K

R

L

V

G

G

D

N

D

D

P

P

F

F

G

G

K

R

R

F

I

M

T

Q

V

T

L

L

K

T

D

D

N

E

K

Q

I

V

G

D

D

C

E

Q

F

H

I

L

S

H

F

F

S

D

K

P

E

V

R

H

A

R

T

T

G

S

F

T

M

V

L

V

K

V

G

D

R

L

T

D

S

E

V
x
H

G

G

D

E

P

R

E

S

I
x
F

F

T

Y
x
F

F

M

R

V

K

K

N

P

S

S

A

A

A

D

S

Q

T

F

L

L

N

Q

E

L

D

S

D

D

V

I

E

P

K

S

I

F

D

Q

L

K

S

G

N

E

Y

W

G

-

Y

-

I

L

H

H

L

V

T

C

G

S

I

I

L

A

P

L

A

A

L

-

S

N

E

Q

D

P

T

S

K

R

K

S

A

S

T

T

F

F

S

A

L

A

I

I

K

A

K

Q

A

M

K

K

K

E

Q

V

G

G

L

G

F

Y

I

V

S

S

F

F

D

D

P

P

N

N

L

L

R
|
R

P

E

Q

E

L

V

W

W

K

S

S

E

Q

P

E

Q

E

E

M

L

I

Q

Q

A

T

T

I

V

N

M

E

R

L

A

A

V

S

G

Y

L

A

A

D

D

L

V

V

V

L

K

P

F

G

S

Q

E

A

E

E

E

G

L

K

Q

I

F

L

L

I

T

G

G

T

T

D

Q

D

S

E

I

K

E

E

E

-

G

-

L

-

Q

-

A

I

I

N

A

D

D

F

F

Y

Q

L

I

Q

-

N

-

G

-

A

-

S

P

I

L

C

V

V

V

T

V

K
x
T

C

L

G
|
G

S
x
A

K

K

G

G

A

A

Y

L

V

V

S

A

T

T

K

P

D

N

G

S

E

Q

G

-

Y

Q

M

I

V

V

E

S

G

G

Y

-

K

K

V
x
A

E
x
V

K

K

V

P

V

I

D

D

T
|
T

V

T

G
|
G

A
|
A

G
|
G

D
|
D

G

A

F
|
F

A

V

T

G

G

G

V

L

T

Y

G

R

L

L

-

S

-

V

-

A

-

Q

-

D

-

W

M

H

E

N

G

Q

L

A

T

T

L

I

S

L

E

D

A

A

V

V

K

K

R

W

G

A

T
x
N

A

G

I

C

G
|
G

A
|
A

I

L

Q

A

V

T

M

T

S

Q

R

K

G

G

D

A

N

M

E

T

G

A

L

L

P

P

T

N

R

Q

Q

A

E

A

L

L

K

Y

D

A

F

F

M

L

active site: G246 (= G258), A247 (= A259), G248 (= G260), D249 (= D261)
binding adenosine-5'-diphosphate: H91 (≠ V98), T217 (≠ K227), G219 (= G229), A220 (≠ S230), A238 (≠ V250), V239 (≠ E251), T244 (= T256), G246 (= G258), A247 (= A259), G248 (= G260), F251 (= F263), N279 (≠ T285), G282 (= G288), A283 (= A289)
binding beryllium trifluoride ion: G246 (= G258), G248 (= G260), D249 (= D261)
binding beta-D-fructofuranose: G28 (= G35), A29 (= A36), N32 (= N39), F96 (≠ I103), F98 (≠ Y105), R159 (= R169), D249 (= D261)

Sites not aligning to the query:

binding beta-D-fructofuranose: 9, 13

5yggA Crystal structure of fructokinase double-mutant (t261c-h108c) from vibrio cholerae o395 in fructose, adp and potassium ion bound form (see paper)
29% identity, 88% coverage: 35:311/313 of query aligns to 32:309/310 of 5yggA

query
sites
5yggA

G
|
G

A
|
A

E

P

F

A

N

N

V

V

A

A

I

V

G

A

T

I

A

A

R

R

L

L

E

S

H

G

K

R

V

S

T

A

Y

F

M

F

T

G

K

R

L

V

G

G

D

N

D

D

P

P

F

F

G

G

K

R

R

F

I

M

T

Q

V

T

L

L

K

T

D

D

N

E

K

Q

I

V

G

D

D

C

E

Q

F

H

I

L

S

H

F

F

S

D

K

P

E

V

R

H

A

R

T

T

G

S

F

T

M

V

L

V

K

V

G

D

R

L

T

D

S

E

V

C

G

G

D

E

P

R

E

S

I
x
F

F

T

Y
x
F

F

M

R

V

K

K

N

P

S

S

A

A

A

D

S

Q

T

F

L

L

N

Q

E

L

D

S

D

D

V

I

E

P

K

S

I

F

D

Q

L

K

S

G

N

E

Y

W

G

-

Y

-

I

L

H

H

L

V

T

C

G

S

I

I

L

A

P

L

A

A

L

-

S

N

E

Q

D

P

T

S

K

R

K

S

A

S

T

T

F

F

S

A

L

A

I

I

K

A

K

Q

A

M

K

K

K

E

Q

V

G

G

L

G

F

Y

I

V

S

S

F

F

D

D

P

P

N

N

L

L

R
|
R

P

E

Q

E

L

V

W

W

K

S

S

E

Q

P

E

Q

E

E

M

L

I

Q

Q

A

T

T

I

V

N

M

E

R

L

A

A

V

S

G

Y

L

A

A

D

D

L

V

V

V

L
x
K

P

F

G

S

Q

E

A

E

E

E

G

L

K

Q

I

F

L

L

I

T

G

G

T

T

D

Q

D

S

E

I

K

E

E

E

-

G

-

L

-

Q

-

A

I

I

N

A

D

D

F

F

Y

Q

L

I

Q

-

N

-

G

-

A

-

S

P

I

L

C

V

V

V

T

V

K
x
T

C

L

G
|
G

S

A

K

K

G

G

A

A

Y

L

V

V

S

A

T

T

K

P

D

N

G

S

E

Q

G

-

Y

Q

M

I

V

V

E

S

G

G

Y

-

K

K

V
x
A

E
x
V

K

K

V

P

V

I

D

D

T

C

V

T

G

G

A

A

G

G

D
|
D

G

A

F
|
F

A

V

T

G

G

G

V

L

T

Y

G

R

L

L

-

S

-

V

-

A

-

Q

-

D

-

W

M

H

E

N

G

Q

L

A

T

T

L

I

S

L

E

D

A

A

V

V

K

K

R

W

G

A

T
x
N

A

G

I

C

G
|
G

A
|
A

I

L

Q

A

V

T

M

T

S

Q

R

K

G

G

D

A

N

M

E

T

G

A

L

L

P

P

T

N

R

Q

Q

A

E

A

L

L

K

Y

D

A

F

F

M

L

binding adenosine-5'-diphosphate: K188 (≠ L194), T221 (≠ K227), G223 (= G229), A242 (≠ V250), V243 (≠ E251), F255 (= F263), N283 (≠ T285), G286 (= G288), A287 (= A289)
binding beta-D-fructofuranose: G32 (= G35), A33 (= A36), F100 (≠ I103), F102 (≠ Y105), R163 (= R169), D253 (= D261)

Sites not aligning to the query:

binding beta-D-fructofuranose: 13, 17

3in1A Crystal structure of a putative ribokinase in complex with adp from e.Coli
29% identity, 92% coverage: 25:311/313 of query aligns to 25:308/312 of 3in1A

query
sites
3in1A

N

D

V

V

S

D

S

S

Y

Y

D

P

L

L

-

E

-

R

-

I

-

A

-

M

A

T

V

T

A

G

G

G

A

D

E

A

F

I

N

N

V

E

A

A

I

T

G

I

T

I

A

S

R

R

L

L

E

G

H

H

K

R

V

T

T

A

Y

L

M

M

T

S

K

R

L

I

G

G

D

K

D

D

P

A

F

A

G

G

K

Q

R

F

I

I

T

L

T

D

V

H

L

C

K

R

D

K

N

E

K

N

I

I

G

D

D

I

E

Q

F

S

I

L

S

K

F

Q

S

D

K

V

E

S

R

I

A

D

T

T

G

S

F

I

M

N

L

V

K

G

G

L

R

V

T

T

S

E

V

D

G

G

D

E

P

-

E
x
R

I

T

F

F

Y

V

F

T

R

N

K

R

N

N

S

G

A

S

A

L

S

W

T

K

L

L

N

N

E

I

D

D

V

V

E

D

K

F

I

A

D

R

L

F

S

S

N

Q

Y

A

G

K

Y

L

I

L

H

S

L

L

T

A

G

S

I

I

L

F

P

N

A

S

L

P

S

L

E

L

D

D

T

G

K

K

K

A

A

L

T

T

F

-

S

E

L

I

I

F

K

T

K

Q

A

A

K

K

K

A

Q

R

G

Q

L

M

F

I

I

I

S

C

F

A

D

D

P

M

N

-

L

I

R

K

P

P

Q

R

L

L

W

-

K

-

S

-

Q

-

E

N

E

E

M

T

I

L

Q

D

T

D

I

I

N

C

E

E

L

A

A

L

S

S

Y

Y

A

V

D

D

L

Y

V

L

L

F

P

P

G
x
N

Q

F

A

A

E

E

G

A

K

K

I

L

L

L

I

T

G

G

T

K

D

E

D

T

E

L

K

D

E

E

I

I

N

A

D

D

F

C

Y

F

L

L

Q

A

N

C

G

G

A

V

S

K

I

T

C

V

V

V

T

I

K
|
K

C

T

G
|
G

S

K

K

D

G
|
G

A

C

Y

F

V

I

S

-

T

-

K

K

D

R

G

G

E

D

G

M

Y

T

M

M

-

K

V

V

E

P

G
x
A

Y

V

K

A

V

G

E

I

K

T

V

A

V

I

D

D

T
|
T

V

I

G
|
G

A
|
A

G
|
G

D
|
D

G

N

F

F

A

A

T

S

G

G

V

F

V

I

T

A

G

A

L

L

M

L

E

E

G

G

L

K

T

N

L

L

S

R

E

E

A

C

V

A

K

R

R

F

G

A

T
x
N

A

A

I

T

G
x
A

A

A

I

I

Q

S

V

V

M

L

S

S

R

V

G

G

D

A

N

T

E

T

G

G

L

V

P

K

T

N

R

R

Q

K

E

L

L

V

K

E

D

Q

F

L

M

L

active site: R106 (≠ E102), G255 (= G258), A256 (= A259), G257 (= G260), D258 (= D261)
binding adenosine-5'-diphosphate: N194 (≠ G196), K225 (= K227), G227 (= G229), G230 (= G232), A244 (≠ G247), T253 (= T256), N282 (≠ T285), A285 (≠ G288)

Q8ZKR2 Aminoimidazole riboside kinase; AIRs kinase; EC 2.7.1.223 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
25% identity, 92% coverage: 27:313/313 of query aligns to 23:309/319 of Q8ZKR2

query
sites
Q8ZKR2

S

N

S

S

Y

Y

D

L

L

K

A

C

V

P

A

G

G
|
G

A

A

E

S

F

A

N

N

V

V

A

G

I

V

G

C

T

V

A

A

R

R

L

L

E

G

H

G

K

E

V

C

T

G

Y

F

M

I

T

G

K

C

L

L

G

G

D

D

P

D

F

A

G

G

K

R

R

F

I

L

T

R

T

Q

V

V

L

F

K

Q

D

D

N

N

K

G

I

V

G

D

D

V

E

T

F

F

I

L

S

R

F

L

S

D

K

A

E

D

R

L

A

T

T

S

G

A

F

V

M

L

L

I

K

V

G

N

R

L

T

T

S

A

V

D

G

G

D

E

P

R

E

S

I

F

F

T

Y
|
Y

F

L

R

V

K

H

N

P

S

G

A

A

A

D

S

T

T

Y

L

V

N

S

E

P

D

Q

D

D

V

L

E

P

K

P

I

F

D

R

L

Q

S

Y

N

E

Y

W

G

F

Y

Y

I

F

H

S

L

S

T

I

G

G

I

L

L

-

P

-

A

-

L

T

S

D

E

R

D

P

T

A

K

R

K

E

A

A

T

C

F

L

S

E

L

G

I

A

K

R

A

M

K

R

K

E

Q

A

G

G

L

G

F

Y

I

V

S

L

F

F

D

D

P

V

N

N

L

L

R
|
R

P

S

Q

K

L

M

W

W

K

G

S

N

Q

T

E

D

E

E

M

I

I

P

Q

E

T

L

I

I

N

A

E

R

L

S

A
|
A

S
x
A

Y

L

A
|
A

D

S

L

I

V

C

L

K

P

V

G

S

Q

A

A

D

E

E

G

L

K

C

I

Q

L

L

I

S

G

G

T

A

D

S

D

H

E

W

K

Q

E

D

I

A

N

R

D

-

F

Y

Y

Y

L

L

Q

R

N

D

-

L

G
|
G

A

C

S

D

I

T

C

T

V

I

T

I

K

S

C

L

G

G

S

A

K

D

G

G

A

A

Y

L

V

L

S

I

T

T

K

A

D

E

G

G

E

E

G

-

Y

F

M

H

V

F

E

P

G

A

Y

P

K

R

V

V

E

D

K

-

V

V

D
|
D

T

T

V
x
T

G

G

A

A

G

G

D
|
D

G

A

F

F

A

V

T

G

G

G

V

L

T

F

G

T

L

L

-

S

-

R

-

A

-

N

-

C

M

W

E

D

G

H

L

A

T

L

L

L

S

A

E

E

A

A

V

I

K

S

R

N

G

A

T

N

A

A

I

C

G

G

A

A

I

M

Q
x
A

V

V

M

T

S
x
A

R

K

G
|
G

D

A

N

M

E

T

G

A

L

L

P

P

T

F

R

P

Q

D

E

Q

L

L

K

N

D

T

F

F

M

L

N

S

K

S

G31 (= G35) binding 5-amino-1-(beta-D-ribosyl)imidazole
Y101 (= Y105) binding 5-amino-1-(beta-D-ribosyl)imidazole
R162 (= R169) binding 5-amino-1-(beta-D-ribosyl)imidazole
A180 (= A187) binding K(+)
A181 (≠ S188) binding K(+)
A183 (= A190) binding K(+)
G213 (= G220) binding K(+)
D246 (= D255) binding K(+)
T248 (≠ V257) binding K(+)
D252 (= D261) binding 5-amino-1-(beta-D-ribosyl)imidazole
A287 (≠ Q291) binding K(+)
A290 (≠ S294) binding K(+)
G292 (= G296) binding K(+)

Sites not aligning to the query:

16 binding 5-amino-1-(beta-D-ribosyl)imidazole

3gbuA Crystal structure of an uncharacterized sugar kinase ph1459 from pyrococcus horikoshii in complex with atp
30% identity, 84% coverage: 8:271/313 of query aligns to 5:255/302 of 3gbuA

query
sites
3gbuA

V

I

G

G

E

E

P

L

M

L

G

I

L

D

L

L

I

I

A

S

Q

V

T

E

E

E

G

G

S

D

L

L

N

K

N

D

V

V

S

R

S

L

Y

F

D

E

L

K

A

H

V

P

A

G

G

G

A

A

E

P

F

A

N

N

V

V

A

A

I

V

G

G

T

V

A

S

R

R

L

L

E

G

H

V

K

K

V

S

T

S

Y

L

M

I

T

S

K

K

L

V

G

G

D

N

D

D

P

P

F

F

G

G

K

E

R

Y

I

L

T

I

T

E

V

E

L

L

K

S

D

K

N

E

K

N

I

V

G

D

D

T

E

R

F

G

I

I

S

V

F

K

S

D

K

E

E

K

R

K

A

H

T

T

G

G

F

I

M

V

-

F

-

V

-

Q

L

L

K

K

G

G

R

A

T

S

S

P

-

S

-

F

V

L

G

L

D

Y

P

D

E

D

I

V

F

A

Y

Y

F

F

R

-

K

-

N

N

S

M

A

T

A

L

S

N

T

D

L

I

N

N

E

W

D

D

D

I

V

V

E

E

K

E

I

A

D

K

L

I

S

V

N

N

Y

F

G

G

Y

S

I

V

H

I

L

L

T

A

G

-

I

-

L

-

P

-

A

-

L

-

S

R

E

N

D

P

T

S

K

R

K

E

A

T

T

V

F

M

S

K

L

V

I

I

K

K

A

I

K

K

K

G

Q

S

G

S

L

L

F

-

I

I

S

A

F

F

D

D

P

V

N

N

L

L

R

R

P

L

Q

D

L

L

W

W

K

R

S

G

Q

Q

-

E

E

E

M

M

I

I

Q

K

T

V

I

L

N

E

E

E

L

S

A

I

S

K

Y

L

A

A

D

D

L

I

V

V

L
x
K

P

A

G

S

Q

E

A

E

E

E

G

V

K

-

I

-

L

-

I

-

G

-

T

-

D

-

E

-

K

-

E

-

I

-

N

-

D

-

F

L

Y

Y

L

L

Q

E

N

N

-

Q

-

G

-

V

-

E

-

V

-

K

G

G

A

S

S

M

I

L

C

T

V

A

T

I

K
x
T

C

L

G
|
G

S

P

K

K

G

G

A

-

Y

F

V

R

S

L

T

I

K

K

D

N

G

E

E

T

G

V

Y

V

M

D

V

V

E

P

G

S

Y

Y

K

N

V
|
V

E

N

K
x
P

V

-

V

L

D

D

T

T

V

T

G
|
G

A
|
A

G
|
G

D
|
D

G

A

F

F

A

M

T

A

G

A

V

L

T

V

G

G

L

I

active site: G242 (= G258), A243 (= A259), G244 (= G260), D245 (= D261)
binding adenosine-5'-triphosphate: K188 (≠ L194), T213 (≠ K227), G215 (= G229), V235 (= V250), P237 (≠ K252), A243 (= A259), G244 (= G260)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 274

3ih0A Crystal structure of an uncharacterized sugar kinase ph1459 from pyrococcus horikoshii in complex with amp-pnp
30% identity, 84% coverage: 8:271/313 of query aligns to 6:256/304 of 3ih0A

query
sites
3ih0A

V

I

G

G

E

E

P

L

M

L

G

I

L

D

L

L

I

I

A

S

Q

V

T

E

E

E

G

G

S

D

L

L

N

K

N

D

V

V

S

R

S

L

Y

F

D

E

L

K

A

H

V

P

A

G

G

G

A

A

E

P

F

A

N

N

V

V

A

A

I

V

G

G

T

V

A

S

R

R

L

L

E

G

H

V

K

K

V

S

T

S

Y

L

M

I

T

S

K

K

L

V

G

G

D

N

D

D

P

P

F

F

G

G

K

E

R

Y

I

L

T

I

T

E

V

E

L

L

K

S

D

K

N

E

K

N

I

V

G

D

D

T

E

R

F

G

I

I

S

V

F

K

S

D

K

E

E

K

R

K

A

H

T

T

G

G

F

I

M

V

-

F

-

V

-

Q

L

L

K

K

G

G

R

A

T

S

S

P

-

S

-

F

V

L

G

L

D

Y

P

D

E

D

I

V

F

A

Y

Y

F

F

R

-

K

-

N

N

S

M

A

T

A

L

S

N

T

D

L

I

N

N

E

W

D

D

D

I

V

V

E

E

K

E

I

A

D

K

L

I

S

V

N

N

Y

F

G

G

Y

S

I

V

H

I

L

L

T

A

G

-

I

-

L

-

P

-

A

-

L

-

S

R

E

N

D

P

T

S

K

R

K

E

A

T

T

V

F

M

S

K

L

V

I

I

K

K

A

I

K

K

K

G

Q

S

G

S

L

L

F

-

I

I

S

A

F

F

D

D

P

V

N

N

L

L

R

R

P

L

Q

D

L

L

W

W

K

R

S

G

Q

Q

-

E

E

E

M

M

I

I

Q

K

T

V

I

L

N

E

E

E

L

S

A

I

S

K

Y

L

A

A

D

D

L

I

V

V

L

K

P

A

G

S

Q

E

A

E

E

E

G

V

K

-

I

-

L

-

I

-

G

-

T

-

D

-

E

-

K

-

E

-

I

-

N

-

D

-

F

L

Y

Y

L

L

Q

E

N

N

-

Q

-

G

-

V

-

E

-

V

-

K

G

G

A

S

S

M

I

L

C

T

V

A

T

I

K
x
T

C

L

G
|
G

S

P

K

K

G

G

A

-

Y

F

V

R

S

L

T

I

K

K

D

N

G

E

E

T

G

V

Y

V

M

D

V

V

E

P

G

S

Y

Y

K

N

V
|
V

E

N

K

P

V

-

V

L

D

D

T

T

V

T

G
|
G

A
|
A

G
|
G

D
|
D

G

A

F

F

A

M

T

A

G

A

V

L

T

V

G

G

L

I

active site: G243 (= G258), A244 (= A259), G245 (= G260), D246 (= D261)
binding phosphoaminophosphonic acid-adenylate ester: T214 (≠ K227), G216 (= G229), V236 (= V250), A244 (= A259), G245 (= G260)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 275

1tz3A Crystal structure of aminoimidazole riboside kinase complexed with aminoimidazole riboside (see paper)
24% identity, 92% coverage: 27:313/313 of query aligns to 19:298/299 of 1tz3A

query
sites
1tz3A

S

N

S

S

Y

Y

D

L

L

K

A
x
C

V

P

A

G

G
|
G

A

A

E

S

F

A

N

N

V

V

A

G

I

V

G

C

T

V

A

A

R

R

L

L

E

G

H

G

K

E

V

C

T

G

Y

F

M

I

T

G

K

C

L

L

G

G

D

D

P

D

F

A

G

G

K

R

R

F

I

L

T

R

T

Q

V

V

L

F

K

Q

D

D

N

N

K

G

I

V

G

D

D

V

E

T

F

F

I

L

S

R

F

L

S

D

K

A

E

D

R

L

A

T

T

S

G

A

F

V

M
x
L

L

I

K

V

G

N

R

S

T
x
F

S

T

V

-

G

-

D

-

P

-

E

-

I

-

F

-

Y
|
Y

F

L

R

V

K

H

N

P

S

G

A

A

A

D

S

T

T

Y

L

V

N

S

E

P

D

Q

D

D

V

L

E

P

K

P

I

F

D

R

L

Q

S

Y

N

E

Y

W

G

F

Y

Y

I

F

H

S

L

S

T

I

G

G

I

L

L

T

P

-

A

-

L

-

S

D

E

R

D

P

T

A

K

R

K

E

A

A

T

C

F

L

S

E

L

G

I

A

K

R

A

M

K

R

K

E

Q

A

G

G

L

G

F

Y

I

V

S

L

F

F

D

D

P

V

N

N

L

L

R
|
R

P

S

Q

K

L
x
M

W

W

K

G

S

N

Q

T

E

D

E

E

M

I

I

P

Q

E

T

L

I

I

N

A

E

R

L

S

A

A

S

A

Y

L

A

A

D

S

L

I

V

C

L

K

P

V

G

S

Q

A

A

D

E

E

G

L

K

C

I

Q

L

L

I

S

G

G

T

A

D

S

D

H

E

W

K

Q

E

D

I

A

N

R

D

-

F

Y

Y

Y

L

L

Q

R

N

D

-

L

G

G

A

C

S

D

I

T

C

T

V

I

T

I

K

S

C

L

G

G

S

A

K

D

G

G

A

A

Y

L

V

L

S

I

T

T

K

A

D

E

G

G

E

E

G

-

Y

F

M

H

V

F

E

P

G

A

Y

P

K

R

V

V

E

D

K

-

V

V

D

D

T

T

V

T

G
|
G

A
|
A

G
|
G

D
|
D

G

A

F

F

A

V

T

G

G

G

V

L

T

F

G

T

L

L

-

S

-

R

-

A

-

N

-

C

M

W

E

D

G

H

L

A

T

L

L

L

S

A

E

E

A

A

V

I

K

S

R

N

G

A

T

N

A

A

I

C

G

G

A

A

I

M

Q

A

V

V

M

T

S

A

R

K

G

G

D

A

N

M

E

T

G

A

L

L

P

P

T

F

R

P

Q

D

E

Q

L

L

K

N

D

T

F

F

M

L

N

S

K

S

active site: C24 (≠ A32), F88 (≠ T96), G238 (= G258), A239 (= A259), G240 (= G260), D241 (= D261)
binding 5-aminoimidazole ribonucleoside: G27 (= G35), L83 (≠ M91), F88 (≠ T96), Y90 (= Y105), R151 (= R169), M154 (≠ L172), D241 (= D261)

Sites not aligning to the query:

binding 5-aminoimidazole ribonucleoside: 8, 10, 12

1tz6A Crystal structure of aminoimidazole riboside kinase from salmonella enterica complexed with aminoimidazole riboside and atp analog (see paper)
24% identity, 91% coverage: 27:312/313 of query aligns to 19:297/297 of 1tz6A

query
sites
1tz6A

S

N

S

S

Y

Y

D

L

L

K

A
x
C

V

P

A

G

G
|
G

A

A

E

S

F

A

N

N

V

V

A

G

I

V

G

C

T

V

A

A

R

R

L

L

E

G

H

G

K

E

V

C

T

G

Y

F

M

I

T

G

K

C

L

L

G

G

D

D

P

D

F

A

G

G

K

R

R

F

I

L

T

R

T

Q

V

V

L

F

K

Q

D

D

N

N

K

G

I

V

G

D

D

V

E

T

F

F

I

L

S

R

F

L

S

D

K

A

E

D

R

L

A

T

T

S

G

A

F

V

M

L

L

I

K

V

G

N

R

S

T
x
F

S

T

V

-

G

-

D

-

P

-

E

-

I

-

F

-

Y
|
Y

F

L

R

V

K

H

N

P

S

G

A

A

A

D

S

T

T

Y

L

V

N

S

E

P

D

Q

D

D

V

L

E

P

K

P

I

F

D

R

L

Q

S

Y

N

E

Y

W

G

F

Y

Y

I

F

H

S

L

S

T

I

G

G

I

L

L

T

P

-

A

-

L

-

S

D

E

R

D

P

T

A

K

R

K

E

A

A

T

C

F

L

S

E

L

G

I

A

K

R

A

M

K

R

K

E

Q

A

G

G

L

G

F

Y

I

V

S

L

F

F

D

D

P

V

N
|
N

L

L

R
|
R

P

S

Q

K

L
x
M

W

W

K

G

S

N

Q

T

E

D

E

E

M

I

I

P

Q

E

T

L

I

I

N

A

E

R

L

S

A

A

S

A

Y

L

A

A

D

S

L

I

V

C

L
x
K

P

V

G

S

Q

A

A

D

E
|
E

G

L

K

C

I

Q

L

L

I

S

G

G

T

A

D

S

D

H

E

W

K

Q

E

D

I

A

N

R

D

-

F

Y

Y

Y

L

L

Q

R

N

D

-

L

G

G

A

C

S

D

I

T

C

T

V

I

T

I

K
x
S

C

L

G
|
G

S
x
A

K

D

G
|
G

A

A

Y

L

V

L

S

I

T

T

K

A

D

E

G

G

E

E

G

-

Y

F

M

H

V

F

E

P

G

A

Y

P

K

R

V

V

E

D

K

-

V

V

D

D

T

T

V

T

G
|
G

A
|
A

G
|
G

D
|
D

G

A

F
|
F

A

V

T

G

G

G

V

L

T

F

G

T

L

L

-

S

-

R

-

A

-

N

-

C

M

W

E

D

G

H

L

A

T

L

L

L

S

A

E

E

A

A

V

I

K

S

R

N

G

A

T
x
N

A

A

I

C

G
|
G

A
|
A

I

M

Q

A

V

V

M

T

S

A

R

K

G

G

D

A

N

M

E

T

G

A

L

L

P

P

T

F

R

P

Q

D

E

Q

L

L

K

N

D

T

F

F

M

L

N

S

active site: C24 (≠ A32), F88 (≠ T96), G238 (= G258), A239 (= A259), G240 (= G260), D241 (= D261)
binding phosphomethylphosphonic acid adenylate ester: N149 (= N167), K176 (≠ L194), E181 (= E199), S209 (≠ K227), G211 (= G229), A212 (≠ S230), G214 (= G232), A239 (= A259), G240 (= G260), F243 (= F263), N270 (≠ T285), G273 (= G288), A274 (= A289)
binding 5-aminoimidazole ribonucleoside: G27 (= G35), F88 (≠ T96), Y90 (= Y105), R151 (= R169), M154 (≠ L172), D241 (= D261)

Sites not aligning to the query:

binding 5-aminoimidazole ribonucleoside: 8, 12

4eumA Crystal structure of a sugar kinase (target efi-502132) from oceanicola granulosus with bound amp, crystal form ii
29% identity, 80% coverage: 28:277/313 of query aligns to 35:278/294 of 4eumA

query
sites
4eumA

S

T

Y

Y

D

R

L

L

A

G

V

F

A

A

G

G

A

D

E

T

F

F

N

N

V

T

A

A

I

W

G

Y

T

L

A

A

R

R

L

L

-

R

-

P

E

E

H

S

K

R

V

I

T

S

Y

Y

M

F

T

S

K

A

L

I

G

G

D

D

P

A

F

L

G

S

K

Q

R

Q

I

M

T

R

T

A

V

A

L

M

K

S

D

A

N

A

K

G

I

I

G

D

D

G

E

G

F

G

I

L

S

R

F

V

S

I

K

P

E

G

R

R

A

T

T

V

G

G

F

L

M

Y

L

L

K

-

G

-

R

-

T

-

S

-

V

-

G

-

D

-

P

-

E

-

I

I

F

F

-

A

Y

Y

F

W

R

R

K

G

N

Q

S

S

A

A

S

R

T

E

L

L

N

A

E

G

D

D

D

A

V

D

E

A

K

L

I

A

D

A

L

A

S

M

N

A

Y

R

G

A