SitesBLAST
Comparing WP_008539135.1 NCBI__GCF_000245775.1:WP_008539135.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
2f02A Crystal structure of lacc from enterococcus faecalis in complex with atp
28% identity, 100% coverage: 1:303/303 of query aligns to 2:306/319 of 2f02A
- binding adenosine-5'-triphosphate: N186 (= N183), S224 (= S219), G226 (= G221), I243 (≠ V239), I246 (≠ G242), G253 (= G249), S254 (≠ A250), G255 (= G251), T258 (≠ M254), M280 (≠ V276), G283 (= G279), M284 (≠ S280)
2jgvB Structure of staphylococcus aureus d-tagatose-6-phosphate kinase in complex with adp (see paper)
31% identity, 87% coverage: 1:263/303 of query aligns to 5:269/314 of 2jgvB
Sites not aligning to the query:
2jg1A Structure of staphylococcus aureus d-tagatose-6-phosphate kinase with cofactor and substrate (see paper)
30% identity, 87% coverage: 1:263/303 of query aligns to 9:273/318 of 2jg1A
- active site: G259 (= G249), S260 (≠ A250), G261 (= G251), D262 (= D252)
- binding phosphoaminophosphonic acid-adenylate ester: K46 (= K38), N193 (= N183), S230 (= S219), G232 (= G221), G235 (= G224), I252 (≠ G242), V254 (≠ L244), G259 (= G249), S260 (≠ A250), G261 (= G251), D262 (= D252), T264 (≠ M254)
Sites not aligning to the query:
2jg1C Structure of staphylococcus aureus d-tagatose-6-phosphate kinase with cofactor and substrate (see paper)
30% identity, 87% coverage: 1:263/303 of query aligns to 6:270/315 of 2jg1C
- active site: G256 (= G249), S257 (≠ A250), G258 (= G251), D259 (= D252)
- binding phosphoaminophosphonic acid-adenylate ester: S227 (= S219), G229 (= G221), A230 (≠ K222), G232 (= G224), I246 (≠ V239), I249 (≠ G242), V251 (≠ L244), V255 (≠ I248), G256 (= G249), S257 (≠ A250), G258 (= G251), D259 (= D252), T261 (≠ M254)
- binding 6-O-phosphono-beta-D-tagatofuranose: D17 (= D12), G42 (= G37), K43 (= K38), R93 (= R89), C95 (≠ N91), L108 (≠ N104), G140 (= G135), S141 (= S136), D259 (= D252)
Sites not aligning to the query:
3uqeA Crystal structure of the phosphofructokinase-2 mutant y23d from escherichia coli
27% identity, 100% coverage: 2:303/303 of query aligns to 4:307/307 of 3uqeA
- active site: G253 (= G249), A254 (= A250), G255 (= G251), D256 (= D252)
- binding adenosine-5'-triphosphate: K185 (= K181), N187 (= N183), S224 (= S219), G226 (= G221), P227 (≠ K222), G229 (= G224), S248 (≠ L244), A254 (= A250), G255 (= G251), M258 (= M254), V280 (= V276), G283 (= G279), S284 (= S280), T287 (≠ A283)
- binding pyrophosphate 2-: N187 (= N183), K189 (≠ H185)
3uqdB Crystal structure of the phosphofructokinase-2 from escherichia coli in complex with substrates and products (see paper)
29% identity, 96% coverage: 2:291/303 of query aligns to 4:293/309 of 3uqdB
- active site: G253 (= G249), A254 (= A250), G255 (= G251), D256 (= D252)
- binding adenosine-5'-diphosphate: K185 (= K181), N187 (= N183), S224 (= S219), G226 (= G221), P227 (≠ K222), G229 (= G224), S248 (≠ L244), M258 (= M254), V280 (= V276), G283 (= G279), S284 (= S280)
- binding adenosine-5'-triphosphate: Y23 (≠ I21), K27 (≠ T25)
- binding 6-O-phosphono-beta-D-fructofuranose: K27 (≠ T25), R29 (= R27)
- binding 1,6-di-O-phosphono-beta-D-fructofuranose: D14 (= D12), G39 (= G37), N43 (= N41), R90 (= R89), R105 (≠ E102), S139 (= S136), G253 (= G249)
3uqdA Crystal structure of the phosphofructokinase-2 from escherichia coli in complex with substrates and products (see paper)
29% identity, 96% coverage: 2:291/303 of query aligns to 4:293/309 of 3uqdA
- active site: G253 (= G249), A254 (= A250), G255 (= G251), D256 (= D252)
- binding adenosine-5'-triphosphate: K185 (= K181), S224 (= S219), G226 (= G221), P227 (≠ K222), G229 (= G224), T251 (≠ S247), G255 (= G251), M258 (= M254), V280 (= V276), G283 (= G279), S284 (= S280), T287 (≠ A283)
- binding 6-O-phosphono-beta-D-fructofuranose: D14 (= D12), G38 (= G36), G39 (= G37), N43 (= N41), R90 (= R89), S139 (= S136), D256 (= D252)
- binding 1,6-di-O-phosphono-beta-D-fructofuranose: K27 (≠ T25), R29 (= R27)
3n1cA Crystal structure of the phosphofructokinase-2 from escherichia coli in complex with fructose-6-phosphate (see paper)
29% identity, 96% coverage: 2:291/303 of query aligns to 4:293/309 of 3n1cA
- active site: G253 (= G249), A254 (= A250), G255 (= G251), D256 (= D252)
- binding 6-O-phosphono-beta-D-fructofuranose: D14 (= D12), K27 (≠ T25), R29 (= R27), G39 (= G37), N43 (= N41), R90 (= R89), G138 (= G135), S139 (= S136), D256 (= D252)
P06999 ATP-dependent 6-phosphofructokinase isozyme 2; ATP-PFK 2; Phosphofructokinase 2; 6-phosphofructokinase isozyme II; Phosphohexokinase 2; EC 2.7.1.11 from Escherichia coli (strain K12) (see 3 papers)
29% identity, 96% coverage: 2:291/303 of query aligns to 4:293/309 of P06999
- K27 (≠ T25) binding ATP; binding ATP
- KPN 185:187 (= KPN 181:183) binding in other chain
- NQK 187:189 (≠ NNH 183:185) binding in other chain
- E190 (= E186) mutation to Q: Causes a 50-fold decrease in the kcat value and a 15-fold increment in the apparent KM for ATP.
- SLGPQG 224:229 (≠ SMGKDG 219:224) binding in other chain
- S248 (≠ L244) binding in other chain
- S250 (≠ N246) binding K(+)
- V252 (≠ I248) binding K(+)
- V280 (= V276) binding in other chain
- S284 (= S280) binding in other chain
- A286 (≠ S282) binding K(+)
- N289 (= N287) binding K(+)
- G291 (≠ A289) binding K(+)
- R293 (= R291) binding K(+)
3cqdA Structure of the tetrameric inhibited form of phosphofructokinase-2 from escherichia coli (see paper)
28% identity, 93% coverage: 2:284/303 of query aligns to 4:288/304 of 3cqdA
- active site: G253 (= G249), A254 (= A250), G255 (= G251), D256 (= D252)
- binding adenosine-5'-triphosphate: Y23 (≠ I21), G26 (≠ A24), K27 (≠ T25), K185 (= K181), N187 (= N183), N187 (= N183), K189 (≠ H185), S224 (= S219), G226 (= G221), P227 (≠ K222), G229 (= G224), S248 (≠ L244), T251 (≠ S247), A254 (= A250), G255 (= G251), M258 (= M254), V280 (= V276), G283 (= G279), S284 (= S280), T287 (≠ A283)
2ajrA Crystal structure of possible 1-phosphofructokinase (ec 2.7.1.56) (tm0828) from thermotoga maritima at 2.46 a resolution
29% identity, 97% coverage: 1:293/303 of query aligns to 2:297/320 of 2ajrA
Sites not aligning to the query:
P9WID3 ATP-dependent 6-phosphofructokinase isozyme 2; ATP-PFK 2; Phosphofructokinase 2; Phosphofructokinase B; Phosphohexokinase 2; Tagatose-6-phosphate kinase; EC 2.7.1.11; EC 2.7.1.144 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
27% identity, 97% coverage: 2:295/303 of query aligns to 14:310/339 of P9WID3
- K283 (= K270) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
3ie7A The crystal structure of phosphofructokinase (lin2199) from listeria innocua in complex with atp at 1.6a
27% identity, 100% coverage: 1:303/303 of query aligns to 2:302/309 of 3ie7A
- binding adenosine-5'-triphosphate: N188 (= N183), S220 (= S219), G222 (= G221), A223 (≠ K222), G225 (= G224), V242 (≠ G242), G249 (= G249), A250 (= A250), G251 (= G251), D252 (= D252), S279 (= S280), V283 (≠ F284)
3julA Crystal structure of listeria innocua d-tagatose-6-phosphate kinase bound with substrate
27% identity, 86% coverage: 1:261/303 of query aligns to 2:254/298 of 3julA
A1A6H3 Ribokinase; AtRBSK; RK; EC 2.7.1.15 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
23% identity, 88% coverage: 14:280/303 of query aligns to 85:353/379 of A1A6H3