SitesBLAST
Comparing WP_008540147.1 NCBI__GCF_000245775.1:WP_008540147.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1wwkA Crystal structure of phosphoglycerate dehydrogenase from pyrococcus horikoshii ot3
42% identity, 93% coverage: 3:306/326 of query aligns to 2:304/304 of 1wwkA
- active site: S96 (≠ N96), R230 (= R232), D254 (= D256), E259 (= E261), H278 (= H280)
- binding nicotinamide-adenine-dinucleotide: V100 (= V100), G146 (= G147), F147 (≠ C148), G148 (= G149), R149 (≠ H150), I150 (= I151), Y168 (= Y169), D169 (= D170), P170 (= P171), V201 (≠ T203), P202 (= P204), T207 (= T209), T228 (≠ C230), S229 (≠ A231), D254 (= D256), H278 (= H280), G280 (≠ A282)
8wpiA Anabaena mcyi with prebound NAD and soaked NADP
36% identity, 97% coverage: 3:318/326 of query aligns to 1:318/318 of 8wpiA
- binding nicotinamide-adenine-dinucleotide: V99 (= V100), G146 (= G147), G148 (= G149), R149 (≠ H150), I150 (= I151), Y169 (= Y169), D170 (= D170), P171 (= P171), Y172 (≠ F172), H202 (= H202), P203 (≠ T203), E204 (≠ P204), E208 (≠ K208), T209 (= T209), T230 (≠ C230), S231 (≠ A231), R232 (= R232), H280 (= H280)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: Y9 (≠ H11), Y48 (≠ I50), S69 (≠ P70), G70 (= G71), F71 (≠ V72), G72 (= G73), L110 (≠ C111), K113 (≠ D114), F116 (≠ R117), F226 (≠ I226), R232 (= R232), D273 (≠ E273), N274 (= N274), V284 (≠ Q284), T285 (= T285), G289 (≠ A289)
8wprA Anabaena mcyi r166a with prebound NAD and malate
36% identity, 97% coverage: 3:318/326 of query aligns to 3:320/320 of 8wprA
- binding nicotinamide-adenine-dinucleotide: S97 (≠ N96), V101 (= V100), G150 (= G149), A151 (≠ H150), I152 (= I151), Y171 (= Y169), D172 (= D170), P173 (= P171), Y174 (≠ F172), P205 (≠ T203), E206 (≠ P204), E210 (≠ K208), T211 (= T209), T232 (≠ C230), S233 (≠ A231), R234 (= R232), H282 (= H280), G285 (= G283)
5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
35% identity, 94% coverage: 3:307/326 of query aligns to 4:315/334 of 5aovA
- active site: L100 (≠ N96), R241 (= R232), D265 (= D256), E270 (= E261), H288 (= H280)
- binding glyoxylic acid: M52 (≠ R49), L53 (≠ I50), L53 (≠ I50), Y74 (≠ P70), A75 (≠ G71), V76 (= V72), G77 (= G73), R241 (= R232), H288 (= H280)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (= V72), T104 (≠ V100), F158 (≠ C148), G159 (= G149), R160 (≠ H150), I161 (= I151), S180 (≠ D170), R181 (≠ P171), A211 (≠ H202), V212 (≠ T203), P213 (= P204), T218 (= T209), I239 (≠ C230), A240 (= A231), R241 (= R232), H288 (= H280), G290 (≠ A282)
O43175 D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; Malate dehydrogenase; EC 1.1.1.95; EC 1.1.1.399; EC 1.1.1.37 from Homo sapiens (Human) (see 3 papers)
35% identity, 97% coverage: 1:317/326 of query aligns to 6:320/533 of O43175
- T78 (≠ V72) binding NAD(+)
- R135 (= R130) to W: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606949
- RI 155:156 (≠ HI 150:151) binding NAD(+)
- D175 (= D170) binding NAD(+)
- T207 (= T203) binding NAD(+)
- CAR 234:236 (= CAR 230:232) binding NAD(+)
- D260 (= D256) binding NAD(+)
- V261 (= V257) to M: in PHGDHD; results in a four-fold decrease in substrate affinity and a slight increase in maximal enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs267606947
- HLGA 283:286 (≠ HMAG 280:283) binding NAD(+)
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
- 373 A → T: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs201553627
- 377 G → S: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606948
- 425 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907988
- 490 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907987
7ewhA Crystal structure of human phgdh in complex with homoharringtonine (see paper)
36% identity, 89% coverage: 1:290/326 of query aligns to 1:288/302 of 7ewhA
- binding (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine: L146 (≠ I146), G147 (= G147), L148 (≠ C148), G149 (= G149), R150 (≠ H150), I151 (= I151), G152 (= G152), D170 (= D170), H201 (= H202), T202 (= T203), P203 (= P204)
6rihA Crystal structure of phgdh in complex with compound 9 (see paper)
36% identity, 89% coverage: 1:290/326 of query aligns to 1:288/302 of 6rihA
6plgA Crystal structure of human phgdh complexed with compound 15 (see paper)
36% identity, 89% coverage: 1:290/326 of query aligns to 1:288/303 of 6plgA
6rj5A Crystal structure of phgdh in complex with compound 39 (see paper)
36% identity, 89% coverage: 1:290/326 of query aligns to 1:288/301 of 6rj5A
6plfA Crystal structure of human phgdh complexed with compound 1 (see paper)
36% identity, 89% coverage: 1:290/326 of query aligns to 2:289/305 of 6plfA
7dkmA Phgdh covalently linked to oridonin (see paper)
36% identity, 89% coverage: 1:290/326 of query aligns to 2:289/306 of 7dkmA
- binding nicotinamide-adenine-dinucleotide: T74 (≠ V72), A102 (≠ V100), G148 (= G147), R151 (≠ H150), I152 (= I151), Y170 (= Y169), D171 (= D170), P172 (= P171), I173 (≠ F172), H202 (= H202), T203 (= T203), P204 (= P204), T209 (= T209), C230 (= C230), A231 (= A231), R232 (= R232), H279 (= H280), G281 (≠ A282)
- binding (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14R,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one: C14 (≠ D13), K17 (≠ A16), I18 (≠ V17)
Sites not aligning to the query:
- binding (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14R,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one: 293
6cwaA Crystal structure phgdh in complex with nadh and 3-phosphoglycerate at 1.77 a resolution (see paper)
36% identity, 88% coverage: 3:290/326 of query aligns to 2:287/299 of 6cwaA
- binding 1,4-dihydronicotinamide adenine dinucleotide: N96 (= N96), A100 (≠ V100), R149 (≠ H150), I150 (= I151), Y168 (= Y169), D169 (= D170), P170 (= P171), I171 (≠ F172), H200 (= H202), T201 (= T203), P202 (= P204), T207 (= T209), C228 (= C230), A229 (= A231), R230 (= R232), H277 (= H280), G279 (≠ A282)
6rj3A Crystal structure of phgdh in complex with compound 15 (see paper)
36% identity, 88% coverage: 3:290/326 of query aligns to 2:287/297 of 6rj3A
6rj2A Crystal structure of phgdh in complex with compound 40 (see paper)
36% identity, 88% coverage: 4:290/326 of query aligns to 1:285/299 of 6rj2A
- binding ~{N}-[(1~{R})-1-[4-(ethanoylsulfamoyl)phenyl]ethyl]-2-methyl-5-phenyl-pyrazole-3-carboxamide: G146 (= G149), I148 (= I151), Y166 (= Y169), D167 (= D170), P168 (= P171), I169 (≠ F172), I170 (≠ V173), H198 (= H202), T199 (= T203), L208 (≠ M212), R228 (= R232)
6plfB Crystal structure of human phgdh complexed with compound 1 (see paper)
37% identity, 88% coverage: 3:290/326 of query aligns to 2:279/292 of 6plfB