SitesBLAST
Comparing WP_008636643.1 NCBI__GCF_000224335.1:WP_008636643.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1wwkA Crystal structure of phosphoglycerate dehydrogenase from pyrococcus horikoshii ot3
32% identity, 98% coverage: 1:313/318 of query aligns to 1:298/304 of 1wwkA
- active site: S96 (≠ N96), R230 (= R227), D254 (= D251), E259 (≠ N262), H278 (= H293)
- binding nicotinamide-adenine-dinucleotide: V100 (= V100), G146 (= G146), F147 (≠ Y147), G148 (= G148), R149 (≠ N149), I150 (≠ M150), Y168 (= Y168), D169 (= D169), P170 (≠ I170), V201 (≠ T198), P202 (= P199), T207 (= T204), T228 (= T225), S229 (≠ A226), D254 (= D251), H278 (= H293), G280 (≠ A295)
6cwaA Crystal structure phgdh in complex with nadh and 3-phosphoglycerate at 1.77 a resolution (see paper)
30% identity, 97% coverage: 2:309/318 of query aligns to 2:293/299 of 6cwaA
- binding 1,4-dihydronicotinamide adenine dinucleotide: N96 (= N96), A100 (≠ V100), R149 (≠ N149), I150 (≠ M150), Y168 (= Y168), D169 (= D169), P170 (vs. gap), I171 (vs. gap), H200 (= H197), T201 (= T198), P202 (= P199), T207 (= T204), C228 (≠ T225), A229 (= A226), R230 (= R227), H277 (= H293), G279 (≠ A295)
7ewhA Crystal structure of human phgdh in complex with homoharringtonine (see paper)
30% identity, 97% coverage: 2:309/318 of query aligns to 3:294/302 of 7ewhA
- binding (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine: L146 (≠ I145), G147 (= G146), L148 (≠ Y147), G149 (= G148), R150 (≠ N149), I151 (≠ M150), G152 (= G151), D170 (= D169), H201 (= H197), T202 (= T198), P203 (= P199)
6rihA Crystal structure of phgdh in complex with compound 9 (see paper)
30% identity, 97% coverage: 2:309/318 of query aligns to 3:294/302 of 6rihA
6rj3A Crystal structure of phgdh in complex with compound 15 (see paper)
30% identity, 97% coverage: 2:309/318 of query aligns to 2:293/297 of 6rj3A
6rj5A Crystal structure of phgdh in complex with compound 39 (see paper)
30% identity, 97% coverage: 2:309/318 of query aligns to 3:294/301 of 6rj5A
6plgA Crystal structure of human phgdh complexed with compound 15 (see paper)
30% identity, 97% coverage: 2:309/318 of query aligns to 3:294/303 of 6plgA
7dkmA Phgdh covalently linked to oridonin (see paper)
30% identity, 97% coverage: 2:309/318 of query aligns to 4:295/306 of 7dkmA
- binding nicotinamide-adenine-dinucleotide: T74 (≠ A72), A102 (≠ V100), G148 (= G146), R151 (≠ N149), I152 (≠ M150), Y170 (= Y168), D171 (= D169), P172 (vs. gap), I173 (vs. gap), H202 (= H197), T203 (= T198), P204 (= P199), T209 (= T204), C230 (≠ T225), A231 (= A226), R232 (= R227), H279 (= H293), G281 (≠ A295)
- binding (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14R,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one: C14 (≠ F12), K17 (≠ E15), I18 (≠ Q16), E293 (≠ Q307)
6plfA Crystal structure of human phgdh complexed with compound 1 (see paper)
30% identity, 97% coverage: 2:309/318 of query aligns to 4:295/305 of 6plfA
O43175 D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; Malate dehydrogenase; EC 1.1.1.95; EC 1.1.1.399; EC 1.1.1.37 from Homo sapiens (Human) (see 3 papers)
30% identity, 97% coverage: 2:309/318 of query aligns to 8:299/533 of O43175
- T78 (≠ A72) binding NAD(+)
- R135 (= R129) to W: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606949
- RI 155:156 (≠ NM 149:150) binding NAD(+)
- D175 (= D169) binding NAD(+)
- T207 (= T198) binding NAD(+)
- CAR 234:236 (≠ TAR 225:227) binding NAD(+)
- D260 (= D251) binding NAD(+)
- V261 (= V252) to M: in PHGDHD; results in a four-fold decrease in substrate affinity and a slight increase in maximal enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs267606947
- HLGA 283:286 (≠ HVAG 293:296) binding NAD(+)
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
- 373 A → T: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs201553627
- 377 G → S: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606948
- 425 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907988
- 490 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907987
6rj2A Crystal structure of phgdh in complex with compound 40 (see paper)
29% identity, 97% coverage: 3:309/318 of query aligns to 1:291/299 of 6rj2A
- binding ~{N}-[(1~{R})-1-[4-(ethanoylsulfamoyl)phenyl]ethyl]-2-methyl-5-phenyl-pyrazole-3-carboxamide: G146 (= G148), I148 (≠ M150), Y166 (= Y168), D167 (= D169), P168 (vs. gap), I169 (vs. gap), I170 (= I170), H198 (= H197), T199 (= T198), L208 (≠ M207), R228 (= R227)
P87228 Putative D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; EC 1.1.1.95; EC 1.1.1.399 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
33% identity, 95% coverage: 1:302/318 of query aligns to 56:353/466 of P87228
- S87 (= S31) modified: Phosphoserine
- S258 (≠ T201) modified: Phosphoserine
1ybaA The active form of phosphoglycerate dehydrogenase (see paper)
32% identity, 98% coverage: 1:313/318 of query aligns to 7:308/406 of 1ybaA
- active site: N104 (= N96), R236 (= R227), D260 (= D251), E265 (= E256), H288 (= H293)
- binding 2-oxoglutaric acid: R56 (= R48), S57 (= S49), C79 (≠ G71), I80 (≠ A72)
- binding nicotinamide-adenine-dinucleotide: I80 (≠ A72), F102 (≠ E94), V108 (= V100), G154 (= G146), G156 (= G148), H157 (≠ N149), I158 (≠ M150), Y176 (= Y168), D177 (= D169), I178 (= I170), K181 (≠ N173), H206 (= H197), V207 (≠ T198), P208 (= P199), A234 (≠ T225), S235 (≠ A226), R236 (= R227), H288 (= H293), G290 (≠ A295)
- binding phosphate ion: G81 (= G73), N83 (≠ E75)
1psdA The allosteric ligand site in the vmax-type cooperative enzyme phosphoglycerate dehydrogenase (see paper)
32% identity, 98% coverage: 1:313/318 of query aligns to 5:306/404 of 1psdA
- active site: N102 (= N96), R234 (= R227), D258 (= D251), E263 (= E256), H286 (= H293)
- binding nicotinamide-adenine-dinucleotide: N102 (= N96), H155 (≠ N149), I156 (≠ M150), D175 (= D169), I176 (= I170), K179 (≠ N173), H204 (= H197), V205 (≠ T198), P206 (= P199), A232 (≠ T225), S233 (≠ A226), R234 (= R227), H286 (= H293)
Sites not aligning to the query:
P0A9T0 D-3-phosphoglycerate dehydrogenase; PGDH; 2-oxoglutarate reductase; EC 1.1.1.95; EC 1.1.1.399 from Escherichia coli (strain K12) (see 2 papers)
32% identity, 98% coverage: 1:313/318 of query aligns to 11:312/410 of P0A9T0