SitesBLAST

M1 (= M1) modified: Initiator methionine, Removed
Y102 (= Y102) mutation to L: Retains 4% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
W106 (= W106) mutation to L: Retains 20% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
K163 (= K163) mutation to A: Retains 24% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
F216 (= F216) mutation to L: Retains 13% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
E222 (= E222) mutation to A: Abolishes lysine uptake. Strongly inhibits CadC.
E230 (= E230) mutation to V: Abolishes lysine uptake. Shows significant less inhibition of CadC.
D275 (= D275) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-278.
D278 (= D278) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-275.
E438 (= E438) mutation to A: Retains 14% of wild-type lysine uptake activity. Is unable to inhibit CadC.
D443 (= D443) mutation to A: Retains 11% of wild-type lysine uptake activity. Is unable to inhibit CadC.
D446 (= D446) mutation to A: Retains 13% of wild-type lysine uptake activity. Is unable to inhibit CadC.

P46349 Gamma-aminobutyric acid permease; GABA permease; 4-aminobutyrate permease; Gamma-aminobutyrate permease; Proline transporter GabP from Bacillus subtilis (strain 168) (see paper)
43% identity, 83% coverage: 13:418/489 of query aligns to 8:402/469 of P46349

query
sites
P46349

L

L

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G33 (≠ A38) mutation to D: Lack of activity.
G42 (= G47) mutation to S: Lack of activity.
G301 (= G315) mutation to V: Lack of activity.
G338 (≠ T352) mutation to E: Lack of activity.
F341 (≠ I355) mutation to S: Lack of activity.

Sites not aligning to the query:

414 G→R: Lack of activity.

P04817 Arginine permease CAN1; Canavanine resistance protein 1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
38% identity, 95% coverage: 13:476/489 of query aligns to 84:553/590 of P04817

query
sites
P04817

L

V

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P113 (≠ T42) mutation to L: In CAN1-343; confers citrulline transport activity in GAP1-deleted cells.
P148 (= P77) mutation to L: In CAN1-337; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, but not sensitivity to L-aspartic acid alpha-hydroxamate or p-fluoro-L-phenylalanine.
V149 (= V78) mutation to F: In CAN1-315; confers citrulline transport activity in GAP1-deleted cells.
S152 (= S81) mutation to F: In CAN1-342; confers citrulline transport activity in GAP1-deleted cells.
Y173 (= Y102) mutation to D: In CAN1-306; confers citrulline transport activity in GAP1-deleted cells.; mutation to H: In CAN1-327; confers citrulline transport activity in GAP1-deleted cells.
G308 (= G229) mutation to A: In CAN1-341; confers citrulline transport activity in GAP1-deleted cells.
P313 (= P234) mutation to S: In CAN1-329; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, L-aspartic acid alpha-hydroxamate and p-fluoro-L-phenylalanine.
TS 354:355 (≠ VK 276:277) mutation Missing: In CAN1-318; confers citrulline transport activity in GAP1-deleted cells.
Y356 (≠ D278) mutation to H: In CAN1-340; confers citrulline transport activity in GAP1-deleted cells.; mutation to N: In CAN1-339; confers citrulline transport activity in GAP1-deleted cells.
W451 (= W373) mutation to C: In CAN1-328; confers citrulline transport activity in GAP1-deleted cells.; mutation to L: In CAN1-316; confers citrulline transport activity in GAP1-deleted cells.; mutation to S: In CAN1-335; confers citrulline transport activity in GAP1-deleted cells.
F461 (= F383) mutation to S: In CAN1-307; confers citrulline transport activity in GAP1-deleted cells.

P19145 General amino-acid permease GAP1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
37% identity, 95% coverage: 7:471/489 of query aligns to 81:551/602 of P19145

query
sites
P19145

T

T

T

A

E

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A

T

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P

T

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L

L

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R

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E

H

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F

F

W

W

R

R

I

I

L

T

L

L

F

F

Y

Y

V

I

F

L

A

S

I

L

L

L

I

M

I

I

S

G

L

L

I

L

I

V

P

P

Y

Y

T

N

D

D

P

K

S

S

L

L

L

I

R

G

N

A

D

S

V

S

K

V

D

D

I

A

S

A

V

A

S

S

P

P

F

F

T

V

L

I

V

A

F

I

Q

K

H

T

A

H

G

G

L

I

L

K

S

G

A

L

A

P

A

S

I

V

M

V

N

N

A

V

V

V

I

I

L

L

T

I

A

A

V

V

L

L

S

S

A

V

G

G

N

N

S

S

G

A

M

I

Y

Y

A

A

S

C

T

S

R

R

M

T

L

M

Y

V

T

A

L

L

A

A

C

E

D

Q

G

R

K

F

A

L

P

P

R

E

I

I

F

F

S

S

K

Y

L

V

S

D

R

R

G

K

G

G

V

R

P

P

R

L

N

V

A

G

L

I

Y

A

A

V

T

T

T

S

V

A

I

F

A

G

A

L

L

I

C

A

F

F

L

V

T

A

S

A

M

S

F

K

G

K

N

E

Q

G

T

E

V

V

Y

F

L

N

W

W

L

L

N

A

T

L

S

S

G

G

M

L

T

S

G

S

F

L

I

F

A

T

W

W

L

G

G

G

I

I

A

C

I

I

S

C

H

H

Y

I

R

R

F

F

R

R

R

K

G

A

Y

L

V

A

M

A

Q

Q

G

G

N

R

D

G

I

L

N

D

N

E

L

L

P

S

Y

F

R

K

S

S

G

P

F

T

F

G

P

V

L

W

G

G

P

S

I

Y

F

W

A

G

F

L

V

F

L

M

C

V

L

I

I

I

I

M

T

F

L

I

G

A

Q

Q

N

F

Y

Y

E

V

A

A

F

V

L

F

K

P

-

V

-

G

D

D

T

S

I

P

D

S

W

A

G

E

G

G

V

F

A

F

A

E

T

A

Y

Y

I

L

G

S

I

F

P

P

L

L

F

V

L

M

V

V

I

M

W

Y

F

I

G

G

Y

H

K

K

L

I

A

Y

K

K

A297 (≠ Q219) mutation to V: Impairs basic amino-acids transport and regulation by these amino-acids.

Sites not aligning to the query:

76 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Q9URZ4 Cationic amino acid transporter 1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
35% identity, 94% coverage: 9:469/489 of query aligns to 76:540/587 of Q9URZ4

query
sites
Q9URZ4

E

D

A

G

P

T

T

A

L

L

R

K

R

R

E

S

L

L

K

K

A

S

R

R

H

H

L

M

T

Q

M

M

I

I

A

S

I

I

G

G

S

A

I

I

G

G

T

T

G

G

L

L

F

Y

V

V

A

G

S

S

G

G

A

S

T

S

I

L

S

A

Q

D

A

G

G

G

P

P

G

A

G

S

A

V

L

I

S

N

Y

Y

I

S

L

L

I

I

G

G

L

I

M

M

V

M

Y

F

F

F

L

I

M

V

T

Y

S

A

L

L

G

G

E

E

L

M

A

A

A

V

F

A

M

Y

P

P

V

V

S

A

G

G

S

G

F

F

A

N

T

T

Y

Y

G

A

Q

T

N

R

Y

F

V

I

E

D

E

P

G

A

F

W

G

G

F

F

A

A

L

V

G

S

W

W

N

N

Y

Y

W

F

Y

I

N

N

W

Y

A

F

V

V

T

T

I

F

A

P

V

L

D

E

L

L

V

T

A

T

S

C

Q

A

L

I

V

T

M

F

S

R

Y

Y

W

W

F

-

P

T

D

D

T

I

P

N

G

S

W

A

I

A

W

W

S

I

A

S

L

I

F

F

L

L

G

V

I

V

M

I

F

I

L

I

L

V

N

N

W

L

I

F

S

G

V

V

R

R

G

A

F

Y

G

G

E

E

A

V

E

E

Y

F

W

I

F

L

S

S

L

T

I

V

K

K

V

V

A

V

T

A

V

T

I

F

I

G

F

F

I

I

V

L

G

A

V

I

M

I

M

-

I

-

V

-

G

-

I

I

F

N

K

C

G

G

S

G

Q

V

P

P

T

T

G

D

W

H

S

R

N

G

W

Y

-

I

-

G

-

S

G

I

I

I

A

K

D

H

A

K

P

P

F

F

A

R

G

H

G

G

F

F

S

K

A

G

M

F

I

C

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S

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V

A

F

M

T

I

T

V

A

G

A

F

F

S

S

F

F

Q

S

G

G

T

T

E

E

L

V

I

I

G

G

I

L

A

A

G

A

E

E

S

V

E

D

D

N

P

P

E

Q

K

K

N

A

I

L

P

P

R

H

A

A

V

V

R

K

Q

Q

V

V

F

F

W

W

R

R

I

I

L

A

L

I

F

F

Y

Y

V

V

F

V

A

S

I

L

L

I

I

L

I

I

S

G

L

L

I

L

I

I

P

S

Y

P

T

D

D

D

P

P

S

N

L

L

L

M

R

G

N

N

D

G

V

-

K

-

D

S

I

T

S

S

V

V

S

S

P

P

F

F

T

V

L

L

V

A

F

I

Q

K

H

E

A

A

G

N

L

I

L

K

S

G

A

L

A

P

A

S

I

V

M

F

N

N

A

A

V

V

I

I

L

I

T

I

A

S

V

V

L

V

S

S

A

V

G

T

N

N

S

S

G

S

M

T

Y

Y

A

T

S

A

T

G

R

R

M

T

L

L

Y

H

T

G

L

M

A

A

C

N

D

L

G

K

K

Q

A

A

P

P

R

S

I

F

F

F

S

K

K

Y

L

T

S

D

R

R

G

L

G

G

V

R

P

P

R

L

N

L

A

A

L

M

Y

I

A

V

T

V

T

L

V

L

I

F

A

G

A

F

L

F

C

A

F

Y

L

I

T

N

S

E

M

A

F

D

G

K

N

N

-

G

-

N

-

D

-

V

-

S

Q

D

T

T

V

V

Y

F

L

N

W

W

L

L

N

A

T

L

S

S

G

G

M

L

T

S

G

N

F

F

I

F

A

T

W

W

L

G

G

S

I

I

A

C

I

L

S

C

H

H

Y

I

R

I

F

F

R

R

R

L

G

A

Y

F

V

K

M

K

Q

Q

G

G

N

H

D

S

I

L

N

K

N

E

L

L

P

G

Y

F

R

V

S

S

G

-

F

-

P

P

L

M

G

G

-

I

-

W

-

G

-

S

-

C

-

I

P

G

I

L

F

F

A

F

F

N

V

I

L

L

C

C

L

L

I

-

T

-

L

M

G

A

Q

Q

N

F

Y

Y

E

V

A

S

F

L

-

F

-

P

L

I

K

G

D

G

T

K

I

P

D

N

W

A

G

N

G

D

V

F

A

F

A

Q

T

G

Y

Y

I

L

G

A

I

A

P

P

L

V

F

T

L

L

V

A

I

F

W

F

F

I

G

G

Y

Y

K

K

L

I

Sites not aligning to the query:

29 modified: Phosphoserine
30 modified: Phosphoserine
37 modified: Phosphoserine

P24207 Phenylalanine-specific permease; Phenylalanine:H(+) symporter PheP from Escherichia coli (strain K12) (see 3 papers)
38% identity, 85% coverage: 3:420/489 of query aligns to 9:413/458 of P24207

query
sites
P24207

S

E

E

D

T

T

K

A

T

S

T

N

E

Q

A

E

P

P

T

T

L

L

R

H

R

R

E

G

L

L

K

H

A

N

R
|
R

H

H

L

I

T

Q

M

L

I

I

A

A

I

L

G

G

S

A

I

I

G

G

T

T

G

G

L

L

F

F

V

L

A

G

S

I

G

G

A

P

T

A

I

I

S

Q

Q

M

A

A

G

G

P
|
P

G

A

G

-

A

V

L

L

S

G

Y

Y

I

G

L

V

I

A

G

G

L

I

M

I

V

A

Y

F

F

L

L

I

M

M

T

R

S

Q

L

L

G

G

E

E

L

M

A

V

F

E

M

E

P

P

V

V

S

S

G

G

S

S

F
|
F

A

A

T

H

Y
x
F

G

A

Q
x
Y

N

K

Y
|
Y

V
x
W

E

G

E

P

G
x
F

F

A

G

G

F
|
F

A

L

L

S

G

G

W
|
W

N

N

Y
|
Y

W
|
W

Y

V

N

M

W
x
F

A

V

V

L

T

V

I

G

A

M

V

A

D
x
E

L

L

V

T

A

A

S

A

Q

G

L

I

V

Y

M

M

S

Q

Y

Y

W

W

F

F

P

P

D

D

T

V

P

P

G

T

W

W

I

I

W

W

S

A

A

A

L

A

F

F

L

F

G

I

I

I

M

I

F

N

L

A

L

V

N

N

W

L

I

V

S

N

V

V

R

R

G

L

F

Y

G

G

E

E

A

T

E

E

Y

F

W

W

F

F

S

A

L

L

I

I

K
|
K

V

V

A

L

T

A

V

I

I

I

I

G

F

M

I

I

I

G

V

F

G

G

V

L

M

W

M

L

I

L

V

F

G

S

I

G

F

H

K

G

G

G

S

E

Q

K

P

A

T

S

G

I

W

D

S

N

N

L

W

W

G

R

I

Y

A

G

D

G

A

F

P

-

F

F

A

A

G

T

G

G

F

W

S

N

A

G

M

L

I

I

G

L

V

S

A

L

M

A

I

V

V

I

G

M

F

F

S

S

F

F

Q

G

G

G

T

L

E
|
E

L

L

I

I

G

G

I

I

A

T

A

A

G

A

E

E

S

A

E

R

D

D

P

P

E

E

K

K

N

S

I

I

P

P

R

K

A

A

V

V

R

N

Q

Q

V

V

F

V

W

Y

R
|
R

I

I

L

L

F

F

Y

Y

V

I

F

G

A

S

I

L

L

V

I

V

I

L

S

L

L

A

I

L

I

Y

P

P

Y

W

T

V

D

-

P

-

S

-

L

-

R

-

N

-

D

E

V

V

K

K

D

S

I

N

S

S

V

-

S

S

P

P

F

F

T

V

L

M

V

I

F

F

Q

H

H

N

A

L

G

D

L

S

L

N

S

V

A

V

A

A

A

S

I

A

M

L

N

N

A

F

V

V

I

I

L

L

T

V

A

A

V

S

L

L

S

S

A

V

G

Y

N

N

S

S

G

G

M

V

Y

Y

A

S

S

N

T

S

R

R

M

M

L

L

Y

F

T

G

L

L

A

S

C

V

D

Q

G

G

K

N

A

A

P

P

R

K

I

F

F

L

S

T

K

R

L

V

S

S

R

R

G

R

G

G

V

V

P
|
P

R

I

N

N

A

S

L

L

Y

M

A

L

T

S

T

G

V

A

I

I

A

T

A

S

L

L

C

V

F

V

L

L

T

I

S

N

M

Y

F

L

G

L

N

P

Q

Q

T

K

V

A

Y

F

L

G

W

L

L

L

L

M

N

A

T

L

S

V

G

V

M

A

T

T

G

L

F

L

I

L

A

N

W

W

L

I

G

M

I

I

A

C

I

L

S

A

H

H

Y

L

R

R

F

F

R

R

R

A

G

A

Y

M

V

R

M

R

Q

Q

G

G

N

R

D

E

I

T

N

Q

N

-

L

-

P

-

Y

F

R

K

S

A

G

L

F

L

F

Y

P

P

L

F

G

G

R26 (= R20) mutation R->G,S,Q: Strong decrease in phenylalanine transport activity.
P54 (= P48) mutation to A: 50% of wild-type phenylalanine transport activity.; mutation to G: No change in phenylalanine transport activity.; mutation to L: 26% of wild-type phenylalanine transport activity.
F87 (= F82) mutation to L: No effect on phenylalanine transport activity.
F90 (≠ Y85) mutation to L: 65% of wild-type phenylalanine transport activity.
Y92 (≠ Q87) mutation to L: 41% of wild-type phenylalanine transport activity.
Y94 (= Y89) mutation to L: 69% of wild-type phenylalanine transport activity.
W95 (≠ V90) mutation to L: 10% of wild-type phenylalanine transport activity.
F98 (≠ G93) mutation to L: No effect on phenylalanine transport activity.
F101 (= F96) mutation to L: 38% of wild-type phenylalanine transport activity.
W105 (= W100) mutation to L: 39% of wild-type phenylalanine transport activity.
Y107 (= Y102) mutation to L: No effect on phenylalanine transport activity.
W108 (= W103) mutation to L: 71% of wild-type phenylalanine transport activity.
F111 (≠ W106) mutation to L: 60% of wild-type phenylalanine transport activity.; mutation to Y: Enables the transport of tryptophan to almost the same steady-state level as that of phenylalanine.
E118 (≠ D113) mutation E->G,L,V,N: Loss of activity.
K168 (= K163) mutation K->L,R: Strong decrease in phenylalanine transport activity.; mutation to N: Loss of activity.
E226 (= E222) mutation E->A,Q,K,R,W: Loss of activity.
R252 (= R248) mutation R->D,E,F,W,P: Loss of activity.
P341 (= P345) mutation to A: 5% of wild-type phenylalanine transport activity.; mutation P->G,Q,K,R: Loss of activity.; mutation to S: 3% of wild-type phenylalanine transport activity.; mutation to T: 17% of wild-type phenylalanine transport activity.

Sites not aligning to the query:

442 P→A: 46% of wild-type phenylalanine transport activity.; P→G: 52% of wild-type phenylalanine transport activity.; P→L: 43% of wild-type phenylalanine transport activity.

P15993 Aromatic amino acid transport protein AroP; Aromatic amino acid:H(+) symporter AroP; General aromatic amino acid permease; General aromatic transport system from Escherichia coli (strain K12) (see paper)
35% identity, 84% coverage: 9:420/489 of query aligns to 7:405/457 of P15993

query
sites
P15993

E

H

A

G

P

E

T

Q

L

L

R

K

R

R

E

G

L

L

K

K

A

N

R

R

H

H

L

I

T

Q

M

L

I

I

A

A

I

L

G

G

S

A

I

I

G

G

T

T

G

G

L

L

F

F

V

L

A

G

S

S

G

A

A

S

T

V

I

I

S

Q

Q

S

A

A

G

G

P

P

G

G

G

-

A

I

L

I

L

L

S

G

Y

Y

I

A

L

I

I

A

G

G

L

F

M

I

V

A

Y

F

F

L

L

I

M

M

T

R

S

Q

L

L

G

G

E

E

L

M

A

V

F

E

M

E

P

P

V

V

S

A

G

G

S

S

F

F

A

S

T

H

Y

F

G

A

Q

Y

N

K

Y

Y

V

W

E

G

E

S

G

F

F

A

G

G

F

F

A

A

L

S

G

G

W

W

N

N

Y

Y

W

W

Y

V

N

L

W
x
Y

A

V

V

L

T

V

I

A

A

M

V

A

D

E

L

L

V

T

A

A

S

V

Q

G

L

K

V

Y

M

I

S

Q

Y

F

W

W

F

Y

P

P

D

E

T

I

P

P

G

T

W

W

I

V

W

S

S

A

A

A

L

V

F

F

L

F

G

V

I

V

M

I

F

N

L

A

L

I

N

N

W

L

I

T

S

N

V

V

R

K

G

V

F

F

G

G

E

E

A

M

E

E

Y

F

W

W

F

F

S

A

L

I

I

I

K

K

V

V

A

I

T

A

V

V

I

V

I

A

F

M

I

I

V

F

G

G

V

G

M

W

M

L

I

L

V

F

G

S

I

G

F

N

K

G

G

G

S

P

Q

Q

P

A

T

T

G

V

W

S

S

N

N

L

W

W

G

D

I

-

A

Q

D

G

A

G

P

F

F

L

A

P

G

H

G

G

F

F

S

T

A

G

M

L

I

V

G

M

V

M

A

M

M

A

I

I

V

I

G

M

F

F

S

S

F

F

Q

G

G

G

T

L

E

E

L

L

I

V

G

G

I

I

A

T

A

A

G

A

E

E

S

A

E

D

D

N

P

P

E

E

K

Q

N

S

I

I

P

P

R

K

A

A

V

T

R

N

Q

Q

V

V

F

I

W

Y

R

R

I

I

L

L

L

I

F

F

Y

Y

V

I

F

G

A

S

I

L

L

A

I

V

I

L

S

L

L

S

I

L

I

M

P

P

Y

W

T

T

D

R

P

V

S

T

L

-

R

-

N

-

D

-

V

-

K

-

D

-

I

A

S

D

V

T

S

S

P

P

F

F

T

V

L

L

V

I

F

F

Q

H

H

E

A

L

G

G

L

D

L

T

S

F

A

V

A

A

A

N

I

A

M

L

N

N

A

I

V

V

I

V

L

L

T

T

A

A

V

A

L

L

S

S

A

V

G

Y

N

N

S

S

G

C

M

V

Y

Y

A

C

S

N

T

S

R

R

M

M

L

L

Y

F

T

G

L

L

A

A

C

Q

D

Q

G

G

K

N

A

A

P

P

R

K

I

A

F

L

S

A

K

S

L

V

S

D

R

K

G

R

G

G

V

V

P

P

R

V

N

N

A

T

L

I

Y

L

A

V

T

S

T

A

V

L

I

V

A

T

A

A

L

L

C

C

F

V

L

L

T

I

S

N

M

Y

F

L

G

A

N

P

Q

E

T

S

V

A

Y

F

L

G

W

L

L

L

L

M

N

A

T

L

S

V

G

V

M

S

T

A

G

L

F

V

I

I

A

N

W

W

L

A

G

M

I

I

A

S

I

L

S

A

H

H

Y

M

R

K

F

F

R

R

G

A

Y

K

V

Q

M

E

Q

Q

G

G

N

-

D

V

I

V

N

T

N

R

L

F

P

P

Y

-

R

-

S

A

G

L

F

L

F

Y

P

P

L

L

G

G

Y103 (≠ W106) Key residue for tryptophan transport; mutation to F: Decreases tryptophan transport to less than 50% of wild-type levels and reduces the ability of tryptophan to inhibit phenylalanine transport from 95 to 62%.

Q88CZ8 L-histidine transporter HutT from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see paper)
36% identity, 87% coverage: 8:431/489 of query aligns to 2:417/467 of Q88CZ8

query
sites
Q88CZ8

T

Q

E

Q

A

A

P

Q

T

G

L

L

R

K

R

R

E

G

L

L

K

S

A

A

R

R

H

H

L

I

T

R

M

F

I

M

A

A

I

L

G

G

G

S

S

A

I

I

G

G

T
|
T

G

G

L

L

F

F

V

Y

A

G

S

S

G

A

A

S

T

A

I

I

S

Q

Q

M

A

A

G

G

P

P

G

A

G

-

A

V

L

L

S

A

Y

Y

I

L

L

I

I

G

G

G

L

A

M

A

V

V

Y

F

F

M

L

V

M

M

T

R

S

A

L

L

G

G

E

E

L

M

A

A

A

V

F

H

M

N

P

P

V

V

S

A

G

G

S

S

F

F

A

G

T

H

Y

Y

G

A

Q

T

N

T

Y

Y

V

L

E

G

E

P

G

M

F

A

G

G

F

F

A

I

L

L

G

G

W

W

N

T

Y

Y

W

A

Y

F

N
x
E

W

M

A

V

V

I

T

V

I

A

A

I

V

A

D

D

L

V

V

T

A

A

S

F

Q

G

L

I

V

Y

M

M

S

G

Y

F

W

W

F

F

P

P

D

E

T

V

P

A

G

R

W

W

I

I

W

W

S

-

A

-

L

-

F

V

L

L

G

G

I

I

M

V

F

F

L

L

-

I

-

G

L

L

N

N

W

L

I

C

S

N

V

V

R

K

G

V

F

F

G

G

E

E

A

M

E

E

Y

F

W

W

F

L

S

S

L

L

I

L

K
|
K

V

V

A

G

T

A

V

I

I

V

I

A

F

M

I

I

I

L

V

A

G

G

V

L

M

G

M

I

I

M

V

A

G

-

I

-

F

F

K

G

G

F

S

S

Q

Q

P

V

T

G

G

T

W

G

S

H

N

A

W

V

G

G

I

M

A

S

D

N

A

L

P

F

F

D

A

H

G

G

F

F

-

M

-

P

-

N

-

G

-

V

S

G

A

G

M

L

I

I

G

A

V

S

A

F

M

A

I

V

V

V

G

M

F
|
F

S

A

F

F

Q

G

G

G

T

I

E
|
E

L

I

I

I

G

G

I

V

A

T

A

A

G

G

E

E

S

A

E

K

D

D

P

P

E

Q

K

R

N

V

I

I

P

P

R

K

A

A

V

I

R

N

Q

A

V

V

F

P

W

L

R

R

I

I

L

L

F

F

Y

Y

V

V

F

L

A

T

I

L

L

F

I

V

I

L

S

M

L

C

I

L

I

Y

P

P

Y

W

T

-

D

-

P

P

S

Q

L

I

L

G

R

S

N

Q

D

G

V

-

K

-

D

-

I

-

S

-

V

-

S

S

P

P

F

F

T

V

L

Q

V

I

F

F

Q

S

H

N

A

L

G

G

L

I

L

G

S

S

A

A

I

V

M

L

N

N

A

V

V

V

I

V

L

I

T

S

A

A

V

A

L

I

S

S

A

A

G

I

N

N

S

S

G

D

M

I

Y

F

A

G

S

A

T

G

R

R

M

M

L

M

Y

Y

T

G

L

L

A

A

C

Q

D

Q

G

G

K

H

A

A

P

P

R

R

I

G

F

F

S

S

K

K

L

L

S

S

R

K

G

H

G

G

V

V

P

P

R

-

N

-

A

-

L

-

Y

W

A

M

T

T

T

V

V

V

I

V

A

M

A

G

L

A

C

A

F

L

L

L

T

I

S

G

M

V

F

L

G

L

N

N

-

Y

-

L

-

I

-

P

Q

E

T

N

V

V

Y

F

L

L

W

L

L

I

L

A

N

S

T

I

S

A

G

T

M

F

T

A

G

T

F

V

I

W

A

V

W

W

L

L

G

M

I

I

A

L

I

L

S

T

H

Q

Y

V

R

A

F

M

R

R

G

S

Y

-

V

-

M

M

Q

S

G

R

N

E

D

Q

I

V

N

A

N

Q

L

L

P

K

Y

F

R

P

S

V

G

P

F

F

F

W

P

P

L

Y

G

G

P

P

I

A

F

M

A

A

F

I

V

A

L

F

C

M

L

V

I

F

I

I

T27 (= T33) mutation T->A,S: Retains 60% of wild-type activity.; mutation to N: Retains 20% of wild-type activity.
E98 (≠ N105) mutation to A: Retains 80% of wild-type activity.
K156 (= K163) mutation K->A,Q: Retains less than 10% of wild-type activity.; mutation to R: Retains 40% of wild-type activity.
F212 (= F216) mutation F->A,Q: Loss of activity.; mutation to Y: No change in activity.
E218 (= E222) mutation E->A,Q: Loss of activity.; mutation to D: Retains 70% of wild-type activity.

P48813 High-affinity glutamine permease from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
27% identity, 96% coverage: 4:471/489 of query aligns to 136:612/663 of P48813

query
sites
P48813

E

Q

T

E

K

Q

T

E

T

Q

E

K

A

Q

P

E

T

N

L

L

R

K

E

S

L

I

K

K

A

P

R

R

H

H

L

T

T

V

M

M

I

M

A

S

I

L

G

G

G

T

S

G

I

I

G

G

T

T

G

G

L

L

F

L

V

V

A

G

S

N

G

S

A

K

T

V

I

L

S

N

Q

N

A

A

G

G

P

P

G

G

A

L

L

I

S

G

Y

Y

I

A

L

I

I

M

G

G

L

S

M

C

V

V

Y

Y

F

C

L

I

M

I

T

Q

S

A

L

C

G

G

E

E

L

L

A

A

A

V

-

I

F

Y

M

S

P

D

V

L

S

I

G

G

S

G

F

F

A

N

T

T

Y

Y

G

P

Q

L

N

F

Y

L

V

V

E

D

E

P

G

A

F

L

G

G

F

F

A

S

L

V

G

A

W

W

N

L

Y

F

W

C

Y

L

N

Q

W

W

A

L

V

C

T

V

I

C

A

P

V

L

D

E

L

L

V

V

A

T

S

A

Q

S

L

M

V

T

M

I

S

K

Y

Y

W

W

F

T

P

T

D

S

T

V

P

N

G

P

W

D

I

V

W

F

S

V

A

V

L

I

F

F

L

Y

G

V

I

L

M

I

F

V

L

V

L

I

N

N

W

V

I

F

S

G

V

A

R

K

G

G

F

Y

G

A

E

E

A

A

E

D

Y

F

W

F

F

F

S

N

L

C

I

C

K

K

V

I

A

L

T

M

V

I

I

V

I

G

F

F

I

F

I

I

V

L

G

A

V

I

M

I

I

D

V

C

G

G

I

-

F

-

K

-

G

G

S

A

Q

G

P

T

T

D

G

G

-

Y

-

I

-

G

-

S

-

K

-

Y

W

W

S

R

N

D

W

P

G

G

I

A

-

F

-

R

A

G

D

D

A

T

P

P

F

I

A

Q

G

R

G

-

F

F

S

K

A

G

M

V

I

V

G

A

V

T

A

F

M

V

I

T

V

A

G

A

F

F

S

A

F

F

Q

G

G

M

T

S

E

E

L

Q

I

L

G

A

I

M

A

T

A

A

G

S

E

E

S

Q

E

S

D

N

P

P

E

R

K

K

N

A

I

I

P

P

R

S

A

A

V

A

R

K

Q

K

V

M

F

I

W

Y

R

R

I

I

L

L

L

F

F

V

Y

F

V

L

F

A

A

S

I

L

L

T

I

L

I

V

S

G

L

F

I

L

I

V

P

P

Y

Y

T

T

D

S

P

D

S

Q

L

L

R

G

N

A

D

A

V

G

K

S

D

A

I

T

S

K

V

A

S

S

P

P

F

Y

T

V

L

I

V

A

F

V

Q

S

H

S

A

H

G

G

L

V

L

R

S

V

A

V

A

P

A

H

I

F

M

I

N

N

A

A

V

V

I

I

L

L

T

L

A

S

V

V

L

L

S

S

A

V

G

A

N

N

S

G

G

A

M

F

Y

Y

A

T

S

S

T

S

R

R

M

I

L

L

Y

M

T

S

L

L

A

A

C

K

D

Q

G

G

K

N

A

A

P

P

R

K

I

C

F

F

S

D

K

Y

L

I

S

D

R

R

G

E

G

G

V

R

P

P

R

A

N

A

A

A

L

M

Y

L

A

V

T

S

T

A

V

L

I

F

A

G

A

V

L

I

C

A

F

F

L

C

T

A

S

S

M

S

F

K

G

K

N

E

Q

E

T

D

V

V

Y

F

L

T

W

W

L

L

N

A

T

I

S

S

G

G

M

L

T

S

G

Q

F

L

I

F

A

T

W

W

L

I

G

T

I

I

A

C

I

L

S

S

H

H

Y

I

R

R

F

F

R

R

G

A

Y

M

V

K

M

V

Q

Q

G

G

N

R

D

S

I

L

N

G

N

E

L

V

P

G

Y

Y

R

K

S

S

G

Q

F

V

F

G

P

V

L

W

G

G

P

S

I

A

F

Y

A

A

F

V

V

L

L

M

C

M

L

V

I

L

I

A

T

L

L

I

G

A

Q

Q

N

F

Y

W

E

V

A

A

F

I

L

A

K

-

D

-

T

P

I

I

D

G

W

G

G

G

V

K

A

L

A

S

-

A

-

Q

-

S

-

F

-

E

T

N

Y

Y

I

L

G

A

I

M

P

P

L

I

F

W

L

I

V

A

I

L

W

Y

F

I

G

F

Y

Y

K

K

L

V

A

W

K

K

Sites not aligning to the query:

132 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Q03770 SPS-sensor component SSY1; Amino-acid permease homolog SSY1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
27% identity, 98% coverage: 3:481/489 of query aligns to 267:816/852 of Q03770

query
sites
Q03770

S

N

E

R

T

S

K

R

T

A

T

T

E

R

A

K

P

Y

T

H

L

I

R

Q

R

R

E

K

L

L

K

K

A

V

R

R

H

H

L

I

T

Q

M

M

I

L

A

S

I

I

G

G

G

A

S

C

I

F

G

S

T

V

G

G

L

L

F

F

V

L

A

T

S

S

G

G

A

K

T

A

I

F

S

S

Q

I

A

A

G

G

P

P

G

F

G

G

A

T

L

L

S

G

Y

F

I

G

L

L

I

T

G

G

L

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M

I

V

I

Y

L

F

A

L

T

M

M

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L

S

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L

F

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E

E

L

L

A

S

A

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L

M

I

P

P

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V

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S

G

S

S

G

F

F

A

S

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Y

L

G

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V

E

E

E

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A

F

F

G

G

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F

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A

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L

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G

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W

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Y

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W

Y

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x
T

L

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M

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S

Y

Y

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Y

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N

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V

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L

L

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V

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L

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N

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D

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F

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D

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W

T

G

K

G

A

V

F

A

F

A

S

T

A

Y

Y

I

G

G

G

I

L

P

M

L

F

F

F

L

F

V

I

I

S

W

Y

F

T

G

A

Y

Y

K

K

L

V

A

L

K

G

G

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T

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K

F

I

V

Q

R

R

Y

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S

D

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M

L

T382 (≠ Q118) mutation T->H,L: Constitutively active, up-regulates amino acid permease transcription in response to subthreshold concentrations of amino acids.; mutation to K: In SSY1-102; constitutively active, up-regulates amino acid permease transcription in the absence of amino-acids.; mutation to R: Constitutively active, up-regulates amino acid permease transcription in the absence of amino acids.

6f34A Crystal structure of a bacterial cationic amino acid transporter (cat) homologue bound to arginine. (see paper)
29% identity, 85% coverage: 12:428/489 of query aligns to 22:421/458 of 6f34A

query
sites
6f34A

T

S

L

L

R

R

R

K

E

E

L

L

K
x
G

A

A

R
x
F

H
x
D

L

L

T

T

M
|
M

I

L

A

G

I

I

G

G

G

A

S
x
I

I

I

G
|
G

T
|
T

G
|
G

L

I

F

F

V

V

A

L

S

T

G

G

-

V

A

A

T

A

I

A

S

E

Q

H

A

A

G

G

P

P

G

A

G

-

A

L

L

V

L

L

S

S

Y

F

I

I

L

L

I

S

G

G

L

L

M

A

V

C

Y

V

F

F

L

A

M

A

T

L

S

C

L

Y

G

A

E

E

L

F

A

A

A

S

F

T

M

V

P

P

V

V

S

S

G

G

S

S

F

A

A

Y

T

T

Y

Y

G

S

Q

Y

N

A

Y

T

V

F

E

G

E

E

G

L

F

I

G

A

F

W

A

I

L

L

G

G

W

W

N

D

Y

L

W

I

Y

L

N
x
E

W
x
Y

A

G

V

V

-
x
A

-

S

T

A

I

V

A

A

V

V

-

G

-

W

-

S

-

G

-

Y

-

F

-

Q

D

G

L

L

V

L

A

S

S

G

Q

E

L

L

-

P

-

K

-

A

V

L

M

T

S

S

Y

A

W

Y

F

D

P

P

-

A

-

K

-

G

-

T

-

F

-

I

D

D

T

L

P

P

G

A

W

I

I

I

W

-

S

-

A

-

L

I

F

V

L

L

G

F

I

I

M

T

F

F

L

L

N

N

W

-

I

L

S

G

V

A

R

K

G

K

F

S

G
x
A

E
x
R

A

F

E

N

Y

A

W

V

F

I

S

V

L
x
A

I
|
I

K

K

V

V

A
|
A

T

V

V

V

I

L

I
x
L

F

F

I

L

I

A

V

V

G

G

V

V

M
x
W

M
x
Y

I

V

V

-

G

-

I

-

F

-

K

-

G

-

S

-

Q

K

P

P

T

E

G

N

W

W

S

T

N

P

W

F

G

-

I

-

A

-

D

-

A

M

P

P

F

Y

A

-

G

-

G

G

F

F

S

S

A

G

M

V

I

A

G

T

V

G

A

A

M

A

I

T

V

V

G

F

F
|
F

S
x
A

F

Y

Q
x
I

G

G

T

F

E

D

L

A

I

V

G

S

I

T

A

A

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E

E

E

S

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P

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K

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Y

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L

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I

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P

Y

Y

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D

Q

P

L

S

N

L

-

L

V

R

K

N

N

D

P

V

V

K

A

D

-

I

-

S

-

V

-

S

-

P

-

F

F

T

A

L

L

V

N

F

Y

Q

I

H

H

-
x
Q

-

D

-

W

-
x
V

A

A

G

G

L

F

L

I

S

S

A

L

A

G

A

A

I

I

M

-

N

-

A

A

V

G

I

I

L

T

T

T

A
x
V

V

L

L

L

S

V

A

S

G

-

N

-

S

-

G

-

M

M

Y

Y

A

G

S

Q

T

T

R

R

M

L

L

F

Y

Y

T

A

L

I

A

S

C

R

D

D

G

G

K

L

A

L

P

P

R

K

I

V

F

F

S

A

K

R

L

I

S

S

R

P

G

T

G

-

V

-

P

-

R

R

N

Q

A

V

L

P

Y

Y

A

V

T

N

T

T

V

W

I

L

A

T

A

G

L

A

C

A

F

-

L

-

T

V

S

A

M

V

F

F

G

A

N

G

Q

I

T

I

V

P

Y

L

L

N

W

K

L

L

L

A

N

E

T

L

S

T

G

N

M

I

T

G

G

T

F

L

I

F

A

A

W

F

L

I

G

T

I

V

A

S

I

I

S

G

H

V

Y

L

R

V

F

L

R

R

R

K

G

-

Y

-

V

-

M

-

Q

T

G

Q

N

P

D

D

I

L

N

K

N

R

L

-

P

A

Y

F

R

R

S

V

G

P

F

F

F

V

P

P

L

V

G

V

P

P

I

I

F

L

A

A

F

V

V

L

L

F

C

C

binding arginine: I40 (≠ S30), G42 (= G32), T43 (= T33), G44 (= G34), E115 (≠ N105), Y116 (≠ W106), A119 (vs. gap), F228 (= F216), A229 (≠ S217), I231 (≠ Q219), V314 (≠ A307)
binding cholesterol: W201 (≠ M176), Y202 (≠ M177)
binding : G28 (≠ K18), F30 (≠ R20), D31 (≠ H21), M34 (= M24), A178 (≠ G153), R179 (≠ E154), A186 (≠ L161), I187 (= I162), A190 (= A165), L194 (≠ I169), Q296 (vs. gap), V299 (vs. gap)

5oqtA Crystal structure of a bacterial cationic amino acid transporter (cat) homologue (see paper)
28% identity, 85% coverage: 12:428/489 of query aligns to 20:419/456 of 5oqtA

query
sites
5oqtA

T

S

L

L

R

R

R

K

E
|
E

L

L

K
x
G

A

A

R
x
F

H
x
D

L

L

T

T

M
|
M

I

L

A

G

I

I

G

G

G

A

S
x
I

I

I

G
|
G

T
|
T

G
|
G

L

I

F

F

V

V

A

L

S

T

G

G

-

V

A

A

T

A

I

A

S

E

Q

H

A

A

G

G

P

P

G

A

G

-

A

L

L

V

L

L

S

S

Y

F

I

I

L

L

I

S

G

G

L

L

M

A

V

C

Y

V

F

F

L

A

M

A

T

L

S

C

L

Y

G

A

E

E

L

F

A

A

A

S

F

T

M

V

P

P

V

V

S

S

G

G

S

S

F

A

A

Y

T

T

Y

Y

G

S

Q

Y

N

A

Y

T

V

F

E

G

E

E

G

L

F

I

G

A

F

W

A

I

L

L

G

G

W

W

N

D

Y

L

W

I

Y

L

N

E

W

Y

A

G

V

V

-

A

-

S

T

A

I

V

A

A

V

V

-

G

-

W

-

S

-

G

-

Y

-

F

-

Q

D

G

L

L

V

L

A

S

S

G

Q

E

L

L

-

P

-

K

-

A

V

L

M

T

S

S

Y

A

W

Y

F

D

P

P

-

A

-

K

-

G

-

T

-

F

-

I

D

D

T

L

P

P

G

A

W

I

I

I

W

-

S

-

A

-

L

I

F

V

L

L

G

F

I

I

M

T

F

F

L

L

N

N

W

-

I

L

S

G

V

A

R

K

G

K

F

S

G
x
A

E
x
R

A

F

E

N

Y

A

W

V

F

I

S

V

L
x
A

I

I

K

K

V

V

A
|
A

T

V

V

V

I

L

I
x
L

F

F

I

L

I

A

V

V

G

G

V

V

M

W

M

Y

I

V

V

-

G

-

I

-

F

-

K

-

G

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S

-

Q

K

P

P

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E

G

N

W

W

S

T

N

P

W

F

G

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I

-

A

-

D

-

A

M

P

P

F

Y

A

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G

-

G

G

F

F

S

S

A

G

M

V

I

A

G

T

V

G

A

A

M

A

I

T

V

V

G

F

F
|
F

S
x
A

F

Y

Q
x
I

G

G

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F

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D

L

A

I

V

G

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A

A

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E

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L

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Y

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L

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L

V

R

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N

N

D

P

V

V

K

A

D

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I

-

S

-

V

-

S

-

P

-

F

F

T

A

L

L

V

N

F

Y

Q

I

H

H

-
x
Q

-

D

-

W

-
x
V

A

A

G

G

L

F

L

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S

S

A

L

A

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A

A

I

I

M

A

N

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A

I

V

T

I

T

L

V

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L

A

L

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V

L

M

S

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A

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N

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M

M

Y

Y

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L

L

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D

D

G

G

K

L

A

L

P

P

R

K

I

V

F

F

S

A

K

R

L

I

S

S

R

P

G

T

G

-

V

-

P

-

R

R

N

Q

A

V

L

P

Y

Y

A

V

T

N

T

T

V

W

I

L

A

T

A

G

L

A

C

A

F

-

L

-

T

V

S

A

M

V

F

F

G

A

N

G

Q

I

T

I

V

P

Y

L

L

N

W

K

L

L

L

A

N

E

T

L

S

T

G

N

M

I

T

G

G

T

F

L

I

F

A

A

W

F

L

I

G

T

I

V

A

S

I

I

S

G

H

V

Y

L

R

V

F

L

R

R

R

K

G

-

Y

-

V

-

M

-

Q

T

G

Q

N

P

D

D

I

L

N

K

N

R

L

-

P

A

Y

F

R

R

S

V

G

P

F

F

F

V

P

P

L

V

G

V

P

P

I

I

F

L

A

A

F

V

V

L

L

F

C

C

binding alanine: I38 (≠ S30), G40 (= G32), T41 (= T33), G42 (= G34), F226 (= F216), A227 (≠ S217), I229 (≠ Q219)
binding : E24 (= E16), G26 (≠ K18), F28 (≠ R20), D29 (≠ H21), M32 (= M24), A176 (≠ G153), R177 (≠ E154), A184 (≠ L161), A188 (= A165), L192 (≠ I169), Q294 (vs. gap), V297 (vs. gap)

P76037 Putrescine importer PuuP from Escherichia coli (strain K12) (see paper)
27% identity, 47% coverage: 13:242/489 of query aligns to 18:242/461 of P76037

query
sites
P76037

L

L

R

R

R

K

E

S

L

L

K

K

A

L

R

W

H

Q

L

V

T

V

M

M

I

M

A

G

I

L

G

A

G

Y

S

L

I

T

G

P

T

M

G

T

L

V

F

F

V

D

A

T

S

F

G

G

A

-

T

I

I

V

S

S

Q

G

A

I

G

S

P

D

G

G

G

H

A

V

L

P

L

A

S

S

Y

Y

I

L

L

L

I

A

G

L

L

A

M

G

V

V

Y

L

F

F

L

T

M

A

T

I

S

S

L

Y

G

G

E

K

L

L

A

V

A

R

F

Q

M

F

P

P

V

E

S

A

G

G

S

S

F

A

A

Y

T

T

Y

Y

G

A

Q

Q

N

K

Y

S

V

I

E

N

E

P

G

H

F

V

G

G

F

F

A

M

L

V

G

G

W

W

N

S

Y

S

W

L

Y

L

N

D

W
x
Y

A

L

V

F

T

L

I

P

A

M

V

I

D

N

L

V

V

L

A

L

S

A

Q

K

L

I

V

Y

M

L

S

S

Y

A

W

L

F

F

P

P

D

E

T

V

P

P

G

P

W

W

I

V

W

W

S

V

A

V

L

T

F

F

L

V

G

A

I

I

M

L

F

T

L

A

N

N

W

L

I

K

S

S

V

V

R

N

G

L

F

V

G

A

E

N

A

F

E

N

Y

T

W

L

F

F

S

V

L

L

I

V

K

Q

V

I

A

S

T

I

V

M

I

V

F

F

I

I

I

F

V

-

G

-

V

-

M

L

M

V

I

V

V

Q

G

G

I

L

F

H

K

K

G

G

S

E

Q

G

P

V

-

G

T

T

G

V

W

W

S

S

N

-

W

-

G

-

I

-

A

-

D

L

A

Q

P

P

F

F

A

I

G

S

G

E

F

N

S

A

A

H

M

L

I

I

G

P

V

I

-

I

-

T

-

G

A

A

M

T

I

I

V

V

G

C

F

F

S

S

F

F

Q

L

G

G

T

F

E

D

L

A

I

V

G

T

I

T

A

L

A

S

G

E

E

E

S

T

E

P

D

D

P

A

E

A

K

R

N

V

I

I

P

P

R

K

A

A

V

I

Y110 (≠ W106) mutation to X: The uptake activity is reduced to one-eighth of that of wild-type.

P30825 High affinity cationic amino acid transporter 1; CAT-1; CAT1; Ecotropic retroviral leukemia receptor homolog; Ecotropic retrovirus receptor homolog; Solute carrier family 7 member 1; System Y+ basic amino acid transporter from Homo sapiens (Human) (see paper)
21% identity, 72% coverage: 7:358/489 of query aligns to 22:398/629 of P30825

query
sites
P30825

T

S

T

R

E

E

A

E

P

T

T

R

L

L

R

S

R

R

E

C

L

L

K

N

A

T

R

F

H

D

L

L

T

V

M

A

I

L

A

G

I

V

G

G

G

S

S

T

I

L

G

G

T

A

G

G

L

V

F

Y

V

V

A

L

S

A

G

G

A

A

T

V

I

A

S

R

Q

E

A

N

G

A

P

G

G

P

G

A

A

I

L

V

L

I

S

S

Y

F

I

L

L

I

I

A

G

A

L

L

M

A

V

S

Y

V

F

L

L

A

M

G

T

L

S

C

L

Y

G

G

E

E

L

F

A

G

A

A

F

R

M

V

P

P

V

K

S

T

G

G

S

S

F

A

A

Y

T

L

Y

Y

G

S

Q

Y

N

V

Y

T

V

V

E

G

E

E

G

L

F

W

G

A

F

F

A

I

L

T

G

G

W

W

N

N

Y

L

W

I

Y

L

N

S

-

Y

-

I

-

G

-

T

-

S

-

V

-

A

-

R

-

A

W

W

A

S

V

A

T

T

I

F

A

D

V

-

D

E

L

L

V

I

A

G

S

R

-

P

-

I

-

G

Q

E

L

F

V

S

M

R

S

T

Y

H

W

M

F

T

P

L

D

N

T

A

P

P

G

G

W

V

I

L

W

A

S

E

A

N

L

P

F

D

L

I

G

F

I

A

M

V

F

I

L

I

N

L

W

I

I

L

S

T

V

G

R

L

G

L

F

T

G

L

E

G

A

V

E

K

Y

E

W

S

F

A

S

M

L

V

I

N

K

K

V

I

A

F

T

T

V

C

I

I

I

N

F

V

I

L

I

V

V

L

G

G

V

F

M

I

M

M

I

V

V

S

G

G

I

F

F

V

K

K

G

G

S

S

Q

V

P

-

T

-

G

-

W

-

S

K

N

N

W

W

G

Q

I

L

A

T

D

E

A

E

P

D

F

F

A

G

G
x
N

-

T

-

S

-

G

-

R

-

L

-

C

-

L

-

N

-

D

-

T

-

K

-

E

-

G

-

K

-

P

-

G

-

V

-

G

-

F

-

M

-

P

-

F

G

G

F

F

S

S

A

G

M

V

I

L

G

S

V

G

A

A

M

A

I

T

V

C

G

F

F

Y

S

A

F

F

Q

V

G

G

T

F

E

D

L

C

I

I

G

A

I

T

A

T

A

G

G

E

E

E

S

V

E

K

D

N

P

P

E

Q

K

K

N

A

I

I

P

P

R

V

A

G

V

I

R

V

Q

A

V

S

F

L

W

L

R

I

I

C

L

F

L

I

F

A

Y

Y

V

F

F

G

A

V

I

S

L

A

I

A

I

L

S

T

L

L

I

M

P

P

Y

Y

T

F

D

-

P

-

S

-

L

C

L

L

R

D

N

N

D

N

V

-

K

-

D

-

I

-

S

-

V

-

S

S

P

P

F

L

T

P

L

D

V

A

F

F

Q

K

H

H

A

V

G

G

L

W

L

E

S

G

A

A

A

K

A

Y

I

A

M

V

N

A

A

V

V

G

I

S

L

L

T

C

A

A

V

L

L

S

S

A

A

S

G

L

N

L

S

G

G

S

M

M

Y

F

A

P

S

M

T

P

R

R

M

V

L

I

Y

Y

T

A

L

M

A

A

C

E

D

D

G

G

K

L

A

L

P

F

R

K

I

F

F

L

S

A

K

N

L

V

S

N

-

D

R

R

G

T

G

K

V

T

P

P

R

I

N

I

A

A

L

T

Y

L

A

A

T

S

T

G

V

A

I

V

A

A

L

V

N226 (≠ G202) modified: carbohydrate, N-linked (GlcNAc...) asparagine

6f2wA Bacterial asc transporter crystal structure in open to in conformation (see paper)
22% identity, 91% coverage: 15:460/489 of query aligns to 2:424/433 of 6f2wA

query
sites
6f2wA

R

K

E

E

L

V

K

S

A

G

R

I

H

T

L

A

T

L

M

T

I

V

A

V

I

V

G

G

G

T

S
x
V

I

I

G
|
G

T
x
A

G
|
G

L

I

F

F

V

F

A

K

S

P

G

T

A

A

T

V

I

Y

S

G

Q

A

A

A

G

G

-

A

P

P

G

G

G

L

A

G

L

L

S

A

Y

W

I

F

L

V

I

A

G

G

L

I

M

I

V

T

Y

I

F

A

L

G

M

G

T

L

S

T

L

V

G

A

E

E

L

I

A

G

A

T

F

I

M

Y

P

P

V

Q

S

T

G

G

S

G

F

M

A

M

T

I

Y

Y

G

L

Q

E

N

K

Y

V

V

Y

E

G

E

R

G

W

F

L

G

G

F

F

A

L

L

V

G

G

W

W

N

A

Y

Q

-

M

-

V

-

I

W

Y

Y

Y

N

P

W

A

A

N

V

I

T

A

I

A

A

L

V

A

D

I

L

I

V

F

A

A

S

T

Q

Q

L

F

V

V

M

N

S

L

Y

F

W

A

F

L

P

S

D

D

T

S

-

T

-

I

-

V

P

P

G

T

W

A

I

I

W

L

S

T

A

S

L

I

F

F

L

L

G

-

I

-

M

-

F

M

L

G

L

V

N

N

W

F

I

L

S

G

V

T

R

K

G

Y

F

S

G

G

E

W

A

I

E

Q

Y

T

W

L

F

A

S

T

L

I

I

L

K

K

V

L

A

I

T

P

V

L

I

V

F

I

I

I

I

V

V

A

G

G

V

L

M

L

M

-

I

-

V

-

G

-

I

-

F

Y

K

P

G

G

S

G

Q

G

P

V

T

I

G

R

W

L

S

V

N

P

W

F

G

S

I

V

A

E

D

T

A

H

P

P

F

V

A

L

G

T

G

S

F

F

-

G

S

S

A

A

M

L

I

I

G

A

V

T

A

L

M

-

I

-

V

-

G

-

F
|
F

S
x
A

F

Y

Q
x
D

G

G

T

W

E

I

L

N

I

V

G

G

I

T

A

L

A

A

G

G

E

E

S

M

E

K

D

N

P

P

E

G

K

K

N

M

I

L

P

P

R

K

A

V

V

I

R

I

Q

G

V

G

F

L

W

S

R

I

I

V

L

M

L

A

F

V

Y

Y

V

L

F

L

A

T

I

N

L

I

I

A

I

Y

S

L

L

F

I

V

I

L

P

-

Y

-

T

-

D

D

P

S

S

S

L

Q

L

L

R

A

N

G

D

-

V

-

K

-

D

-

I

-

S

T

V

D

S

T

P

P

F

A

T

A

L

L

V

V

F

A

Q

S

H

H

A

L

G

F

L

E

L

G

S

I

A

G

A

S

A

K

I

L

M

V

N

T

A

I

V

G

I

I

L

L

T

I

A

S

V

V

L

F

S

G

A

G

G

I

N

N

S

G

G

Y

M

I

Y

I

A

S

S

G

T

L

R

R

M

V

L

P

Y

Y

T

A

L

L

A

A

C

T

D

Q

G

K

K

M

A

L

P

P

-

F

-

S

R

D

I

W

F

F

S

A

K

R

L

I

S

N

-

P

R

K

G

T

G

N

V

L

P

P

R

I

N

N

A

G

L

G

Y

L

A

V

T

M

T

L

V

G

I

I

A

A

A

I

L

V

C

M

F

I

L

L

T

T

S

G

M

Q

F

F

G

N

N

Q

Q

L

T

T

V

-

Y

-

L

-

W

-

L

-

N

-

T

-

S

-

G

D

M

L

T

I

G

V

F

F

I

V

A

I

W

W

L

F

G

F

I

I

A

T

I

L

S

T

H

F

Y

I

R

A

-

V

-

I

F

L

R

R

R

K

G

-

Y

-

V

-

M

-

Q

T

G

Q

N

P

D

D

I

I

N

E

N

R

L

-

P

P

Y

Y

R

R

S

V

G

P

F

F

F

Y

P

P

L

V

G

I

P

P

I

L

F

I

A

A

F

I

V

I

L

G

C

G

L

L

I

Y

I

I

T

I

L

F

G

N

-

T

-

L

-

I

-

V

Q

Q

N

P

Y

K

E

N

A

A

F

F

L

I

K

-

D

-

T

-

I

-

D

-

W

-

G

-

G

G

V

I

A

L

T

T

Y

L

I

I

G

G

I

I

P

P

L

I

F

Y

binding alpha-aminoisobutyric acid: V17 (≠ S30), G19 (= G32), A20 (≠ T33), G21 (= G34), F199 (= F216), A200 (≠ S217), D202 (≠ Q219)

9h76A Bacterial lat transporter basc in complex with l-ala and nb53 (see paper)
22% identity, 89% coverage: 25:460/489 of query aligns to 10:422/430 of 9h76A

query
sites
9h76A

I

V

A

V

I

V

G

G

G

T

S

V

I

I

G
|
G

T
x
A

G
|
G

L

I

F

F

V

F

A

K

S

P

G

T

A

A

T

V

I

Y

S

G

Q

A

A

A

G

G

-

A

P

P

G

G

G

L

A

G

L

L

S

A

Y

W

I

F

L

V

I

A

G

G

L

I

M

I

V

T

Y

I

F

A

L

G

M

G

T

L

S

T

L

V

G

A

E

E

L

I

A

G

A

T

F

I

M

Y

P

P

V

Q

S

T

G

G

S

G

F

M

A

M

T

I

Y

Y

G

L

Q

E

N

K

Y

V

V

Y

E

G

E

R

G

W

F

L

G

G

F

F

A

L

L

V

G

G

W

W

N

A

Y

Q

-

M

-

V

-

I

W

Y

Y

Y

N

P

W

A

A

N

V

I

T

A

I

A

A

L

V

A

D

I

L

I

V

F

A

A

S

T

Q

Q

L

F

V

V

M

N

S

L

Y

F

W

A

F

L

P

S

D

D

T

S

-

T

-

I

-

V

P

P

G

T

W

A

I

I

W

L

S

T

A

S

L

I

F

F

L

L

G

-

I

-

M

-

F

M

L

G

L

V

N

N

W

F

I

L

S

G

V

T

R

K

G

Y

F

S

G

G

E

W

A

I

E

Q

Y

T

W

L

F

A

S

T

L

I

I

L

K

K

V

L

A

I

T

P

V

L

I

V

F

I

I

I

I

V

V

A

G

G

V

L

M

L

M

-

I

-

V

-

G

-

I

-

F

Y

K

P

G

G

S

G

Q

G

P

V

T

I

G

R

W

L

S

V

N

P

W

F

G

S

I

V

A

E

D

T

A

H

P

P

F

V

A

L

G

T

G

S

F

F

-

G

S

S

A

A

M

L

I

I

G

A

V

T

A

L

M

-

I

-

V

-

G

-

F

F

S

A

F

Y

Q
x
D

G

G

T

W

E

I

L

N

I

V

G

G

I

T

A

L

A

A

G

G

E

E

S

M

E

K

D

N

P

P

E

G

K

K

N

M

I

L

P

P

R

K

A

V

V

I

R

I

Q

G

V

G

F

L

W

S

R

I

I

V

L

M

L

A

F

V

Y

Y

V

L

F

L

A

T

I

N

L

I

I

A

I

Y

S

L

L

F

I

V

I

L

P

-

Y

-

T

-

D

D

P

S

S

S

L

Q

L

L

R

A

N

G

D

-

V

-

K

-

D

-

I

-

S

T

V

D

S

T

P

P

F

A

T

A

L

L

V

V

F

A

Q

S

H

H

A

L

G

F

L

E

L

G

S

I

A

G

A

S

A

K

I

L

M

V

N

T

A

I

V

G

I

I

L

L

T

I

A

S

V

V

L

F

S

G

A

G

G

I

N

N

S

G

G

Y

M

I

Y

I

A

S

S

G

T

L

R

R

M

V

L

P

Y

Y

T

A

L

L

A

A

C

T

D

Q

G

K

K

M

A

L

P

P

-

F

-

S

R

D

I

W

F

F

S

A

K

R

L

I

S

N

-

P

R

K

G

T

G

N

V

L

P

P

R

I

N

N

A

G

L

G

Y

L

A

V

T

M

T

L

V

G

I

I

A

A

A

I

L

V

C

M

F

I

L

L

T

T

S

G

M

Q

F

F

G

N

N

Q

Q

L

T

T

V

-

Y

-

L

-

W

-

L

-

N

-

T

-

S

-

G

D

M

L

T

I

G

V

F

F

I

V

A

I

W

W

L

F

G

F

I

I

A

T

I

L

S

T

H

F

Y

I

R

A

-

V

-

I

F

L

R

R

R

K

G

-

Y

-

V

-

M

-

Q

T

G

Q

N

P

D

D

I

I

N

E

N

R

L

-

P

P

Y

Y

R

R

S

V

G

P

F

F

F

Y

P

P

L

V

G

I

P

P

I

L

F

I

A

A

F

I

V

I

L

G

C

G

L

L

I

Y

I

I

T

I

L

F

G

N

-

T

-

L

-

I

-

V

Q

Q

N

P

Y

K

E

N

A

A

F

F

L

I

K

-

D

-

T

-

I

-

D

-

W

-

G

-

G

G

V

I

A

L

T

T

Y

L

I

I

G

G

I

I

P

P

L

I

F

Y

binding alanine: G17 (= G32), A18 (≠ T33), G19 (= G34), D200 (≠ Q219)

O34739 Serine/threonine exchanger SteT from Bacillus subtilis (strain 168) (see paper)
26% identity, 51% coverage: 8:255/489 of query aligns to 3:242/438 of O34739

query
sites
O34739

T

T

E

E

A

D

P

N

T

G

L

L

R

K

E

E

L

I

K

G

A

L

R

L

H

F

L

A

T

L

M

T

I

L

A

V

I

I

G

G

G

T

S

I

I

I

G

G

T

S

G

G

L

V

F

F

V

M

A

K

S

P

G

G

A

A

T

V

I

L

S

A

Q

Y

A

S

G

G

P

D

G

S

G

K

-

M

A

A

L

L

L

F

S

A

Y

W

I

L

L

L

I

G

G

G

L

I

M

L

V

T

Y

L

F

A

L

G

M

G

T

L

S

T

L

I

G

A

E

E

L

I

A

G

A

T

F

Q

M

I

P

P

V

K

S

T

G

G

S

G

F

L

A

Y

T

T

Y

Y

G

L

Q

E

N

E

Y

V

V

Y

E

G

E

E

G

F

F

W

G

G

F

F

A

L

L
x
C

G

G

W

W

N

V

Y

Q

-

I

W

I

Y

Y

N

G

W

P

A

A

V

I

T

I

I

G

A

A

V

L

D

G

L

L

V

Y

A

F

S

G

Q

S

L

L

V

M

M

A

S

N

Y

L

W

F

F

-

P

-

D

-

T

-

P

-

G

G

W

W

-

G

-

S

-

G

-

L

-

S

-

K

-

V

-

I

-

G

I

I

W

I

S

A

A

V

L

L

F

F

L

L

G
x
C

I

V

M

-

F

-

L

-

L

I

N

N

W

I

I

I

S

G

V

T

R

K

G

Y

F

G

G

G

E

F

A

V

E

Q

Y

T

W

L

F

T

S

T

-

I

-

G

-

K

L

L

I

I

K

P

V

I

A

A

T
x
C

V

I

I

I

I

V

F

F

I

-

V

-

G

-

V

-

M

-

I

-

V

-

G

G

I

L

F

W

K

K

G

G

S

D

Q

Q

P

H

T

I

G

F

W

T

S

A

-

V

N

N

W

E

G

S

I

I

A

S

D

D

A

M

P

N

F

F

A

G

G

A

F

I

S

L

A

A

M

T

I

L

G

-

V

-

A

-

M

-

I

-

V

-

G

-

F

F

S

A

F

Y

Q

D

G

G

T

W

E

I

L

L

I

L

G

A

I

A

A

L

A

G

G

G

E

E

S

M

E

K

D

N

P

P

E

E

K

K

N

L

I

L

P

P

R

R

A

A

V

M

R

T

Q

G

V

G

F

L

W

L

R

I

I

V

L

T

L

A

F

I

Y

Y

V

I

F

F

C94 (≠ L98) mutation to S: Retains 25% of the transport activity; when associated with S-141; S-168; S-291 and S-415.
C141 (≠ G139) mutation to S: Retains 25% of the transport activity; when associated with S-94; S-168; S-291 and S-415.
C168 (≠ T166) mutation to S: Retains 25% of the transport activity; when associated with S-94; S-141; S-291 and S-415.

Sites not aligning to the query:

291 C→S: Retains 25% of the transport activity; when associated with S-94; S-141; S-168 and S-415.
415 C→S: Retains 25% of the transport activity; when associated with S-94; S-141; S-168 and S-291.

Q22397 Amino acid transporter protein 6 from Caenorhabditis elegans (see paper)
23% identity, 69% coverage: 27:361/489 of query aligns to 30:362/523 of Q22397

query
sites
Q22397

I

V

G

G

G

N

S

I

I

V

G

G

T

S

G

G

L

I

F

F

V

I

A

T

S

P

G

T

A

S

T

I

I

I

S

E

Q

N

A

V

G

N

P

S

G

V

G

G

A

-

L

-

S

-

Y

-

I

-

L

-

I

-

G

-

L

-

M

-

V

-

Y

-

F

-

L

L

M

S

T

L

S

A

L

I

G

W

E

I

L

L

A

A

F

F

M

I

P

S

V

M

S

L

G

G

S

S

F

F

A

C

-

Y

-

V

T

E

Y

L

G

G

Q

T

N

S

Y

I

V

R

E

L

E

S

G

G

F

G

G

D

F

F

A

A

-

Y

L

L

G

C

W

F

N

M

Y

K

W

W

Y

Y

-

P

-

V

-

A

-

F

-

A

-

F

-

M

-

C

-

I

-

G

-

C

-

T

-

I

N

N

W

Y

A

P

V

A

T

T

I

L

A

A

V

V

D

Q

L

-

V

-

A

-

S

A

Q

Q

L

T

V

F

M

A

S

E

Y

Y

W

V

F

F

P

R

-

G

-

A

-

G

-

V

-

E

-

L

D

D

T

E

P

T

G

S

W

E

I

F

W

W

S

A

A

K

L

K

F

L

L

L

G

G

-

F

-

S

-

L

-

I

I

L

M

L

F

M

L

F

L

M

N

N

W

F

I

F

S

S

V

L

R

K

G

T

F

F

G

V

E

Q

A

R

-

F

E

S

Y

I

W

L

F

A

S

S

L

L

I

A

K

K

V

I

A

A

T

A

V

T

I

L

F

I

I

I

V

T

G

G

V

F

M

Y

I

L

V

-

G

-

I

I

F

F

K

K

G

H

-

W

-

K

-

Q

-

N

-

L

S

E

Q

E

P

P

T

F

G

K

W

G

S

S

N

N

W

W

G

N

I

-

A

-

D

P

A

G

P

P

F

F

A

V

G

N

G

A

F

L

S

F

A

A

M

G

I

L

G

-

V

-

A

-

M

-

I

-

V

-

G

-

F

F

S

S

F

Y

Q

D

G

G

T

W

E

D

L

I

I

L

G

N

I

F

A

G

A

A

G

E

E

E

S

I

E

E

D

N

P

P

E

K

K

R

N

T

I

M

P

P

R

L

A

S

V

I

R

-

Q

-

V

-

F

-

W

-

R

-

I

I

L

I

L

G

F

M

Y

T

V

C

F

I

A

G

I

V

L

I

I

Y

I

V

S

A

L

V

I

N

I

V

P

A

Y

Y

T

-

D

-

P

-

S

S

L

I

L

V

R

L

N

S

D

P

V

T

K

E

D

M

I

I

S

A

V

S

S

N

P

A

F

V

T

A

L

I

V

D

F

F

Q

A

H

N

A

K

G

T

L

L

L

G

S

A

A

A

A

F

I

V

M

V

N

P

A

V

V

M

I

V

L

A

T

I

A

L

V

L

L

I

S

G

A

S

G

L

N

N

S

S

G

T

M

M

Y

F

A

S

S

A

T

S

R

R

M

Y

L

L

Y

Q

T

A

L

V

A

S

C

R

D

Q

G

G

K

H

A

I

P

P

R

S

I

A

F

I

S

S

K

G

L

I

S

A

-

P

R

N

G

C

G

D

V

S

P

P

R

R

N

V

A

A

L

L

Y

L

A

V

T

H

T

I

V

L

I

I

A

A

-

I

A

A

L

V

C

S

F

F

L

L

Sites not aligning to the query:

521:523 PDZ-binding motif; mutation Missing: Abolishes the interaction with nrfl-1.

Q9QXW9 Large neutral amino acids transporter small subunit 2; L-type amino acid transporter 2; mLAT2; Solute carrier family 7 member 8 from Mus musculus (Mouse) (see paper)
25% identity, 72% coverage: 13:363/489 of query aligns to 35:381/531 of Q9QXW9

query
sites
Q9QXW9

L

L

R

K

E

E

L

I

K

G

A

L

R

V

H

S

L

A

T

C

M

G

I

I

A

I

I

V

G

G

G

N

S

I

I

I

G

G

T

S

G

G

L

I

F

F

V

V

A

S

S

P

G

K

A

G

T

V

I

L

S

E

Q

N

A

A

G

G

P

S

G

V

G

G

-

L

A

A

L

L

L

I

S

V

Y

W

I

I

L

V

I

T

G

G

L

I

M

I

V

T

Y

A

F

V

L

G

M

A

T

L

S

C

L

Y

G

A

E

E

L

L

A

G

A

V

F

T

M

I

P

P

V

K

S

S

G

G

S

G

F

D

A

Y

T

S

Y

Y

G

V

Q

K

N

D

Y

I

V

F

E

G

G

L

F

A

G

G

F

F

A

L

L

R

G

L

W

W

N

I

-

A

-

V

-

L

-

V

-

I

Y
|
Y

W

P

Y

T

N
|
N

W

Q

A

A

V

V

T

-

I

-

A

-

V

I

D

A

L

L

V

T

A

F

S

S

Q

N

L

Y

V

V

M

L

S

Q

Y

P

W

L

F

F

P

P

D

T

T

C

-

F

-

P

P

P

G

E

W

S

I

G

W

L

S

R

A

L

L

L

F

A

L

A

G

I

I

C

M

L

F

L

L

L

N

T

W

W

I

V

-

N

-

C

-

S

S

S

V

V

R

R

G

W

F

A

G

T

E

R

A

V

E

Q

Y

D

W

I

F

F

S

T

L

A

I

G

K

K

V

L

A

L

T

A

V

L

I

A

I

L

F

I

I

I

V

M

G

G

V

I

M

V

M

Q

I

I

V

C

-

K

G

G

I

E

F

F

K

F

G

W

S

L

Q

E

P

P

-

K

T

N

G

A

W

F

S

E

N

N

W

F

G

Q

I

E

A

P

D

D

A

I

P

-

F

-

A

-

G

-

F

-

S

-

A

G

M

L

I

V

G

A

V

L

A

A

M

F

I

L

V

Q

G

G

-

S

F
|
F

S

A

F

Y

Q

G

G

G

T

W

E

N

L

F

I

L

G

N

I

Y

A

V

A

T

G

E

E

E

S

L

E

V

D

D

P

P

E

Y

K

K

N

N

I

L

P

P

R

R

A

A

V

I

R

F

Q

I

V

S

F

I

W

P

R

L

I

V

L

T

L

F

F

V

Y

Y

V

V

F

F

A

A

I

-

L

-

I

N

I

I

S

A

L

Y

I

V

I

T

P

A

Y

M

T

S

D

P

P

Q

S

E

L

L

R

A

N

S

D

N

V

A

K

V

D

A

I

V

S

T

V

-

S

-

P

-

F

-

T

-

L

-

V

-

F

F

Q

G

H

E

A

K

G

L

L

L

L

G

S

V

A

M

A

A

A

W

I

I

M

M

N

P

A

I

V

S

I

V

L

A

T

L

A

S

V

T

L

F

S

G

A

G

G

V

N

N

S

G

G

S

M

L

Y

F

A

T

S

S

T

S

R

R

M

L

L

F

Y

F

T

A

L

G

A

A

C

R

D

E

G

G

K

H

A

L

P

P

R

S

I

V

F

L

S

A

K

M

L

I

S

H

-

V

R

K

G

R

G

C

V

T

P

P

R

I

N

P

A

A

L

L

Y

L

A

F

T

T

T

C

V

L

I

S

A

T

A

L

L

L

C

M

F

L

L

V

T

T

S

S

Y130 (= Y102) mutation to A: Increases T2 import. Increases T3 and enables T4 import. Does not affect L-leucine and L-phenylalanine uptake.
N133 (= N105) mutation to S: Increases T2 import. Does not affect T3 import. Does not affect L-leucine and L-phenylalanine uptake. Increases the export of both L-leucine and L-phenylalanine.
F242 (= F216) mutation to W: Increases T2 import. Does not affect T3 import. Does not affect L-leucine and L-phenylalanine uptake.

Q9UHI5 Large neutral amino acids transporter small subunit 2; L-type amino acid transporter 2; hLAT2; Solute carrier family 7 member 8 from Homo sapiens (Human) (see 3 papers)
25% identity, 72% coverage: 13:363/489 of query aligns to 36:382/535 of Q9UHI5

query
sites
Q9UHI5

L

L

R

K

E

E

L

I

K

G

A

L

R

V

H

S

L

A

T

C

M

G

I

I

A

I

I

V

G

G

G

N

S
x
I

I

I

G

G

T

S

G

G

L

I

F

F

V

V

A

S

S

P

G

K

A

G

T

V

I

L

S

E

Q

N

A

A

G

G

P

S

G

V

G

G

-

L

A

A

L

L

L

I

S

V

Y

W

I

I

L

V

I

T

G

G

L

F

M

I

V

T

Y

V

F

V

L

G

M

A

T

L

S

C

L
x
Y

G

A

E

E

L

L

A

G

A

V

F

T

M

I

P

P

V

K

S

S

G

G

S

G

F

D

A

Y

T

S

Y

Y

G

V

Q

K

N

D

Y

I

V

F

E

G

G

L

F

A

G

G

F

F

A

L

L

R

G

L

W

W

N

I

-

A

-

V

-

L

-

V

-

I

Y

Y

W

P

Y

T

N
|
N

W

Q

A

A

V

V

T

-

I

-

A

-

V

I

D

A

L

L

V

T

A

F

S

S

Q

N

L

Y

V

V

M

L

S

Q

Y

P

W

L

F

F

P

P

D

T

T
x
C

-

F

-

P

P

P

G

E

W

S

I

G

W

L

S

R

A

L

L

L

F

A

L

A

G

I

I

C

M

L

F

L

L

L

N

T

W
|
W

I

V

-

N

-

C

-

S

S

S

V

V

R

R

G

W

F

A

G

T

E

R

A

V

E

Q

Y

D

W

I

F

F

S

T

L

A

I

G

K

K

V

L

A

L

T

A

V

L

I

A

I

L

F

I

I

I

V

M

G

G

V

-

M

-

I

I

V

V

G

Q

I

I

F

C

K

K

G

G

S

E

Q

Y

P

-

T

-

G

F

W

W

S

L

N

E

W

P

G

K

I

N

A

A

D

F

A

E

P

N

F

F

A

Q

G

E

G

P

F

D

S

I

A

G

M

L

I

V

G

A

V

L

A

A

M

F

I

L

V

Q

G

G

-

S

F
|
F

S

A

F

Y

Q
x
G

G

G

T

W

E

N

L

F

I

L

G

N

I

Y

A

V

A

T

G

E

E

E

S

L

E

V

D

D

P

P

E

Y

K

K

N

N

I

L

P

P

R

R

A

A

V

I

R

F

Q

I

V

S

F

I

W

P

R

L

I

V

L

T

L

F

F

V

Y

Y

V

V

F

F

A

A

I

-

L

-

I

N

I

V

S

A

L

Y

I

V

I

T

P

A

Y

M

T

S

D

P

P

Q

S

E

L

L

R

A

N

S

D

N

V

A

K
x
V

D

A

I

V

S

T

V

-

S

-

P

-

F

-

T

-

L

-

V

-

F

F

Q

G

H

E

A

K

G

L

L

L

L

G

S

V

A

M

A

A

A

W

I

I

M

M

N

P

A

I

V

S

I

V

L

A

T

L

A

S

V

T

L

F

S

G

A

G

G

V

N

N

S

G

G

S

M

L

Y

F

A

T

S

S

T

S

R

R

M

L

L

F

Y

F

T

A

L

G

A

A

C

R

D

E

G

G

K

H

A

L

P

P

R

S

I

V

F

L

S

A

K

M

L

I

S

H

-

V

R

K

G

R

G

C

V

T

P

P

R

I

N

P

A

A

L

L

Y

L

A

F

T

T

T

C

V

I

I

S

A

T

A

L

L

L

C

M

F

L

L

V

T

T

S

S

I53 (≠ S30) binding L-leucine
Y93 (≠ L69) mutation to A: Nearly complete reduction of glycine, L-alanine, and L-glutamine uptake. Minimal effect on the transport of L-isoleucine, L-histidine and L-tryptophan.
N134 (= N105) Important for substrate specificity; binding L-tryptophan; mutation to Q: Reduces L-leucine uptake activity. Abolishes L-tryptophan uptake.; mutation to S: The substrate specificity changed dramatically reducing L-glutamine, glycine and L-alanine uptake activity thus mimicking the selectivity of SLC7A5.
C154 (≠ T128) modified: Interchain (with C-210 in SLC3A2)
W174 (= W146) mutation to A: Does not affect protein expression, plasma membrane localization, or L-alanine uptake.
F243 (= F216) mutation to A: Abolishes leucine and tryptophan transport activities.
G246 (≠ Q219) Important for substrate specificity; binding L-leucine; mutation to S: Strong decrease in the uptake of large substrates L-tryptophan, L-glutamine, and L-histidine but increases the uptake of small neutral amino acids glycine and L-alanine.
V302 (≠ K277) to I: found in a patient with age-related hearing loss; does not affect L-alanine transport activity. Decreases L-tyrosine transport activity; dbSNP:rs142951280

Sites not aligning to the query:

395 binding L-tryptophan; N→Q: Strongly reduces L-leucine uptake activity. Strongly reduces L-tryptophan uptake activity.
396 Y→A: Strongly reduces L-leucine uptake activity.
402 T → M: found in a patient with age-related hearing loss; strongly decreased L-alanine transport activity. Decreases L-tyrosine transport activity; dbSNP:rs758342760
418 R → C: found in a patient with age-related hearing loss; decreases L-alanine transport activity. Decreases L-tyrosine transport activity; dbSNP:rs146946494
460 V → E: found in a patient with age-related hearing loss; strongly decreases L-alanine transport activity. Decreases L-tyrosine transport activity. Decreases cell membrane localization; dbSNP:rs2048595742

Query Sequence

>WP_008804022.1 NCBI__GCF_000025465.1:WP_008804022.1
MVSETKTTEAPTLRRELKARHLTMIAIGGSIGTGLFVASGATISQAGPGGALLSYILIGL
MVYFLMTSLGELAAFMPVSGSFATYGQNYVEEGFGFALGWNYWYNWAVTIAVDLVASQLV
MSYWFPDTPGWIWSALFLGIMFLLNWISVRGFGEAEYWFSLIKVATVIIFIIVGVMMIVG
IFKGSQPTGWSNWGIADAPFAGGFSAMIGVAMIVGFSFQGTELIGIAAGESEDPEKNIPR
AVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDVKDISVSPFTLVFQHAGLLSAAAIM
NAVILTAVLSAGNSGMYASTRMLYTLACDGKAPRIFSKLSRGGVPRNALYATTVIAALCF
LTSMFGNQTVYLWLLNTSGMTGFIAWLGIAISHYRFRRGYVMQGNDINNLPYRSGFFPLG
PIFAFVLCLIITLGQNYEAFLKDTIDWGGVAATYIGIPLFLVIWFGYKLAKGTRFVRYSE
MTFPDRFKR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory