SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_008805274.1 NCBI__GCF_000025465.1:WP_008805274.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P22256 4-aminobutyrate aminotransferase GabT; 5-aminovalerate transaminase; GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase; L-AIBAT; EC 2.6.1.19; EC 2.6.1.48 from Escherichia coli (strain K12) (see 2 papers)
89% identity, 100% coverage: 1:426/427 of query aligns to 1:426/426 of P22256

query
sites
P22256

M

M

N

N

S

S

N

N

K

K

A

E

M

L

M

M

A

Q

R

R

S

S

D

Q

A

A

V

I

P

P

R

R

G

G

V

V

G

G

Q

Q

I

I

H

H

P

P

I

I

F

F

A

A

E

D

R

R

A

A

E

E

N

N

C

C

R

R

V

V

W

W

D

D

V

V

E

E

G

G

R

R

E

E

Y

Y

L

L

D

D

F

F

A

A

G

G

I
|
I

A

A

V

V

L

L

N

N

T

T

G

G

H

H

L

L

H

H

P

P

Q

K

V

V

A

A

V

V

E

E

D

A

Q

Q

L

L

K

K

L

L

S

S

H

H

T

T

C

C

F

F

Q

Q

V

V

L

L

A

A

Y

Y

E

E

P

P

Y

Y

L

L

A

E

L

L

C

C

E

E

K

I

M

M

N

N

Q

Q

K

K

V

V

P

P

G

G

D

D

F

F

A

A

K

K

T

T

L

L

V

V

T

T

G
|
G

S
|
S

E

E

A

A

V

V

E

E

N

N

A

A

V

V

K

K

I

I

A

A

R

R

A

A

T

T

G

K

R

R

S

S

G

G

A

T

I

I

A

A

F

F

T

S

G

G

A

A

Y

Y

H

H

G

G

R

R

T

T

H

H

Y

Y

T

T

L

L

S

A

L

L

T

T

G

G

K

K

V

V

N

N

P

P

Y

Y

S

S

A

A

G

G

M

M

G

G

L

L

M

M

P

P

G

G

H

H

V

V

Y

Y

R

R

A

A

L

L

Y

Y

P

P

C

C

A

P

L

L

H

H

G

G

V

I

S

S

D

E

D

D

E

D

A

A

I

I

A

A

S

S

I

I

H

H

R

R

I

I

F

F

K

K

N

N

D

D

A

A

P

P

E

E

D

D

I

I

A

A

I

I

I

V

I

I

E

E

P

P

V

V

Q

Q

G

G

E
|
E

G

G

F

F

Y

Y

A

A

A

S

S

S

P

P

A

A

F

F

M

M

Q

Q

R

R

L

L

R

R

A

A

L

L

C

C

D

D

E

E

H

H

G

G

I

I

M

M

L

L

I

I

A

A

D

D

E

E

V
|
V

Q
|
Q

S

S

G

G

A

A

G

G

R

R

T

T

G

G

T

T

L

L

F

F

A

A

M

M

E

E

Q

Q

M

M

G

G

V

V

A

A

A

P

D

D

I

L

T

T

F

F

A

A

K
|
K

S

S

I

I

A

A

G

G

F

F

P

P

L

L

A

A

G

G

V

V

T

T

G

G

R

R

A

A

E

E

V

V

M

M

D

D

A

A

I

V

A

A

P

P

G

G

L

L

G

G

T
|
T

Y

Y

A

A

G

G

N

N

P

P

I

I

A

A

C

C

A

V

A

A

L

L

A

E

V

V

L

L

Q

K

I

V

F

F

E

E

Q

Q

E

E

N

N

L

L

E

Q

K

K

A

A

N

N

Q

D

L

L

G

G

D

Q

T

K

L

L

R

K

Q

D

G

G

L

L

A

A

I

I

A

A

E

E

D

K

H

H

P

P

E

E

I

I

G

G

D

D

V

V

R

R

G

G

L

L

G

G

A

A

M

M

I

I

A

A

I

I

E

E

L

L

F

F

E

E

E

D

G

G

D

D

R

H

S

N

K

K

P

P

N

D

A

A

R

K

L

L

T

T

A

A

D

E

I

I

V

V

A

A

R

R

A

A

R

R

D

D

K

K

G

G

L

L

I

I

L

L

S

S

C

C

G

G

P

P

Y

Y

N

N

V

V

L

L

R

R

I

I

L

L

V

V

P

P

L

L

T

T

I

I

E

E

E

D

A

A

Q

Q

I

I

E

R

Q

Q

G

G

L

L

K

E

I

I

A

S

D

Q

C

C

F

F

T

D

E

E

A

A

K

K

Q

Q

I50 (= I50) mutation to Q: 3-fold decrease in catalytic activity and 12-fold decrease in affinity for GABA.
GS 111:112 (= GS 111:112) binding pyridoxal 5'-phosphate
E211 (= E211) mutation to S: 100-fold decrease in catalytic activity and 15-fold decrease in affinity for GABA.
V241 (= V241) mutation to A: 25-fold decrease in catalytic activity and 5-fold decrease in affinity for GABA.
Q242 (= Q242) binding pyridoxal 5'-phosphate
K268 (= K268) modified: N6-(pyridoxal phosphate)lysine
T297 (= T297) binding pyridoxal 5'-phosphate

1sffA Structure of gamma-aminobutyrate aminotransferase complex with aminooxyacetate (see paper)
89% identity, 100% coverage: 2:426/427 of query aligns to 1:425/425 of 1sffA

query
sites
1sffA

N

N

S

S

N

N

K

K

A

E

M

L

M

M

A

Q

R

R

S

S

D

Q

A

A

V

I

P

P

R

R

G

G

V
|
V

G

G

Q

Q

I

I

H

H

P

P

I

I

F

F

A

A

E

D

R

R

A

A

E

E

N

N

C

C

R

R

V

V

W

W

D

D

V

V

E

E

G

G

R

R

E

E

Y

Y

L

L

D

D

F

F

A

A

G

G

I

I

A

A

V

V

L

L

N

N

T

T

G

G

H

H

L

L

H

H

P

P

Q

K

V

V

A

A

V

V

E

E

D

A

Q

Q

L

L

K

K

L

L

S

S

H

H

T

T

C

C

F

F

Q
|
Q

V

V

L

L

A

A

Y

Y

E

E

P

P

Y

Y

L

L

A

E

L

L

C

C

E

E

K

I

M

M

N

N

Q

Q

K

K

V

V

P

P

G

G

D

D

F

F

A

A

K

K

T

T

L

L

V

V

T

T

G
|
G

S
|
S

E

E

A

A

V

V

E

E

N

N

A

A

V

V

K

K

I

I

A

A

R

R

A

A

T

T

G

K

R

R

S

S

G

G

A

T

I

I

A

A

F

F

T

S

G

G

A

A

Y
|
Y

H
|
H

G

G

R
|
R

T

T

H

H

Y

Y

T

T

L

L

S

A

L

L

T

T

G

G

K

K

V

V

N
|
N

P

P

Y

Y

S

S

A

A

G

G

M

M

G

G

L

L

M

M

P

P

G

G

H

H

V

V

Y

Y

R

R

A

A

L

L

Y

Y

P

P

C

C

A

P

L

L

H

H

G

G

V

I

S

S

D

E

D

D

E

D

A

A

I

I

A

A

S

S

I

I

H

H

R

R

I

I

F

F

K

K

N

N

D

D

A

A

P

P

E

E

D

D

I

I

A

A

I

I

I

V

I

I

E
|
E

P

P

V

V

Q

Q

G

G

E

E

G

G

F

F

Y

Y

A

A

A

S

S

S

P

P

A

A

F

F

M

M

Q

Q

R

R

L

L

R

R

A

A

L

L

C

C

D

D

E

E

H

H

G

G

I

I

M

M

L

L

I

I

A

A

D
|
D

E

E

V
|
V

Q
|
Q

S

S

G

G

A

A

G

G

R

R

T

T

G

G

T

T

L

L

F

F

A

A

M

M

E

E

Q

Q

M

M

G

G

V

V

A

A

A

P

D

D

I

L

T

T

F

F

A

A

K
|
K

S

S

I

I

A

A

G

G

F

F

P

P

L

L

A

A

G

G

V

V

T

T

G

G

R

R

A

A

E

E

V

V

M

M

D

D

A

A

I

V

A

A

P

P

G

G

L

L

G

G

T
|
T

Y

Y

A

A

G

G

N

N

P

P

I

I

A

A

C

C

A

V

A

A

L

L

A

E

V

V

L

L

Q

K

I

V

F

F

E

E

Q

Q

E

E

N

N

L

L

E

Q

K

K

A

A

N

N

Q

D

L

L

G

G

D

Q

T

K

L

L

R

K

Q

D

G

G

L

L

A

A

I

I

A

A

E

E

D

K

H

H

P

P

E

E

I

I

G

G

D

D

V

V

R

R

G

G

L

L

G

G

A

A

M

M

I

I

A

A

I

I

E

E

L

L

F

F

E

E

E

D

G

G

D

D

R

H

S

N

K

K

P

P

N

D

A

A

R

K

L

L

T

T

A

A

D

E

I

I

V

V

A

A

R

R

A

A

R

R

D

D

K

K

G

G

L

L

I

I

L

L

S

S

C

C

G

G

P

P

Y

Y

Y
|
Y

N

N

V

V

L

L

R
|
R

I

I

L

L

V

V

P

P

L

L

T

T

I

I

E

E

E

D

A

A

Q

Q

I

I

E

R

Q

Q

G

G

L

L

K

E

I

I

A

S

D

Q

C

C

F

F

T

D

E

E

A

A

K

K

Q

Q

active site: V18 (= V19), Y137 (= Y138), E205 (= E206), D238 (= D239), Q241 (= Q242), K267 (= K268), T296 (= T297), R397 (= R398)
binding 4'-deoxy-4'-acetylyamino-pyridoxal-5'-phosphate: Q78 (= Q79), G110 (= G111), S111 (= S112), Y137 (= Y138), H138 (= H139), R140 (= R141), E205 (= E206), D238 (= D239), V240 (= V241), Q241 (= Q242), K267 (= K268), T296 (= T297)
binding sulfate ion: N152 (= N153), Y393 (= Y394)

1sf2A Structure of e. Coli gamma-aminobutyrate aminotransferase (see paper)
89% identity, 100% coverage: 2:426/427 of query aligns to 1:425/425 of 1sf2A

query
sites
1sf2A

N

N

S

S

N

N

K

K

A

E

M

L

M

M

A

Q

R

R

S

S

D

Q

A

A

V

I

P

P

R

R

G

G

V
|
V

G

G

Q

Q

I

I

H

H

P

P

I

I

F

F

A

A

E

D

R

R

A

A

E

E

N

N

C

C

R

R

V

V

W

W

D

D

V

V

E

E

G

G

R

R

E

E

Y

Y

L

L

D

D

F

F

A

A

G

G

I

I

A

A

V

V

L

L

N

N

T

T

G

G

H

H

L

L

H

H

P

P

Q

K

V

V

A

A

V

V

E

E

D

A

Q

Q

L

L

K

K

L

L

S

S

H

H

T

T

C

C

F

F

Q

Q

V

V

L

L

A

A

Y

Y

E

E

P

P

Y

Y

L

L

A

E

L

L

C

C

E

E

K

I

M

M

N

N

Q

Q

K

K

V

V

P

P

G

G

D

D

F

F

A

A

K

K

T

T

L

L

V

V

T

T

G
|
G

S
|
S

E

E

A

A

V

V

E

E

N

N

A

A

V

V

K

K

I

I

A

A

R

R

A

A

T

T

G

K

R

R

S

S

G

G

A

T

I

I

A

A

F

F

T

S

G

G

A

A

Y
|
Y

H
|
H

G

G

R

R

T

T

H

H

Y

Y

T

T

L

L

S

A

L

L

T

T

G

G

K

K

V

V

N
|
N

P

P

Y

Y

S

S

A

A

G

G

M

M

G

G

L

L

M

M

P

P

G

G

H

H

V

V

Y

Y

R

R

A

A

L

L

Y

Y

P

P

C

C

A

P

L

L

H

H

G

G

V

I

S

S

D

E

D

D

E

D

A

A

I

I

A

A

S

S

I

I

H

H

R

R

I

I

F

F

K

K

N

N

D

D

A

A

P

P

E

E

D

D

I

I

A

A

I

I

I

V

I

I

E
|
E

P

P

V

V

Q

Q

G

G

E

E

G

G

F

F

Y

Y

A

A

A

S

S

S

P

P

A

A

F

F

M

M

Q

Q

R

R

L

L

R

R

A

A

L

L

C

C

D

D

E

E

H

H

G

G

I

I

M

M

L

L

I

I

A

A

D
|
D

E

E

V
|
V

Q
|
Q

S

S

G

G

A

A

G

G

R

R

T

T

G

G

T

T

L

L

F

F

A

A

M

M

E

E

Q

Q

M

M

G

G

V

V

A

A

A

P

D

D

I

L

T

T

F

F

A

A

K
|
K

S

S

I

I

A

A

G

G

F

F

P

P

L

L

A

A

G

G

V

V

T

T

G

G

R

R

A

A

E

E

V

V

M

M

D

D

A

A

I

V

A

A

P

P

G

G

L

L

G

G

T
|
T

Y

Y

A

A

G

G

N

N

P

P

I

I

A

A

C

C

A

V

A

A

L

L

A

E

V

V

L

L

Q

K

I

V

F

F

E

E

Q

Q

E

E

N

N

L

L

E

Q

K

K

A

A

N

N

Q

D

L

L

G

G

D

Q

T

K

L

L

R

K

Q

D

G

G

L

L

A

A

I

I

A

A

E

E

D

K

H

H

P

P

E

E

I

I

G

G

D

D

V

V

R

R

G

G

L

L

G

G

A

A

M

M

I

I

A

A

I

I

E

E

L

L

F

F

E

E

E

D

G

G

D

D

R

H

S

N

K

K

P

P

N

D

A

A

R

K

L

L

T

T

A

A

D

E

I

I

V

V

A

A

R

R

A

A

R

R

D

D

K

K

G

G

L

L

I

I

L

L

S

S

C

C

G

G

P

P

Y

Y

Y
|
Y

N

N

V

V

L

L

R
|
R

I

I

L

L

V

V

P

P

L

L

T

T

I

I

E

E

E

D

A

A

Q

Q

I

I

E

R

Q

Q

G

G

L

L

K

E

I

I

A

S

D

Q

C

C

F

F

T

D

E

E

A

A

K

K

Q

Q

active site: V18 (= V19), Y137 (= Y138), E205 (= E206), D238 (= D239), Q241 (= Q242), K267 (= K268), T296 (= T297), R397 (= R398)
binding pyridoxal-5'-phosphate: G110 (= G111), S111 (= S112), Y137 (= Y138), H138 (= H139), E205 (= E206), D238 (= D239), V240 (= V241), Q241 (= Q242), K267 (= K268)
binding sulfate ion: N152 (= N153), Y393 (= Y394)

1szkA The structure of gamma-aminobutyrate aminotransferase mutant: e211s (see paper)
89% identity, 100% coverage: 2:426/427 of query aligns to 1:425/425 of 1szkA

query
sites
1szkA

N

N

S

S

N

N

K

K

A

E

M

L

M

M

A

Q

R

R

S

S

D

Q

A

A

V

I

P

P

R

R

G

G

V
|
V

G

G

Q

Q

I

I

H

H

P

P

I

I

F

F

A

A

E

D

R

R

A

A

E

E

N

N

C

C

R

R

V

V

W

W

D

D

V

V

E

E

G

G

R

R

E

E

Y

Y

L

L

D

D

F

F

A

A

G

G

I

I

A

A

V

V

L

L

N

N

T

T

G

G

H

H

L

L

H

H

P

P

Q

K

V

V

A

A

V

V

E

E

D

A

Q

Q

L

L

K

K

L

L

S

S

H

H

T

T

C

C

F

F

Q

Q

V

V

L

L

A

A

Y

Y

E

E

P

P

Y

Y

L

L

A

E

L

L

C

C

E

E

K

I

M

M

N

N

Q

Q

K

K

V

V

P

P

G

G

D

D

F

F

A

A

K

K

T

T

L

L

V

V

T

T

G
|
G

S
|
S

E

E

A

A

V

V

E

E

N

N

A

A

V

V

K

K

I

I

A

A

R

R

A

A

T

T

G

K

R

R

S

S

G

G

A

T

I

I

A

A

F

F

T

S

G

G

A

A

Y
|
Y

H
|
H

G

G

R

R

T

T

H

H

Y

Y

T

T

L

L

S

A

L

L

T

T

G

G

K

K

V

V

N

N

P

P

Y

Y

S

S

A

A

G

G

M

M

G

G

L

L

M

M

P

P

G

G

H

H

V

V

Y

Y

R

R

A

A

L

L

Y

Y

P

P

C

C

A

P

L

L

H

H

G

G

V

I

S

S

D

E

D

D

E

D

A

A

I

I

A

A

S

S

I

I

H

H

R

R

I

I

F

F

K

K

N

N

D

D

A

A

P

P

E

E

D

D

I

I

A

A

I

I

I

V

I

I

E
|
E

P

P

V

V

Q

Q

G

G

E

S

G

G

F

F

Y

Y

A

A

A

S

S

S

P

P

A

A

F

F

M

M

Q

Q

R

R

L

L

R

R

A

A

L

L

C

C

D

D

E

E

H

H

G

G

I

I

M

M

L

L

I

I

A

A

D
|
D

E

E

V
|
V

Q
|
Q

S

S

G

G

A

A

G

G

R

R

T

T

G

G

T

T

L

L

F

F

A

A

M

M

E

E

Q

Q

M

M

G

G

V

V

A

A

A

P

D

D

I

L

T

T

F

F

A

A

K
|
K

S

S

I

I

A

A

G

G

F

F

P

P

L

L

A

A

G

G

V

V

T

T

G

G

R

R

A

A

E

E

V

V

M

M

D

D

A

A

I

V

A

A

P

P

G

G

L

L

G

G

T
|
T

Y

Y

A

A

G

G

N

N

P

P

I

I

A

A

C

C

A

V

A

A

L

L

A

E

V

V

L

L

Q

K

I

V

F

F

E

E

Q

Q

E

E

N

N

L

L

E

Q

K

K

A

A

N

N

Q

D

L

L

G

G

D

Q

T

K

L

L

R

K

Q

D

G

G

L

L

A

A

I

I

A

A

E

E

D

K

H

H

P

P

E

E

I

I

G

G

D

D

V

V

R

R

G

G

L

L

G

G

A

A

M

M

I

I

A

A

I

I

E

E

L

L

F

F

E

E

E

D

G

G

D

D

R

H

S

N

K

K

P

P

N

D

A

A

R

K

L

L

T

T

A

A

D

E

I

I

V

V

A

A

R

R

A

A

R

R

D

D

K

K

G

G

L

L

I

I

L

L

S

S

C

C

G

G

P

P

Y

Y

N

N

V

V

L

L

R
|
R

I

I

L

L

V

V

P

P

L

L

T

T

I

I

E

E

E

D

A

A

Q

Q

I

I

E

R

Q

Q

G

G

L

L

K

E

I

I

A

S

D

Q

C

C

F

F

T

D

E

E

A

A

K

K

Q

Q

active site: V18 (= V19), Y137 (= Y138), E205 (= E206), D238 (= D239), Q241 (= Q242), K267 (= K268), T296 (= T297), R397 (= R398)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G110 (= G111), S111 (= S112), Y137 (= Y138), H138 (= H139), E205 (= E206), D238 (= D239), V240 (= V241), Q241 (= Q242), K267 (= K268)

P50457 4-aminobutyrate aminotransferase PuuE; GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase; EC 2.6.1.19 from Escherichia coli (strain K12) (see paper)
55% identity, 99% coverage: 3:423/427 of query aligns to 2:421/421 of P50457

query
sites
P50457

S

S

N

N

K

N

A

E

M

F

M

H

A

Q

R

R

S

L

D

S

A

A

V

T

P

P

R

R

G

G

V

V

G

G

Q

V

I

M

H

C

P

N

I

F

F

F

A

A

E

Q

R

S

A

A

E

E

N

N

C

A

R

T

V

L

W

K

D

D

V

V

E

E

G

G

R

N

E

E

Y

Y

L

I

D

D

F

F

A

A

G

A

G

G

I

I

A

A

V

V

L

L

N

N

T

T

G

G

H

H

L

R

H

H

P

P

Q

D

V

L

V

V

A

A

V

V

E

E

D

Q

Q

Q

L

L

K

Q

L

F

S

T

H

H

T

T

C

A

F

Y

Q

Q

V

I

L

V

A

P

Y

Y

E

E

P

S

Y

Y

L

V

A

T

L

L

C

A

E

E

K

K

M

I

N

N

Q

A

K

L

V

A

P

P

G

V

D

S

F

G

A

Q

K

A

K

K

T

T

L

A

L

F

V

F

T

T

G

G

S

A

E

E

A

A

V

V

E

E

N

N

A

A

V

V

K

K

I

I

A

A

R

R

A

A

A

H

T

T

G

G

R

R

S

P

G

G

A

V

I

I

A

A

F

F

T

S

G

G

A

G

Y

F

H

H

G

G

R

R

T

T

H

Y

Y

M

T

T

L

M

S

A

L

L

T

T

G

G

K

K

V

V

N

A

P

P

Y

Y

S

K

A

I

G

G

M

F

G

G

L

P

M

F

P

P

G

G

H

S

V

V

Y

Y

R

H

A

V

L

P

Y

Y

P

P

C

S

A

D

L

L

H

H

G

G

V

I

S

S

D

T

D

Q

E

D

A

S

I

L

A

D

S

A

I

I

H

E

R

R

I

L

F

F

K

K

N

S

D

D

A

I

A

E

P

A

E

K

D

Q

I

V

A

A

I

I

I

F

E

E

P

P

V

V

Q

Q

G

G

E

E

G

G

F

F

Y

N

A

V

A

A

S

P

P

K

A

E

F

L

M

V

Q

A

R

A

L

I

R

R

A

R

L

L

C

C

D

D

E

E

H

H

G

G

I

I

M

V

L

M

I

I

A

A

D

D

E

E

V

V

Q

Q

S

S

G

G

A

F

G

A

R

R

T

T

G

G

T

K

L

L

F

F

A

A

M

M

E

D

Q

H

M

Y

G

A

V

D

A

K

A

P

D

D

I

L

T

M

T

T

F

M

A

A

K
|
K

S

S

I

L

A

A

G

G

F

M

P

P

L

L

A

S

G

G

V

V

T

V

G

G

R

N

A

A

E

N

V

I

M

M

D

D

A

A

I

P

A

A

P

P

G

G

L

L

G

G

T

T

Y

Y

A

A

G

G

N

N

P

P

I

L

A

A

C

V

A

A

L

H

A

A

V

V

L

L

Q

N

I

I

F

I

E

D

Q

K

E

E

N

S

L

L

L

C

E

E

K

R

A

A

N

N

Q

Q

L

L

G

G

D

Q

T

R

L

L

R

K

Q

N

G

T

L

L

L

I

A

D

I

A

A

K

E

E

D

S

H

V

P

P

E

A

I

I

G

A

D

A

V

V

R

R

G

G

L

L

G

G

A

S

M

M

I

I

A

A

I

V

E

E

L

-

F

F

E

N

E

D

G

P

D

Q

R

T

S

G

K

E

P

P

N

S

A

A

R

A

L

I

T

A

A

Q

D

K

I

I

V

Q

A

Q

R

R

A

A

R

L

D

A

K

Q

G

G

L

L

I

L

L

L

S

T

C

C

G

G

P

A

Y

Y

Y

G

N

N

V

V

L

I

R

R

I

F

L

L

V

Y

P

P

L

L

T

T

I

I

E

P

E

D

A

A

Q

Q

I

F

E

D

Q

A

G

A

L

M

K

K

I

I

I

L

A

Q

D

D

C

A

F

L

T

S

E

D

K267 (= K268) mutation to A: No GABA-AT activity.

3q8nC Crystal structure of 4-aminobutyrate transaminase from mycobacterium smegmatis (see paper)
45% identity, 99% coverage: 4:425/427 of query aligns to 17:439/439 of 3q8nC

query
sites
3q8nC

N

S

K

Q

A

E

M

L

M

Q

A

A

R

R

R

K

S

Q

D

S

A

A

V

V

P

A

R

A

G

G

V
|
V

G

G

Q

V

I

T

H

L

P

P

I

V

F

Y

A

V

E

V

R

A

A

A

E

G

N

G

C

G

R

V

V

L

W

A

D

D

V

A

E

D

G

G

R

N

E

Q

Y

L

L

I

D

D

F

F

A

G

G

S

G

G

I

I

A

A

V

V

L

T

N

T

T

V

G

G

H

N

L

S

H

A

P

P

Q

A

V

V

A

D

A

A

V

V

E

T

D

Q

Q

Q

L

V

K

A

L

F

S

T

H

H

T

T

C

C

F

F

Q

M

V

V

L

T

A

P

Y

Y

E

E

P

G

Y

Y

L

V

A

K

L

V

C

A

E

E

K

H

M

L

N

N

Q

R

K

L

V

T

P

P

G

G

D

D

F

H

A

E

K

K

K

R

T

T

L

A

L

L

V

F

T

N

T

S

G
|
G

S
x
A

E

E

A

A

V

V

E

E

N

N

A

A

V

V

K

K

I

I

A

A

R

R

A

A

A

Y

T

T

G

R

R

R

S

Q

G

A

A

V

I

V

A

V

F

F

T

D

G

H

A

A

Y
|
Y

H

H

G

G

R

R

T

T

H

N

Y

L

T

T

L

M

S

A

L

M

T

T

G

A

K

K

V

N

N

Q

P

P

Y

Y

S

K

A

H

G

G

M

F

G

G

L

P

M

F

P

A

G

N

H

E

V

V

Y

Y

R

R

-

V

-

P

A

T

L

S

Y

Y

P

P

C

F

A

R

L

D

H

G

G

E

V

T

S

D

D

G

D

A

E

A

A

A

I

A

A

A

S

H

I

A

H

L

R

D

I

L

F

I

K

N

N

K

D

Q

A

V

A

G

P

A

E

D

D

N

I

V

A

A

I

V

I

I

E
|
E

P

P

V

V

Q

H

G

G

E

E

G

G

F

F

Y

V

A

V

A

P

S

A

P

P

A

G

F

F

M

L

Q

G

R

A

L

L

R

Q

A

K

L

W

C

C

D

T

E

D

H

N

G

G

I

A

M

V

L

F

I

V

A

A

D
|
D

E

E

V
|
V

Q
|
Q

S

T

G

G

A

F

G

A

R

R

T

T

G

G

T

A

L

L

F

F

A

A

M

C

E

E

Q

H

M

E

G

N

V

V

A

V

A

P

D

D

I

L

T

I

T

V

F

T

A

A

K
|
K

S

G

I

I

A

A

G

G

F

L

P

P

L

L

A

S

G

A

V

V

T

T

G

G

R

R

A

A

E

E

V

I

M

M

D

D

A

G

I

P

A

Q

P

S

G

G

L

L

G

G

T
|
T

Y

Y

A

G

G

G

N

N

P

P

I

L

A

A

C

C

A

A

L

L

A

A

V

V

L

I

Q

D

I

T

F

I

E

E

Q

R

E

E

N

N

L

L

L

V

E

A

K

R

A

A

N

R

Q

A

L

I

G

G

D

E

T

T

L

M

R

L

Q

S

G

R

L

L

L

G

A

A

I

L

A

A

E

A

D

A

H

D

P

P

E

R

I

I

G

G

D

E

V

V

R

R

G

G

L

R

G

G

A

A

M

M

I

I

A

A

I

V

E

E

L

L

F

V

E

K

E

P

G

G

D

T

R

-

S

T

K

E

P

P

N

D

A

A

R

D

L

L

T

T

A

K

D

R

I

V

V

A

A

A

R

A

A

A

R

H

D

A

K

Q

G

G

L

L

I

V

L

V

L

L

S

T

C

C

G

G

P

T

Y

Y

Y

G

N

N

V

V

L

L

R
|
R

I

F

L

L

V

P

P

P

L

L

T

S

I

M

E

P

E

D

A

H

Q

L

I

L

E

D

Q

E

G

G

L

L

K

D

I

I

I

L

A

A

D

A

C

V

F

F

T

A

E

E

A

V

K

K

active site: V32 (= V19), Y151 (= Y138), E221 (= E206), D254 (= D239), Q257 (= Q242), K283 (= K268), T312 (= T297), R412 (= R398)
binding 4-oxobutanoic acid: G124 (= G111), A125 (≠ S112), V256 (= V241), K283 (= K268)

6j2vA Gaba aminotransferase from corynebacterium glutamicum (see paper)
44% identity, 97% coverage: 4:417/427 of query aligns to 20:431/440 of 6j2vA

query
sites
6j2vA

N

S

K

K

A

A

M

L

M

D

A

E

R

R

S

Q

D

A

A

A

V

V

P

A

R

R

G

A

V
x
L

G

A

Q

P

I

G

H

L

P

P

I

G

F

Y

A

V

E

V

R

D

A

A

E

D

N

G

C

G

R

I

V

L

W

A

D

D

V

A

E

D

G

G

R

N

E

R

Y

F

L

I

D

D

F

L

A

A

G

S

G

G

I

I

A

A

V

V

L

T

N

T

T

V

G

G

H

G

L

S

H

N

P

A

Q

A

V

V

V

A

A

K

A

A

V

V

E

G

D

A

Q

A

L

A

K

A

K

R

L

F

S

T

H

H

T

T

C

C

F

F

Q

M

V

V

L

S

A

P

Y

Y

E

E

P

T

Y

Y

L

V

A

A

L

M

C

A

E

E

K

R

M

L

N

N

Q

A

K

L

V

T

P

P

G

G

D

D

F

H

A

D

K

K

T

S

L

A

L

L

V

F

T

N

T

S

G
|
G

S
x
A

E

E

A

A

V

V

E

E

N

N

A

A

V

V

K

K

I

V

A

A

R

R

A

A

A

Y

T

T

G

G

R

K

S

G

G

A

A

V

I

V

A

V

F

F

T

D

G

N

A

A

Y
|
Y

H
|
H

G

G

R
|
R

T

T

H

N

Y

L

T

T

L

M

S

A

L

M

T

T

G

A

K

K

V

N

N

R

P

P

Y

Y

S

K

A

S

G

G

M

F

G

G

L

P

M

L

P

A

G

A

H

D

V

V

Y

Y

R

R

A

A

L

P

Y

M

P

S

C

Y

A

P

L

L

H

R

-

D

G

G

V

L

S

S

D

G

D

P

E

E

A

A

I

A

A

E

S

R

I

A

H

I

R

S

I

V

F

I

K

E

N

S

D

Q

A

V

A

G

P

A

E

E

D

N

I

L

A

A

A

C

I

V

I

I

E
|
E

P

P

V

I

Q

Q

G

G

E
|
E

G

G

F

F

Y

I

A

V

A

P

S

A

P

P

A

G

F

F

M

L

Q

A

R

A

L

I

R

S

A

T

L

W

C

C

D

R

E

E

H

N

G

D

I

V

M

V

L

F

I

I

A

A

D
|
D

E

E

V
x
I

Q

Q

S

S

G

G

A

F

G

L

R

R

T

T

G

G

T

D

L

W

F

F

A

A

M

S

E

D

Q

A

M

E

G

G

V

V

A

I

A

P

D

D

I

V

T

I

T

T

F

T

A

A

K
|
K

S

G

I

I

A

A

G

G

F

M

P

P

L

L

A

S

G

A

V

V

T

T

G

G

R

R

A

A

E

E

V

I

M

M

D

D

A

A

I

P

A

G

P

P

G

G

G

A

L

L

G

G

G
|
G

T
|
T

Y

Y

A

G

G

G

N

N

P

P

I

V

A

A

C

C

A

A

L

L

A

A

V

A

L

I

Q

E

I

V

F

M

E

E

Q

Q

E

A

N

D

L

L

L

K

E

T

K

R

A

A

N

Q

Q

E

L

I

G

E

D

T

T

I

L

I

R

R

Q

D

G

E

L

F

L

A

A

Q

I

L

A

S

E

A

D

-

H

F

P

P

E

E

I

V

G

A

D

E

V

I

R

R

G

G

L

R

G

G

A

A

M

M

I

M

A

A

I

I

E

E

L

L

F

I

E

D

E

A

G

T

D

G

R

R

S

-

K

-

P

P

N

N

A

A

R

A

L

L

T

T

A

A

D

A

I

V

V

A

A

A

R

R

A

A

R

K

D

A

K

E

G

G

L

V

I

L

L

L

S

T

C

C

G

G

P

T

Y

D

Y

G

N

N

V

V

L

I

R

R

I

L

L

L

V

P

P

P

L

L

T

V

I

I

E

A

E

E

A

D

Q

T

I

L

E

R

Q

D

G

G

L

L

K

Q

I

V

I

L

active site: L35 (≠ V19), Y154 (= Y138), D256 (= D239), K285 (= K268)
binding 4-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]butanoic acid: G127 (= G111), A128 (≠ S112), Y154 (= Y138), H155 (= H139), R157 (= R141), E223 (= E206), E228 (= E211), D256 (= D239), I258 (≠ V241), K285 (= K268), G313 (= G296), T314 (= T297)

4atqF Gaba-transaminase a1r958 in complex with external aldimine plp-gaba adduct (see paper)
45% identity, 97% coverage: 3:418/427 of query aligns to 19:438/444 of 4atqF

query
sites
4atqF

S

K

N

S

K

Q

A

A

M

L

M

A

A

E

R

R

S

S

D

A

A

V

V

V

P

A

R

A

G

G

V
|
V

G

A

Q

S

I

G

H

V

P

P

I

V

F

Y

A

V

E

E

R

D

A

A

E

D

N

G

C

G

R

I

V

I

W

R

D

D

V

V

E

D

G

G

R

N

E

S

Y

F

L

I

D

D

F

L

A

G

G

S

G

G

I

I

A

A

V

V

L

T

N

S

T

V

G

G

H

A

L

S

H

D

P

P

Q

A

V

V

A

A

V

V

E

Q

D

E

Q

A

L

A

K

A

K

H

L

F

S

T

H

H

T

T

C

C

F

F

Q
x
M

V

V

L

T

A

P

Y

Y

E

E

P

G

Y

Y

L

V

A

A

L

V

C

T

E

E

K

Q

M

L

N

N

Q

R

K

L

V

T

P

P

G

G

D

D

F

H

A

A

K

K

K

R

T

T

L

V

L

L

V

F

T

N

T

S

G
|
G

S
x
A

E

E

A

A

V

V

E

E

N

N

A

A

V

V

K

K

I

V

A

A

R

R

A

L

A

A

T

T

G

G

R

R

S

D

G

A

A

V

I

V

A

A

F

F

T

D

G

H

A

A

Y
|
Y

H
|
H

G

G

R
|
R

T

T

H

N

Y

L

T

T

L

M

S

A

L

L

T

T

G

A

K

K

V

A

N

M

P

P

Y

Y

S

K

A

T

G

N

M

F

G

G

L

P

M

F

P

A

G

P

H

E

V

V

Y

Y

R

R

-

M

-

P

A

M

L

S

Y

Y

P

P

C

F

A

R

L

E

H

E

G

N

-

P

-

E

V

I

S

T

D

G

D

A

E

E

A

A

I

A

A

K

S

R

I

A

H

I

R

T

I

M

F

I

K

E

N

K

D

Q

A

I

A

G

P

G

E

D

D

Q

I

V

A

A

I

I

E
|
E

P

P

V

I

Q

Q

G

G

E
|
E

G

G

F

F

Y

I

A

V

A

P

S

A

P

E

A

G

F

F

M

L

Q

P

R

A

L

L

R

S

A

E

L

W

C

A

D

K

E

E

H

K

G

G

I

I

M

V

L

F

I

I

A

A

D
|
D

E

E

V
|
V

Q
|
Q

S

S

G

G

A

F

G

C

R

R

T

T

G

G

T

E

L

W

F

F

A

A

M

V

E

D

Q

H

M

E

G

G

V

V

A

V

A

P

D

D

I

I

T

I

T

T

F

M

A

A

K
|
K

S

G

I

I

A

A

G

G

F

L

P

P

L

L

A

S

G

A

V

I

T

T

G

G

R

R

A

A

E

D

V

L

M

L

D

D

A

A

I

V

A

H

P

P

G

G

L

L

G

G

G
|
G

T
|
T

Y

Y

A

G

G

G

N

N

P

P

I

V

A

A

C

C

A

A

L

L

A

A

V

A

L

I

Q

D

I

T

F

M

E

E

Q

Q

E

H

N

D

L

L

L

N

E

G

K

R

A

A

N

R

Q

H

L

I

G

E

D

E

T

L

L

A

R

L

Q

G

G

K

L

L

L

R

A

E

I

L

-

A

A

A

E

E

D

G

H

G

P

S

E

V

I

V

G

G

D

D

V

I

R

R

G

G

L

R

G

G

A

A

M

M

I

L

A

A

I

I

E

E

L

L

F

V

E

Q

E

P

G

G

D

S

R

K

S

-

K

E

P

P

N

N

A

A

R

E

L

L

T

T

A

K

D

A

I

V

V

A

A

A

R

A

A

C

R

L

D

K

K

E

G

G

L

V

I

I

L

I

L

L

S

T

C

C

G

G

P

T

Y

Y

Y

G

N

N

V

V

L

I

R
|
R

I

L

L

L

V

P

P

P

L

L

T

V

I

I

E

S

E

D

A

E

Q

L

I

L

E

I

Q

D

G

G

L

L

K

E

I

V

I

L

A

A

active site: V35 (= V19), Y154 (= Y138), E226 (= E206), D259 (= D239), Q262 (= Q242), K288 (= K268), T317 (= T297), R418 (= R398)
binding gamma-amino-butanoic acid: M95 (≠ Q79), Y154 (= Y138), R157 (= R141), E231 (= E211), K288 (= K268), G316 (= G296)
binding pyridoxal-5'-phosphate: G127 (= G111), A128 (≠ S112), Y154 (= Y138), H155 (= H139), D259 (= D239), V261 (= V241)

O58478 Alanine/serine racemase; ASR; Ala/Ser racemase; EC 5.1.1.-; EC 5.1.1.1 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see paper)
35% identity, 97% coverage: 5:417/427 of query aligns to 33:454/474 of O58478

query
sites
O58478

K

K

A

E

M

L

M

I

A

E

R

R

R

E

S

K

D

K

A

V

V

L

P

S

R

T

G

G

V

I

G

G

-

V

Q

K

I

L

H

F

P

P

I

L

F

V

A

P

E

K

R

R

A

G

E

F

N

G

C

P

R

F

V

I

W

E

D

D

V

V

E

D

G

G

R

N

E

V

Y

F

L

I

D

D

F

F

A

L

G

A

G

G

I

A

A

A

V

A

L

A

N

S

T

T

G

G

H

Y

L

A

H

H

P

P

Q

K

V

L

V

V

A

K

A

A

V

V

E

K

D

E

Q

Q

L

V

K

E

K

L

L

I

S

Q

H

H

T

S

C

M

F

I

Q

G

V

Y

L

T

A

H

Y

S

E

E

P

R

Y

A

L

I

A

R

L

V

C

A

E

E

K

K

M

L

N

V

Q

E

K

I

V

S

P

P

G

I

D

E

F

-

A

N

K

S

K

K

T

V

L

I

L

F

V

G

T

L

T

S

G

G

S

S

E

D

A

A

V

V

E

D

N

M

A

A

V

I

K

K

I

V

A

S

R

K

A

F

A

S

T

T

G

R

R

R

S

P

G

W

A

I

I

L

A

A

F

F

T

I

G

G

A

A

Y

Y

H

H

G

G

R

Q

T

T

H

L

Y

G

T

A

L

T

S

S

L

V

T

A

G

S

-

F

K

Q

V

V

N

S

P

Q

Y

K

S

R

A

G

G

Y

M

S

G

P

L

L

M

M

P

P

G

-

H

N

V

V

Y

F

R

W

A

I

L

P

Y

Y

P

P

C

N

A

P

L

F

H

R

G

N

V

I

-

W

-

G

-

I

-

N

-

G

-

Y

-

E

S

E

D

P

D

D

E

E

A

L

I

I

A

N

S

R

I

V

-

L

-

D

-

Y

-

L

-

E

H

Y

R

Y

I

V

F

F

K

S

N

H

D

V

A

V

A

P

P

P

E

D

D

E

I

V

A

A

I

L

I

F

I

A

E

E

P

P

V

I

Q

Q

G

G

E
x
D

G

A

G

G

F

I

Y

V

A

V

A

P

S

P

P

E

A

N

F

F

M

F

Q

K

R

E

L

L

R

K

A

K

L

L

C

L

D

E

E

E

H

Y

G

G

I

I

M

L

L

L

I

V

A

M

D

D

E

E

V

V

Q

Q

S

T

G

G

A

I

G

G

R

R

T

T

G

G

T

K

L

W

F

F

A

A

M

S

E

E

Q

W

M

F

G

N

V

V

A

K

A

P

D

D

I

M

T

I

F

F

A

G

K
|
K

S

G

I

V

A

A

G

S

G

G

F

M

P

G

L

L

A

S

G

G

V

V

T

I

G

G

R

R

A

K

E

E

V

I

M

M

D

D

A

-

I

I

A

T

P

S

G

G

G

S

L

A

G

L

T

T

Y

P

A

A

G

A

N

N

P

P

I

V

A

I

C

S

A

A

L

E

A

A

V

T

L

L

Q

E

I

I

F

I

E

E

Q

E

E

E

N

N

L

L

E

K

K

N

A

A

N

L

Q

E

L

V

G

G

D

E

T

F

L

I

R

M

Q

G

G

R

L

L

L

K

A

E

I

I

A

K

E

E

D

R

H

F

P

E

E

I

I

I

G

G

D

D

V

V

R

R

G

G

L

K

G

G

A

L

M

M

I

I

A

G

I

V

E

E

L

I

F

V

E

K

E

E

G

N

D

G

R

R

S

-

K

-

P

P

N

D

A

P

R

E

L

M

T

T

A

G

D

K

I

I

V

C

A

W

R

R

A

A

R

F

D

E

K

L

G

G

L

L

I

I

L

L

L

P

S

S

C

Y

G

G

P

M

Y

F

Y

G

N

N

V

V

L

I

R

R

I

I

L

T

V

P

P

P

L

L

T

V

I

L

E

T

E

K

A

E

Q

V

I

A

E

E

Q

K

G

A

L

L

K

E

I

I

D251 (≠ E211) mutation to A: Loss of activity.
K308 (= K268) mutation to A: Loss of activity.

P40732 Acetylornithine/succinyldiaminopimelate aminotransferase; ACOAT; DapATase; Succinyldiaminopimelate transferase; EC 2.6.1.11; EC 2.6.1.17 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
37% identity, 96% coverage: 8:418/427 of query aligns to 1:397/405 of P40732

query
sites
P40732

M

M

A

A

R

T

R

E

S

Q

D

T

A

A

V

I

P

T

R

R

G

A

V

T

G

F

Q

D

-

E

-

V

I

I

H

L

P

P

I

V

F

Y

A

A

E

P

-

A

-

D

-

F

-

I

-

P

-

V

R

K

A

G

E

K

N

G

C

S

R

R

V

V

W

W

D

D

V

Q

E

Q

G

G

R

K

E

E

Y

Y

L

I

D

D

F

F

A

A

G

G

I

I

A

A

V

V

L

T

N

A

T

L

G

G

H

H

L

C

H

H

P

P

Q

A

V

L

V

V

A

E

A

A

V

L

E

K

D

S

Q

Q

L

G

K

E

K

T

L

L

S

W

H

H

T

T

C

S

F

-

Q

N

V

V

L

F

A

T

Y

N

E

E

P

P

Y

A

L

L

A

R

L

L

C

G

E

R

K

K

M

L

N

-

Q

-

K

-

V

I

P

D

G

A

D

T

F

F

A

A

K

E

K

R

T

V

L

L

L

F

V

M

T

N

T

S

G
|
G

S
x
T

E

E

A

A

V

N

E

E

N

T

A

A

V

F

K

K

I

L

A

A

R

R

A

H

-

Y

-

A

-

C

-

V

-

R

-

H

A

S

T

P

G

F

R

K

S

T

G

K

A

I

I

I

A

A

F

F

T

H

G

N

A

A

Y

F

H

H

G

G

R

R

T

S

H

L

Y

F

T

T

L

V

S

S

L

V

T

G

G

G

K

Q

V

P

N

K

P

-

Y

Y

S

S

A

D

G

G

M

F

G

G

L

P

M

K

P

P

G

A

H

D

V

I

Y

I

R

H

A

V

L

P

Y

F

P

-

C

N

A

D

L

L

H

H

G

A

V

V

S

-

D

-

E

K

A

A

I

V

A

M

S

D

I

D

H

H

R

-

I

-

F

-

K

-

N

-

D

-

A

-

P

-

E

-

D

-

I

T

A

C

A

A

I

V

E

E

P

P

V

I

Q

Q

G

G

E

E

G

G

F

V

Y

Q

A

A

S

T

P

P

A

E

F

F

M

L

Q

K

R

G

L

L

R

R

A

D

L

L

C

C

D

D

E

E

H

H

G

Q

I

A

M

L

L

L

I

V

A

F

D

D

E

E

V

V

Q

Q

S

C

G

G

A

M

G

G

R

R

T

T

G

G

T

D

L

L

F

F

A

A

M

Y

E

M

Q

H

M

Y

G

G

V

V

A

T

A

P

D

D

I

I

T

L

T

T

F

S

A

A

K
|
K

S

A

I

L

A

G

G

G

F

F

P

P

L

V

A

S

G

A

V

M

T

L

G

T

R

T

A

Q

E

E

V

I

M

A

D

S

A

A

I

F

A

H

P

V

G

G

G

S

L

H

G

G

G

S

T
|
T

Y

Y

A

G

G

G

N

N

P

P

I

L

A

A

C

C

A

A

A

V

A

A

L

G

A

A

V

A

L

F

Q

D

I

I

F

I

E

N

Q

T

E

P

N

E

L

V

L

L

E

Q

K

G

A

I

N

H

Q

T

L

K

G

R

D

Q

T

Q

L

F

R

V

Q

Q

G

H

L

L

L

Q

A

A

I

I

A

D

E

E

D

Q

H

F

P

D

E

I

I

F

G

S

D

D

V

I

R

R

G

G

L

M

G

G

A

L

M

L

I

I

A

G

I

A

E

E

L

L

F

-

E

-

G

-

D

-

R

-

S

-

K

K

P

P

N

K

A

Y

R

K

L

G

T

R

A

A

-

R

D

D

I

F

V

L

A

Y

R

A

A

G

R

A

D

E

K

A

G

G

L

V

I

M

L

V

L

L

S

N

C

A

G

G

P

A

Y

-

N

D

V

V

L

M

R

R

I

F

L

A

V

P

P

S

L

L

T

V

I

V

E

E

A

A

Q

D

I

I

E

H

Q

E

G

G

L

M

K

Q

I

R

I

F

A

A

GT 108:109 (≠ GS 111:112) binding pyridoxal 5'-phosphate
K255 (= K268) modified: N6-(pyridoxal phosphate)lysine
T284 (= T297) binding pyridoxal 5'-phosphate

4jevB N-acetylornithine aminotransferase from s. Typhimurium complexed with gabaculine
38% identity, 91% coverage: 29:418/427 of query aligns to 25:392/402 of 4jevB

query
sites
4jevB

R

K

A

G

E

K

N

G

C

S

R

R

V

V

W

W

D

D

V

Q

E

Q

G

G

R

K

E

E

Y

Y

L

I

D

D

F

F

A

A

G

G

I
|
I

A

A

V

V

L

T

N

A

T

L

G

G

H

H

L

C

H

H

P

P

Q

A

V

L

V

V

A

E

A

A

V

L

E

K

D

S

Q

Q

L

G

K

E

K

T

L

L

S

W

H

H

T

T

C

S

F

-

Q

N

V

V

L

F

A

T

Y

N

E

E

P

P

Y

A

L

L

A

R

L

L

C

G

E

R

K

K

M

L

N

-

Q

-

K

-

V

I

P

D

G

A

D

T

F

F

A

A

K

E

K

R

T

V

L

L

L

F

V

M

T

N

T
x
S

G
|
G

S
x
T

E

E

A

A

V

N

E

E

N

T

A

A

V

F

K

K

I

L

A

A

R

R

A

H

-

Y

-

A

-

C

-

V

-

R

-

H

A

S

T

P

G

F

R

K

S

T

G

K

A

I

I

I

A

A

F

F

T

H

G

N

A

A

Y
x
F

H
|
H

G

G

R

R

T

S

H

L

Y

F

T

T

L

V

S

S

L

V

T

G

G

G

K

Q

V

P

N

K

P

-

Y

Y

S

S

A

D

G

G

M

F

G

G

L

P

M

K

P

P

G

A

H

D

V

I

Y

I

R

H

A

V

L

P

Y

F

P

-

C

N

A

D

L

L

H

H

G

A

V

V

S

-

D

-

E

K

A

A

I

V

A

M

S

D

I

D

H

H

R

-

I

-

F

-

K

-

N

-

D

-

A

-

P

-

E

-

D

-

I

T

A

C

A

A

I

V

E
|
E

P

P

V

I

Q

Q

G

G

E
|
E

G

G

F

V

Y

Q

A

A

S

T

P

P

A

E

F

F

M

L

Q

K

R

G

L

L

R

R

A

D

L

L

C

C

D

D

E

E

H

H

G

Q

I

A

M

L

L

L

I

V

A

F

D
|
D

E

E

V
|
V

Q
|
Q

S

C

G

G

A

M

G

G

R

R

T

T

G

G

T

D

L

L

F

F

A

A

M

Y

E

M

Q

H

M

Y

G

G

V

V

A

T

A

P

D

D

I

I

T

L

T

T

F

S

A

A

K
|
K

S

A

I

L

A

G

G

G

F

F

P

P

L

V

A

S

G

A

V

M

T

L

G

T

R

T

A

Q

E

E

V

I

M

A

D

S

A

A

I

F

A

H

P

V

G

G

G

S

L

H

G

G

G

S

T
|
T

Y

Y

A

G

G

G

N

N

P

P

I

L

A

A

C

C

A

A

A

V

A

A

L

G

A

A

V

T

L

F

Q

D

I

I

F

I

E

N

Q

T

E

P

N

E

L

V

L

L

E

Q

K

G

A

I

N

H

Q

T

L

K

G

R

D

Q

T

Q

L

F

R

V

Q

Q

G

H

L

L

L

Q

A

A

I

I

A

D

E

E

D

Q

H

F

P

D

E

I

I

F

G

S

D

D

V

I

R

R

G

G

L

M

G

G

A

L

M

L

I

I

A

G

I

A

E

E

L

L

F

-

E

-

G

-

D

-

R

-

S

-

K

K

P

P

N

K

A

Y

R

K

L

G

T

R

A

A

-

R

D

D

I

F

V

L

A

Y

R

A

A

G

R

A

D

E

K

A

G

G

L

V

I

M

L

V

L

L

S

N

C

A

G

G

P

A

Y

-

N

D

V

V

L

M

R
|
R

I

F

L

A

V

P

P

S

L

L

T

V

I

V

E

E

A

A

Q

D

I

I

E

H

Q

E

G

G

L

M

K

Q

I

R

I

F

A

A

active site: F136 (≠ Y138), E188 (= E206), D221 (= D239), Q224 (= Q242), K250 (= K268), T279 (= T297), R372 (= R398)
binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: I46 (= I50), S102 (≠ T110), G103 (= G111), T104 (≠ S112), F136 (≠ Y138), H137 (= H139), E188 (= E206), E193 (= E211), D221 (= D239), V223 (= V241), Q224 (= Q242), K250 (= K268), R372 (= R398)

O50131 Ornithine aminotransferase; Orn-AT; Ornithine delta-aminotransferase; EC 2.6.1.13 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see paper)
39% identity, 93% coverage: 28:424/427 of query aligns to 41:441/454 of O50131

query
sites
O50131

E

E

R

R

A

A

E

E

N

G

C

V

R

Y

V

W

W

I

D

D

V

V

E

D

G

G

R

N

E

V

Y

L

L

L

D

D

F

F

A

S

G

S

G

G

I

I

A

G

V

V

L

M

N

N

T

V

G

G

H

L

L

R

H

N

P

P

Q

K

V

V

V

I

A

E

A

A

V

I

E

K

D

K

Q

Q

L

L

K

D

K

L

L

V

S

L

H

H

T

A

C

A

F

-

Q

G

V
x
T

L
x
D

A

Y

Y

Y

E

N

P

P

Y

Y

-

Q

L

V

A

E

L

L

C

A

E

K

K

K

M

L

N

V

Q

E

K

I

V

A

P

P

G

G

D

D

F

I

A

E

K

R

K

K

T

V

L

F

L

L

V

S

T

N

T

S

G
|
G

S
x
T

E

E

A

A

V

N

E

E

N

A

A

A

V

L

K

K

I

I

A

A

R

K

A

W

A

S

T

T

G

N

R

R

S

K

G

M

A

F

I

I

A

A

F

F

T

I

G

G

A

A

Y

F

H

H

G

G

R

R

T

T

H

H

Y

G

T

T

L

M

S

S

L

L

T

T

-

A

G

S

K

K

V

P

N

V

P

Q

Y

R

S

S

A

R

G

M

M

F

G

P

L

T

M

M

P

P

G

G

-

V

H

H

V

V

-

P

Y

Y

R

P

A

N

L

P

Y

Y

-

R

-

N

P

P

C

W

A

G

L

I

H

D

G

G

V

Y

S

E

D

N

-

P

D

D

E

E

A

L

I

I

A

N

S

R

I

V

-

I

-

D

-

Y

-

I

-

E

H

E

R

Y

I

L

F

F

K

E

N

H

D

Y

A

V

A

P

P

A

E

E

D

E

I

V

A

A

A

G

I

I

I

F

E

E

P

P

V

I

Q

Q

G

G

E

E

G

G

F

Y

Y

V

A

V

A

P

S

P

P

K

A

N

F

F

M

F

Q

K

R

E

L

L

R

K

A

K

L

L

C

A

D

D

E

K

H

H

G

G

I

I

M

L

L

L

I

I

A

D

D

D

E

E

V

V

Q
|
Q

S

M

G

G

A

M

G

G

R

R

T

T

G

G

T

R

L

M

F

W

A

A

M

I

E

E

Q

H

M

F

G

D

V

I

A

V

A

P

D

D

I

I

T

V

T

T

F

V

A

A

K
|
K

S

A

I

L

A

G

G

G

F

I

P

P

L

I

A

G

G

A

V

T

T

I

G

F

R

R

A

A

E

D

V

-

M

L

D

D

A

F

I

G

A

V

P

S

G

G

G

V

L

H

G

S

G

N

T
|
T

Y

F

A

G

G

G

N

N

P

T

I

V

A

A

C

A

A

A

L

L

A

A

V

V

L

I

Q

E

I

E

F

L

E

-

Q

Q

E

N

N

G

L

L

L

I

E

E

K

N

A

A

N

Q

Q

K

L

L

G

E

D

P

T

L

L

F

R

R

Q

E

G

R

L

L

L

E

A

E

I

M

A

K

E

E

D

K

H

Y

P

E

E

I

I

I

G

G

D

D

V

V

R

R

G

G

L

L

G

G

A

L

M

A

I

W

A

G

I

V

E

E

L

F

F

V

E

K

E

D

G

R

D

K

R

T

S

K

K

E

P

Y

N

A

A

T

R

K

L

E

T

R

A

G

D

E

I

I

V

V

A

V

R

E

A

A

R

L

D

K

K

R

G

G

L

L

I

A

L

L

S

G

C

C

G

G

P

K

Y

-

N

S

V

A

L

I

R

R

I

L

L

I

V

P

P

P

L

L

T

I

I

I

E

S

E

E

A

E

Q

E

I

A

E

K

Q

M

G

G

L

L

K

-

I

-

A

-

D

D

C

I

F

F

T

E

E

E

A

A

T92 (≠ V80) mutation to V: Slightly increases the specific activity. Increases KM for L-ornithine.
D93 (≠ L81) mutation to L: Reduces the specific activity. Increases KM for L-ornithine.
G124 (= G111) binding pyridoxal 5'-phosphate
T125 (≠ S112) binding pyridoxal 5'-phosphate
Q267 (= Q242) binding pyridoxal 5'-phosphate
K293 (= K268) modified: N6-(pyridoxal phosphate)lysine
T321 (= T297) binding pyridoxal 5'-phosphate

7vo1A Structure of aminotransferase-substrate complex (see paper)
39% identity, 93% coverage: 28:424/427 of query aligns to 39:439/452 of 7vo1A

query
sites
7vo1A

E

E

R

R

A

A

E

E

N

G

C

V

R

Y

V

W

W

I

D

D

V

V

E

D

G

G

R

N

E

V

Y

L

L

L

D

D

F

F

A

S

G

S

G

G

I
|
I

A

G

V

V

L

M

N

N

T

V

G

G

H

L

L

R

H

N

P

P

Q

K

V

V

V

I

A

E

A

A

V

I

E

K

D

K

Q

Q

L

L

K

D

K

L

L

V

S

L

H

H

T

A

C

A

F

-

Q

G

V

T

L

D

A

Y

Y

Y

E

N

P

P

Y

Y

-

Q

L

V

A

E

L

L

C

A

E

K

K

K

M

L

N

V

Q

E

K

I

V

A

P

P

G

G

D

D

F

I

A

E

K

R

K

K

T

V

L

F

L

L

V

S

T

N

T
x
S

G
|
G

S
x
T

E

E

A

A

V

N

E

E

N

A

A

A

V

L

K

K

I

I

A

A

R

K

A

W

A

S

T

T

G

N

R

R

S

K

G

M

A

F

I

I

A

A

F

F

T

I

G

G

A

A

Y
x
F

H
|
H

G

G

R
|
R

T

T

H

H

Y

G

T

T

L

M

S

S

L

L

T

T

-

A

G

S

K

K

V

P

N

V

P

Q

Y

R

S

S

A

R

G

M

M

F

G

P

L

T

M

M

P

P

G

G

-

V

H

H

V

V

-

P

Y

Y

R

P

A

N

L

P

Y

Y

-

R

-

N

P

P

C

W

A

G

L

I

H

D

G

G

V

Y

S

E

D

N

-

P

D

D

E

E

A

L

I

I

A

N

S

R

I

V

-

I

-

D

-

Y

-

I

-

E

H

E

R

Y

I

L

F

F

K

E

N

H

D

Y

A

V

A

P

P

A

E

E

D

E

I

V

A

A

A

G

I

I

I

F

E

E

P

P

V

I

Q

Q

G

G

E
|
E

G

G

F

Y

Y

V

A

V

A

P

S

P

P

K

A

N

F

F

M

F

Q

K

R

E

L

L

R

K

A

K

L

L

C

A

D

D

E

K

H

H

G

G

I

I

M

L

L

L

I

I

A

D

D
|
D

E

E

V
|
V

Q
|
Q

S

M

G

G

A

M

G

G

R

R

T

T

G

G

T

R

L

M

F

W

A

A

M

I

E

E

Q

H

M

F

G

D

V

I

A

V

A

P

D

D

I

I

T

V

T

T

F

V

A

A

K
|
K

S

A

I

L

A

G

G

G

F

I

P

P

L

I

A

G

G

A

V

T

T

I

G

F

R

R

A

A

E

D

V

-

M

L

D

D

A

F

I

G

A

V

P

S

G

G

G

V

L

H

G

S

G
x
N

T
|
T

Y

F

A

G

G

G

N

N

P

T

I

V

A

A

C

A

A

A

L

L

A

A

V

V

L

I

Q

E

I

E

F

L

E

-

Q

Q

E

N

N

G

L

L

L

I

E

E

K

N

A

A

N

Q

Q

K

L

L

G

E

D

P

T

L

L

F

R

R

Q

E

G

R

L

L

L

E

A

E

I

M

A

K

E

E

D

K

H

Y

P

E

E

I

I

I

G

G

D

D

V

V

R

R

G

G

L

L

G

G

A

L

M

A

I

W

A

G

I

V

E

E

L

F

F

V

E

K

E

D

G

R

D

K

R

T

S

K

K

E

P

Y

N

A

A

T

R

K

L

E

T

R

A

G

D

E

I

I

V

V

A

V

R

E

A

A

R

L

D

K

K

R

G

G

L

L

I

A

L

L

S

G

C

C

G

G

P

K

Y

-

N

S

V

A

L

I

R
|
R

I

L

L

I

V

P

P

P

L

L

T

I

I

I

E

S

E

E

A

E

Q

E

I

A

E

K

Q

M

G

G

L

L

K

-

I

-

A

-

D

D

C

I

F

F

T

E

E

E

A

A

binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: I61 (= I50), S121 (≠ T110), G122 (= G111), T123 (≠ S112), F149 (≠ Y138), H150 (= H139), R152 (= R141), E234 (= E211), D262 (= D239), V264 (= V241), Q265 (= Q242), K291 (= K268), N318 (≠ G296), T319 (= T297), R417 (= R398)

7vntA Structure of aminotransferase-substrate complex (see paper)
39% identity, 93% coverage: 28:424/427 of query aligns to 39:439/452 of 7vntA

query
sites
7vntA

E

E

R

R

A

A

E

E

N

G

C

V

R

Y

V

W

W

I

D

D

V

V

E

D

G

G

R

N

E

V

Y

L

L

L

D

D

F

F

A

S

G

S

G

G

I

I

A

G

V

V

L

M

N

N

T

V

G

G

H

L

L

R

H

N

P

P

Q

K

V

V

V

I

A

E

A

A

V

I

E

K

D

K

Q

Q

L

L

K

D

K

L

L

V

S

L

H

H

T

A

C

A

F

-

Q

G

V

T

L

D

A

Y

Y

Y

E

N

P

P

Y

Y

-

Q

L

V

A

E

L

L

C

A

E

K

K

K

M

L

N

V

Q

E

K

I

V

A

P

P

G

G

D

D

F

I

A

E

K

R

K

K

T

V

L

F

L

L

V

S

T

N

T

S

G
|
G

S
x
T

E

E

A

A

V

N

E

E

N

A

A

A

V

L

K

K

I

I

A

A

R

K

A

W

A

S

T

T

G

N

R

R

S

K

G

M

A

F

I

I

A

A

F

F

T

I

G

G

A

A

Y
x
F

H
|
H

G

G

R
|
R

T

T

H

H

Y

G

T

T

L

M

S

S

L

L

T

T

-

A

G

S

K

K

V

P

N

V

P

Q

Y

R

S

S

A

R

G

M

M

F

G

P

L

T

M

M

P

P

G

G

-

V

H

H

V

V

-

P

Y

Y

R

P

A

N

L

P

Y

Y

-

R

-

N

P

P

C

W

A

G

L

I

H

D

G

G

V

Y

S

E

D

N

-

P

D

D

E

E

A

L

I

I

A

N

S

R

I

V

-

I

-

D

-

Y

-

I

-

E

H

E

R

Y

I

L

F

F

K

E

N

H

D

Y

A

V

A

P

P

A

E

E

D

E

I

V

A

A

A

G

I

I

I

F

E
|
E

P

P

V

I

Q

Q

G

G

E
|
E

G

G

F

Y

Y

V

A

V

A

P

S

P

P

K

A

N

F

F

M

F

Q

K

R

E

L

L

R

K

A

K

L

L

C

A

D

D

E

K

H

H

G

G

I

I

M

L

L

L

I

I

A

D

D
|
D

E

E

V
|
V

Q
|
Q

S

M

G

G

A

M

G

G

R

R

T

T

G

G

T

R

L

M

F

W

A

A

M

I

E

E

Q

H

M

F

G

D

V

I

A

V

A

P

D

D

I

I

T

V

T

T

F

V

A

A

K
|
K

S

A

I

L

A

G

G

G

F

I

P

P

L

I

A

G

G

A

V

T

T

I

G

F

R

R

A

A

E

D

V

-

M

L

D

D

A

F

I

G

A

V

P

S

G

G

G

V

L

H

G

S

G

N

T

T

Y

F

A

G

G

G

N

N

P

T

I

V

A

A

C

A

A

A

L

L

A

A

V

V

L

I

Q

E

I

E

F

L

E

-

Q

Q

E

N

N

G

L

L

L

I

E

E

K

N

A

A

N

Q

Q

K

L

L

G

E

D

P

T

L

L

F

R

R

Q

E

G

R

L

L

L

E

A

E

I

M

A

K

E

E

D

K

H

Y

P

E

E

I

I

I

G

G

D

D

V

V

R

R

G

G

L

L

G

G

A

L

M

A

I

W

A

G

I

V

E

E

L

F

F

V

E

K

E

D

G

R

D

K

R

T

S

K

K

E

P

Y

N

A

A

T

R

K

L

E

T

R

A

G

D

E

I

I

V

V

A

V

R

E

A

A

R

L

D

K

K

R

G

G

L

L

I

A

L

L

S

G

C

C

G

G

P

K

Y

-

N

S

V

A

L

I

R

R

I

L

L

I

V

P

P

P

L

L

T

I

I

I

E

S

E

E

A

E

Q

E

I

A

E

K

Q

M

G

G

L

L

K

-

I

-

A

-

D

D

C

I

F

F

T

E

E

E

A

A

binding L-ornithine: F149 (≠ Y138), R152 (= R141), E234 (= E211), K291 (= K268)
binding pyridoxal-5'-phosphate: G122 (= G111), T123 (≠ S112), F149 (≠ Y138), H150 (= H139), E229 (= E206), D262 (= D239), V264 (= V241), Q265 (= Q242), K291 (= K268)

7vnoA Structure of aminotransferase (see paper)
39% identity, 93% coverage: 28:424/427 of query aligns to 39:439/452 of 7vnoA

query
sites
7vnoA

E

E

R

R

A

A

E

E

N

G

C

V

R

Y

V

W

W

I

D

D

V

V

E

D

G

G

R

N

E

V

Y

L

L

L

D

D

F

F

A

S

G

S

G

G

I

I

A

G

V

V

L

M

N

N

T

V

G

G

H

L

L

R

H

N

P

P

Q

K

V

V

V

I

A

E

A

A

V

I

E

K

D

K

Q

Q

L

L

K

D

K

L

L

V

S

L

H

H

T

A

C

A

F

-

Q

G

V

T

L

D

A

Y

Y

Y

E

N

P

P

Y

Y

-

Q

L

V

A

E

L

L

C

A

E

K

K

K

M

L

N

V

Q

E

K

I

V

A

P

P

G

G

D

D

F

I

A

E

K

R

K

K

T

V

L

F

L

L

V

S

T

N

T

S

G
|
G

S
x
T

E

E

A

A

V
x
N

E

E

N

A

A

A

V

L

K

K

I

I

A

A

R

K

A

W

A

S

T

T

G

N

R

R

S

K

G

M

A

F

I

I

A

A

F

F

T

I

G

G

A

A

Y
x
F

H
|
H

G

G

R

R

T

T

H

H

Y

G

T

T

L

M

S

S

L

L

T

T

-

A

G

S

K

K

V

P

N

V

P

Q

Y

R

S

S

A

R

G

M

M

F

G

P

L

T

M

M

P

P

G

G

-

V

H

H

V

V

-

P

Y

Y

R

P

A

N

L

P

Y

Y

-

R

-

N

P

P

C

W

A

G

L

I

H

D

G

G

V

Y

S

E

D

N

-

P

D

D

E

E

A

L

I

I

A

N

S

R

I

V

-

I

-

D

-

Y

-

I

-

E

H

E

R

Y

I

L

F

F

K

E

N

H

D

Y

A

V

A

P

P

A

E

E

D

E

I

V

A

A

A

G

I

I

I

F

E
|
E

P

P

V

I

Q

Q

G

G

E

E

G

G

F

Y

Y

V

A

V

A

P

S

P

P

K

A

N

F

F

M

F

Q

K

R

E

L

L

R

K

A

K

L

L

C

A

D

D

E

K

H

H

G

G

I

I

M

L

L

L

I

I

A

D

D
|
D

E

E

V
|
V

Q
|
Q

S

M

G

G

A

M

G

G

R

R

T

T

G

G

T

R

L

M

F

W

A

A

M

I

E

E

Q

H

M

F

G

D

V

I

A

V

A

P

D

D

I

I

T

V

T

T

F

V

A

A

K
|
K

S

A

I

L

A

G

G

G

F

I

P

P

L

I

A

G

G

A

V

T

T

I

G

F

R

R

A

A

E

D

V

-

M

L

D

D

A

F

I

G

A

V

P

S

G

G

G

V

L

H

G

S

G

N

T

T

Y

F

A

G

G

G

N

N

P

T

I

V

A

A

C

A

A

A

L

L

A

A

V

V

L

I

Q

E

I

E

F

L

E

-

Q

Q

E

N

N

G

L

L

L

I

E

E

K

N

A

A

N

Q

Q

K

L

L

G

E

D

P

T

L

L

F

R

R

Q

E

G

R

L

L

L

E

A

E

I

M

A

K

E

E

D

K

H

Y

P

E

E

I

I

I

G

G

D

D

V

V

R

R

G

G

L

L

G

G

A

L

M

A

I

W

A

G

I

V

E

E

L

F

F

V

E

K

E

D

G

R

D

K

R

T

S

K

K

E

P

Y

N

A

A

T

R

K

L

E

T

R

A

G

D

E

I

I

V

V

A

V

R

E

A

A

R

L

D

K

K

R

G

G

L

L

I

A

L

L

S

G

C

C

G

G

P

K

Y

-

N

S

V

A

L

I

R

R

I

L

L

I

V

P

P

P

L

L

T

I

I

I

E

S

E

E

A

E

Q

E

I

A

E

K

Q

M

G

G

L

L

K

-

I

-

A

-

D

D

C

I

F

F

T

E

E

E

A

A

binding pyridoxal-5'-phosphate: G122 (= G111), T123 (≠ S112), N126 (≠ V115), F149 (≠ Y138), H150 (= H139), E229 (= E206), D262 (= D239), V264 (= V241), Q265 (= Q242), K291 (= K268)

5wyfA Structure of amino acid racemase, 2.12 a (see paper)
33% identity, 93% coverage: 28:422/427 of query aligns to 32:432/446 of 5wyfA

query
sites
5wyfA

E

D

R

H

A

A

E

H

N

G

C

A

R

T

V

L

W

V

D

D

V

V

E

D

G

G

R

N

E

K

Y

Y

L

I

D

D

F

L

A

L

G

A

G

S

I
x
A

A

S

V

A

L

I

N

N

T

V

G

G

H

H

L

T

H

H

P

E

Q

K

V

V

A

K

A

A

V

I

E

A

D

D

Q

Q

L

A

K

Q

K

K

L

L

S

I

H

H

T

Y

C

T

F

P

Q

A

V
x
Y

L

F

A

H

Y

H

E

V

P

P

Y

G

L

M

A

E

L

L

C

S

E

E

K

K

M

L

N

A

Q

K

K

I

V

A

P

P

G

G

D

N

F

S

A

P

K

K

K

M

T

V

L

S

L

F

V

G

T

N

T

S

G
|
G

S
|
S

E

D

A

A

V

N

E

D

N

A

A

I

V

I

K

K

I

F

A

A

R

R

A

A

A

Y

T

T

G

G

R

R

S

Q

G

Y

A

I

I

V

A

S

F

Y

T

M

G

G

A

S

Y
|
Y

H
|
H

G

G

R

S

T

T

H

Y

Y

G

T

S

L

Q

S

T

L

L

T

S

G

G

K

S

V

S

N

L

P

N

Y

M

S

T

A

R

G

K

M

I

G

G

L

P

M

M

P

L

G

P

H

S

V

V

Y

V

R

H

A

V

L

P

Y

Y

P

P

C

D

A

S

L

Y

H

R

G

T

V

Y

S

P

D

G

D

E

E

T

A

E

I

H

A

D

S

V

I

S

H

L

R

R

I

Y

F

F

K

N

N

E

D

F

A

K

P

P

-

F

-

E

-

S

-

F

-

L

-

P

-

A

E

D

D

E

I

T

A

A

A

C

I

V

I

L

I

I

E
|
E

P

P

V

I

Q

Q

G

G

E
x
D

G

G

F

I

Y

I

A

K

A

A

S

P

P

E

A

E

F

Y

M

M

Q

Q

R

L

L

V

R

Y

A

K

L

F

C

C

D

H

E

E

H

H

G

G

I

I

M

L

L

F

I

A

A

I

D
|
D

E

E

V
|
V

Q
x
N

S

Q

G

G

A

L

G

G

R

R

T

T

G

G

T

K

L

M

F

W

A

A

M

I

E

Q

Q

Q

M

F

-

K

G

D

V

I

A

E

A

P

D

D

I

L

T

M

T

S

F

V

A

G

K
|
K

S

S

I

L

A

A

G

S

G

G

F

M

P

P

L

L

A

S

G

A

V

V

T

I

G

G

R

K

A

K

E

E

V

V

M

M

D

Q

A

S

I

L

-

D

A

A

P

P

G

A

G

H

L

L

G
x
F

G
x
T

T

T

Y

-

A

A

G

G

N

N

P

P

I

V

A

C

C

S

A

A

A

S

L

L

A

A

V

T

L

L

Q

D

I

V

F

I

E

E

Q

Y

E

E

N

G

L

L

L

V

E

E

K

K

A

S

N

A

Q

T

L

D

G

G

D

A

T

Y

L

A

R

K

Q

Q

G

R

L

F

L

L

A

E

I

M

A

Q

E

Q

D

R

H

H

P

P

E

M

I

I

G

G

D

D

V

V

R

R

G

M

L

W

G

G

A

L

M

N

I

G

A

G

I

I

E

E

L

L

F

V

E

K

E

D

G

P

D

K

R

T

S

K

K

E

P

P

N

D

A

S

R

D

L

A

T

A

A

T

D

K

I

V

V

I

A

Y

R

Y

A

A

R

F

D

A

K

H

G

G

L

V

I

V

L

I

S

T

C

L

G

A

P

G

Y

-

N

N

V

I

L

L

R
|
R

I

F

L

Q

V

P

P

P

L

L

T

V

I

I

E

P

E

R

A

E

Q

Q

I

L

E

D

Q

Q

G

A

L

L

K

Q

I

V

I

L

A

D

D

D

C

A

F

F

T

T

active site: Y142 (= Y138), E217 (= E206), D250 (= D239), N253 (≠ Q242), K280 (= K268), T309 (≠ G296), R408 (= R398)
binding n-[o-phosphono-pyridoxyl]-isoleucine: A54 (≠ I50), Y84 (≠ V80), G115 (= G111), S116 (= S112), Y142 (= Y138), H143 (= H139), D222 (≠ E211), D250 (= D239), V252 (= V241), N253 (≠ Q242), K280 (= K268), F308 (≠ G295), T309 (≠ G296), R408 (= R398)

Sites not aligning to the query:

active site: 22

5wyaA Structure of amino acid racemase, 2.65 a (see paper)
33% identity, 93% coverage: 28:422/427 of query aligns to 30:430/439 of 5wyaA

query
sites
5wyaA

E

D

R

H

A

A

E

H

N

G

C

A

R

T

V

L

W

V

D

D

V

V

E

D

G

G

R

N

E

K

Y

Y

L

I

D

D

F

L

A

L

G

A

G

S

I
x
A

A

S

V

A

L

I

N

N

T

V

G

G

H

H

L

T

H

H

P

E

Q

K

V

V

A

K

A

A

V

I

E

A

D

D

Q

Q

L

A

K

Q

K

K

L

L

S

I

H

H

T

Y

C

T

F

P

Q

A

V
x
Y

L

F

A

H

Y

H

E

V

P

P

Y

G

L

M

A

E

L

L

C

S

E

E

K

K

M

L

N

A

Q

K

K

I

V

A

P

P

G

G

D

N

F

S

A

P

K

K

K

M

T

V

L

S

L

F

V

G

T

N

T
x
S

G
|
G

S
|
S

E

D

A

A

V

N

E

D

N

A

A

I

V

I

K

K

I

F

A

A

R

R

A

A

A

Y

T

T

G

G

R

R

S

Q

G

Y

A

I

I

V

A

S

F

Y

T

M

G

G

A

S

Y
|
Y

H
|
H

G

G

R

S

T

T

H

Y

Y

G

T

S

L

Q

S

T

L

L

T

S

G

G

K

S

V

S

N

L

P

N

Y

M

S

T

A

R

G

K

M

I

G

G

L

P

M

M

P

L

G

P

H

S

V

V

Y

V

R

H

A

V

L

P

Y

Y

P

P

C

D

A

S

L

Y

H

R

G

T

V

Y

S

P

D

G

D

E

E

T

A

E

I

H

A

D

S

V

I

S

H

L

R

R

I

Y

F

F

K

N

N

E

D

F

A

K

P

P

-

F

-

E

-

S

-

F

-

L

-

P

-

A

E

D

D

E

I

T

A

A

A

C

I

V

I

L

I

I

E
|
E

P

P

V

I

Q

Q

G

G

E

D

G

G

F

I

Y

I

A

K

A

A

S

P

P

E

A

E

F

Y

M

M

Q

Q

R

L

L

V

R

Y

A

K

L

F

C

C

D

H

E

E

H

H

G

G

I

I

M

L

L

F

I

A

A

I

D
|
D

E

E

V
|
V

Q
x
N

S

Q

G

G

A

L

G

G

R

R

T

T

G

G

T

K

L

M

F

W

A

A

M

I

E

Q

Q

Q

M

F

-

K

G

D

V

I

A

E

A

P

D

D

I

L

T

M

T

S

F

V

A

G

K
|
K

S

S

I

L

A

A

G

S

G

G

F

M

P

P

L

L

A

S

G

A

V

V

T

I

G

G

R

K

A

K

E

E

V

V

M

M

D

Q

A

S

I

L

-

D

A

A

P

P

G

A

G

H

L

L

G
x
F

G
x
T

T

T

Y

-

A

A

G

G

N

N

P

P

I

V

A

C

C

S

A

A

A

S

L

L

A

A

V

T

L

L

Q

D

I

V

F

I

E

E

Q

Y

E

E

N

G

L

L

L

V

E

E

K

K

A

S

N

A

Q

T

L

D

G

G

D

A

T

Y

L

A

R

K

Q

Q

G

R

L

F

L

L

A

E

I

M

A

Q

E

Q

D

R

H

H

P

P

E

M

I

I

G

G

D

D

V

V

R

R

G

M

L

W

G

G

A

L

M

N

I

G

A

G

I

I

E

E

L

L

F

V

E

K

E

D

G

P

D

K

R

T

S

K

K

E

P

P

N

D

A

S

R

D

L

A

T

A

A

T

D

K

I

V

V

I

A

Y

R

Y

A

A

R

F

D

A

K

H

G

G

L

V

I

V

L

I

S

T

C

L

G

A

P

G

Y

-

N

N

V

I

L

L

R
|
R

I

F

L

Q

V

P

P

P

L

L

T

V

I

I

E

P

E

R

A

E

Q

Q

I

L

E

D

Q

Q

G

A

L

L

K

Q

I

V

I

L

A

D

D

D

C

A

F

F

T

T

active site: Y140 (= Y138), E215 (= E206), D248 (= D239), N251 (≠ Q242), K278 (= K268), T307 (≠ G296), R406 (= R398)
binding (2S,3S)-3-methyl-2-[[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylamino]pentanoic acid: A52 (≠ I50), Y82 (≠ V80), S112 (≠ T110), G113 (= G111), S114 (= S112), Y140 (= Y138), H141 (= H139), E215 (= E206), D248 (= D239), V250 (= V241), N251 (≠ Q242), K278 (= K268), F306 (≠ G295), T307 (≠ G296), R406 (= R398)

Sites not aligning to the query:

active site: 20

4ysnC Structure of aminoacid racemase in complex with plp (see paper)
33% identity, 93% coverage: 28:422/427 of query aligns to 39:439/448 of 4ysnC

query
sites
4ysnC

E

D

R

H

A

A

E

H

N

G

C

A

R

T

V

L

W

V

D

D

V

V

E

D

G

G

R

N

E

K

Y

Y

L

I

D

D

F

L

A

L

G

A

G

S

I

A

A

S

V

A

L

I

N

N

T

V

G

G

H

H

L

T

H

H

P

E

Q

K

V

V

A

K

A

A

V

I

E

A

D

D

Q

Q

L

A

K

Q

K

K

L

L

S

I

H

H

T

Y

C

T

F

P

Q

A

V

Y

L

F

A

H

Y

H

E

V

P

P

Y

G

L

M

A

E

L

L

C

S

E

E

K

K

M

L

N

A

Q

K

K

I

V

A

P

P

G

G

D

N

F

S

A

P

K

K

K

M

T

V

L

S

L

F

V

G

T

N

T
x
S

G
|
G

S
|
S

E

D

A

A

V

N

E

D

N

A

A

I

V

I

K

K

I

F

A

A

R

R

A

A

A

Y

T

T

G

G

R

R

S

Q

G

Y

A

I

I

V

A

S

F

Y

T

M

G

G

A

S

Y
|
Y

H
|
H

G

G

R

S

T

T

H

Y

Y

G

T

S

L

Q

S

T

L

L

T

S

G

G

K

S

V

S

N

L

P

N

Y

M

S

T

A

R

G

K

M

I

G

G

L

P

M

M

P

L

G

P

H

S

V

V

Y

V

R

H

A

V

L

P

Y

Y

P

P

C

D

A

S

L

Y

H

R

G

T

V

Y

S

P

D

G

D

E

E

T

A

E

I

H

A

D

S

V

I

S

H

L

R

R

I

Y

F

F

K

N

N

E

D

F

A

K

P

P

-

F

-

E

-

S

-

F

-

L

-

P

-

A

E

D

D

E

I

T

A

A

A

C

I

V

I

L

I

I

E
|
E

P

P

V

I

Q

Q

G

G

E

D

G

G

F

I

Y

I

A

K

A

A

S

P

P

E

A

E

F

Y

M

M

Q

Q

R

L

L

V

R

Y

A

K

L

F

C

C

D

H

E

E

H

H

G

G

I

I

M

L

L

F

I

A

A

I

D
|
D

E

E

V
|
V

Q
x
N

S

Q

G

G

A

L

G

G

R

R

T

T

G

G

T

K

L

M

F

W

A

A

M

I

E

Q

Q

Q

M

F

-

K

G

D

V

I

A

E

A

P

D

D

I

L

T

M

T

S

F

V

A

G

K
|
K

S

S

I

L

A

A

G

S

G

G

F

M

P

P

L

L

A

S

G

A

V

V

T

I

G

G

R

K

A

K

E

E

V

V

M

M

D

Q

A

S

I

L

-

D

A

A

P

P

G

A

G

H

L

L

G
x
F

G
x
T

T

T

Y

-

A

A

G

G

N

N

P

P

I

V

A

C

C

S

A

A

A

S

L

L

A

A

V

T

L

L

Q

D

I

V

F

I

E

E

Q

Y

E

E

N

G

L

L

L

V

E

E

K

K

A

S

N

A

Q

T

L

D

G

G

D

A

T

Y

L

A

R

K

Q

Q

G

R

L

F

L

L

A

E

I

M

A

Q

E

Q

D

R

H

H

P

P

E

M

I

I

G

G

D

D

V

V

R

R

G

M

L

W

G

G

A

L

M

N

I

G

A

G

I

I

E

E

L

L

F

V

E

K

E

D

G

P

D

K

R

T

S

K

K

E

P

P

N

D

A

S

R

D

L

A

T

A

A

T

D

K

I

V

V

I

A

Y

R

Y

A

A

R

F

D

A

K

H

G

G

L

V

I

V

L

I

S

T

C

L

G

A

P

G

Y

-

N

N

V

I

L

L

R
|
R

I

F

L

Q

V

P

P

P

L

L

T

V

I

I

E

P

E

R

A

E

Q

Q

I

L

E

D

Q

Q

G

A

L

L

K

Q

I

V

I

L

A

D

D

D

C

A

F

F

T

T

active site: Y149 (= Y138), E224 (= E206), D257 (= D239), N260 (≠ Q242), K287 (= K268), T316 (≠ G296), R415 (= R398)
binding pyridoxal-5'-phosphate: S121 (≠ T110), G122 (= G111), S123 (= S112), Y149 (= Y138), H150 (= H139), E224 (= E206), D257 (= D239), V259 (= V241), K287 (= K268), F315 (≠ G295), T316 (≠ G296)

Sites not aligning to the query:

active site: 29

4jewA N-acetylornithine aminotransferase from s. Typhimurium complexed with l-canaline
37% identity, 93% coverage: 22:418/427 of query aligns to 12:387/397 of 4jewA

query
sites
4jewA

I

I

H

L

P

P

I

V

F

Y

A

A

E

P

-

A

-

D

-

F

-

I

-

P

-

V

R
x
K

A

G

E
x
K

N

G

C

S

R

R

V

V

W
|
W

D

D

V

Q

E

Q

G

G

R

K

E

E

Y

Y

L

I

D

D

F

F

A

A

G

G

I

I

A

A

V

V

L

T

N

A

T

L

G

G

H

H

L

C

H

H

P

P

Q

A

V

L

V

V

A

E

A

A

V

L

E

K

D

S

Q

Q

L

G

K

E

K

T

L

L

S

W

H

H

T

T

C

S

F

-

Q

N

V

V

L

F

A

T

Y

N

E

E

P

P

Y

A

L

L

A

R

L

L

C

G

E

R

K

K

M

L

N

-

Q

-

K

-

V

I

P

D

G

A

D

T

F

F

A

A

K

E

K

R

T

V

L

L

L

F

V

M

T

N

T

S

G
|
G

S
x
T

E

E

A

A

V

N

E

E

N

T

A

A

V

F

K

K

I

L

A

A

R

R

A

H

-

Y

-

A

-

C

-

V

-

R

-

H

A

S

T

P

G

F

R

K

S

T

G

K

A

I

I

I

A

A

F

F

T

H

G

N

A

A

Y
x
F

H
|
H

G

G

R
|
R

T

S

H

L

Y

F

T

T

L

V

S

S

L

V

T

G

G

G

K

Q

V

P

N

K

P

-

Y

Y

S

S

A

D

G

G

M

F

G

G

L

P

M

K

P

P

G

A

H

D

V

I

Y

I

R

H

A

V

L

P

Y

F

P

-

C

N

A

D

L

L

H

H

G

A

V

V

S

-

D

-

E

K

A

A

I

V

A

M

S

D

I

D

H

H

R

-

I

-

F

-

K

-

N

-

D

-

A

-

P

-

E

-

D

-

I

T

A

C

A

A

I

V

E
|
E

P

P

V

I

Q

Q

G

G

E
|
E

G

G

F

V

Y

Q

A

A

S

T

P

P

A

E

F

F

M

L

Q

K

R

G

L

L

R

R

A

D

L

L

C

C

D

D

E

E

H

H

G

Q

I

A

M

L

L

L

I

V

A

F

D
|
D

E

E

V
|
V

Q
|
Q

S

C

G

G

A

M

G

G

R

R

T

T

G

G

T

D

L

L

F

F

A

A

M

Y

E

M

Q

H

M

Y

G

G

V

V

A

T

A

P

D

D

I

I

T

L

T

T

F

S

A

A

K
|
K

S

A

I

L

A

G

G

G

F

F

P

P

L

V

A

S

G

A

V

M

T

L

G

T

R

T

A

Q

E

E

V

I

M

A

D

S

A

A

I

-

A

-

P

-

G

-

L

F

G

G

G

S

T
|
T

Y

Y

A

G

G

G

N

N

P

P

I

L

A

A

C

C

A

A

A

V

A

A

L

G

A

A

V

T

L

F

Q

D

I

I

F

I

E

N

Q

T

E

P

N

E

L

V

L

L

E

Q

K

G

A

I

N

H

Q

T

L

K

G

R

D

Q

T

Q

L

F

R

V

Q

Q

G

H

L

L

L

Q

A

A

I

I

A

D

E

E

D

Q

H

F

P

D

E

I

I

F

G

S

D

D

V

I

R

R

G

G

L

M

G

G

A

L

M

L

I

I

A

G

I

A

E

E

L

L

F

-

E

-

G

-

D

-

R

-

S

-

K

K

P

P

N

K

A

Y

R

K

L

G

T

R

A

A

-

R

D

D

I

F

V

L

A

Y

R

A

A

G

R

A

D

E

K

A

G

G

L

V

I

M

L

V

L

L

S

N

C

A

G

G

P

A

Y

-

N

D

V

V

L

M

R
|
R

I

F

L

A

V

P

P

S

L

L

T

V

I

V

E

E

A

A

Q

D

I

I

E

H

Q

E

G

G

L

M

K

Q

I

R

I

F

A

A

active site: F136 (≠ Y138), E188 (= E206), D221 (= D239), Q224 (= Q242), K250 (= K268), T274 (= T297), R367 (= R398)
binding (2S)-2-azanyl-4-[(E)-[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]oxy-butanoic acid: G103 (= G111), T104 (≠ S112), F136 (≠ Y138), H137 (= H139), R139 (= R141), E188 (= E206), E193 (= E211), D221 (= D239), V223 (= V241), K250 (= K268)
binding picric acid: K25 (≠ R29), K27 (≠ E31), W32 (= W36)

2pb0A Structure of biosynthetic n-acetylornithine aminotransferase from salmonella typhimurium: studies on substrate specificity and inhibitor binding (see paper)
37% identity, 91% coverage: 29:418/427 of query aligns to 19:381/389 of 2pb0A

query
sites
2pb0A

R

K

A

G

E

K

N

G

C

S

R

R

V

V

W

W

D

D

V

Q

E

Q

G

G

R

K

E

E

Y

Y

L

I

D

D

F

F

A

A

G

G

I

I

A

A

V

V

L

T

N

A

T

L

G

G

H

H

L

C

H

H

P

P

Q

A

V

L

V

V

A

E

A

A

V

L

E

K

D

S

Q

Q

L

G

K

E

K

T

L

L

S

W

H

H

T

T

C

S

F

-

Q

N

V

V

L

F

A

T

Y

N

E

E

P

P

Y

A

L

L

A

R

L

L

C

G

E

R

K

K

M

L

N

-

Q

-

K

-

V

I

P

D

G

A

D

T

F

F

A

A

K

E

K

R

T

V

L

L

L

F

V

M

T

N

T
x
S

G
|
G

S
x
T

E

E

A

A

V

N

E

E

N

T

A

A

V

F

K

K

I

L

A

A

R

R

A

H

-

Y

-

A

-

C

-

V

-

R

-

H

A

S

T

P

G

F

R

K

S

T

G

K

A

I

I

I

A

A

F

F

T

H

G

N

A

A

Y
x
F

H
|
H

G

G

R

R

T

S

H

L

Y

F

T

T

L

V

S

S

L

V

T

G

G

G

K

Q

V

P

N

K

P

-

Y

Y

S

S

A

D

G

G

M

F

G

G

L

P

M

K

P

P

G

A

H

D

V

I

Y

I

R

H

A

V

L

P

Y

F

P

-

C

N

A

D

L

L

H

H

G

A

V

V

S

-

D

-

E

K

A

A

I

V

A

M

S

D

I

D

H

H

R

-

I

-

F

-

K

-

N

-

D

-

A

-

P

-

E

-

D

-

I

T

A

C

A

A

I

V

E
|
E

P

P

V

I

Q

Q

G

G

E

E

G

G

F

V

Y

Q

A

A

S

T

P

P

A

E

F

F

M

L

Q

K

R

G

L

L

R

R

A

D

L

L

C

C

D

D

E

E

H

H

G

Q

I

A

M

L

L

L

I

V

A

F

D
|
D

E

E

V
|
V

Q
|
Q

S

C

G

G

A

M

G

G

R

R

T

T

G

G

T

D

L

L

F

F

A

A

M

Y

E

M

Q

H

M

Y

G

G

V

V

A

T

A

P

D

D

I

I

T

L

T

T

F

S

A

A

K
|
K

S

A

I

L

A

G

G

G

F

F

P

P

L

V

A

S

G

A

V

M

T

L

G

T

R

T

A

Q

E

E

V

I

M

A

D

S

A

A

I

-

A

-

P

-

G

-

L

F

G

G

G

S

T
|
T

Y

Y

A

G

G

G

N

N

P

P

I

L

A

A

C

C

A

A

A

V

A

A

L

G

A

A

V

A

L

F

Q

D

I

I

F

I

E

N

Q

T

E

P

N

E

L

V

L

L

E

Q

K

G

A

I

N

H

Q

T

L

K

G

R

D

Q

T

Q

L

F

R

V

Q

Q

G

H

L

L

L

Q

A

A

I

I

A

D

E

E

D

Q

H

F

P

D

E

I

I

F

G

S

D

D

V

I

R

R

G

G

L

M

G

G

A

L

M

L

I

I

A

G

I

A

E

E

L

L

F

-

E

-

G

-

D

-

R

-

S

-

K

K

P

P

N

K

A

Y

R

K

L

G

T

R

A

A

-

R

D

D

I

F

V

L

A

Y

R

A

A

G

R

A

D

E

K

A

G

G

L

V

I

M

L

V

L

L

S

N

C

A

G

G

P

A

Y

-

N

D

V

V

L

M

R
|
R

I

F

L

A

V

P

P

S

L

L

T

V

I

V

E

E

A

A

Q

D

I

I

E

H

Q

E

G

G

L

M

K

Q

I

R

I

F

A

A

active site: F130 (≠ Y138), E182 (= E206), D215 (= D239), Q218 (= Q242), K244 (= K268), T268 (= T297), R361 (= R398)
binding pyridoxal-5'-phosphate: S96 (≠ T110), G97 (= G111), T98 (≠ S112), F130 (≠ Y138), H131 (= H139), E182 (= E206), D215 (= D239), V217 (= V241), Q218 (= Q242), K244 (= K268)

Query Sequence

>WP_008805274.1 NCBI__GCF_000025465.1:WP_008805274.1
MNSNKAMMARRSDAVPRGVGQIHPIFAERAENCRVWDVEGREYLDFAGGIAVLNTGHLHP
QVVAAVEDQLKKLSHTCFQVLAYEPYLALCEKMNQKVPGDFAKKTLLVTTGSEAVENAVK
IARAATGRSGAIAFTGAYHGRTHYTLSLTGKVNPYSAGMGLMPGHVYRALYPCALHGVSD
DEAIASIHRIFKNDAAPEDIAAIIIEPVQGEGGFYAASPAFMQRLRALCDEHGIMLIADE
VQSGAGRTGTLFAMEQMGVAADITTFAKSIAGGFPLAGVTGRAEVMDAIAPGGLGGTYAG
NPIACAAALAVLQIFEQENLLEKANQLGDTLRQGLLAIAEDHPEIGDVRGLGAMIAIELF
EEGDRSKPNARLTADIVARARDKGLILLSCGPYYNVLRILVPLTIEEAQIEQGLKIIADC
FTEAKQA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory