SitesBLAST

M1 (= M1) modified: Initiator methionine, Removed
C2 (= C2) mutation C->A,S: Loss of glutamine-dependent activity but no effect on ammonia-dependent asparagine synthetase activity.
H30 (= H30) mutation to A: 4,5-fold decrease in glutamine affinity.
D34 (= D34) mutation D->N,E: Little effect on the kinetic properties.
H81 (= H81) mutation to A: 5-fold decrease in glutamine affinity.
A105 (= A105) mutation to H: Little effect on the kinetic properties.
E349 (= E349) mutation E->A,Q: Loss of glutamine- and ammonia-dependent synthetase activity, but still exhibits glutaminase activity.; mutation to D: 5-fold increase in affinity for aspartate when assaying both the glutamine- and ammonia-dependent synthetase reactions, and 2-fold decrease in kcat for these reactions. Modifies the product glutamate/asparagine stoichiometry.

1ct9A Crystal structure of asparagine synthetase b from escherichia coli (see paper)
91% identity, 93% coverage: 2:517/554 of query aligns to 1:497/497 of 1ct9A

query
sites
1ct9A

C
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G

active site: A1 (≠ C2), L50 (= L51), N74 (= N75), G75 (= G76), T305 (= T322), R308 (= R325), E332 (= E349), M366 (= M383)
binding adenosine monophosphate: L232 (= L233), L233 (= L234), S234 (= S235), S239 (= S240), A255 (= A272), V256 (= V273), D263 (= D280), M316 (= M333), S330 (= S347), G331 (= G348), E332 (= E349)
binding glutamine: A1 (≠ C2), R49 (= R50), L50 (= L51), I52 (= I53), V53 (= V54), N74 (= N75), G75 (= G76), E76 (= E77), D98 (= D99)

P78753 Probable asparagine synthetase [glutamine-hydrolyzing]; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
51% identity, 100% coverage: 1:554/554 of query aligns to 1:557/557 of P78753

query
sites
P78753

M

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S391 (≠ F384) modified: Phosphoserine
S489 (= S474) modified: Phosphoserine

P08243 Asparagine synthetase [glutamine-hydrolyzing]; Cell cycle control protein TS11; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Homo sapiens (Human) (see 7 papers)
38% identity, 95% coverage: 30:554/554 of query aligns to 26:560/561 of P08243

query
sites
P08243

H

H

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-

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x
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N

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E

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-

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Y

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I

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R

Q

P

K

K

E

E

Q

A

F

F

S

S

D

D

G

G

-

I

-

T

-

S

V

V

G

K

Y

N

S

S

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K

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E

E

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Y

A

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A

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K

H

Q

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I

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D

D

D

Q

A

Q

M

L

M

E

A

T

N

A

A

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A

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Q

R

K

F

F

P

P

Y

F

N

N

T

T

P

P

T

K

S

T

K

K

E

E

G

G

Y

Y

L

Y

Y

Y

R

R

E

Q

I

V

F

F

E

E

E

R

L

H

F

Y

P

P

L

-

P

-

S

-

A

-

E

-

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-

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-

P

-

G

-

G

G

P

R

S

A

V

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A

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C

L

S

S

S

H

A

Y

K

W

A

M

I

P

E

K

W

W

D

I

E

N

A

A

F

-

K

-

T

-

M

-

N

T

D

D

P

P

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|
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G

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Y

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K

V210 (≠ F197) to E: in dbSNP:rs1049674
F362 (≠ L346) to V: in ASNSD; dramatic reduction in protein abundance; dbSNP:rs398122973
R550 (= R544) to C: in ASNSD; increases level of protein abundance; dbSNP:rs398122974

Sites not aligning to the query:

2 active site, For GATase activity; C→A: Loss of the glutamine-dependent asparagine synthetase activity, while the ammonia-dependent activity remained unaffected.
6 A → E: in ASNSD; dramatic reduction in protein abundance; dbSNP:rs398122975

6gq3A Human asparagine synthetase (asns) in complex with 6-diazo-5-oxo-l- norleucine (don) at 1.85 a resolution (see paper)
38% identity, 86% coverage: 30:507/554 of query aligns to 25:501/509 of 6gq3A

query
sites
6gq3A

H

H

R

R

G

G

P

P

D

D

-

A

-

F

-

R

W

F

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E

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I

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A

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E

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R

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x
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|
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N

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A

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E

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A

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-

W

L

L

A

C

V
x
Y

N
|
N

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E
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E

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I

Y

Y

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N

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H

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A

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-

D

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x
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D

C

G

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I

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D

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A

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A

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Q

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K

F

F

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P

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F

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N

T

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P

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K

S

T

K

K

E

E

G

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active site: L49 (= L51), N74 (= N75), G75 (= G76), T324 (= T322), R327 (= R325)
binding 5-oxo-l-norleucine: R48 (= R50), V51 (≠ I53), V52 (= V54), Y73 (≠ V74), N74 (= N75), G75 (= G76), E76 (= E77), V95 (≠ S98), D96 (= D99)

Sites not aligning to the query:

active site: 4
binding 5-oxo-l-norleucine: 1

1q19A Carbapenam synthetase (see paper)
27% identity, 41% coverage: 231:456/554 of query aligns to 241:448/500 of 1q19A

query
sites
1q19A

G

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P

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I

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E

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M

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I

A

K

Q

A

G

M

Q

G

-

I

V

K

S

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C

V

M

L

L

S
x
T

G
|
G

E
x
Y

G
|
G

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S

D
|
D

E
x
L

V

L

F

F

G

G

Y

I

L

L

Y

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F

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A

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A

Y

P

D

N

N

A

P

K

N

E

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K
x
R

L

T

Q

R

A

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L

T

H

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M
x
E

F

F

D

-

C

-

A

-

R

-

A

A

N

T

K

H

A

G

M

A

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S

A

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H

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A

I

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N

H

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Q

D

D

Y

K

K

M

I

C

F

G

D

N

N

G

E

K

V

M

-

E

-

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K

H

N

V

I

L

L

R

R

E

E

C

Y

F

A

E

D

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-

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L

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P

A

K

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D

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I

A

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W

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K

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|
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E
x
I

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x
G

F
x
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active site: L318 (≠ T322), E321 (≠ R325), Y344 (≠ E349), E379 (≠ M383), K442 (= K450)
binding diphosphomethylphosphonic acid adenosyl ester: P243 (≠ L233), L244 (= L234), S245 (= S235), D249 (= D239), S250 (= S240), S268 (≠ A272), I269 (≠ V273), T342 (≠ S347), G343 (= G348), D347 (= D352), K442 (= K450), I443 (≠ E451), G444 (≠ Q452), I445 (≠ F453)
binding (2s,5s)-5-carboxymethylproline: Y344 (≠ E349), G345 (= G350), L348 (≠ E353), R373 (≠ K377), E379 (≠ M383)

Sites not aligning to the query:

active site: 56

Q9XB61 Carbapenam-3-carboxylate synthase; Carbapenam-3-carboxylate ligase; EC 6.3.3.6 from Pectobacterium carotovorum subsp. carotovorum (Erwinia carotovora subsp. carotovora) (see 3 papers)
27% identity, 41% coverage: 231:456/554 of query aligns to 242:449/503 of Q9XB61

query
sites
Q9XB61

G

G

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x
P

L
|
L

S
|
S

G
|
G

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A

A

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E
x
Y

G
|
G

S
|
S

D
|
D

E

L

V

L

F

F

G

G

Y

I

L

L

Y

K

-

P

F

G

H

A

K

Q

A

Y

P

D

N

N

A

P

K

N

E

Q

L

L

H

L

E

A

E

E

T
x
Q

V

V

R

Y

K
x
R

L

T

Q

R

A

W

L

T

H

G

M
x
E

F

F

D

-

C

-

A

-

R

-

A

A

N

T

K

H

A

G

M

A

S

S

A

C

W

Y

G

G

V

I

E

D

A

I

R

R

V

H

P

P

F

F

L

W

D

S

K

H

K

S

F

L

L

I

D

S

V

L

A

C

M

H

R

A

I

L

N

H

P

P

Q

D

D

Y

K

K

M

I

C

F

G

D

N

N

G

E

K

V

M

-

E

-

K
|
K

H

N

V

I

L

L

R

R

E

E

C

Y

F

A

E

D

S

S

-

L

-

Q

Y

L

L

L

P

P

A

K

S

D

V

I

A

V

W

W

R

R

Q

K

K
|
K

E
x
I

Q
x
G

F
x
I

S

H

D

E

G

G

244:251 (vs. 233:240, 88% identical) binding ATP
I270 (≠ V273) binding ATP
GYGSD 344:348 (≠ GEGSD 348:352) binding ATP
Y345 (≠ E349) mutation to A: Loss of activity.; mutation to F: Reduces catalytic efficiency.
G346 (= G350) binding substrate
Q371 (≠ T374) binding substrate
R374 (≠ K377) binding substrate
E380 (≠ M383) mutation to A: Loss of activity.; mutation to D: Reduces catalytic efficiency.; mutation to Q: Reduces catalytic efficiency.
K421 (= K430) binding ATP
K443 (= K450) mutation K->A,M: Loss of activity.
IGI 444:446 (≠ EQF 451:453) binding ATP

1mb9A Beta-lactam synthetase complexed with atp (see paper)
25% identity, 73% coverage: 52:454/554 of query aligns to 49:435/485 of 1mb9A

query
sites
1mb9A

S

S

I

V

V

A

D

P

V

D

N

R

A

A

G

V

A

A

Q

R

P

S

L

L

Y

T

N

G

A

A

E

P

K

T

T

T

H

A

A

V

L

L

A
|
A

V

-

N

-

G
|
G

E

E

I

I

Y

Y

N

N

H

R

Q

D

A

E

L

L

R

L

A

S

E

V

Y

L

G

P

D

A

R

G

Y

P

Q

A

F

P

Q

E

T

-

G

-

S

G

D

D

C

A

E

E

V

L

I

V

L

L

A

R

L

L

Y

L

Q

E

E

R

K

Y

G

D

P

L

E

H

F

A

L

F

D

R

D

L

L

V

Q

N

G

G

M

R

F

F

A

A

F

T

A

V

L

V

Y

R

D

T

S

G

E

D

K

R

D

-

A

-

Y

V

L

L

I

L

G

A

R

T

D

D

H

H

I

A

G

G

I

S

I

V

P

P

L

L

Y

Y

M

T

G

C

H

V

D

-

E

A

H

P

G

G

N

E

F

V

Y

R

V

A

A

S

S

T

E

E

M

A

K

K

A

A

L

L

-

A

-

F

-

P

-

L

-

A

-

D

-

A

-

R

V

V

P

A

V

G

C

L

R

T

T

G

I

V

K

Y

E

Q

F

V

P

P

A

A

G

G

S

A

Y

V

L

M

W

D

S

I

K

D

D

L

G

G

E

S

I

G

R

T

Q

A

Y

V

Y

T

Q

H

R

R

D

T

W

W

F

-

D

-

Y

T

D

P

A

G

V

L

K

S

D

R

N

R

V

I

T

L

D

P

K

E

N

G

E

E

-

A

-

V

-

A

L

V

R

R

Q

A

A

A

L

L

E

E

E

K

S

A

V

V

K

A

S

Q

H

R

L

V

M

T

-

P

S

G

D

D

V

T

P

P

Y

L

G

-

V

V

L
x
V

L
|
L

S
|
S

G

G

G
|
G

L

I

D
|
D

S
|
S

S

S

V

G

I

V

S

A

A

A

I

C

T

A

K

H

K

R

F

A

A

A

A

G

R

-

V

-

E

-

D

-

Q

-

E

-

R

-

S

-

E

-

A

-

W

-

P

-

Q

E

L

L

H

D

S

T

F

V

A
x
S

V
x
M

G

G

L

T

E

D

G

T

S

S

P

N

D

E

L

F

K

R

A

E

A

A

Q

R

E

A

V

V

A

V

N

D

H

H

L

L

G

R

T

T

V

R

H

H

H

R

E

E

I

I

H

T

F

I

T

P

V

T

Q

T

E

E

G

L

L

L

D

A

A

Q

I

L

R

P

D

Y

V

A

I

V

Y

W

H

A

I

S

E

E

T

S

Y

V

D

D

V

P

T
x
D

T

I

I

I

R

E

A
x
Y

S

L

T

L

P

P

M
x
L

Y

T

L

A

M

L

S

Y

R

R

K

A

I

L

K

D

A

G

M

P

G

E

I

-

K

R

M

R

V

I

L

L

S

T

G
|
G

E
x
Y

G

G

S

A

D
|
D

E

I

V

P

F

L

G

G

Y

M

L

H

Y

R

F

E

H

D

K

R

A

L

P

P

N

A

A

L

K

D

E

T

L

V

H

L

E

A

E

H

T

D

V

M

R

A

K

T

L

F

Q

D

A

G

L

L

H

N

M
x
E

F

M

D

S

C

P

A

V

R

L

A

S

N

T

K

L

A

A

M

-

S

G

A

H

W

W

G

T

V

T

E

H

A

-

R

-

V

-

P

P

F

Y

L

W

D

D

K

R

K

E

F

V

L

L

D

D

V

L

A

L

M

V

R

S

I

L

N

E

P

A

Q

G

D

L

K

K

M

R

C

R

G

-

N

H

G

G

K

R

M

-

E

D

K
|
K

H

W

V

V

L

L

R

R

E

A

C

A

F

M

E

A

S

D

Y

A

L

L

P

P

A

A

S

E

V

T

A

V

W

N

R

R

Q

P

K
|
K

E

S

Q

S

F

F

S

S

active site: A70 (= A73), G71 (= G76), D310 (≠ T322), Y336 (≠ E349), E370 (≠ M383), K431 (= K450)
binding adenosine monophosphate: V235 (≠ L233), L236 (= L234), S242 (= S240), S260 (≠ A272), M261 (≠ V273), Y314 (≠ A326), L318 (≠ M330), G335 (= G348), Y336 (≠ E349)
binding adenosine-5'-triphosphate: V235 (≠ L233), L236 (= L234), S237 (= S235), G239 (= G237), D241 (= D239), S242 (= S240), S260 (≠ A272), M261 (≠ V273), L318 (≠ M330), G335 (= G348), D339 (= D352), K411 (= K430), K431 (= K450)
binding magnesium ion: D241 (= D239), D339 (= D352)
binding pyrophosphate 2-: S237 (= S235), G239 (= G237), D241 (= D239), S242 (= S240), D339 (= D352), K411 (= K430), K431 (= K450)

1mc1A Beta-lactam synthetase with product (dgpc), amp and ppi (see paper)
24% identity, 74% coverage: 52:460/554 of query aligns to 44:437/491 of 1mc1A

query
sites
1mc1A

S

S

I

V

V

A

D

P

V

D

N

R

A

A

G

V

A

A

Q

R

P

S

L

L

Y

T

N

G

A

A

E

P

K

T

T

T

H

A

A

V

L

L

A
|
A

V

-

N

-

G
|
G

E

E

I

I

Y

Y

N

N

H

R

Q

D

A

E

L

L

R

L

A

S

E

V

Y

L

G

P

D

A

R

G

Y

P

Q

A

F

P

Q

E

T

-

G

-

S

G

D

D

C

A

E

E

V

L

I

V

L

L

A

R

L

L

Y

L

Q

E

E

R

K

Y

G

D

P

L

E

H

F

A

L

F

D

R

D

L

L

V

Q

N

G

G

M

R

F

F

A

A

F

T

A

V

L

V

Y

R

D

T

S

G

E

D

K

R

D

-

A

-

Y

V

L

L

I

L

G

A

R

T

D

D

H

H

I

A

G

G

I

S

I

V

P

P

L

L

Y

Y

M

T

G

C

H

V

D

-

E

A

H

P

G

G

N

E

F

V

Y

R

V

A

A

S

S

T

E

E

M

A

K

K

A

A

L

L

-

A

-

G

-

F

-

P

-

L

-

A

-

D

-

A

-

R

V

V

P

A

V

G

C

L

R

T

T

G

I

V

K

Y

E

Q

F

V

P

P

A

A

G

G

S

A

Y

V

L

M

W

D

S

I

K

D

D

L

G

G

E

S

I

G

R

T

Q

A

Y

V

Y

T

Q

H

R

R

D

T

W

W

F

-

D

-

Y

T

D

P

A

G

V

L

K

S

D

R

N

R

V

I

T

L

D

P

K

E

N

G

E

E

-

A

-

V

-

A

L

V

R

R

Q

A

A

A

L

L

E

E

E

K

S

A

V

V

K

A

S

Q

H

R

L

V

M

T

-

P

S

G

D

D

V

T

P

P

Y

L

G

-

V

V

L
x
V

L

L

S
|
S

G

G

G
|
G

L

I

D
|
D

S
|
S

S

S

V

G

I

V

S

A

A

A

I

C

T

A

K

H

K

R

F

A

A

A

A

G

R

-

V

-

E

-

D

-

Q

-

E

-

R

-

S

-

E

-

A

-

W

-

P

-

Q

E

L

L

H

D

S

T

F

V

A
x
S

V
x
M

G

G

L

T

E

D

G

T

S

S

P

N

D

E

L

F

K

R

A

E

A

A

Q

R

E

A

V

V

A

V

N

D

H

H

L

L

G

R

T

T

V

R

H

H

H

R

E

E

I

I

H

T

F

I

T

P

V

T

Q

T

E

E

G

L

L

L

D

A

A

Q

I

L

R

P

D

Y

V

A

I

V

Y

W

H

A

I

S

E

E

T

S

Y

V

D

D

V

P

T
x
D

T

I

I

I

R

E

A
x
Y

S

L

T

L

P

P

M

L

Y

T

L

A

M

L

S

Y

R

R

K

A

I

L

K

D

A

G

M

P

G

E

I

-

K

R

M

R

V

I

L

L

S

T

G
|
G

E
x
Y

G
|
G

S

A

D
|
D

E
x
I

V

P

F

L

G

G

Y

M

L

H

Y

R

F

E

H

D

K

R

A

L

P

P

N

A

A

L

K

D

E

T

L

V

H

L

E

A

E

H

T
x
D

V

M

R

A

K

T

L

F

Q

D

A

G

L

L

H

N

M
x
E

F

M

D

S

C

P

A

V

R

L

A

S

N

T

K

L

A

A

M

-

S

G

A

H

W

W

G

T

V

T

E

H

A

-

R

-

V

-

P

P

F

Y

L

W

D

D

K

R

K

E

F

V

L

L

D

D

V

L

A

L

M

V

R

S

I

L

N

E

P

A

Q

G

D

L

K

K

M

R

C

R

G

-

N

H

G

G

K

R

M

-

E

D

K
|
K

H

W

V

V

L

L

R

R

E

A

C

A

F

M

E

A

S

D

Y

A

L

L

P

P

A

A

S

E

V

T

A

V

W

N

R

R

Q

P

K
|
K

E
x
L

Q

G

F
x
V

S

H

D

E

G

G

V

S

G

G

Y

T

S

T

active site: A65 (= A73), G66 (= G76), D306 (≠ T322), Y332 (≠ E349), E366 (≠ M383), K427 (= K450)
binding adenosine monophosphate: V231 (≠ L233), S233 (= S235), S238 (= S240), S256 (≠ A272), M257 (≠ V273), G331 (= G348), K427 (= K450), V430 (≠ F453)
binding magnesium ion: D237 (= D239), D335 (= D352)
binding deoxyguanidinoproclavaminic acid: Y310 (≠ A326), Y332 (≠ E349), G333 (= G350), I336 (≠ E353), D357 (≠ T374), E366 (≠ M383), K427 (= K450)
binding pyrophosphate 2-: S233 (= S235), G235 (= G237), D237 (= D239), S238 (= S240), D335 (= D352), K407 (= K430), K427 (= K450), L428 (≠ E451)

1mbzA Beta-lactam synthetase with trapped intermediate (see paper)
24% identity, 74% coverage: 52:460/554 of query aligns to 48:442/496 of 1mbzA

query
sites
1mbzA

S

S

I

V

V

A

D

P

V

D

N

R

A

A

G

V

A

A

Q

R

P

S

L

L

Y

T

N

G

A

A

E

P

K

T

T

T

H

A

A

V

L

L

A
|
A

V

-

N

-

G
|
G

E

E

I

I

Y

Y

N

N

H

R

Q

D

A

E

L

L

R

L

A

S

E

V

Y

L

G

P

D

A

R

G

Y

P

Q

A

F

P

Q

E

T

-

G

-

S

G

D

D

C

A

E

E

V

L

I

V

L

L

A

R

L

L

Y

L

Q

E

E

R

K

Y

G

D

P

L

E

H

F

A

L

F

D

R

D

L

L

V

Q

N

G

G

M

R

F

F

A

A

F

T

A

V

L

V

Y

R

D

T

S

G

E

D

K

R

D

-

A

-

Y

V

L

L

I

L

G

A

R

T

D

D

H

H

I

A

G

G

I

S

I

V

P

P

L

L

Y

Y

M

T

G

C

H

V

D

A

E

P

H

-

G

G

N

E

F

V

Y

R

V

A

A

S

S

T

E

E

M

A

K

K

A

A

L

L

-

A

-

H

-

G

-

F

-

P

-

L

-

A

-

D

-

A

-

R

V

V

P

A

V

G

C

L

R

T

T

G

I

V

K

Y

E

Q

F

V

P

P

A

A

G

G

S

A

Y

V

L

M

W

D

S

I

K

D

D

L

G

G

E

S

I

G

R

T

Q

A

Y

V

Y

T

Q

H

R

R

D

T

W

W

F

-

D

-

Y

T

D

P

A

G

V

L

K

S

D

R

N

R

V

I

T

L

D

P

K

E

N

G

E

E

-

A

-

V

-

A

L

V

R

R

Q

A

A

A

L

L

E

E

E

K

S

A

V

V

K

A

S

Q

H

R

L

V

M

T

-

P

S

G

D

D

V

T

P

P

Y

L

G

-

V

V

L
x
V

L
|
L

S
|
S

G

G

G
|
G

L

I

D
|
D

S
|
S

S

S

V

G

I

V

S

A

A

A

I

C

T

A

K

H

K

R

F

A

A

A

A

G

R

-

V

-

E

-

D

-

Q

-

E

-

R

-

S

-

E

-

A

-

W

-

P

-

Q

E

L

L

H

D

S

T

F

V

A
x
S

V
x
M

G

G

L

T

E

D

G

T

S

S

P

N

D

E

L

F

K

R

A

E

A

A

Q

R

E

A

V

V

A

V

N

D

H

H

L

L

G

R

T

T

V

R

H

H

H

R

E

E

I

I

H

T

F

I

T

P

V

T

Q

T

E

E

G

L

L

L

D

A

A

Q

I

L

R

P

D

Y

V

A

I

V

Y

W

H

A

I

S

E

E

T

S

Y

V

D

D

V

P

T
x
D

T

I

I

I

R

E

A
x
Y

S

L

T

L

P

P

M
x
L

Y

T

L

A

M

L

S

Y

R

R

K

A

I

L

K

D

A

G

M

P

G

E

I

-

K

R

M

R

V

I

L

L

S

T

G
|
G

E
x
Y

G
|
G

S

A

D
|
D

E
x
I

V

P

F

L

G

G

Y

M

L

H

Y

R

F

E

H

D

K

R

A

L

P

P

N

A

A

L

K

D

E

T

L

V

H

L

E

A

E

H

T
x
D

V

M

R

A

K

T

L

F

Q

D

A

G

L

L

H

N

M
x
E

F

M

D

S

C

P

A

V

R

L

A

S

N

T

K

L

A

A

M

-

S

G

A

H

W

W

G

T

V

T

E

H

A

-

R

-

V

-

P

P

F

Y

L

W

D

D

K

R

K

E

F

V

L

L

D

D

V

L

A

L

M

V

R

S

I

L

N

E

P

A

Q

G

D

L

K

K

M

R

C

R

G

-

N

H

G

G

K

R

M

-

E

D

K
|
K

H

W

V

V

L

L

R

R

E

A

C

A

F

M

E

A

S

D

Y

A

L

L

P

P

A

A

S

E

V

T

A

V

W

N

R

R

Q

P

K
|
K

E
x
L

Q
x
G

F
x
V

S

H

D

E

G

G

V

S

G

G

Y

T

S

T

active site: A69 (= A73), G70 (= G76), D311 (≠ T322), Y337 (≠ E349), E371 (≠ M383), K432 (= K450)
binding arginine-n-methylcarbonyl phosphoric acid 5'-adenosine ester: V236 (≠ L233), L237 (= L234), S238 (= S235), S243 (= S240), S261 (≠ A272), M262 (≠ V273), Y315 (≠ A326), L319 (≠ M330), G336 (= G348), Y337 (≠ E349), G338 (= G350), D340 (= D352), I341 (≠ E353), D362 (≠ T374), E371 (≠ M383), K432 (= K450), G434 (≠ Q452), V435 (≠ F453)
binding magnesium ion: D242 (= D239), D340 (= D352)
binding pyrophosphate 2-: S238 (= S235), G240 (= G237), D242 (= D239), S243 (= S240), D340 (= D352), K412 (= K430), K432 (= K450), L433 (≠ E451)

1jgtB Crystal structure of beta-lactam synthetase (see paper)
24% identity, 72% coverage: 52:450/554 of query aligns to 52:440/500 of 1jgtB

query
sites
1jgtB

S

S

I

V

V

A

D

P

V

D

N

R

A

A

G

V

A

A

Q

R

P

S

L

L

Y

T

N

G

A

A

E

P

K

T

T

T

H

A

A

V

L

L

A
|
A

V

-

N

-

G
|
G

E

E

I

I

Y

Y

N

N

H

R

Q

D

A

E

L

L

R

L

A

S

E

V

Y

L

G

P

D

A

R

G

Y

P

Q

A

F

P

Q

E

T

-

G

-

S

G

D

D

C

A

E

E

V

L

I

V

L

L

A

R

L

L

Y

L

Q

E

E

R

K

Y

G

D

P

L

E

H

F

A

L

F

D

R

D

L

L

V

Q

N

G

G

M

R

F

F

A

A

F

T

A

V

L

V

Y

R

D

T

S

G

E

D

K

R

D

-

A

-

Y

V

L

L

I

L

G

A

R

T

D

D

H

H

I

A

G

G

I

S

I

V

P

P

L

L

Y

Y

M

T

G

C

H

V

D

-

E

A

H

P

G

G

N

E

F

V

Y

R

V

A

A

S

S

T

E

E

M

A

K

K

A

A

L

L

-

A

-

H

-

R

-

D

-

P

-

K

-

G

-

F

-

P

-

L

-

A

-

D

-

A

-

R

V

V

P

A

V

G

C

L

R

T

T

G

I

V

K

Y

E

Q

F

V

P

P

A

A

G

G

S

A

Y

V

L

M

W

D

S

I

K

D

D

L

G

G

E

S

I

G

R

T

Q

A

Y

V

Y

T

Q

H

R

R

D

T

W

W

F

-

D

-

Y

T

D

P

A

G

V

L

K

S

D

R

N

R

V

I

T

L

D

P

K

E

N

G

E

E

-

A

-

V

-

A

L

V

R

R

Q

A

A

A

L

L

E

E

E

K

S

A

V

V

K

A

S

Q

H

R

L

V

M

T

-

P

S

G

D

D

V

T

P

P

Y

L

G

-

V

V

L
x
V

L
|
L

S
|
S

G

G

G
|
G

L
x
I

D
|
D

S
|
S

S

S

V

G

I

V

S

A

A

A

I

C

T

A

K

H

K

R

F

A

A

A

A

G

R

-

V

-

E

-

D

-

Q

-

E

-

R

-

S

-

E

-

A

-

W

-

P

-

Q

E

L

L

H

D

S

T

F

V

A
x
S

V
x
M

G

G

L

T

E

D

G

T

S

S

P

N

D

E

L

F

K

R

A

E

A

A

Q

R

E

A

V

V

A

V

N

D

H

H

L

L

G

R

T

T

V

R

H

H

H

R

E

E

I

I

H

T

F

I

T

P

V

T

Q

T

E

E

G

L

L

L

D

A

A

Q

I

L

R

P

D

Y

V

A

I

V

Y

W

H

A

I

S

E

E

T

S

Y

V

D

D

V

P

T
x
D

T

I

I

I

R

E

A
x
Y

S

L

T

L

P

P

M
x
L

Y

T

L

A

M

L

S

Y

R

R

K

A

I

L

K

D

A

G

M

P

G

E

I

-

K

R

M

R

V

I

L

L

S

T

G
|
G

E
x
Y

G
|
G

S

A

D
|
D

E
x
I

V

P

F

L

G

G

Y
x
M

L

H

Y

R

F

E

H

D

K

R

A

L

P

P

N

A

A

L

K

D

E

T

L

V

H

L

E

A

E

H

T
x
D

V

M

R

A

K

T

L

F

Q

D

A

G

L

L

H

N

M
x
E

F

M

D

S

C

P

A

V

R

L

A

S

N

T

K

L

A

A

M

-

S

G

A

H

W

W

G

T

V

T

E

H

A

-

R

-

V

-

P

P

F

Y

L

W

D

D

K

R

K

E

F

V

L

L

D

D

V

L

A

L

M

V

R

S

I

L

N

E

P

A

Q

G

D

L

K

K

M

R

C

R

G

-

N

H

G

G

K

R

M

-

E

D

K
|
K

H

W

V

V

L

L

R

R

E

A

C

A

F

M

E

A

S

D

Y

A

L

L

P

P

A

A

S

E

V

T

A

V

W

N

R

R

Q

P

K
|
K

active site: A73 (= A73), G74 (= G76), D319 (≠ T322), Y345 (≠ E349), E379 (≠ M383), K440 (= K450)
binding diphosphomethylphosphonic acid adenosyl ester: V244 (≠ L233), L245 (= L234), S246 (= S235), G248 (= G237), I249 (≠ L238), D250 (= D239), S251 (= S240), S269 (≠ A272), M270 (≠ V273), L327 (≠ M330), G344 (= G348), Y345 (≠ E349), D348 (= D352), K420 (= K430), K440 (= K450)
binding n2-(carboxyethyl)-l-arginine: Y323 (≠ A326), Y345 (≠ E349), G346 (= G350), D348 (= D352), I349 (≠ E353), M354 (≠ Y358), D370 (≠ T374), E379 (≠ M383)
binding magnesium ion: D250 (= D239), D348 (= D352)

Query Sequence

>WP_008805652.1 NCBI__GCF_000025465.1:WP_008805652.1
MCSIFGVLDIKTDAGELRKKALELSRLMRHRGPDWSGVYASDKAILAHERLSIVDVNAGA
QPLYNAEKTHALAVNGEIYNHQALRAEYGDRYQFQTGSDCEVILALYQEKGPEFLDDLQG
MFAFALYDSEKDAYLIGRDHIGIIPLYMGHDEHGNFYVASEMKALVPVCRTIKEFPAGSY
LWSKDGEIRQYYQRDWFDYDAVKDNVTDKNELRQALEESVKSHLMSDVPYGVLLSGGLDS
SVISAITKKFAARRVEDQERSEAWWPQLHSFAVGLEGSPDLKAAQEVANHLGTVHHEIHF
TVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMSRKIKAMGIKMVLSGEGSDEVFGGYLY
FHKAPNAKELHEETVRKLQALHMFDCARANKAMSAWGVEARVPFLDKKFLDVAMRINPQD
KMCGNGKMEKHVLRECFESYLPASVAWRQKEQFSDGVGYSWIDTLKEVAAKQISDQQLET
ASFRFPYNTPTSKEGYLYREIFEELFPLPSAAECVPGGPSVACSSAKAIEWDEAFKTMND
PSGRAVGVHQSAYK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory