SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_008807017.1 NCBI__GCF_000025465.1:WP_008807017.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6llaB Crystal structure of providencia alcalifaciens 3-dehydroquinate synthase (dhqs) in complex with mg2+ and NAD (see paper)
75% identity, 98% coverage: 1:357/364 of query aligns to 2:359/363 of 6llaB

query
sites
6llaB

M

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active site: R121 (= R120), K143 (= K142), E185 (= E184), K227 (= K226), E237 (= E236), R242 (= R240), N246 (= N244), H249 (= H247), H253 (= H251), H266 (= H264)
binding magnesium ion: E185 (= E184), H249 (= H247), H266 (= H264)
binding nicotinamide-adenine-dinucleotide: L46 (= L45), D72 (= D71), E74 (= E73), K77 (= K76), G105 (= G104), G106 (= G105), V107 (= V106), D110 (= D109), T130 (= T129), T131 (= T130), L133 (= L132), D137 (= D136), K143 (= K142), T173 (= T172), L174 (= L173), E178 (= E177)

6lk2A Crystal structure of providencia alcalifaciens 3-dehydroquinate synthase (dhqs) in complex with mg2+, NAD and chlorogenic acid (see paper)
75% identity, 98% coverage: 1:357/364 of query aligns to 2:355/357 of 6lk2A

query
sites
6lk2A

M

M

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E

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active site: R121 (= R120), K143 (= K142), E185 (= E184), K227 (= K226), R238 (= R240), N242 (= N244), H245 (= H247), H249 (= H251), H262 (= H264)
binding (1R,3R,4S,5R)-3-[3-[3,4-bis(oxidanyl)phenyl]propanoyloxy]-1,4,5-tris(oxidanyl)cyclohexane-1-carboxylic acid: D137 (= D136), E185 (= E184), K227 (= K226), R238 (= R240), N242 (= N244), H245 (= H247), T246 (= T248), H249 (= H251), H262 (= H264)
binding magnesium ion: E185 (= E184), H245 (= H247), H262 (= H264)
binding nicotinamide-adenine-dinucleotide: L46 (= L45), D72 (= D71), E74 (= E73), K77 (= K76), G105 (= G104), G106 (= G105), V107 (= V106), D110 (= D109), T130 (= T129), T131 (= T130), L133 (= L132), D137 (= D136), S138 (= S137), C170 (= C169), T173 (= T172), L174 (= L173), P175 (= P174), E178 (= E177)

Q9KNV2 3-dehydroquinate synthase; DHQS; EC 4.2.3.4 from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
63% identity, 98% coverage: 1:356/364 of query aligns to 1:355/361 of Q9KNV2

query
sites
Q9KNV2

M

M

E

E

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V

M

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V

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N41 (= N42) binding NAD(+)
DGEQYK 70:75 (= DGEQYK 71:76) binding NAD(+)
GVIGD 104:108 (= GVIGD 105:109) binding NAD(+)
TT 128:129 (= TT 129:130) binding NAD(+)
K141 (= K142) binding NAD(+)
KN 150:151 (= KN 151:152) binding NAD(+)
CLTT 168:171 (≠ CLKT 169:172) binding NAD(+)

3okfA 2.5 angstrom resolution crystal structure of 3-dehydroquinate synthase (arob) from vibrio cholerae
63% identity, 98% coverage: 1:356/364 of query aligns to 2:354/360 of 3okfA

query
sites
3okfA

M

M

E

E

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R

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T

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L

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G

E

E

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I

I

A

G

A

A

G

G

L

L

F

F

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D

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P

P

A

A

S

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L

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K

D

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Q

K

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M

V

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I

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V

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L

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active site: R120 (= R120), K142 (= K142), E184 (= E184), K226 (= K226), R238 (= R240), N242 (= N244), H245 (= H247), H249 (= H251), H262 (= H264)
binding nicotinamide-adenine-dinucleotide: N42 (= N42), L48 (= L48), D71 (= D71), E73 (= E73), K76 (= K76), G104 (= G104), G105 (= G105), V106 (= V106), D109 (= D109), T129 (= T129), T130 (= T130), L132 (= L132), D136 (= D136), T172 (= T172), L173 (= L173), E177 (= E177)

3zokA Structure of 3-dehydroquinate synthase from actinidia chinensis in complex with NAD (see paper)
56% identity, 90% coverage: 6:334/364 of query aligns to 6:336/365 of 3zokA

query
sites
3zokA

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active site: R122 (= R120), K144 (= K142), E186 (= E184), K228 (= K226), E238 (= E236), R242 (= R240), N246 (= N244), H249 (= H247), H253 (= H251), H266 (= H264)
binding glycine: K144 (= K142), K228 (= K226), R242 (= R240)
binding nicotinamide-adenine-dinucleotide: T44 (= T44), V45 (≠ L45), D73 (= D71), E75 (= E73), K78 (= K76), G106 (= G104), G107 (= G105), V108 (= V106), D111 (= D109), T131 (= T129), T132 (= T130), M134 (≠ L132), D138 (= D136), S139 (= S137), K144 (= K142), K153 (= K151), T174 (= T172), L175 (= L173), E179 (= E177), H266 (= H264)

U3KRF2 3-dehydroquinate synthase, chloroplastic; EC 4.2.3.4 from Actinidia chinensis var. chinensis (Chinese soft-hair kiwi) (see paper)
56% identity, 90% coverage: 6:334/364 of query aligns to 86:416/445 of U3KRF2

query
sites
U3KRF2

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N122 (= N42) binding NAD(+)
DGE 153:155 (= DGE 71:73) binding NAD(+)
K158 (= K76) binding NAD(+)
GGVIGD 186:191 (= GGVIGD 104:109) binding NAD(+)
TT 211:212 (= TT 129:130) binding NAD(+)
K224 (= K142) binding NAD(+)
K233 (= K151) binding NAD(+)
TLNT 251:254 (≠ CLKT 169:172) binding NAD(+)
K308 (= K226) binding NAD(+)

5eksA Structure of 3-dehydroquinate synthase from acinetobacter baumannii in complex with NAD
55% identity, 94% coverage: 1:341/364 of query aligns to 2:334/355 of 5eksA

query
sites
5eksA

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G

H
|
H

A

A

I

I

E

E

A

S

E

Y

M

L

G

G

Y

Y

G

G

N

T

W

W

L

L

H
|
H

G

G

E

E

A

A

V

V

A

A

A

T

G

G

M

M

V

V

M

M

A

A

A

A

H

D

T

L

S

S

E

Q

R

R

L

L

G

G

Q

W

F

I

R

S

A

N

Q

E

D

D

T

V

Q

A

R

R

I

T

I

K

D

K

L

I

L

I

K

Q

R

R

A

A

G

N

L

L

P

P

V

I

R

S

G

C

P

P

Q

Q

E

-

M

I

S

P

A

L

Q

D

A

D

Y

F

L

L

P

G

H

Y

M

M

M

A

R

H

D

D

K

K

K

K

V

V

L

-

A

-

G

-

D

Q

M

L

R

R

L

L

V

V

L

L

P

L

L

K

A

Q

I

L

G

G

S

Q

S

A

active site: R120 (= R120), K142 (= K142), E184 (= E184), K226 (= K226), R237 (= R240), N241 (= N244), H244 (= H247), H248 (= H251), H261 (= H264)
binding magnesium ion: E184 (= E184), H244 (= H247), H261 (= H264)
binding nicotinamide-adenine-dinucleotide: N42 (= N42), V45 (≠ L45), D71 (= D71), E73 (= E73), K76 (= K76), G104 (= G104), G105 (= G105), V106 (= V106), D109 (= D109), T129 (= T129), T130 (= T130), D136 (= D136), S137 (= S137), K142 (= K142), T172 (= T172), L173 (= L173), E177 (= E177)

Q5NFS1 3-dehydroquinate synthase; DHQS; EC 4.2.3.4 from Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4)
47% identity, 91% coverage: 12:344/364 of query aligns to 14:344/359 of Q5NFS1

query
sites
Q5NFS1

S

S

Y

Y

P

N

I

I

T

I

I

V

A

D

A

S

G

V

L

L

F

-

N

-

D

D

P

F

A

S

S

H

F

I

L

L

P

E

L

Y

K

V

S

T

G

N

D

K

Q

Q

A

V

M

L

L

V

V

V

T

T

N
|
N

E

T

T

T

L

V

A

A

P

K

L

L

Y

Y

L

L

D

T

T

K

V

F

R

L

S

A

V

A

L

L

E

V

Q

D

A

-

G

D

V

L

N

D

V

V

D

R

S

T

V

C

I

I

L

L

P

E

D
|
D

G
|
G

E
|
E

Q
|
Q

Y
|
Y

K
|
K

S

S

L

Q

A

Q

V

S

M

L

D

D

T

K

V

I

F

L

T

S

A

T

L

L

L

L

Q

E

K

N

P

H

H

F

G

T

R

R

D

N

T

S

T

T

-

V

L

L

V

V

A

A

L

L

G

G

G

G

G
|
G

V
|
V

I
|
I

G
|
G

D
|
D

L

I

T

T

G

G

F

F

A

A

A

A

A

A

S

I

Y

Y

Q

Q

R

R

G

G

V

I

R

D

F

F

I

I

Q

Q

V

I

P

P

T
|
T

T
|
T

L

L

L

L

S

S

Q

Q

V

V

D

D

S

S

S

S

V

V

G

G

G

G

K
|
K

T

T

A

A

V

I

N

N

H

H

P

Q

L

L

G

G

K

K

N

N

M

M

I

I

G

G

A

A

F

F

W

Y

Q

Q

P

P

V

K

S

V

V

V

V

Y

V

T

D

S

L

I

N

E

C
x
F

L
x
Y

K
|
K

T
|
T

L

L

P

P

A

Q

R

R

E

E

L

Y

A

I

S

A

G

G

L

M

A

A

E

E

V

V

I

V

K

K

Y

Y

G

A

V

F

I

I

L

-

D

S

G

K

E

D

F

F

F

Y

S

L

W

W

L

L

E

D

N

S

N

N

I

R

D

D

A

K

L

I

L

L

A

A

L

K

D

D

E

S

K

V

A

T

M

L

A

I

Y

E

C

M

I

V

R

K

R

R

C

S

C

C

E

Q

L

I

K

K

A

A

E

Q

V

V

V

V

A

A

A

M

D

D

E

E

R

K

E

E

-

L

T

T

G

G

L

A

R

R

A

A

L

I

L

L

N

N

L

F

G

G

H

H

T

T

F

F

G

G

H

H

A

A

I

I

E

E

A

K

E

C

M

Q

G

N

Y

Y

G

R

N

G

W

L

L

K

H

H

G

G

E

E

A

A

V

V

A

G

A

V

G

G

M

M

V

A

M

Q

A

A

A

I

H

D

T

F

S

S

E

H

R

Y

L

L

G

G

Q

L

F

I

R

S

A

Q

Q

Q

D

Q

T

A

Q

K

R

D

I

F

I

N

D

D

L

F

L

I

K

V

R

S

A

F

G

G

L

I

P

S

V

I

R

D

G

F

P

P

Q

N

E

D

M

I

S

C

A

Q

Q

K

A

E

Y

F

L

L

P

E

H

A

M

M

M

L

R

L

D

D

K

K

K

K

V

N

L

S

A

N

G

K

D

E

M

L

R

K

L

F

V

I

L

L

P

I

L

E

A

N

I

I

G

G

S

S

S

L

E

S

L

L

R

Q

N42 (= N42) binding NAD(+)
DGEQYK 70:75 (= DGEQYK 71:76) binding NAD(+)
GVIGD 105:109 (= GVIGD 105:109) binding NAD(+)
TT 129:130 (= TT 129:130) binding NAD(+)
K142 (= K142) binding NAD(+)
FYKT 169:172 (≠ CLKT 169:172) binding NAD(+)

5hvnA 3.0 angstrom crystal structure of 3-dehydroquinate synthase (arob) from francisella tularensis in complex with NAD.
46% identity, 91% coverage: 12:344/364 of query aligns to 17:339/354 of 5hvnA

query
sites
5hvnA

S

S

Y

Y

P

N

I

I

T

I

I

V

A

D

A

S

G

V

L

L

F

-

N

-

D

D

P

F

A

S

S

H

F

I

L

L

P

E

L

Y

K

V

S

T

G

N

D

K

Q

Q

A

V

M

L

L

V

V

V

T

T

N
|
N

E

T

T

T

L

V

A

A

P

K

L
|
L

Y

Y

L

L

D

T

T

K

V

F

R

L

S

A

V

A

L

L

E

V

Q

D

A

-

G

D

V

L

N

D

V

V

D

R

S

T

V

C

I

I

L

L

P

E

D
|
D

G

G

E
|
E

Q

Q

Y

Y

K
|
K

S

S

L

Q

A

Q

V

S

M

L

D

D

T

K

V

I

F

L

T

S

A

T

L

L

L

L

Q

E

K

N

P

H

H

F

G

T

R

R

D

N

T

S

T

T

-

V

L

L

V

V

A

A

L

L

G

G

G
|
G

G
|
G

V
|
V

I

I

G

G

D
|
D

L

I

T

T

G

G

F

F

A

A

A

A

A

A

S

I

Y

Y

Q

Q

R
|
R

G

G

V

I

R

D

F

F

I

I

Q

Q

V

I

P

P

T
|
T

T
|
T

L

L

L
|
L

S

S

Q

Q

V

V

D
|
D

S

S

S

S

V

V

G

G

G

G

K
|
K

T

T

A

A

V

I

N

N

H

H

P

Q

L

L

G

G

K

K

N

N

M

M

I

I

G

G

A

A

F

F

W

Y

Q

Q

P

P

V

K

S

V

V

V

V

Y

V

T

D

S

L

I

N

E

C
x
F

L

Y

K

K

T
|
T

L
|
L

P

P

A

Q

R

R

E
|
E

L

Y

A

I

S

A

G

G

L

M

A

A

E
|
E

V

V

I

V

K

K

Y

Y

G

A

V

F

I

I

L

-

D

S

G

K

E

D

F

F

F

Y

S

L

W

W

L

L

E

D

N

S

N

N

I

R

D

D

A

K

L

I

L

L

A

A

L

K

D

D

E

S

K

V

A

T

M

L

A

I

Y

E

C

M

I

V

R

K

R

R

C

S

C

C

E

Q

L

I

K
|
K

A

A

E

Q

V

V

V

V

A

A

A

M

D

D

E

-

R

-

E

-

T

T

G

G

L

A

R
|
R

A

A

L

I

L

L

N
|
N

L

F

G

G

H
|
H

T

T

F

F

G

G

H
|
H

A

A

I

I

E

E

A

K

E

C

M

Q

G

N

Y

Y

G

R

N

G

W

L

L

K

H
|
H

G

G

E

E

A

A

V

V

A

G

A

V

G

G

M

M

V

A

M

Q

A

A

A

I

H

D

T

F

S

S

E

H

R

Y

L

L

G

G

Q

L

F

I

R

S

A

Q

Q

Q

D

Q

T

A

Q

K

R

D

I

F

I

N

D

D

L

F

L

I

K

V

R

S

A

F

G

G

L

I

P

S

V

I

R

D

G

F

P

P

Q

N

E

D

M

I

S

C

A

Q

Q

K

A

E

Y

F

L

L

P

E

H

A

M

M

M

L

R

L

D

D

K

N

K

K

V

-

L

-

A

-

G

-

D

E

M

L

R

K

L

F

V

I

L

L

P

I

L

E

A

N

I

I

G

G

S

S

S

L

E

S

L

L

R

Q

active site: R123 (= R120), K145 (= K142), E187 (= E184), K228 (= K226), R239 (= R240), N243 (= N244), H246 (= H247), H250 (= H251), H263 (= H264)
binding nicotinamide-adenine-dinucleotide: N45 (= N42), L51 (= L48), D73 (= D71), E75 (= E73), K78 (= K76), G107 (= G104), G108 (= G105), V109 (= V106), D112 (= D109), T132 (= T129), T133 (= T130), L135 (= L132), D139 (= D136), K145 (= K142), F172 (≠ C169), T175 (= T172), L176 (= L173), E180 (= E177)

P56081 3-dehydroquinate synthase; DHQS; EC 4.2.3.4 from Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) (see paper)
36% identity, 98% coverage: 1:356/364 of query aligns to 1:339/343 of P56081

query
sites
P56081

M

M

E

Q

R

E

L

I

T

L

V

I

T

P

L

L

G

K

E

E

R

K

S

N

Y

Y

P

K

I

V

T

F

I

L

A

G

A

E

G

-

L

-

F

-

N

-

D

-

P

-

A

-

S

-

F

-

L

L

P

P

-

E

L

I

K

K

S

L

G

K

D

Q

Q

K

A

A

M

L

L

I

V

I

T

S

N

D

E

S

T

I

L

V

A

A

P

G

L

L

Y

H

L

L

D

P

T

Y

V

L

R

L

S

E

V

R

L

L

E

K

Q

A

A

L

G

E

V

V

N

R

V

V

D

-

S

-

V

C

I

V

L

I

P

E

D
x
S

G
|
G

E
|
E

Q
x
K

Y
|
Y

K
|
K

S

N

L

F

A

H

V

S

M

L

D

E

T

R

V

I

F

L

T

N

A

N

L

A

L

F

Q

E

K

M

P

Q

H

L

G

N

R

R

D

H

T

S

T

L

L

M

V

I

A

A

L

L

G

G

G

G

G
|
G

V
|
V

I

I

G

S

D

D

L

M

T

V

G

G

F

F

A

A

A

S

A

S

S

I

Y

Y

Q

F

R

R

G

G

V

I

R

D

F

F

I

I

Q

N

V

I

P

P

T
|
T

T
|
T

L

L

L

L

S

A

Q

Q

V

V

D

D

S

A

S

S

V

V

G

G

G

G

K

K

T

T

A

G

V

I

N

N

H

T

P

P

L

Y

G

G

K

K

N
|
N

M

L

I

I

G

G

A

S

F

F

W

H

Q

Q

P

P

V

K

S

A

V

V

V

Y

V

M

D

D

L

L

N

A

C
x
F

L
|
L

K
|
K

T
|
T

L

L

P

E

A

K

R

R

E

E

L

F

A

Q

S

A

G

G

L

V

A

A

E

E

V

I

I

I

K

K

Y

M

G

A

V

V

I

C

L

F

D

D

G

K

E

N

F

L

F

V

S

E

W

R

L

L

E

E

N

T

N

K

I

-

D

D

A

L

L

K

L

D

A

C

L

L

D

E

E

E

K

-

A

-

M

-

A

-

Y

-

C

V

I

I

R

F

R

Q

C

S

C

V

E

N

L

I

K

K

A

A

E

Q

V

V

V

V

A

V

A

Q

D

D

E

E

R

K

E

E

T

Q

G

N

L

I

R

R

A

A

L

G

L

L

N

N

L

Y

G

G

H

H

T

T

F

F

G

G

H

H

A

A

I

I

E

E

A

K

E

E

M

T

G

D

Y

Y

G

E

N

R

W

F

L

L

H

H

G

G

E

E

A

A

V

I

A

A

A

I

G

G

M

M

V

R

M

M

A

A

A

N

H

D

T

L

S

A

E

L

R

S

L

L

G

G

Q

M

F

L

R

T

A

L

Q

K

D

E

T

Y

Q

E

R

R

I

I

I

E

D

N

L

L

L

L

K

K

R

K

A

F

G

D

L

L

P

-

V

I

R

F

G

H

P

Y

Q

K

E

I

M

L

S

D

A

L

Q

Q

A

K

Y

F

L

Y

P

E

H

R

M

L

M

F

R

L

D

D

K

K

K

K

V

S

L

E

A

N

G

K

D

T

M

I

R

K

L

F

V

I

L

L

P

P

L

K

A

G

I

V

G

G

S

A

S

F

E

E

L

V

R

A

G

S

G

H

V

I

P

P

H

K

D

E

V

T

V

I

L

I

G

K

A

V

I

L

SGEKYK 61:66 (≠ DGEQYK 71:76) binding NAD(+)
GV 95:96 (= GV 105:106) binding NAD(+)
TT 119:120 (= TT 129:130) binding NAD(+)
N142 (= N152) binding NAD(+)
FLKT 159:162 (≠ CLKT 169:172) binding NAD(+)

6hqvA Pentafunctional arom complex from chaetomium thermophilum (see paper)
41% identity, 84% coverage: 38:341/364 of query aligns to 38:367/1555 of 6hqvA

query
sites
6hqvA

M

V

L

L

V

I

T

T

N
x
D

E

T

T
x
N

L
|
L

A

Y

P

T

L

T

Y

Y

L

V

D

P

T

P

V

F

R

Q

S

A

V

V

L

F

E

E

Q

A

A

A

G

A

-

P

-

R

-

D

V

V

N

R

V

L

D

L

S

T

V

Y

I

A

L

I

P

P

D

P

G

G

E
|
E

Q

Y

Y

S

K
|
K

S

S

L

R

A

E

V

T

M

K

D

A

T

E

V

I

F

E

T

D

A

W

L

M

L

L

Q

S

K

H

P

A

H

C

G

T

R

R

D

D

T

T

T

V

L

I

V

I

A

A

L

L

G

G

G
|
G

G
|
G

V
|
V

I

I

G

G

D
|
D

L

M

T

I

G

G

F

Y

A

V

A

A

A

A

S

T

Y

F

Q

M

R
|
R

G

G

V

V

R

R

F

F

I

V

Q

Q

V

V

P

P

T
|
T

T
|
T

L

L

L
|
L

S

A

Q

M

V

V

D
|
D

S
|
S

S

S

V

I

G

G

G

G

K
|
K

T

T

A

A

V

I

N

D

H

T

P

P

L

M

G

G

K
|
K

N

N

M

L

I

I

G

G

A

A

F

F

W

W

Q

Q

P

P

V

R

S

R

V

I

V

Y

V

I

D

D

L

L

N

A

C

F

L

L

K

E

T
|
T

L
|
L

P
|
P

A

V

R

R

E
|
E

L

F

A

I

S

N

G

G

L

M

A

A

E
|
E

V

V

I

I

K

K

Y

T

G

A

V

A

I

I

L

W

D

N

G

E

E

T

F

E

F

F

S

T

W

A

L

L

E

E

N

E

N

N

I

A

D

A

A

A

L

I

L

L

-

E

A

A

L

V

D

R

E

S

K

K

A

A

-

S

-

S

-

P

-

A

-

A

-

R

-

L

-

A

-

P

M

I

A

R

Y

H

C

I

I

L

R

K

R

R

-

I

-

V

-

L

-

G

C

S

C

A

E

R

L

V

K
|
K

A

A

E

E

V

V

V

V

A

S

A

A

D

D

E

E

R

R

E
|
E

T

G

G

G

L

L

R
|
R

A

N

L

L

L

L

N
|
N

L

F

G

G

H
|
H

T

S

F

I

G

G

H
|
H

A

A

I

Y

E

E

A

A

E

I

M

L

G

A

Y

-

G

P

N

Q

W

V

L

L

H
|
H

G

G

E

E

A

C

V

V

A

A

A

I

G

G

M

M

V

V

M

K

A

E

A

A

H

E

T

L

S

A

E

R

R

Y

L

L

G

G

Q

V

F

L

R

R

A

P

Q

S

D

A

T

V

Q

A

R

R

I

L

I

T

D

K

L

L

L

I

K

A

R

S

A

Y

G

D

L

L

P

P

-

T

-

S

-

V

-

H

-

D

-

K

-

R

-

I

-

A

-

K

V

L

R

S

G

A

P

G

Q

K

E

E

M

C

S

P

A

V

Q

D

A

V

Y

L

L

L

P

Q

H

K

M

M

M

A

R

V

D

D

K

K

K

K

V

N

L

E

A

G

G

R

D

K

M

K

R

K

L

I

V

V

L

L

P

L

L

S

A

A

I

I

G

G

S

K

S

T

active site: R123 (= R120), K145 (= K142), E187 (= E184), K243 (= K226), E253 (= E236), R257 (= R240), N261 (= N244), H264 (= H247), H268 (= H251), H280 (= H264)
binding glutamic acid: D139 (= D136), K145 (= K142), E187 (= E184), K243 (= K226), R257 (= R240), H264 (= H247), H280 (= H264)
binding nicotinamide-adenine-dinucleotide: D42 (≠ N42), N44 (≠ T44), L45 (= L45), E76 (= E73), K79 (= K76), G107 (= G104), G108 (= G105), V109 (= V106), D112 (= D109), T132 (= T129), T133 (= T130), L135 (= L132), D139 (= D136), S140 (= S137), K145 (= K142), K154 (= K151), T175 (= T172), L176 (= L173), P177 (= P174), E180 (= E177), H280 (= H264)
binding zinc ion: E187 (= E184), H264 (= H247), H280 (= H264)

Sites not aligning to the query:

binding (4S,5R)-4,5-dihydroxy-3-oxocyclohex-1-ene-1-carboxylic acid: 1060, 1062, 1181, 1224, 1232, 1242, 1243
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: 413, 562, 563, 874, 923, 924, 979, 1277, 1279, 1323, 1327, 1348, 1368, 1526

1dqsA Crystal structure of dehydroquinate synthase (dhqs) complexed with carbaphosphonate, NAD+ and zn2+ (see paper)
45% identity, 76% coverage: 70:347/364 of query aligns to 75:371/381 of 1dqsA

query
sites
1dqsA

P

P

D

P

G

G

E
|
E

Q

V

Y

S

K
|
K

S

S

L

R

A

Q

V

T

M

K

D

A

T

D

V

I

F

E

T

D

A

W

L

M

L

L

Q

S

K

Q

-

N

-

P

P

P

H

C

G

G

R

R

D

D

T

T

T

V

L

V

V

I

A

A

L

L

G

G

G
|
G

G
|
G

V
|
V

I

I

G

G

D
|
D

L

L

T

T

G

G

F

F

A

V

A

A

A

S

S

T

Y

Y

Q

M

R
|
R

G

G

V

V

R

R

F

Y

I

V

Q

Q

V

V

P

P

T
|
T

T
|
T

L

L

L
|
L

S

A

Q

M

V

V

D
|
D

S
|
S

S

S

V

I

G

G

G

G

K
|
K

T

T

A

A

V

I

N

D

H

T

P

P

L

L

G

G

K
|
K

N
|
N

M

L

I

I

G

G

A

A

F

I

W

W

Q

Q

P

P

V

T

S

K

V

I

V

Y

V

I

D

D

L

L

N

E

C

F

L

L

K

E

T
|
T

L

L

P
|
P

A

V

R

R

E
|
E

L

F

A

I

S

N

G

G

L

M

A

A

E
|
E

V

V

I

I

K

K

Y

T

G

A

V

A

I

I

L

S

D

S

G

E

E

E

F

E

F

F

S

T

W

A

L

L

E

E

N

E

N

N

I

A

D

E

A

T

L

I

L

L

A

K

L

A

-

V

-

R

-

R

-

R

-

F

-

E

-

G

D

T

E

E

K

E

A

I

M

L

A

K

Y

A

C

R

I

I

R

L

R

A

C

S

C

A

E

R

L

H

K
|
K

A

A

E

Y

V

V

V

V

A

S

A

A

D

D

E

E

R

R

E
|
E

T

G

G

G

L

L

R
|
R

A

N

L

L

L
|
L

N
|
N

L

W

G

G

H
|
H

T

S

F

I

G

G

H
|
H

A

A

I

I

E

E

A

A

E

I

M

L

G

-

Y

T

G

P

N

Q

W

I

L

L

H
|
H

G

G

E

E

A

C

V

V

A

A

A

I

G

G

M

M

V

V

M

K

A

E

A

A

H

E

T

L

S

A

E

R

R

H

L

L

G

G

Q

I

F

L

R

K

A

G

Q

V

D

A

T

V

Q

S

R

R

I

I

I

V

D

K

L

C

L

L

K

A

R

A

A

Y

G

G

L

L

P

P

-

T

-

S

-

L

-

K

-

D

-

A

-

R

-

I

-

R

-

K

V

L

R

T

G

A

P

G

Q

K

E

H

M

C

S

S

A

V

Q

D

A

Q

Y

L

L

M

P

F

H

N

M

M

M

A

R

L

D

D

K
|
K

K

K

V

N

L

D

A

G

G

P

D

K

M

K

R

K

L

I

V

V

L

L

P

L

L

S

A

A

I

I

G

G

S

T

S

P

-

Y

E

E

L

T

R

R

G

A

G

S

V

V

active site: R127 (= R120), K149 (= K142), E191 (= E184), K240 (= K226), E250 (= E236), R254 (= R240), N258 (= N244), H261 (= H247), H265 (= H251), H277 (= H264)
binding [1r-(1alpha,3beta,4alpha,5beta)]-5-(phosphonomethyl)-1,3,4-trihydroxycyclohexane-1-carboxylic acid: D143 (= D136), K149 (= K142), N159 (= N152), E191 (= E184), K240 (= K226), R254 (= R240), L257 (= L243), N258 (= N244), H261 (= H247), H265 (= H251), H277 (= H264), K346 (= K323)
binding nicotinamide-adenine-dinucleotide: E78 (= E73), K81 (= K76), G111 (= G104), G112 (= G105), V113 (= V106), D116 (= D109), T136 (= T129), T137 (= T130), L139 (= L132), D143 (= D136), S144 (= S137), K158 (= K151), T179 (= T172), P181 (= P174), E184 (= E177), H277 (= H264)
binding zinc ion: E191 (= E184), H261 (= H247), H277 (= H264)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 41, 43, 44

P07547 Pentafunctional AROM polypeptide; EC 4.2.3.4; EC 2.5.1.19; EC 2.7.1.71; EC 4.2.1.10; EC 1.1.1.25 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see 2 papers)
40% identity, 87% coverage: 33:347/364 of query aligns to 35:381/1583 of P07547

query
sites
P07547

S

S

G

S

D

T

Q

T

A

Y

M

V

L

L

V

V

T

T

N
x
D

E
x
T

T
x
N

L

I

A

G

P

S

L

I<