SitesBLAST

Q167 (≠ S158) mutation to N: Reduces D-fructose transport with no significant effect on D-glucose uptake. Reduces susceptibility to inhibition by compound 3361.

P0AGF4 D-xylose-proton symporter; D-xylose transporter from Escherichia coli (strain K12) (see paper)
23% identity, 82% coverage: 9:371/441 of query aligns to 3:411/491 of P0AGF4

query
sites
P0AGF4

T

T

Q

Q

A

Y

N

N

A

S

R

S

T

Y

V

I

F

F

R

S

V

I

T

T

S

L

-

V

-

A

-

T

-

L

G

G

N

G

F

L

L

L

E
x
F

M

G

Y

Y

D

D

F

T

M

A

V

V

F

I

G

S

Y

G

Y

T

A

V

S

E

A

S

I

L

A

N

D

T

T

V

F

F

F

V

P

A

T

P

Q

Q

S

N

P

L

F

S

A

E

S

S

L

A

M

A

L

N

T

S

L

L

M

L

T

G

F

F

G

C

A

V

G

A

F

S

L

A

M

L

R

-

P

-

L

I

G

G

A

C

I

I

V

I

-

G

-

A

L

L

G

G

A

G

Y

Y

I

C

D

S

H

N

H

R

-

F

G
|
G

R

R

R

D

G

S

L

L

L

K

I

I

T

A

L

A

G

V

L

L

M

F

-

F

-

I

-

S

-

G

-

V

-

G

-

S

-

A

-

W

-

P

A

E

L

L

G

G

T

F

L

T

T

S

I

I

A

N

-

P

-

D

-

N

C

T

T

V

P

P

S

V

Y

Y

H

L

S

A

I

-

G

G

M

Y

A

V

A

P

P

E

L

F

L

V

I

I

L

Y

A

-

G

-

R
|
R

L

I

Q

G

G

G

F

I

S

G

A

V

G

G

V

L

E

A

L

S

G

M

G

L

V

S

S

P

V

M

Y

Y

L

I

S

A

E
|
E

I

L

A

A

P

P

K

A

N

H

R

I

K
x
R

G

G

F

K

Y

L

V

V

S

S

W

F

Q

N

S

-

G

-

S

-

Q

-

Q
|
Q

I

F

A

A

V

I

I

I

F

F

A

G

A

Q

L

L

G

V

V

Y

T

C

L

V

N

N

Q

Y

Y

F

L

I

G

A

K

R

S

S

V

G

M

D

T

A

E

S

W

W

-

L

-

N

-

T

-

D

G

G

W

W

R

R

I

Y

P

M

F

F

I

A

V

S

G

E

C

C

L

I

-

P

I

A

V

L

P

L

F

F

L

L

F

M

Y

L

I

L

R

Y

R

T

M

V

L

P

E

E

E

S

T

P

E

R

A

W

F

L

N

M

Q

S

R

R

K

G

H

K

R

Q

P

E

S

Q

M

A

S

E

E

G

I

I

T

L

R

R

S

K

V

I

A

M

S

G

N

N

-

T

-

L

-

A

-

T

-

Q

-

A

-

V

-

Q

-

E

-

I

-

K

-

H

-

S

-

L

-

D

-

H

-

G

-

R

-

K

-

T

-

G

W

G

Q

R

L

L

V

L

L

M

V

F

G

G

M

V

F

G

M

V

V

I

V

V

T

I

T

G

T

V

-

M

-

L

-

S

-

I

-

F

-
x
Q

-

F

V

V

S

G

F

I

Y
x
N

L

V

I

V

T

L

A
x
Y

F

Y

T

A

P

P

T

E

Y

V

G

F

K

K

T

T

V

-

L

L

Q

G

F

A

T

S

A

T

Q

D

Q

I

S

A

F

L

L

L

V

Q

T

T

L

I

V

V

G

G

L

V

S

I

N
|
N

L

L

F

T

W

F

L

T

P

V

V

L

M

A

G

I

A

M

L

T

S

V

D

D

K

K

V

F

G
|
G

R
|
R

R

K

P

P

L

L

L

Q

L

I

F

G

S

A

A

L

L

G

M

M

L

A

L

I

T

G

S

M

Y

F

P

S

-

L

S

G

L

T

S

A

W

F

L

Y

V

T

A

Q

H

A

P

P

S

G

F

I

A

V

H

A

L

L

V

L

E

S

V

M

E

L

L

F

W

Y

L

V

S

A

F

A

M

F

Y

A

A

M

S

S

Y

W

N

G

G

P

A

V

M

C

V
x
W

V

V

C

L

L

L

T

S

E
|
E

I

I

M

F

P

P

A

N

E

A

V

I

R
|
R

A

G

S

K

G

A

F

L

S

A

M

I

A

A

F24 (≠ E26) mutation to A: Decreases xylose transport.
G83 (= G83) mutation to A: Abolishes xylose transport.
R133 (= R123) mutation R->C,H,L: Abolishes xylose transport.
E153 (= E143) mutation to A: Abolishes xylose transport.
R160 (≠ K150) mutation to A: Abolishes xylose transport.
Q168 (= Q162) binding beta-D-xylose; mutation to A: Abolishes xylose transport.
Q288 (vs. gap) mutation to A: Abolishes xylose transport.
QQ 288:289 (vs. gap) binding beta-D-xylose
Q289 (vs. gap) mutation to A: Strongly decreases xylose transport.
N294 (≠ Y254) binding beta-D-xylose; mutation to A: Abolishes xylose transport.
Y298 (≠ A258) mutation to A: Abolishes xylose transport.
N325 (= N286) mutation to A: No effect on xylose transport.
G340 (= G301) mutation to A: Abolishes xylose transport.
R341 (= R302) mutation R->A,W: Abolishes xylose transport.
W392 (≠ V352) binding beta-D-xylose; mutation to A: Abolishes xylose transport.
E397 (= E357) mutation to A: Abolishes xylose transport.
R404 (= R364) mutation to A: Strongly decreases xylose transport.

Sites not aligning to the query:

415 binding beta-D-xylose
416 W→A: Strongly decreases xylose transport.

4gc0A The structure of the mfs (major facilitator superfamily) proton:xylose symporter xyle bound to 6-bromo-6-deoxy-d-glucose (see paper)
22% identity, 56% coverage: 123:371/441 of query aligns to 129:407/475 of 4gc0A

query
sites
4gc0A

R

R

L

I

Q

G

G

G

F

I

S

G

A

V

G

G

V

L

E

A

L

S

G

M

G

L

V

S

S

P

V

M

Y

Y

L

I

S

A

E

E

I

L

A

A

P

P

K

A

N

H

R

I

K

R

G

G

F

K

Y

L

V

V

S

S

W

F

Q

N

S

-

G

-

S

-

Q

-

Q
|
Q

I

F

A

A

V

I

I

I

F

F

A

G

A

Q

L

L

G

V

V

Y

T

C

L

V

N

N

Q

Y

Y

F

L

I

G

A

K

R

S

S

V

G

M

D

T

A

E

S

W

W

-

L

-

N

-

T

-

D

G

G

W

W

R

R

I

Y

P

M

F

F

I

A

V

S

G

E

C

C

L

I

-

P

I

A

V

L

P

L

F

F

L

L

F

M

Y

L

I

L

R

Y

R

T

M

V

L

P

E

E

E

S

T

P

E

R

A

W

F

L

N

M

Q

S

R

R

K

G

H

K

R

Q

P

E

S

Q

M

A

S

E

E

G

I

I

T

L

R

R

S

K

V

I

A

M

S

G

N

N

-

T

-

L

-

A

-

T

-

Q

-

A

-

V

-

Q

-

E

-

I

-

K

-

H

-

S

-

L

-

D

-

H

-

G

-

R

-

K

-

T

-

G

W

G

Q

R

L

L

V

L

L

M

V

F

G

G

M

V

F

G

M

V

V

I

V

V

T

I

T

G

T

V

-

M

-

L

-

S

-

I

-

F

-
x
Q

-

Q

-

F

V

V

S

G

F

I

Y
x
N

L

V

I

V

T
x
L

A

Y

F

Y

T

A

P

P

T

E

Y

V

G

F

K

K

T

T

V

-

L

L

Q

G

F

A

T

S

A

T

Q

D

Q

I

S

A

F

L

L

L

V

Q

T

T

L

I

V

V

G

G

L

V

S

I

N

N

L

L

F

T

W

F

L

T

P

V

V

L

M

A

G

I

A

M

L

T

S

V

D

D

K

K

V

F

G

G

R

R

R

K

P

P

L

L

L

Q

L

I

F

G

S

A

A

L

L

G

M

M

L

A

L

I

T

G

S

M

Y

F

P

S

-

L

S

G

L

T

S

A

W

F

L

Y

V

T

A

Q

H

A

P

P

S

G

F

I

A

V

H

A

L

L

V

L

E

S

V

M

E

L

L

F

W

Y

L

V

S

A

F

A

M

F

Y

A

A

M

S

S

Y

W

N

G

G

P

A

V

M

C

V
x
W

V

V

C

L

L

L

T

S

E

E

I

I

M

F

P

P

A

N

E

A

V

I

R

R

A

G

S

K

G

A

F

L

S

A

M

I

A

A

binding 6-bromo-6-deoxy-beta-D-glucopyranose: Q164 (= Q162), Q284 (vs. gap), N290 (≠ Y254), L293 (≠ T257), W388 (≠ V352)

4gbzA The structure of the mfs (major facilitator superfamily) proton:xylose symporter xyle bound to d-glucose (see paper)
22% identity, 56% coverage: 123:371/441 of query aligns to 129:407/475 of 4gbzA

query
sites
4gbzA

R

R

L

I

Q

G

G

G

F

I

S

G

A

V

G

G

V

L

E

A

L

S

G

M

G

L

V

S

S

P

V

M

Y

Y

L

I

S

A

E

E

I

L

A

A

P

P

K

A

N

H

R

I

K

R

G

G

F

K

Y

L

V

V

S

S

W

F

Q

N

S

-

G

-

S

-

Q

-

Q
|
Q

I

F

A

A

V

I

I

I

F

F

A

G

A

Q

L

L

G

V

V

Y

T

C

L

V

N

N

Q

Y

Y

F

L

I

G

A

K

R

S

S

V

G

M

D

T

A

E

S

W

W

-

L

-

N

-

T

-

D

G

G

W

W

R

R

I

Y

P

M

F

F

I

A

V

S

G

E

C

C

L

I

-

P

I

A

V

L

P

L

F

F

L

L

F

M

Y

L

I

L

R

Y

R

T

M

V

L

P

E

E

E

S

T

P

E

R

A

W

F

L

N

M

Q

S

R

R

K

G

H

K

R

Q

P

E

S

Q

M

A

S

E

E

G

I

I

T

L

R

R

S

K

V

I

A

M

S

G

N

N

-

T

-

L

-

A

-

T

-

Q

-

A

-

V

-

Q

-

E

-

I

-

K

-

H

-

S

-

L

-

D

-

H

-

G

-

R

-

K

-

T

-

G

W

G

Q

R

L

L

V

L

L

M

V

F

G

G

M

V

F

G

M

V

V

I

V

V

T

I

T

G

T

V

-

M

-

L

-

S

-

I

-

F

-
x
Q

-

Q

-

F

V

V

S

G

F

I

Y
x
N

L

V

I

V

T

L

A

Y

F

Y

T

A

P

P

T

E

Y

V

G

F

K

K

T

T

V

-

L

L

Q

G

F

A

T

S

A

T

Q

D

Q

I

S

A

F

L

L

L

V

Q

T

T

L

I

V

V

G

G

L

V

S

I

N

N

L

L

F

T

W

F

L

T

P

V

V

L

M

A

G

I

A

M

L

T

S

V

D

D

K

K

V

F

G

G

R

R

R

K

P

P

L

L

L

Q

L

I

F

G

S

A

A

L

L

G

M

M

L

A

L

I

T

G

S

M

Y

F

P

S

-

L

S

G

L

T

S

A

W

F

L

Y

V

T

A

Q

H

A

P

P

S

G

F

I

A

V

H

A

L

L

V

L

E

S

V

M

E

L

L

F

W

Y

L

V

S

A

F

A

M

F

Y

A

A

M

S

S

Y

W

N

G

G

P

A

V

M

C

V
x
W

V

V

C

L

L

L

T

S

E

E

I

I

M

F

P

P

A

N

E

A

V

I

R

R

A

G

S

K

G

A

F

L

S

A

M

I

A

A

binding beta-D-glucopyranose: Q164 (= Q162), Q284 (vs. gap), N290 (≠ Y254), W388 (≠ V352)

4gbyA The structure of the mfs (major facilitator superfamily) proton:xylose symporter xyle bound to d-xylose (see paper)
22% identity, 56% coverage: 123:371/441 of query aligns to 129:407/475 of 4gbyA

query
sites
4gbyA

R

R

L

I

Q

G

G

G

F

I

S

G

A

V

G

G

V

L

E

A

L

S

G

M

G

L

V

S

S

P

V

M

Y

Y

L

I

S

A

E

E

I

L

A

A

P

P

K

A

N

H

R

I

K

R

G

G

F

K

Y

L

V

V

S

S

W

F

Q

N

S

-

G

-

S

-

Q

-

Q
|
Q

I

F

A

A

V

I

I

I

F

F

A

G

A

Q

L

L

G

V

V

Y

T

C

L

V

N

N

Q

Y

Y

F

L

I

G

A

K

R

S

S

V

G

M

D

T

A

E

S

W

W

-

L

-

N

-

T

-

D

G

G

W

W

R

R

I

Y

P

M

F

F

I

A

V

S

G

E

C

C

L

I

-

P

I

A

V

L

P

L

F

F

L

L

F

M

Y

L

I

L

R

Y

R

T

M

V

L

P

E

E

E

S

T

P

E

R

A

W

F

L

N

M

Q

S

R

R

K

G

H

K

R

Q

P

E

S

Q

M

A

S

E

E

G

I

I

T

L

R

R

S

K

V

I

A

M

S

G

N

N

-

T

-

L

-

A

-

T

-

Q

-

A

-

V

-

Q

-

E

-

I

-

K

-

H

-

S

-

L

-

D

-

H

-

G

-

R

-

K

-

T

-

G

W

G

Q

R

L

L

V

L

L

M

V

F

G

G

M

V

F

G

M

V

V

I

V

V

T

I

T

G

T

V

-

M

-

L

-

S

-

I

-

F

-
x
Q

-

Q

-

F

V

V

S

G

F

I

Y
x
N

L

V

I

V

T

L

A

Y

F

Y

T

A

P

P

T

E

Y

V

G

F

K

K

T

T

V

-

L

L

Q

G

F

A

T

S

A

T

Q

D

Q

I

S

A

F

L

L

L

V

Q

T

T

L

I

V

V

G

G

L

V

S

I

N

N

L

L

F

T

W

F

L

T

P

V

V

L

M

A

G

I

A

M

L

T

S

V

D

D

K

K

V

F

G

G

R

R

R

K

P

P

L

L

L

Q

L

I

F

G

S

A

A

L

L

G

M

M

L

A

L

I

T

G

S

M

Y

F

P

S

-

L

S

G

L

T

S

A

W

F

L

Y

V

T

A

Q

H

A

P

P

S

G

F

I

A

V

H

A

L

L

V

L

E

S

V

M

E

L

L

F

W

Y

L

V

S

A

F

A

M

F

Y

A

A

M

S

S

Y

W

N

G

G

P

A

V

M

C

V
x
W

V

V

C

L

L

L

T

S

E

E

I

I

M

F

P

P

A

N

E

A

V

I

R

R

A

G

S

K

G

A

F

L

S

A

M

I

A

A

binding beta-D-xylopyranose: Q164 (= Q162), Q284 (vs. gap), N290 (≠ Y254), W388 (≠ V352)

A0A0H2VG78 Glucose transporter GlcP; Glucose/H(+) symporter from Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200) (see paper)
24% identity, 78% coverage: 46:390/441 of query aligns to 38:398/446 of A0A0H2VG78

query
sites
A0A0H2VG78

P

P

T

L

Q

N

S

S

P

T

F

T

A

E

S

G

L

I

M

V

L

V

T

S

L

S

M

M

T

L

F

I

G

G

A

A

G

-

F

-

L

-

M

-

R

-

P

I

L

V

G

G

A

A

I

G

V

S

L

S

G

G

A

P

Y

L

I

A

D

D

H

K

H

L

G

G

R

R

G

L

L

V

L

M

I

L

T

I

L

A

G

I

L

V

M

F

A

I

L

I

G

G

T

A

L

L

T

I

I

L

A

A

C

A

T

S

P

T

S

N

Y

L

H

A

S

-

I

-

G

-

M

-

A

-

P

-

L

-

L

L

I

L

L

I

A

I

G

G

R
|
R

L

L

L

I

Q
x
I

G

G

F

L

S

A

A

V

G

G

V

G

E

S

L

M

G

S

G

T

V

V

S

P

V

V

Y

Y

L

L

S

S

E
|
E

I

M

A

A

P

P

K

T

N

E

R

Y

K

R

G

G

F

-

Y

-

V

-

S

-

W

-

Q

S

S

L

G

G

S

S

Q

L

Q

N

I
x
Q

A

L

V

M

I

I

F

T

A

I

A

G

L

I

L

L

G

A

V

A

T

Y

L

L

N

V

Q

N

Y

Y

L

A

G

F

K

A

S

D

V

I

M

E

T

-

E

-

W

-

G

G

W

W

R

R

I

-

P

-

F

W

I

M

V

L

G

G

C

L

L

A

I

V

V

V

P

P

F

S

L

V

F

I

Y

L

I

L

-

V

-

G

-

I

-

Y

-

F

-

M

-

P

-

E

-

S

-

P

R

R

R

W

M

L

L

L

E

E

-

N

-

R

E

N

T

E

E

E

A

A

F

A

N

R

Q

Q

-

V

-

M

-

K

-

I

-

T

-

Y

-

D

-

S

-

E

-

I

R

D

K

K

H

E

R

L

P

K

S

E

M

M

S

K

E

E

I

I

T

N

R

A

S

I

V

S

A

E

S

S

N

T

W

W

Q

T

L

V

V

I

-

K

-

S

-

P

L

W

V

L

G

G

M

R

F

I

M

L

V

I

V

V

T

G

T

C

T

I

V

F

S

A

F

I

Y

F

-
x
Q

-

F

-

I

-

G

-

I

-
x
N

-

A

L

V

I

I

T

F

A

Y

F

S

T

S

P

S

T

I

Y

F

G

A

K

K

T

A

V

G

L

L

Q

G

F

E

T

A

A

A

Q

-

S

S

F

I

L

L

V

G

T

S

L

V

L

G

V

I

G

G

L

T

S

I

N

N

L

V

F

L

W

V

L

T

P

I

V

V

M

A

G

I

A

F

L

V

S

V

D

D

K

K

V

I

G

D

R

R

R

K

P

K

L

L

L

V

-

G

-

N

-

I

-

G

L

M

F

I

S

A

A

S

L

L

M

L

L

I

L

M

T

A

S

-

Y

-

P

-

S

I

L

L

S

I

W

W

L

T

V

I

A

G

H

I

P

A

S

S

F

S

A

A

H

W

L

I

V

I

E

I

V

V

-

C

-

L

E

S

L

L

W

F

L

I

S

V

F

F

M

F

Y

G

A

I

S

S

Y

W

N

G

G

P

A

V

M

L

V
x
W

V

V

C

M

L

L

T

P

E

E

I

L

M

F

P

P

A

M

E

R

V

A

R

R

-

G

-

A

A

A

S

T

G

G

F

I

S

S

-

A

M

L

A

V

Y

L

S

N

L

I

A

G

T

T

A

L

I

I

F

V

G

S

G

L

F

F

T

F

P

P

A

I

I

L

S

S

S

D

Y

A

L

L

R102 (= R123) mutation to A: Loss of transport activity.
I105 (≠ Q126) mutation to S: Affects symport activity. May function as an uniporter.
E122 (= E143) mutation to A: Loss of transport activity.
Q137 (≠ I163) mutation to A: Loss of transport activity.
Q250 (vs. gap) mutation to A: Loss of transport activity.
Q251 (vs. gap) mutation to A: Loss of transport activity.
N256 (vs. gap) mutation to A: Loss of transport activity.
W357 (≠ V352) mutation to A: Loss of transport activity.

Sites not aligning to the query:

22 D→N: Affects symport activity. May function as an uniporter.

P43427 Solute carrier family 2, facilitated glucose transporter member 5; Fructose transporter; Glucose transporter type 5, small intestine; GLUT-5 from Rattus norvegicus (Rat) (see paper)
29% identity, 34% coverage: 53:201/441 of query aligns to 71:201/502 of P43427

query
sites
P43427

S

S

L

L

M

T

L

V

T

S

L

M

M

F

T

P

F

F

G

G

A

-

G

G

F

F

L

I

M

-

R

-

P

-

L

-

G

G

A

S

I

L

V

M

L

V

G

G

A

F

Y

L

I

V

D

N

H

N

H

L

G

G

R

R

R

K

R

G

G

A

L

L

I

F

T

N

L

N

G

I

L

F

M

S

A

I

L

L

G

P

T

A

L

I

T

L

I

M

A

G

C

C

T

S

P

K

S

I

Y

A

H

K

S

S

I

F

G

E

M

-

A

-

P

-

L

-

L

I

I

I

L

I

A

A

G

S

R

R

L

L

Q

V

G

G

F

I

S

C

A

A

G

G

V

I

E

S

L

S

G

N

G

V

V

V

S

P

V

M

Y

Y

L

L

S

G

E

E

I

L

A

A

P

P

K

K

N

N

R

L

K

R

G

G

F

A

Y

L

V

G

S

V

W

V

Q

P

S
x
Q

G

L

S

F

Q

I

Q

T

I

V

A

G

V

I

I

L

F

V

A

A

A

Q

L

L

L

F

G

G

V

L

T

-

L

-

N

-

Q

-

Y

-

L

-

G

-

K

R

S

S

V

V

M

L

-

A

T

S

E

E

W

E

G

G

W

W

R

-

I

-

P

P

F

I

I

L

V

L

G

G

C

L

L

T

I

G

V

V

P

P

Q166 (≠ S158) binding D-fructose; mutation to E: Impairs D-fructose binding.

Sites not aligning to the query:

31 binding D-fructose
287 binding D-fructose
295:297 binding D-fructose
386 binding D-fructose
418:419 binding D-fructose

Q3T9X0 Solute carrier family 2, facilitated glucose transporter member 9; Glucose transporter type 9; GLUT-9; Urate transporter from Mus musculus (Mouse) (see paper)
29% identity, 34% coverage: 56:203/441 of query aligns to 93:224/538 of Q3T9X0

query
sites
Q3T9X0

L

V

T

T

L

V

M

S

T

I

F

F

G

A

A

I

G

G

F

G

L

L

M

V

R

G

P

T

L

L

G

M

A

V

I

K

V

M

L

I

G

G

A

K

Y

F

I

L

D

-

H

-

G

G

R

R

R

K

R

S

G

T

L

L

I

V

T

N

L

N

G

G

L

F

M

A

A

I

L

S

G

A

T

A

L

L

T

L

I

M

A

A

C

C

T

S

P

L

S

R

Y

A

H

G

S

T

I

F

G

E

M

M

A

-

P

-

L

-

I

L

L

I

A

V

G

G

R

R

L

F

L

I

Q

M

G

G

F

V

S

D

A

G

G

G

V

I

E

A

L

L

G

S

G

A

V

L

S

P

V

M

Y

Y

L

L

S

N

E

E

I

I

A

S

P

P

K

K

N

E

R

I

K

R

G

G

F

-

Y

-

V

-

S

-

W

-

Q

-

S

-

G

S

S

L

Q

G

Q

Q

I

V

A

T

V

A

I

I

F

F

A

I

A

C

L

-

L

I

G

G

V

V

T

F

L

S

N

G

Q

Q

Y

L

L

L

G

G

-

L

-

P

K

E

S

L

V

L

M

G

T

R

E

E

W

S

G

T

W

W

R

-

I

-

P

P

F

Y

I

L

V

F

G

G

C

V

L

I

I

I

V

V

P

P

F

A

L

L

Sites not aligning to the query:

71 N→Q: Decreased N-glycosylation.

P17809 Solute carrier family 2, facilitated glucose transporter member 1; Glucose transporter type 1, erythrocyte/brain; GLUT-1; GT1 from Mus musculus (Mouse) (see 3 papers)
23% identity, 37% coverage: 70:232/441 of query aligns to 78:241/492 of P17809

query
sites
P17809

L

I

G

G

A

S

I

F

V

S

L

V

G

G

A

L

Y

F

I

V

D

N

H

R

H

F

G

G

R

R

R

N

G

S

L

M

L

L

I

-

T

-

L

-

G

-

L

M

M

M

A

N

L

L

G

L

T

A

L

F

T

V

I

A

A

A

C

V

T

L

P

M

S

G

Y

F

H

S

S

K

I

L

G

G

M

K

A

S

A

F

P

E

L

M

L

L

I

I

L

L

A

-

G

G

R

R

L

F

L

I

Q

I

G

G

F

V

S

Y

A

C

G

G

V

L

E

T

L

T

G

G

G

F

V

V

S

P

V

M

Y

Y

L

V

S

G

E

E

I

V

A

S

P

P

K

T

N

A

R

L

K

R

G

G

F

A

Y

L

V

G

S

T

W

L

Q

H

S

Q

G

L

S

G

Q

I

Q

V

I

V

A

G

V

I

I

L

F

I

A

A

A

Q

L

V

L

F

G

G

V

-

T

-

L

L

N

D

Q

S

Y

I

L

M

G

G

K

N

S

A

V

D

M

L

T

-

E

-

W

-

G

-

W

W

R

P

-

L

-

S

-

V

-

I

-

F

I

I

P

P

F

A

I

L

V

L

G

Q

C

C

L

I

I

L

V

L

P

P

F

F

L

C

F

P

Y

E

I

S

R

P

R

R

M

F

L

L

-

L

-

I

-

N

-

R

-

N

E

E

T

N

E

R

A

A

F

K

N

S

Q

V

R

L

K

K

H

K

R

L

P

R

S

G

M

T

S

A

E

D

I

V

T

T

R

R

S

D

V

L

Sites not aligning to the query:

45 modified: carbohydrate, N-linked (GlcNAc...) asparagine
485 P→L: Lethality immediately after birth in knockin mice; caused by creation of a dileucine internalization motif that promotes mislocalization of the protein.

6m2lA Crystal structure of plasmodium falciparum hexose transporter pfht1 bound with c3361 (see paper)
22% identity, 71% coverage: 60:371/441 of query aligns to 43:385/447 of 6m2lA

query
sites
6m2lA

T

T

F

I

G

Q

A

S

G

S

F

F

L

L

M

L

R

A

P

S

-

V

-
x
F

L

I

G

G

A

A

I

V

V

L

-

G

-

C

-

G

L

F

G

S

A

G

Y

Y

I

L

D

V

H

Q

H

F

G

G

R

R

R

L

G

S

L

L

I

I

T

I

L

Y

G

N

L

F

M

F

A

F

L

L

G

V

T

S

L

I

T

L

I

T

A

S

C

I

T

T

P

H

S

H

Y

F

H

H

S

T

I

I

G

-

M

-

A

-

P

-

L

-

I

-

L

L

A

F

G

A

R

R

L

L

Q

S

G

G

F

F

S

G

A

I

G

G

V

L

E

V

L

T

G

V

G

S

V

V

S

P

V

M

Y

Y

L

I

S

S

E

E

I

M

A

T

P

H

K

K

N

D

R

K

K

K

G

G

F

A

Y

Y

V

-

S

-

W

-

Q

-

S

G

G

V

S

M

Q

H

Q
|
Q

I

L

A

F

V

I

I

T

F

F

A

G

A

I

L

F

L

V

G

A

V

V

T

M

L

L

N

-

Q

-

Y

-

L

-

G

G

K

L

S

S

V

F

M

A

T

K

E

L

W

W

G

-

W

W

R

R

I

L

P

M

F

F

I

L

V

F

G

P

C

S

L

V

I

I

-

S

-

L

-

I

-

G

-

I

-

L

-

A

-

L

-

V

-

F

-

K

-

E

V

T

P

P

F

Y

L

F

F

L

Y

F

I

E

R

K

R

G

M

R

L

I

E

E

T

S

E

K

A

N

F

I

N

L

Q

K

R

K

K

I

H

Y

R

E

P

T

S

D

-

N

-

V

-

D

-

E

-

P

M

L

S

N

E

A

I

I

T

K

R

E

S

A

V

V

A

E

S

Q

N

N

W

E

Q

S

L

A

V

K

L

K

V

N

G

S

M

L

F

S

M

L

V

L

V

S

T

A

T

L

T

K

V

I

S

P

F

S

Y

Y

-

R

-

Y

-

V

-

I

-

L

-

G

-

C

L

L

I

L

T

S

A

G

F

L

T

Q

P

Q

T

F

Y

T

G

G

K

I

T
x
N

V

V

L

L

Q

V

F
x
S

T

N

A

S

Q

N

Q

E

-

L

-

Y

-

K

-

E

-

F

-

L

-

D

S

S

F

H

L

L

V

I

T

T

L

I

L

L

-

S

-

V

-

M

V

T

G

A

L

V

S

N

N

F

L

L

F

M

W

T

L

F

P

P

V

A

M

I

G

-

A

Y

L

I

S

V

D

E

K

K

V

L

G

G

R

R

R

K

P

T

L

L

L

W

F

G

S

C

A

V

L

G

M

V

L

L

L

V

T

A

S

Y

Y

L

P

P

S

T

L

A

-

I

-

A

S

N

W

E

L

I

V

N

A

R

H

N

P

S

S

N

F

F

A

V

H

K

L

I

V

L

E

S

V

I

E

V

-

A

-

T

-

F

-

V

L

M

W

I

L

I

S

S

F

F

M

A

Y

-

A

V

S

S

Y

Y

N

G

G

P

A

V

M

L

V
x
W

V

I

C

Y

L

L

T

H

E

E

I

M

M

F

P

P

A

S

E

E

V

I

R

K

A

D

S

S

G

A

F

A

S

S

M

L

A

A

binding (2S,3R,4S,5R,6R)-6-(hydroxymethyl)-4-undec-10-enoxy-oxane-2,3,5-triol: F54 (vs. gap), Q138 (= Q162), N265 (≠ T266), S269 (≠ F270), W366 (≠ V352)

Sites not aligning to the query:

binding (2S,3R,4S,5R,6R)-6-(hydroxymethyl)-4-undec-10-enoxy-oxane-2,3,5-triol: 25

8y66A Cryo-em structure of human urate transporter glut9 bound to inhibitor apigenin (see paper)
31% identity, 27% coverage: 83:201/441 of query aligns to 83:188/467 of 8y66A

query
sites
8y66A

G

G

R

R

R

K

R

H

G

T

L

L

I

A

T

N

L

N

G

G

L

F

M

A

A

I

L

S

G

A

T

A

L

L

T

L

I

M

A

A

C

C

T

-

P

-

S

-

Y

-

H

-

S

S

I

L

G

Q

M

A

A

G

A

A

P

F

L

E

L

M

I

L

L

I

A

V

G

G

R

R

L

F

L

I

Q

M

G

G

F

I

S

D

A

G

G

G

V

V

E

A

L
|
L

G

S

G

V

V

L

S

P

V

M

Y

Y

L

L

S

S

E

E

I

I

A

S

P

P

K

K

N

E

R

I

K

R

G

G

F

-

Y

-

V

-

S

-

W

-

Q

-

S

-

G

S

S

L

Q

G

Q

Q

I

V

A

T

V

A

I

I

F

F

A

I

A
x
C

L

-

L

I

G

G

V

V

T

F

L

T

N

G

Q

Q

Y

L

L

L

G

G

-

L

-

P

K

E

S

L

V

L

M

G

T

K

E

E

W

S

G

T

W

W

R

-

I

-

P

P

F

Y

I

L

V

F

G

G

C

V

L

I

I

V

V

V

P

P

binding 5,7-dihydroxy-2-(4-hydroxyphenyl)-4H-chromen-4-one: L129 (= L134), C157 (≠ A169)

Sites not aligning to the query:

binding 5,7-dihydroxy-2-(4-hydroxyphenyl)-4H-chromen-4-one: 274, 382, 405

8y65A Cryo-em structure of human urate transporter glut9 bound to substrate urate (see paper)
31% identity, 27% coverage: 83:201/441 of query aligns to 83:188/467 of 8y65A

query
sites
8y65A

G

G

R

R

R

K

R

H

G

T

L

L

I

A

T

N

L

N

G

G

L

F

M

A

A

I

L

S

G

A

T

A

L

L

T

L

I

M

A

A

C

C

T

-

P

-

S

-

Y

-

H

-

S

S

I

L

G

Q

M

A

A

G

A

A

P

F

L

E

L

M

I

L

L

I

A

V

G

G

R

R

L

F

L

I

Q

M

G

G

F

I

S

D

A

G

G

G

V

V

E

A

L

L

G

S

G

V

V

L

S

P

V

M

Y

Y

L

L

S

S

E

E

I

I

A

S

P

P

K

K

N

E

R

I

K

R

G

G

F

-

Y

-

V

-

S

-

W

-

Q

-

S

-

G

S

S

L

Q

G

Q

Q

I

V

A

T

V

A

I

I

F

F

A

I

A

C

L

-

L

I

G

G

V

V

T

F

L

T

N

G

Q

Q

Y

L

L

L

G

G

-

L

-

P

K

E

S

L

V

L

M

G

T

K

E

E

W

S

G

T

W

W

R

-

I

-

P

P

F

Y

I

L

V

F

G

G

C

V

L

I

I

V

V

V

P

P

Sites not aligning to the query:

binding uric acid: 274, 279, 280, 283

Q9NRM0 Solute carrier family 2, facilitated glucose transporter member 9; Glucose transporter type 9; GLUT-9; Urate transporter from Homo sapiens (Human) (see 13 papers)
31% identity, 27% coverage: 83:201/441 of query aligns to 136:241/540 of Q9NRM0

query
sites
Q9NRM0

G

G

R

R

R

K

R

H

G

T

L

L

I

A

T

N

L

N

G

G

L

F

M

A

A

I

L

S

G

A

T

A

L

L

T

L

I

M

A

A

C
|
C

T

-

P

-

S

-

Y

-

H

-

S

S

I

L

G

Q

M

A

A

G

A

A

P

F

L

E

L

M

I

L

L

I

A
x
V

G

G

R
|
R

L

F

L

I

Q

M

G

G

F

I

S

D

A

G

G

G

V

V

E

A

L

L

G

S

G

V

V

L

S

P

V

M

Y

Y

L

L

S

S

E
|
E

I

I

A

S

P

P

K

K

N

E

R

I

K
x
R

G

G

F

-

Y

-

V

-

S

-

W

-

Q

-

S

-

G

S

S

L

Q

G

Q

Q

I

V

A

T

V

A

I

I

F

F

A

I

A
x
C

L

-

L

I

G

G

V

V

T

F

L

T

N
x
G

Q

Q

Y

L

L

L

G

G

-

L

-

P

K

E

S

L

V

L

M

G

T

K

E

E

W

S

G

T

W

W

R

-

I

-

P

P

F

Y

I

L

V

F

G

G

C

V

L

I

I

V

V

V

P

P

C157 (= C104) mutation to V: No effect on fructose transport. Increased urate binding affinity and decreased urate transport capacity.
V169 (≠ A121) to M: no effect on urate transport activity; dbSNP:rs144196049
R171 (= R123) to C: in RHUC2; uncertain significance; according to PubMed:21810765 the variant results in markedly reduced urate uptake, however according to PubMed:29967582 urate transport activity is not affected; no effect on Vmax; no effect on Km for urate; decreased protein expression; no effect on glucose transport; dbSNP:rs776127501
E191 (= E143) to D: in dbSNP:rs376990050
R198 (≠ K150) to C: in RHUC2; markedly reduced urate transport activity; decreased Vmax; decreased urate uptake; no effect on protein expression; no effect on glucose transport; dbSNP:rs121908322
C210 (≠ A169) mutation to F: Decreased urate uptake. Decreased Vmax for urate transport. Has no effect on glucose transport.; mutation to T: Decreased fructose transport. Higher affinity and lower transport capacity for urate.
G216 (≠ N176) to R: in RHUC2; decreased protein expression; decreased urate uptake; no effect on glucose transport; dbSNP:rs561633150

Sites not aligning to the query:

22 natural variant: S -> N
25 G → R: no effect on urate transport activity; dbSNP:rs2276961
75 L → R: in RHUC2; reduced urate transport activity; decreased protein expression; decreased urate uptake; no effect on glucose transport; dbSNP:rs863225072
125 T → M: in RHUC2; markedly reduced urate transport activity; decreased protein expression; decreased urate uptake; no effect on glucose transport; dbSNP:rs181509591
275 T → M: no effect on urate transport activity; dbSNP:rs112404957
281 D → H: no effect on urate transport activity; dbSNP:rs73225891
282 V → I: no effect on urate transport activity; dbSNP:rs16890979
294 R → H: no effect on urate transport activity; no effect on protein expression; no effect on glucose transport; dbSNP:rs3733591
300 R → H: in dbSNP:rs145688560
326 C→G: No effect on urate and fructose transport.
330 C→S: Increased fructose transport. Highly reduced urate transport.
332 L→V: Increased fructose binding affinity and decreased fructose transport capacity.
333 N → S: in RHUC2; decreased urate uptake; increased Km for urate; no effect on protein expression; no effect on glucose transport; dbSNP:rs753482595
350 P → L: no effect on urate transport activity; no effect on urate transport; no effect on protein expression; no effect on glucose transport; dbSNP:rs2280205
380 R → W: in RHUC2; markedly reduced urate transport activity; decreased Vmax; no effect on protein expression; no effect on glucose transport; dbSNP:rs121908321
412 P → R: in RHUC2; uncertain significance; according to PubMed:18701466 the variant results in decreased urate uptake, however according to PubMed:22132964 and PubMed:29967582 urate transport activity is not affected; no effect on Vmax for urate uptake; no effect on affinity for urate; no effect on protein expression; no effect on localization to plasma membrane; no effect on glucose transport; dbSNP:rs121908323
427 C→A: No effect on fructose transport. Higher affinity and lower transport capacity for urate.
480 C→S: No effect on urate and fructose transport.
488 C→L: No effect on fructose transport. Highly reduced urate transport.

Query Sequence

>WP_008814175.1 NCBI__GCF_001655005.1:WP_008814175.1
MTSSASAPTQANARTVFRVTSGNFLEMYDFMVFGYYASAIADTFFPTQSPFASLMLTLMT
FGAGFLMRPLGAIVLGAYIDHHGRRRGLLITLGLMALGTLTIACTPSYHSIGMAAPLLIL
AGRLLQGFSAGVELGGVSVYLSEIAPKNRKGFYVSWQSGSQQIAVIFAALLGVTLNQYLG
KSVMTEWGWRIPFIVGCLIVPFLFYIRRMLEETEAFNQRKHRPSMSEITRSVASNWQLVL
VGMFMVVTTTVSFYLITAFTPTYGKTVLQFTAQQSFLVTLLVGLSNLFWLPVMGALSDKV
GRRPLLLLFSALMLLTSYPSLSWLVAHPSFAHLVEVELWLSFMYASYNGAMVVCLTEIMP
AEVRASGFSMAYSLATAIFGGFTPAISSYLIHATGDKAMPGLWLSAAAACGLVAAIALPA
IQKLNHQRTETAPNHGVATPK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory