SitesBLAST
Comparing WP_008859401.1 NCBI__GCF_000242435.1:WP_008859401.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
1wwkA Crystal structure of phosphoglycerate dehydrogenase from pyrococcus horikoshii ot3
39% identity, 88% coverage: 32:307/314 of query aligns to 24:304/304 of 1wwkA
- active site: S96 (= S104), R230 (= R232), D254 (= D256), E259 (≠ P262), H278 (= H281)
- binding nicotinamide-adenine-dinucleotide: V100 (= V108), G146 (= G153), F147 (≠ T154), G148 (= G155), R149 (≠ A156), I150 (= I157), Y168 (= Y175), D169 (≠ N176), P170 (≠ R177), V201 (≠ L203), P202 (= P204), T207 (= T209), T228 (= T230), S229 (≠ A231), D254 (= D256), H278 (= H281), G280 (= G283)
3kb6B Crystal structure of d-lactate dehydrogenase from aquifex aeolicus complexed with NAD and lactic acid (see paper)
35% identity, 85% coverage: 46:312/314 of query aligns to 38:325/334 of 3kb6B
- active site: S97 (= S104), R231 (= R232), D255 (= D256), E260 (≠ M260), H294 (= H281)
- binding lactic acid: F49 (≠ A57), S72 (≠ F79), V73 (≠ T80), G74 (= G81), Y96 (= Y103), R231 (= R232), H294 (= H281)
- binding nicotinamide-adenine-dinucleotide: V73 (≠ T80), Y96 (= Y103), V101 (= V108), G150 (= G155), R151 (≠ A156), I152 (= I157), D171 (≠ N176), V172 (≠ R177), P203 (= P204), T229 (= T230), A230 (= A231), R231 (= R232), H294 (= H281), A296 (≠ G283), Y297 (≠ F284)
6p2iA Acyclic imino acid reductase (bsp5) in complex with NADPH and d-arg (see paper)
35% identity, 87% coverage: 40:313/314 of query aligns to 34:306/307 of 6p2iA
- binding d-arginine: E51 (≠ A57), T73 (≠ F79), T74 (= T80), S75 (≠ G81), Y97 (= Y103), W277 (≠ F284)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: S98 (= S104), V102 (= V108), G149 (= G153), I150 (≠ T154), G151 (= G155), Q152 (≠ A156), I153 (= I157), N172 (= N176), K173 (≠ R177), S174 (= S178), R176 (≠ S180), H199 (= H202), I200 (≠ L203), P201 (= P204), T206 (= T209), T227 (= T230), C228 (≠ A231), W277 (≠ F284)
5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
35% identity, 85% coverage: 41:308/314 of query aligns to 36:315/334 of 5aovA
- active site: L100 (≠ S104), R241 (= R232), D265 (= D256), E270 (= E261), H288 (= H281)
- binding glyoxylic acid: M52 (≠ I56), L53 (≠ A57), L53 (≠ A57), Y74 (≠ A78), A75 (≠ F79), V76 (≠ T80), G77 (= G81), R241 (= R232), H288 (= H281)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (≠ T80), T104 (≠ V108), F158 (≠ T154), G159 (= G155), R160 (≠ A156), I161 (= I157), S180 (≠ N176), R181 (= R177), A211 (≠ H202), V212 (≠ L203), P213 (= P204), T218 (= T209), I239 (≠ T230), A240 (= A231), R241 (= R232), H288 (= H281), G290 (= G283)
4zgsA Identification of the pyruvate reductase of chlamydomonas reinhardtii (see paper)
35% identity, 75% coverage: 70:305/314 of query aligns to 77:335/346 of 4zgsA
- active site: S111 (= S104), R244 (= R232), D268 (= D256), E273 (= E261), H311 (= H281)
- binding nicotinamide-adenine-dinucleotide: Y110 (= Y103), G163 (= G155), A164 (= A156), I165 (= I157), D184 (vs. gap), C215 (≠ L203), P216 (= P204), L218 (≠ T206), S220 (≠ D208), T221 (= T209), S243 (≠ A231), H311 (= H281), F314 (= F284)
5z20F The ternary structure of d-lactate dehydrogenase from pseudomonas aeruginosa with nadh and oxamate (see paper)
34% identity, 83% coverage: 53:313/314 of query aligns to 55:334/336 of 5z20F
- active site: S108 (= S104), R241 (= R232), D265 (= D256), E270 (= E261), H302 (= H281)
- binding 1,4-dihydronicotinamide adenine dinucleotide: Y107 (= Y103), G160 (= G155), Q161 (≠ A156), I162 (= I157), Y180 (= Y175), D181 (≠ N176), P182 (≠ S178), C212 (≠ L203), P213 (= P204), T218 (= T209), T239 (= T230), G240 (≠ A231), R241 (= R232), H302 (= H281), A304 (≠ G283)
2eklA Structure of st1218 protein from sulfolobus tokodaii
32% identity, 90% coverage: 18:301/314 of query aligns to 15:302/312 of 2eklA
- active site: S100 (= S104), R232 (= R232), D256 (= D256), E261 (= E261), H282 (= H281)
- binding nicotinamide-adenine-dinucleotide: I76 (≠ T80), S100 (= S104), G148 (= G153), G150 (= G155), R151 (≠ A156), I152 (= I157), Y170 (= Y175), D171 (≠ N176), I172 (vs. gap), L173 (vs. gap), H202 (= H202), V203 (≠ L203), T204 (≠ P204), I212 (≠ F212), T230 (= T230), S231 (≠ A231), D256 (= D256), G284 (= G283)
6ttbA Crystal structure of NAD-dependent formate dehydrogenase from staphylococcus aureus in complex with NAD
34% identity, 93% coverage: 24:314/314 of query aligns to 31:328/331 of 6ttbA
- binding nicotinamide-adenine-dinucleotide: V87 (≠ T80), N111 (≠ S104), V115 (= V108), F162 (≠ I152), G165 (= G155), R166 (≠ A156), I167 (= I157), Y185 (= Y175), D186 (≠ N176), P187 (vs. gap), H214 (= H202), A215 (≠ L203), P216 (= P204), T221 (= T209), T242 (= T230), A243 (= A231), R244 (= R232), H292 (= H281), S294 (≠ G283), G295 (≠ F284)
1dxyA Structure of d-2-hydroxyisocaproate dehydrogenase (see paper)
34% identity, 75% coverage: 69:305/314 of query aligns to 66:319/330 of 1dxyA
- active site: S101 (= S104), R234 (= R232), D258 (= D256), E263 (= E261), H295 (= H281)
- binding 2-oxo-4-methylpentanoic acid: V77 (≠ T80), Y100 (= Y103), Y298 (≠ F284)
- binding nicotinamide-adenine-dinucleotide: Y100 (= Y103), G152 (= G153), G154 (= G155), H155 (≠ A156), I156 (= I157), Y174 (= Y175), D175 (≠ N176), P176 (vs. gap), H204 (= H202), V205 (≠ L203), P206 (= P204), N211 (≠ T209), T232 (= T230), A233 (= A231), R234 (= R232), H295 (= H281), Y298 (≠ F284)
P17584 D-2-hydroxyisocaproate dehydrogenase; D-HICDH; EC 1.1.1.- from Lacticaseibacillus paracasei (Lactobacillus paracasei) (see paper)
34% identity, 75% coverage: 69:305/314 of query aligns to 66:319/333 of P17584
- HI 155:156 (≠ AI 156:157) binding NAD(+)
- D175 (≠ N176) binding NAD(+)
- V205 (≠ L203) binding NAD(+)
- N211 (≠ T209) binding NAD(+)
- TAR 232:234 (= TAR 230:232) binding NAD(+)
- D258 (= D256) binding NAD(+)
2yq5C Crystal structure of d-isomer specific 2-hydroxyacid dehydrogenase from lactobacillus delbrueckii ssp. Bulgaricus: NAD complexed form (see paper)
34% identity, 81% coverage: 60:313/314 of query aligns to 55:330/331 of 2yq5C
- active site: S102 (= S104), R236 (= R232), D260 (= D256), E265 (= E261), H297 (= H281)
- binding nicotinamide-adenine-dinucleotide: Y101 (= Y103), I106 (≠ V108), V155 (≠ T154), G156 (= G155), H157 (≠ A156), I158 (= I157), Y176 (= Y175), D177 (≠ N176), V178 (≠ R177), H206 (= H202), T207 (≠ L203), P208 (= P204), A235 (= A231), R236 (= R232), H297 (= H281), F300 (= F284)
6biiA Crystal structure of pyrococcus yayanosii glyoxylate hydroxypyruvate reductase in complex with NADP and malonate (re-refinement of 5aow) (see paper)
34% identity, 85% coverage: 41:308/314 of query aligns to 35:314/332 of 6biiA
- active site: L99 (≠ S104), R240 (= R232), D264 (= D256), E269 (= E261), H287 (= H281)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V75 (≠ T80), T103 (≠ V108), G156 (= G153), F157 (≠ T154), G158 (= G155), R159 (≠ A156), I160 (= I157), A179 (≠ N176), R180 (= R177), S181 (= S178), K183 (≠ S180), V211 (≠ L203), P212 (= P204), E216 (≠ D208), T217 (= T209), V238 (≠ T230), A239 (= A231), R240 (= R232), D264 (= D256), H287 (= H281), G289 (= G283)
4xkjA A novel d-lactate dehydrogenase from sporolactobacillus sp
38% identity, 75% coverage: 60:296/314 of query aligns to 59:310/332 of 4xkjA
- active site: S102 (= S104), R234 (= R232), D258 (= D256), E263 (vs. gap), H295 (= H281)
- binding nicotinamide-adenine-dinucleotide: Y101 (= Y103), V106 (= V108), G152 (= G153), G154 (= G155), R155 (≠ A156), I156 (= I157), D175 (≠ N176), I176 (vs. gap), R179 (= R177), H204 (= H202), V205 (≠ L203), P206 (= P204), T211 (= T209), A232 (≠ T230), R234 (= R232), H295 (= H281), G297 (= G283), F298 (= F284)
O43175 D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; Malate dehydrogenase; EC 1.1.1.95; EC 1.1.1.399; EC 1.1.1.37 from Homo sapiens (Human) (see 3 papers)
32% identity, 82% coverage: 53:308/314 of query aligns to 51:310/533 of O43175
- T78 (= T80) binding NAD(+)
- R135 (≠ G136) to W: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606949
- RI 155:156 (≠ AI 156:157) binding NAD(+)
- D175 (≠ N176) binding NAD(+)
- T207 (≠ L203) binding NAD(+)
- CAR 234:236 (≠ TAR 230:232) binding NAD(+)
- D260 (= D256) binding NAD(+)
- V261 (= V257) to M: in PHGDHD; results in a four-fold decrease in substrate affinity and a slight increase in maximal enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs267606947
- HLGA 283:286 (≠ HVGF 281:284) binding NAD(+)
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
- 373 A → T: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs201553627
- 377 G → S: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606948
- 425 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907988
- 490 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907987
7arzA Ternary complex of NAD-dependent formate dehydrogenase from physcomitrium patens
34% identity, 78% coverage: 61:306/314 of query aligns to 85:340/361 of 7arzA
- binding azide ion: L99 (= L75), V100 (≠ C76), T101 (≠ V77), G103 (≠ F79), V104 (≠ T80), R267 (= R232), H315 (= H281)
- binding nicotinamide-adenine-dinucleotide: V104 (≠ T80), N128 (≠ S104), V132 (= V108), G182 (= G155), R183 (≠ A156), I184 (= I157), D204 (vs. gap), R205 (vs. gap), T238 (≠