SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_009064810.1 NCBI__GCF_000016505.1:WP_009064810.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 5 hits to proteins with known functional sites (download)

4gyfA Crystal structure of histidinol phosphate phosphatase (hisk) from lactococcus lactis subsp. Lactis il1403 complexed with zn, l- histidinol and phosphate
33% identity, 96% coverage: 4:270/277 of query aligns to 6:260/263 of 4gyfA

query
sites
4gyfA

D

D

Y

Y

H
|
H

V

F

H
|
H

T

S

E

H

F

F

S

S

D

A

D
|
D

S

S

V

E

Y

E

E

L

M

P

E

R

E

K

V

H

V

V

K

T

D

E

A

A

I

I

Q

A

I
x
H

K

G

M

L

E

E

I

I

C

C

F

F

T

T

D

E

H
|
H

V

R

D

D

Y

F

G

-

V

-

K

-

L

-

D

-

W

-

N

-

D

-

K

-

E

-

I

-

Q

-

Y

Y

R

F

D

P

G

G

M

M

P

D

M

F

A

S

N

-

V

L

Y

N

Y

L

P

P

E

E

Y

Y

F

F

E

Q

T

E

I

I

K

N

E

Q

L

L

Q

Q

Y

A

L

E

Y

F

G

K

D

D

L

K

I

I

A

K

I

I

K

K

K

I

G

G

M

L

E
|
E

F

M

G

G

V

I

Q

D

V

L

H

R

T

F

I

K

S

S

Q

E

Y

I

E

N

K

Q

L

F

F

I

V

D

K

S

Y

A

P

P

F

F

D

D

F

F

I

V

I

I

L

A

S

S

V

V

H
|
H

Q

E

I

I

D

G

N

D

L

I

E
|
E

L

V

W

Y

T

D

-

G

Q

T

D

E

F

F

Q

Y

K

L

G

Q

K

K

S

T

Q

K

K

E

E

E

Y

A

N

Q

E

R

K

E

Y

Y

Y

L

Q

L

E

A

L

C

L

L

E

D

I

V

V

V

K

Q

Q

N

Y

F

K

E

N

N

Y

Y

S

N

V

S

L

F

G

G

H
|
H

L

L

D

D

I
x
Y

I

V

I

A

R

R

Y
|
Y

D

G

K

P

-

Y

-

T

N

D

G

K

T

S

Y

I

P

K

F

F

E

A

K

E

I

N

S

R

D

E

I

I

V

L

N

F

E

E

I

I

L

L

K

R

I

A

V

L

I

A

R

S

D

K

G

E

K

K

G

A

I

L

E

E

V

I

N

N

T

T

S

R

S

L

Y

F

R

D

Y

D

G

P

L

K

T

T

D

E

M

Q

T

F

P

Y

S

S

K

-

D

D

I

L

L

L

T

I

L

N

Y

F

K

K

K

R

L

L

G

G

E

K

I

F

I

I

T

T

I

L

G

G

S

T

D
|
D

S

S

H
|
H

K

I

K

A

G

K

Q

R

L

D

G

W

A

L

H

S

I

I

E
x
H

N

K

T

A

K

R

K

T

V

L

L

I

F

K

D

K

M

A

G

G

F

F

R

H

Y

E

H

L

C

A

T

T

Y

F

E

S

N

G

M

M

R

K

binding L-histidinol: H31 (≠ I29), E114 (= E126), Y156 (≠ I167), Y160 (= Y171)
binding zinc ion: H8 (= H6), H10 (= H8), D16 (= D14), H31 (≠ I29), H41 (= H39), E80 (= E92), E80 (= E92), H108 (= H120), H153 (= H164), D227 (= D237), H229 (= H239), H239 (≠ E249)

Sites not aligning to the query:

binding L-histidinol: 4

4gk8A Crystal structure of histidinol phosphate phosphatase (hisk) from lactococcus lactis subsp. Lactis il1403 complexed with zn and l- histidinol arsenate (see paper)
33% identity, 96% coverage: 4:270/277 of query aligns to 6:260/263 of 4gk8A

query
sites
4gk8A

D

D

Y

Y

H
|
H

V

F

H
|
H

T

S

E

H

F

F

S

S

D

A

D
|
D

S

S

V

E

Y

E

E

L

M

P

E

R

E

K

V

H

V

V

K

T

D

E

A

A

I

I

Q

A

I
x
H

K

G

M

L

E

E

I

I

C

C

F

F

T

T

D

E

H
|
H

V

R

D

D

Y

F

G

-

V

-

K

-

L

-

D

-

W

-

N

-

D

-

K

-

E

-

I

-

Q

-

Y

Y

R

F

D

P

G

G

M

M

P

D

M

F

A

S

N

-

V

L

Y

N

Y

L

P

P

E

E

Y

Y

F

F

E

Q

T

E

I

I

K

N

E

Q

L

L

Q

Q

Y

A

L

E

Y

F

G

K

D

D

L

K

I

I

A

K

I

I

K

K

K

I

G

G

M

L

E
|
E

F

M

G

G

V

I

Q

D

V

L

H

R

T

F

I

K

S

S

Q

E

Y

I

E

N

K

Q

L

F

F

I

V

D

K

S

Y

A

P

P

F

F

D

D

F

F

I

V

I

I

L

A

S

S

V

V

H
|
H

Q

E

I

I

D

G

N

D

L

I

E
|
E

L

V

W

Y

T

D

-

G

Q

T

D

E

F

F

Q

Y

K

L

G

Q

K

K

S

T

Q

K

K

E

E

E

Y

A

N

Q

E

R

K

E

Y

Y

Y

L

Q

L

E

A

L

C

L

L

E

D

I

V

V

V

K

Q

Q

N

Y

F

K

E

N

N

Y

Y

S

N

V

S

L

F

G

G

H
|
H

L

L

D

D

I
x
Y

I

V

I

A

R
|
R

Y

Y

D

G

K

P

-

Y

-

T

N

D

G

K

T

S

Y

I

P

K

F

F

E

A

K

E

I

N

S

R

D

E

I

I

V

L

N

F

E

E

I

I

L

L

K

R

I

A

V

L

I

A

R

S

D

K

G

E

K

K

G

A

I

L

E

E

V

I

N

N

T

T

S

R

S

L

Y

F

R

D

Y

D

G

P

L

K

T

T

D

E

M

Q

T

F

P

Y

S

S

K

-

D

D

I

L

L

L

T

I

L

N

Y

F

K

K

K

R

L

L

G

G

E

K

I

F

I

I

T

T

I

L

G

G

S

T

D
|
D

S

S

H
|
H

K

I

K

A

G

K

Q

R

L

D

G

W

A

L

H

S

I

I

E
x
H

N

K

T

A

K

R

K

T

V

L

L

I

F

K

D

K

M

A

G

G

F

F

R

H

Y

E

H

L

C

A

T

T

Y

F

E

S

N

G

M

M

R

K

binding {[(2S)-2-amino-3-(1H-imidazol-5-yl)propyl]oxy}(trihydroxy)-lambda~5~-arsanyl: H10 (= H8), D16 (= D14), H41 (= H39), H108 (= H120), E114 (= E126), Y156 (≠ I167), R159 (= R170), D227 (= D237), H229 (= H239)
binding zinc ion: H8 (= H6), H10 (= H8), D16 (= D14), H31 (≠ I29), H41 (= H39), E80 (= E92), E80 (= E92), H108 (= H120), H153 (= H164), D227 (= D237), H229 (= H239), H239 (≠ E249)

4gc3A Crystal structure of l-histidinol phosphate phosphatase (hisk) from lactococcus lactis subsp. Lactis il1403 complexed with zn and sulfate (see paper)
33% identity, 96% coverage: 4:270/277 of query aligns to 6:260/263 of 4gc3A

query
sites
4gc3A

D

D

Y

Y

H
|
H

V

F

H
|
H

T

S

E

H

F

F

S

S

D

A

D
|
D

S

S

V

E

Y

E

E

L

M

P

E

R

E

K

V

H

V

V

K

T

D

E

A

A

I

I

Q

A

I

H

K

G

M

L

E

E

I

I

C

C

F

F

T

T

D

E

H
|
H

V

R

D

D

Y

F

G

-

V

-

K

-

L

-

D

-

W

-

N

-

D

-

K

-

E

-

I

-

Q

-

Y

Y

R

F

D

P

G

G

M

M

P

D

M

F

A

S

N

-

V

L

Y

N

Y

L

P

P

E

E

Y

Y

F

F

E

Q

T

E

I

I

K

N

E

Q

L

L

Q

Q

Y

A

L

E

Y

F

G

K

D

D

L

K

I

I

A

K

I

I

K

K

K

I

G

G

M

L

E
|
E

F

M

G

G

V

I

Q

D

V

L

H

R

T

F

I

K

S

S

Q

E

Y

I

E

N

K

Q

L

F

F

I

V

D

K

S

Y

A

P

P

F

F

D

D

F

F

I

V

I

I

L

A

S

S

V

V

H
|
H

Q

E

I

I

D

G

N

D

L

I

E

E

L

V

W

Y

T

D

-

G

Q

T

D

E

F

F

Q

Y

K

L

G

Q

K

K

S

T

Q

K

K

E

E

E

Y

A

N

Q

E

R

K

E

Y

Y

Y

L

Q

L

E

A

L

C

L

L

E

D

I

V

V

V

K

Q

Q

N

Y

F

K

E

N

N

Y

Y

S

N

V

S

L

F

G

G

H
|
H

L

L

D

D

I
x
Y

I

V

I

A

R
|
R

Y

Y

D

G

K

P

-

Y

-

T

N

D

G

K

T

S

Y

I

P

K

F

F

E

A

K

E

I

N

S

R

D

E

I

I

V

L

N

F

E

E

I

I

L

L

K

R

I

A

V

L

I

A

R

S

D

K

G

E

K

K

G

A

I

L

E

E

V

I

N

N

T

T

S

R

S

L

Y

F

R

D

Y

D

G

P

L

K

T

T

D

E

M

Q

T

F

P

Y

S

S

K

-

D

D

I

L

L

L

T

I

L

N

Y

F

K

K

K

R

L

L

G

G

E

K

I

F

I

I

T

T

I

L

G

G

S

T

D
|
D

S

S

H
|
H

K

I

K

A

G

K

Q

R

L

D

G

W

A

L

H

S

I

I

E
x
H

N

K

T

A

K
x
R

K

T

V

L

L

I

F

K

D

K

M

A

G

G

F

F

R

H

Y

E

H

L

C

A

T

T

Y

F

E

S

N

G

M

M

R

K

binding sulfate ion: H41 (= H39), Y156 (≠ I167), R159 (= R170), D227 (= D237), H229 (= H239), H239 (≠ E249), R242 (≠ K252)
binding zinc ion: H8 (= H6), H10 (= H8), D16 (= D14), H41 (= H39), E80 (= E92), E80 (= E92), H108 (= H120), H153 (= H164), D227 (= D237), H229 (= H239)

2yz5A Histidinol phosphate phosphatase complexed with phosphate (see paper)
26% identity, 93% coverage: 4:261/277 of query aligns to 3:248/263 of 2yz5A

query
sites
2yz5A

D

D

Y

S

H
|
H

V

V

H
|
H

T

T

E

P

F

L

S

C

D

G

D
x
H

S

A

V

E

Y

G

E

H

M

P

E

E

E

A

V

Y

V

L

K

E

D

E

A

A

I

R

Q

A

I

K

K

G

M

L

E

K

E

G

I

V

C

V

F

F

T

T

D

D

H
|
H

V

S

D

P

Y

M

G

P

V

-

K

-

L

-

D

P

W

W

N

Y

D

D

K

P

K

E

E

S

I

R

Q

M

Y

R

R

L

D

E

G

A

M

L

P

P

M

-

A

-

N

-

V

-

Y

F

Y

Y

P

L

E

L

Y

A

F

L

E

E

T

R

I

V

K

R

E

E

-

R

L

A

Q

Q

Y

D

L

L

Y

Y

G

-

D

-

L

-

I

-

A

-

I

V

K

G

K

I

G

G

M

L

E
|
E

F

A

G

D

V

F

Q

H

V

P

H

G

T

T

I

E

S

G

Q

F

Y

L

E

A

K

Q

L

L

F

L

V

R

K

R

Y

Y

P

P

F

F

D

D

F

Y

I

V

I

I

L

G

S

S

V

V

H
|
H

Q

Y

I

L

D

G

N

A

L

W

E

P

L

L

W

D

T

H

Q

P

D

D

F

H

Q

Q

K

E

-

E

-

Y

-

A

G

W

K

R

S

D

Q

L

K

K

E

E

Y

V

N

F

E

R

K

A

Y

Y

Y

F

Q

Q

E

E

L

-

L

V

E

E

I

K

V

A

K

A

Q

R

Y

S

K

G

N

L

Y

F

S

H

V

A

L

I

G

G

H
|
H

L

L

D

D

I

L

I

P

I

K

R

K

Y

F

D

G

K

H

N

R

G

-

T

-

Y

L

P

P

F

E

E

E

K

A

I

L

S

L

D

E

I

L

V

A

N

E

E

P

I

A

L

L

K

R

I

A

V

V

I

A

R

E

D

A

G

G

K

L

G

F

I

L

E

D

V

V

N

N

T

T

S

A

S

G

Y

L

R

R

Y

R

G

P

L

A

T

K

D

E

M

V

T

Y

P

P

S

A

K

P

D

A

I

L

L

L

T

R

L

R

Y

A

K

R

K

E

L

L

G

G

G

I

E

G

I

L

I

V

T

-

I

L

G

G

S

S

D
|
D

S

A

H
|
H

K

R

K

P

G

E

Q

E

L

V

G

G

A

F

H

A

I

F

E

P

N

E

T

V

K

Q

K

A

V

L

L

L

F

A

D

G

M

L

G

G

F

F

R

R

binding fe (iii) ion: H5 (= H6), H7 (= H8), E80 (= E92), E80 (= E92), H108 (= H120), H154 (= H164), D224 (= D237)
binding phosphate ion: H7 (= H8), H38 (= H39), H226 (= H239)
binding zinc ion: H13 (≠ D14), H38 (= H39), H226 (= H239)

2yxoB Histidinol phosphate phosphatase complexed with sulfate (see paper)
26% identity, 93% coverage: 4:261/277 of query aligns to 3:248/265 of 2yxoB

query
sites
2yxoB

D

D

Y

S

H
|
H

V

V

H
|
H

T

T

E

P

F

L

S

C

D

G

D
x
H

S

A

V

E

Y

G

E

H

M

P

E

E

E

A

V

Y

V

L

K

E

D

E

A

A

I

R

Q

A

I

K

K

G

M

L

E

K

E

G

I

V

C

V

F

F

T

T

D

D

H
|
H

V

S

D

P

Y

M

G

P

V

-

K

-

L

-

D

P

W

W

N

Y

D

D

K

P

K

E

E

S

I

R

Q

M

Y

R

R

L

D

E

G

A

M

L

P

P

M

-

A

-

N

-

V

-

Y

F

Y

Y

P

L

E

L

Y

A

F

L

E

E

T

R

I

V

K

R

E

E

-

R

L

A

Q

Q

Y

D

L

L

Y

Y

G

-

D

-

L

-

I

-

A

-

I

V

K

G

K

I

G

G

M

L

E
|
E

F

A

G

D

V

F

Q

H

V

P

H

G

T

T

I

E

S

G

Q

F

Y

L

E

A

K

Q

L

L

F

L

V

R

K

R

Y

Y

P

P

F

F

D

D

F

Y

I

V

I

I

L

G

S

S

V

V

H
|
H

Q

Y

I

L

D

G

N

A

L

W

E

P

L

L

W

D

T

H

Q

P

D

D

F

H

Q

Q

K

E

-

E

-

Y

-

A

G

W

K

R

S

D

Q

L

K

K

E

E

Y

V

N

F

E

R

K

A

Y

Y

Y

F

Q

Q

E

E

L

-

L

V

E

E

I

K

V

A

K

A

Q

R

Y

S

K

G

N

L

Y

F

S

H

V

A

L

I

G

G

H
|
H

L

L

D

D

I

L

I

P

I

K

R

K

Y

F

D

G

K

H

N

R

G

-

T

-

Y

L

P

P

F

E

E

E

K

A

I

L

S

L

D

E

I

L

V

A

N

E

E

P

I

A

L

L

K

R

I

A

V

V

I

A

R

E

D

A

G

G

K

L

G

F

I

L

E

D

V

V

N

N

T

T

S

A

S

G

Y

L

R

R

Y

R

G

P

L

A

T

K

D

E

M

V

T

Y

P

P

S

A

K

P

D

A

I

L

L

L

T

R

L

R

Y

A

K

R

K

E

L

L

G

G

G

I

E

G

I

L

I

V

T

-

I

L

G

G

S

S

D
|
D

S

A

H
|
H

K

R

K

P

G

E

Q

E

L

V

G

G

A

F

H

A

I

F

E

P

N

E

T

V

K

Q

K

A

V

L

L

L

F

A

D

G

M

L

G

G

F

F

R

R

binding fe (iii) ion: H5 (= H6), H7 (= H8), H13 (≠ D14), H38 (= H39), E80 (= E92), D224 (= D237), H226 (= H239)
binding sulfate ion: H7 (= H8), H38 (= H39), H108 (= H120), H226 (= H239)
binding zinc ion: E80 (= E92), H108 (= H120), H154 (= H164)

Query Sequence

>WP_009064810.1 NCBI__GCF_000016505.1:WP_009064810.1
MFADYHVHTEFSDDSVYEMEEVVKDAIQIKMEEICFTDHVDYGVKLDWNDKKEIQYRDGM
PMANVYYPEYFETIKELQYLYGDLIAIKKGMEFGVQVHTISQYEKLFVKYPFDFIILSVH
QIDNLELWTQDFQKGKSQKEYNEKYYQELLEIVKQYKNYSVLGHLDIIIRYDKNGTYPFE
KISDIVNEILKIVIRDGKGIEVNTSSYRYGLTDMTPSKDILTLYKKLGGEIITIGSDSHK
KGQLGAHIENTKKVLFDMGFRYHCTYENMRPHFHKLI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory