SitesBLAST

K92 (= K88) mutation to S: Complete loss of dehydrogenase activity.
K128 (= K124) mutation to H: Reduces catalytic activity and increases pH optima for activity. Increases relative activity with amino acid substrates other than glutamate, especially L-norvaline.; mutation to R: Reduced catalytic activity and increases pH optima for activity. NADP-specific glutamate dehydrogenase.

1bgvA Glutamate dehydrogenase (see paper)
54% identity, 99% coverage: 5:444/445 of query aligns to 6:447/449 of 1bgvA

query
sites
1bgvA

K

R

V

V

L

I

E

A

D

E

L

V

K

E

R

K

F

Y

P

A

N

D

E

E

P

P

E

E

Y

F

H

V

Q

Q

A

T

V

V

E

E

V

V

L

L

S

S

T

S

I

L

E

G

E

P

E

V

Y

V

N

D

K

A

H

H

P

P

E

E

F

Y

D

E

K

E

A

V

N

A

L

L

I

L

E

E

R

R

L

M

C

V

I

I

P

P

D

E

R

R

V

V

Y

I

Q

E

F

F

R

R

V

V

T

P

W

W

V

E

D

D

K

N

G

G

N

K

V

V

Q

H

T

V

N

N

M

T

G

G

Y

Y

R

R

V

V

Q

Q

H

F

N

N

N

G

A

A

I

I

G

G

P

P

Y

Y

K
|
K

G

G

I

L

R

R

F

F

H

A

A

P

S

S

V

V

N

N

L

L

S

S

I

I

L

M

K

K

F

F

L

L

A

G

F

F

E

E

Q

Q

T

A

F

F

K
|
K

N

D

S

S

L

L

T

T

L

L

P

P

M

M

G

G

G

A

K
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K

G

G

S

S

D

D

F

F

S

D

P

P

R

N

G

G

K

K

S

S

N

D

A

R

E

E

V

V

M

M

R

R

F

F

V

C

Q

Q

A

A

F

F

M

M

L

T

E

E

L

L

W

Y

R

R

H

H

I

I

G

G

P

P

E

D

T

I

D

D

V

V

P

P

A
|
A

G

G

D
|
D

I

L

G

G

V

V

G

G

G

A

R

R

E

E

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I

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M

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Y

G

G

M

Q

Y

Y

K

R

K

K

L

I

T

V

H

G

E

G

F

F

-

Y

T

N

G

G

T

V

F

L

T

T

G

G

K

K

G

A

R

R

E

S

F

F

G

G

S

S

L

L

I

V

R

R

P

P

E

E

A

A

T

T

G

G

Y

Y

G

G

N

S

I

V

Y

Y

F

Y

L

V

L

E

E

A

M

V

L

M

K

K

T

H

K

E

G

N

T

D

D

T

L

L

K

V

G

G

K

K

T

T

C

V

L

A

I

L

S

A

G

G

S

F

G

G

N

N

V

V

A

A

Q

W

Y

G

T

A

A

A

E

K

K

K

V

L

L

A

E

E

M

L

G

G

G

A

K

K

V

A

L

V

T

T

M

L

S

S

D

G

S

P

D

D

G

G

Y

Y

I

I

Y

Y

D

D

P

P

A

E

G

G

I

I

-

T

D

T

R

E

E

E

K

K

L

I

D

N

Y

Y

I

M

M

L

E

E

L

M

K

R

N

A

L

S

Y

G

R

R

G

N

R

K

I

V

R

Q

E

D

Y

Y

A

A

E

D

Q

K

Y

F

P

-

G

G

V

V

K

Q

Y

F

V

F

E

P

G

G

A

E

R

K

P

P

W

W

S

G

E

Q

K

K

A

V

D

D

I

I

A

I

L

M

P

P

S

C

A

A

T

T

Q

Q

N

N

E

D

I

V

N

D

G

L

D

E

D

Q

A

A

R

K

K

K

L

I

I

V

A

A

N

N

G

N

V

V

M

K

A

Y

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S

I

E

E

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A

A

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M

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T

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E

E

A

A

I

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K

R

-

F

V

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M

Q

Q

E

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A

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K

N

I

M

L

V

Y

V

A

A

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P

G

S

K

K

A

A

A

V

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N

A

A

G

G

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V

S

L

V

V

S
|
S

G

G

L

F

E

E

M

M

T

S

Q

Q

N

N

S

S

I

E

K

R

L

L

G

S

W

W

S

T

S

A

E

E

V

V

D

D

E

S

K

K

L

L

K

H

S

Q

I

V

M

M

K

T

N

D

I

I

H

H

-

D

-

G

-

S

-

A

A

A

C

A

V

E

Q

R

Y

Y

G

G

T

L

E

-

N

-

G

G

Y

Y

V

-

N

N

Y

L

V

V

K

A

G

G

A

A

N

N

V

I

A

V

G

G

F

F

M

Q

K

K

V

I

A

A

K

D

A

A

M

M

A

A

Q

Q

G

G

I

I

active site: K125 (= K124), D165 (= D164)
binding glutamic acid: K89 (= K88), K113 (= K112), A163 (= A162), S380 (= S377)

P24295 NAD-specific glutamate dehydrogenase; NAD-GDH; EC 1.4.1.2 from Clostridium symbiosum (Bacteroides symbiosus) (see 4 papers)
54% identity, 99% coverage: 5:444/445 of query aligns to 7:448/450 of P24295

query
sites
P24295

K

R

V

V

L

I

E

A

D

E

L

V

K

E

R

K

F

Y

P

A

N

D

E

E

P

P

E

E

Y

F

H

V

Q

Q

A

T

V

V

E

E

V

V

L

L

S

S

T

S

I

L

E

G

E

P

E

V

Y

V

N

D

K

A

H

H

P

P

E

E

F

Y

D

E

K

E

A

V

N

A

L

L

I

L

E

E

R

R

L

M

C

V

I

I

P

P

D

E

R

R

V

V

Y

I

Q

E

F

F

R

R

V

V

T

P

W

W

V

E

D

D

K

N

G

G

N

K

V

V

Q

H

T

V

N

N

M

T

G

G

Y

Y

R

R

V

V

Q

Q

H

F

N

N

N

G

A

A

I

I

G

G

P

P

Y

Y

K
|
K

G

G

I

L

R

R

F

F

H

A

A

P

S

S

V

V

N

N

L

L

S

S

I

I

L

M

K

K

F

F

L

L

A

G

F

F

E

E

Q
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Q

T

A

F

F

K
|
K

N

D

S

S

L

L

T

T

L

L

P

P

M

M

G

G

G

A

K
|
K

G

G

S

S

D

D

F

F

S

D

P

P

R

N

G

G

K

K

S

S

N

D

A

R

E

E

V

V

M

M

R

R

F

F

V

C

Q

Q

A

A

F

F

M

M

L

T

E

E

L

L

W

Y

R

R

H

H

I

I

G

G

P

P

E

D

T

I

D

D

V

V

P

P

A

A

G
|
G

D
|
D

I

L

G

G

V

V

G

G

G

A

R

R

E

E

V

I

G

G

F

Y

M

M

F

Y

G

G

M

Q

Y

Y

K

R

K

K

L

I

T

V

H

G

E

G

F

F

-

Y

T

N

G

G

T

V

F

L

T

T

G

G

K

K

G

A

R

R

E

S

F

F

G

G

S

S

L

L

I

V

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R

P

P

E

E

A

A

T

T

G

G

Y

Y

G

G

N

S

I

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Y

Y

F

Y

L

V

L

E

E

A

M

V

L

M

K

K

T

H

K

E

G

N

T

D

D

T

L

L

K

V

G

G

K

K

T

T

C

V

L

A

I

L

S

A

G

G

S

F

G

G

N

N

V

V

A

A

Q

W

Y

G

T

A

A

A

E

K

K

K

V

L

L

A

E

E

M

L

G

G

G

A

K

K

V

A

L

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M

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G

S

P

D

D

G

G

Y

Y

I

I

Y

Y

D

D

P

P

A

E

G

G

I

I

-

T

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E

E

E

K

K

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Y

Y

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M

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A

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Y

A

A

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D

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K

Y

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G

G

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K

Q

Y

F

V

F

E

P

G

G

A

E

R

K

P

P

W

W

S

G

E

Q

K

K

A

V

D

D

I

I

A

I

L

M

P

P

S

C

A

A

T

T

Q

Q

N

N

E

D

I

V

N

D

G

L

D

E

D

Q

A

A

R

K

K

K

L

I

I

V

A

A

N

N

G

N

V

V

M

K

A

Y

V

Y

S

I

E

E

G

V

A

A

N

N

M

M

P

P

S

T

T

T

P

N

E

E

A

A

I

L

K

R

-

F

V

L

F

M

Q

Q

E

Q

A

P

K

N

I

M

L

V

Y

V

A

A

P

P

G

S

K

K

A

A

A

V

N

N

A

A

G

G

V

V

S

L

V

V

S
|
S

G

G

L

F

E

E

M

M

T

S

Q

Q

N

N

S

S

I

E

K

R

L

L

G

S

W

W

S

T

S

A

E

E

V

V

D

D

E

S

K

K

L

L

K

H

S

Q

I

V

M

M

K

T

N

D

I

I

H

H

-

D

-

G

-

S

-

A

A

A

C

A

V

E

Q

R

Y

Y

G

G

T

L

E

-

N

-

G

G

Y

Y

V

-

N

N

Y

L

V

V

K

A

G

G

A

A

N

N

V

I

A

V

G

G

F

F

M

Q

K

K

V

I

A

A

K

D

A

A

M

M

A

A

Q

Q

G

G

I

I

K90 (= K88) binding substrate; mutation to L: Increased substrate activity for methionine and norleucine but negligible activity with either glutamate or leucine. Dramatic reduction in the dehydrogenase activity with glutamate as the substrate; when associated with V-381.
Q111 (= Q109) binding substrate
K114 (= K112) binding substrate
K126 (= K124) active site, Proton donor
G165 (= G163) binding substrate
D166 (= D164) mutation to S: Dramatic reduction in the dehydrogenase activity. Specific activity is decreased 1000-fold in the reductive amination reaction and 100000-fold for oxidative deamination.
S381 (= S377) binding substrate; mutation to V: Dramatic reduction in the dehydrogenase activity with glutamate as the substrate; when associated with L-90.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

5ijzA Crystal structure of glutamate dehydrogenase(gdh) from corynebacterium glutamicum (see paper)
55% identity, 98% coverage: 10:445/445 of query aligns to 14:447/447 of 5ijzA

query
sites
5ijzA

L

L

K

L

R

K

R

R

F

N

P

A

N

G

E

E

P

P

E

E

Y

F

H

H

Q

Q

A

A

V

V

E

A

E

E

V

V

L

L

S

E

T

S

I

L

E

K

E

I

E

V

Y

L

N

E

K

K

H

D

P

P

E

H

F

Y

D

A

K

D

A

Y

N

G

L

L

I

I

E

Q

R

R

L

L

C

C

I

E

P

P

D

E

R

R

V

Q

Y

L

Q

I

F

F

R

R

V

V

T

P

W

W

V

V

D

D

K

Q

G

G

N

Q

V

V

Q

H

T

V

N

N

M

R

G

G

Y

F

R

R

V

V

Q

Q

H

F

N

N

N

S

A

A

I

L

G

G

P

P

Y

Y

K
|
K

G
|
G

I

L

R

R

F

F

H

H

A

P

S

S

V

V

N

N

L

L

S

G

I

I

L

V

K

K

F

F

L

L

A

G

F

F

E

E

Q
|
Q

T

I

F

F

K
|
K

N

N

S

S

L

L

T

T

T

G

L

L

P

P

M

I

G

G

K
|
K

G

G

S

S

D

D

F

F

S

D

P

P

R
x
K

G

G

K

K

S

S

N

D

A

L

E

E

V

I

M

M

R

R

F

F

V

C

Q

Q

A

S

F

F

M

M

L

T

E

E

L

L

W

H

R

R

H

H

I

I

G

G

P

E

E

Y

T

R

D

D

V

V

P

P

A
|
A

G

G

D
|
D

I
|
I

G

G

V

V

G

G

R

R

E

E

V

I

G

G

F

Y

M

L

F

F

G

G

M

H

Y

Y

K

R

L

M

T

A

H

N

E

Q

F

H

-

E

T

S

G

G

T

V

F

L

T

T

G

G

K

K

G

G

R

L

E

T

F

W

G

G

S

S

L

L

I

V

R
|
R

P

T

E

E

A

A

T
|
T

G

G

Y

Y

G

G

N

C

I

V

Y

Y

F

F

L

V

L

S

E

E

M

M

L

I

K

K

T

A

K

K

G

G

T

E

D

S

L

I

K

S

G

G

K

Q

T

K

C

I

L

I

I

V

S

S

G

G

S
|
S

G
|
G

N
|
N

V
|
V

A

A

Q

T

Y

Y

T

A

A

I

E

E

K

K

V

A

L

Q

E

E

M

L

G

G

G

A

K

T

V

V

L

I

T

G

M

F

S

S

D
|
D

S
|
S

D

S

G

G

Y

W

I

V

Y

H

D

T

P

P

A

N

G

G

I

V

D

D

R

V

E

A

K

K

L

L

D

-

Y

-

I

-

M

R

E

E

L

I

K

K

N

E

L

V

Y

R

R

R

G

A

R
|
R

I

V

R

S

E

V

Y

Y

A

A

E

D

Q

E

Y

V

P

E

G

G

V

A

K

T

Y

Y

V

H

E

T

G

D

A

G

R

S

P

I

W

W

S

D

E

L

K

K

A

C

D

D

I

I

A

A

L

L

P

P

S

C

A
|
A

T
|
T

Q

Q

N

N

E

E

I

L

N

N

G

G

D

E

D

N

A

A

R

K

K

T

L

L

I

A

A

D

N

N

G

G

V

C

M

R

A

F

V

V

S

A

E

E

G

G

A
|
A

N
|
N

M

M

P

P

S

S

T

T

P

P

E

E

A

A

I

V

K

E

V

V

F

F

Q

R

E

E

A

R

K

D

I

I

L

R

Y

F

A

G

P

P

G

G

K

K

A

A

N
|
N

A

A

G

G

V
|
V

S

A

V

T

S
|
S

G

A

L

L

E

E

M

M

T

Q

Q

Q

N

N

S

A

I

S

K

R

L

D

G

S

W

W

S

S

S

F

E

E

E

Y

V

T

D

D

E

E

K

R

L

L

K

Q

S

V

I

I

M

M

K

K

N

N

I

I

H

F

A

K

A

T

C

C

V

A

Q

E

Y

T

G

A

T

A

E

E

E

Y

N

G

G

H

Y

E

V

N

N

D

Y

Y

V

V

K

V

G

G

A

A

N

N

V

I

A

A

G

G

F

F

M

K

K

K

V

V

A

A

K

D

A

A

M

M

M

L

A

A

Q

Q

G

G

I

V

L

I

active site: K128 (= K124), D168 (= D164)
binding 2-oxoglutaric acid: K92 (= K88), G93 (= G89), G94 (= G90), Q113 (= Q109), K116 (= K112), K128 (= K124), A166 (= A162), R208 (= R203), V376 (= V374), S379 (= S377)
binding nadp nicotinamide-adenine-dinucleotide phosphate: K136 (≠ R132), D168 (= D164), I169 (= I165), T212 (= T207), S241 (= S236), G242 (= G237), N243 (= N238), V244 (= V239), D264 (= D259), S265 (= S260), R290 (= R288), A321 (= A319), T322 (= T320), A346 (= A344), N347 (= N345), N372 (= N370)

5gudA Glutamate dehydrogenase from corynebacterium glutamicum (alpha- iminoglutarate/NADP+ complex) (see paper)
55% identity, 98% coverage: 10:445/445 of query aligns to 14:447/447 of 5gudA

query
sites
5gudA

L

L

K

L

R

K

R

R

F

N

P

A

N

G

E

E

P

P

E

E

Y

F

H

H

Q

Q

A

A

V

V

E

A

E

E

V

V

L

L

S

E

T

S

I

L

E

K

E

I

E

V

Y

L

N

E

K

K

H

D

P

P

E

H

F

Y

D

A

K

D

A

Y

N

G

L

L

I

I

E

Q

R

R

L

L

C

C

I

E

P

P

D

E

R

R

V

Q

Y

L

Q

I

F

F

R

R

V

V

T

P

W

W

V

V

D

D

K

Q

G

G

N

Q

V

V

Q

H

T

V

N

N

M

R

G

G

Y

F

R

R

V

V

Q

Q

H

F

N

N

N

S

A

A

I

L

G

G

P

P

Y

Y

K
|
K

G
|
G

I

L

R

R

F

F

H

H

A

P

S

S

V

V

N

N

L

L

S

G

I

I

L

V

K

K

F

F

L

L

A

G

F

F

E

E

Q
|
Q

T

I

F

F

K
|
K

N

N

S

S

L

L

T

T

T

G

L

L

P

P

M

I

G

G

K
|
K

G

G

S

S

D

D

F

F

S

D

P

P

R
x
K

G

G

K

K

S

S

N

D

A

L

E

E

V

I

M

M

R

R

F

F

V

C

Q

Q

A

S

F

F

M

M

L

T

E

E

L

L

W

H

R

R

H

H

I

I

G

G

P

E

E

Y

T

R

D

D

V

V

P

P

A
|
A

G

G

D
|
D

I
|
I

G

G

V

V

G

G

R

R

E

E

V

I

G

G

F

Y

M

L

F

F

G

G

M

H

Y

Y

K

R

L

M

T

A

H

N

E

Q

F

H

-

E

T

S

G

G

T

V

F

L

T

T

G

G

K

K

G

G

R

L

E

T

F

W

G

G

S

S

L

L

I

V

R
|
R

P

T

E

E

A

A

T
|
T

G

G

Y

Y

G

G

N

C

I

V

Y

Y

F

F

L

V

L

S

E

E

M

M

L

I

K

K

T

A

K

K

G

G

T

E

D

S

L

I

K

S

G

G

K

Q

T

K

C

I

L

I

I

V

S

S

G

G

S
|
S

G
|
G

N
|
N

V
|
V

A

A

Q

T

Y

Y

T

A

A

I

E

E

K

K

V

A

L

Q

E

E

M

L

G

G

G

A

K

T

V

V

L

I

T

G

M

F

S

S

D
|
D

S
|
S

D

S

G

G

Y

W

I

V

Y

H

D

T

P

P

A

N

G

G

I

V

D

D

R

V

E

A

K

K

L

L

D

-

Y

-

I

-

M

R

E

E

L

I

K

K

N

E

L

V

Y

R

R

R

G

A

R
|
R

I

V

R

S

E

V

Y

Y

A

A

E

D

Q

E

Y

I

P

E

G

G

V

A

K

T

Y

Y

V

H

E

T

G

D

A

G

R

S

P

I

W

W

S

D

E

L

K

K

A

C

D

D

I

I

A

A

L

L

P

P

S

C

A
|
A

T
|
T

Q

Q

N

N

E

E

I

L

N

N

G

G

D

E

D

N

A

A

R

K

K

T

L

L

I

A

A

D

N

N

G

G

V

C

M

R

A

F

V

V

S

A

E

E

G
|
G

A
|
A

N
|
N

M

M

P

P

S

S

T

T

P

P

E

E

A

A

I

V

K

E

V

V

F

F

Q

R

E

E

A

R

K

D

I

I

L

R

Y

F

A

G

P

P

G

G

K

K

A

A

N
|
N

A

A

G

G

V
|
V

S

A

V

T

S
|
S

G

A

L

L

E

E

M

M

T

Q

Q

Q

N

N

S

A

I

S

K

R

L

D

G

S

W

W

S

S

S

F

E

E

E

Y

V

T

D

D

E

E

K

R

L

L

K

Q

S

V

I

I

M

M

K

K

N

N

I

I

H

F

A

K

A

T

C

C

V

A

Q

E

Y

T

G

A

T

A

E

E

E

Y

N

G

G

H

Y

E

V

N

N

D

Y

Y

V

V

K

V

G

G

A

A

N

N

V

I

A

A

G

G

F

F

M

K

K

K

V

V

A

A

K

D

A

A

M

M

M

L

A

A

Q

Q

G

G

I

V

L

I

active site: K128 (= K124), D168 (= D164)
binding (2Z)-2-iminopentanedioic acid: K92 (= K88), G93 (= G89), G94 (= G90), Q113 (= Q109), K116 (= K112), K128 (= K124), A166 (= A162), R208 (= R203), V376 (= V374), S379 (= S377)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: K136 (≠ R132), D168 (= D164), I169 (= I165), R208 (= R203), T212 (= T207), S241 (= S236), G242 (= G237), N243 (= N238), V244 (= V239), D264 (= D259), S265 (= S260), R290 (= R288), A321 (= A319), T322 (= T320), G345 (= G343), A346 (= A344), N347 (= N345), N372 (= N370)

5gudE Glutamate dehydrogenase from corynebacterium glutamicum (alpha- iminoglutarate/NADP+ complex) (see paper)
55% identity, 98% coverage: 10:445/445 of query aligns to 27:460/460 of 5gudE

query
sites
5gudE

L

L

K

L

R

K

R

R

F

N

P

A

N

G

E

E

P

P

E

E

Y

F

H

H

Q

Q

A

A

V

V

E

A

E

E

V

V

L

L

S

E

T

S

I

L

E

K

E

I

E

V

Y

L

N

E

K

K

H

D

P

P

E

H

F

Y

D

A

K

D

A

Y

N

G

L

L

I

I

E

Q

R

R

L

L

C

C

I

E

P

P

D

E

R

R

V

Q

Y

L

Q

I

F

F

R

R

V

V

T

P

W

W

V

V

D

D

K

Q

G

G

N

Q

V

V

Q

H

T

V

N

N

M

R

G

G

Y

F

R

R

V

V

Q

Q

H

F

N

N

N

S

A

A

I

L

G

G

P

P

Y

Y

K

K

G

G

I

L

R

R

F

F

H

H

A

P

S

S

V

V

N

N

L

L

S

G

I

I

L

V

K

K

F

F

L

L

A

G

F

F

E

E

Q

Q

T

I

F

F

K

K

N

N

S

S

L

L

T

T

T

G

L

L

P

P

M

I

G

G

K
|
K

G

G

S

S

D

D

F

F

S

D

P

P

R

K

G

G

K

K

S

S

N

D

A

L

E

E

V

I

M

M

R

R

F

F

V

C

Q

Q

A

S

F

F

M

M

L

T

E

E

L

L

W

H

R

R

H

H

I

I

G

G

P

E

E

Y

T

R

D

D

V

V

P

P

A

A

G

G

D
|
D

I

I

G

G

V

V

G

G

R

R

E

E

V

I

G

G

F

Y

M

L

F

F

G

G

M

H

Y

Y

K

R

L

M

T

A

H

N

E

Q

F

H

-

E

T

S

G

G

T

V

F

L

T

T

G

G

K

K

G

G

R

L

E

T

F

W

G

G

S

S

L

L

I

V

R

R

P

T

E

E

A

A

T
|
T

G

G

Y

Y

G

G

N

C

I

V

Y

Y

F

F

L

V

L

S

E

E

M

M

L

I

K

K

T

A

K

K

G

G

T

E

D

S

L

I

K

S

G

G

K

Q

T

K

C

I

L

I

I

V

S

S

G

G

S
|
S

G
|
G

N
|
N

V
|
V

A

A

Q

T

Y

Y

T

A

A

I

E

E

K

K

V

A

L

Q

E

E

M

L

G

G

G

A

K

T

V

V

L

I

T

G

M

F

S

S

D
|
D

S
|
S

D

S

G

G

Y

W

I

V

Y

H

D

T

P

P

A

N

G

G

I

V

D

D

R

V

E

A

K

K

L

L

D

-

Y

-

I

-

M

R

E

E

L

I

K

K

N

E

L

V

Y

R

R

R

G

A

R
|
R

I

V

R

S

E

V

Y

Y

A

A

E

D

Q

E

Y

I

P

E

G

G

V

A

K

T

Y

Y

V

H

E

T

G

D

A

G

R

S

P

I

W

W

S

D

E

L

K

K

A

C

D

D

I

I

A

A

L

L

P

P

S

C

A
|
A

T
|
T

Q

Q

N

N

E

E

I

L

N

N

G

G

D

E

D

N

A

A

R

K

K

T

L

L

I

A

A

D

N

N

G

G

V

C

M

R

A

F

V

V

S

A

E

E

G

G

A
|
A

N
|
N

M

M

P

P

S

S

T

T

P

P

E

E

A

A

I

V

K

E

V

V

F

F

Q

R

E

E

A

R

K

D

I

I

L

R

Y

F

A

G

P

P

G

G

K

K

A

A

N

N

A

A

G

G

V

V

S

A

V

T

S

S

G

A

L

L

E

E

M

M

T

Q

Q

Q

N

N

S

A

I

S

K

R

L

D

G

S

W

W

S

S

S

F

E

E

E

Y

V

T

D

D

E

E

K

R

L

L

K

Q

S

V

I

I

M

M

K

K

N

N

I

I

H

F

A

K

A

T

C

C

V

A

Q

E

Y

T

G

A

T

A

E

E

E

Y

N

G

G

H

Y

E

V

N

N

D

Y

Y

V

V

K

V

G

G

A

A

N

N

V

I

A

A

G

G

F

F

M

K

K

K

V

V

A

A

K

D

A

A

M

M

M

L

A

A

Q

Q

G

G

I

V

L

I

active site: K141 (= K124), D181 (= D164)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: T225 (= T207), S254 (= S236), G255 (= G237), N256 (= N238), V257 (= V239), D277 (= D259), S278 (= S260), R303 (= R288), A334 (= A319), T335 (= T320), A359 (= A344), N360 (= N345)

7f79C Crystal structure of glutamate dehydrogenase 3 from candida albicans in complex with alpha-ketoglutarate and NADPH (see paper)
52% identity, 97% coverage: 14:443/445 of query aligns to 5:455/458 of 7f79C

query
sites
7f79C

F

L

P

P

N

H

E

E

P

P

E

E

Y

F

H

Q

Q

Q

A

A

V

Y

E

D

E

E

V

L

L

V

S

S

T

A

I

V

E

E

E

D

E

S

-

T

-

L

Y

F

N

K

K

E

H

E

P

P

E

Q

F

Y

D

K

K

K

A

-

N

-

L

V

I

I

E

P

R

V

L

V

C

S

I

I

P

P

D

E

R

R

V

I

Y

I

Q

Q

F

F

R

R

V

V

T

T

W

W

V

E

D

N

D

D

K

K

G

G

N

E

V

I

Q

E

T

V

N

N

M

N

G

G

Y

F

R

R

V

V

Q

Q

H

Y

N

N

N

S

A

A

I

L

G

G

P

P

Y

Y

K
|
K

G
|
G

I

L

R
|
R

F

F

H
|
H

A

P

S

T

V

V

N

N

L

L

S

S

I

I

L

L

K

K

F

F

L

L

A

G

F

F

E

E

Q
|
Q

T

I

F

F

K
|
K

N

N

S

A

L

L

T

T

T

G

L

L

P

S

M

M

G

G

K
|
K

G

G

S

S

D

D

F

F

S

N

P

P

R
x
K

G

N

K

R

S

S

N

D

A

N

E

E

V

I

M

R

R

R

F

F

V

C

Q

V

A

S

F

F

M

M

L

R

E

Q

L

L

W

A

R

R

H

Y

I

I

G

G

P

P

E

D

T

T

D

D

V

V

P

P

A

A

G

G

D
|
D

I
|
I

G

G

V

V

G

G

R

R

E

E

V

V

G

G

F

F

M

L

F

F

G

G

M

A

Y

Y

K

K

K

Q

L

M

T

R

H

N

E

N

F

W

T

A

G

G

T

V

F

L

T

T

G

G

K

K

G

G

R

L

E

T

F

W

G

G

S

S

L

L

I

I

R
|
R

P

P

E

E

A

A

T
|
T

G

G

Y

Y

G

G

N

C

I

V

Y

Y

F

Y

L

V

L

E

E

K

M

M

L

I

K

E

-

K

-

A

T

T

K

N

G

G

T

K

D

E

-

T

L

F

K

K

G

G

K

K

T

R

C

V

L

A

I

I

S

S

G

G

S
|
S

G
|
G

N
|
N

V
|
V

A

A

Q

Q

Y

Y

T

A

A

A

E

L

K

K

V

V

L

I

E

E

M

L

G

G

K

T

V

V

L

V

T

S

M

L

S

S

D
|
D

S
|
S

D

K

G

G

Y

S

I

I

Y

I

D

S

P

K

A

N

G

G

I

I

D

T

R

A

E

D

K

Q

L

V

D

Y

Y

A

I

I

M

A

E

A

L

A

K
|
K

N

L

L

K

Y

F

R

K

G

S

R

L

-

E

-

I

I

V

R

A

E

D

Y

S

A

V

E

Q

Q

L

Y

F

P

S

G

G

-

D

-

H

-

S

V

V

K

E

Y

Y

V

L

E

A

G

G

A

V

R

R

P

P

W

W

S

T

E

K

-

V

-

G

K

Q

A

V

D

D

I

V

A

A

L

L

P

P

S

S

A
|
A

T
|
T

Q

Q

N

N

E

E

I

V

N

S

G

G

D

E

A

A

R

K

K

A

L

L

I

V

A

D

N

A

G

G

V

C

M

K

A

F

V

I

S

A

E

E

G
|
G

A
x
S

N
|
N

M

M

P

G

S

S

T

T

P

K

E

E

A

A

I

I

K

E

V

V

F

F

Q

E

E

A

A

N

K

R

-

D

-

S

-

N

-

G

I

V

L

W

Y

Y

A

A

P

P

G

G

K

K

A

A

N
|
N

A

C

G

G

V
|
V

S

A

V

V

S
|
S

G

G

L

L

E

E

M

M

T

A

Q

Q

N

N

S

S

I

Q

K

R

L

V

G

Q

W

W

S

T

S

N

E

E

V

V

D

D

E

A

K

K

L

L

K

K

S

E

I

I

M

M

-

Y

-

T

-

C

-

F

K

E

N

N

I

C

H

Y

A

K

A

T

C

A

V

Q

Q

K

Y

Y

G

S

T

I

E

E

-

K

-

N

E

E

N

N

G

G

Y

L

V

P

N

S

Y

L

V

L

K

K

G

G

A

A

N

N

V

I

A

A

G

G

F

F

M

I

K

K

V

V

A

A

K

D

A

A

M

M

M

F

A

D

Q

Q

G

G

binding 2-oxoglutaric acid: K79 (= K88), G80 (= G89), G81 (= G90), Q100 (= Q109), K103 (= K112), K115 (= K124), V380 (= V374), S383 (= S377)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: R83 (= R92), H85 (= H94), K123 (≠ R132), D155 (= D164), I156 (= I165), R194 (= R203), T198 (= T207), S230 (= S236), G231 (= G237), N232 (= N238), V233 (= V239), D253 (= D259), S254 (= S260), K276 (= K282), A321 (= A319), T322 (= T320), G345 (= G343), S346 (≠ A344), N347 (= N345), N376 (= N370)

P00369 NADP-specific glutamate dehydrogenase; NADP-GDH; NADP-dependent glutamate dehydrogenase; EC 1.4.1.4 from Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) (see paper)
54% identity, 97% coverage: 14:443/445 of query aligns to 4:448/454 of P00369

query
sites
P00369

F

L

P

P

N

S

E

E

P

P

E

E

Y

F

H

E

Q

Q

A

A

V

Y

E

K

E

E

V

L

L

A

S

Y

T

T

I

L

E

E

E

N

E

S

-

S

-

L

Y

F

N

Q

K

K

H

H

P

P

E

E

F

Y

D

R

K

T

A

A

N

L

L

T

I

V

E

A

R

S

L

-

C

-

I

I

P

P

D

E

R

R

V

V

Y

I

Q

Q

F

F

R

R

V

V

T

V

W

W

V

E

D

D

K

N

G

G

N

N

V

V

Q

Q

T

V

N

N

M

R

G

G

Y

Y

R

R

V

V

Q

Q

H

F

N

N

N

S

A

A

I

L

G

G

P

P

Y

Y

K

K

G

G

I

L

R

R

F

L

H

H

A

P

S

S

V

V

N

N

L

L

S

S

I

I

L

L

K

K

F

F

L

L

A

G

F

F

E

E

Q

Q

T

I

F

F

K

K

N

N

S

A

L

L

T

T

T

G

L

L

P

S

M

M

G

G

K

K

G

G

S

A

D

D

F

F

S

D

P

P

R

K

G

G

K

K

S

S

N

D

A

A

E

E

V

I

M

R

R

R

F

F

V

C

Q

C

A

A

F

F

M

M

L

A

E

E

L

L

W

H

R

K

H

H

I

I

G

G

P

A

E

D

T

T

D

D

V

V

P

P

A

A

G

G

D

D

I

I

G

G

V

V

G

G

R

R

E

E

V

I

G

G

F

Y

M

M

F

F

G

G

M

A

Y

Y

K

R

K

K

L

A

T

A

H

N

E

R

F

F

T

E

G

G

T

V

F

L

T

T

G

G

K

K

G

G

R

L

E

S

F

W

G

G

S

S

L

L

I

I

R

R

P

P

E

E

A

A

T

T

G

G

Y

Y

G

G

N

L

I

V

Y

Y

F

Y

L

V

L

G

E

H

M

M

L

L

K

E

T

Y

K

S

G

G

T

A

-

G

D

S

L

Y

K

A

G

G

K

K

T

R

C

V

L

A

I

L

S

S

G

G

S

S

G

G

N

N

V

V

A

A

Q

Q

Y

Y

T

A

A

A

E

L

K

K

V

L

L

I

E

E

M

L

G

G

G

A

K

T

V

V

L

V

T

S

M

L

S

S

D

D

S

S

D

K

G

G

Y

A

I

L

Y

V

D

A

P

T

-

G

-

E

A

S

G

G

I

I

D

T

R

V

E

E

K

D

L

I

D

N

Y

A

I

V

M

M

E

A

L

I

K

K

N

-

L

-

Y

-

R

E

G

A

R

R

I

Q

R

S

E

L

Y

T

A

S

E

F

Q

Q

Y

H

P

A

G

G

-

H

V

L

K

K

Y

W

V

I

E

E

G

G

A

A

R

R

P

P

W

W

S

L

E

H

-

V

-

G

K

K

A

V

D

D

I

I

A

A

L

L

P

P

S

C

A

A

T

T

Q

Q

N

N

E

E

I

V

N

S

G

K

D

E

A

A

R

E

K

G

L

L

I

L

A

A

N

A

G

G

V

C

M

K

A

F

V

V

S

A

E

E

G

G

A

S

N

N

M

M

P

G

S

C

T

T

P

L

E

E

A

A

I

I

K

E

V

V

F

F

Q

E

E

N

A

N

K

R

-

K

-

E

-

K

-

G

-

E

-

A

I

V

L

W

Y

Y

A

A

P

P

G

G

K

K

A

A

N

N

A

C

G

G

V

V

S

A

V

V

S

S

G

G

L

L

E

E

M

M

T

A

Q

Q

N

N

S

S

I

Q

K

R

L

L

G

N

W

W

S

T

S

Q

E

A

E

E

V

V

D

D

E

E

K

K

L

L

K

K

S

D

I

I

M

M

K

K

N

N

-

A

-

F

-

N

-

G

I

L

H

N

A

T

A

A

C

K

V

T

Q

Y

Y

V

G

E

T

A

E

A

E

E

N

G

G

E

Y

L

V

P

N

S

Y

L

V

V

K

A

G

G

A

S

N

N

V

I

A

A

G

G

F

F

M

V

K

K

V

V

A

A

K

Q

A

A

M

M

M

H

A

D

Q

Q

G

G

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylserine

5xwcA Crystal structure of aspergillus niger glutamate dehydrogenase complexed with alpha-iminoglutarate, 2-amino-2-hydroxyglutarate and NADP (see paper)
54% identity, 97% coverage: 14:443/445 of query aligns to 3:456/459 of 5xwcA

query
sites
5xwcA

F

L

P

P

N

H

E

E

P

P

E

E

Y

F

H

E

Q

Q

A

A

V

Y

E

K

E

E

V

L

L

A

S

S

T

T

I

L

E

E

E

N

E

S

-

T

-

L

Y

F

N

Q

K

K

H

N

P

P

E

E

F

Y

D

R

K

K

A

A

N

-

L

-

I

L

E

A

R

V

L

V

C

S

I

V

P

P

D

E

R

R

V

V

Y

I

Q

Q

F

F

R

R

V

V

T

V

W

W

V

E

D

D

K

A

G

G

N

N

V

V

Q

Q

T

V

N

N

M

R

G

G

Y

F

R

R

V

V

Q

Q

H

F

N

N

N

S

A

A

I

L

G

G

P

P

Y

Y

K
|
K

G
|
G

I

L

R

R

F

F

H
|
H

A

P

S

S

V

V

N

N

L

L

S

S

I

I

L

L

K

K

F

F

L

L

A

G

F

F

E

E

Q
|
Q

T

I

F

F

K
|
K

N

N

S

A

L

L

T

T

T

G

L

L

P

N

M

M

G

G

K
|
K

G

G

S

S

D

D

F

F

S

D

P

P

R
x
K

G

G

K

K

S

S

N

D

A

N

E

E

V

I

M

R

R

R

F

F

V

C

Q

V

A

S

F

F

M

M

L

T

E

E

L

L

W

C

R

K

H

H

I

I

G

G

P

A

E

D

T

T

D

D

V

V

P

P

A
|
A

G

G

D
|
D

I
|
I

G

G

V

V

G

T

G

G

R

R

E

E

V

V

G

G

F

F

M

L

F

F

G

G

M

Q

Y

Y

K

R

K

K

L

I

T

R

H

N

E

Q

F

W

T

E

G

G

T

V

F

L

T

T

G

G

K

K

G

G

R

G

E

S

F

W

G

G

S

S

L

L

I

I

R
|
R

P

P

E

E

A

A

T
|
T

G

G

Y

Y

G

G

N

V

I

V

Y

Y

F

Y

L

V

L

E

E

H

M

M

L

I

-

A

-

H

K

A

T

T

K

N

G

G

T

Q

D

E

-

S

L

F

K

K

G

G

K

K

T

R

C

V

L

A

I

I

S

S

G

G

S
|
S

G
|
G

N
|
N

V
|
V

A

A

Q

Q

Y

Y

T

A

A

A

E

L

K

K

V

V

L

I

E

E

M

L

G

G

K

S

V

V

L

V

T

S

M

L

S

S

D
|
D

S
|
S

D

Q

G

G

-

S

-

L

Y

I

I

I

Y

N

D

G

P

E

A

G

G

S

I

F

D

T

R

P

E

E

K

E

L

I

D

E

Y

L

I

I

M

A

E

Q

L

T

K

K

N

-

L

V

Y

E

R

R

G

K

R

Q

I

L

R

A

E

S

Y

I

A

V

E

G

Q

A

Y

A

P

P

-

F

-

S

-

D

-

A

-

N

G

K

V

F

K

K

Y

Y

V

I

E

A

G

G

A

A

R

R

P

P

W

W

S

V

E

H

-

V

-

G

K

K

A

V

D

D

I

V

A

A

L

L

P

P

S

S

A
|
A

T
|
T

Q

Q

N

N

E

E

I

I

N

S

G

G

D

E

A

A

R

Q

K

V

L

L

I

I

A

N

N

A

G

G

V

C

M

K

A

F

V

I

S

A

E

E

G
|
G

A
x
S

N
|
N

M

M

P

G

S

C

T

T

P

Q

E

E

A

A

I

I

K

D

V

T

F

F

Q

E

-

A

-

H

-

R

-

T

-

A

-

N

-

A

-

G

E

A

A

A

K

A

I

I

L

W

Y

Y

A

A

P

P

G

G

K

K

A

A

N
|
N

A

A

G

G

V
|
V

S

A

V

V

S
|
S

G

G

L

L

E

E

M

M

T

A

Q

Q

N

N

S

S

I

A

K

R

L

L

G

S

W

W

S

T

S

S

E

E

V

V

D

D

E

A

K

R

L

L

K

K

S

D

I

I

M

M

-

R

-

D

-

C

-

F

K

K

N

N

I

G

H

L

A

E

A

T

C

A

V

Q

Q

E

Y

Y

G

A

T

T

E

P

E

A

N

E

G

G

Y

V

V

L

-

P

N

S

Y

L

V

V

K

T

G

G

A

S

N

N

V

I

A

A

G

G

F

F

M

T

K

K

V

V

A

A

K

A

A

A

M

M

M

K

A

D

Q

Q

G

G

binding (2Z)-2-iminopentanedioic acid: K77 (= K88), G78 (= G89), Q98 (= Q109), K101 (= K112), K113 (= K124), A151 (= A162), R192 (= R203), V382 (= V374), S385 (= S377)
binding (2S)-2-azanyl-2-oxidanyl-pentanedioic acid: K77 (= K88), G78 (= G89), G79 (= G90), Q98 (= Q109), K101 (= K112), K113 (= K124), A151 (= A162), D153 (= D164), R192 (= R203), V382 (= V374), S385 (= S377)
binding nadp nicotinamide-adenine-dinucleotide phosphate: H83 (= H94), K121 (≠ R132), D153 (= D164), I154 (= I165), R192 (= R203), T196 (= T207), S228 (= S236), G229 (= G237), N230 (= N238), V231 (= V239), D251 (= D259), S252 (= S260), A319 (= A319), T320 (= T320), G343 (= G343), S344 (≠ A344), N345 (= N345), N378 (= N370)

5xw0A Crystal structure of aspergillus niger glutamate dehydrogenase complexed with isophthalate and NADPH (see paper)
54% identity, 97% coverage: 14:443/445 of query aligns to 3:456/459 of 5xw0A

query
sites
5xw0A

F

L

P

P

N

H

E

E

P

P

E

E

Y

F

H

E

Q

Q

A

A

V

Y

E

K

E

E

V

L

L

A

S

S

T

T

I

L

E

E

E

N

E

S

-

T

-

L

Y

F

N

Q

K

K

H

N

P

P

E

E

F

Y

D

R

K

K

A

A

N

-

L

-

I

L

E

A

R

V

L

V

C

S

I

V

P

P

D

E

R

R

V

V

Y

I

Q

Q

F

F

R

R

V

V

T

V

W

W

V

E

D

D

K

A

G

G

N

N

V

V

Q

Q

T

V

N

N

M

R

G

G

Y

F

R

R

V

V

Q

Q

H

F

N

N

N

S

A

A

I

L

G

G

P

P

Y

Y

K
|
K

G
|
G

G

G

I

L

R

R

F

F

H
|
H

A

P

S

S

V

V

N

N

L

L

S

S

I

I

L

L

K

K

F

F

L

L

A

G

F

F

E

E

Q
|
Q

T

I

F

F

K
|
K

N

N

S

A

L

L

T

T

T

G

L

L

P

N

M

M

G

G

K
|
K

G

G

S

S

D

D

F

F

S

D

P

P

R
x
K

G

G

K

K

S

S

N

D

A

N

E

E

V

I

M

R

R

R

F

F

V

C

Q

V

A

S

F

F

M

M

L

T

E

E

L

L

W

C

R

K

H

H

I

I

G

G

P

A

E

D

T

T

D

D

V

V

P

P

A
|
A

G
|
G

D
|
D

I
|
I

G

G

V

V

G

T

G

G

R

R

E

E

V

V

G

G

F

F

M

L

F

F

G

G

M

Q

Y

Y

K

R

K

K

L

I

T

R

H

N

E

Q

F

W

T

E

G

G

T

V

F

L

T

T

G

G

K

K

G

G

R

G

E

S

F

W

G

G

S

S

L

L

I

I

R
|
R

P

P

E

E

A

A

T
|
T

G

G

Y

Y

G

G

N

V

I

V

Y

Y

F

Y

L

V

L

E

E

H

M

M

L

I

-

A

-

H

K

A

T

T

K

N

G

G

T

Q

D

E

-

S

L

F

K

K

G

G

K

K

T

R

C

V

L

A

I

I

S

S

G

G

S
|
S

G
|
G

N
|
N

V
|
V

A

A

Q

Q

Y

Y

T

A

A

A

E

L

K

K

V

V

L

I

E

E

M

L

G

G

K

S

V

V

L

V

T

S

M

L

S

S

D
|
D

S
|
S

D

Q

G

G

-

S

-

L

Y

I

I

I

Y

N

D

G

P

E

A

G

G

S

I

F

D

T

R

P

E

E

K

E

L

I

D

E

Y

L

I

I

M

A

E

Q

L

T

K

K

N

-

L

V

Y

E

R

R

G

K

R

Q

I

L

R

A

E

S

Y

I

A

V

E

G

Q

A

Y

A

P

P

-

F

-

S

-

D

-

A

-

N

G

K

V

F

K

K

Y

Y

V

I

E

A

G

G

A

A

R

R

P

P

W

W

S

V

E

H

-

V

-

G

K

K

A

V

D

D

I

V

A

A

L

L

P

P

S

S

A
|
A

T
|
T

Q

Q

N

N

E

E

I

I

N

S

G

G

D

E

A

A

R

Q

K

V

L

L

I

I

A

N

N

A

G

G

V

C

M

K

A

F

V

I

S

A

E

E

G
|
G

A
x
S

N
|
N

M

M

P

G

S

C

T

T

P

Q

E

E

A

A

I

I

K

D

V

T

F

F

Q

E

-

A

-

H

-

R

-

T

-

A

-

N

-

A

-

G

E

A

A

A

K

A

I

I

L

W

Y

Y

A

A

P

P

G

G

K

K

A

A

N
|
N

A

A

G

G

V

V

S

A

V

V

S
|
S

G

G

L

L

E

E

M

M

T

A

Q

Q

N

N

S

S

I

A

K

R

L

L

G

S

W

W

S

T

S

S

E

E

V

V

D

D

E

A

K

R

L

L

K

K

S

D

I

I

M

M

-

R

-

D

-

C

-

F

K

K

N

N

I

G

H

L

A

E

A

T

C

A

V

Q

Q

E

Y

Y

G

A

T

T

E

P

E

A

N

E

G

G

Y

V

V

L

-

P

N

S

Y

L

V

V

K

T

G

G

A

S

N

N

V

I

A

A

G

G

F

F

M

T

K

K

V

V

A

A

K

A

A

A

M

M

M

K

A

D

Q

Q

G

G

binding benzene-1,3-dicarboxylic acid: K77 (= K88), G78 (= G89), Q98 (= Q109), K101 (= K112), K113 (= K124), A151 (= A162), G152 (= G163), D153 (= D164), R192 (= R203), S385 (= S377)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: H83 (= H94), K121 (≠ R132), D153 (= D164), I154 (= I165), R192 (= R203), T196 (= T207), S228 (= S236), G229 (= G237), N230 (= N238), V231 (= V239), D251 (= D259), S252 (= S260), A319 (= A319), T320 (= T320), G343 (= G343), S344 (≠ A344), N345 (= N345), N378 (= N370)

5xvxA Crystal structure of aspergillus niger glutamate dehydrogenase complexed with alpha-ketoglutarate and NADPH (see paper)
54% identity, 97% coverage: 14:443/445 of query aligns to 3:456/459 of 5xvxA

query
sites
5xvxA

F

L

P

P

N

H

E

E

P

P

E

E

Y

F

H

E

Q

Q

A

A

V

Y

E

K

E

E

V

L

L

A

S

S

T

T

I

L

E

E

E

N

E

S

-

T

-

L

Y

F

N

Q

K

K

H

N

P

P

E

E

F

Y

D

R

K

K

A

A

N

-

L

-

I

L

E

A

R

V

L

V

C

S

I

V

P

P

D

E

R

R

V

V

Y

I

Q

Q

F

F

R

R

V

V

T

V

W

W

V

E

D

D

K

A

G

G

N

N

V

V

Q

Q

T

V

N

N

M

R

G

G

Y

F

R

R

V

V

Q

Q

H

F

N

N

N

S

A

A

I

L

G

G

P

P

Y

Y

K
|
K

G

G

I

L

R

R

F

F

H

H

A

P

S

S

V

V

N

N

L

L

S

S

I

I

L

L

K

K

F

F

L

L

A

G

F

F

E

E

Q
|
Q

T

I

F

F

K
|
K

N

N

S

A

L

L

T

T

T

G

L

L

P

N

M

M

G

G

K
|
K

G

G

S

S

D

D

F

F

S

D

P

P

R
x
K

G

G

K

K

S

S

N

D

A

N

E

E

V

I

M

R

R

R

F

F

V

C

Q

V

A

S

F

F

M

M

L

T

E

E

L

L

W

C

R

K

H

H

I

I

G

G

P

A

E

D

T

T

D

D

V

V

P

P

A
|
A

G

G

D
|
D

I
|
I

G

G

V

V

G

T

G

G

R

R

E

E

V

V

G

G

F

F

M

L

F

F

G

G

M

Q

Y

Y

K

R

K

K

L

I

T

R

H

N

E

Q

F

W

T

E

G

G

T

V

F

L

T

T

G

G

K

K

G

G

R

G

E

S

F

W

G

G

S

S

L

L

I

I

R
|
R

P

P

E

E

A

A

T
|
T

G

G

Y

Y

G

G

N

V

I

V

Y

Y

F

Y

L

V

L

E

E

H

M

M

L

I

-

A

-

H

K

A

T

T

K

N

G

G

T

Q

D

E

-

S

L

F

K

K

G

G

K

K

T

R

C

V

L

A

I

I

S

S

G

G

S
|
S

G
|
G

N
|
N

V
|
V

A

A

Q

Q

Y

Y

T

A

A

A

E

L

K

K

V

V

L

I

E

E

M

L

G

G

K

S

V

V

L

V

T

S

M

L

S

S

D
|
D

S
|
S

D

Q

G

G

-

S

-

L

Y

I

I

I

Y

N

D

G

P

E

A

G

G

S

I

F

D

T

R

P

E

E

K

E

L

I

D

E

Y

L

I

I

M

A

E

Q

L

T

K

K

N

-

L

V

Y

E

R

R

G

K

R

Q

I

L

R

A

E

S

Y

I

A

V

E

G

Q

A

Y

A

P

P

-

F

-

S

-

D

-

A

-

N

G

K

V

F

K

K

Y

Y

V

I

E

A

G

G

A

A

R

R

P

P

W

W

S

V

E

H

-

V

-

G

K

K

A

V

D

D

I

V

A

A

L

L

P

P

S

S

A
|
A

T
|
T

Q

Q

N

N

E

E

I

I

N

S

G

G

D

E

A

A

R

Q

K

V

L

L

I

I

A

N

N

A

G

G

V

C

M

K

A

F

V

I

S

A

E

E

G
|
G

A
x
S

N
|
N

M

M

P

G

S

C

T

T

P

Q

E

E

A

A

I

I

K

D

V

T

F

F

Q

E

-

A

-

H

-

R

-

T

-

A

-

N

-

A

-

G

E

A

A

A

K

A

I

I

L

W

Y

Y

A

A

P

P

G

G

K

K

A

A

N
|
N

A

A

G

G

V
|
V

S

A

V

V

S
|
S

G

G

L

L

E

E

M

M

T

A

Q

Q

N

N

S

S

I

A

K

R

L

L

G

S

W

W

S

T

S

S

E

E

V

V

D

D

E

A

K

R

L

L

K

K

S

D

I

I

M

M

-

R

-

D

-

C

-

F

K

K

N

N

I

G

H

L

A

E

A

T

C

A

V

Q

Q

E

Y

Y

G

A

T

T

E

P

E

A

N

E

G

G

Y

V

V

L

-

P

N

S

Y

L

V

V

K

T

G

G

A

S

N

N

V

I

A

A

G

G

F

F

M

T

K

K

V

V

A

A

K

A

A

A

M

M

M

K

A

D

Q

Q

G

G

binding 2-oxoglutaric acid: K77 (= K88), Q98 (= Q109), K101 (= K112), K113 (= K124), A151 (= A162), R192 (= R203), V382 (= V374), S385 (= S377)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: K121 (≠ R132), D153 (= D164), I154 (= I165), R192 (= R203), T196 (= T207), S228 (= S236), G229 (= G237), N230 (= N238), V231 (= V239), D251 (= D259), S252 (= S260), A319 (= A319), T320 (= T320), G343 (= G343), S344 (≠ A344), N345 (= N345), N378 (= N370)

5xvvB Crystal structure of forward inhibited aspergillus niger glutamate dehydrogenase with both apo- and alpha ketoglutarate bound subunits (see paper)
54% identity, 97% coverage: 14:443/445 of query aligns to 3:456/459 of 5xvvB

query
sites
5xvvB

F

L

P

P

N

H

E

E

P

P

E

E

Y

F

H

E

Q

Q

A

A

V

Y

E

K

E

E

V

L

L

A

S

S

T

T

I

L

E

E

E

N

E

S

-

T

-

L

Y

F

N

Q

K

K

H

N

P

P

E

E

F

Y

D

R

K

K

A

A

N

-

L

-

I

L

E

A

R

V

L

V

C

S

I

V

P

P

D

E

R

R

V

V

Y

I

Q

Q

F

F

R

R

V

V

T

V

W

W

V

E

D

D

K

A

G

G

N

N

V

V

Q

Q

T

V

N

N

M

R

G

G

Y

F

R

R

V

V

Q

Q

H

F

N

N

N

S

A

A

I

L

G

G

P

P

Y

Y

K
|
K

G

G

G
|
G

I

L

R

R

F

F

H

H

A

P

S

S

V

V

N

N

L

L

S

S

I

I

L

L

K

K

F

F

L

L

A

G

F

F

E

E

Q
|
Q

T

I

F

F

K

K

N

N

S

A

L

L

T

T

T

G

L

L

P

N

M

M

G

G

K
|
K

G

G

S

S

D

D

F

F

S

D

P

P

R

K

G

G

K

K

S

S

N

D

A

N

E

E

V

I

M

R

R

R

F

F

V

C

Q

V

A

S

F

F

M

M

L

T

E

E

L

L

W

C

R

K

H

H

I

I

G

G

P

A

E

D

T

T

D

D

V

V

P

P

A
|
A

G

G

D

D

I

I

G

G

V

V

G

T

G

G

R

R

E

E

V

V

G

G

F

F

M

L

F

F

G

G

M

Q

Y

Y

K

R

K

K

L

I

T

R

H

N

E

Q

F

W

T

E

G

G

T

V

F

L

T

T

G

G

K

K

G

G

R

G

E

S

F

W

G

G

S

S

L

L

I

I

R

R

P

P

E

E

A

A

T

T

G

G

Y

Y

G

G

N

V

I

V

Y

Y

F

Y

L

V

L

E

E

H

M

M

L

I

-

A

-

H

K

A

T

T

K

N

G

G

T

Q

D

E

-

S

L

F

K

K

G

G

K

K

T

R

C

V

L

A

I

I

S

S

G

G

S

S

G

G

N

N

V

V

A

A

Q

Q

Y

Y

T

A

A

A

E

L

K

K

V

V

L

I

E

E

M

L

G

G

K

S

V

V

L

V

T

S

M

L

S

S

D

D

S

S

D

Q

G

G

-

S

-

L

Y

I

I

I

Y

N

D

G

P

E

A

G

G

S

I

F

D

T

R

P

E

E

K

E

L

I

D

E

Y

L

I

I

M

A

E

Q

L

T

K

K

N

-

L

V

Y

E

R

R

G

K

R

Q

I

L

R

A

E

S

Y

I

A

V

E

G

Q

A

Y

A

P

P

-

F

-

S

-

D

-

A

-

N

G

K

V

F

K

K

Y

Y

V

I

E

A

G

G

A

A

R

R

P

P

W

W

S

V

E

H

-

V

-

G

K

K

A

V

D

D

I

V

A

A

L

L

P

P

S

S

A

A

T

T

Q

Q

N

N

E

E

I

I

N

S

G

G

D

E

A

A

R

Q

K

V

L

L

I

I

A

N

N

A

G

G

V

C

M

K

A

F

V

I

S

A

E

E

G

G

A

S

N

N

M

M

P

G

S

C

T

T

P

Q

E

E

A

A

I

I

K

D

V

T

F

F

Q

E

-

A

-

H

-

R

-

T

-

A

-

N

-

A

-

G

E

A

A

A

K

A

I

I

L

W

Y

Y

A

A

P

P

G

G

K

K

A

A

N

N

A

A

G

G

V

V

S

A

V

V

S
|
S

G

G

L

L

E

E

M

M

T

A

Q

Q

N

N

S

S

I

A

K

R

L

L

G

S

W

W

S

T

S

S

E

E

V

V

D

D

E

A

K

R

L

L

K

K

S

D

I

I

M

M

-

R

-

D

-

C

-

F

K

K

N

N

I

G

H

L

A

E

A

T

C

A

V

Q

Q

E

Y

Y

G

A

T

T

E

P

E

A

N

E

G

G

Y

V

V

L

-

P

N

S

Y

L

V

V

K

T

G

G

A

S

N

N

V

I

A

A

G

G

F

F

M

T

K

K

V

V

A

A

K

A

A

A

M

M

M

K

A

D

Q

Q

G

G

binding 2-oxoglutaric acid: K77 (= K88), G79 (= G90), Q98 (= Q109), K113 (= K124), A151 (= A162), S385 (= S377)

7ecsA Crystal structure of aspergillus terreus glutamate dehydrogenase (atgdh) complexed with malonate and NADPH (see paper)
52% identity, 97% coverage: 14:443/445 of query aligns to 4:457/460 of 7ecsA

query
sites
7ecsA

F

L

P

P

N

V

E

E

P

P

E

E

Y

F

H

E

Q

Q

A

A

V

Y

E

K

E

E

V

L

L

A

S

S

T

T

I

L

E

E

E

N

E

S

-

T

-

L

Y

F

N

Q

K

K

H

N

P

P

E

E

F

Y

D

R

K

K

A

A

N

-

L

-

I

L

E

A

R

V

L

V

C

S

I

V

P

P

D

E

R

R

V

V

Y

I

Q

Q

F

F

R

R

V

V

T

V

W

W

V

E

D

N

D

D

K

K

G

G

N

E

V

V

Q

Q

T

V

N

N

M

R

G

G

Y

F

R

R

V

V

Q

Q

H

F

N

N

N

S

A

A

I

L

G

G

P

P

Y

Y

K

K

G
|
G

I

L

R

R

F

F

H

H

A

P

S

S

V

V

N

N

L

L

S

S

I

I

L

L

K

K

F

F

L

L

A

G

F

F

E

E

Q
|
Q

T

I

F

F

K
|
K

N

N

S

A

L

L

T

T

T

G

L

L

P

N

M

M

G

G

K
|
K

G

G

S

S

D

D

F

F

S

D

P

P

R
x
K

G

G

K

K

S

S

N

D

A

S

E

E

V

I

M

R

R

R

F

F

V

C

Q

V

A

A

F

F

M

M

L

T

E

E

L

L

W

C

R

R

H

H

I

I

G

G

P

A

E

D

T

T

D

D

V

V

P

P

A

A

G

G

D
|
D

I
|
I

G

G

V

V

G

T

G

G

R

R

E

E

V

I

G

G

F

Y

M

L

F

F

G

G

M

Q

Y

Y

K

R

K

K

L

L

T

R

H

N

E

S

F

W

T

E

G

G

T

V

F

L

T

T

G

G

K

K

G

G

R

G

E

S

F

W

G

G

S

S

L

L

I

I

R
|
R

P

P

E

E

A

A

T
|
T

G

G

Y

Y

G

G

N

V

I

V

Y

Y

F

Y

L

V

L

E

E

H

M

M

L

I

-

A

-

H

K

A

T

T

K

N

G

G

T

A

D

E

-

S

L

F

K

A

G

G

K

K

T

R

C

V

L

A

I

I

S

S

G

G

S
|
S

G
|
G

N
|
N

V
|
V

A

A

Q

Q

Y

Y

T

A

A

A

E

L

K

K

V

V

L

I

E

E

M

L

G

G

K

R

V

V

L

V

T

S

M

L

S

S

D
|
D

S
|
S

D

Q

G

G

Y

S

I

L

-

I

-

V

-

K

-

D

-

T

-

A

-

K

-

D

-

S

Y

F

D

T

P

P

A

A

G

-

I

-

D

-

R

-

E

-

K

E

L

I

D

D

Y

A

I

I

M

A

E

A

L

L

K
|
K

N

-

L

V

Y

D

R

R

G

K

R

Q

I

I

R

A

E

E

-

L

-

V

-

T

-

D

-

A

Y

F

A

A

E

D

Q

K

Y

F

P

-

G

-

V

-

K

T

Y

Y

V

L

E

P

G

G

A

Q

R

R

P

P

W

W

S

V

E

H

-

V

-

G

K

A

A

V

D

D

I

V

A

A

L

L

P

P

S

S

A
|
A

T
|
T

Q

Q

N

N

E

E

I

V

N
x
S

G
|
G

D
x
E

D

E

A

A

R

Q

K

A

L

L

I

I

A

A

N

A

G

G

V

C

M

K

A

F

V

I

S

A

E

E

G
|
G

A
x
S

N
|
N

M

M

P

G

S

C

T
|
T

P

Q

E
x
A

A

A

I

I

K

D

V

A

F

F

Q

E

-

A

-

H

-

R

-

E

-

A

-

N

-

K

-

G

E

A

A

A

K

A

I

I

L

W

Y

Y

A

A

P

P

G

G

K

K

A

A

N
|
N

A

A

G

G

V

V

S

A

V

V

S

S

G

G

L

L

E

E

M

M

T

A

Q

Q

N

N

S

S

I

A

K

R

L

L

G

S

W

W

S

T

S

A

E

E

V

V

D

D

E

A

K

R

L

L

K

K

S

D

I

I

M

M

K

K

N

S

I

-

H

-

A

-

C

C

V

F

Q

Q

-

N

-

G

-

L

-

D

-

T

-

A

-

K

-

E

Y

Y

G

A

T

T

E

P

E

A

N

D

G

G

Y

I

V

L

-

P

N

S

Y

L

V

V

K

T

G

G

A

S

N

N

V

I

A

A

G

G

F

F

M

T

K

K

V

V

A

A

K

A

A

A

M

M

M

K

A

D

Q

Q

G

G

binding malonate ion: G79 (= G89), G80 (= G90), Q99 (= Q109), K102 (= K112), K114 (= K124), S326 (≠ N325), G327 (= G326), E328 (≠ D327), T350 (= T349), A352 (≠ E351)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: K122 (≠ R132), D154 (= D164), I155 (= I165), R193 (= R203), T197 (= T207), S229 (= S236), G230 (= G237), N231 (= N238), V232 (= V239), D252 (= D259), S253 (= S260), K279 (= K282), A320 (= A319), T321 (= T320), G344 (= G343), S345 (≠ A344), N346 (= N345), N379 (= N370)

7ecrA Crystal structure of aspergillus terreus glutamate dehydrogenase (atgdh) complexed with succinate and adp-ribose (see paper)
52% identity, 97% coverage: 14:443/445 of query aligns to 4:457/460 of 7ecrA

query
sites
7ecrA

F

L

P

P

N

V

E

E

P

P

E

E

Y

F

H

E

Q

Q

A

A

V

Y

E

K

E

E

V

L

L

A

S

S

T

T

I

L

E

E

E

N

E

S

-

T

-

L

Y

F

N

Q

K

K

H

N

P

P

E

E

F

Y

D

R

K

K

A

A

N

-

L

-

I

L

E

A

R

V

L

V

C

S

I

V

P

P

D

E

R

R

V

V

Y

I

Q

Q

F

F

R

R

V

V

T

V

W

W

V

E

D

N

D

D

K

K

G

G

N

E

V

V

Q

Q

T

V

N

N

M

R

G

G

Y

F

R

R

V

V

Q

Q

H

F

N

N

N

S

A

A

I

L

G

G

P

P

Y

Y

K

K

G

G

I

L

R

R

F

F

H

H

A

P

S

S

V

V

N

N

L

L

S

S

I

I

L

L

K

K

F

F

L

L

A

G

F

F

E

E

Q

Q

T

I

F

F

K

K

N

N

S

A

L

L

T

T

T

G

L

L

P

N

M

M

G

G

K

K

G

G

S

S

D

D

F

F

S

D

P

P

R
x
K

G

G

K

K

S

S

N

D

A

S

E

E

V

I

M

R

R

R

F

F

V

C

Q

V

A

A

F

F

M

M

L

T

E

E

L

L

W

C

R

R

H

H

I

I

G

G

P

A

E

D

T

T

D

D

V

V

P

P

A

A

G

G

D
|
D

I
|
I

G

G

V

V

G

T

G

G

R

R

E

E

V

I

G

G

F

Y

M

L

F

F

G

G

M

Q

Y

Y

K

R

K

K

L

L

T

R

H

N

E

S

F

W

T

E

G

G

T

V

F

L

T

T

G

G

K

K

G

G

R

G

E

S

F

W

G

G

S

S

L

L

I

I

R

R

P

P

E

E

A

A

T

T

G

G

Y

Y

G

G

N

V

I

V

Y

Y

F

Y

L

V

L

E

E

H

M

M

L

I

-

A

-

H

K

A

T

T

K

N

G

G

T

A

D

E

-

S

L

F

K

A

G

G

K

K

T

R

C

V

L

A

I

I

S

S

G

G

S
|
S

G
|
G

N
|
N

V
|
V

A

A

Q

Q

Y

Y

T

A

A

A

E

L

K

K

V

V

L

I

E

E

M

L

G

G

K

R

V

V

L

V

T

S

M

L

S

S

D
|
D

S
|
S

D

Q

G

G

Y

S

I

L

-

I

-

V

-

K

-

D

-

T

-

A

-

K

-

D

-

S

Y

F

D

T

P

P

A

A

G

-

I

-

D

-

R

-

E

-

K

E

L

I

D

D

Y

A

I

I

M

A

E

A

L

L

K
|
K

N

-

L

V

Y

D

R

R

G

K

R

Q

I

I

R

A

E

E

-

L

-

V

-

T

-

D

-

A

Y

F

A

A

E

D

Q

K

Y

F

P

-

G

-

V

-

K

T

Y

Y

V

L

E

P

G

G

A

Q

R

R

P

P

W

W

S

V

E

H

-

V

-

G

K

A

A

V

D

D

I

V

A

A

L

L

P

P

S

S

A
|
A

T
|
T

Q

Q

N

N

E

E

I

V

N

S

G

G

D

E

A

A

R

Q

K

A

L

L

I

I

A

A

N

A

G

G

V

C

M

K

A

F

V

I

S

A

E

E

G
|
G

A
x
S

N
|
N

M

M

P

G

S

C

T

T

P

Q

E

A

A

A

I

I

K

D

V

A

F

F

Q

E

-

A

-

H

-

R

-

E

-

A

-

N

-

K

-

G

E

A

A

A

K

A

I

I

L

W

Y

Y

A

A

P

P

G

G

K

K

A

A

N
|
N

A

A

G

G

V

V

S

A

V

V

S

S

G

G

L

L

E

E

M

M

T

A

Q

Q

N

N

S

S

I

A

K

R

L

L

G

S

W

W

S

T

S

A

E

E

V

V

D

D

E

A

K

R

L

L

K

K

S

D

I

I

M

M

K

K

N

S

I

-

H

-

A

-

C

C

V

F

Q

Q

-

N

-

G

-

L

-

D

-

T

-

A

-

K

-

E

Y

Y

G

A

T

T

E

P

E

A

N

D

G

G

Y

I

V

L

-

P

N

S

Y

L

V

V

K

T

G

G

A

S

N

N

V

I

A

A

G

G

F

F

M

T

K

K

V

V

A

A

K

A

A

A

M

M

M

K

A

D

Q

Q

G

G

binding [(2r,3r,4r,5r)-5-(6-amino-9h-purin-9-yl)-3-hydroxy-4-(phosphonooxy)tetrahydrofuran-2-yl]methyl [(2r,3s,4r,5r)-3,4,5-trihydroxytetrahydrofuran-2-yl]methyl dihydrogen diphosphate: K122 (≠ R132), D154 (= D164), I155 (= I165), S229 (= S236), G230 (= G237), N231 (= N238), V232 (= V239), D252 (= D259), S253 (= S260), K279 (= K282), A320 (= A319), T321 (= T320), G344 (= G343), S345 (≠ A344), N346 (= N345), N379 (= N370)

P78804 NADP-specific glutamate dehydrogenase; NADP-GDH; NADP-dependent glutamate dehydrogenase; EC 1.4.1.4 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
52% identity, 96% coverage: 15:443/445 of query aligns to 4:448/451 of P78804

query
sites
P78804

P

P

N

Y

E

E

P

P

E

E

Y

F

H

Q

Q

Q

A

A

V

Y

E

K

E

E

V

I

L

V

S

G

T

S

I

I

E

E

E

S

-

K

-

L

Y

F

N

E

K

V

H

H

P

P

E

E

F

L

D

K

K

R

A

-

N

-

L

V

I

L

E

P

R

I

L

I

C

S

I

I

P

P

D

E

R

R

V

V

Y

L

Q

E

F

F

R

R

V

V

T

T

W

W

V

E

D

D

K

K

G

G

N

N

V

C

Q

R

T

V

N

N

M

T

G

G

Y

Y

R

R

V

V

Q

Q

H

F

N

N

N

S

A

A

I

L

G

G

P

P

Y

Y

K

K

G

G

I

L

R

R

F

F

H

H

A

P

S

S

V

V

N

N

L

L

S

S

I

I

L

L

K

K

F

F

L

L

A

G

F

F

E

E

Q

Q

T

I

F

F

K

K

N

N

S

A

L

L

T

T

T

G

L

L

P

P

M

M

G

G

K

K

G

G

S

S

D

D

F

F

S

D

P

P

R

K

G

G

K

K

S

S

N

D

A

N

E

E

V

I

M

R

R

R

F

F

V

S

Q

Q

A

A

F

F

M

M

L

R

E

Q

L

L

W

F

R

R

H

Y

I

I

G

G

P

P

E

Q

T

T

D

D

V

V

P

P

A

A

G

G

D

D

I

I

G

G

V

V

G

T

G

G

R

F

E

V

V

V

G

M

F

H

M

M

F

F

G

G

M

E

Y

Y

K

K

K

R

L

L

T

R

H

N

E

E

F

Y

T

S

G

G

T

V

F

V

T

T

G

G

K

K

G

H

R

M

E

L

F

T

G

G

S

S

L

N

I

I

R

R

P

P

E

E

A

A

T

T

G

G

Y

Y

G

G

N

V

I

V

Y

Y

F

Y

L

V

L

K

E

H

M

M

L

I

-

E

-

H

K

R

T

T

K

K

G

G

T

A

D

E

-

T

L

L

K

K

G

G

K

K

T

R

C

V

L

A

I

I

S

S

G

G

S

S

G

G

N

N

V

V

A

A

Q

Q

Y

Y

T

A

A

A

E

L

K

K

V

C

L

I

E

Q

M

E

G

G

G

A

K

I

V

V

L

K

T

S

M

I

S

S

D

D

S
|
S

D

K

G

G

Y

V

I

L

Y

I

D

A

P

K

A

T

-

A

-

E

G

G

I

L

D

V

R

P

E

E

K

E

L

I

D

H

Y

E

I

I

M

M

E

A

L

L

K

K

N

E

L

-

Y

K

R

R

G

A

R

S

I

I

R

A

E

D

Y

S

A

A

E

S

Q

L

Y

C

P

K

G

K

V

H

K

H

Y

Y

V

I

E

A

G

G

A

A

R

R

P

P

W

W

S

T

E

N

-

V

-

G

K

E

A

I

D

D

I

I

A

A

L

L

P

P

S

C

A

A

T

T

Q

Q

N

N

E

E

I

V

N

S

G

G

D

E

A

A

R

A

K

A

L

L

I

I

A

K

N

Q

G

G

V

C

M

R

A

Y

V

V

S

A

E

E

G

G

A

S

N

N

M

M

P

G

S

S

T

S

P

A

E

E

A

A

I

V

K

E

V

V

F

F

Q

E

E

K

A

S

K

R

-

A

-

S

-

G

-

E

-

G

I

C

L

W

Y

L

A

A

P

P

G

G

K

K

A

A

N

N

A

A

G

G

V

V

S

A

V

V

S

S

G

G

L

L

E

E

M

M

T

A

Q

Q

N

N

S

A

I

Q

K

F

L

S

G

T

W

W

S

T

S

H

E

A

E

E

V

V

D

D

E

A

K

K

L

L

K

A

S

G

I

I

M

M

K

Q

N

N

I

I

H

F

A

E

A

Q

C

S

V

T

Q

D

Y

V

G

A

T

S

E

K

-

Y

-

C

-

D

-

S

-

G

E

S

N

N

G

N

Y

I

V

P

N

S

Y

L

V

V

K

D

G

G

A

A

N

N

V

I

A

A

G

G

F

F

M

L

K

K

V

V

A

A

K

T

A

A

M

M

M

Q

A

A

Q

V

G

G

S252 (= S260) modified: Phosphoserine

8xd5A Cryo-em structure of glutamate dehydrogenase from thermococcus profundus in complex with NADP and glu in the steady stage of reaction (see paper)
36% identity, 89% coverage: 47:443/445 of query aligns to 28:415/419 of 8xd5A

query
sites
8xd5A

I

L

E

E

R

W

L

L

C

K

I

K

P

P

D

M

R

R

V

I

Y

V

Q

E

F

V

R

S

V

V

T

P

W

I

V

E

D

M

D

D

K

D

G

G

N

S

V

V

Q

K

T

V

N

F

M

T

G

G

Y

F

R

R

V

V

Q

Q

H

H

N

N

N

W

A

A

I

R

G

G

P

P

Y

T

K
|
K

G

G

I

I

R
|
R

F

W

H

H

A

P

S

A

V

E

N

T

L

L

S

S

I

T

L

V

K

K

F

A

L

L

A

A

F

T

E

W

Q
x
M

T

T

F

W

K

K

N

V

S

A

L

V

T

V

T

D

L

L

P

P

M

Y

G

G

K
|
K

G

G

S

I

D

I

F

V

S

N

P

P

R

K

G

E

K

L

S

S

N

E

A

R

E

E

V

Q

M

E

R

R

F

L

V

A

Q

R

A

A

F

Y

M

I

L

R

E

A

L

V

W

Y

R

D

H

V

I

I

G

G

P

P

E

W

T

T

D

D

V

I

P

P

A
|
A

G

P

D
|
D

I
x
V

G
x
Y

V

T

G

N

G

P

R

K

E

I

V

M

G

G

F

W

M

M

F

M

G

D

M

E

Y

Y

K

E

K

T

L

I

T

M

H

R

E

R

-

K

-

G

-

P

F

A

T

F

G

G

T

V

F

I

T

T

G

G

K

K

G

P

R

L

E

S

F

I

G

G

S

S

L

L

I

G

R

R

P

G

E

T

A

A

T
|
T

G

A

Y

Q

G

G

N

A

I

I

Y

F

F

T

L

I

L

R

E

E

M

A

L

A

K

K

T

A

K

L

G

G

T

I

D

D

L

L

K

K

G

G

K

K

T

K

C

I

L

A

I

V

S

Q

G

G

S
x
Y

G
|
G

N
|
N

V
x
A

A

G

Q

Y

Y

Y

T

T

A

A

E

K

K

L

V

A

L

K

E

E

-

Q

M

L

G

G

G

M

K

T

V

V

L

V

T

A

M

V

S

S

D
|
D

S
|
S

D

R

G

G

Y

G

I

I

Y

Y

D

N

P

P

A

D

G

G

I

L

D

D

R

P

E

-

K

-

L

-

D

D

Y

E

I

V

M

L

E

K

L

W

K
|
K

N

R

L

E

Y

H

R

-

G

G

R

S

I

V

R

K

E

D

Y

-

A

-

E

-

Q

-

Y

F

P

P

G

G

V

A

K

T

Y

N

V

I

E

T

G
x
N

A

E

R

E

P

L

W

L

S

E

E

L

K

E

A

V

D

D

I

V

A

L

L

A

P

P

S
x
A

A
|
A

T
x
I

Q

E

N

E

E

V

I

I

N

T

G

E

D

K

D

N

A

A

R

D

K

N

L

I

I

K

A

A

N

K

G

-

V

-

M

-

A

I

V

V

S

A

E

E

G

V

A

A

N
|
N

M

G

P

P

S

V

T

T

P

P

E

E

A

A

I

D

K

D

V

I

F

L

Q

R

E

E

A

K

K

G

I

I

L

L

Y

Q

A

I

P

P

G

D

K

F

A

L

A

C

N
|
N

A

A

G

G

V

V

S

T

V

V

S
|
S

G

Y

L

F

E

E

M

W

T

V

Q

Q

N

N

S

I

I

N

K

G

L

Y

G

Y

W

W

S

T

S

E

E

E

V

V

D

R

E

E

K

K

L

L

K

D

S

K

I

K

M

M

K

T

N

K

I

-

H

-

A

A

A

F

C

W

V

E

Q

V

Y

Y

G

N

T

T

E

H

E

K

N

D

G

K

Y

N

V

I

N

H

Y

M

V

R

K

D

G

A

A

A

N

Y

V

V

A

V

G

A

F

V

M

S

K

R

V

V

A

Y

K

Q

A

A

M

M

M

K

A

D

Q

R

G

G

binding gamma-l-glutamic acid: K69 (= K88), M90 (≠ Q109), K105 (= K124), A143 (= A162), D145 (= D164), S351 (= S377)
binding nadp nicotinamide-adenine-dinucleotide phosphate: R73 (= R92), D145 (= D164), V146 (≠ I165), Y147 (≠ G166), T191 (= T207), Y220 (≠ S236), G221 (= G237), N222 (= N238), A223 (≠ V239), D244 (= D259), S245 (= S260), K264 (= K282), N281 (≠ G304), A295 (≠ S318), A296 (= A319), I297 (≠ T320), N319 (= N345), N344 (= N370)

8xcsA Cryo-em structure of glutamate dehydrogenase from thermococcus profundus in complex with NADPH, akg and nh4 in the initial stage of reaction (see paper)
36% identity, 89% coverage: 47:443/445 of query aligns to 27:414/418 of 8xcsA

query
sites
8xcsA

I

L

E

E

R

W

L

L

C

K

I

K

P

P

D

M

R

R

V

I

Y

V

Q

E

F

V

R

S

V

V

T

P

W

I

V

E

D

M

D

D

K

D

G

G

N

S

V

V

Q

K

T

V

N

F

M

T

G

G

Y

F

R

R

V

V

Q

Q

H

H

N

N

N

W

A

A

I

R

G

G

P

P

Y

T

K
|
K

G

G

G
|
G

I

I

R
|
R

F

W

H

H

A

P

S

A

V

E

N

T

L

L

S

S

I

T

L

V

K

K

F

A

L

L

A

A

F

T

E

W

Q
x
M

T

T

F

W

K
|
K

N

V

S

A

L

V

T

V

T

D

L

L

P

P

M

Y

G

G

K
|
K

G

G

S

I

D

I

F

V

S

N

P

P

R
x
K

G

E

K

L

S

S

N

E

A

R

E

E

V

Q

M

E

R

R

F

L

V

A

Q

R

A

A

F

Y

M

I

L

R

E

A

L

V

W

Y

R

D

H

V

I

I

G

G

P

P

E

W

T

T

D

D

V

I

P

P

A
|
A

G
x
P

D
|
D

I
x
V

G
x
Y

V

T

G

N

G

P

R

K

E

I

V

M

G

G

F

W

M

M

F

M

G

D

M

E

Y

Y

K

E

K

T

L

I

T

M

H

R

E

R

-

K

-

G

-

P

F

A

T

F

G

G

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F

I

T

T

G

G

K

K

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R

L

E

S

F

I

G

G

S

S

L

L

I

G

R

R

P

G

E

T

A

A

T
|
T

G

A

Y

Q

G

G

N

A

I

I

Y

F

F

T

L

I

L

R

E

E

M

A

L

A

K

K

T

A

K

L

G

G

T

I

D

D

L

L

K

K

G

G

K

K

T

K

C

I

L

A

I

V

S

Q

G

G

S
x
Y

G
|
G

N
|
N

V
x
A

A

G

Q

Y

Y

Y

T

T

A

A

E

K

K

L

V

A

L

K

E

E

-

Q

M

L

G

G

G

M

K

T

V

V

L

V

T

A

M

V

S

S

D
|
D

S
|
S

D

R

G

G

Y

G

I

I

Y

Y

D

N

P

P

A

D

G

G

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L

D

D

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-

K

-

L

-

D

D

Y

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M

L

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K

L

W

K
|
K

N

R

L

E

Y

H

R

-

G

G

R

S

I

V

R

K

E

D

Y

-

A

-

E

-

Q

-

Y

F

P

P

G

G

V

A

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N

V

I

E

T

G

N

A

E

R

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P

L

W

L

S

E

E

L

K

E

A

V

D

D

I

V

A

L

L

A

P

P

S

A

A
|
A

T
x
I

Q

E

N

E

E

V

I

I

N

T

G

E

D

K

D

N

A

A

R

D

K

N

L

I

I

K

A

A

N

K

G

-

V

-

M

-

A

I

V

V

S

A

E

E

G

V

A

A

N
|
N

M

G

P

P

S

V

T

T

P

P

E

E

A

A

I

D

K

D

V

I

F

L

Q

R

E

E

A

K

K

G

I

I

L

L

Y

Q

A

I

P

P

G

D

K

F

A

L

A

C

N

N

A

A

G

G

G
|
G

V

V

S

T

V

V

S
|
S

G

Y

L

F

E

E

M

W

T

V

Q

Q

N

N

S

I

I

N

K

G

L

Y

G

Y

W

W

S

T

S

E

E

E

V

V

D

R

E

E

K

K

L

L

K

D

S

K

I

K

M

M

K

T

N

K

I

-

H

-

A

A

A

F

C

W

V

E

Q

V

Y

Y

G

N

T

T

E

H

E

K

N

D

G

K

Y

N

V

I

N

H

Y

M

V

R

K

D

G

A

A

A

N

Y

V

V

A

V

G

A

F

V

M

S

K

R

V

V

A

Y

K

Q

A

A

M

M

M

K

A

D

Q

R

G

G

binding 2-oxoglutaric acid: K68 (= K88), G70 (= G90), M89 (≠ Q109), K92 (= K112), K104 (= K124), A142 (= A162), D144 (= D164), G346 (= G373), S350 (= S377)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: R72 (= R92), K112 (≠ R132), P143 (≠ G163), D144 (= D164), V145 (≠ I165), Y146 (≠ G166), T190 (= T207), Y219 (≠ S236), G220 (= G237), N221 (= N238), A222 (≠ V239), D243 (= D259), S244 (= S260), K263 (= K282), A295 (= A319), I296 (≠ T320), N318 (= N345)

8xcoA Cryo-em structure of glutamate dehydrogenase from thermococcus profundus incorporating NADPH in the initial stage of reaction (see paper)
36% identity, 89% coverage: 47:443/445 of query aligns to 25:412/416 of 8xcoA

query
sites
8xcoA

I

L

E

E

R

W

L

L

C

K

I

K

P

P

D

M

R

R

V

I

Y

V

Q

E

F

V

R

S

V

V

T

P

W

I

V

E

D

M

D

D

K

D

G

G

N

S

V

V

Q

K

T

V

N

F

M

T

G

G

Y

F

R

R

V

V

Q

Q

H

H

N

N

N

W

A

A

I

R

G

G

P

P

Y

T

K

K

G

G

I

I

R

R

F

W

H

H

A

P

S

A

V

E

N

T

L

L

S

S

I

T

L

V

K

K

F

A

L

L

A

A

F

T

E

W

Q

M

T

T

F

W

K

K

N

V

S

A

L

V

T

V

T

D

L

L

P

P

M

Y

G

G

K

K

G

G

S

I

D

I

F

V

S

N

P

P

R

K

G

E

K

L

S

S

N

E

A

R

E

E

V

Q

M

E

R

R

F

L

V

A

Q

R

A

A

F

Y

M

I

L

R

E

A

L

V

W

Y

R

D

H

V

I

I

G

G

P

P

E

W

T

T

D

D

V

I

P

P

A

A

G

P

D

D

I

V

G

Y

V

T

G

N

G

P

R

K

E

I

V

M

G

G

F

W

M

M

F

M

G

D

M

E

Y

Y

K

E

K

T

L

I

T

M

H

R

E

R

-

K

-

G

-

P

F

A

T

F

G

G

T

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F

I

T

T

G

G

K

K

G

P

R

L

E

S

F

I

G

G

S

S

L

L

I

G

R

R

P

G

E

T

A

A

T
|
T

G

A

Y

Q

G

G

N

A

I

I

Y

F

F

T

L

I

L

R

E

E

M

A

L

A

K

K

T

A

K

L

G

G

T

I

D

D

L

L

K

K

G

G

K

K

T

K

C

I

L

A

I

V

S

Q

G

G

S
x
Y

G
|
G

N
|
N

V
x
A

A

G

Q

Y

Y

Y

T

T

A

A

E

K

K

L

V

A

L

K

E

E

-

Q

M

L

G

G

G

M

K

T

V

V

L

V

T

A

M

V

S

S

D
|
D

S
|
S

D

R

G

G

Y

G

I

I

Y

Y

D

N

P

P

A

D

G

G

I

L

D

D

R

P

E

-

K

-

L

-

D

D

Y

E

I

V

M

L

E

K

L

W

K
|
K

N

R

L

E

Y

H

R

-

G

G

R

S

I

V

R

K

E

D

Y

-

A

-

E

-

Q

-

Y

F

P

P

G

G

V

A

K

T

Y

N

V

I

E

T

G

N

A

E

R

E

P

L

W

L

S

E

E

L

K

E

A

V

D

D

I

V

A

L

L

A

P

P

S

A

A

A

T
x
I

Q

E

N

E

E

V

I

I

N

T

G

E

D

K

D

N

A

A

R

D

K

N

L

I

I

K

A

A

N

K

G

-

V

-

M

-

A

I

V

V

S

A

E

E

G

V

A

A

N

N

M

G

P

P

S

V

T

T

P

P

E

E

A

A

I

D

K

D

V

I

F

L

Q

R

E

E

A

K

K

G

I

I

L

L

Y

Q

A

I

P

P

G

D

K

F

A

L

A

C

N

N

A

A

G

G

V

V

S

T

V

V

S

S

G

Y

L

F

E

E

M

W

T

V

Q

Q

N

N

S

I

I

N

K

G

L

Y

G

Y

W

W

S

T

S

E

E

E

V

V

D

R

E

E

K

K

L

L

K

D

S

K

I

K

M

M

K

T

N

K

I

-

H

-

A

A

A

F

C

W

V

E

Q

V

Y

Y

G

N

T

T

E

H

E

K

N

D

G

K

Y

N

V

I

N

H

Y

M

V

R

K

D

G

A

A

A

N

Y

V

V

A

V

G

A

F

V

M

S

K

R

V

V

A

Y

K

Q

A

A

M

M

M

K

A

D

Q

R

G

G

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: T188 (= T207), Y217 (≠ S236), G218 (= G237), N219 (= N238), A220 (≠ V239), D241 (= D259), S242 (= S260), K261 (= K282), I294 (≠ T320)

8zneA Cryo-em structure of w89f mutated glutamate dehydrogenase from thermococcus profundus in complex with NADP and glu in the steady stage of reaction
36% identity, 89% coverage: 47:443/445 of query aligns to 25:412/416 of 8zneA

query
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binding glutamic acid: M87 (≠ Q109), K90 (= K112), A140 (= A162), D142 (= D164), S348 (= S377)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G216 (= G235), Y217 (≠ S236), G218 (= G237), N219 (= N238), A220 (≠ V239), D241 (= D259), S242 (= S260), A293 (= A319), I294 (≠ T320)

P39633 Catabolic NAD-specific glutamate dehydrogenase RocG; NAD-GDH; Glutamate dehydrogenase; GlutDH; Trigger enzyme RocG; EC 1.4.1.2 from Bacillus subtilis (strain 168) (see 2 papers)
33% identity, 90% coverage: 45:443/445 of query aligns to 37:422/424 of P39633

query
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P39633

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E93 (≠ I101) mutation to K: Reduces the affinity for glutamate and ammonium.
D122 (≠ S130) mutation to N: Unable to control gltAB expression via an inhibitory interactions with the transcriptional regulator GltC. Reduces the affinity for glutamate and ammonium.
Q144 (≠ R152) mutation to R: Increase of thermostability 20 degrees Celsius higher than that of the wild-type.
Y158 (≠ G166) mutation to H: Reduces the affinity for glutamate and ammonium.
S234 (≠ Q241) mutation to R: Reduces the affinity for glutamate and ammonium.
A324 (≠ G343) mutation to R: No effect.

Sites not aligning to the query:

27 E→F: Increase of thermostability 8 degrees Celsius higher than that of the wild-type.

Query Sequence

>WP_009123266.1 NCBI__GCF_900129655.1:WP_009123266.1
MNAAKVLEDLKRRFPNEPEYHQAVEEVLSTIEEEYNKHPEFDKANLIERLCIPDRVYQFR
VTWVDDKGNVQTNMGYRVQHNNAIGPYKGGIRFHASVNLSILKFLAFEQTFKNSLTTLPM
GGGKGGSDFSPRGKSNAEVMRFVQAFMLELWRHIGPETDVPAGDIGVGGREVGFMFGMYK
KLTHEFTGTFTGKGREFGGSLIRPEATGYGNIYFLLEMLKTKGTDLKGKTCLISGSGNVA
QYTAEKVLEMGGKVLTMSDSDGYIYDPAGIDREKLDYIMELKNLYRGRIREYAEQYPGVK
YVEGARPWSEKADIALPSATQNEINGDDARKLIANGVMAVSEGANMPSTPEAIKVFQEAK
ILYAPGKAANAGGVSVSGLEMTQNSIKLGWSSEEVDEKLKSIMKNIHAACVQYGTEENGY
VNYVKGANVAGFMKVAKAMMAQGIL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory