SitesBLAST
Comparing WP_009123490.1 NCBI__GCF_000195635.1:WP_009123490.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6ilsB Structure of arabidopsis thaliana ribokinase complexed with ribose and atp (see paper)
30% identity, 90% coverage: 23:288/296 of query aligns to 39:301/313 of 6ilsB
- binding adenosine-5'-triphosphate: N189 (= N177), K225 (≠ T214), G227 (= G216), I246 (vs. gap), A248 (≠ K235), A257 (= A244), G258 (= G245), F261 (= F248), A286 (= A274), S287 (≠ Y275)
- binding alpha-D-ribofuranose: G40 (= G24), K41 (≠ A25), N44 (= N28), E144 (= E127), D259 (= D246)
Sites not aligning to the query:
A1A6H3 Ribokinase; AtRBSK; RK; EC 2.7.1.15 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
30% identity, 90% coverage: 23:288/296 of query aligns to 105:367/379 of A1A6H3
Sites not aligning to the query:
- 1:74 mutation Missing: Loss of ribokinase activity.
3ih0A Crystal structure of an uncharacterized sugar kinase ph1459 from pyrococcus horikoshii in complex with amp-pnp
25% identity, 85% coverage: 4:256/296 of query aligns to 2:256/304 of 3ih0A
Sites not aligning to the query:
3gbuA Crystal structure of an uncharacterized sugar kinase ph1459 from pyrococcus horikoshii in complex with atp
25% identity, 85% coverage: 4:256/296 of query aligns to 1:255/302 of 3gbuA
Sites not aligning to the query:
5eynA Crystal structure of fructokinase from vibrio cholerae o395 in fructose, adp, beryllium trifluoride and calcium ion bound form
26% identity, 94% coverage: 9:287/296 of query aligns to 8:296/306 of 5eynA
- active site: G246 (= G243), A247 (= A244), G248 (= G245), D249 (= D246)
- binding adenosine-5'-diphosphate: H91 (≠ M85), T217 (= T214), G219 (= G216), A220 (≠ T217), A238 (≠ K235), V239 (= V236), T244 (= T241), G246 (= G243), A247 (= A244), G248 (= G245), F251 (= F248), N279 (≠ V270), G282 (≠ S273), A283 (= A274)
- binding beryllium trifluoride ion: G246 (= G243), G248 (= G245), D249 (= D246)
- binding beta-D-fructofuranose: D9 (≠ E10), D13 (= D14), G28 (= G24), A29 (= A25), N32 (= N28), F96 (≠ Y90), F98 (≠ I92), R159 (= R155), D249 (= D246)
4wjmA Crystal structure of fructokinase from brucella abortus 2308 with bound amppnp
24% identity, 95% coverage: 4:284/296 of query aligns to 7:306/312 of 4wjmA
- active site: G252 (= G243), A253 (= A244), G254 (= G245), D255 (= D246)
- binding phosphoaminophosphonic acid-adenylate ester: T223 (= T214), G225 (= G216), A226 (≠ T217), G228 (= G219), G252 (= G243), A253 (= A244), G254 (= G245), V257 (≠ F248), V292 (= V270), A295 (≠ S273)
5yggA Crystal structure of fructokinase double-mutant (t261c-h108c) from vibrio cholerae o395 in fructose, adp and potassium ion bound form (see paper)
25% identity, 94% coverage: 9:287/296 of query aligns to 12:300/310 of 5yggA
- binding adenosine-5'-diphosphate: K188 (= K175), T221 (= T214), G223 (= G216), A242 (≠ K235), V243 (= V236), F255 (= F248), N283 (≠ V270), G286 (≠ S273), A287 (= A274)
- binding beta-D-fructofuranose: D13 (≠ E10), D17 (= D14), G32 (= G24), A33 (= A25), F100 (≠ Y90), F102 (≠ I92), R163 (= R155), D253 (= D246)
Q8ZKR2 Aminoimidazole riboside kinase; AIRs kinase; EC 2.7.1.223 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
25% identity, 97% coverage: 1:287/296 of query aligns to 4:298/319 of Q8ZKR2
- D16 (= D14) binding 5-amino-1-(beta-D-ribosyl)imidazole
- G31 (= G24) binding 5-amino-1-(beta-D-ribosyl)imidazole
- Y101 (= Y90) binding 5-amino-1-(beta-D-ribosyl)imidazole
- R162 (= R155) binding 5-amino-1-(beta-D-ribosyl)imidazole
- A180 (≠ M168) binding K(+)
- A181 (≠ R169) binding K(+)
- A183 (≠ C171) binding K(+)
- G213 (vs. gap) binding K(+)
- D246 (= D240) binding K(+)
- T248 (≠ V242) binding K(+)
- D252 (= D246) binding 5-amino-1-(beta-D-ribosyl)imidazole
- A287 (≠ V276) binding K(+)
- A290 (≠ Q279) binding K(+)
- G292 (= G281) binding K(+)
P55263 Adenosine kinase; AK; Adenosine 5'-phosphotransferase; EC 2.7.1.20 from Homo sapiens (Human) (see 4 papers)
29% identity, 48% coverage: 148:288/296 of query aligns to 208:360/362 of P55263
- D235 (≠ N172) to A: in HMAKD; the mutant shows some residual adenosine kinase activity; dbSNP:rs397514453
- Q306 (≠ K235) binding adenosine
- D317 (= D246) active site, Proton acceptor
- A318 (≠ S247) to E: in HMAKD; complete loss of adenosine kinase activity; dbSNP:rs397514452
Sites not aligning to the query:
- 8:16 Nuclear localization signal
- 11:12 mutation KK->AA,AD: Abolishes nuclear localization.
- 30 G → E: in HMAKD; the mutant shows some residual adenosine kinase activity; dbSNP:rs397514454
- 35 binding adenosine
- 49 binding Mg(2+)
- 77 modified: Phosphotyrosine
- 147 binding Mg(2+)
- 148 binding Mg(2+)
2i6aA Human adenosine kinase in complex with 5'-deoxy-5-iodotubercidin (see paper)
29% identity, 48% coverage: 148:288/296 of query aligns to 189:341/343 of 2i6aA
Sites not aligning to the query:
1tz3A Crystal structure of aminoimidazole riboside kinase complexed with aminoimidazole riboside (see paper)
24% identity, 97% coverage: 2:287/296 of query aligns to 1:287/299 of 1tz3A
- active site: C24 (vs. gap), F88 (≠ M85), G238 (= G243), A239 (= A244), G240 (= G245), D241 (= D246)
- binding 5-aminoimidazole ribonucleoside: D8 (≠ E10), S10 (≠ L12), D12 (= D14), G27 (= G24), L83 (≠ V80), F88 (≠ M85), Y90 (= Y90), R151 (= R155), M154 (≠ F158), D241 (= D246)
1bx4A Structure of human adenosine kinase at 1.50 angstroms (see paper)
29% identity, 48% coverage: 148:288/296 of query aligns to 188:340/342 of 1bx4A
- active site: G294 (= G243), A295 (= A244), G296 (= G245), D297 (= D246)
- binding adenosine: T262 (= T214), G264 (= G216), R265 (≠ T217), Q286 (≠ K235), I289 (≠ V238), N293 (≠ V242), D297 (= D246), A324 (≠ S273), I328 (≠ C277)
Sites not aligning to the query:
1tz6A Crystal structure of aminoimidazole riboside kinase from salmonella enterica complexed with aminoimidazole riboside and atp analog (see paper)
24% identity, 97% coverage: 2:287/296 of query aligns to 1:287/297 of 1tz6A
- active site: C24 (vs. gap), F88 (≠ M85), G238 (= G243), A239 (= A244), G240 (= G245), D241 (= D246)
- binding phosphomethylphosphonic acid adenylate ester: N149 (= N153), K176 (= K175), E181 (= E180), S209 (≠ T214), G211 (= G216), A212 (≠ T217), G214 (= G219), A239 (= A244), G240 (= G245), F243 (= F248), N270 (≠ V270), G273 (≠ S273), A274 (= A274)
- binding 5-aminoimidazole ribonucleoside: D8 (≠ E10), D12 (= D14), G27 (= G24), F88 (≠ M85), Y90 (= Y90), R151 (= R155), M154 (≠ F158), D241 (= D246)
2c49A Crystal structure of methanocaldococcus jannaschii nucleoside kinase - an archaeal member of the ribokinase family (see paper)
24% identity, 92% coverage: 16:287/296 of query aligns to 32:286/299 of 2c49A
- active site: G242 (= G243), A243 (= A244), G244 (= G245), D245 (= D246)
- binding adenosine: G40 (= G23), G41 (= G24), N45 (= N28), A97 (≠ G76), T109 (≠ P88), F111 (≠ Y90), Q161 (= Q146), D162 (≠ N153), D245 (= D246)
- binding phosphoaminophosphonic acid-adenylate ester: Q107 (≠ G86), N184 (= N177), T212 (= T214), G214 (= G216), G217 (= G219), C231 (≠ K235), T241 (≠ V242), A243 (= A244), A272 (≠ S273)
Sites not aligning to the query:
Q57849 Nucleoside kinase; NK; ATP-dependent nucleoside monophosphokinase; Cytidine kinase; Guanosine-inosine kinase; EC 2.7.1.213; EC 2.7.1.73 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
24% identity, 92% coverage: 16:287/296 of query aligns to 34:288/302 of Q57849
- G43 (= G24) binding substrate
- N47 (= N28) binding substrate
- Q109 (≠ G86) binding ATP
- TFF 111:113 (≠ PCY 88:90) binding substrate
- Q163 (= Q146) binding substrate
- N186 (= N177) binding ATP
- TKGSKG 214:219 (≠ TCGTNG 214:219) binding ATP
- D247 (= D246) binding substrate
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 17 binding substrate
- 33 binding substrate
P55264 Adenosine kinase; AK; Adenosine 5'-phosphotransferase; EC 2.7.1.20 from Mus musculus (Mouse) (see paper)
28% identity, 48% coverage: 148:288/296 of query aligns to 207:359/361 of P55264
- S272 (≠ L208) mutation to A: No effect on in vitro phosphorylation by PKC.
- S328 (= S258) mutation to A: No effect on in vitro phosphorylation by PKC.
Sites not aligning to the query:
- 48 S→A: No effect on in vitro phosphorylation by PKC.
- 85 S→A: No effect on in vitro phosphorylation by PKC.
5kb5A Crystal structure of the adenosine kinase from mus musculus in complex with adenosine and adenosine-diphosphate
28% identity, 48% coverage: 148:288/296 of query aligns to 188:340/341 of 5kb5A
- active site: G294 (= G243), A295 (= A244), G296 (= G245), D297 (= D246)
- binding adenosine: N293 (≠ V242), D297 (= D246)
- binding adenosine-5'-diphosphate: N220 (= N177), T262 (= T214), G264 (= G216), R265 (≠ T217), V281 (≠ Y230), Q286 (≠ K235), I289 (≠ V238), A295 (= A244), G296 (= G245), A324 (≠ S273), I328 (≠ C277)
Sites not aligning to the query:
2varA Crystal structure of sulfolobus solfataricus 2-keto-3-deoxygluconate kinase complexed with 2-keto-3-deoxygluconate (see paper)
28% identity, 29% coverage: 197:281/296 of query aligns to 208:292/311 of 2varA