SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_009126518.1 NCBI__GCF_000195635.1:WP_009126518.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6yu5A Crystal structure of mhst in complex with l-valine (see paper)
47% identity, 98% coverage: 6:449/455 of query aligns to 2:439/441 of 6yu5A

query
sites
6yu5A

R

R

A

E

N

Q

F

W

G

A

S

S

K

R

L

L

G

G

V

F

I

I

L

L

A

A

S

A

A

M

G

G

S
|
S

A
|
A

V

V

G

G

L

L

G
|
G

N

N

I

I

W

W

R

R

F

F

P

S

F

Y

E

V

T

T

G

G

N

E

H

N

G

G

G

G

A

A

A

A

F

F

I

L

L

L

I

V

Y

Y

L

L

V

G

C

F

V

I

L

A

L

L

L

I

G

G

I

I

P

P

I

I

M

V

V

L

A

A

E

E

F

F

L

T

I

I

G

G

R

R

H

R

S

A

R

Q

A

S

N

D

T

A

A

V

G

G

A

S

Y

F

Q

E

R

K

L

L

A

A

P

P

G

G

T

K

R

P

W

W

R

K

W

V

V

A

G

G

R

L

M

M

G

G

V

V

L

A

A

A

G

G

F

F

L

L

I

I

L

L

G

S

Y

F

Y
|
Y

S

G

V

V

V

I

A

A

G

G

W

W

T

I

L

L

E

F

F

Y

I

L

G

F

E

N

A

Y

A

I

T

T

N

G

S

Q

F

L

A

W

G

S

K

A

S

P

A

A

A

E

D

G

F

F

I

G

A

G

S

F

F

F

N

E

S

G

F

F

T

I

G

A

N

N

P

P

W

T

R

L

P

P

I

L

V

F

W

W

L

Q

M

A

L

L

F

F

L

M

L

I

A

A

T

T

H

I

F

W

I

I

I

V

V

A

K

I

G

G

V

V

E

K

K

K

G

G

I

I

E

E

K

R

S

S

S

N

K

K

I

I

M

L

M

M

P

P

V

L

L

L

F

G

I

V

L

L

L

L

I

I

V

A

L

L

A

A

V

I

C

Y

S

S

V

L

S

T

L

L

P

G

G

G

A

A

G

K

A

E

G

G

I

L

E

A

F

F

L

L

L

F

K

S

P

P

D

D

F

W

S

S

K

A

V

L

-

K

D

D

G

P

N

G

V

V

L

Y

L

L

G

A

A

A

M

I

G

S

Q

Q

A

A

F

F

F
|
F

S
x
T

L

L

S
|
S

L

L

G

G

M

M

G

G

C

A

L

L

C

I

T

T

Y

Y

A

G

S

S

Y

Y

F

V

R

S

D

K

D

D

T

S

N

R

L

L

T

P

K

G

T

A

A

A

F

V

N

S

V

V

A

A

A

G

I

L

D

D

T

T

M

A

V

F

A

A

I

I

L

I

A

A

G

G

F

I

I

M

I

I

F

F

P

P

A

A

A

V

F

F

S

A

V

L

G

G

I

L

Q

S

P

P

D

S

A

G

G

G

P

P

S

G

L

L

L

V

F

F

I

V

T

V

L

L

P

P

N

D

V

I

F

F

Q

D

Q

S

A

I

F

R

G

-

N

-

L

-

P

-

W

-

L

L

A

G

V

P

I

I

L

V

S

G

L

I

M

A

F

F

Y

F

I

I

L

L

L

L

A

G

L

A

A

A

A

A

L

L

T

S

S
|
S

T

A

I

V

S

S

L

L

H

L

E

E

V

V

V

P

T

V

A

A

Y

Y

L

F

H

M

E

R

E

K

F

F

K

D

F

W

S

S

R

R

S

K

K

Q

A

A

A

A

R

-

L

-

V

I

T

T

A

L

G

G

C

V

I

I

-

I

-

T

F

L

L

L

G

G

V

I

F

P

C

S

S

S

L

L

S

S

L

F

G

G

V

V

G

L

K

G

D

E

Y

V

T

T

I

I

F

I

-

P

G

G

L

L

N

N

L

I

F

F

D

D

S

S

F

V

D

D

F

F

I

I

T

A

A

S

K

S

I

V

M

F

L

L

P

P

L

L

G

G

G

G

F

M

F

I

I

I

S

A

I

L

F

F

T

I

G

G

W

W

Y

G

L

W

D

K

K

T

K

S

I

D

V

A

W

L

E

A

E

E

V

S

S

D

N

L

N

T

G

D

S

S

L

V

K

W

V

-

P

-

V

-

Y

G

K

K

T

L

L

W

I

I

F

L

I

S

L

L

K

R

F

F

I

I

A

A

P

P

I

I

G

A

I

I

A

L

A

I

I

V

F

F

I

L

N

S

binding valine: S18 (= S22), A19 (= A23), G23 (= G27), Y101 (= Y105), F223 (= F226), T224 (≠ S227), S226 (= S229), S317 (= S325)

6yu3A Crystal structure of mhst in complex with l-phenylalanine (see paper)
47% identity, 98% coverage: 6:449/455 of query aligns to 2:439/441 of 6yu3A

query
sites
6yu3A

R

R

A

E

N

Q

F

W

G

A

S

S

K

R

L

L

G

G

V

F

I

I

L

L

A

A

S

A

A

M

G

G

S
|
S

A
|
A

V

V

G
|
G

L

L

G
|
G

N

N

I

I

W

W

R

R

F

F

P

S

F

Y

E

V

T

T

G

G

N

E

H

N

G

G

G

G

A

A

A

A

F

F

I

L

L

L

I

V

Y

Y

L

L

V

G

C

F

V

I

L

A

L

L

L

I

G

G

I

I

P

P

I

I

M

V

V

L

A

A

E

E

F

F

L

T

I

I

G

G

R

R

H

R

S

A

R

Q

A

S

N

D

T

A

A

V

G

G

A

S

Y

F

Q

E

R

K

L

L

A

A

P

P

G

G

T

K

R

P

W

W

R

K

W

V

V

A

G

G

R

L

M

M

G

G

V

V

L

A

A

A

G

G

F

F

L

L

I

I

L

L

G

S

Y

F

Y
|
Y

S

G

V

V

V

I

A

A

G

G

W

W

T

I

L

L

E

F

F

Y

I

L

G

F

E

N

A

Y

A

I

T

T

N

G

S

Q

F

L

A

W

G

S

K

A

S

P

A

A

A

E

D

G

F

F

I

G

A

G

S

F

F

F

N

E

S

G

F

F

T

I

G

A

N

N

P

P

W

T

R

L

P

P

I

L

V

F

W

W

L

Q

M

A

L

L

F

F

L

M

L

I

A

A

T

T

H

I

F

W

I

I

I

V

V

A

K

I

G

G

V

V

E

K

K

K

G

G

I

I

E

E

K

R

S

S

S

N

K

K

I

I

M

L

M

M

P

P

V

L

L

L

F

G

I

V

L

L

L

L

I

I

V

A

L

L

A

A

V

I

C

Y

S

S

V

L

S

T

L

L

P

G

G

G

A

A

G

K

A

E

G

G

I

L

E

A

F

F

L

L

L

F

K

S

P

P

D

D

F

W

S

S

K

A

V

L

-

K

D

D

G

P

N

G

V

V

L

Y

L

L

G

A

A

A

M

I

G

S

Q

Q

A

A

F

F

F
|
F

S
x
T

L

L

S
|
S

L

L

G

G

M
|
M

G

G

C

A

L

L

C

I

T

T

Y

Y

A

G

S

S

Y

Y

F

V

R

S

D

K

D

D

T

S

N

R

L

L

T

P

K

G

T

A

A

A

F

V

N

S

V

V

A

A

A

G

I

L

D

D

T

T

M

A

V

F

A

A

I

I

L

I

A

A

G

G

F

I

I

M

I

I

F

F

P

P

A

A

A

V

F

F

S

A

V

L

G

G

I

L

Q

S

P

P

D

S

A

G

G

G

P

P

S

G

L

L

L

V

F

F

I

V

T

V

L

L

P

P

N

D

V

I

F

F

Q

D

Q

S

A

I

F

R

G

-

N

-

L

-

P

-

W

-

L

L

A

G

V

P

I

I

L

V

S

G

L

I

M

A

F

F

Y

F

I

I

L

L

L

L

A

G

L

A

A

A

A

A

L

L

T

S

S

S

T

A

I

V

S

S

L
|
L

H

L

E

E

V

V

V

P

T

V

A

A

Y

Y

L

F

H

M

E

R

E

K

F

F

K

D

F

W

S

S

R

R

S

K

K

Q

A

A

A

A

R

-

L

-

V

I

T

T

A

L

G

G

C

V

I

I

-

I

-

T

F

L

L

L

G

G

V

I

F

P

C

S

S

S

L

L

S

S

L

F

G

G

V

V

G

L

K

G

D

E

Y

V

T

T

I

I

F

I

-

P

G

G

L

L

N

N

L

I

F

F

D

D

S

S

F

V

D

D

F

F

I

I

T

A

A

S

K

S

I

V

M

F

L

L

P

P

L

L

G

G

G

G

F

M

F

I

I

I

S

A

I

L

F

F

T

I

G

G

W

W

Y

G

L

W

D

K

K

T

K

S

I

D

V

A

W

L

E

A

E

E

V

S

S

D

N

L

N

T

G

D

S

S

L

V

K

W

V

-

P

-

V

-

Y

G

K

K

T

L

L

W

I

I

F

L

I

S

L

L

K

R

F

F

I

I

A

A

P

P

I

I

G

A

I

I

A

L

A

I

I

V

F

F

I

L

N

S

binding phenylalanine: S18 (= S22), A19 (= A23), G21 (= G25), G23 (= G27), Y101 (= Y105), F223 (= F226), T224 (≠ S227), S226 (= S229), M229 (= M232), L321 (= L329)

6yu2A Crystal structure of mhst in complex with l-isoleucine (see paper)
47% identity, 98% coverage: 6:449/455 of query aligns to 2:439/441 of 6yu2A

query
sites
6yu2A

R

R

A

E

N

Q

F

W

G

A

S

S

K

R

L

L

G

G

V

F

I

I

L

L

A

A

S

A

A

M

G

G

S
|
S

A
|
A

V

V

G
|
G

L

L

G
|
G

N

N

I

I

W

W

R

R

F

F

P

S

F

Y

E

V

T

T

G

G

N

E

H

N

G

G

G

G

A

A

A

A

F

F

I

L

L

L

I

V

Y

Y

L

L

V

G

C

F

V

I

L

A

L

L

L

I

G

G

I

I

P

P

I

I

M

V

V

L

A

A

E

E

F

F

L

T

I

I

G

G

R

R

H

R

S

A

R

Q

A

S

N

D

T

A

A

V

G

G

A

S

Y

F

Q

E

R

K

L

L

A

A

P

P

G

G

T

K

R

P

W

W

R

K

W

V

V

A

G

G

R

L

M

M

G

G

V

V

L

A

A

A

G

G

F

F

L

L

I

I

L

L

G

S

Y

F

Y
|
Y

S

G

V

V

V

I

A

A

G

G

W

W

T

I

L

L

E

F

F

Y

I

L

G

F

E

N

A

Y

A

I

T

T

N

G

S

Q

F

L

A

W

G

S

K

A

S

P

A

A

A

E

D

G

F

F

I

G

A

G

S

F

F

F

N

E

S

G

F

F

T

I

G

A

N

N

P

P

W

T

R

L

P

P

I

L

V

F

W

W

L

Q

M

A

L

L

F

F

L

M

L

I

A

A

T

T

H

I

F

W

I

I

I

V

V

A

K

I

G

G

V

V

E

K

K

K

G

G

I

I

E

E

K

R

S

S

S

N

K

K

I

I

M

L

M

M

P

P

V

L

L

L

F

G

I

V

L

L

L

L

I

I

V

A

L

L

A

A

V

I

C

Y

S

S

V

L

S

T

L

L

P

G

G

G

A

A

G

K

A

E

G

G

I

L

E

A

F

F

L

L

L

F

K

S

P

P

D

D

F

W

S

S

K

A

V

L

-

K

D

D

G

P

N

G

V

V

L

Y

L

L

G

A

A

A

M

I

G

S

Q

Q

A

A

F

F

F
|
F

S

T

L

L

S

S

L

L

G

G

M
|
M

G

G

C

A

L

L

C

I

T

T

Y

Y

A

G

S

S

Y

Y

F

V

R

S

D

K

D

D

T

S

N

R

L

L

T

P

K

G

T

A

A

A

F

V

N

S

V

V

A

A

A

G

I

L

D

D

T

T

M

A

V

F

A

A

I

I

L

I

A

A

G

G

F

I

I

M

I

I

F

F

P

P

A

A

A

V

F

F

S

A

V

L

G

G

I

L

Q

S

P

P

D

S

A

G

G

G

P

P

S

G

L

L

L

V

F

F

I

V

T

V

L

L

P

P

N

D

V

I

F

F

Q

D

Q

S

A

I

F

R

G

-

N

-

L

-

P

-

W

-

L

L

A

G

V

P

I

I

L

V

S

G

L

I

M

A

F

F

Y

F

I

I

L

L

L

L

A

G

L

A

A

A

A

A

L

L

T

S

S
|
S

T

A

I

V

S

S

L

L

H

L

E

E

V

V

V

P

T

V

A

A

Y

Y

L

F

H

M

E

R

E

K

F

F

K

D

F

W

S

S

R

R

S

K

K

Q

A

A

A

A

R

-

L

-

V

I

T

T

A

L

G

G

C

V

I

I

-

I

-

T

F

L

L

L

G

G

V

I

F

P

C

S

S

S

L

L

S

S

L

F

G

G

V

V

G

L

K

G

D

E

Y

V

T

T

I

I

F

I

-

P

G

G

L

L

N

N

L

I

F

F

D

D

S

S

F

V

D

D

F

F

I

I

T

A

A

S

K

S

I

V

M

F

L

L

P

P

L

L

G

G

G

G

F

M

F

I

I

I

S

A

I

L

F

F

T

I

G

G

W

W

Y

G

L

W

D

K

K

T

K

S

I

D

V

A

W

L

E

A

E

E

V

S

S

D

N

L

N

T

G

D

S

S

L

V

K

W

V

-

P

-

V

-

Y

G

K

K

T

L

L

W

I

I

F

L

I

S

L

L

K

R

F

F

I

I

A

A

P

P

I

I

G

A

I

I

A

L

A

I

I

V

F

F

I

L

N

S

binding isoleucine: S18 (= S22), A19 (= A23), G21 (= G25), G23 (= G27), Y101 (= Y105), F223 (= F226), M229 (= M232), S317 (= S325)

4us3A Crystal structure of the bacterial nss member mhst in an occluded inward-facing state (see paper)
47% identity, 98% coverage: 6:449/455 of query aligns to 2:439/441 of 4us3A

query
sites
4us3A

R

R

A

E

N

Q

F

W

G

A

S

S

K

R

L

L

G

G

V

F

I

I

L

L

A

A

S

A

A

M

G
|
G

S
|
S

A
|
A

V
|
V

G
|
G

L
|
L

G
|
G

N
|
N

I

I

W

W

R

R

F

F

P

S

F

Y

E

V

T

T

G

G

N

E

H

N

G

G

G

G

A

A

A

A

F

F

I

L

L

L

I

V

Y

Y

L

L

V

G

C

F

V

I

L

A

L

L

L

I

G

G

I

I

P

P

I

I

M

V

V

L

A

A

E

E

F

F

L

T

I

I

G

G

R

R

H

R

S

A

R

Q

A

S

N

D

T

A

A

V

G

G

A

S

Y

F

Q

E

R

K

L

L

A

A

P

P

G

G

T

K

R

P

W

W

R

K

W

V

V

A

G

G

R

L

M

M

G

G

V

V

L

A

A

A

G

G

F

F

L

L

I

I

L

L

G

S

Y

F

Y
|
Y

S

G

V

V

V

I

A

A

G

G

W

W

T

I

L

L

E

F

F

Y

I

L

G

F

E

N

A

Y

A

I

T

T

N

G

S

Q

F

L

A

W

G

S

K

A

S

P

A

A

A

E

D

G

F

F

I

G

A

G

S

F

F

F

N

E

S

G

F

F

T

I

G

A

N

N

P

P

W

T

R

L

P

P

I

L

V

F

W

W

L

Q

M

A

L

L

F

F

L

M

L

I

A

A

T

T

H

I

F

W

I

I

I

V

V

A

K

I

G

G

V

V

E

K

K

K

G

G

I

I

E

E

K

R

S

S

S

N

K

K

I

I

M

L

M

M

P

P

V

L

L

L

F

G

I

V

L

L

L

L

I

I

V

A

L

L

A

A

V

I

C

Y

S

S

V

L

S

T

L

L

P

G

G

G

A

A

G

K

A

E

G

G

I

L

E

A

F

F

L

L

L

F

K

S

P

P

D

D

F

W

S

S

K

A

V

L

-

K

D

D

G

P

N

G

V

V

L

Y

L

L

G

A

A

A

M

I

G

S

Q

Q

A

A

F

F

F
|
F

S
x
T

L

L

S
|
S

L

L

G

G

M
|
M

G

G

C

A

L

L

C

I

T

T

Y

Y

A

G

S

S

Y

Y

F

V

R

S

D

K

D

D

T

S

N

R

L

L

T

P

K

G

T

A

A

A

F

V

N

S

V

V

A

A

A

G

I

L

D
|
D

T

T

M

A

V

F

A

A

I

I

L

I

A

A

G

G

F

I

I

M

I

I

F

F

P

P

A

A

A

V

F

F

S

A

V

L

G

G

I

L

Q

S

P

P

D

S

A

G

G

G

P

P

S

G

L

L

L

V

F

F

I

V

T

V

L

L

P

P

N

D

V

I

F

F

Q

D

Q

S

A

I

F

R

G

-

N

-

L

-

P

-

W

-

L

L

A

G

V

P

I

I

L

V

S

G

L

I

M

A

F

F

Y

F

I

I

L

L

L

L

A

G

L

A

A
|
A

A

A

L

L

T
x
S

S
|
S

T

A

I

V

S
|
S

L
|
L

H

L

E

E

V

V

V

P

T

V

A

A

Y

Y

L

F

H

M

E

R

E

K

F

F

K

D

F

W

S

S

R

R

S

K

K

Q

A

A

A

A

R

-

L

-

V

I

T

T

A

L

G

G

C

V

I

I

-

I

-

T

F

L

L

L

G

G

V

I

F

P

C

S

S

S

L

L

S

S

L

F

G

G

V

V

G

L

K

G

D

E

Y

V

T

T

I

I

F

I

-

P

G

G

L

L

N

N

L

I

F

F

D

D

S

S

F

V

D

D

F

F

I

I

T

A

A

S

K

S

I

V

M

F

L

L

P

P

L

L

G

G

G

G

F

M

F

I

I

I

S

A

I

L

F

F

T

I

G

G

W

W

Y

G

L

W

D

K

K

T

K

S

I

D

V

A

W

L

E

A

E

E

V

S

S

D

N

L

N

T

G

D

S

S

L

V

K

W

V

-

P

-

V

-

Y

G

K

K

T

L

L

W

I

I

F

L

I

S

L

L

K

R

F

F

I

I

A

A

P

P

I

I

G

A

I

I

A

L

A

I

I

V

F

F

I

L

N

S

binding sodium ion: G17 (= G21), A19 (= A23), V20 (= V24), V20 (= V24), G21 (= G25), N24 (= N28), T224 (≠ S227), D256 (= D259), A313 (= A321), S316 (≠ T324), S317 (= S325)
binding tryptophan: S18 (= S22), A19 (= A23), L22 (= L26), G23 (= G27), Y101 (= Y105), F223 (= F226), T224 (≠ S227), S226 (= S229), M229 (= M232), S320 (= S328), L321 (= L329)

6yu7A Crystal structure of mhst in complex with l-tyrosine (see paper)
47% identity, 98% coverage: 6:449/455 of query aligns to 2:439/442 of 6yu7A

query
sites
6yu7A

R

R

A

E

N

Q

F

W

G

A

S

S

K

R

L

L

G

G

V

F

I

I

L

L

A

A

S

A

A

M

G

G

S
|
S

A
|
A

V

V

G
|
G

L

L

G
|
G

N

N

I

I

W

W

R

R

F

F

P

S

F

Y

E

V

T

T

G

G

N

E

H

N

G

G

G

G

A

A

A

A

F

F

I

L

L

L

I

V

Y

Y

L

L

V

G

C

F

V

I

L

A

L

L

L

I

G

G

I

I

P

P

I

I

M

V

V

L

A

A

E

E

F

F

L

T

I

I

G

G

R

R

H

R

S

A

R

Q

A

S

N

D

T

A

A

V

G

G

A

S

Y

F

Q

E

R

K

L

L

A

A

P

P

G

G

T

K

R

P

W

W

R

K

W

V

V

A

G

G

R

L

M

M

G

G

V

V

L

A

A

A

G

G

F

F

L

L

I

I

L

L

G

S

Y

F

Y
|
Y

S

G

V

V

V

I

A

A

G

G

W

W

T

I

L

L

E

F

F

Y

I

L

G

F

E

N

A

Y

A

I

T

T

N

G

S

Q

F

L

A

W

G

S

K

A

S

P

A

A

A

E

D

G

F

F

I

G

A

G

S

F

F

F

N

E

S

G

F

F

T

I

G

A

N

N

P

P

W

T

R

L

P

P

I

L

V

F

W

W

L

Q

M

A

L

L

F

F

L

M

L

I

A

A

T

T

H

I

F

W

I

I

I

V

V

A

K

I

G

G

V

V

E

K

K

K

G

G

I

I

E

E

K

R

S

S

S

N

K

K

I

I

M

L

M

M

P

P

V

L

L

L

F

G

I

V

L

L

L

L

I

I

V

A

L

L

A

A

V

I

C

Y

S

S

V

L

S

T

L

L

P

G

G

G

A

A

G

K

A

E

G

G

I

L

E

A

F

F

L

L

L

F

K

S

P

P

D

D

F

W

S

S

K

A

V

L

-

K

D

D

G

P

N

G

V

V

L

Y

L

L

G

A

A

A

M

I

G

S

Q

Q

A

A

F

F

F
|
F

S
x
T

L

L

S
|
S

L

L

G

G

M
|
M

G

G

C

A

L

L

C

I

T

T

Y

Y

A

G

S

S

Y

Y

F

V

R

S

D

K

D

D

T

S

N

R

L

L

T

P

K

G

T

A

A

A

F

V

N

S

V

V

A

A

A

G

I

L

D

D

T

T

M

A

V

F

A

A

I

I

L

I

A

A

G

G

F

I

I

M

I

I

F

F

P

P

A

A

A

V

F

F

S

A

V

L

G

G

I

L

Q

S

P

P

D

S

A

G

G

G

P

P

S

G

L

L

L

V

F

F

I

V

T

V

L

L

P

P

N

D

V

I

F

F

Q

D

Q

S

A

I

F

R

G

-

N

-

L

-

P

-

W

-

L

L

A

G

V

P

I

I

L

V

S

G

L

I

M

A

F

F

Y

F

I

I

L

L

L

L

A

G

L

A

A

A

A

A

L

L

T

S

S

S

T

A

I

V

S

S

L
|
L

H

L

E

E

V

V

V

P

T

V

A

A

Y

Y

L

F

H

M

E

R

E

K

F

F

K

D

F

W

S

S

R

R

S

K

K

Q

A

A

A

A

R

-

L

-

V

I

T

T

A

L

G

G

C

V

I

I

-

I

-

T

F

L

L

L

G

G

V

I

F

P

C

S

S

S

L

L

S

S

L

F

G

G

V

V

G

L

K

G

D

E

Y

V

T

T

I

I

F

I

-

P

G

G

L

L

N

N

L

I

F

F

D

D

S

S

F

V

D

D

F

F

I

I

T

A

A

S

K

S

I

V

M

F

L

L

P

P

L

L

G

G

G

G

F

M

F

I

I

I

S

A

I

L

F

F

T

I

G

G

W

W

Y

G

L

W

D

K

K

T

K

S

I

D

V

A

W

L

E

A

E

E

V

S

S

D

N

L

N

T

G

D

S

S

L

V

K

W

V

-

P

-

V

-

Y

G

K

K

T

L

L

W

I

I

F

L

I

S

L

L

K

R

F

F

I

I

A

A

P

P

I

I

G

A

I

I

A

L

A

I

I

V

F

F

I

L

N

S

binding tyrosine: S18 (= S22), A19 (= A23), G21 (= G25), G23 (= G27), Y101 (= Y105), F223 (= F226), T224 (≠ S227), S226 (= S229), M229 (= M232), L321 (= L329)

6yu4A Crystal structure of mhst in complex with l-4f-phenylalanine (see paper)
47% identity, 98% coverage: 6:449/455 of query aligns to 2:439/442 of 6yu4A

query
sites
6yu4A

R

R

A

E

N

Q

F

W

G

A

S

S

K

R

L

L

G

G

V

F

I

I

L

L

A

A

S

A

A

M

G

G

S
|
S

A
|
A

V

V

G
|
G

L
|
L

G
|
G

N

N

I

I

W

W

R

R

F

F

P

S

F

Y

E

V

T

T

G

G

N

E

H

N

G

G

G

G

A

A

A

A

F

F

I

L

L

L

I

V

Y

Y

L

L

V

G

C

F

V

I

L

A

L

L

L

I

G

G

I

I

P

P

I

I

M

V

V

L

A

A

E

E

F

F

L

T

I

I

G

G

R

R

H

R

S

A

R

Q

A

S

N

D

T

A

A

V

G

G

A

S

Y

F

Q

E

R

K

L

L

A

A

P

P

G

G

T

K

R

P

W

W

R

K

W

V

V

A

G

G

R

L

M

M

G

G

V

V

L

A

A

A

G

G

F

F

L

L

I

I

L

L

G

S

Y

F

Y
|
Y

S

G

V

V

V

I

A

A

G

G

W

W

T

I

L

L

E

F

F

Y

I

L

G

F

E

N

A

Y

A

I

T

T

N

G

S

Q

F

L

A

W

G

S

K

A

S

P

A

A

A

E

D

G

F

F

I

G

A

G

S

F

F

F

N

E

S

G

F

F

T

I

G

A

N

N

P

P

W

T

R

L

P

P

I

L

V

F

W

W

L

Q

M

A

L

L

F

F

L

M

L

I

A

A

T

T

H

I

F

W

I

I

I

V

V

A

K

I

G

G

V

V

E

K

K

K

G

G

I

I

E

E

K

R

S

S

S

N

K

K

I

I

M

L

M

M

P

P

V

L

L

L

F

G

I

V

L

L

L

L

I

I

V

A

L

L

A

A

V

I

C

Y

S

S

V

L

S

T

L

L

P

G

G

G

A

A

G

K

A

E

G

G

I

L

E

A

F

F

L

L

L

F

K

S

P

P

D

D

F

W

S

S

K

A

V

L

-

K

D

D

G

P

N

G

V

V

L

Y

L

L

G

A

A

A

M

I

G

S

Q

Q

A

A

F

F

F
|
F

S
x
T

L

L

S
|
S

L

L

G

G

M
|
M

G

G

C

A

L

L

C

I

T

T

Y

Y

A

G

S

S

Y

Y

F

V

R

S

D

K

D

D

T

S

N

R

L

L

T

P

K

G

T

A

A

A

F

V

N

S

V

V

A

A

A

G

I

L

D

D

T

T

M

A

V

F

A

A

I

I

L

I

A

A

G

G

F

I

I

M

I

I

F

F

P

P

A

A

A

V

F

F

S

A

V

L

G

G

I

L

Q

S

P

P

D

S

A

G

G

G

P

P

S

G

L

L

L

V

F

F

I

V

T

V

L

L

P

P

N

D

V

I

F

F

Q

D

Q

S

A

I

F

R

G

-

N

-

L

-

P

-

W

-

L

L

A

G

V

P

I

I

L

V

S

G

L

I

M

A

F

F

Y

F

I

I

L

L

L

L

A

G

L

A

A

A

A

A

L

L

T

S

S

S

T

A

I

V

S

S

L
|
L

H

L

E

E

V

V

V

P

T

V

A

A

Y

Y

L

F

H

M

E

R

E

K

F

F

K

D

F

W

S

S

R

R

S

K

K

Q

A

A

A

A

R

-

L

-

V

I

T

T

A

L

G

G

C

V

I

I

-

I

-

T

F

L

L

L

G

G

V

I

F

P

C

S

S

S

L

L

S

S

L

F

G

G

V

V

G

L

K

G

D

E

Y

V

T

T

I

I

F

I

-

P

G

G

L

L

N

N

L

I

F

F

D

D

S

S

F

V

D

D

F

F

I

I

T

A

A

S

K

S

I

V

M

F

L

L

P

P

L

L

G

G

G

G

F

M

F

I

I

I

S

A

I

L

F

F

T

I

G

G

W

W

Y

G

L

W

D

K

K

T

K

S

I

D

V

A

W

L

E

A

E

E

V

S

S

D

N

L

N

T

G

D

S

S

L

V

K

W

V

-

P

-

V

-

Y

G

K

K

T

L

L

W

I

I

F

L

I

S

L

L

K

R

F

F

I

I

A

A

P

P

I

I

G

A

I

I

A

L

A

I

I

V

F

F

I

L

N

S

binding 4-fluoro-l-phenylalanine: S18 (= S22), A19 (= A23), G21 (= G25), L22 (= L26), G23 (= G27), Y101 (= Y105), F223 (= F226), T224 (≠ S227), S226 (= S229), M229 (= M232), L321 (= L329)

6yu6B Crystal structure of mhst in complex with l-leucine (see paper)
47% identity, 98% coverage: 6:449/455 of query aligns to 2:439/446 of 6yu6B

query
sites
6yu6B

R

R

A

E

N

Q

F

W

G

A

S

S

K

R

L

L

G

G

V

F

I

I

L

L

A

A

S

A

A

M

G

G

S
|
S

A
|
A

V

V

G
|
G

L

L

G
|
G

N

N

I

I

W

W

R

R

F

F

P

S

F

Y

E

V

T

T

G

G

N

E

H

N

G

G

G

G

A

A

A

A

F

F

I

L

L

L

I

V

Y

Y

L

L

V

G

C

F

V

I

L

A

L

L

L

I

G

G

I

I

P

P

I

I

M

V

V

L

A

A

E

E

F

F

L

T

I

I

G

G

R

R

H

R

S

A

R

Q

A

S

N

D

T

A

A

V

G

G

A

S

Y

F

Q

E

R

K

L

L

A

A

P

P

G

G

T

K

R

P

W

W

R

K

W

V

V

A

G

G

R

L

M

M

G

G

V

V

L

A

A

A

G

G

F

F

L

L

I

I

L

L

G

S

Y

F

Y

Y

S

G

V

V

V

I

A

A

G

G

W

W

T

I

L

L

E

F

F

Y

I

L

G

F

E

N

A

Y

A

I

T

T

N

G

S

Q

F

L

A

W

G

S

K

A

S

P

A

A

A

E

D

G

F

F

I

G

A

G

S

F

F

F

N

E

S

G

F

F

T

I

G

A

N

N

P

P

W

T

R

L

P

P

I

L

V

F

W

W

L

Q

M

A

L

L

F

F

L

M

L

I

A

A

T

T

H

I

F

W

I

I

I

V

V

A

K

I

G

G

V

V

E

K

K

K

G

G

I

I

E

E

K

R

S

S

S

N

K

K

I

I

M

L

M

M

P

P

V

L

L

L

F

G

I

V

L

L

L

L

I

I

V

A

L

L

A

A

V

I

C

Y

S

S

V

L

S

T

L

L

P

G

G

G

A

A

G

K

A

E

G

G

I

L

E

A

F

F

L

L

L

F

K

S

P

P

D

D

F

W

S

S

K

A

V

L

-

K

D

D

G

P

N

G

V

V

L

Y

L

L

G

A

A

A

M

I

G

S

Q

Q

A

A

F

F

F
|
F

S
x
T

L

L

S
|
S

L

L

G

G

M
|
M

G

G

C

A

L

L

C

I

T

T

Y

Y

A

G

S

S

Y

Y

F

V

R

S

D

K

D

D

T

S

N

R

L

L

T

P

K

G

T

A

A

A

F

V

N

S

V

V

A

A

A

G

I

L

D

D

T

T

M

A

V

F

A

A

I

I

L

I

A

A

G

G

F

I

I

M

I

I

F

F

P

P

A

A

A

V

F

F

S

A

V

L

G

G

I

L

Q

S

P

P

D

S

A

G

G

G

P

P

S

G

L

L

L

V

F

F

I

V

T

V

L

L

P

P

N

D

V

I

F

F

Q

D

Q

S

A

I

F

R

G

-

N

-

L

-

P

-

W

-

L

L

A

G

V

P

I

I

L

V

S

G

L

I

M

A

F

F

Y

F

I

I

L

L

L

L

A

G

L

A

A

A

A

A

L

L

T

S

S

S

T

A

I

V

S

S

L
|
L

H

L

E

E

V

V

V

P

T

V

A

A

Y

Y

L

F

H

M

E

R

E

K

F

F

K

D

F

W

S

S

R

R

S

K

K

Q

A

A

A

A

R

-

L

-

V

I

T

T

A

L

G

G

C

V

I

I

-

I

-

T

F

L

L

L

G

G

V

I

F

P

C

S

S

S

L

L

S

S

L

F

G

G

V

V

G

L

K

G

D

E

Y

V

T

T

I

I

F

I

-

P

G

G

L

L

N

N

L

I

F

F

D

D

S

S

F

V

D

D

F

F

I

I

T

A

A

S

K

S

I

V

M

F

L

L

P

P

L

L

G

G

G

G

F

M

F

I

I

I

S

A

I

L

F

F

T

I

G

G

W

W

Y

G

L

W

D

K

K

T

K

S

I

D

V

A

W

L

E

A

E

E

V

S

S

D

N

L

N

T

G

D

S

S

L

V

K

W

V

-

P

-

V

-

Y

G

K

K

T

L

L

W

I

I

F

L

I

S

L

L

K

R

F

F

I

I

A

A

P

P

I

I

G

A

I

I

A

L

A

I

I

V

F

F

I

L

N

S

binding leucine: S18 (= S22), A19 (= A23), G21 (= G25), G23 (= G27), F223 (= F226), T224 (≠ S227), S226 (= S229), M229 (= M232), L321 (= L329)

4us4A Crystal structure of the bacterial nss member mhst in an occluded inward-facing state (lipidic cubic phase form) (see paper)
47% identity, 96% coverage: 14:449/455 of query aligns to 1:430/433 of 4us4A

query
sites
4us4A

G

G

V

F

I

I

L

L

A

A

S

A

A

M

G
|
G

S
|
S

A
|
A

V
|
V

G
|
G

L

L

G
|
G

N
|
N

I

I

W

W

R

R

F

F

P

S

F

Y

E

V

T

T

G

G

N

E

H

N

G

G

G

G

A

A

A

A

F

F

I

L

L

L

I

V

Y

Y

L

L

V

G

C

F

V

I

L

A

L

L

L

I

G

G

I

I

P

P

I

I

M

V

V

L

A

A

E

E

F

F

L

T

I

I

G

G

R

R

H

R

S

A

R

Q

A

S

N

D

T

A

A

V

G

G

A

S

Y

F

Q

E

R

K

L

L

A

A

P

P

G

G

T

K

R

P

W

W

R

K

W

V

V

A

G

G

R

L

M

M

G

G

V

V

L

A

A

A

G

G

F

F

L

L

I

I

L

L

G

S

Y

F

Y
|
Y

S

G

V

V

V

I

A

A

G

G

W

W

T

I

L

L

E

F

F

Y

I

L

G

F

E

N

A

Y

A

I

T

T

N

G

S

Q

F

L

A

W

G

S

K

A

S

P

A

A

A

E

D

G

F

F

I

G

A

G

S

F

F

F

N

E

S

G

F

F

T

I

G

A

N

N

P

P

W

T

R

L

P

P

I

L

V

F

W

W

L

Q

M

A

L

L

F

F

L

M

L

I

A

A

T

T

H

I

F

W

I

I

I

V

V

A

K

I

G

G

V

V

E

K

K

K

G

G

I

I

E

E

K

R

S

S

S

N

K

K

I

I

M

L

M

M

P

P

V

L

L

L

F

G

I

V

L

L

L

L

I

I

V
x
A

L

L

A

A

V

I

C

Y

S

S

V

L

S
x
T

L

L

P

G

G

G

A

A

G

K

A

E

G

G

I

L

E

A

F

F

L

L

L

F

K

S

P

P

D

D

F

W

S

S

K

A

V

L

-

K

D

D

G

P

N

G

V

V

L

Y

L

L

G

A

A

A

M

I

G

S

Q

Q

A

A

F

F

F
|
F

S
x
T

L

L

S
|
S

L

L

G

G

M
|
M

G

G

C

A

L

L

C

I

T

T

Y

Y

A

G

S

S

Y

Y

F

V

R

S

D

K

D

D

T

S

N

R

L

L

T

P

K

G

T

A

A

A

F

V

N

S

V

V

A

A

A

G

I

L

D
|
D

T

T

M

A

V

F

A

A

I

I

L

I

A

A

G

G

F

I

I

M

I

I

F

F

P

P

A

A

A

V

F

F

S

A

V

L

G

G

I

L

Q

S

P

P

D

S

A

G

G

G

P

P

S

G

L

L

L

V

F

F

I

V

T

V

L

L

P

P

N

D

V

I

F

F

Q

D

Q

S

A
x
I

F
x
R

G

-

N

-

L

-

P

-

W

-

L
|
L

A

G

V

P

I

I

L

V

S

G

L

I

M

A

F

F

Y

F

I

I

L

L

L

L

A

G

L

A

A
|
A

A

A

L

L

T
x
S

S
|
S

T

A

I

V

S

S

L
|
L

H

L

E

E

V

V

V

P

T

V

A

A

Y

Y

L

F

H

M

E

R

E

K

F

F

K

D

F

W

S

S

R

R

S

K

K

Q

A

A

A

A

R

-

L

-

V

I

T

T

A

L

G

G

C

V

I

I

-

I

-

T

F

L

L

L

G

G

V

I

F

P

C

S

S

S

L

L

S

S

L

F

G

G

V

V

G

L

K

G

D

E

Y

V

T

T

I

I

F

I

-

P

G

G

L

L

N

N

L

I

F

F

D

D

S

S

F

V

D

D

F

F

I

I

T

A

A

S

K

S

I

V

M

F

L

L

P

P

L

L

G

G

G

G

F

M

F

I

I

I

S

A

I

L

F

F

T

I

G

G

W

W

Y

G

L

W

D

K

K

T

K

S

I

D

V

A

W

L

E

A

E

E

V

S

S

D

N

L

N

T

G

D

S

S

L

V

K

W

V

-

P

-

V

-

Y

G

K

K

T

L

L

W

I

I

F

L

I

S

L

L

K

R

F

F

I

I

A

A

P

P

I

I

G

A

I

I

A

L

A

I

I

V

F

F

I

L

N

S

binding (2s)-2,3-dihydroxypropyl(7z)-pentadec-7-enoate: A173 (≠ V186), T180 (≠ S193), I287 (≠ A299), R288 (≠ F300), L289 (= L306)
binding sodium ion: G8 (= G21), S9 (= S22), A10 (= A23), V11 (= V24), V11 (= V24), N15 (= N28), T215 (≠ S227), D247 (= D259), A304 (= A321), S307 (≠ T324), S308 (= S325)
binding tryptophan: S9 (= S22), A10 (= A23), G12 (= G25), G14 (= G27), Y92 (= Y105), F214 (= F226), T215 (≠ S227), S217 (= S229), M220 (= M232), L312 (= L329)

3gwwA Leucine transporter leut in complex with s-fluoxetine (see paper)
32% identity, 97% coverage: 6:448/455 of query aligns to 1:456/501 of 3gwwA

query
sites
3gwwA

R

R

A

E

N

H

F

W

G

A

S

T

K

R

L

L

G

G

V

L

I

I

L

L

A

A

S

M

A

A

G

G

S

N

A
|
A

V

V

G
|
G

L
|
L

G
|
G

N

N

I

F

W

L

R
|
R

F

F

P

P

F

V

E

Q

T

A

G

A

N

E

H

N

G

G

G

G

A

G

A

A

F

F

I

M

L

I

I

P

Y

Y

L

I

V

I

C

A

V

F

L

L

L

L

L

V

G

G

I

I

P

P

I

L

M

M

V

W

A

I

E

E

F

W

L

A

I

M

G

G

R

R

H

Y

S

G

R

G

A

A

N

Q

-

G

-

H

-

G

-

T

T

T

A

P

G

A

A

I

Y

F

Q

Y

R

L

L

L

A

W

P

R

G

N

T

R

R

F

W

A

R

K

W

I

V

L

G

G

R

V

M

F

G

G

V

L

L

W

A

I

G

P

F

L

L

V

I

V

L

A

G

I

Y

Y

Y
|
Y

S

V

V

Y

V

I

A

E

G

S

W

W

T

T

L

L

E

G

F

F

I

A

G

I

E

K

A

F

A

L

T

V

N

G

S

L

F

V

A

P

G

E

K

P

S

P

A

P

A

D

D

S

F

I

I

L

A

R

S

P

F

F

N

K

-

E

-

F

-

L

-

Y

S

S

F

Y

T

I

G

G

N

V

P

P

-

K

-

G

-

D

-

E

-

P

-

I

W

L

R

K

P

P

I

S

V

L

W

F

L

A

M

Y

L

I

F

V

L

F

L

L

A

I

T

T

H

M

F

F

-

I

-

N

-

V

-

S

I

I

I

L

V

I

K

R

G

G

V

I

E

S

K

K

G

G

I

I

E

E

K

R

S

F

S

A

K

K

I

I

M

A

M

M

P

P

V

T

L

L

F

F

I

I

L

L

-

A

-

V

L

F

I

L

V

V

L

I

A

R

V

V

C

F

S

L

V

L

S

E

L

T

P

P

G

N

-

G

-

T

A

A

G

A

A

D

G

G

I

L

E

N

F

F

L

L

L

W

K

T

P

P

D

D

F

F

S

E

K

K

V

L

-

K

D

D

G

P

N

G

V

V

L

W

L

I

G

A

A

A

M

V

G

G

Q

Q

A

I

F

F

F
|
F

S
x
T

L

L

S
|
S

L

L

G

G

M
x
F

G

G

C

A

L

I

C

I

T

T

Y

Y

A

A

S

S

Y

Y

F

V

R

R

D

K

D

D

T

Q

N

D

L

I

T

V

K

L

T

S

A

G

F

L

N

T

V

A

A

A

A

T

I

L

D

N

T

E

M

K

V

A

A

E

-

V

I

I

L

L

A

G

G

G

F

S

I

I

I

S

F

I

P

P

A

A

A

A

F

V

-

A

-

F

-

F

-

G

-

V

-

A

-

N

S

A

V

V

G

A

I

I

Q

A

P

K

D

A

A

G

G
x
A

P
x
F

S

N

L

L

L

G

F

F

I

I

T

T

L

L

P

P

N

A

V

I

F

F

Q

S

Q

Q

A

T

F

A

G

G

N

G

L

-

P

-

W

-

L

-

A

-

V

T

I

F

L

L

S

G

L

F

M

L

F

W

Y

F

I

F

L

L

L

L

A

F

L

F

A

A

A

G

L

L

T

T

S

S

T

S

I

I

S

A

L
x
I

H

M

E

Q

V

P

V

M

T

I

A

A

Y

F

L

L

H

E

E

D

E

E

F

L

K

K

F

L

S

S

R

R

S

-

K

K

A

H

A

A

R

V

L

L

V

W

T

T

A

A

G

A

C

I

I

V

F

F

L

F

G

S

V

A

F

H

C

L

S

V

L

M

S

F

L

L

G

N

V

-

G

-

K

-

D

-

Y

-

T

-

I

-

F

-

G

-

L

-

N

K

L

S

F

L

D
|
D

S

E

F

M

D
|
D

F

F

I

W

T

A

A

G

K

T

I

I

M

G

L

V

P

V

L

F

G

F

G

G

F

L

F

T

I

E

S

L

I

I

F

I

T

F

G

F

W

W

Y

I

L

F

D

G

K

A

K

D

I

K

V

A

W

W

E

E

E

E

V

I

S

N

N

R

N

G

G

G

S

I

L

I

K

K

V

V

P

P

V

-

Y

-

K

R

T

I

L

Y

I

Y

F

Y

I

V

L

M

K

R

F

Y

I

I

A

T

P

P

I

A

G

F

I

L

A

A

A

V

I

L

F

L

I

V

binding leucine: A18 (= A23), G20 (= G25), G22 (= G27), Y104 (= Y105), F244 (= F226), T245 (≠ S227), S247 (= S229), F250 (≠ M232), I350 (≠ L329)
binding (3S)-N-methyl-3-phenyl-3-[4-(trifluoromethyl)phenoxy]propan-1-amine: L21 (= L26), G22 (= G27), R26 (= R31), Y104 (= Y105), A310 (≠ G284), F311 (≠ P285), D392 (= D382), D395 (= D385)

3uslA Crystal structure of leut bound to l-selenomethionine in space group c2 from lipid bicelles (see paper)
32% identity, 97% coverage: 6:448/455 of query aligns to 1:458/502 of 3uslA

query
sites
3uslA

R

R

A

E

N

H

F

W

G

A

S

T

K

R

L

L

G

G

V

L

I

I

L

L

A

A

S

M

A

A

G
|
G

S
x
N

A
|
A

V
|
V

G
|
G

L

L

G
|
G

N
|
N

I

F

W

L

R

R

F

F

P

P

F

V

E

Q

T

A

G

A

N

E

H

N

G

G

G

G

A

G

A

A

F

F

I

M

L

I

I

P

Y

Y

L

I

V

I

C

A

V

F

L

L

L

L

L

V

G

G

I

I

P

P

I

L

M

M

V

W

A

I

E

E

F

W

L

A

I

M

G

G

R

R

H

Y

S

G

R

G

A

A

N

Q

-

G

-

H

-

G

-

T

T

T

A

P

G

A

A

I

Y

F

Q

Y

R

L

L

L

A

W

P

R

G
x
N

T
x
R

R
x
F

W

A

R

K

W

I

V

L

G

G

R

V

M

F

G

G

V

L

L

W

A

I

G

P

F

L

L

V

I

V

L

A

G

I

Y

Y

Y
|
Y

S

V

V

Y

V

I

A

E

G

S

W

W

T

T

L

L

E

G

F

F

-

A

-

I

-

K

-

F

-

L

-

V

-

G

-

L

I

V

G

P

E

E

A

P

A

P

T

T

N

D

S

P

F

-

A

-

G

D

K

S

S

I

A

L

A

R

D

P

F

F

I

K

A

E

S

F

F

L

N

Y

S

S

F

Y

T

I

G

G

N

V

P

P

-

K

-

G

-

D

-

E

-

P

-

I

W

L

R

K

P

P

I

S

V

L

W

F

L

A

M

Y

L

I

F

V

L

F

L

L

A

I

T

T

H

M

F

F

-

I

-

N

-

V

-

S

I

I

I

L

V

I

K

R

G

G

V

I

E

S

K

K

G

G

I

I

E

E

K

R

S

F

S

A

K

K

I

I

M

A

M

M

P

P

V

T

L

L

F

F

I

I

L

L

-

A

-

V

L

F

I

L

V

V

L

I

A

R

V

V

C

F

S

L

V

L

S

E

L

T

P

P

G

N

-

G

-

T

A

A

G

A

A

D

G

G

I

L

E

N

F

F

L

L

L

W

K

T

P

P

D

D

F

F

S

E

K

K

V

L

-

K

D

D

G

P

N

G

V

V

L

W

L

I

G

A

A

A

M

V

G

G

Q

Q

A

I

F

F

F
|
F

S
x
T

L

L

S
|
S

L

L

G

G

M

F

G

G

C

A

L

I

C

I

T

T

Y

Y

A

A

S

S

Y

Y

F

V

R

R

D

K

D

D

T

Q

N

D

L

I

T

V

K

L

T

S

A

G

F

L

N

T

V

A

A

A

A

T

I

L

D
x
N

T

E

M

K

V

A

A

E

-

V

I

I

L

L

A

G

G

G

F

S

I

I

I

S

F

I

P

P

A

A

A

A

F

V

-

A

-

F

-

F

-

G

-

V

-

A

-

N

S

A

V

V

G

A

I

I

Q

A

P

K

D

A

A

G

G

A

P

F

S

N

L

L

L

G

F

F

I

I

T

T

L

L

P

P

N

A

V

I

F

F

Q

S

Q

Q

A

T

F

A

G

G

N

G

L

-

P

-

W

-

L

-

A

-

V

T

I

F

L

L

S

G

L

F

M

L

F

W

Y

F

I

F

L

L

L

L

A

F

L

F

A
|
A

A

G

L

L

T
|
T

S
|
S

T

S

I

I

S

A

L
x
I

H

M

E

Q

V

P

V

M

T

I

A

A

Y

F

L

L

H

E

E

D

E

E

F

L

K

K

F

L

S

S

R

R

S

-

K

K

A

H

A

A

R

V

L

L

V

W

T

T

A

A

G

A

C

I

I

V

F

F

L

F

G

S

V

A

F

H

C

L

S

V

L

M

S

F

L

L

G

N

V

-

G

-

K

-

D

-

Y

-

T

-

I

-

F

-

G

-

L

-

N

K

L

S

F

L

D

D

S

E

F

M

D

D

F

F

I

W

T

A

A

G

K

T

I

I

M

G

L

V

P

V

L

F

G

F

G

G

F

L

F

T

I

E

S

L

I

I

F

I

T

F

G

F

W

W

Y

I

L

F

D

G

K

A

K

D

I

K

V

A

W

W

E

E

E

E

V

I

S

N

N

R

N

G

G

G

S

I

L

I

K

K

V

V

P

P

V

-

Y

-

K

R

T

I

L

Y

I

Y

F

Y

I

V

L

M

K

R

F

Y

I

I

A

T

P

P

I

A

G

F

I

L

A

A

A

V

I

L

F

L

I

V

binding selenomethionine: A18 (= A23), G20 (= G25), G22 (= G27), Y104 (= Y105), F246 (= F226), T247 (≠ S227), S249 (= S229), S348 (= S325), I352 (≠ L329)
binding sodium ion: G16 (= G21), N17 (≠ S22), A18 (= A23), V19 (= V24), V19 (= V24), G20 (= G25), N23 (= N28), T247 (≠ S227), N279 (≠ D259), A344 (= A321), T347 (= T324), S348 (= S325)
binding phosphocholine: N83 (≠ G84), R84 (≠ T85), F85 (≠ R86)

3usgA Crystal structure of leut bound to l-leucine in space group c2 from lipid bicelles (see paper)
32% identity, 97% coverage: 6:448/455 of query aligns to 1:458/502 of 3usgA

query
sites
3usgA

R

R

A

E

N

H

F

W

G

A

S

T

K

R

L

L

G

G

V

L

I

I

L

L

A

A

S

M

A

A

G
|
G

S
x
N

A
|
A

V
|
V